CN112062292A - Compound bacterium agent for purifying black and odorous water body - Google Patents
Compound bacterium agent for purifying black and odorous water body Download PDFInfo
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 title claims abstract description 86
- 241000894006 Bacteria Species 0.000 title claims abstract description 53
- 239000003795 chemical substances by application Substances 0.000 title claims description 23
- 150000001875 compounds Chemical class 0.000 title description 6
- 239000000843 powder Substances 0.000 claims abstract description 98
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims abstract description 34
- 239000002131 composite material Substances 0.000 claims abstract description 28
- 239000002068 microbial inoculum Substances 0.000 claims abstract description 27
- 230000000243 photosynthetic effect Effects 0.000 claims abstract description 20
- 150000003839 salts Chemical class 0.000 claims abstract description 20
- 239000002994 raw material Substances 0.000 claims abstract description 19
- 241000194108 Bacillus licheniformis Species 0.000 claims abstract description 18
- 241000194107 Bacillus megaterium Species 0.000 claims abstract description 18
- 241000589597 Paracoccus denitrificans Species 0.000 claims abstract description 18
- 244000063299 Bacillus subtilis Species 0.000 claims abstract description 17
- 235000014469 Bacillus subtilis Nutrition 0.000 claims abstract description 17
- 239000004310 lactic acid Substances 0.000 claims abstract description 17
- 235000014655 lactic acid Nutrition 0.000 claims abstract description 17
- 241000193830 Bacillus <bacterium> Species 0.000 claims abstract description 13
- 238000000746 purification Methods 0.000 claims abstract description 13
- 230000000050 nutritive effect Effects 0.000 claims abstract description 4
- 239000007788 liquid Substances 0.000 claims description 43
- 230000001580 bacterial effect Effects 0.000 claims description 33
- 235000015097 nutrients Nutrition 0.000 claims description 16
- 229910052761 rare earth metal Inorganic materials 0.000 claims description 15
- 150000002910 rare earth metals Chemical class 0.000 claims description 15
- 239000002699 waste material Substances 0.000 claims description 15
- 241000196324 Embryophyta Species 0.000 claims description 14
- 239000002351 wastewater Substances 0.000 claims description 13
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 10
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 10
- LWIHDJKSTIGBAC-UHFFFAOYSA-K potassium phosphate Substances [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 claims description 10
- 238000012258 culturing Methods 0.000 claims description 9
- 238000002156 mixing Methods 0.000 claims description 9
- 235000013312 flour Nutrition 0.000 claims description 7
- 241000193755 Bacillus cereus Species 0.000 claims description 6
- 238000002360 preparation method Methods 0.000 claims description 6
- 238000007865 diluting Methods 0.000 claims description 5
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 claims description 5
- 229910000396 dipotassium phosphate Inorganic materials 0.000 claims description 5
- 235000019797 dipotassium phosphate Nutrition 0.000 claims description 5
- 238000004108 freeze drying Methods 0.000 claims description 5
- 238000002386 leaching Methods 0.000 claims description 5
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 5
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 5
- 229940099596 manganese sulfate Drugs 0.000 claims description 5
- 235000007079 manganese sulphate Nutrition 0.000 claims description 5
- 239000011702 manganese sulphate Substances 0.000 claims description 5
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 claims description 5
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 5
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 5
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 claims description 5
- 238000005067 remediation Methods 0.000 claims description 5
- 239000011780 sodium chloride Substances 0.000 claims description 5
- 238000001694 spray drying Methods 0.000 claims description 5
- 235000019733 Fish meal Nutrition 0.000 claims description 4
- 240000007594 Oryza sativa Species 0.000 claims description 4
- 235000007164 Oryza sativa Nutrition 0.000 claims description 4
- 240000008042 Zea mays Species 0.000 claims description 4
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 4
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 4
- 235000005822 corn Nutrition 0.000 claims description 4
- 239000000706 filtrate Substances 0.000 claims description 4
- 238000001914 filtration Methods 0.000 claims description 4
- 239000004467 fishmeal Substances 0.000 claims description 4
- 235000009566 rice Nutrition 0.000 claims description 4
- 241000186660 Lactobacillus Species 0.000 claims description 3
- 229940039696 lactobacillus Drugs 0.000 claims description 3
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- 235000010627 Phaseolus vulgaris Nutrition 0.000 claims description 2
- 244000046052 Phaseolus vulgaris Species 0.000 claims description 2
- 238000001816 cooling Methods 0.000 claims description 2
- 238000010438 heat treatment Methods 0.000 claims description 2
