CN112042311A

CN112042311A - Method for improving germination rate of suaeda salsa seeds and planting method

Info

Publication number: CN112042311A
Application number: CN202010960512.8A
Authority: CN
Inventors: 张继伟; 赵昕; 陈国雄; 赵鹏善
Original assignee: Northwest Institute of Eco Environment and Resources of CAS
Current assignee: Northwest Institute of Eco Environment and Resources of CAS
Priority date: 2020-09-14
Filing date: 2020-09-14
Publication date: 2020-12-08

Abstract

The invention discloses a method for improving the germination rate of suaeda salsa seeds and a planting method, and relates to the technical field of planting. The method disclosed by the invention comprises the step of polishing the suaeda salsa seeds by using sand. The method reduces the obstruction of seed coat to water absorption by grinding the Salicornia Herbacea seeds, thereby relieving physical dormancy, promoting germination, increasing germination rate, and has positive effects on Salicornia Herbacea seedling survival, field planting flaking, wind proofing and sand fixing effects, etc.

Description

Method for improving germination rate of suaeda salsa seeds and planting method

Technical Field

The invention relates to the technical field of planting, in particular to a method for improving the germination rate of suaeda salsa seeds and a planting method.

Background

Sambucus, also known as Sago and Moyuzi, is an annual herb plant of Chenopodiaceae, and is widely distributed in desert regions of northwest, northwest and northeast provinces of China, and partial regions of Henan, Hebei, Shanxi and Tibet, and Mongolia, former Su Union Siberian and Central Asia regions. The agriophyllum squarrosum has the basic characteristics of cold resistance, barren resistance, wind erosion resistance, sand burying resistance and sand preference for soil. The plant is common in a floating sand dune or a semi-floating sand dune and a riverside sand bank, is a common sandy plant in the north of China, and is a pioneer plant for fixing the floating sand dune.

The awning can play a role in preventing wind and fixing sand; the seeds contain rich nutrient components and are good functional food; the plant can also be used as livestock feed. Therefore, the method has important significance for the research of the awning.

However, due to the characteristics of the wild plant seeds, the sprouting rate of the samphire seeds is low, and the samphire seeds are slow and irregular in sprouting. Can not germinate as fast as crops and grow regularly and consistently. Severely hampers the study work of the domestication of the agriophyllum squarrosum. Therefore, the method has the advantages of accelerating the germination rate of the suaeda salsa seeds, strengthening the survival of the seedlings, planting the seedlings into pieces and finally achieving the effects of wind prevention and sand fixation, and is significant and especially important.

In view of this, the invention is particularly proposed.

Disclosure of Invention

The invention aims to provide a method for improving the germination rate of suaeda salsa seeds and a planting method. The method reduces the obstruction of seed coat to water absorption by grinding the Salicornia Herbacea seeds, thereby relieving physical dormancy, promoting germination, increasing germination rate, and has positive effects on Salicornia Herbacea seedling survival, field planting flaking, wind proofing and sand fixing effects, etc.

The invention is realized by the following steps:

in one aspect, the invention provides a method for increasing the germination rate of suaeda salsa seeds, which comprises the following steps: and (4) polishing the sand seeds by using sand.

The inventor finds that the sand is used for mechanically grinding the suaeda salsa seeds, so that the obstruction of seed coats to water absorption is effectively reduced, the physical dormancy is relieved, the germination is promoted, and the germination rate is improved.

In an alternative embodiment, the grinding process comprises: and mixing the suaeda salsa seeds with sand, and placing the mixture on a grinding instrument for grinding.

In an alternative embodiment, the sand used has a particle size of less than or equal to 2mm when sanding is performed.

The inventor of the invention further finds that the sand with the proper grain size of less than or equal to 2mm is selected for grinding, which is more beneficial to improving the germination of the suaeda salsa seeds.

In an alternative embodiment, the sand used when sanding is performed has a particle size in the range of 1mm to 2 mm.

Further, the sand with the grain diameter of 1mm-2mm is selected for grinding, so that the germination rate of the suaeda salsa seeds is further improved.

In an alternative embodiment, the sanding is performed for a period of time no less than 30 seconds.

The inventors of the present invention found that sanding is also one of the factors affecting the germination rate of the seeds, and that the germination rate of the suaeda salsa seeds can be significantly increased with a sanding duration of at least 30 s.

In an alternative embodiment, the sanding is performed for a period of time in the range of 30s to 150 s.

The inventor of the invention further finds that the germination rate of the suaeda salsa seeds can be effectively improved by controlling the grinding time within the range of 30-150 s.

