CN112111402A - Culture system for cell biological electromagnetic effect evaluation - Google Patents
Culture system for cell biological electromagnetic effect evaluation Download PDFInfo
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- CN112111402A CN112111402A CN202011042936.2A CN202011042936A CN112111402A CN 112111402 A CN112111402 A CN 112111402A CN 202011042936 A CN202011042936 A CN 202011042936A CN 112111402 A CN112111402 A CN 112111402A
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- 230000005288 electromagnetic effect Effects 0.000 title claims abstract description 12
- 238000011156 evaluation Methods 0.000 title claims abstract description 8
- 238000004113 cell culture Methods 0.000 claims abstract description 63
- 238000001514 detection method Methods 0.000 claims abstract description 13
- 238000000034 method Methods 0.000 claims description 2
- 230000005672 electromagnetic field Effects 0.000 abstract description 14
- 230000004071 biological effect Effects 0.000 abstract description 10
- 230000000191 radiation effect Effects 0.000 abstract description 5
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 8
- 239000011148 porous material Substances 0.000 description 6
- 229910002092 carbon dioxide Inorganic materials 0.000 description 4
- 239000001569 carbon dioxide Substances 0.000 description 4
- 238000005457 optimization Methods 0.000 description 3
- 238000010586 diagram Methods 0.000 description 2
- 230000005611 electricity Effects 0.000 description 2
- 238000007789 sealing Methods 0.000 description 2
- 238000004088 simulation Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 230000003833 cell viability Effects 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000005670 electromagnetic radiation Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
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Abstract
The invention belongs to the field of research on biological effect of a pulse electromagnetic field. The culture system for cell biological electromagnetic effect evaluation is used for providing a constant-temperature and constant-humidity environment for cells, reducing the influence of an external environment on the cell biological effect and providing a basis for accurately evaluating the radiation effect of an electromagnetic field on the cell molecular level. The system comprises: a signal generator, a power amplifier and a cell culture box; the cell culture box comprises a box body, a bracket, a cell culture plate and a magnetic field detection sensor, wherein the bracket, the cell culture plate and the magnetic field detection sensor are arranged in the box body; the cell culture plate is arranged on the bracket, and plate holes which are uniformly distributed and used for containing cell culture solution are arranged on the cell culture plate; a layer of array coil is respectively arranged above and below the cell culture plate; the signal generator is electrically connected with the power amplifier, and the power amplifier is electrically connected with the array coil; the magnetic field detection sensor is electrically connected with the signal amplifier.
Description
Technical Field
The invention relates to a culture system for evaluating a biological electromagnetic effect of a cell, belonging to the field of research on biological effect of a pulse electromagnetic field.
Background
The biological effect of the alternating electromagnetic field on the cell level is the basis of researching the biological electromagnetic effect, and has certain guiding significance in the application aspects of the electromagnetic field to the treatment, prevention and the like of diseases.
The current routine method for assessing studies of the biological effects of an alternating electromagnetic field on cells is to expose the cells to an alternating electromagnetic field in an open environment. In an open environment, the temperature, the air humidity and the pH value in a culture solution can cause biological changes of cells to a certain extent, so that the biological effect of the alternating electromagnetic field on the cells is difficult to distinguish and research; in addition, as exposure time in an open environment increases, cell viability decreases.
Disclosure of Invention
The technical problem to be solved by the invention is as follows: the culture system for evaluating the biological electromagnetic effect of the cells provides a constant-temperature and constant-humidity environment for the cells, reduces the influence of the external environment on the biological effect of the cells, and provides a basis for accurately evaluating the radiation effect of an electromagnetic field on the molecular level of the cells.
The technical scheme adopted by the invention for solving the technical problems is as follows:
a culture system for the assessment of the biological electromagnetic effects of cells, comprising: a signal generator, a power amplifier and a cell culture box; the cell culture box comprises a box body, a bracket, a cell culture plate and a magnetic field detection sensor, wherein the bracket, the cell culture plate and the magnetic field detection sensor are arranged in the box body; the cell culture plate is arranged on the bracket, and plate holes which are uniformly distributed and used for containing cell culture solution are arranged on the cell culture plate; a layer of array coil is respectively arranged above and below the cell culture plate; the signal generator is electrically connected with the power amplifier and is used for generating a plurality of driving currents with different waveforms and adjustable intensity and frequency; the power amplifier is electrically connected with the array coil and is used for amplifying the driving current so as to drive the array coil to generate an alternating magnetic field acting on the cell culture plate; the magnetic field detection sensor is electrically connected with the signal generator and used for detecting the actual magnetic field intensity in the cell culture box and feeding back the actual magnetic field intensity to the signal generator to form closed-loop control and serve as the adjustment of the signal generator to the generated magnetic field intensity.
