CN112107503A

CN112107503A - Hair treatment method and application thereof

Info

Publication number: CN112107503A
Application number: CN202011171967.8A
Authority: CN
Inventors: 肖静; 李子源
Original assignee: Qilu University of Technology
Current assignee: Qilu University of Technology
Priority date: 2020-10-28
Filing date: 2020-10-28
Publication date: 2020-12-22

Abstract

The invention relates to the technical field of hair care and restoration, and particularly discloses a hair treatment method and application thereof.A damaged hair is sequentially soaked into a protein/amino acid composite aqueous solution and a TG enzyme solution; and then washing the damaged hair with water, and naturally airing. The invention has the beneficial effects that: the invention utilizes protein and amino acid to form protein network under the action of TG enzyme, and the protein network is crosslinked with protein groups exposed at the damaged part of the hair, the method is convenient to use and simple to operate; the repairing effect is good, the physical property and the damage resistance of the hair can be obviously improved, and the hair 'quasi-permanent bionic' maintenance is facilitated; has no chemical residue, and is green, healthy and pollution-free.

Description

Hair treatment method and application thereof

Technical Field

The invention relates to the technical field of hair care and restoration, in particular to a hair treatment method and application thereof.

Background

The hair is an important protein fiber, and the improvement of the life quality requires that the hair can be maintained healthily at a higher level on the basis of daily cleaning and washing. As early as 1988 Nicrout WKP et al used Croquat WKP quaternization to hydrolyze keratin for hair care, after which keratin, amino acids, etc. began to be used for hair care. However, most of the protein hair care methods are simple physical adsorption or weak crosslinking of chemical secondary bonds, and the repair has the advantages of certain moisture retention, smoothness, ultraviolet resistance and the like, but has some defects: the hair is easy to absorb water and swell, the effective time is short, and the hair surface adhesive protein is stripped to generate 'scurf' and the like. At present, the technical problems existing in the hair restoration are urgently needed to be solved through research, and a long-term effective hair nursing and restoring method is provided.

Disclosure of Invention

In order to solve the above problems, the present invention provides a hair treatment method and its application, which uses transglutaminase (TG enzyme) to cross-link with specific protein/amino acid to improve hair quality. According to the method, protein and amino acid form a protein net under the action of TG enzyme, and the protein net is crosslinked with protein groups exposed at the damaged part of the hair, so that the hair repairing effect is obviously improved.

In order to achieve the purpose, the invention provides the following scheme:

the invention provides a hair treatment method, which comprises the following steps: soaking damaged hair in protein and amino acid composite water solution for pretreatment, then putting into transglutaminase solution for repairing, taking out, cleaning hair, and air drying.

As a further optimization of the invention, the protein and amino acid composite aqueous solution contains 0.5 wt% -2 wt% of protein and 0.5 wt% -2 wt% of amino acid.

As a further optimization of the present invention, the protein is at least one of complex keratin, wheat protein and sericin.

As a further optimization of the invention, the compound keratin comprises the keratin with the molecular weight of 20KDa-30KDa and the keratin with the molecular weight of 0.8KDa-1.2KDa, the weight ratio is (0: 5) - (5: 0) in sequence, and the weight ratio is not 0 at the same time.

As a further optimization of the invention, the compound keratin comprises the keratin with the molecular weight of 20KDa-30KDa and the keratin with the molecular weight of 0.8KDa-1.2KDa, and the weight ratio is 3: 2 in sequence.

As a further optimization of the invention, the amino acid comprises at least one of L-alanine, L-lysine, L-serine and L-glycine.

As a further optimization of the invention, 4700U/g transglutaminase is present in the transglutaminase solution at a ratio of 0.5 wt% to 2 wt%.

As a further optimization of the invention, the pretreatment is to soak the damaged hair in a protein and amino acid composite aqueous solution according to the feed-liquid ratio of 0.1: 100g/ml, soak for 30min to 120min at the temperature of 30 ℃ to 60 ℃ and take out for later use.