In an alternative embodiment, the weight ratio of the suaeda salsa seeds to the sand is 1: 1-1: 2, were mixed.

The use amount of the sand is too much or too little, which is not favorable for improving the germination rate, and researches show that the mass ratio of the sand seeds to the sand is controlled to be 1: 1-1: 2 is preferably in the range of 1: 1, it has promotion effect on improving the germination rate of the suaeda salsa seeds.

Placing the sand fleabane seeds in a container, and polishing the sand fleabane seeds by adopting a mode of oscillating the container;

preferably, the direction of oscillation is horizontal left and right oscillation.

Preferably, the oscillation frequency is 25-30 HZ;

preferably, the oscillation frequency is 30 HZ.

In another aspect, the present invention provides a method of growing a awning, comprising: before sowing, the sand fleabane seeds are subjected to a grinding treatment by using the method as described in any one of the above.

According to the planting method of the agriophyllum squarrosum provided by the invention, before sowing, the seeds are polished by using the polishing method, so that the germination rate of the seeds is improved, and the survival rate of the seedlings is further improved.

In an alternative embodiment, it comprises: and after polishing, sowing the suaeda salsa seeds.

In another aspect, the present invention provides a method for breeding suaeda salsa, comprising: before sowing, the Salicornia bigelovii seeds are treated using the method as described in any of the above.

According to the planting method of the agriophyllum squarrosum, provided by the invention, before sowing, the seeds are polished by using the polishing method, so that the germination rate of the seeds is improved, the survival rate of the seedlings is further improved, more agriophyllum squarrosum plants and progeny seeds can be obtained, and the breeding of the agriophyllum squarrosum is powerfully promoted.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings needed to be used in the embodiments will be briefly described below, it should be understood that the following drawings only illustrate some embodiments of the present invention and therefore should not be considered as limiting the scope, and for those skilled in the art, other related drawings can be obtained according to the drawings without inventive efforts.

FIG. 1 shows the measurement results of the germination rate and the imbibition rate of the sanded Salicornia herbacea seeds; a: germination rate results, B: and (4) obtaining the result of the swelling rate.

Fig. 2 is a photograph of the appearance of the seeds of the awning and different types of sand, scale bar: 1 cm.

FIG. 3 is a photograph of the growth of germinated Salicornia and transplanted Salicornia seedlings; a: germinating the Salicornia bigelovii Torr for 3 days; b: transplanting the Salicornia bigelovii seedling after 3 weeks.

Detailed Description

In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below. The examples, in which specific conditions are not specified, were conducted under conventional conditions or conditions recommended by the manufacturer. The reagents or instruments used are not indicated by the manufacturer, and are all conventional products available commercially.

The features and properties of the present invention are described in further detail below with reference to examples.

Example 1

The method for improving the germination rate of the suaeda salsa seeds, provided by the embodiment, comprises the following steps:

0.25g of Salicornia bigelovii seeds are weighed into a 2ml microfuge tube. And then filling sand (named as medium sand) with the same mass and the particle size of 1MM-2MM into the micro centrifugal tube, putting the micro centrifugal tube into a grinding instrument (Retsch MM400), starting the grinding instrument, horizontally oscillating the centrifugal tube left and right, and setting the grinding time to be 30s and the frequency to be 30 HZ.

Example 2

The method for improving the germination rate of the suaeda salsa seeds provided by the embodiment is basically the same as the step of the embodiment 1, except that the grinding time is 60 s.

Example 3

The method for improving the germination rate of the suaeda salsa seeds provided by the embodiment is basically the same as the step of the embodiment 1, except that the grinding time is 90 s.

Example 4

The method for improving the germination rate of the suaeda salsa seeds provided by the embodiment is basically the same as the step of the embodiment 1, except that the grinding time is 120 s.

Example 5

The method for improving the germination rate of the suaeda salsa seeds provided by the embodiment is basically the same as the step of the embodiment 1, except that the grinding time is 150 s.

Experimental example 1

Germination rate detection

The agriophyllum squarrosum seeds treated by the methods of examples 1-5 were used as experimental groups and the agriophyllum squarrosum seeds without sanding were used as control groups. Adding 2ml of sterile water into a 9cm plate with two layers of filter paper in advance, placing 50 seeds on the filter paper for germination experiments, and detecting the germination rate of each group, wherein each group is subjected to 5 times of repetition, and each group repeatedly uses 100 seeds. The results are shown in Table 1 below and A in FIG. 1.

