CN112056482A - Sleep-aiding small-molecule peptide solid beverage and preparation method and application thereof - Google Patents

Sleep-aiding small-molecule peptide solid beverage and preparation method and application thereof Download PDF

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CN112056482A
CN112056482A CN202011091522.9A CN202011091522A CN112056482A CN 112056482 A CN112056482 A CN 112056482A CN 202011091522 A CN202011091522 A CN 202011091522A CN 112056482 A CN112056482 A CN 112056482A
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sleep
aiding
oligopeptide
vitamin
solid beverage
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周录泳
孔小军
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Guangzhou Xiaoxiang Health Industry Co ltd
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Abstract

The invention relates to a sleep-aiding small molecular peptide solid beverage and a preparation method and application thereof. The sleep-aiding small molecular peptide solid beverage comprises sturgeon skin oligopeptide, wheat oligopeptide, pecan oligopeptide, cow milk oligopeptide, gamma-aminobutyric acid, N-acetylneuraminic acid and compound vitamins. The sleep-aiding small molecular peptide solid beverage provided by the invention is green and natural in raw materials, fine and mellow in taste, and has an obvious sleep-aiding effect, the sleep latency time can be greatly shortened, the sleep validity time can be prolonged, and the defects of unobvious efficacy and large side effect of the existing sleep-aiding products are overcome to a great extent.

Description

Sleep-aiding small-molecule peptide solid beverage and preparation method and application thereof
Technical Field
The invention belongs to the field of functional foods or health-care products, and particularly relates to a sleep-aiding small-molecular-peptide solid beverage as well as a preparation method and application thereof.
Background
With the rapid development of social economy, the pace of life is accelerated continuously, the pressure from the society, life and work is increased, and people in sub-health state are more and more, so that a plurality of unstable and discordant potential factors are brought to individuals, families and society, and the social productivity and the life quality of people are seriously influenced. Among them, insomnia and the related symptoms caused by insomnia are more common and prominent in many sub-health states, and are receiving more and more attention.
Insomnia is a disease characterized by frequent failure to obtain normal sleep, and is mainly characterized by short sleep time, insufficient deep sleep, difficulty in sleeping for light people, difficulty in sleeping for sleeping without soundness, incapability of sleeping when sleeping or after waking, and insomnia for heavy people all over night. Insomnia has now become the second largest disease in neurology clinics following headache.
At present, the medicines for treating insomnia are mainly western medicines and partial traditional Chinese medicines, and the medicines have the problems of inaccurate effect, long-term administration, immediate attack after medicine withdrawal, high possibility of generating dependence, high toxic and side effects and the like. The long-term taking of the medicine is harmful to the physical health of patients and even causes other iatrogenic diseases. Therefore, the development of safe and effective novel sleep-aiding medicines, health-care products or functional foods is inevitably a development trend in the field, attention is paid to the adoption of dietary therapy for relieving or eradicating certain diseases including insomnia in recent years, and no satisfactory dietary therapy scheme or product exists on the market so far.
The patent CN104738624A discloses a nutritional health food for neurasthenia, amnesia and insomnia, which is prepared by matching medical and edible Chinese herbal medicines and is added with short peptides, amino acids, carbohydrates, multiple vitamins and multiple mineral substances. The formula is added with traditional Chinese medicines, and is not suitable for long-term administration.
Therefore, the development of the sleep-aiding product with remarkable sleep-aiding effect and small side effect has important research significance and popularization value.
Disclosure of Invention
The invention aims to overcome the defect of food therapy schemes or products with obvious sleep-aiding effect in the prior art, and provides a sleep-aiding small molecular peptide solid beverage. The sleep-aiding small molecular peptide solid beverage provided by the invention takes compatibility of various small molecular peptides as a main material, and is supplemented with neurotrophic factors such as gamma-aminobutyric acid, N-acetylneuraminic acid, compound vitamins and the like, so that the raw materials are green and natural, the taste is fine and mellow, the sleep-aiding small molecular peptide solid beverage has an obvious sleep-aiding effect, and the defects of unobvious efficacy and great side effect of the existing sleep-aiding products are overcome to a great extent; and the preparation is in the form of a solid preparation, so that the problems that related liquid preparations are difficult to store and carry and the like are avoided.