As a further optimization of the invention, the repairing is that the damaged hair after pretreatment is soaked into transglutaminase solution according to the feed-liquid ratio of 0.1: 100g/ml, and the damaged hair is soaked for 30-120 min under the conditions that the temperature is 30-60 ℃ and the pH is 6.0-8.0.

As a further optimization of the invention, the cleaning is water washing for 3-5 times, and each time is 3min-5 min.

The invention also provides the use of the hair treatment method for repairing damaged hair, including pet hair as well as human hair.

The invention discloses the following technical effects:

1. the present invention adopts lower keratin concentration than traditional method and is not easy to form protein chip.

2. The invention adopts the combination of TG enzyme, specific protein and amino acid, the formed amido bonds are increased, the cross-linking effect is obvious, the crystallinity is improved, the percentage of a crystalline region in the whole fiber is increased, the effect is stable, and the invention is a 'quasi-permanent' bionic repair. The method is convenient to use and simple to operate; the hair restoring agent has good restoring effect, can obviously improve the physical property and the damage resistance of the hair, has no chemical residue, and is green, healthy and pollution-free.

3. The hair conditioner has obvious maintenance effect, obvious repair effect on damaged scales (protein exposed) of the hair, reduced alkali solubility of the repaired hair, improved breaking stress, reduced water swelling rate and improved physical and chemical stability; the repaired hair is smoother than the surface of the damaged hair, and the fluctuation of hair scales is smooth. The hair is bright, elastic and tough, the straight hair is more flexible, and the curling degree can be better maintained by curling.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings needed to be used in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings without inventive exercise.

FIG. 1 is an electron microscope observation of the surface of damaged human hair before repair and the surface of repaired hair in example 2, wherein A is damaged human hair before repair and B is repaired hair;

FIG. 2 is a graph comparing the swelling of damaged human hair before and after imbibition of water, before repair in example 2;

FIG. 3 is a graph comparing the swelling before and after water absorption of the repaired hair of example 2;

fig. 4 is a comparison graph of the hair which is just permed and curled, the permed and curled damaged hair with the curling degree lost after washing and protecting by the common method and the repaired hair in example 7, wherein c is 1 group of the permed and curled damaged hair with the curling degree lost after washing and protecting for a certain number of times by the common method, d is 2 groups of the repaired hair with the curling degree lost by the treating method of the embodiment, and e is the permed and curled hair before washing and protecting.

Detailed Description

Reference will now be made in detail to various exemplary embodiments of the invention, the detailed description should not be construed as limiting the invention but as a more detailed description of certain aspects, features and embodiments of the invention.

It is to be understood that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. Further, for numerical ranges in this disclosure, it is understood that each intervening value, between the upper and lower limit of that range, is also specifically disclosed. Every smaller range between any stated value or intervening value in a stated range and any other stated or intervening value in a stated range is encompassed within the invention. The upper and lower limits of these smaller ranges may independently be included or excluded in the range.

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Although only preferred methods and materials are described herein, any methods and materials similar or equivalent to those described herein can be used in the practice or testing of the present invention. All documents mentioned in this specification are incorporated by reference herein for the purpose of disclosing and describing the methods and/or materials associated with the documents. In case of conflict with any incorporated document, the present specification will control.

It will be apparent to those skilled in the art that various modifications and variations can be made in the specific embodiments of the present disclosure without departing from the scope or spirit of the disclosure. Other embodiments will be apparent to those skilled in the art from consideration of the specification. The description and examples are intended to be illustrative only.

As used herein, the terms "comprising," "including," "having," "containing," and the like are open-ended terms that mean including, but not limited to.

The protein, amino acid, and TG enzyme solutions described in the examples of the present invention were all commercially available.

The damaged human hair selected for examples 2-6 was a sample from the same batch.