TABLE 1 Germination Rate of Salicornia Bigelivii Torr seeds with the same particle size but different polishing time

Grouping	Sand particle size	Length of time of polishing	Germination rate
				Control group	-	0s	16.8％
Example 1	1mm-2mm	30s	91.7％
				Example 2	1mm-2mm	60s	97.6％
Example 3	1mm-2mm	90s	93.2％
				Example 4	1mm-2mm	120s	95.2％
Example 5	1mm-2mm	150s	97.2％

The results in Table 1 and FIG. 1 show that the germination rate of the Salicornia herbacea seeds is greatly improved by the methods of examples 1-5, and the germination rate can be improved to 97.6% from 16.8% of the control group by grinding for 60 s.

Experimental example 2

The water absorption (i.e., swelling ratio) of each group was measured by conducting a water absorption test using the agriophyllum squarrosum seeds treated by the methods of examples 1 and 5 as an experimental group and using agriophyllum squarrosum seeds not subjected to grinding as a control group.

The water absorption experiment method comprises the following steps: soaking the sand-polished salicornia bigelovii seeds in sterile water for 2 hours, and weighing the weight of the seeds before and after soaking by using an electronic balance, wherein the seed imbibition rate is (the weight of the seeds after soaking-the weight of the seeds before soaking)/the weight of the seeds before soaking;

as can be seen from B in FIG. 1, the water absorption rate of the seeds within 2h can be significantly increased by grinding the Salicornia bigelovii seeds for 30s and 150s, and the seed swelling rate is respectively increased from 6.04% to 54.38% and 55.97% of the control group. The method shows that the sand is used for polishing the suaeda salsa seeds, so that the barrier of the seed coats to water absorption can be effectively reduced, the physical dormancy is relieved, and the germination is promoted.

Experimental example 3

Sand of different particle size was used: fine sand (particle size less than 1mm) and coarse sand (particle size 2mm < particle size 5mm) (see fig. 2), the suaeda salsa seeds were polished for 30s according to the method of example 1, the germination rate after fine sand polishing was 2.95%, and the germination rate after coarse sand polishing was 84.89%.

The results show that fine sand sanding has substantially no effect on the germination of the agriophyllum squarrosum seeds, while medium sand and coarse sand sanding can significantly improve the germination rate of the agriophyllum squarrosum. In addition, through observing the seeds after polishing, the damage of the coarse sand polishing to the seeds is found to be serious. Most suitably, the sand is used to grind the Salicornia bigelovii seeds to promote germination.

Experimental example 4

Carefully transplanting the germinated buds in the experimental example 1 to a soil culture medium, and obtaining the robust and tidy samphire seedlings (see figure 3) after 3 weeks in indoor common illumination, wherein the transplanting survival rate can reach more than 95%.

The seedling of the agriophyllum squarrosum cultivated by the method can completely meet various indoor research needs. In addition, for the agriophyllum squarrosum seeds with limited seed sources, the method can also effectively avoid the loss of important resources. Before the dormant agriophyllum squarrosum variety is cultured, the transplanted seedling can be transferred to a test field, which is beneficial to promoting the field cultivation and domestication of the agriophyllum squarrosum.

The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A method for improving the germination rate of suaeda salsa seeds is characterized by comprising the following steps: and (4) polishing the sand seeds by using sand.

2. The method of claim 1, wherein the grinding process comprises: and mixing the suaeda salsa seeds with sand, and placing the mixture on a grinding instrument for grinding.

3. The method of claim 2, wherein the sanding is performed with a sand size of 2mm or less.

4. A method according to claim 3, wherein the sand used in the sanding is of a size in the range 1mm to 2 mm.

5. The method of claim 2, wherein the sanding is performed for a period of time not less than 30 seconds;

preferably, the sanding is performed for a period of time in the range of 30s to 150 s.

6. The method according to any one of claims 2 to 5, wherein the weight ratio of the Salicornia bigelovii seeds to the sand is 1: 1-1: 2, mixing;

preferably, the mass ratio is 1: 1.

7. the method of claim 2, wherein the Salicornia Herbacea is mixed with sand, placed in a container, and the Salicornia Herbacea is ground by shaking the container;

preferably, the oscillation direction is horizontal oscillation left and right;

preferably, the oscillation frequency is 25-30 HZ;

preferably, the oscillation frequency is 30 HZ.

8. A method of growing a awning, comprising: before sowing, the seeds of the agriophyllum squarrosum are subjected to a sanding treatment using the method according to any one of claims 1 to 7.

9. The method according to claim 8, characterized in that it comprises: and after polishing, sowing the suaeda salsa seeds.

10. A method for breeding agriophyllum squarrosum, which is characterized by comprising the following steps: before sowing, the treatment of the Salicornia bigelovii seeds with the method according to any of claims 1 to 7.