CN112063519A - Photosynthetic bacteria continuous culture device and control method thereof - Google Patents
Photosynthetic bacteria continuous culture device and control method thereof Download PDFInfo
- Publication number
- CN112063519A CN112063519A CN202010961559.6A CN202010961559A CN112063519A CN 112063519 A CN112063519 A CN 112063519A CN 202010961559 A CN202010961559 A CN 202010961559A CN 112063519 A CN112063519 A CN 112063519A
- Authority
- CN
- China
- Prior art keywords
- culture tank
- photosynthetic bacteria
- culture
- liquid level
- pump
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/02—Photobioreactors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/22—Transparent or translucent parts
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/18—External loop; Means for reintroduction of fermented biomass or liquid percolate
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/26—Conditioning fluids entering or exiting the reaction vessel
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M31/00—Means for providing, directing, scattering or concentrating light
- C12M31/10—Means for providing, directing, scattering or concentrating light by light emitting elements located inside the reactor, e.g. LED or OLED
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M39/00—Means for cleaning the apparatus or avoiding unwanted deposits of microorganisms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
- C12M41/24—Heat exchange systems, e.g. heat jackets or outer envelopes inside the vessel
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/44—Means for regulation, monitoring, measurement or control, e.g. flow regulation of volume or liquid level
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/48—Automatic or computerized control
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Sustainable Development (AREA)
- Analytical Chemistry (AREA)
- Clinical Laboratory Science (AREA)
- Computer Hardware Design (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Thermal Sciences (AREA)
- Medicinal Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention belongs to the field of biotechnology and environmental protection, and relates to a photosynthetic bacteria continuous culture device and a control method thereof.A discharge pipe is arranged at the bottom of a culture tank, a feed pipe and a return pipe are respectively and fixedly arranged at two sides of the discharge pipe, and a feed inlet and an exhaust pipe are arranged at the top of the culture tank; a transparent sleeve with a rotating motor is rotatably arranged in the middle of the culture tank, a radiating pipe which is fixed on the culture tank and is spirally sleeved with an LED lamp strip is arranged in the transparent sleeve, a plurality of brush rods which are distributed along the circumference of the transparent sleeve are fixedly arranged on the outer side of the transparent sleeve, and a plurality of brushes are fixedly arranged on each brush rod; the utility model discloses a photosynthetic bacteria culture technique, including culture tank, slide card, light source or sensitive surface, the slide card is equipped with a plurality of level detection meter on the culture tank outer wall, and the light shadow that exists in the photobioreactor in the solution current photosynthetic bacteria culture technique leads to photosynthetic bacteria to adhere to on the light source or the sensitive surface of reactor and forms the light biomembrane, restricts the continuous automatic cultivation of photosynthetic bacteria, increases photosynthetic bacteria manufacturing cost's problem.
Description
Technical Field
The invention belongs to the field of biotechnology and environmental protection, relates to a photosynthetic bacteria continuous culture device and a control method thereof, and particularly relates to a photosynthetic bacteria continuous culture device for wastewater biological enhancement and a control method thereof.
Background
Photosynthetic bacteria are a group diversified in aspects of morphology, physiology, phylogeny and the like, and widely exist in water, soil and activated sludge in nature. In different environments, photosynthetic bacteria show various metabolic modes, and can grow by using micromolecular organic matters, sulfides and the like as electron donors under the conditions of anaerobic illumination, aerobic illumination or aerobic darkness, so that the water quality is purified. The photosynthetic bacteria have various functions of nitrogen fixation, denitrification, carbon fixation, sulfide oxidation and the like, are closely related to the circulation of nitrogen, phosphorus and sulfur in the water body, and play an important role in the self-purification process of the water body. Therefore, the photosynthetic bacteria have been widely used in aquaculture, biologically enhanced treatment and resource utilization of black and odorous water and organic wastewater.
At present, the photosynthetic bacteria are cultured mainly in an artificial culture mode, and automatic continuous culture is difficult to realize. The main reason for this is the problem of "light shading" in photobioreactors: because the photosynthetic bacteria have phototaxis, the photosynthetic bacteria are easily concentrated and attached to the light source or the light receiving surface of the reactor to form a compact photobiological film, and further transmission of light is blocked. This makes people have to wash the light source or the sensitive surface of the reactor frequently, limits the continuous automatic culture of the photosynthetic bacteria, and increases the production cost of the photosynthetic bacteria.
Disclosure of Invention
In view of the above, the present invention provides a continuous culture apparatus for photosynthetic bacteria, which has a simple structure and a low production cost and can be used for large-scale culture, and a control method thereof, in order to solve the problems that photosynthetic bacteria are attached to a light source or a light receiving surface of a reactor to form a light biofilm due to light shadows existing in a photobioreactor in the existing photosynthetic bacteria culture technology, so as to limit continuous automatic culture of the photosynthetic bacteria, and increase production cost of the photosynthetic bacteria.
