CN112080421B - Ultrahigh-flux full-automatic pathogen nucleic acid detection system - Google Patents
Ultrahigh-flux full-automatic pathogen nucleic acid detection system Download PDFInfo
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Abstract
The application relates to an ultra-high flux full-automatic pathogen nucleic acid detection system, which comprises an extraction mechanism, a transmission window, a configuration mechanism and a detection mechanism, wherein the extraction mechanism comprises a plurality of extraction areas which are arranged side by side, and each extraction area is respectively provided with an extraction tool for extracting high flux nucleic acid; the first mechanical arms are used for automatically executing the operation of extracting nucleic acid from the sample under the drive of the first mechanical arms; a second mechanical arm; the configuration mechanism comprises a third mechanical arm, a fourth mechanical arm and a configuration tool, wherein the third mechanical arm is used for transferring samples from the transmission window to the configuration tool, and the fourth mechanical arm is used for configuring the configuration tool and carrying out samples between the configuration tools; and the detection mechanism is used for detecting nucleic acid on the sample processed by the configuration mechanism. The application can fully automatically detect nucleic acid, and has the advantages of convenient operation and time saving.
Description
Technical Field
The application relates to the technical field of nucleic acid detection, in particular to an ultrahigh-flux full-automatic pathogen nucleic acid detection system.
Background
In order to effectively prevent and control the input epidemic situation, people need to be screened. Large-scale rapid screening is currently the most effective means for preventing and controlling highly pathogenic infectious diseases, "importable" and "asymptomatic infectors". The method comprises the steps of detecting whether the human body is infected by the virus nucleic acid in a respiratory tract specimen, blood or excrement of the human body, and once the detection shows that the nucleic acid is positive, proving that the virus exists in the human body, and simultaneously, breeding the human body in a respiratory tract system after the human body is infected by the highly pathogenic infectious disease, so that the detection of the nucleic acid can be used as a gold standard for the accurate diagnosis of the highly pathogenic infectious disease infection by conveniently detecting the virus nucleic acid in sputum and nasopharyngeal swab to judge whether the human body is infected by the virus.
When a human body is sampled, the sampled sample is required to be extracted and detected, a standard PCR laboratory is required for conventional nucleic acid detection, and the steps of pretreatment of the sample, nucleic acid extraction, preparation of a detection system, nucleic acid amplification, detection and the like are finished through multi-step instrument use and manual operation by a professional nucleic acid detector, so that the problems of long time consumption and complex operation exist.
Disclosure of Invention
Therefore, the technical problem to be solved by the application is to overcome the defects of long time consumption and complicated operation in the prior art, thereby providing the ultrahigh-flux full-automatic pathogen nucleic acid detection system.
The application aims to overcome the defect of complicated operation in the prior art, thereby providing an ultrahigh-flux full-automatic pathogen nucleic acid detection using method.
In order to achieve the above purpose, the application adopts the following technical scheme: comprises an extraction mechanism, a transmission window, a configuration mechanism and a detection mechanism,
an extraction mechanism comprising a plurality of extraction areas side by side, each of the extraction areas being respectively configured with extraction means for performing high-throughput nucleic acid extraction; the first mechanical arms are used for automatically executing the operation of extracting nucleic acid from the sample under the drive of the first mechanical arms; a second mechanical arm for transferring the sample extracted from the nucleic acid to a transfer window;
the configuration mechanism is connected with the extraction mechanism through the transmission window and comprises a third mechanical arm, a fourth mechanical arm and a configuration tool, wherein the third mechanical arm is used for transferring samples from the transmission window to the configuration tool, the fourth mechanical arm is used for configuring the configuration tool and transferring the samples between the configuration tools, and the configuration tool is used for automatically performing operations such as film sealing, vibration, centrifugation and the like;
and the fourth mechanical arm is also used for transferring the sample processed by the configuration mechanism into the detection mechanism.