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- QAOWNCQODCNURD-UHFFFAOYSA-N sulfuric acid Substances OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 claims description 2
- 230000000694 effects Effects 0.000 abstract description 15
- 241001052560 Thallis Species 0.000 abstract 1
- 238000000034 method Methods 0.000 description 10
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- 238000005516 engineering process Methods 0.000 description 6
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- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 4
- IOVCWXUNBOPUCH-UHFFFAOYSA-M Nitrite anion Chemical compound [O-]N=O IOVCWXUNBOPUCH-UHFFFAOYSA-M 0.000 description 4
- 230000009286 beneficial effect Effects 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 description 3
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 3
- 238000004140 cleaning Methods 0.000 description 3
- 230000000052 comparative effect Effects 0.000 description 3
- 229910052760 oxygen Inorganic materials 0.000 description 3
- 239000001301 oxygen Substances 0.000 description 3
- 230000008569 process Effects 0.000 description 3
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- 239000004382 Amylase Substances 0.000 description 2
- 102000013142 Amylases Human genes 0.000 description 2
- 108010065511 Amylases Proteins 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 108091005804 Peptidases Proteins 0.000 description 2
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- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 2
- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 description 2
- 235000019418 amylase Nutrition 0.000 description 2
- 230000001651 autotrophic effect Effects 0.000 description 2
- 229940088598 enzyme Drugs 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- 239000002957 persistent organic pollutant Substances 0.000 description 2
- 230000002195 synergetic effect Effects 0.000 description 2
- 108010062877 Bacteriocins Proteins 0.000 description 1
- BVKZGUZCCUSVTD-UHFFFAOYSA-L Carbonate Chemical compound [O-]C([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-L 0.000 description 1
- 108010059892 Cellulase Proteins 0.000 description 1
- 241000195493 Cryptophyta Species 0.000 description 1
- 101710088194 Dehydrogenase Proteins 0.000 description 1
- 102000004882 Lipase Human genes 0.000 description 1
- 239000004367 Lipase Substances 0.000 description 1
- 108090001060 Lipase Proteins 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- OSWRVYBYIGOAEZ-UHFFFAOYSA-N acetic acid;2-hydroxypropanoic acid Chemical compound CC(O)=O.CC(O)C(O)=O OSWRVYBYIGOAEZ-UHFFFAOYSA-N 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- 241001148470 aerobic bacillus Species 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 229940106157 cellulase Drugs 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000005265 energy consumption Methods 0.000 description 1
- 239000003344 environmental pollutant Substances 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 239000010842 industrial wastewater Substances 0.000 description 1
- 229910017053 inorganic salt Inorganic materials 0.000 description 1
- 235000019421 lipase Nutrition 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 244000000010 microbial pathogen Species 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
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- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 231100000719 pollutant Toxicity 0.000 description 1
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- 239000013049 sediment Substances 0.000 description 1
- 230000001953 sensory effect Effects 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 238000004065 wastewater treatment Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/16—Nitrogen compounds, e.g. ammonia
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/16—Nitrogen compounds, e.g. ammonia
- C02F2101/166—Nitrites
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2305/00—Use of specific compounds during water treatment
- C02F2305/06—Nutrients for stimulating the growth of microorganisms
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Microbiology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Hydrology & Water Resources (AREA)
- Engineering & Computer Science (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
- Disinfection, Sterilisation Or Deodorisation Of Air (AREA)
Abstract
The invention relates to a black and odorous water body purification composite microbial inoculum, which comprises the following raw materials in parts by weight: 10-25 parts of bacillus subtilis powder, 6-20 parts of waxy bacillus powder, 10-25 parts of bacillus licheniformis powder, 30-45 parts of paracoccus denitrificans powder, 4-18 parts of lactic acid bacteria powder, 20-35 parts of photosynthetic bacteria powder, 7-12 parts of bacillus megaterium powder and 2-5 parts of nutritive salt. The composite microbial inoculum has strong adaptability and stability, can quickly adapt to severe black and odorous water body environment after being put into use, has good continuous growth of thalli, can achieve good treatment effect 14 days after being put into use, reduces the cost and simultaneously realizes quick and sustainable treatment of the black and odorous water body.