In order to achieve the aim, the invention provides a continuous culture device for photosynthetic bacteria, which comprises a culture tank and a storage tank arranged outside the culture tank, wherein the bottom of the culture tank is provided with a discharge pipe, two sides of the discharge pipe are respectively and fixedly provided with a feed pipe communicated with the storage tank and a return pipe convenient for the circulation of bacterial liquid in the culture tank, the feed pipe is connected with two branch pipes, one branch is connected with the storage tank through a feed pump, the other branch is connected with tap water through a feed pump, the drain pipe is provided with a drainage pump, the return pipe is connected with a circulating pump, and the top of the culture tank is provided with a feed port and;
a transparent sleeve with a rotating motor is rotatably arranged in the middle of the culture tank, a radiating pipe which is fixed on the culture tank and is provided with an LED lamp strip in a spiral sleeve is arranged in the transparent sleeve, a liquid seal box is fixedly arranged at the joint of the transparent sleeve and the culture tank, a plurality of brush rods which are circumferentially distributed along the transparent sleeve are fixedly arranged on the outer side of the transparent sleeve, the brush rods are fixedly connected with the culture tank, a plurality of brushes which are uniformly distributed along the radial direction of the transparent sleeve are fixedly arranged on each brush rod, and the free ends of the brushes are abutted against the outer wall of the transparent sleeve;
fixed mounting has the lateral wall scale on the cultivation jar outer wall, and sliding connection has a plurality of level detection meter on the lateral wall scale, and level detection meter, charge pump, intake pump, circulating pump and drain pump are connected on same programmable controller.
The beneficial effect of this basic scheme lies in: the liquid seal box plays the effect of sealed transparent sleeve pipe and culture tank junction, prevents that the culture solution in the culture tank from getting into transparent sleeve pipe and damaging cooling tube and LED lamp area. The rotating electrical machines drives the transparent sleeve to rotate so that the outer wall of the transparent sleeve continuously rotates along the brush rod, and photosynthetic bacteria remained on the outer wall of the transparent sleeve are scraped to prevent the photosynthetic bacteria from being gathered on the outer wall of the transparent sleeve. The circulating pump continuously circulates the bacterial liquid at the conical bottom of the culture tank. According to the detection data of different liquid level detection meters, the opening and closing of the feeding pump, the water inlet pump, the circulating pump and the drainage pump can be controlled in real time through the programmable controller.
Further, inlay in the culture tank and be equipped with the support frame that is used for supporting cooling tube and transparent sleeve, the upper cover plate is installed at the top of culture tank, and the sealed laminating of upper cover plate and cylinder culture tank inner wall, liquid seal box install on the upper cover plate, charge door and blast pipe are installed on the upper cover plate, and the circular spacing hole with transparent sleeve looks adaptation is seted up at the support frame middle part. Has the advantages that: the upper end of the transparent sleeve is fixed on a bearing of the rotating motor, the lower end of the transparent sleeve penetrates through the support frame and can rotate in the range of the limiting hole, and the transparent sleeve is driven by the rotating motor to rotate.
Furthermore, the lateral wall scale is provided with a plurality of U-shaped clamping grooves in a clamped mode, each U-shaped clamping groove is provided with a positioning bolt in a threaded connection mode, each U-shaped clamping groove is provided with a liquid level detection meter with a data line and an infrared emission window, the liquid level detection meters slide on the lateral wall scale through the corresponding U-shaped clamping grooves, and the infrared emission windows of the liquid level detection meters are tightly attached to the outer wall of the culture tank. Has the advantages that: the cooperation of level gauge and lateral wall scale is convenient for the accurate measurement of programmable controller fungus liquid's volume in the culture tank.
Furthermore, the upper end of the brush rod is fixed on the upper cover plate of the culture tank, and the lower end is fixed on the support frame.
Furthermore, a plurality of round holes are arranged on the part of the transparent sleeve extending out of the liquid seal box and exposed outside the culture tank. Has the advantages that: the round hole on the transparent sleeve is beneficial to discharging hot gas in the transparent sleeve.
Furthermore, the number of the liquid level detection meters is 4, the warning liquid level, the reaction liquid level, the feed supplement liquid level and the liquid level are sequentially indicated from top to bottom, the ratio of the volume corresponding to the liquid level to the effective volume of the culture tank is 1: 20-1: 3, and the ratio of the volume corresponding to the feed supplement liquid level to the effective volume of the culture tank is 1: 10-1: 2.
Furthermore, an overflow pipe is arranged on the outer wall of the culture tank between the reaction liquid level and the warning liquid level. Has the advantages that: is convenient for discharging the redundant bacteria liquid in the culture tank to an external system.
Further, the bottom of the culture tank is a conical bottom. Has the advantages that: the culture tank with the conical bottom is convenient for discharging bacterial liquid.
Further, the thickness of the single brush rod is not more than 0.5 cm.
Further, the radiating pipe is made of aluminum-based or copper-based material and the interior of the radiating pipe is hollow. Has the advantages that: the hollow radiating pipe is convenient for radiating heat in the radiating pipe.
Furthermore, the culture tank and the transparent sleeve are made of organic glass or toughened glass. Has the advantages that: the culture tank and the transparent sleeve made of organic glass or toughened glass are convenient for observing the culture condition of the bacteria liquid in real time.
A chemical control method of a photosynthetic bacteria continuous culture device comprises the following steps:
A. after the photosynthetic bacteria continuous culture device is assembled, microorganism liquid is manually added to the liquid level from a feed inlet at the top of the culture tank;
B. the intelligent control system is started, the feeding pump is started under the control of the programmable controller, the culture medium is pumped from the storage tank to the culture tank, when the liquid supplement level is reached, the feeding pump is automatically closed, the water inlet pump is automatically started, when the reaction level is reached, the water inlet pump is automatically closed, the circulating pump is automatically started, after the circulating pump is started, the LED lamp strip and the rotating motor in the lighting system are automatically started, and the microorganisms in the culture tank start to be cultured;
C. after the microorganisms are cultured for a set time, the LED lamp strip and the circulating pump in the lighting system are automatically closed, the drainage pump on the discharge pipe is opened, the cultured bacterial liquid is put into an external system, and when the liquid level of the bacterial liquid drops to the liquid level of the seed liquid, the drainage pump on the discharge pipe is automatically closed;
D. and C, starting a feeding pump, repeating the step B and the step C, and culturing the photosynthetic bacteria in the next period, so as to realize intelligent control of continuous culture and continuous feeding of the photosynthetic bacteria.