As a further optimization, the array coil comprises a plurality of circular coils connected in series, the number of the circular coils is equal to the number of the plate holes, and the inner diameters of the circular coils are equal to the inner diameters of the plate holes.
As a further optimization, circular coils in the array coils above and below the cell culture plate correspond to plate holes one by one, and the center of each circular coil is aligned with the center of the corresponding plate hole.
As a further optimization, the distances between the upper and lower array coils of the cell culture plate and the cell culture plate are equal, and the interval between the upper array coil and the lower array coil is equal to the radius of the circular coil.
The invention has the beneficial effects that:
based on the culture system designed above, the cell culture plate and the array coil are integrated in the box body and connected with an external power amplifier through a sealing port by a cable, so that cells are prevented from being exposed in an open alternating electromagnetic environment, and the influence of the external environment on the biological effect of the cells is reduced; and the upper and lower two-layer array coil's through the cell culture board setting constitutes a helmholtz coil array for the cell culture board that is located in the middle can obtain the better magnetic field environment of magnetic field homogeneity, guarantees the stability and the standardization of cell culture with this, provides the basis for accurate aassessment electromagnetic field to the radiation effect of cell molecule level.
Drawings
FIG. 1 is a schematic block diagram of a culture system according to the present invention;
FIG. 2 is a schematic view of a structure of a culture system in an embodiment of the present invention;
FIG. 3 is a schematic view of a cell culture plate and upper and lower layer array coils;
FIG. 4 is a schematic diagram of the electromagnetic field of plate wells on a cell culture plate;
in the figure, 1 is a case, 2 is an upper array coil, 3 is a cell culture plate, 4 is a lower array coil, 5 is a holder, and 6 is a power amplifier.
Detailed Description
The invention aims to provide a culture system for evaluating the biological electromagnetic effect of cells, which provides a constant-temperature and constant-humidity environment for the cells, reduces the influence of the external environment on the biological effect of the cells, and provides a basis for accurately evaluating the radiation effect of an electromagnetic field on the molecular level of the cells. The system comprises: the cell culture plate and the array coil are integrated in the cell culture box, and the magnetic field detection sensor is arranged, the principle of the cell culture plate is shown in figure 1, the signal generator generates specific waveform signals, the signals are amplified through the power amplifier and then drive the array coil to generate an alternating magnetic field, the alternating magnetic field acts on the cell culture plate, so that a uniform magnetic field environment is provided for cells in a plate hole, the actual magnetic field strength is measured through the alternating magnetic field sensor and fed back to the signal generator, closed-loop control is formed, and the magnetic field strength is controlled through adjusting waveform parameters output by the signal generator. The culture system takes the magnetic field as a variable under the condition of ensuring the normal temperature, humidity and PH value of cells to evaluate the influence of an electromagnetic field on the cell molecular level, ensures the stability and standardization of cell culture while reducing the influence of the external environment on the biological effect of the cells as much as possible, and provides a basis for accurately evaluating the radiation effect of the electromagnetic field on the cell molecular level.
Example (b):
referring to fig. 2, the culture system for cell bio-electromagnetic effect evaluation in the present embodiment includes: a signal generator, a power amplifier 6 and a cell culture box; the cell culture box comprises a box body 1, a bracket 5, a cell culture plate 3 and a magnetic field detection sensor, wherein the bracket 5, the cell culture plate 3 and the magnetic field detection sensor are arranged in the box body 1; the cell culture plate 3 is arranged on the bracket 5, and the cell culture plate 3 is provided with plate holes which are uniformly distributed and used for containing cell culture solution; cell culture plate's top is provided with one deck array coil 3, and the below is provided with one deck array coil 4, array coil 3 and array coil 4 are connected with power amplifier 6 electricity, power amplifier 6 is connected with signal generator electricity, the actual magnetic field intensity that magnetic field detection sensor is used for detecting in the cell culture case feeds back to signal generator, is used as signal generator to the regulation of the magnetic field intensity of production.
Specifically, the signal generator in this embodiment is configured to generate a specific waveform, which includes a plurality of waveforms such as a sine wave, a triangle wave, a square wave, and a pulse wave, set specific waveform parameters (frequency, magnitude, bias, phase, and the like), support a frequency range of 0Hz to 1MHz, and generate a corresponding alternating magnetic field (a sinusoidal alternating magnetic field, a triangle wave alternating magnetic field, a square wave alternating magnetic field, and a pulse wave alternating magnetic field) through a power amplifier control coil.
The power amplifier in the embodiment is used for amplifying the waveform of the signal generator to drive the coil, and when the type is specifically selected, the power amplifier with controllable gain can be selected, the harmonic distortion degree is lower than 1%, the driving power is more than 500w, the frequency response range is 0Hz-10khz, and the voltage range is 0-220 v.