Example 1

(1) In the embodiment, the concentration of the compound keratin in the protein and amino acid compound aqueous solution is 0.5 wt%, the concentration of the L-alanine is 1 wt%, the damaged pet dog hair is soaked into the compound aqueous solution according to the proportion that 100mL of the solution is used for every 0.1g of damaged hair, and the hair is soaked for 120min at the temperature of 40 ℃ and taken out, so that the pretreated pet dog hair is obtained for later use;

(2) soaking the pretreated pet dog hair obtained in (1) into 100mL of 4700U/g TG enzyme solution with concentration of 0.5 wt% according to the proportion of 100mL of solution used for every 0.1g of damaged hair, and soaking for 120min at 40 ℃ and pH7.0 for repairing;

(3) and (3) washing the hair of the pet dog subjected to the pretreatment of the repairing in the step (2) for 5 times, each time for 3min, paving the hair in a shady place, and naturally drying the hair to obtain the hair of the repaired pet dog.

The compound keratin in the embodiment is the mixture of keratin with the molecular weight of 20KDa-30KDa and keratin with the molecular weight of 0.8-1.2KDa according to the weight ratio of 1: 1.

To test the effectiveness of the use of a product according to one embodiment of the present invention, we performed alkali solubility and breaking stress tests on pet dog hair restoration. The results are shown in Table 1.

TABLE 1

The hair of the repaired pet dog is compared with the hair of the damaged pet dog, and the alkali solubility of the hair of the repaired pet dog is reduced to 9.98 percent from 19.35 percent, and the breaking stress is improved to 1.64N from 0.73N.

Example 2

(1) In the embodiment, the composite keratin concentration of the protein and amino acid composite aqueous solution is 1 wt%, the L-lysine concentration is 1 wt%, and the damaged human hair is soaked into the composite aqueous solution according to the proportion that 100mL of solution is used for every 0.1g of damaged hair, and is soaked for 90min at the temperature of 30 ℃ and taken out, so that the pretreated hair is obtained for later use;

(2) soaking the pretreated hair obtained in (1) into 100mL of 4700U/g TG enzyme solution with concentration of 2 wt% at 40 deg.C and pH of 7.0 for 120min according to the ratio of 100mL of solution for each 0.1g of damaged hair, and repairing;

(3) and (3) washing the pretreated hairline repaired in the step (2) for 3 times, each time for 3min, paving the hairline in a shady and cool place, and naturally drying the hairline to obtain the repaired hairline.

The compound keratin in the embodiment is the mixture of the keratin with the molecular weight of 20-30KDa and the keratin with the molecular weight of 0.8-1.2KDa according to the weight ratio of 3: 2.

In order to test the use effect of a product according to the present invention, we performed alkali solubility and breaking stress tests on the damaged human hair before repair and the repaired hair of this example. The results are shown in Table 2.

TABLE 2

Sample name	Alkali solubility (unit:%)	Breaking stress (unit: N)
			Damaged human hair	16.14±0.45	0.83±0.13
Example 2 restoration of hair	4.92±0.16	2.04±0.37

Compared with the damaged human hair, the alkali solubility of the repaired hair is reduced to 4.92% from 16.14%, and the breaking stress is improved to 2.04N from 0.83N.

The damaged human hair and the repaired hair before the repair in this example were observed by an electron microscope, and fig. 1 was obtained. The comparison before and after the restoration in fig. 1 shows that the treatment method of the invention has obvious restoration effect on the damaged scales (protein exposed) parts of the hair, the surface of the restored hair is smoother than that of the damaged hair, and the fluctuation of the hair scales is smooth.

Soaking the damaged hair and the repaired hair before repairing in water, performing water absorption treatment, observing the comparison before and after water absorption of the damaged hair to obtain a figure 2, and observing the comparison before and after water absorption of the repaired hair to obtain a figure 3. The comparison between figure 2 and figure 3 shows that the water swelling rate of the hair repaired by the treatment method of the invention is obviously reduced.