The invention has the beneficial effects that:
1. according to the continuous photosynthetic bacteria culture device disclosed by the invention, the transparent sleeve is connected to the rotating motor, the rotating motor drives the transparent sleeve to rotate, so that the brush rod is continuously scraped along photosynthetic bacteria on the inner wall of the culture tank, a biological film on the surface of a light source is removed in real time, the light attenuation caused by the formation of the biological film in the photobioreactor is avoided, the cost of manual removal is saved, and the photobioreactor can continuously and automatically run.
2. The photosynthetic bacteria continuous culture device disclosed by the invention realizes the removal of the biofilm on the surface of the light source by adopting the mode that the central transparent sleeve rotates instead of the outer side brush rotates, and is favorable for ensuring the sealing effect between the rotating motor and the culture tank. The lighting system has a self-cleaning function, can remove a biological film on the surface of a light source at any time, and realizes the continuous culture and release of photosynthetic bacteria by combining a time control system and a liquid level control system. The device has the advantages of simple and firm structure, simple and convenient operation management and labor maintenance cost saving.
3. The photosynthetic bacteria continuous culture device disclosed by the invention intelligently controls the operation of the lighting system and the water inlet and outlet system through the culture time and the liquid level detector, thereby realizing the continuous culture and addition of photosynthetic bacteria, greatly reducing the difficulty of operation management and improving the effect of biological enhancement.
Additional advantages, objects, and features of the invention will be set forth in part in the description which follows and in part will become apparent to those having ordinary skill in the art upon examination of the following or may be learned from practice of the invention. The objectives and other advantages of the invention may be realized and attained by the means of the instrumentalities and combinations particularly pointed out hereinafter.
Drawings
For the purposes of promoting a better understanding of the objects, aspects and advantages of the invention, reference will now be made to the following detailed description taken in conjunction with the accompanying drawings in which:
FIG. 1 is a schematic structural diagram of a continuous culture apparatus for photosynthetic bacteria according to the present invention;
FIG. 2 is an assembly view of a lamp strip in the continuous culture apparatus for photosynthetic bacteria according to the present invention;
FIG. 3 is a schematic structural view of a liquid level detector in the continuous culture apparatus for photosynthetic bacteria according to the present invention;
FIG. 4 is a diagram showing the actual culture effect of Rhodopseudomonas palustris cultured by the continuous culture apparatus for photosynthetic bacteria of the present invention.
Reference numerals: the device comprises a storage tank 1, a feeding pump 2, a water inlet pump 3, a draining pump 4, a circulating pump 5, a discharging pipe 6, a feeding pipe 7, a culture tank 8, a conical bottom 9, a support frame 10, an upper cover plate 11, a feeding port 12, a rotating motor 13, a liquid seal box 14, a radiating pipe 15, a brush rod 16, a return pipe 17, an overflow pipe 18, an exhaust pipe 19, a side wall scale 20, a liquid level detector 21, a liquid level 22, a feed supplement liquid level 23, a reaction liquid level 24, a warning liquid level 25, a programmable controller 26, a brush 27, an LED lamp strip 28, a transparent sleeve 29, an infrared emission window 30, a data line 31, a U-shaped clamping groove 32 and a positioning bolt 33.
Detailed Description
The embodiments of the present invention are described below with reference to specific embodiments, and other advantages and effects of the present invention will be easily understood by those skilled in the art from the disclosure of the present specification. The invention is capable of other and different embodiments and of being practiced or of being carried out in various ways, and its several details are capable of modification in various respects, all without departing from the spirit and scope of the present invention. It should be noted that the drawings provided in the following embodiments are only for illustrating the basic idea of the present invention in a schematic way, and the features in the following embodiments and examples may be combined with each other without conflict.
Wherein the showings are for the purpose of illustrating the invention only and not for the purpose of limiting the same, and in which there is shown by way of illustration only and not in the drawings in which there is no intention to limit the invention thereto; to better illustrate the embodiments of the present invention, some parts of the drawings may be omitted, enlarged or reduced, and do not represent the size of an actual product; it will be understood by those skilled in the art that certain well-known structures in the drawings and descriptions thereof may be omitted.
The same or similar reference numerals in the drawings of the embodiments of the present invention correspond to the same or similar components; in the description of the present invention, it should be understood that if there is an orientation or positional relationship indicated by terms such as "upper", "lower", "left", "right", "front", "rear", etc., based on the orientation or positional relationship shown in the drawings, it is only for convenience of description and simplification of description, but it is not an indication or suggestion that the referred device or element must have a specific orientation, be constructed in a specific orientation, and be operated, and therefore, the terms describing the positional relationship in the drawings are only used for illustrative purposes, and are not to be construed as limiting the present invention, and the specific meaning of the terms may be understood by those skilled in the art according to specific situations.
The continuous photosynthetic bacteria culture device shown in figures 1-3 comprises a culture tank 8 and a material storage tank 1 arranged outside the culture tank 8, wherein the culture tank 8 is made of transparent materials such as organic glass or toughened glass. The bottom of culture tank 8 is conical bottom 9, and 9 mid-mounting at the conical bottom of culture tank 8 have row material pipe 6, arrange and expect that there are inlet pipe 7 and back flow 17 on the conical bottom 9 of 6 both sides respectively fixed mounting, are connected with two branch road pipes on the inlet pipe 7, and one of them branch road passes through charge pump 2 and connects storage tank 1, and another branch road passes through intake pump 3 and connects the running water. The drain pipe is provided with a drain pump 4, the return pipe 17 is connected with a circulating pump 5, and the circulating pump 5 continuously circulates the bacterial liquid at the conical bottom 9 of the culture tank.