Further, the extraction region includes:
the device comprises a first consumable supply drawer, wherein a plurality of rows of deep hole plates are arranged on the first consumable supply drawer along the length direction of the first consumable supply drawer, a plurality of deep hole plates are arranged on each row of deep hole plates, the extraction tool comprises a magnetic rod magnetic sleeve assembly arranged on a first mechanical arm, and the magnetic rod magnetic sleeve assembly is driven by the first mechanical arm to conduct nucleic acid extraction operation.
Further, the magnetic rod and magnetic sleeve assembly comprises a magnetic rod loading module and a magnetic sleeve loading module, wherein the magnetic rod loading module and the magnetic sleeve loading module are coaxially arranged, and the magnetic rod loading module are driven to move by the first mechanical arm.
Further, the configuration mechanism further comprises a second consumable supply drawer, the configuration tool comprises a plurality of low-temperature modules, a film sealing module, an oscillation module and a centrifugal module, a plurality of pore plates are arranged on the second consumable supply drawer, and the third mechanical arm is used for transferring samples in the deep pore plates located in the transfer window into the pore plates.
Further, the detection mechanism comprises a large shading box which is arranged in a hollow mode, an opening is formed in the side wall of the large shading box, and a detector for detecting a sample is arranged in the large shading box.
Further, the transfer window comprises a hollow transfer window box body, sealing windows are formed in the top side surfaces of two ends of the transfer window box body, movable sealing doors are arranged at the sealing windows, the sealing doors seal the sealing windows, deep-hole plate brackets are arranged in the transfer window box body, and fifth mechanical arms for driving the deep-hole plate brackets to move towards the length direction of the transfer window are arranged on the bottom sides of the deep-hole plate brackets.
Further, the method further comprises the following steps: the code scanning input module is used for collecting information of the sample before extracting nucleic acid from the sample.
Furthermore, a sample loading module is further arranged at the deep hole plate, the sample is positioned in the sample loading module, and the sample in the sample loading module is transferred into the deep hole plate through the second mechanical arm.
The ultra-high throughput fully automatic pathogen nucleic acid detection method comprises the following steps:
driving an extraction tool of each extraction region in the plurality of side-by-side extraction regions to perform an operation of extracting nucleic acid from a sample by a first mechanical arm;
transferring the sample extracted from the nucleic acid to a transfer window by using a second mechanical arm;
transferring a sample from the transfer window to the configuration tool using a third robotic arm;
the fourth mechanical arm is utilized to configure the configuration tool, and then the configuration tool is controlled to automatically perform operations such as film sealing, vibration, centrifugation and the like;
transferring the sample processed by the configuration mechanism to the detection mechanism by using the fourth mechanical arm;
the detection mechanism performs temperature control, fluorescence imaging and result analysis on the processed sample to derive a detection result.
Further, before the operation of extracting the nucleic acid from the sample by the first robot arm driving the extracting tool of each of the plurality of side-by-side extracting regions, the method further comprises:
placing a sample into a sample loading module;
the sample loading module performs code scanning information input through the code scanning input module;
and placing the sample loading module with the recorded information at a deep hole plate of the extraction area, and releasing the sample into the deep hole plate through the positive pressure sample loading module of the second mechanical arm.
The technical scheme of the application has the following advantages:
1. the application provides an ultrahigh-flux full-automatic pathogen nucleic acid detection system, which comprises an extraction mechanism, a transmission window, a configuration mechanism and a detection mechanism, wherein a detection person puts a nucleic acid sample to be detected into an extraction tool in the extraction mechanism when in use, under the drive of a first mechanical arm, the operations of virus splitting, nucleic acid releasing, nucleic acid combining and nucleic acid purifying are automatically executed, and nucleic acid is extracted from the sample, after the nucleic acid is extracted, a second mechanical arm starts to work, the extracted nucleic acid sample is transferred into the transmission window, the sample with the extracted nucleic acid is moved to the configuration mechanism under the action of the transmission window, a third mechanical arm starts to work, the sample with the extracted nucleic acid is transferred to the configuration tool, and under the action of a fourth mechanical arm, the sample is transferred into the detection mechanism again after the sample is configured, and temperature control, fluorescence imaging and result analysis are executed on the sample in the detection mechanism, so that the high-flux nucleic acid extraction and detection treatment are realized in the whole nucleic acid detection process, the sample is not required to be more convenient and more rapid to operate.