The invention also aims to provide a preparation method of the sleep-aiding small-molecule peptide solid beverage.
The invention also aims to provide application of the sleep-aiding small molecular peptide solid beverage in preparation of sleep-aiding, nutrition-repairing, organism immunity-improving or cosmetic medicines, health-care products or functional foods or preparations.
In order to achieve the purpose, the invention adopts the following technical scheme:
the sleep-aiding small molecular peptide solid beverage comprises the following main materials and auxiliary materials in parts by weight:
main materials: 10-15 parts of sturgeon skin oligopeptide;
30-40 parts of wheat oligopeptide;
30-40 parts of pecan oligopeptide;
20-30 parts of cow milk oligopeptide;
auxiliary materials: 1-5 parts of gamma-aminobutyric acid;
1 to 5 parts of N-acetylneuraminic acid;
the addition amount of the compound vitamin is within the standard addition amount range of the national standard food additive.
The causes of sleep disorders include various factors, such as sleep mood, physical and mental fatigue, synthesis and secretion deficiency of sleep factors, and the like. From the several aspects, the obtained multi-angle sleep-assisting product has a better sleep-assisting effect and a wider application range.
On one hand, the wheat oligopeptide can promote the repair of damaged cells of skeletal muscle, maintain the integrity of the cells of the skeletal muscle, reduce the generation of oxygen free radicals of cardiac mitochondria, maintain the normal structure and function of the cardiac mitochondria, protect the heart and improve the oxygen resistance; on the other hand, the wheat oligopeptide rich in homoglutamine can effectively regulate nerves. Thereby promoting sleep by reducing physical and mental fatigue.
Tryptophan in the pecan oligopeptide can promote the secretion of serotonin and melatonin, and glutamic acid can promote the synthesis of inhibitory neurotransmitters such as serotonin and gamma-aminobutyric acid in brain, thereby playing a positive role in improving sleep.
Tryptophan rich in sturgeon skin oligopeptide and cow milk oligopeptide is a main raw material for synthesizing 5-hydroxytryptamine by brain, and can improve the mood before sleep, inhibit nerve activity and shorten sleep latency.
Repeated researches show that the specific plant small molecular peptide: wheat and hickory oligopeptides, and specific animal small molecule peptides: the sturgeon skin oligopeptide and the cow milk oligopeptide are compounded, so that nerve cells can be well nourished, and the sturgeon skin oligopeptide participates in repairing and improving brain cell metabolism; the gamma-aminobutyric acid has the functions of calming, helping sleep and resisting anxiety; n-acetylneuraminic acid has the functions of participating in maintaining the structural and functional integrity of cerebral nerves, promoting the differentiation, development, regeneration, synaptic transmission and the like of nerve cells; vitamins can be involved in maintaining the health of the nervous system.
The special micromolecule peptide composition is used as the main material, so that a relatively comprehensive sleep-aiding effect can be cooperatively exerted from the aspects of improving sleep emotion, reducing physical and mental fatigue, increasing synthesis and secretion of sleep factors and the like, and the phenomenon of poor sleep-aiding effect caused by single component is reduced; meanwhile, functional neurotrophic factors are used as auxiliary materials, and the main materials and the auxiliary materials are compounded to obtain the sleep-aiding small molecular peptide solid beverage, which not only has green and natural raw materials and fine and mellow taste, but also has obvious sleep-aiding effect, can greatly shorten the sleep latency time and prolong the sleep effective period time, and overcomes the defects of unobvious efficacy and great side effect of the existing sleep-aiding products to a great extent; and the preparation is in the form of a solid preparation, so that the problems that related liquid preparations are difficult to store and carry and the like are avoided.