The middle part of the culture tank 8 is rotatably provided with a transparent sleeve with a rotating motor 13, and the rotating speed of the rotating motor 13 is not more than 60 r/min. Transparent sleeve 29 comprises transparent materials such as organic glass or toughened glass, places the cooling tube 15 of fixing on culture tank 8 in the transparent sleeve, and cooling tube 15 can maintain the interior fungus liquid temperature of culture tank 8. The heat dissipating pipe 15 is made of an aluminum-based or copper-based material, and has a hollow interior. The LED lamp strip 28 is spirally sleeved on the radiating pipe 15, and the illumination intensity of the LED lamp strip 28 is in the range of 1500-5000 lux. LED lamp area 28 spiral winding and adhesion are on cooling tube 15, and transparent sleeve 29 cover is outside taking LED lamp area 28's cooling tube 15, and transparent sleeve 29 and 8 junction fixed mounting of culture tank have a liquid seal box 14, and liquid seal box 14 plays the effect of sealed transparent sleeve 29 and 8 junctions of culture tank, prevents that the culture solution in the culture tank 8 from getting into transparent sleeve 29 and damaging cooling tube 15 and LED lamp area 28. The radiating pipe 15 and the LED strip 28 located in the transparent sleeve 29 are not connected to the rotating motor 13 and do not rotate with the rotating motor 13. Transparent sleeve 29 outside fixed mounting has 2 ~ 4 brush-holder stud 16 along transparent sleeve 29 circumference distribution, brush- holder stud 16 and 8 fixed connection of culture tank, fixed mounting has a plurality of root brushes 27 along the radial evenly distributed of transparent sleeve 29 on every brush-holder stud 16, brush 27 free end offsets with transparent sleeve 29 outer wall, rotating electrical machines 13 drives transparent sleeve and rotates and make transparent sleeve outer wall constantly rotate along brush-holder stud 16, to the photosynthetic bacterium scraping of remaining on the transparent sleeve outer wall, prevent photosynthetic bacterium gathering on transparent sleeve outer wall. In this embodiment, two brush rods 16 are taken as an example, the two brush rods 16 are aligned with the central axis of the transparent sleeve 29, and the thickness of a single brush rod 16 is not more than 0.5 cm.
The culture tank 8 on the upper part of the conical bottom 9 is embedded with a support frame 10 for supporting a radiating pipe 15 and a transparent sleeve, the top of the culture tank 8 is provided with an upper cover plate 11, the upper cover plate 11 is in sealing fit with the inner wall of the cylindrical culture tank 8, and a liquid seal box 14 is arranged on the upper cover plate 11. The upper cover plate 11 is also provided with a charging opening 12 and an exhaust pipe 19. The middle part of the support frame 10 is provided with a round limiting hole matched with the transparent sleeve 29, the upper end of the transparent sleeve 29 is fixed on a bearing of the rotating motor 13, the lower end of the transparent sleeve 29 penetrates through the support frame 10 and can rotate in the limiting hole range, and the transparent sleeve 29 is driven by the rotating motor 13 to rotate. 1-5 round holes are formed in the part, exposed outside the culture tank 8, of the transparent sleeve 29 extending out of the liquid seal box 14, and hot air in the transparent sleeve 29 is discharged conveniently. The upper end of the brush rod 16 is fixed on the upper cover plate 11 of the culture tank 8, and the lower end is fixed on the support frame 10.
Fixed mounting has lateral wall scale 20 on 8 outer walls of culture tank, the slip card is equipped with 3 ~ 8U type draw-in grooves 32 on the lateral wall scale 20, equal threaded connection has positioning bolt 33 on every U type draw-in groove 32, all install the level detection meter 21 of taking data line 31 and infrared emission window 30 on every U type draw-in groove, the U type draw-in groove that the accessible of level detection meter 21 corresponds slides on lateral wall scale 20, every level detection meter 21 all is fixed in on lateral wall scale 20 through positioning bolt 33 on the corresponding U type draw-in groove, infrared emission window 30 of level detection meter 21 hugs closely on the cylinder lateral wall. The liquid level detection meter 21, the feeding pump 2, the water inlet pump 3, the circulating pump 5 and the draining pump 4 are connected to the same programmable controller 26. In this embodiment, 4 level detectors 21 are used as an example to identify the guard level 25, the reaction level 24, the feed level 23 and the seed level 22 of the culture tank from top to bottom. Wherein the ratio of the volume corresponding to the liquid level 22 to the effective volume of the culture tank 8 is 1:20 to 1:3, and the ratio of the volume corresponding to the feed liquid level 23 to the effective volume of the culture tank 8 is 1:10 to 1: 2. An overflow pipe 18 is arranged on the outer wall of the culture tank 8 between the reaction liquid level 24 and the warning liquid level 25, so that the redundant bacteria liquid in the culture tank 8 can be conveniently discharged to an external system.