Description
Technical Field
The invention relates to the technical field of wastewater treatment, and particularly relates to a black and odorous water body purification composite microbial inoculum and a preparation method thereof.
Background
Along with the rapid development of the economy of China, the urbanization process is accelerated, the discharge amount of industrial wastewater and domestic sewage is increased year by year, a large amount of sewage is converged into the urban water body, the concentration of pollutants is seriously exceeded, and the phenomenon of seasonal or perennial black and odorous water body is caused, so that the urban black and odorous water body not only brings extremely poor sensory experience to the masses, but also directly influences the production and living water of the masses, eliminates the black and odorous river course of the city, improves the quality of the urban water environment, and has extremely important practical significance for guaranteeing the health of the urban human settlements, and promoting the social harmony and the sustainable.
At present, the treatment technologies for the black and odorous water body of the urban river are numerous, and the biological-ecological restoration technology, the physical restoration technology, the chemical restoration technology and the like are mainly provided, wherein the microbial restoration technology in the biological-ecological restoration technology mainly utilizes the metabolic transformation activity of microbes to degrade organic pollutants so as to achieve the purpose of purifying the water body, and the addition of the microbial agent has the advantages of low energy consumption, small engineering quantity, low cost, good effect, easy operation, long duration, no secondary pollution and the like, and plays an important role in the treatment process of the black and odorous water body of the urban river.
However, the microbial agents commonly used in the market at present are various in types, mostly exogenous microorganisms, expensive in price and mostly unsuitable for specific black and odorous water body environments, organic pollutants in water are difficult to effectively remove after the microbial agents are put in the water, or a large amount of microbial agents are repeatedly put in for a long time to achieve a certain removal effect, the added microbial agents can adapt to the black and odorous water body environments for a long time, and the continuous growth states of the microbial agents are not good.
Disclosure of Invention
The technical problem to be solved by the invention is to provide the black and odorous water purification composite microbial inoculum and the preparation method thereof, so that the adaptation time of the microbial inoculum is shortened, the good state of the microbial inoculum is ensured to be continuously grown, and the black and odorous water is treated with a better effect.
The technical problem solved by the invention is realized by adopting the following technical scheme:
the compound microbial inoculum for purifying the black and odorous water body comprises the following raw materials in parts by weight: 10-25 parts of bacillus subtilis powder, 6-20 parts of waxy bacillus powder, 10-25 parts of bacillus licheniformis powder, 30-45 parts of paracoccus denitrificans powder, 4-18 parts of lactic acid bacteria powder, 20-35 parts of photosynthetic bacteria powder, 7-12 parts of bacillus megaterium powder and 2-5 parts of nutritive salt.
Preferably, the bacterial powder is prepared by mixing corresponding bacterial liquid and a carrier according to the mass ratio of 1: 1-10, culturing for 2-5 days, and freeze-drying or spray-drying, and the bacterial liquid is obtained by separating, purifying, expanding, culturing and diluting black and odorous wastewater or black and odorous water demonstration remediation area wastewater.
Further preferably, the target viable cell count of each bacterial powder is: bacillus subtilis liquid 5-20 × 109CFU/g, bacillus cereus 5-15 multiplied by 109CFU/g, 6-25 multiplied by 10 bacillus licheniformis liquid9CFU/g, 5-25 x 10 paracoccus denitrificans liquid9CFU/g, 3-15 multiplied by 10 lactobacillus liquid9CFU/g, 6-35 multiplied by 10 photosynthetic bacteria liquid9CFU/g, bacillus megaterium liquid 5-10 multiplied by 109CFU/g。
Further preferably, the carrier is formed by mixing one or more of rice bran, corn flour, wheat bran, fish meal and bean flour.
Further preferably, the complex microbial inoculum consists of the following raw materials in parts by weight: 16 parts of bacillus subtilis powder, 8 parts of waxy bacillus powder, 17 parts of bacillus licheniformis powder, 30 parts of paracoccus denitrificans powder, 7 parts of lactic acid bacteria powder, 21 parts of photosynthetic bacteria powder, 5 parts of bacillus megaterium powder and 2 parts of nutrient salt.