Preferably, the sleep-aiding small molecular peptide solid beverage comprises the following main materials and auxiliary materials in parts by weight:
main materials: 13-15 parts of sturgeon skin oligopeptide;
30-35 parts of wheat oligopeptide;
32-35 parts of pecan oligopeptide;
25-28 parts of cow milk oligopeptide;
auxiliary materials: 2-3 parts of gamma-aminobutyric acid;
2 to 3 parts of N-acetylneuraminic acid;
the addition amount of the compound vitamin is as follows: the addition amount of the vitamin A is 500-800 mu g RAE/100 g; the addition amount of the vitamin C is 100-200 mg/100 g; the addition amount of the vitamin D is 1.5-5.0 mu g/100 g; the addition amount of the vitamin E is 5.0-20 mg of alpha-TE/100 g; in the B vitamins, the addition amount of vitamin B1 is 0.5-5.0 mg/100g, the addition amount of vitamin B2 is 0.5-5.0 mg/100g, the addition amount of vitamin B6 is 0.5-5.0 mg/100g, and the addition amount of vitamin B12 is 1.0-5.0 mg/100 g.
The sturgeon skin oligopeptide, the wheat oligopeptide, the hickory oligopeptide and the cow milk oligopeptide provided by the invention can be prepared by the following methods.
Preferably, the sturgeon skin oligopeptide is prepared by the following steps:
s11: soaking sturgeon skin, and homogenizing to obtain sturgeon skin homogenate;
s12: adding alkaline protease into the sturgeon skin homogenate, carrying out enzymolysis for 1-2 h at the temperature of 45-55 ℃, inactivating, filtering, and centrifuging to obtain the sturgeon skin oligopeptide.
Preferably, the wheat oligopeptide is prepared by the following steps:
s21: soaking wheat, and homogenizing to obtain wheat homogenate;
s22: and adding alkaline protease into the wheat homogenate, carrying out enzymolysis for 1-2 h at the temperature of 45-55 ℃, inactivating, filtering, and centrifuging to obtain the wheat oligopeptide.
Preferably, the pecan oligopeptide is prepared by the following steps:
s31: soaking hickory nuts and then homogenizing to obtain hickory nut homogenate;
s32: and adding alkaline protease into the hickory nut homogenate, carrying out enzymolysis for 1-2 h at the temperature of 45-55 ℃, inactivating, filtering, and centrifuging to obtain the hickory nut oligopeptide.
Preferably, the cow milk oligopeptide is prepared by the following process: adding alkaline protease into raw milk, carrying out enzymolysis for 1-2 h at the temperature of 45-55 ℃, inactivating, filtering, and centrifuging to obtain the milk oligopeptide.
Preferably, the multivitamins include at least two of the vitamin A, B family of vitamins, vitamin C, and vitamin E.
More preferably, the compound vitamin comprises vitamin A, B complex vitamin, vitamin C and vitamin E. Adequate and comprehensive vitamins can better participate in maintaining the health of the nervous system.
It should be noted that the addition of vitamins is performed according to the standard addition of national standard food additives. The addition amount of the vitamin A is 250-800 mu g RAE/100 g; the addition amount of the vitamin C is 30-500 mg/100 g; the addition amount of the vitamin D is 1.5-10 mu g/100 g; the addition amount of the vitamin E is 5.0-150 mg of alpha-TE/100 g; in the B vitamins, the addition amount of vitamin B1 is 0.5-20 mg/100g, the addition amount of vitamin B2 is 0.5-20 mg/100g, the addition amount of vitamin B6 is 0.5-10 mg/100g, and the addition amount of vitamin B12 is 1.0-10 mg/100 g.
The preparation method of the sleep-aiding small molecular peptide solid beverage comprises the following steps: and mixing sturgeon skin oligopeptide, wheat oligopeptide, pecan oligopeptide, cow milk oligopeptide, gamma-aminobutyric acid, N-acetylneuraminic acid and compound vitamin, and spray-drying to obtain the sleep-aiding small molecular peptide solid beverage.
The preparation method provided by the invention is simple in process, green and environment-friendly, and easy to popularize and produce in a large scale.
The sleep-aiding small molecular peptide solid beverage provided by the invention is easily redissolved and reduced when meeting water, and is beneficial to digestion and absorption; easy to store and carry, suitable for the present fast-paced life style; has higher social benefit and economic benefit.
Preferably, spray drying is carried out to a moisture content of not more than 5%.
The sleep-aiding small molecular peptide solid beverage provided by the invention can be further sterilized and vacuum-packaged for better storage and transportation.