Example 3

(1) In the embodiment, the concentration of the compound keratin in the protein and amino acid compound aqueous solution is 1.5 wt%, the concentration of the L-serine is 2 wt%, the damaged human body hair is soaked into the compound aqueous solution according to the proportion that 100mL of solution is used for every 0.1g of damaged hair, and the damaged human body hair is soaked for 30min at the temperature of 60 ℃ and taken out, so that the pretreated hair is obtained for later use;

(2) soaking the pretreated hair obtained in (1) into 100mL of 4700U/g TG enzyme solution with concentration of 2 wt% at 30 deg.C and pH of 6.0 for 30min according to the ratio of 100mL of solution for each 0.1g of damaged hair, and repairing;

(3) and (3) washing the pretreated hairline repaired in the step (2) for 4 times, each time for 4min, paving the hairline in a shady and cool place, and naturally drying the hairline to obtain the repaired hairline.

The compound keratin in the embodiment is the mixture of the keratin with the molecular weight of 20-30KDa and the keratin with the molecular weight of 0.8-1.2KDa according to the weight ratio of 1: 5.

In order to test the use effect of a product according to the present invention, we performed alkali solubility and breaking stress tests on the damaged human hair before repair and the repaired hair of this example. The results are shown in Table 3.

TABLE 3

Sample name	Alkali solubility (unit:%)	Breaking stress (unit: N)
			Damaged human hair	16.14±0.45	0.83±0.13
Example 3 restoration of hair	5.51±0.13	1.72±0.28

The repair hair is compared with the damaged human hair, and the alkali solubility is reduced to 5.51% from 16.14%, and the breaking stress is improved to 1.72N from 0.83N.

Example 4

(1) In the embodiment, the composite keratin concentration of the protein and amino acid composite aqueous solution is 2 wt%, the L-glycine concentration is 0.5 wt%, the damaged human body hair is soaked into the composite aqueous solution according to the proportion that 100mL of solution is used for every 0.1g of damaged hair, and the damaged human body hair is soaked for 30min at the temperature of 40 ℃ and taken out, so that the pretreated hair is obtained for later use;

(2) soaking the pretreated hair obtained in (1) into 100mL of 4700U/g TG enzyme solution with concentration of 1 wt% at 60 deg.C and pH of 8.0 for 90min according to the proportion of 100mL of solution used for every 0.1g of damaged hair, and repairing;

(3) and (3) washing the pretreated hairline repaired in the step (2) for 5 times, each time for 5min, paving the hairline in a shady and cool place, and naturally drying the hairline to obtain the repaired hairline.

The compound keratin in the embodiment is the mixture of the keratin with the molecular weight of 20-30KDa and the keratin with the molecular weight of 0.8-1.2KDa according to the weight ratio of 5: 1.

In order to test the use effect of a product according to the present invention, we performed alkali solubility and breaking stress tests on the damaged human hair before repair and the repaired hair of this example. The results are shown in Table 4.

TABLE 4

Sample name	Alkali solubility (unit:%)	Breaking stress (unit: N)
			Damaged human hair	16.14±0.45	0.83±0.13
Example 4 repair of hair	6.03±0.12	1.87±0.22

Compared with the damaged human hair, the alkali solubility of the repaired hair is reduced to 6.03 percent from 16.14 percent, and the breaking stress is improved to 1.87N from 0.83N.

Example 5

(1) In the embodiment, the sericin concentration of the protein and amino acid composite aqueous solution is 2 wt%, the L-glycine concentration is 0.5 wt%, damaged human body hair is soaked into the composite aqueous solution according to the proportion that 100mL of solution is used for every 0.1g of damaged hair, and the damaged human body hair is soaked for 30min at the temperature of 40 ℃ and taken out, so that the pretreated hair is obtained for later use;

(3) and (3) washing the pretreated hairline repaired in the step (2) for 5 times, and paving the hair line in a shady and cool place for naturally drying the hair line for 5min each time to obtain the repaired hairline.

In order to test the use effect of a product according to the present invention, we performed alkali solubility and breaking stress tests on the damaged human hair before repair and the repaired hair of this example. The results are shown in Table 5.