Further preferably, the complex microbial inoculum consists of the following raw materials in parts by weight: 25 parts of bacillus subtilis powder, 10 parts of waxy bacillus powder, 25 parts of bacillus licheniformis powder, 45 parts of paracoccus denitrificans powder, 8 parts of lactic acid bacteria powder, 25 parts of photosynthetic bacteria powder, 8 parts of bacillus megaterium powder and 3 parts of nutrient salt.
Further preferably, the complex microbial inoculum consists of the following raw materials in parts by weight: 25 parts of bacillus subtilis powder, 15 parts of waxy bacillus powder, 17 parts of bacillus licheniformis powder, 30 parts of paracoccus denitrificans powder, 15 parts of lactic acid bacteria powder, 35 parts of photosynthetic bacteria powder, 10 parts of bacillus megaterium powder and 5 parts of nutrient salt.
Further preferably, the nutrient salt comprises the following raw materials in parts by weight: 10-30 parts of brown sugar, 0.1-0.5 part of monopotassium phosphate, 0.2-1 part of dipotassium phosphate, 0.01-0.1 part of magnesium sulfate, 0.001-0.1 part of manganese sulfate, 1-5 parts of sodium chloride, 0.01-0.08 part of plant ash leachate and 0.001-0.01 part of rare earth waste residue leachate.
Further preferably, the preparation method of the plant ash leachate comprises the following steps: mixing plant ash and water according to a mass ratio of 1:50, oscillating for 1h at 180r/min, standing for 30min, and filtering by 8 layers of gauze to obtain a filtrate, namely a plant ash leaching solution.
Further preferably, the preparation method of the rare earth waste residue leaching solution comprises the following steps: heating a concentrated sulfuric acid solution with the concentration of 98% to 100 ℃, slowly adding the rare earth waste residue while stirring, preserving the temperature for 30-60 min, cooling to room temperature, and filtering to obtain filtrate, namely the rare earth waste residue leaching solution.
The plant ash leachate is used for providing inorganic salt for the growth supplement of microorganisms, sulfate and trace elements contained in the rare earth waste residue leachate, and the rare earth waste residue leachate is used as an acid leachate and can be used for adjusting the pH value of a culture solution, so that the good state growth of the compound bacteria is comprehensively promoted.
Under the mass ratio and viable bacteria ratio, the enzymes generated by each strain can rapidly decompose organic matters in water and bottom sediment, and have good removal effect on ammonia nitrogen, nitrite and COD, and the generated components of the nourishing components such as the organic acid, the physiologically active substances and the like and nutritive salt have good synergistic effect to promote the growth of each strain, ensure that each strain can rapidly adapt to the severe water environment, ensure the good and continuous growth of the strain, reduce the growth of pathogenic microorganisms and harmful algae by lactic acid bacteria, and can live in the water environment and play a role of dominant flora to maintain the microecological balance of the water and improve the water quality; the bacillus megaterium has the capability of reducing ammonia nitrogen, nitrite and denitrification of water, can effectively remove nitrogen in the water, and has a certain effect of removing total phosphorus in the water; the photosynthetic bacteria can grow in an autotrophic manner under the illumination condition, and can be symbiotic with other bacteria, the degradation activity of the photosynthetic bacteria is not limited by the oxygen concentration of a water body, and harmful substances such as nitrite, carbonate, sulfate and the like in the water body and bottom mud can be greatly degraded, so that the effects of purifying water quality, increasing dissolved oxygen and the like are realized; the bacillus licheniformis can secrete various enzymes such as amylase, protease, acetate-lactate dehydrogenase and the like, can also secrete some bacteriocin antibacterial substances, inhibits the growth and the propagation of harmful bacteria, and can effectively improve the water quality of a water body under the synergistic action of bacillus, photosynthetic bacteria and other bacteria, thereby being beneficial to the growth and the propagation of aquatic organisms, better repairing and maintaining the microecological balance of the water body; the bacillus cereus is aerobic bacteria, can rapidly consume free oxygen in the environment, can synthesize amylase, protease, lipase, cellulase and the like, realizes proliferation growth, can effectively remove ammonia nitrogen and nitrite in water, and further improves the water environment; paracoccus denitrificans is facultative inorganic autotrophic bacteria, can survive and grow under aerobic or anoxic conditions, and has good conversion and removal effects on inorganic nitrogen in water.