The application of the sleep-aiding small molecular peptide solid beverage in preparing sleep-aiding, nutrition-repairing, body immunity-improving or cosmetic medicines, health-care products or functional foods or preparations is also within the protection scope of the invention.
Preferably, the sleep-aiding small molecular peptide solid beverage is applied to preparation of preparations for shortening sleep latency time and prolonging sleep valid period time.
Compared with the prior art, the invention has the following beneficial effects:
the specific micromolecular peptide composition is used as the main material, the functional neurotrophic factor is used as the auxiliary material, and the sleep-aiding micromolecular peptide solid beverage obtained by compounding the main material and the auxiliary material has green and natural raw materials, fine and mellow taste and obvious sleep-aiding effect, can greatly shorten the sleep latency time and prolong the sleep effective period time, and overcomes the defects of unobvious efficacy and great side effect of the existing sleep-aiding product to a great extent.
The preparation method provided by the invention is simple in process, green and environment-friendly, and easy to popularize and produce in a large scale; the sleep-aiding small molecular peptide solid beverage is easily redissolved and reduced when meeting water, and is beneficial to digestion and absorption; easy to store and carry, suitable for the current fast-paced life style and has higher social and economic benefits.
Detailed Description
The invention is further illustrated by the following examples. These examples are intended to illustrate the invention and are not intended to limit the scope of the invention. Experimental procedures without specific conditions noted in the examples below, generally according to conditions conventional in the art or as suggested by the manufacturer; the raw materials, reagents and the like used are, unless otherwise specified, those commercially available from the conventional markets and the like. Any insubstantial changes and substitutions made by those skilled in the art based on the present invention are intended to be covered by the claims.
The sturgeon skin oligopeptide, the wheat oligopeptide, the pecan oligopeptide and the cow milk oligopeptide provided by the embodiments of the invention can be prepared by the following methods.
(1) And respectively adding 2-3 times of clear water into the washed wheat, soaking for 20-30 minutes, and homogenizing to obtain the wheat homogenate liquid. And then adding alkaline protease (papain: trypsin is 1: 1, and the enzyme activity is 40-50 ten thousand u/g) with the mass of 0.3-0.5% of the raw material into the corresponding homogenate, carrying out enzymolysis for 1-2 h at the temperature of 45-55 ℃, and after enzymolysis, adjusting the temperature to 85-90 ℃ to inactivate the enzyme for 15-20 min. Filtering and centrifuging the enzymolysis liquid, and taking supernate to carry out ultrafiltration and centrifugation (the cut-off molecular weight is 30ku) on the micromolecule peptide. Specifically, the mass of the added alkaline protease is 0.3 percent of the mass of the raw materials, the enzymolysis temperature is 45 ℃, and the enzymolysis is carried out for 2 hours.
(2) And respectively adding 2-3 times of clear water into the washed hickory nuts, soaking for 20-30 minutes, and homogenizing to obtain walnut homogenate. And then adding alkaline protease (papain: trypsin is 1: 1, and the enzyme activity is 40-50 ten thousand u/g) with the mass of 0.3-0.5% of the raw materials into the corresponding homogenate and the milk, carrying out enzymolysis for 1-2 h at the temperature of 45-55 ℃, and after enzymolysis, adjusting the temperature to 85-90 ℃ to inactivate the enzyme for 15-20 min. Filtering and centrifuging the enzymolysis liquid, and taking supernate to carry out ultrafiltration and centrifugation (the cut-off molecular weight is 30ku) on the micromolecule peptide. Specifically, the mass of the added alkaline protease is 0.4% of the mass of the raw materials, the enzymolysis temperature is 50 ℃, and the enzymolysis is carried out for 1.5 hours.
(3) And respectively adding 2-3 times of clear water into the washed sturgeon skin, soaking for 20-30 minutes, and homogenizing to obtain sturgeon skin homogenate. And then adding alkaline protease (papain: trypsin is 1: 1, and the enzyme activity is 40-50 ten thousand u/g) with the mass of 0.3-0.5% of the raw material into the corresponding homogenate, carrying out enzymolysis for 1-2 h at the temperature of 45-55 ℃, and after enzymolysis, adjusting the temperature to 85-90 ℃ to inactivate the enzyme for 15-20 min. Filtering and centrifuging the enzymolysis liquid, and taking supernate to carry out ultrafiltration and centrifugation (the cut-off molecular weight is 30ku) on the micromolecule peptide. Specifically, the mass of the added alkaline protease is 0.4% of the mass of the raw materials, the enzymolysis temperature is 50 ℃, and the enzymolysis is carried out for 1.5 hours.