2. The ultra-high flux full-automatic pathogen nucleic acid detection system provided by the application realizes the parallel processing of a multi-sample nucleic acid detection flow through the cooperation of precise machinery and the use of a high flux module, and comprises the parallel pretreatment, nucleic acid extraction, system configuration, nucleic acid amplification detection and the like, so that the single-processing sample quantity is greatly improved, and the nucleic acid detection efficiency is greatly improved.
3. The ultrahigh-flux full-automatic pathogen nucleic acid detection system provided by the application comprises the code scanning input module, wherein the code scanning input module is used for collecting information of a sample before extracting nucleic acid from the sample, and the code scanning input module is used for inputting information of the sample in advance before extracting, configuring and detecting the sample, so that after the sample is detected, the result and the information of the sample can be well corresponding, the information of the sample is not required to be tidied, and the working efficiency is improved.
4. According to the ultrahigh-flux full-automatic pathogen nucleic acid detection method provided by the application, a nucleic acid piece is extracted, a sample is placed into a sample loading module, information is input through a code scanning input module, the sample loading module with the input information is placed into a deep hole plate, a second mechanical arm punctures the sample loading module by positive pressure, the sample in the sample loading module is released into the deep hole plate, and under the action of a first mechanical arm, a magnetic rod magnetic sleeve assembly is driven to move, so that virus splitting, nucleic acid releasing, nucleic acid combining and nucleic acid purifying are realized through operations such as vibration mixing, magnetic bead transferring, washing and eluting, the extraction of nucleic acid is completed, after the nucleic acid is extracted, the second mechanical arm grabs the deep hole plate with the extracted nucleic acid, the deep hole plate is moved to a transfer window, the deep hole plate is moved to a configuration mechanism through the transfer window, under the action of a third mechanical arm at the configuration mechanism, the sample in the deep hole plate is transferred into the hole plate, the hole plate is sequentially moved into a low-temperature module, a sealing membrane module, an oscillating module and a centrifugal module through a fourth mechanical arm to be processed, and the fourth mechanical arm is moved to a detection mechanism after the processing is completed, the temperature control, the sample is controlled, and the detection result is imaged, and the detection result is obtained. The ultra-high flux parallel sample processing is realized in the whole detection method, the whole process is automatic, the transfer of the sample is not needed by manpower, the time and the labor are saved, and the sample processing efficiency is greatly improved.
Drawings
In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings that are needed in the description of the embodiments or the prior art will be briefly described, and it is obvious that the drawings in the description below are some embodiments of the present application, and other drawings can be obtained according to the drawings without inventive effort for a person skilled in the art.
FIG. 1 is a schematic diagram of the overall structure of an ultra-high throughput fully automated pathogen nucleic acid detection system provided in a first embodiment of the application;
FIG. 2 is a schematic view of the extraction mechanism shown in FIG. 1;
FIG. 3 is a schematic view of the first supply drawer of FIG. 2;
FIG. 4 is a schematic view of the extraction mechanism shown in FIG. 2;
fig. 5 is a schematic view of the refuse station of fig. 2;
FIG. 6 is a schematic diagram of the transmission window shown in FIG. 2;
FIG. 7 is a schematic view illustrating an internal structure of the transfer window shown in FIG. 6;
FIG. 8 is a schematic diagram of the configuration and detection mechanisms shown in FIG. 1;
FIG. 9 is a schematic diagram of the detection mechanism shown in FIG. 8;
fig. 10 is a step diagram of the ultra-high throughput fully automatic pathogen nucleic acid detection method provided by the application.