Has the advantages that: the black and odorous water body purification composite microbial inoculum can quickly adapt to a severe black and odorous water body environment after being put into the tank, the microbial inoculum grows well continuously, a good treatment effect can be achieved after the tank is put into the tank for 14 days, the cost is reduced, and meanwhile, the quick and sustainable treatment of the black and odorous water body is realized.
Detailed Description
In order to make the technical means, the creation features, the achievement purposes and the effects of the invention easy to understand, the invention is further described with the specific embodiments.
Example 1
The complex microbial inoculum described in the embodiment is prepared from the following componentsThe raw materials in parts by weight are as follows: 16 parts of bacillus subtilis powder, 8 parts of waxy bacillus powder, 17 parts of bacillus licheniformis powder, 30 parts of paracoccus denitrificans powder, 7 parts of lactic acid bacteria powder, 21 parts of photosynthetic bacteria powder, 5 parts of bacillus megaterium powder and 2 parts of nutrient salt, wherein each bacteria powder is prepared by mixing corresponding bacteria liquid and a carrier according to a mass ratio of 1:5, culturing for 1-3 days and then freeze-drying or spray-drying, and the bacteria liquid is obtained by separating, purifying, expanding culture and diluting from black and odorous wastewater or black and odorous water demonstration and remediation area wastewater, and the target viable count of each bacteria liquid is as follows: bacillus subtilis liquid 9X 109CFU/g, Bacillus cereus 8X 109CFU/g, Bacillus licheniformis liquid 17X 109CFU/g, paracoccus denitrificans liquid 10 x 109CFU/g, 7X 10 lactobacillus liquid9CFU/g, photosynthetic bacteria liquid 21X 109CFU/g, Bacillus megaterium liquid 5X 109CFU/g。
In this example, the carrier is a mixture of rice bran and corn flour.
The nutrient salt is prepared from the following raw materials in parts by weight: 30 parts of brown sugar, 0.5 part of monopotassium phosphate, 1 part of dipotassium phosphate, 0.01 part of magnesium sulfate, 0.1 part of manganese sulfate, 5 parts of sodium chloride, 0.01 part of plant ash leachate and 0.01 part of rare earth waste residue leachate
The application method of the embodiment is as follows: the method is implemented in a demonstration and restoration area of a certain black and odorous water body in Hunan province, the data before and after the experiment are shown in Table 1, the composite microbial inoculum is uniformly added into the black and odorous water body, and the adding amount is 20-50 g per cubic meter of water; after the composite microbial agent is continuously added for 7 days, the removal rates of TP and TN in the water body in the black and odorous lake channel restoration demonstration area reach 39.69 percent and 57.95 percent respectively; after the continuous addition for 14 days, the removal rates of TP and TN in the water body of the black and odorous lake channel restoration demonstration area reach 67.98 percent and 79.23 percent respectively; the microbial inoculum is added, so that the growth of beneficial microorganisms in the water body can be effectively promoted, the restoration of the micro-ecological environment of the river channel is facilitated, and the self-cleaning capacity of the water body can be enhanced.
TABLE 1 comparison of the Water quality tables (mg/L) of the composite bacterial agent treatment described in example 1
Example 2
The composite microbial inoculum described in the embodiment comprises the following raw materials in parts by weight: the compound microbial inoculum is composed of the following raw materials in parts by weight: 25 parts of bacillus subtilis powder, 10 parts of waxy bacillus powder, 25 parts of bacillus licheniformis powder, 45 parts of paracoccus denitrificans powder, 8 parts of lactic acid bacteria powder, 25 parts of photosynthetic bacteria powder, 8 parts of bacillus megaterium powder and 3 parts of nutrient salt. The bacterial powder is prepared by mixing corresponding bacterial liquid and a carrier according to the mass ratio of 1:10, culturing for 1-3 days, and then freeze-drying or spray-drying, wherein the bacterial liquid is obtained by separating, purifying, expanding, culturing and diluting black and odorous wastewater or black and odorous water demonstration and remediation area wastewater, and the target viable count of each bacterial liquid is as follows: bacillus subtilis liquid 20X 109CFU/g, Bacillus cereus 10X 109CFU/g, Bacillus licheniformis liquid 25X 109CFU/g, paracoccus denitrificans liquid 25X 109CFU/g, 8X 10 lactic acid bacteria liquid9CFU/g, photosynthetic bacteria liquid 25X 109CFU/g, Bacillus megaterium liquid 8X 109CFU/g。
In this example, the carrier is a mixture of rice bran, corn flour, wheat bran, and fish meal.