(4) Adding alkaline protease (papain: trypsin is 1: 1, the enzyme activity is 40-50 ten thousand u/g) with the mass of 0.3-0.5% of the raw material into raw milk, carrying out enzymolysis for 1-2 h at the temperature of 45-55 ℃, and after enzymolysis, adjusting the temperature to 85-90 ℃ to inactivate the enzyme for 15-20 min. Filtering and centrifuging the enzymolysis liquid, and taking supernate to carry out ultrafiltration and centrifugation (the cut-off molecular weight is 30ku) on the micromolecule peptide. Specifically, the mass of the added alkaline protease is 0.5 percent of the mass of the raw materials, the enzymolysis temperature is 55 ℃, and the enzymolysis is carried out for 1 hour.
Examples 1 to 3
This example provides a series of sleep-aiding small peptide solid beverages, whose formulations are shown in table 1.
TABLE 1 sleep-aiding small-molecule peptide solid beverage of examples 1 to 3
Figure BDA0002722243680000061
Wherein, in examples 1-3 and comparative examples 1 and 3, the addition amount of the vitamin A is 504 mu g RAE/100 g; the addition amount of vitamin C is 200mg/100 g; the addition amount of vitamin D is 5 mug/100 g; the addition amount of vitamin E is 80mg alpha-TE/100 g; in the B vitamins, the addition amount of vitamin B1 is 10mg/100g, the addition amount of vitamin B2 is 12mg/100g, the addition amount of vitamin B6 is 8mg/100g, and the addition amount of vitamin B12 is 6mg/100 g.
Experimental example 1 sleep-aiding action of the sleep-aiding small-molecular-peptide solid beverages provided in examples 1 to 3 and comparative examples 1 to 3 on experimental mice
The method comprises the following steps: 190 healthy SPF-level Kunming mice (20 +/-2 g) are randomly selected, and are adaptively raised for one week and then randomly divided into 19 groups under the same condition, namely a blank control group (distilled water), a small-molecule peptide solid beverage low-dose group (1g/kg/d), a small-molecule peptide solid beverage medium-dose group (5g/kg/d) in example 1, a small-molecule peptide solid beverage high-dose group (15g/kg/d) in example 1, a small-molecule peptide solid beverage low-dose group (1g/kg/d) in example 2, a small-molecule peptide solid beverage medium-dose group (5g/kg/d) in example 2, a small-molecule peptide solid beverage high-dose group (15g/kg/d) in example 2, and a small-molecule peptide solid beverage low-dose group (1g/kg/d) in example 3, Example 3-Medium dose group of Small molecule peptide solid drink (5g/kg/d), example 3-high dose group of Small molecule peptide solid drink (15g/kg/d), comparative example 1-Low dose group of Small molecule peptide solid drink (1g/kg/d), comparative example 1-Medium dose group of Small molecule peptide solid drink (5g/kg/d), comparative example 1-high dose group of Small molecule peptide solid drink (15g/kg/d), comparative example 2-Low dose group of Small molecule peptide solid drink (1g/kg/d), comparative example 2-Medium dose group of Small molecule peptide solid drink (5g/kg/d), comparative example 2-high dose group of Small molecule peptide solid drink (15g/kg/d), comparative example 3-Low dose group of Small molecule peptide solid drink (1g/kg/d), Comparative example 3-small molecule peptide solid beverage medium dose group (5g/kg/d), comparative example 3-small molecule peptide solid beverage high dose group (15g/kg/d), each group is 10. The administration is carried out by means of intragastric administration, and the intragastric administration is carried out once at 20 points every night, and each group is continuously administered for 28 days by intragastric administration. Wherein the dosage group administration dosage (5g/kg/d) is converted by taking the limited administration amount of gamma-aminobutyric acid of 500mg per day of human body in the food safety law of the people's republic of China and the new resource food management method as a reference, and combining the conversion coefficient of the administration dosage of human and mouse according to the content of the gamma-aminobutyric acid in the small molecular solid beverage.