TABLE 5

Sample name	Alkali solubility (unit:%)	Breaking stress (unit: N)
			Damaged human hair	16.14±0.45	0.83±0.13
Example 5 repair of hair	6.84±0.22	1.54±0.18

The repair hair is compared with the damaged human hair, and the alkali solubility is reduced to 6.84% from 16.14%, and the breaking stress is improved to 1.54N from 0.83N.

Example 6

(1) In the embodiment, the wheat protein concentration in the protein and amino acid composite aqueous solution is 2 wt%, the L-glycine concentration is 0.5 wt%, the damaged human body hairline is soaked in the composite aqueous solution according to the proportion that 100mL of solution is used for every 0.1g of damaged hairline, and the damaged human body hairline is soaked for 30min at the temperature of 40 ℃ and taken out, so that the pretreated hairline is obtained for later use;

In order to test the use effect of a product according to the present invention, we performed alkali solubility and breaking stress tests on the damaged human hair before repair and the repaired hair of this example. The results are shown in Table 6.

TABLE 6

Sample name	Alkali solubility (unit:%)	Breaking stress (unit: N)
			Damaged human hair	16.14±0.45	0.83±0.13
Example 6 restoration of hair	9.86±0.25	1.61±0.13

The repair hair is compared with the damaged human hair, and the alkali solubility is reduced to 9.86% from 16.14%, and the breaking stress is improved to 1.61N from 0.83N.

Example 7

In the embodiment, the hot hair curlers are selected as samples to be subjected to washing and protecting experiments, wherein 1 group of the hot hair curlers are washed and protected by a common method, and 2 groups of the hot hair curlers are washed and protected by the treatment method. The 2-group washing and protecting method comprises the following steps:

(1) in the embodiment, the composite keratin concentration of the protein and amino acid composite aqueous solution is 1 wt%, the L-lysine concentration is 0.5 wt%, the damaged human body hair is soaked into the composite aqueous solution according to the proportion that 100mL of solution is used for every 0.1g of damaged hair, and the damaged human body hair is soaked for 30min at the temperature of 40 ℃ and taken out, so that the pretreated hair is obtained for later use;

(2) soaking the pretreated hair obtained in (1) into 100mL of 4700U/g TG enzyme solution with concentration of 1 wt% at 50 deg.C and pH of 8.0 for 90min according to the ratio of 100mL of solution for each 0.1g of damaged hair, and repairing;

The hair of the perm and curls, 1 group of perm and curly damaged hair with the curliness lost after being washed and protected for a plurality of times by the common method and 2 groups of repaired hair washed and protected for the same number of times by the processing method of the embodiment are observed and compared to obtain a graph 4. According to observation, the treatment method of the invention can well wash, protect and repair the curly hair, and compared with the result that the curly hair disappears caused by the common washing and protecting method, the curly hair washed and protected by the method of the embodiment for the same times can still keep the natural curly hair.

Comparative example 1

In the prior art, high-concentration keratin is used for repairing, and 0.8-1.2KDa molecular weight keratin compound liquid is used for repairing, wherein the concentration is 10 wt%.

(1) Soaking the damaged hair into the keratin complex solution at 30 deg.C for 90min at a ratio of 100mL per 0.1g damaged hair.

(2) And (3) washing the treated damaged human hair with water for 3 times, 3min each time, laying flat, placing in a shade, and naturally drying to obtain the repaired hair.

To compare the effectiveness of the use of a product according to the invention, we compared the alkali solubility and the breaking stress of comparative example 1 with example 2. The results are shown in Table 7.

TABLE 7

Sample name	Alkali solubility (unit:%)	Breaking stress (unit: N)
			Damaged human hair	16.14±0.45	0.83±0.13
Comparative example 1 hair restoration	12.19±0.16	1.24±0.12
			Example 2 restoration of hair	4.92±0.16	2.04±0.37

Comparative example 1 the alkali solubility of the repaired hair was reduced from 16.14% to 12.19%, and the breaking stress was increased from 0.83N to 1.24N; while the alkali solubility of the repaired hair of the example 2 is reduced to 4.92% from 16.14%, and the breaking stress is improved to 2.04N from 0.83N. The effect of the repair by the combined repair process of TG enzyme-keratin/amino acid is better than that of the repair by the traditional process.