The intelligent control method of the photosynthetic bacterium continuous culture device specifically comprises the following steps:
A. after the photosynthetic bacteria continuous culture device is assembled, microorganism liquid is manually added from a feed inlet 12 at the top of the culture tank 8 to a liquid level 22;
B. the intelligent control system is started, the feed pump 2 is started under the control of the programmable controller 26, the culture medium is pumped from the storage tank 1 to the culture tank 8, when the feed supplement liquid level 23 is reached, the feed pump 2 is automatically closed, the water inlet pump 3 is automatically started, when the reaction liquid level 24 is reached, the water inlet pump 3 is automatically closed, the circulating pump 5 is automatically started, after the circulating pump 5 is started, the LED lamp strip 28 and the rotating motor 13 in the lighting system are automatically started, and the microorganisms in the culture tank 8 start to be cultured;
C. after the microorganisms are cultured for a set time, the LED lamp strip 28 and the circulating pump 5 in the illuminating system are automatically closed, the drainage pump 4 on the discharge pipe 6 is opened, the cultured bacterial liquid is put into an external system, and when the liquid level of the bacterial liquid drops to the liquid level 22, the drainage pump 4 on the discharge pipe 6 is automatically closed;
D. and C, starting the feeding pump 2, repeating the step B and the step C, and culturing the photosynthetic bacteria in the next period, so that the intelligent control of continuous culture and continuous feeding of the photosynthetic bacteria is realized, wherein in the continuous culture process of the photosynthetic bacteria, the upper part of the culture tank 8 is provided with a warning liquid level 25, when the culture tank 8 breaks down to enable the feed liquid in the culture tank to exceed the warning liquid level 25, the drainage pump 4 is automatically started to drain water, and the drainage pump 4 is closed until the liquid level is reduced to a reaction liquid level 24.
Examples
Taking the cultivation of rhodopseudomonas palustris as an example, the effective volume of the cultivation tank 8 is 100L, the radiating pipes 15 are aluminum-based radiating pipes 15, and the LED lamp strip 28 is a 5055-type LED warm white light bar. The transparent sleeve 29 and the main culture tank 8 are sealed by a liquid seal box 14, and tap water is filled in the liquid seal box 14. Three hair brushes 27 are arranged outside the transparent sleeve 29.
The culture control method of the rhodopseudomonas palustris continuous culture device specifically comprises the following steps:
A. after the continuous culture device for the rhodopseudomonas palustris is assembled, setting the volume corresponding to the liquid level 22 of a culture tank 8 to be 10L, the volume corresponding to a feed liquid level 23 to be 20L, the volume corresponding to a reaction liquid level 24 to be 100L, the volume corresponding to a warning liquid level 25 to be 102L, and culturing for 72h, wherein the culturing period is set to be 3 periods, filling a concentrated rhodopseudomonas palustris culture medium in a storage tank 1, and manually adding the rhodopseudomonas palustris liquid to the liquid level 22(10L) from a feed opening 12 at the top of the culture tank 8;
B. the intelligent control system is started, the feed pump 2 is started under the control of the programmable controller 26, the culture medium is pumped from the storage tank 1 to the culture tank 8, when the feed supplement liquid level reaches 23(20L), the feed pump 2 is automatically closed, the water inlet pump 3 is automatically started, when the reaction liquid level reaches 24(100L), the water inlet pump 3 is automatically closed, the circulating pump 5 is automatically started, after the circulating pump 5 is started, the LED lamp strip 28 and the rotating motor 13 in the lighting system are automatically started, and the microorganisms in the culture tank 8 start to be cultured;
C. after the microorganisms are cultured for 72 hours, the LED lamp strip 28 and the circulating pump 5 in the lighting system are automatically closed, the drainage pump 4 on the discharge pipe 6 is opened, the cultured bacterial liquid is put into an external system, and when the liquid level of the bacterial liquid is reduced to the liquid level 22(10L), the drainage pump 4 on the discharge pipe 6 is automatically closed;
D. and (3) starting the feeding pump 2, repeating the step (B) and the step (C) (when the liquid level rises to a feed supplement liquid level 23, stopping the feeding pump 2, starting the water inlet pump 3, and when the liquid level rises to a reaction liquid level 24, closing the water inlet pump 3, and starting the circulating pump 5 and the lighting system) twice, so that 3 periods of continuous culture of the rhodopseudomonas palustris is realized, and the intelligent control of continuous culture and continuous feeding of the rhodopseudomonas palustris is realized. The upper part of the culture tank 8 is provided with a warning liquid level 25, when the culture tank 8 breaks down and the feed liquid in the culture tank exceeds the warning liquid level 25, the drainage pump 4 automatically starts drainage until the liquid level is reduced to the reaction liquid level 24, and the drainage pump 4 is closed.
The actual culture effect of Rhodopseudomonas palustris is shown in FIG. 4. The result shows that the invention can better realize the continuous culture and continuous feeding of the rhodopseudomonas palustris, and the bacterial liquid concentration in the culture of 3 continuous periods is respectively 35 hundred million/ml, 32 hundred million/ml and 33 hundred million/ml, thereby reaching the concentration specified by the NY 527-2002 standard.
Finally, the above embodiments are only intended to illustrate the technical solutions of the present invention and not to limit the present invention, and although the present invention has been described in detail with reference to the preferred embodiments, it will be understood by those skilled in the art that modifications or equivalent substitutions may be made on the technical solutions of the present invention without departing from the spirit and scope of the technical solutions, and all of them should be covered by the claims of the present invention.