And sequentially carrying out a sleep-in test of the pentobarbital sodium mice with the subthreshold hypnotic dose, a sleep latency test of the pentobarbital sodium and a test of prolonging the sleep time of the pentobarbital sodium induced mice. The specific operation is as follows:
1. sub-threshold hypnotic dose pentobarbital sodium mouse sleep test
After administration of each dose group, the blank control group is administered with distilled water with the same volume, after 30min, each group is intraperitoneally injected with pentobarbital sodium at 150mg/kg, the influence of the sleep-aiding small molecular peptide on the sleep rate of mice injected with the subliminal pentobarbital sodium is observed by taking the disappearance of the righting reflex for more than 1min as a sleep-onset judgment standard. The results are shown in Table 1.
2. Pentobarbital sodium sleep latency test
After the administration of each dosage group, the blank control group is administered with distilled water with the same volume, after 30min, 300mg/kg of pentobarbital sodium is intraperitoneally injected into each group, the standard for judging sleep onset is that the time from the injection of the pentobarbital sodium to the sleep onset of the mice is more than 1min, and the sleep latency is determined. And observing the influence of the sleep-aiding small molecular peptide on the sleep latency of the sodium pentobarbital. The results are shown in Table 2.
3. Test for prolonging sleep time of pentobarbital sodium-induced mice
After the administration of each dose group, the blank control group is administered with distilled water with the same volume, after 30min, 300mg/kg of sodium pentobarbital is intraperitoneally injected into each group, the disappearance of righting reflex for more than 1min is taken as a sleep onset judgment standard, the recovery of the righting reflex indicates that the mouse is awake, and the time from the disappearance of the righting reflex to the recovery is the sleep time of the mouse. And observing the influence of the sleep-aiding small molecular peptide on the prolonging of the sleep time of the sodium pentobarbital. The results are shown in Table 3.
TABLE 1 influence of sleep-aiding small-molecule peptide solid beverage on the rate of falling asleep of mice injected with sodium pentobarbital
Figure BDA0002722243680000081
Note: compared with the blank control group, the composition of the composition,#P＜0.05，##P＜0.01。
TABLE 2 influence of sleep-aiding small-molecule peptide solid beverage on sleep latency of pentobarbital sodium in mice
Figure BDA0002722243680000082
Figure BDA0002722243680000091
Note: compared with the blank control group, the composition of the composition,#P＜0.05，##P＜0.01。
TABLE 3 influence of sleep-aiding small-molecule peptide solid beverage 1 on sleep time induced by pentobarbital sodium in mice
Figure BDA0002722243680000092
Note: compared with the blank control group, the composition of the composition,#P＜0.05，##P＜0.01。
as can be seen from tables 1, 2 and 3, the sleep-aiding small molecular peptide solid beverages with low, medium and high doses provided in the examples can significantly shorten the sleep latency time of the pentobarbital sodium in mice and prolong the sleep time of the pentobarbital sodium induced mice. Wherein, the sleep-aiding small molecular peptide solid beverage of the high-dosage group can obviously improve the sleep-onset rate of mice injected with the sodium pentobarbital. The results show that the sleep-aiding small molecular peptide solid beverage provided by the invention can synergistically play a sleep-aiding role with sodium pentobarbital on the basis of the hypnosis of the sodium pentobarbital. The sleep-aiding small molecular peptide solid beverage only selecting partial main materials or auxiliary materials (comparative examples 1-3) can shorten the sleep time of the pentobarbital sodium of the mouse and prolong the sleep time of the pentobarbital sodium induced mouse, but the whole sleep-aiding effect is inferior to that of the sleep-aiding small molecular peptide solid beverage provided by each embodiment.
According to the invention, the sleep-aiding small molecular peptide solid beverage obtained by compounding the main materials and the auxiliary materials has a remarkable sleep-aiding effect, and can greatly shorten the sleep latency time and prolong the sleep effective period time.