Comparative example 2

(1) The comparative example mainly treats the damaged human hair, the concentration of 0.8-1.2KDa protein aqueous solution is 5 wt%, the damaged human hair is soaked into the protein aqueous solution according to the proportion that 100mL of solution is used for every 0.1g of damaged hair, and the damaged human hair is soaked for 60min at the temperature of 40 ℃ and taken out, so that the pretreated hair is obtained for later use;

(2) soaking the pretreated hair obtained in (1) into 100mL of 4700U/g TG enzyme solution with concentration of 1 wt% at 30 deg.C and pH of 7.0 for 30min according to the proportion of 100mL of solution used for each 0.1g of damaged hair, and repairing;

To compare the effectiveness of the use of a product according to one embodiment of the invention, we compared the alkali solubility and the breaking stress of comparative example 2 with example 2. The results are shown in Table 8.

TABLE 8

Sample name	Alkali solubility (unit:%)	Breaking stress (unit: N)
			Damaged human hair	16.14±0.45	0.83±0.13
Comparative example 2 repair hair	8.19±0.21	1.54±0.31
			Example 2 restoration of hair	4.92±0.16	2.04±0.37

Comparative example 2 the alkali solubility of the repaired hair was reduced from 16.14% to 8.19%, and the breaking stress was increased from 0.83N to 1.54N; while the alkali solubility of the repaired hair of the example 2 is reduced to 4.92% from 16.14%, and the breaking stress is improved to 2.04N from 0.83N. The effect of the combined repair of the protein/amino acid compound liquid and the TG enzyme is better than that of the simple combined repair of the keratin and the TG enzyme.

The above-described embodiments are merely illustrative of the preferred embodiments of the present invention, and do not limit the scope of the present invention, and various modifications and improvements of the technical solutions of the present invention can be made by those skilled in the art without departing from the spirit of the present invention, and the technical solutions of the present invention are within the scope of the present invention defined by the claims.

Claims

1. A method of hair treatment comprising the steps of: soaking damaged hair in protein and amino acid composite water solution for pretreatment, then putting into transglutaminase solution for repairing, taking out, cleaning hair, and air drying.

2. The hair treatment method according to claim 1, wherein the protein-amino acid complex aqueous solution contains 0.5 to 2 wt% of protein and 0.5 to 2 wt% of amino acid.

3. The hair treatment method according to claim 2, wherein the protein is at least one of complex keratin, wheat protein, and sericin.

4. The hair treatment method according to claim 3, wherein the complex keratin comprises 20kDa-30kDa keratin and 0.8kDa-1.2kDa keratin in the weight ratio of (0: 5) - (5: 0) in order, and is not 0 at the same time.

5. The hair treatment method according to claim 1, wherein the amino acid comprises at least one of L-alanine, L-lysine, L-serine, and L-glycine.

6. The hair treatment method according to claim 1, characterized in that 4700U/g transglutaminase is contained in the transglutaminase solution at a ratio of 0.5 wt% to 2 wt%.

7. The hair treatment method according to claim 1, wherein the pretreatment is to soak damaged hair in a protein and amino acid composite aqueous solution according to a feed-liquid ratio of 0.1: 100g/ml, soak for 30min to 120min at 30 ℃ to 60 ℃, and take out for later use.

8. The hair treatment method according to claim 1, wherein the repairing is carried out by soaking the pre-treated damaged hair in a transglutaminase solution at a feed-to-solution ratio of 0.1: 100g/ml for 30min to 120min at a temperature of 30 ℃ to 60 ℃ and a pH of 6.0 to 8.0.

9. The hair treatment method according to claim 1, wherein the cleaning is water washing 3 to 5 times for 3min to 5min each.

10. Use of a hair treatment process according to any of claims 1 to 9 for repairing damaged hair, wherein said damaged hair comprises pet hair as well as human hair.