Claims (10)
1. A photosynthetic bacteria continuous culture device is characterized by comprising a culture tank and a storage tank arranged outside the culture tank, wherein the bottom of the culture tank is provided with a discharge pipe, two sides of the discharge pipe are respectively and fixedly provided with a feed pipe communicated with the storage tank and a return pipe convenient for the circulation of bacterial liquid in the culture tank, the feed pipe is connected with two branch pipes, one branch is connected with the storage tank through a feed pump, the other branch is connected with tap water through a feed pump, the drain pipe is provided with a drain pump, the return pipe is connected with a circulating pump, and the top of the culture tank is provided with a feed inlet and an exhaust pipe;
a transparent sleeve with a rotating motor is rotatably arranged in the middle of the culture tank, a radiating pipe which is fixed on the culture tank and is provided with an LED lamp strip in a spiral sleeve is arranged in the transparent sleeve, a liquid seal box is fixedly arranged at the joint of the transparent sleeve and the culture tank, a plurality of brush rods which are circumferentially distributed along the transparent sleeve are fixedly arranged on the outer side of the transparent sleeve, the brush rods are fixedly connected with the culture tank, a plurality of brushes which are uniformly distributed along the radial direction of the transparent sleeve are fixedly arranged on each brush rod, and the free ends of the brushes are abutted against the outer wall of the transparent sleeve;
fixed mounting has the lateral wall scale on the cultivation jar outer wall, and sliding connection has a plurality of level detection meter on the lateral wall scale, and level detection meter, charge pump, intake pump, circulating pump and drain pump are connected on same programmable controller.
2. A continuous culture apparatus for photosynthetic bacteria as claimed in claim 1 wherein the culture tank is embedded with a support frame for supporting the heat dissipation pipe and the transparent sleeve, the top of the culture tank is mounted with an upper cover plate which is sealed with the inner wall of the cylindrical culture tank, the liquid seal box is mounted on the upper cover plate, the feed inlet and the exhaust pipe are mounted on the upper cover plate, and the middle of the support frame is provided with a circular limiting hole adapted to the transparent sleeve.
3. A photosynthetic bacteria continuous culture device as claimed in claim 1, wherein the side wall scale is provided with a plurality of U-shaped slots, each U-shaped slot is connected with a positioning bolt by screw thread, each U-shaped slot is provided with a liquid level detector with a data line and an infrared emission window, the liquid level detector slides on the side wall scale through the corresponding U-shaped slot, and the infrared emission window of the liquid level detector is tightly attached to the outer wall of the culture tank.
4. A continuous culture apparatus for photosynthetic bacteria as claimed in claim 2 wherein the upper end of the brush rod is fixed to the upper cover plate of the culture tank and the lower end is fixed to the support frame.
5. A continuous culture device for photosynthetic bacteria as claimed in claim 1 wherein the transparent casing has a plurality of circular holes extending from the liquid-tight box to the outside of the culture tank.
6. A photosynthetic bacteria continuous culture apparatus according to claim 1 wherein the number of the liquid level detectors is 4, and the warning liquid level, the reaction liquid level, the feed liquid level and the liquid level are indicated in this order from top to bottom, the ratio of the volume corresponding to the liquid level to the effective volume of the culture tank is 1:20 to 1:3, and the ratio of the volume corresponding to the feed liquid level to the effective volume of the culture tank is 1:10 to 1: 2.
7. A continuous culture apparatus for photosynthetic bacteria as claimed in claim 6 wherein the overflow pipe is installed on the outer wall of the culture tank between the reaction liquid level and the warning liquid level.
8. A photosynthetic bacteria continuous culture apparatus according to claim 1, wherein the bottom of the culture tank is a tapered bottom, and the heat radiating pipe is made of an aluminum-based or copper-based material and has a hollow interior.
9. A photosynthetic bacteria continuous culture device according to claim 1, characterized in that the culture tank and the transparent casing are made of organic glass or toughened glass.
10. A chemical control method of a photosynthetic bacteria continuous culture device is characterized by comprising the following steps:
A. after the photosynthetic bacteria continuous culture device is assembled, microorganism liquid is manually added to the liquid level from a feed inlet at the top of the culture tank;
B. the intelligent control system is started, the feeding pump is started under the control of the programmable controller, the culture medium is pumped from the storage tank to the culture tank, when the liquid supplement level is reached, the feeding pump is automatically closed, the water inlet pump is automatically started, when the reaction level is reached, the water inlet pump is automatically closed, the circulating pump is automatically started, after the circulating pump is started, the LED lamp strip and the rotating motor in the lighting system are automatically started, and the microorganisms in the culture tank start to be cultured;