The above embodiments are preferred embodiments of the present invention, but the present invention is not limited to the above embodiments, and any other changes, modifications, substitutions, combinations, and simplifications which do not depart from the spirit and principle of the present invention should be construed as equivalents thereof, and all such changes, modifications, substitutions, combinations, and simplifications are intended to be included in the scope of the present invention.

Claims (10)

1. The sleep-aiding small molecular peptide solid beverage is characterized by comprising the following main materials and auxiliary materials in parts by weight:
Figure FDA0002722243670000011
auxiliary materials: 1-5 parts of gamma-aminobutyric acid;
1 to 5 parts of N-acetylneuraminic acid;
the addition amount of the compound vitamin is within the standard addition amount range of the national standard food additive.
2. The sleep-aiding small-molecule peptide solid beverage as claimed in claim 1, wherein the sturgeon skin oligopeptide is prepared by the following processes:
s11: soaking sturgeon skin, and homogenizing to obtain sturgeon skin homogenate;
s12: adding alkaline protease into the sturgeon skin homogenate, carrying out enzymolysis for 1-2 h at the temperature of 45-55 ℃, inactivating, filtering, and centrifuging to obtain the sturgeon skin oligopeptide.
3. The sleep-aiding small-molecule peptide solid beverage as claimed in claim 1, wherein the wheat oligopeptide is prepared by the following processes:
s21: soaking wheat, and homogenizing to obtain wheat homogenate;
s22: and adding alkaline protease into the wheat homogenate, carrying out enzymolysis for 1-2 h at the temperature of 45-55 ℃, inactivating, filtering, and centrifuging to obtain the wheat oligopeptide.
4. The sleep-aiding small-molecule peptide solid beverage as claimed in claim 1, wherein the pecan oligopeptide is prepared by the following steps:
s31: soaking hickory nuts and then homogenizing to obtain hickory nut homogenate;
s32: and adding alkaline protease into the hickory nut homogenate, carrying out enzymolysis for 1-2 h at the temperature of 45-55 ℃, inactivating, filtering, and centrifuging to obtain the hickory nut oligopeptide.
5. The sleep-aiding small-molecule peptide solid beverage as claimed in claim 1, wherein the milk oligopeptide is prepared by the following processes: adding alkaline protease into raw milk, carrying out enzymolysis for 1-2 h at the temperature of 45-55 ℃, inactivating, filtering, and centrifuging to obtain the milk oligopeptide.
6. The sleep-aiding small molecule peptide solid beverage as claimed in claim 1, wherein the compound vitamin comprises at least two of vitamin A, B family vitamin, vitamin C or vitamin E.
7. The sleep-aiding small-molecule peptide solid beverage as claimed in claim 6, wherein the addition amount of the vitamin A is 250-800 μ g RAE/100 g; the addition amount of the vitamin C is 30-500 mg/100 g; the addition amount of the vitamin D is 1.5-10 mu g/100 g; the addition amount of the vitamin E is 5.0-150 mg of alpha-TE/100 g; in the B vitamins, the addition amount of vitamin B1 is 0.5-20 mg/100g, the addition amount of vitamin B2 is 0.5-20 mg/100g, the addition amount of vitamin B6 is 0.5-10 mg/100g, and the addition amount of vitamin B12 is 1.0-10 mg/100 g.
8. The preparation method of the sleep-aiding small-molecule peptide solid beverage as claimed in any one of claims 1 to 7, which is characterized by comprising the following steps: and mixing sturgeon skin oligopeptide, wheat oligopeptide, pecan oligopeptide, cow milk oligopeptide, gamma-aminobutyric acid, N-acetylneuraminic acid and compound vitamin, and spray-drying to obtain the sleep-aiding small molecular peptide solid beverage.
9. Use of the sleep-aiding small-molecule peptide solid beverage of any one of claims 1 to 7 in preparation of sleep-aiding, nutritional restoration, organism immunity improvement or cosmetic drugs, health products or functional foods or preparations.
10. The use of claim 9, wherein the sleep-aiding small molecule peptide solid beverage is used for preparing a preparation for shortening the sleep latency period and prolonging the sleep valid period.
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