Reference numerals illustrate:
1. an extraction mechanism; 11. an extraction tool; 111. a magnetic rod and magnetic sleeve assembly; 1111. a magnetic rod loading module; 1112. the magnetic sleeve loading module; 12. a first mechanical arm; 121. a magnetic sleeve mechanical arm; 122. a magnetic rod mechanical arm; 13. a second mechanical arm; 14. a first consumable supply drawer; 15. a deep well plate; 2. a transfer window; 21. a transfer window box; 22. sealing the window; 23. sealing the door; 24. a deep hole plate bracket; 25. a fifth mechanical arm; 3. a configuration mechanism; 31. a third mechanical arm; 32. a fourth mechanical arm; 33. a configuration tool; 331. a low temperature module; 332. a film sealing module; 333. an oscillation module; 334. a centrifugal module; 34. a second consumable supply drawer; 35. an orifice plate; 4. a detection mechanism; 41. a large light shielding box; 42. a detector; 5. the code scanning input module; 6. a sample loading module; 7. a waste station; 71. a waste station aisle; 72. a garbage station bracket; 8. an ultraviolet sterilizing lamp; 9. a limit bar; 10. a fixed block; 20. a reference plate.
Detailed Description
The following description of the embodiments of the present application will be made apparent and fully in view of the accompanying drawings, in which some, but not all embodiments of the application are shown. All other embodiments, which can be made by those skilled in the art based on the embodiments of the application without making any inventive effort, are intended to be within the scope of the application.
In the description of the present application, it should be noted that the directions or positional relationships indicated by the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc. are based on the directions or positional relationships shown in the drawings, are merely for convenience of describing the present application and simplifying the description, and do not indicate or imply that the devices or elements referred to must have a specific orientation, be configured and operated in a specific orientation, and thus should not be construed as limiting the present application. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
In the description of the present application, it should be noted that, unless explicitly specified and limited otherwise, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be either fixedly connected, detachably connected, or integrally connected, for example; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communication between two elements. The specific meaning of the above terms in the present application will be understood in specific cases by those of ordinary skill in the art.
In addition, the technical features of the different embodiments of the present application described below may be combined with each other as long as they do not collide with each other.
Example 1
The ultra-high throughput full-automatic pathogen nucleic acid detection system provided in this embodiment, as shown in fig. 1 and 2, includes an extraction mechanism 1, a transmission window 2, a configuration mechanism 3 and a detection mechanism 4, wherein the extraction mechanism 1 is firstly placed with a sample when detecting the sample, the sample is subjected to high throughput nucleic acid extraction under the action of the extraction mechanism 1, the sample with the extracted nucleic acid is moved to the configuration mechanism 3 through the transmission window 2, and automatic membrane sealing, vibration, centrifugation and other operations are performed in the configuration mechanism 3, so that the sample is subjected to multi-step configuration, the configured sample is moved to the detection mechanism 4, and the sample is subjected to automatic temperature control, fluorescence imaging and result analysis under the action of the detection mechanism, and finally the detection result is derived. Here, the extraction mechanism 1 includes three extraction regions arranged side by side, each of which is provided with an extraction tool 11 for performing high-throughput nucleic acid extraction, and the nucleic acid extraction is performed on a sample by the extraction tools 11. A first robot arm 12 and a second robot arm 13 are also arranged in the extraction area, the second robot arm 13 being arranged as a plate grabbing robot arm. When extracting nucleic acid from a sample, the first mechanical arm 12 drives the extracting tool 11 to automatically perform the operation of extracting nucleic acid from the sample, after extracting nucleic acid from the sample, the sample with the extracted nucleic acid is transferred to the transmission window 2 under the drive of the second mechanical arm 13, and the sample with the extracted nucleic acid is moved to the configuration mechanism 3 under the action of the transmission window 2 to automatically perform the configuration operations such as film sealing, vibration, centrifugation and the like on the sample.
As shown in fig. 1 and 8, the configuration mechanism 3 is connected with the extraction mechanism 1 through the transfer window 2, the configuration mechanism 3 comprises a third mechanical arm 31, a fourth mechanical arm 32 and a configuration tool 33, when the sample with extracted nucleic acid moves to the configuration mechanism 3 through the transfer window 2, the third mechanical arm 31 drives the sample to be transferred to the configuration tool 33 for processing, at the moment, the fourth mechanical arm 32 can drive the sample to be transferred between different configuration tools 33 for configuration, after the sample is configured, the sample is moved to the detection mechanism 4 through the fourth mechanical arm 32, temperature control, fluorescence imaging and result analysis are performed on the sample in the detection mechanism 4, so that a detection result is obtained, and in the whole nucleic acid detection process, the whole process of nucleic acid extraction, system configuration and nucleic acid detection are processed in parallel, and full-automatic high-throughput nucleic acid extraction and detection processing is realized, the sample is not required to be transferred by manpower, and the operation is more convenient and rapid.