The nutrient salt is prepared from the following raw materials in parts by weight: 10 parts of brown sugar, 0.1 part of monopotassium phosphate, 0.2 part of dipotassium phosphate, 0.01 part of magnesium sulfate, 0.1 part of manganese sulfate, 1 part of sodium chloride, 0.08 part of plant ash leachate and 0.001 part of rare earth waste residue leachate.
The application method of the embodiment is as follows: the method is implemented in a demonstration and restoration area of a certain black and odorous water body in Hunan province, the data before experiments are shown in Table 2, the composite microbial inoculum is uniformly added into the black and odorous water body, and the adding amount is 20-50 g per cubic meter of water; after the composite microbial agent is continuously added for 7 days, the removal rates of TP and TN in the water body in the black and odorous lake channel restoration demonstration area reach 40.63% and 60.59% respectively; after the continuous addition for 14 days, the removal rates of TP and TN in the water body of the black and odorous lake channel restoration demonstration area reach 72.86 percent and 83.38 percent respectively; the microbial inoculum is added, so that the growth of beneficial microorganisms in the water body can be effectively promoted, the restoration of the micro-ecological environment of the river channel is facilitated, and the self-cleaning capacity of the water body can be enhanced.
TABLE 2 comparison of the Water quality tables (mg/L) of the composite bacterial agent treatment described in example 2
Example 3
The composite microbial inoculum described in the embodiment comprises the following raw materials in parts by weight: the compound microbial inoculum is composed of the following raw materials in parts by weight: 25 parts of bacillus subtilis powder, 15 parts of waxy bacillus powder, 17 parts of bacillus licheniformis powder, 30 parts of paracoccus denitrificans powder, 15 parts of lactic acid bacteria powder, 35 parts of photosynthetic bacteria powder, 10 parts of bacillus megaterium powder and 5 parts of nutrient salt. The bacterial powder is prepared by mixing corresponding bacterial liquid and a carrier according to the mass ratio of 1:1, culturing for 1-3 days, and then freeze-drying or spray-drying, wherein the bacterial liquid is obtained by separating, purifying, expanding, culturing and diluting black and odorous wastewater or black and odorous water demonstration and remediation area wastewater, and the target viable count of each bacterial liquid is as follows: bacillus subtilis liquid 20X 109CFU/g, Bacillus cereus 15X 109CFU/g, Bacillus licheniformis liquid 17X 109CFU/g, paracoccus denitrificans liquid 10 x 109CFU/g, lactic acid bacteria liquid 15X 109CFU/g, photosynthetic bacteria liquid 35X 109CFU/g, Bacillus megaterium liquid 10X 109CFU/g。
In this example, the carrier is a mixture of fish meal and soy flour.
The nutrient salt is prepared from the following raw materials in parts by weight: 15 parts of brown sugar, 0.4 part of monopotassium phosphate, 0.8 part of dipotassium phosphate, 0.05 part of magnesium sulfate, 0.05 part of manganese sulfate, 3 parts of sodium chloride, 0.06 part of plant ash leachate and 0.009 parts of rare earth waste residue leachate.
The application method of the embodiment is as follows: the method is implemented in a demonstration and restoration area of a certain black and odorous water body in Hunan province, the data before and after the experiment are shown in Table 3, the composite microbial inoculum is uniformly added into the black and odorous water body, and the adding amount is 20-50 g per cubic meter of water; after the composite microbial agent is continuously added for 7 days, the removal rates of TP and TN in the water body in the black and odorous lake channel restoration demonstration area reach 42.76% and 62.49% respectively; after the continuous addition for 14 days, the removal rates of TP and TN in the water body of the black and odorous lake channel restoration demonstration area reach 80% and 86.31% respectively; the microbial inoculum is added, so that the growth of beneficial microorganisms in the water body can be effectively promoted, the restoration of the micro-ecological environment of the river channel is facilitated, and the self-cleaning capacity of the water body can be enhanced.