The cell culture box can adopt a carbon dioxide culture box for standardized cell culture, and the environmental parameters are as follows: 37 ℃, 95% space humidity, 5% carbon dioxide gas; in the embodiment, a cell culture plate and an array coil are integrated and placed in a carbon dioxide incubator, and an alternating magnetic field sensor is arranged in the carbon dioxide incubator; then the array coil is connected with a power amplifier outside the incubator through a sealing port by using a cable, and the alternating magnetic field sensor is connected with a signal generator through a signal wire.
Cell culture plate's in this embodiment top and below respectively have one deck array coil, array coil comprises the circular coil of evenly arranging, the number of circular coil is the same with the pore plate number on the cell culture plate, the internal diameter of circular coil is the same with the internal diameter of pore plate, a pore plate is corresponding to each circular coil, promptly, guarantee that the center of pore plate is corresponding with circular coil's center when setting up, each pore plate all can be in between two upper and lower circular coils, thereby can improve the magnetic field homogeneity of cell in the pore plate, as shown in fig. 3 and 4. In addition, in order to further improve the uniformity of the magnetic field, the cell culture plate and the upper and lower array coils are arranged at equal intervals, and the interval between the upper and lower array coils is equal to the radius of the circular coil, so that the upper and lower array coils form a Helmholtz coil array, and according to the characteristics of the Helmholtz coil array, when the upper and lower coils are electrified in the same direction and with the same magnitude of current, a wider uniform magnetic field is generated near the midpoint of the common axis of the two coils. Therefore, the cell culture plate can be in a relatively uniform magnetic field environment.
In actual operation, magnetic field simulation can be performed through electromagnetic software, and the relative position of the array coil and the cell culture plate is finely adjusted according to a simulation result, so that the electromagnetic radiation environment borne by the cell plate is a uniform field.
It should be noted that the claimed solution includes but is not limited to the above embodiments, and those skilled in the art can make equivalent modifications/substitutions on the basis of the description of the above embodiments without departing from the protection scope of the present invention.
Claims (4)
1. A culture system for the evaluation of the biological electromagnetic effect of cells,
the method comprises the following steps: a signal generator, a power amplifier and a cell culture box; the cell culture box comprises a box body, a bracket, a cell culture plate and a magnetic field detection sensor, wherein the bracket, the cell culture plate and the magnetic field detection sensor are arranged in the box body; the cell culture plate is arranged on the bracket, and plate holes which are uniformly distributed and used for containing cell culture solution are arranged on the cell culture plate; a layer of array coil is respectively arranged above and below the cell culture plate; the signal generator is electrically connected with the power amplifier and is used for generating a plurality of driving currents with different waveforms and adjustable intensity and frequency; the power amplifier is electrically connected with the array coil and is used for amplifying the driving current so as to drive the array coil to generate an alternating magnetic field acting on the cell culture plate; the magnetic field detection sensor is electrically connected with the signal generator and used for detecting the actual magnetic field intensity in the cell culture box and feeding back the actual magnetic field intensity to the signal generator to form closed-loop control and serve as the adjustment of the signal generator to the generated magnetic field intensity.
2. A culture system for the evaluation of the biological electromagnetic effect of cells according to claim 1,
the array coil includes a plurality of circular coils of establishing ties, the number of circular coil with the number of diaphragm orifice equals, the internal diameter of circular coil with the internal diameter of diaphragm orifice equals.
3. A culture system for the evaluation of the biological electromagnetic effect of cells according to claim 2,
circular coils in the array coils above and below the cell culture plate correspond to plate holes one by one, and the center of each circular coil is aligned with the center of the corresponding plate hole.
4. A culture system for the evaluation of the biological electromagnetic effect of cells according to claim 3,
the distances between the array coils above and below the cell culture plate and the cell culture plate are equal, and the interval between the array coils above and below is equal to the radius of the circular coil.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202011042936.2A CN112111402A (en) | 2020-09-28 | 2020-09-28 | Culture system for cell biological electromagnetic effect evaluation |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202011042936.2A CN112111402A (en) | 2020-09-28 | 2020-09-28 | Culture system for cell biological electromagnetic effect evaluation |
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| CN112111402A true CN112111402A (en) | 2020-12-22 |
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| CN202011042936.2A Pending CN112111402A (en) | 2020-09-28 | 2020-09-28 | Culture system for cell biological electromagnetic effect evaluation |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114874907A (en) * | 2022-03-15 | 2022-08-09 | 华东师范大学 | System for detecting electromagnetic exposure cell biological effect in vitro |
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Application publication date: 20201222 |