As shown in fig. 2 and 3, in this, the extraction area includes a first consumable supply drawer 14, the first mechanical arm 12 and the second mechanical arm 13 are located above the first consumable supply drawer 14, six rows of deep hole plates 15 are provided on the first consumable supply drawer 14, the six rows of deep hole plates 15 are provided along the length direction of the first consumable supply drawer 14, the six rows of deep hole plates 15 are uniformly arranged on the first consumable supply drawer 14, four deep hole plates 15 are provided in each row, and four deep hole plates 15 in each row are provided along the width direction of the first consumable supply drawer 14 and uniformly arranged on the first consumable supply drawer 14. All be equipped with spacing 9 between two rows of deep hole boards 15 adjacent, spacing 9 can dismantle and connect on first consumptive material supply drawer 14, spacing 9 contradicts with deep hole board 15, integrated into one piece has a plurality of fixed blocks 10 on the lateral wall of spacing 9, a plurality of fixed blocks 10 are located respectively between four deep hole boards 15 of every row and contradict with deep hole board 15 to inject the position of deep hole board 15 through spacing 9, when making carrying out nucleic acid extraction to the sample in the deep hole board 15, can be more accurate.
As shown in fig. 2 and 4, the extracting tool 11 includes a magnetic rod magnetic sleeve assembly 111 disposed on the first mechanical arm 12, and the magnetic rod magnetic sleeve assembly 111 performs a nucleic acid extracting operation under the driving of the first mechanical arm 12. The magnetic rod and magnetic sleeve assembly 111 comprises a magnetic rod loading module 1111 and a magnetic sleeve loading module 1112, wherein the magnetic rod loading module 1111 is positioned above the magnetic sleeve loading module 1112, and the magnetic rod loading module 1111 and the magnetic sleeve loading module 1112 are coaxially arranged. The first mechanical arm 12 comprises a magnetic sleeve mechanical arm 121 and a magnetic rod mechanical arm 122, the magnetic sleeve mechanical arm 121 can drive the magnetic sleeve loading module 1112 to move, the magnetic rod mechanical arm 122 can drive the magnetic rod loading module 1111 to move, when a sample in the deep hole plate 15 is extracted, the magnetic sleeve loading module 1112 and the magnetic rod loading module 1111 are moved into the deep hole plate 15 under the combined action of the magnetic rod mechanical arm 122 and the magnetic sleeve mechanical arm 121, and virus splitting, nucleic acid releasing, nucleic acid combining, nucleic acid purifying and the like are realized through operations such as vibration mixing, magnetic bead transferring, cleaning, eluting and the like, so that nucleic acid extraction is completed.
In order to keep consistency among a plurality of extraction areas, the first mechanical arm 12 at the plurality of extraction areas is provided with the reference plate 20, the reference plate 20 is positioned above the first mechanical arm 12 and is connected with the first mechanical arm 12, the reference plate 20 is arranged along the arrangement direction of the three magnetic rod magnetic sleeve assemblies 111, and when the nucleic acid is extracted from the samples in the deep hole plate 15, the first mechanical arm 12 in the three extraction areas synchronously moves, so that the nucleic acid of the extracted samples in the three extraction areas is kept consistent, the positioning precision is ensured, and the parallel processing of multiple samples is realized.
Specifically, as shown in fig. 6 and 7, the transfer window 2 includes a hollow transfer window box 21 horizontally arranged, an ultraviolet sterilizing lamp 8 is arranged on the inner side wall of the transfer window box 21, the transfer window 2 is located between the extraction mechanism 1 and the configuration mechanism 3, sealing windows 22 are all arranged on the top side surfaces of two ends of the transfer window box 21, movable sealing doors 23 are arranged at the sealing windows 22, the sealing windows 22 are sealed by the sealing doors 23, a deep hole plate bracket 24 is arranged in the transfer window box 21, and a fifth mechanical arm 25 for driving the deep hole plate bracket 24 to move towards the length direction of the transfer window 2 is arranged at the bottom side of the deep hole plate bracket 24. After the nucleic acid is extracted by the extraction mechanism 1, the deep-hole plate 15 is moved onto the deep-hole plate bracket 24 in the transfer window box 21 under the action of the second mechanical arm 13, and the deep-hole plate 15 is moved from one end to the other end of the transfer window box 21 under the action of the fifth mechanical arm 25, so that the deep-hole plate 15 with the extracted nucleic acid is moved to the arrangement mechanism 3 for the next operation.
Specifically, as shown in fig. 8, the configuration mechanism 3 further includes a second consumable supply drawer 34, three hole plates 35 are disposed on the second consumable supply drawer 34, and the configuration tool 33 includes four low-temperature modules 331, a sealing film module 332, an oscillation module 333, and a centrifugal module 334, where the low-temperature modules 331 may be configured as a temperature regulator, the sealing film module 332 may be configured as a sealing film machine, the oscillation module 333 may be configured as an oscillation machine, and the centrifugal module 334 may be configured as a centrifuge. When the deep hole plate 15 is moved to the position of the configuration mechanism 3 by the transfer window 2, the third mechanical arm 31 is set as a pipetting mechanical arm under the action of the third mechanical arm 31, and samples in the 12 deep hole plates 15 are transferred into the three hole plates 35. At this time, the fourth mechanical arm 32 starts to work, and the fourth mechanical arm 32 is a plate grabbing mechanical arm, so as to grab three hole plates 35, sequentially transfer the three hole plates 35 to the low temperature module 331, the film sealing module 332, the oscillation module 333 and the centrifugal module 334, sequentially perform automatic film sealing, oscillation and centrifugation, and finally transfer the configured hole plates 35 to the detection mechanism 4 for detection.
As shown in fig. 8 and 9, the detection mechanism 4 includes a large light-shielding box 41 that is hollow, an opening is provided on a side wall of the large light-shielding box 41, a detector 42 for detecting a sample is provided in the large light-shielding box 41, the configured orifice plate 35 is transferred into the large light-shielding box 41 through the opening of the large light-shielding box 41 under the action of the fourth mechanical arm 32, and the temperature control, the fluorescence imaging and the result analysis are performed on the sample in the orifice plate 35 under the action of the detector 42 in the large light-shielding box 41, so as to derive a detection result.
As shown in fig. 2 and 5, before extracting nucleic acid in a sample, the sample needs to be preprocessed, four sample loading modules 6 are arranged at the deep hole plate 15, the sample loading modules 6 are 96 sample tube arrays, a code scanning input module 5 is arranged at the extraction area, the code scanning input module 5 can be arranged as a code scanning machine, information input is carried out on the sample loading modules 6 through the code scanning input module 5 before extracting nucleic acid from the sample, the four input sample loading modules 6 move to the deep hole plate 15 of the fifth row and collide with the deep hole plate 15, the second mechanical arm 13 punctures the sample loading modules 6 by positive pressure, and the sample in the sample loading modules 6 is released into the deep hole plate 15. In order to carry out centralized processing to the released sample loading module 6, the extraction area is also provided with a garbage station 7, the garbage station 7 comprises a garbage station support 72 and a garbage station channel 71 which is arranged in a hollow mode, the top end of the garbage station channel 71 is arranged in an open mode, the inner side wall of the garbage station channel 71 is provided with an ultraviolet sterilizing lamp 8, the used sample loading module 6 is driven by a second mechanical arm 13 to discard the sample loading module 6 into the garbage station channel 71, centralized collection processing is carried out on the sample loading module 6, and meanwhile the ultraviolet sterilizing lamp 8 works, so that disinfection is carried out on the sample loading module 6.
As shown in fig. 10, the ultra-high throughput full-automatic pathogen nucleic acid detection method for the ultra-high throughput full-automatic pathogen nucleic acid detection system comprises the following steps:
s1, driving an extraction tool 11 of each extraction region in a plurality of side-by-side extraction regions to perform an operation of extracting nucleic acid from a sample by a first mechanical arm 12;
s2, transferring the sample extracted from the nucleic acid to the transfer window 2 by using the second mechanical arm 13;
s3, transferring the sample from the transfer window 2 to the configuration tool 33 by using a third mechanical arm 31;
s4, configuring the configuration tool 33 by using a fourth mechanical arm 32, and then controlling the configuration tool 33 to automatically perform operations such as film sealing, vibration, centrifugation and the like;
s5, transferring the sample processed by the configuration mechanism 3 to the detection mechanism 4 by using the fourth mechanical arm 32;
s6, the detection mechanism 4 performs temperature control, fluorescence imaging and result analysis on the processed sample to derive a detection result.
Before the step S1, the method further includes: the sample is placed into the sample loading module 6, the sample loading module 6 is subjected to code scanning information input through the code scanning input module, the sample loading module 6 with the input information is placed at the deep hole plate 15, the sample loading module 6 is positive pressure through the second mechanical arm 13, and the sample is released to enter the deep hole plate 15. After the released sample is subjected to nucleic acid extraction, virus splitting, nucleic acid release, nucleic acid combination, nucleic acid purification and the like are realized by carrying out operations such as vibration mixing, magnetic bead transfer, cleaning and elution on the sample in the deep hole plate 15, the deep hole plate 15 with the extracted nucleic acid is grabbed by the second mechanical arm 13 again, the deep hole plate 15 is moved to the transmission window 2, the deep hole plate 15 is moved to the configuration mechanism 3 through the transmission window 2, the sample in the deep hole plate 15 is moved to the hole plate 35 through the third mechanical arm 31, the hole plate 35 is sequentially moved to the low temperature module 331, the sealing membrane module 332, the vibration module and the centrifugal module 334 under the action of the fourth mechanical arm 32, and finally the sample is transferred to the detection mechanism 4 for carrying out nucleic acid detection.
It is apparent that the above examples are given by way of illustration only and are not limiting of the embodiments. Other variations or modifications of the above teachings will be apparent to those of ordinary skill in the art. It is not necessary here nor is it exhaustive of all embodiments. While still being apparent from variations or modifications that may be made by those skilled in the art are within the scope of the application.
Claims (7)
1. A super-high flux full-automatic pathogen nucleic acid detection system is characterized by comprising an extraction mechanism (1), a transmission window (2), a configuration mechanism (3) and a detection mechanism (4),
an extraction mechanism (1) comprising a plurality of extraction areas side by side, each of said extraction areas being provided with an extraction means (11) for performing high-throughput nucleic acid extraction, respectively; a first mechanical arm (12), wherein each extracting tool (11) automatically performs the operation of extracting nucleic acid from a sample under the drive of the first mechanical arm (12); a second mechanical arm (13) for transferring the sample extracted from the nucleic acid to the transfer window (2);
the configuration mechanism (3) is connected with the extraction mechanism (1) through the transmission window (2), the configuration mechanism (3) comprises a third mechanical arm (31), a fourth mechanical arm (32) and a configuration tool (33), the third mechanical arm (31) is used for transferring samples from the transmission window (2) to the configuration tool (33), the fourth mechanical arm (32) is used for configuring the configuration tool (33) and transferring samples among the configuration tools (33), and the configuration tool (33) is used for automatically performing film sealing, vibration and centrifugation;
a detection mechanism (4) for detecting nucleic acid of the sample processed by the configuration mechanism (3), and the fourth mechanical arm (32) is further used for transferring the sample processed by the configuration mechanism (3) into the detection mechanism (4);
the extraction region includes:
the device comprises a first consumable replenishment drawer (14), wherein a plurality of rows of deep hole plates (15) are arranged on the first consumable replenishment drawer (14) along the length direction of the first consumable replenishment drawer, each row of deep hole plates (15) are provided with a plurality of deep hole plates, the extraction tool (11) comprises a magnetic rod magnetic sleeve assembly (111) arranged on a first mechanical arm (12), the magnetic rod magnetic sleeve assembly (111) is driven by the first mechanical arm (12) to conduct nucleic acid extraction operation, a reference plate (20) is arranged on the first mechanical arm (12) in a plurality of extraction areas, the reference plate (20) is located above the first mechanical arm (12) and connected with the first mechanical arm (12), and the reference plate (20) is arranged along the arrangement direction of the three magnetic rod magnetic sleeve assemblies (111).