TABLE 3 comparison of the Water quality tables (mg/L) of the composite bacterial agent treatment described in example 3
| Index (I) | TP | TN | DO | Transparency (cm) |
| Before treatment | 0.725 | 12.05 | 1.5 | 25 |
| After 7 days of treatment | 0.415 | 4.52 | 3.6 | 34 |
| After 14 days of treatment | 0.145 | 1.65 | 5.2 | 75 |
Comparative example 1
The bacterial powders were commercially available, and the target viable bacterial ratios described in example 1 were prepared from the commercially available bacterial powders according to the mass ratios described in example 1, and the same wastewater was dosed as described in example 1, and the data before and after the test are shown in table 4, which indicates that the effect is not satisfactory when using the commercially available bacterial powders.
TABLE 4 comparison of Water quality tables (mg/L) for Complex microbial inoculum treatment described in example 4
| Index (I) | TP | TN | DO | Transparency (cm) |
| Before treatment | 0.456 | 9.87 | 1.8 | 15 |
| After 7 days of treatment | 0.325 | 4.65 | 2.2 | 20 |
| After 14 days of treatment | 0.246 | 3.14 | 4.1 | 52 |
Comparative example 2
Compared with the mixture ratio of the embodiment 2, one component of the lactic acid bacteria powder is reduced, other components are the same as the mixture ratio, the target viable count is consistent, other bacteria powder except the lactic acid bacteria powder is mixed and then put into the same wastewater according to the method described in the embodiment 2, the data before and after the test is shown in the table 5, the applicant also fully tests experiments of lacking and increasing components in the test process, the effect is not ideal as the formula combination described in the invention, and the formula ratio of the composite bacteria is very important.
TABLE 5 comparison of Water quality tables (mg/L) of Complex microbial inoculum treatment described in example 5
| Index (I) | TP | TN | DO | Transparency (cm) |
| Before treatment | 0.608 | 10.05 | 2.1 | 20 |
| After 7 days of treatment | 0.425 | 6.96 | 3.2 | 30 |
| After 14 days of treatment | 0.216 | 3.67 | 4.5 | 58 |
Comparative example 3
Compared with the mixture ratio of the embodiment 3, the difference is that the plant ash leachate and the rare earth waste residue leachate in the nutrient salt are replaced by clear water, the same wastewater is put into the device in the embodiment 3 after the bacterial powder is prepared, the data before and after the test are shown in the table 6, and the test proves that the plant ash leachate and the rare earth waste residue leachate in the nutrient salt play an important role in the good-state growth of the composite bacteria and have larger influence on the final sewage treatment effect.
TABLE 6 comparison of Water quality tables (mg/L) for Complex microbial inoculum treatment described in example 6
The foregoing shows and describes the general principles, essential features, and advantages of the invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are described in the specification and illustrated only to illustrate the principle of the present invention, but that various changes and modifications may be made therein without departing from the spirit and scope of the present invention, which fall within the scope of the invention as claimed. The scope of the invention is defined by the appended claims and equivalents thereof.
Claims (10)
1. The black and odorous water body purification composite bacterial agent is characterized by comprising the following raw materials in parts by weight: 10-25 parts of bacillus subtilis powder, 6-20 parts of waxy bacillus powder, 10-25 parts of bacillus licheniformis powder, 30-45 parts of paracoccus denitrificans powder, 4-18 parts of lactic acid bacteria powder, 20-35 parts of photosynthetic bacteria powder, 7-12 parts of bacillus megaterium powder and 2-5 parts of nutritive salt.
2. The black and odorous water purification composite bacterial agent according to claim 1, wherein each bacterial powder is prepared by mixing corresponding bacterial liquid and a carrier in a mass ratio of 1: 1-10, culturing for 1-3 days, and freeze-drying or spray-drying, and the bacterial liquid is obtained by separating, purifying, expanding, culturing and diluting the black and odorous wastewater or black and odorous water demonstration remediation area wastewater.
3. The black and odorous water body purification composite bacterial agent according to claim 1, wherein the number of target viable bacteria of each bacterial powder is: bacillus subtilis liquid 5-20 × 109CFU/g, bacillus cereus 5-15 multiplied by 109CFU/g, 6-25 multiplied by 10 bacillus licheniformis liquid9CFU/g, 5-25 x 10 paracoccus denitrificans liquid9CFU/g, 3-15 multiplied by 10 lactobacillus liquid9CFU/g, 6-35 multiplied by 10 photosynthetic bacteria liquid9CFU/g, bacillus megaterium liquid 5-10 multiplied by 109CFU/g。
4. The black odorous water body purification composite bacterial agent as claimed in claim 2, wherein the carrier is formed by mixing one or more of rice bran, corn flour, wheat bran, fish meal and bean flour.