C. after the microorganisms are cultured for a set time, the LED lamp strip and the circulating pump in the lighting system are automatically closed, the drainage pump on the discharge pipe is opened, the cultured bacterial liquid is put into an external system, and when the liquid level of the bacterial liquid drops to the liquid level of the seed liquid, the drainage pump on the discharge pipe is automatically closed;
D. and C, starting a feeding pump, repeating the step B and the step C, and culturing the photosynthetic bacteria in the next period, so as to realize intelligent control of continuous culture and continuous feeding of the photosynthetic bacteria.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010961559.6A CN112063519B (en) | 2020-09-14 | 2020-09-14 | Photosynthetic bacteria continuous culture device and control method thereof |
| PCT/CN2021/076203 WO2022052418A1 (en) | 2020-09-14 | 2021-02-09 | Continuous culture apparatus for photosynthetic bacteria and control method therefor |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010961559.6A CN112063519B (en) | 2020-09-14 | 2020-09-14 | Photosynthetic bacteria continuous culture device and control method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN112063519A true CN112063519A (en) | 2020-12-11 |
| CN112063519B CN112063519B (en) | 2022-06-10 |
Family
ID=73697117
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010961559.6A Active CN112063519B (en) | 2020-09-14 | 2020-09-14 | Photosynthetic bacteria continuous culture device and control method thereof |
Country Status (2)
| Country | Link |
|---|---|
| CN (1) | CN112063519B (en) |
| WO (1) | WO2022052418A1 (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112638098A (en) * | 2021-02-05 | 2021-04-09 | 宁波康加分电器科技有限公司 | Heat dissipation base capable of continuously working |
| WO2022052418A1 (en) * | 2020-09-14 | 2022-03-17 | 中国科学院重庆绿色智能技术研究院 | Continuous culture apparatus for photosynthetic bacteria and control method therefor |
| CN114606103A (en) * | 2022-03-16 | 2022-06-10 | 德默特生物科技(珠海)有限公司 | Hybrid photobioreactor |
Citations (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101402915A (en) * | 2008-08-05 | 2009-04-08 | 上海海洋大学 | High-efficiency photobioreactor for cultivation of photosynthetic bacteria |
| WO2010115996A1 (en) * | 2009-04-10 | 2010-10-14 | Acta Alga | Photobioreactor in a closed medium for cultivating photosynthetic micro-organisms |
| CN201915083U (en) * | 2010-09-13 | 2011-08-03 | 江苏邦德生物科技发展有限公司 | Efficient culturing device for photosynthetic bacteria |
| CN202415537U (en) * | 2012-01-18 | 2012-09-05 | 河北万林生物科技有限公司 | Photosynthetic bacteria culture apparatus |
| CN102994367A (en) * | 2012-11-22 | 2013-03-27 | 华侨大学 | High-efficiency phototroph reaction system for pure culture of photosynthetic bacteria and sterilizing method thereof |
| JP2015053872A (en) * | 2013-09-10 | 2015-03-23 | 株式会社日立製作所 | Culture tank for photosynthetic microorganism and culture apparatus for photosynthetic microorganism |
| CN204224591U (en) * | 2014-11-24 | 2015-03-25 | 新疆光合元生物科技有限公司 | Photosynthesis microorganism fermentation tank |
| CN205046109U (en) * | 2015-09-10 | 2016-02-24 | 天津现代职业技术学院 | Photosynthetic bacteria cultivation jar |
| CN106119083A (en) * | 2016-08-22 | 2016-11-16 | 浙江浙大水业有限公司 | A kind of solar energy photosynthetic bacteria scene continuous production device |
| CN106434412A (en) * | 2016-06-27 | 2017-02-22 | 郭洪伟 | Nitrobacterium continuous production method and production equipment thereof |
| CN206721190U (en) * | 2017-03-24 | 2017-12-08 | 招商局生态环保科技有限公司 | Batch-type microorganism incubator |
| CN206828210U (en) * | 2017-03-27 | 2018-01-02 | 招商局生态环保科技有限公司 | The PLC control system of batch-type complex microorganism incubation equipment |
| CN207347568U (en) * | 2017-10-19 | 2018-05-11 | 中冶华天工程技术有限公司 | The Photosynthetic bacteria culturing device of automatic light source |
| CN207596866U (en) * | 2017-10-19 | 2018-07-10 | 浙江浙大水业有限公司 | A kind of on-line equipment that bacterium solution is continuously cultivated using particulate media |
| US20180371391A1 (en) * | 2015-06-17 | 2018-12-27 | Siftex Equipment Company, Inc. | Pure algae growth system and method |
| CN209226965U (en) * | 2018-12-12 | 2019-08-09 | 河西学院 | A kind of microalgae bactogen preventing microalgae adherence |
| CN209555234U (en) * | 2019-01-09 | 2019-10-29 | 高建军 | A kind of cultivation bucket |
| CN110699252A (en) * | 2019-11-08 | 2020-01-17 | 安徽德宝生物科技有限公司 | Algae continuous culture reaction system |
| CN209974780U (en) * | 2019-03-23 | 2020-01-21 | 上海傲中生物工程设备有限公司 | Pilot scale fermentation cylinder of ration feed supplement |
| CN111004723A (en) * | 2019-12-30 | 2020-04-14 | 重庆文理学院 | Device capable of improving secondary metabolites of honeysuckle and working method |
| WO2022052418A1 (en) * | 2020-09-14 | 2022-03-17 | 中国科学院重庆绿色智能技术研究院 | Continuous culture apparatus for photosynthetic bacteria and control method therefor |
-
2020
- 2020-09-14 CN CN202010961559.6A patent/CN112063519B/en active Active
-
2021
- 2021-02-09 WO PCT/CN2021/076203 patent/WO2022052418A1/en active Application Filing
Patent Citations (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101402915A (en) * | 2008-08-05 | 2009-04-08 | 上海海洋大学 | High-efficiency photobioreactor for cultivation of photosynthetic bacteria |
| WO2010115996A1 (en) * | 2009-04-10 | 2010-10-14 | Acta Alga | Photobioreactor in a closed medium for cultivating photosynthetic micro-organisms |