2. The ultra-high flux fully automatic pathogen nucleic acid detection system of claim 1, wherein the magnetic rod and magnetic sleeve assembly (111) comprises a magnetic rod loading module (1111) and a magnetic sleeve loading module (1112), the magnetic rod loading module (1111) and the magnetic sleeve loading module (1112) are coaxially arranged, and the first mechanical arm (12) drives the magnetic rod loading module (1111) and the magnetic rod loading module (1111) to move.
3. The ultra-high throughput fully automatic pathogen nucleic acid detection system of claim 1, wherein the configuration mechanism (3) further comprises a second consumable replenishment drawer (34), the configuration tool (33) comprises a plurality of low temperature modules (331), a sealing membrane module (332), an oscillation module (333) and a centrifugation module (334), wherein a plurality of pore plates (35) are arranged on the second consumable replenishment drawer (34), and the third mechanical arm (31) is used for transferring samples in a deep pore plate (15) positioned in the transfer window (2) into the pore plates (35).
4. The ultra-high throughput fully automatic pathogen nucleic acid detection system of claim 1, wherein the detection mechanism (4) comprises a large light shielding box (41) arranged in a hollow mode, an opening is formed in the side wall of the large light shielding box (41), and a detector (42) for detecting a sample is arranged in the large light shielding box (41).
5. The ultra-high throughput full-automatic pathogen nucleic acid detection system according to claim 1, wherein the transfer window (2) comprises a hollow transfer window box body (21), sealing windows (22) are formed in the top side surfaces of two ends of the transfer window box body (21), movable sealing doors (23) are arranged at the sealing windows (22), the sealing doors (23) seal the sealing windows (22), deep hole plate brackets (24) are arranged in the transfer window box body (21), and fifth mechanical arms (25) for driving the deep hole plate brackets (24) to move towards the length direction of the transfer window (2) are arranged at the bottom side of the deep hole plate brackets (24).
6. The ultra-high throughput fully automated pathogen nucleic acid detection system of claim 1, further comprising: the code scanning input module (5) is used for collecting information of the sample through the code scanning input module (5) before extracting nucleic acid from the sample.
7. The ultra-high throughput fully automatic pathogen nucleic acid detection system of claim 1, wherein a sample loading module (6) is further arranged at the deep-hole plate (15), a sample is located in the sample loading module (6), and the sample in the sample loading module (6) is transferred into the deep-hole plate (15) through the second mechanical arm (13).
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| CN112501006A (en) * | 2020-12-30 | 2021-03-16 | 倍仪昇智能科技(苏州)有限公司 | Automatic virus processing method |
| CN113173399B (en) * | 2021-03-15 | 2022-07-29 | 北京戴纳实验科技有限公司 | Transport case nucleic acid detecting system |
| CN113125188A (en) * | 2021-04-20 | 2021-07-16 | 深圳零一生命科技有限责任公司 | Full-automatic nucleic acid extraction instrument for fecal samples |
| CN113198360A (en) * | 2021-04-30 | 2021-08-03 | 重庆微浪自动化设备有限公司 | Vibrating magnetic equipment and vibrating magnetic attraction method for biological sample |
| CN113684120A (en) * | 2021-08-20 | 2021-11-23 | 圣湘生物科技股份有限公司 | Nucleic acid detection apparatus and nucleic acid detection method |
| CN114317230B (en) * | 2021-12-31 | 2024-04-16 | 圣湘生物科技股份有限公司 | Nucleic acid extraction and detection device and nucleic acid extraction and detection method |
| WO2023041042A1 (en) * | 2021-09-17 | 2023-03-23 | 圣湘生物科技股份有限公司 | Nucleic acid extraction and detection device and nucleic acid extraction and detection method |
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