5. The black and odorous water body purification composite bacterial agent according to claim 1, which is prepared from the following raw materials in parts by weight: 16 parts of bacillus subtilis powder, 8 parts of waxy bacillus powder, 17 parts of bacillus licheniformis powder, 30 parts of paracoccus denitrificans powder, 7 parts of lactic acid bacteria powder, 21 parts of photosynthetic bacteria powder, 5 parts of bacillus megaterium powder and 2 parts of nutrient salt.
6. The black and odorous water body purification composite bacterial agent according to claim 1, which is prepared from the following raw materials in parts by weight: 25 parts of bacillus subtilis powder, 10 parts of waxy bacillus powder, 25 parts of bacillus licheniformis powder, 45 parts of paracoccus denitrificans powder, 8 parts of lactic acid bacteria powder, 25 parts of photosynthetic bacteria powder, 8 parts of bacillus megaterium powder and 3 parts of nutrient salt.
7. The black and odorous water body purification composite bacterial agent according to claim 1, which is prepared from the following raw materials in parts by weight: 25 parts of bacillus subtilis powder, 15 parts of waxy bacillus powder, 17 parts of bacillus licheniformis powder, 30 parts of paracoccus denitrificans powder, 15 parts of lactic acid bacteria powder, 35 parts of photosynthetic bacteria powder, 10 parts of bacillus megaterium powder and 5 parts of nutrient salt.
8. The black and odorous water body purification composite bacterial agent according to claim 1, wherein the nutrient salt is composed of the following raw materials in parts by weight: 10-30 parts of brown sugar, 0.1-0.5 part of monopotassium phosphate, 0.2-1 part of dipotassium phosphate, 0.01-0.1 part of magnesium sulfate, 0.001-0.1 part of manganese sulfate, 1-5 parts of sodium chloride, 0.01-0.08 part of plant ash leachate and 0.001-0.01 part of rare earth waste residue leachate.
9. The black odorous water body purification composite bacterial agent according to claim 8, wherein the preparation method of the plant ash leachate comprises the following steps: mixing plant ash and water according to a mass ratio of 1:50, oscillating for 1h at 180r/min, standing for 30min, and filtering by 8 layers of gauze to obtain a filtrate, namely a plant ash leaching solution.
10. The composite microbial inoculum for purifying black and odorous water bodies according to claim 8, wherein the preparation method of the rare earth waste residue leachate comprises the following steps: heating a concentrated sulfuric acid solution with the concentration of 98% to 100 ℃, slowly adding the rare earth waste residue while stirring, preserving the temperature for 30-60 min, cooling to room temperature, and filtering to obtain filtrate, namely the rare earth waste residue leaching solution.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105400723A (en) * | 2015-12-18 | 2016-03-16 | 杭州楠溪生态环境科技有限公司 | Composite microbial preparation used for river treatment |
| CN106754514A (en) * | 2016-12-23 | 2017-05-31 | 钟华 | A kind of sustainable desilting is except the microbial bacterial agent preparation method of black and odorous water |
| CN107188741A (en) * | 2017-08-02 | 2017-09-22 | 合肥百绿盛农业科技有限公司 | A kind of preparation method of fertilizer for flower planting |
| CN108928929A (en) * | 2018-05-25 | 2018-12-04 | 宁波市川宁环保科技有限公司 | Microorganism formulation for black and odorous water purification |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105400723A (en) * | 2015-12-18 | 2016-03-16 | 杭州楠溪生态环境科技有限公司 | Composite microbial preparation used for river treatment |
| CN106754514A (en) * | 2016-12-23 | 2017-05-31 | 钟华 | A kind of sustainable desilting is except the microbial bacterial agent preparation method of black and odorous water |
| CN107188741A (en) * | 2017-08-02 | 2017-09-22 | 合肥百绿盛农业科技有限公司 | A kind of preparation method of fertilizer for flower planting |
| CN108928929A (en) * | 2018-05-25 | 2018-12-04 | 宁波市川宁环保科技有限公司 | Microorganism formulation for black and odorous water purification |
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