| CN201915083U (en) * | 2010-09-13 | 2011-08-03 | 江苏邦德生物科技发展有限公司 | Efficient culturing device for photosynthetic bacteria |
| CN202415537U (en) * | 2012-01-18 | 2012-09-05 | 河北万林生物科技有限公司 | Photosynthetic bacteria culture apparatus |
| CN102994367A (en) * | 2012-11-22 | 2013-03-27 | 华侨大学 | High-efficiency phototroph reaction system for pure culture of photosynthetic bacteria and sterilizing method thereof |
| JP2015053872A (en) * | 2013-09-10 | 2015-03-23 | 株式会社日立製作所 | Culture tank for photosynthetic microorganism and culture apparatus for photosynthetic microorganism |
| CN204224591U (en) * | 2014-11-24 | 2015-03-25 | 新疆光合元生物科技有限公司 | Photosynthesis microorganism fermentation tank |
| US20180371391A1 (en) * | 2015-06-17 | 2018-12-27 | Siftex Equipment Company, Inc. | Pure algae growth system and method |
| CN205046109U (en) * | 2015-09-10 | 2016-02-24 | 天津现代职业技术学院 | Photosynthetic bacteria cultivation jar |
| CN106434412A (en) * | 2016-06-27 | 2017-02-22 | 郭洪伟 | Nitrobacterium continuous production method and production equipment thereof |
| CN106119083A (en) * | 2016-08-22 | 2016-11-16 | 浙江浙大水业有限公司 | A kind of solar energy photosynthetic bacteria scene continuous production device |
| CN206721190U (en) * | 2017-03-24 | 2017-12-08 | 招商局生态环保科技有限公司 | Batch-type microorganism incubator |
| CN206828210U (en) * | 2017-03-27 | 2018-01-02 | 招商局生态环保科技有限公司 | The PLC control system of batch-type complex microorganism incubation equipment |
| CN207347568U (en) * | 2017-10-19 | 2018-05-11 | 中冶华天工程技术有限公司 | The Photosynthetic bacteria culturing device of automatic light source |
| CN207596866U (en) * | 2017-10-19 | 2018-07-10 | 浙江浙大水业有限公司 | A kind of on-line equipment that bacterium solution is continuously cultivated using particulate media |
| CN209226965U (en) * | 2018-12-12 | 2019-08-09 | 河西学院 | A kind of microalgae bactogen preventing microalgae adherence |
| CN209555234U (en) * | 2019-01-09 | 2019-10-29 | 高建军 | A kind of cultivation bucket |
| CN209974780U (en) * | 2019-03-23 | 2020-01-21 | 上海傲中生物工程设备有限公司 | Pilot scale fermentation cylinder of ration feed supplement |
| CN110699252A (en) * | 2019-11-08 | 2020-01-17 | 安徽德宝生物科技有限公司 | Algae continuous culture reaction system |
| CN111004723A (en) * | 2019-12-30 | 2020-04-14 | 重庆文理学院 | Device capable of improving secondary metabolites of honeysuckle and working method |
| WO2022052418A1 (en) * | 2020-09-14 | 2022-03-17 | 中国科学院重庆绿色智能技术研究院 | Continuous culture apparatus for photosynthetic bacteria and control method therefor |
Non-Patent Citations (2)
| Title |
|---|
| 师玉忠: "光合细菌连续制氢工艺及相关机理研究", 《中国博士学位论文库 工程科技Ⅰ辑》 * |
| 杨晋晖: "光合微生物制氢菌种连续培养***及其装置研究", 《中国优秀硕士学位论文库 工程科技Ⅰ辑》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2022052418A1 (en) * | 2020-09-14 | 2022-03-17 | 中国科学院重庆绿色智能技术研究院 | Continuous culture apparatus for photosynthetic bacteria and control method therefor |
| CN112638098A (en) * | 2021-02-05 | 2021-04-09 | 宁波康加分电器科技有限公司 | Heat dissipation base capable of continuously working |
| CN114606103A (en) * | 2022-03-16 | 2022-06-10 | 德默特生物科技(珠海)有限公司 | Hybrid photobioreactor |
Also Published As
| Publication number | Publication date |
|---|---|
| CN112063519B (en) | 2022-06-10 |
| WO2022052418A1 (en) | 2022-03-17 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN112063519B (en) | Photosynthetic bacteria continuous culture device and control method thereof | |
| JP5756482B2 (en) | System, apparatus, and method for cultivating microorganisms and reducing gas | |
| CN101445309B (en) | Ecological fish breeding installation with purified recirculated water | |
| CN1323343A (en) | Photobioreactor | |
| CN207347568U (en) | The Photosynthetic bacteria culturing device of automatic light source | |
| CN100535099C (en) | Biophotosynthetic reactor apparatus and process for producing micro algae | |
| CN201110849Y (en) | Indoor simulating device used for lake sediment-water interface process research | |
| CN106399113A (en) | High density microalgae culture method in membrane photobioreactor using municipal wastewater | |
| CN103864270B (en) | Device and method for treating organic waste water by combining microorganism electrolytic tank and lemna minor cultivation | |
| CN206126956U (en) | A sewage treatment system for northern countryside | |
| CN101172668A (en) | Indoor simulated system applied in lake sediment-water interface process research | |
| CN208648881U (en) | A kind of governing system of rural area black and odorous water and waste | |
| CN208667383U (en) | A kind of integrated sewage treating apparatus | |
| Starovoytov et al. | The Rationale for the Fish Farms Circulating Water Biological Treatment Intensification | |
| CN218478761U (en) | Device capable of regulating and controlling culture of attached algae | |
| EP0214271B1 (en) | Plant for cultivating algae | |
| CN110240998A (en) | The closed bioreactor of the shell and tube of automatic cleaning tube wall | |
| CN218527302U (en) | Agricultural facility factorization indoor eel pond culture device | |
| MX2012004113A (en) | Systems, apparatused and methods for treating wastewater. | |
| CN220224184U (en) | Microorganism culture device in sewage environment | |
| CN213506886U (en) | Microbial cultivation device convenient to temperature detects | |
| CN210168430U (en) | Combined seed culture dish | |
| CN216164497U (en) | Rotifer breeding device for experiments | |
| CN220183029U (en) | Device for continuously treating feces and synchronously producing microalgae | |
| CN220654186U (en) | Seedling breeding device |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |