CN112023701B - Heating type sample impurity-removing protein filtering device - Google Patents
Heating type sample impurity-removing protein filtering device Download PDFInfo
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- CN112023701B CN112023701B CN202011213031.7A CN202011213031A CN112023701B CN 112023701 B CN112023701 B CN 112023701B CN 202011213031 A CN202011213031 A CN 202011213031A CN 112023701 B CN112023701 B CN 112023701B
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- G01N1/34—Purifying; Cleaning
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
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Abstract
The invention relates to a heating type sample impurity protein removing and filtering device which comprises a tray module, a film pressing module and a heating module, wherein the film pressing module is arranged above the tray module; the tray module comprises a reagent strip tray and one or more reagent strips detachably fixed on the reagent strip tray, and the reagent strips are provided with a protein removing device; the film pressing module comprises a pressing block and a telescopic device for controlling the pressing block to move up and down, and the pressing block can apply downward pressure to the protein removing device; the heating module comprises a heating body and a heating sheet, the heating sheet is arranged on the side face of the heating body, one or more heating holes are formed in the heating body, and the protein removing device can be inserted into the heating holes. The invention has the beneficial effects that: the full-automatic operation equipment is adopted to replace manual work, the operation can be performed in a row, and a plurality of sample liquids are processed at one time; the heating temperature is controllable, and the requirements of different experimental samples can be met; meanwhile, the film pressing process is also controllable, and different film pressing filtering speeds can be selected according to experiment requirements and sample types.
Description
Technical Field
The invention belongs to the field of biochemical immunoassay equipment, and particularly relates to a heating type sample impurity protein removing and filtering device.
Background
Methods for detecting carbohydrate markers exist in both the biochemical and microbiological fields, where removal of contaminating proteins is a necessary and routine step, and removal of proteins can be accomplished by a very wide variety of methods in a laboratory setting, including adjustment of isoelectric point, adjustment of pH, addition of organic solvents or surfactants such as alcohols, acetone, etc., high temperature heating, addition of adsorbents, passage through ion exchange columns, etc. These methods are relatively common in the laboratory, but are not easy if they are simple and easy to use as products. Each method has its own limitations and some methods are not suitable for use in products. Such as ion exchange, using an ion exchange column. The ion exchange column is prepared by mixing cation and anion exchange resins in a certain proportion and filling the mixture in the same exchange device, and exchanging and removing ions in fluid. The equipment can be only equipped in a professional laboratory, and the popularization is not strong. The remaining methods are also subject to trade-offs depending on the nature of the substance to be detected. For saccharides with good thermal stability, a very simple method for removing proteins is to heat the sample at high temperature to denature the proteins. Finally, the denatured protein is filtered out to obtain a clear marker to be detected. Two common methods are used for filtering out denatured proteins, the first is to precipitate by centrifugation; the second is by way of filtration. Both methods need to be realized by means of complex equipment, and balance adjustment and centrifugal force setting are realized in centrifugation; the filtration mode is to realize the installation of the filter membrane and the pressure supply. Cannot be simply and rapidly completed.
Patent 2016100165094 discloses a device for removing foreign proteins, but this patent requires manual operation, which is inefficient, and only one operation can be done at a time; and different samples have requirements on the filtering speed and the filtering pressure of the filtering device, the filtering membrane is easy to break when the filtering speed is too fast, and the filtering time is too slow and too long.
Disclosure of Invention
In order to solve the technical problem, the invention provides a heating type sample deproteinization filtering device.
The technical scheme adopted by the invention is as follows: the heating type sample impurity protein removing and filtering device comprises a tray module, a film pressing module and a heating module, wherein the film pressing module is arranged above the tray module, and the heating module is arranged below the tray module; the tray module, the film pressing module and the heating module are all arranged on the mounting frame;
the tray module comprises a reagent strip tray and one or more reagent strips detachably fixed on the reagent strip tray, and the reagent strips are provided with a protein removing device;
the film pressing module comprises a pressing block and a telescopic device for controlling the pressing block to move up and down, and the pressing block can apply downward pressure to the protein removing device;
the heating module comprises a heating body and a heating sheet, the heating sheet is arranged on the side face of the heating body, one or more heating holes are formed in the heating body, and the protein removing device can be inserted into the heating holes.
Preferably, horizontal moving devices are arranged at two ends of the tray module.
Preferably, the horizontal migration device includes horizontal guide, horizontal slider, horizontal gear and horizontal migration motor, and horizontal slider cooperatees with horizontal guide, and horizontal slider bottom is equipped with the rack, and the symmetry is equipped with horizontal guide on the mounting bracket, and reagent strip tray both ends fixedly connected with horizontal slider, horizontal gear set up in mounting bracket one side, can with the rack cooperation on the horizontal slider, horizontal gear is connected with the horizontal migration motor.
Preferably, two ends of the heating module are provided with lifting devices;
preferably, the outer side of the heating module is also provided with a heat-insulating cover, the heat-insulating cover is arranged on the outer side of the heating sheet, or the heat-insulating cover and the heating sheet are respectively arranged on two sides of the heating body;
preferably, a temperature probe is arranged in the heating body.
Preferably, the lifting device comprises a vertical guide rail, vertical sliding blocks, vertical gears and a vertical moving motor, the vertical sliding blocks are matched with the vertical guide rail, racks are arranged on the vertical sliding blocks, the vertical guide rail is symmetrically arranged on the mounting rack, the two ends of the heating body are fixedly connected with the vertical sliding blocks, the vertical gears are arranged on one side of the mounting rack and can be matched with the racks on the vertical sliding blocks, and the vertical gears are connected with the vertical moving motor.
Preferably, the lifting device comprises guide grooves, guide wheels, a rotating shaft, an inner connecting rod and a lifting motor mechanism, the guide grooves are symmetrically arranged on the mounting rack, the guide wheels are arranged at two ends of the heating body and embedded in the guide grooves, the rotating shaft is rotatably arranged on the mounting rack and connected with the heating body through the inner connecting rod, and the rotating shaft is connected with the lifting motor mechanism;
preferably, the inner connecting rod comprises a first connecting rod and a second connecting rod which are hinged, the other end of the first connecting rod is connected with the rotating shaft, and the other end of the second connecting rod is hinged with the heating body.
Preferably, the lifting motor mechanism comprises a driving motor, a short swing arm, a long swing arm and a motor connecting rod, the short swing arm and the long swing arm are respectively hinged to two ends of the motor connecting rod, the other end of the short swing arm is connected with the driving motor, and the other end of the long swing arm is connected with the rotating shaft.
Preferably, the telescopic device is fixed on the film pressing frame, the film pressing frame comprises a film pressing top plate and two film pressing side plates arranged at two ends of the film pressing top plate, the fixed end of the telescopic device is fixedly arranged on the film pressing top plate, and the movable end of the telescopic device is connected with the pressing block;
preferably, the telescoping device includes step motor, synchronous wheelset and lead screw, and the briquetting is connected to the lead screw lower extreme, and the lead screw other end passes the press mold roof and connects synchronous wheelset, and step motor connects synchronous wheelset.
Preferably, be equipped with the press mold guide rail on the press mold frame, the press mold guide rail sets up on the press mold curb plate, perhaps sets up on the press mold backplate between two press mold curb plates, is equipped with the briquetting guide way on the briquetting, and briquetting guide way position matches with the press mold guide rail.
Preferably, still include position sensor and contact, position sensor is fixed to be set up on the die pressing frame, and the contact setting is on the briquetting, and the position sensor can be blocked to the contact.
The invention has the advantages and positive effects that: according to the scheme, full-automatic operation equipment is adopted to replace manual work, the operation can be performed in a row, and a plurality of sample liquids are processed at one time; the heating temperature is controllable, the requirements of different experimental samples can be met, and manual intervention is not needed; meanwhile, the film pressing process is also controllable, and different film pressing filtering speeds can be selected according to experiment requirements and sample types.
Drawings
FIG. 1 is a schematic structural view of a heating state according to an embodiment of the present invention;
FIG. 2 is a schematic view of a pressed film structure according to an embodiment of the present invention;
FIG. 3 is a partial block diagram of one embodiment of the present invention;
FIG. 4 is a schematic structural diagram of a protein removing device according to an embodiment of the present invention;
FIG. 5 is a schematic diagram of a heating module according to an embodiment of the present invention;
FIG. 6 is a schematic diagram showing a structural comparison of two positions of a heating module according to an embodiment of the present invention;
FIG. 7 is a schematic view of a heater configuration according to an embodiment of the present invention;
FIG. 8 is a schematic cross-sectional view of a heating body according to an embodiment of the present invention;
FIG. 9 is a side view of a tray module and heating module according to one embodiment of the present invention;
FIG. 10 is a schematic view of a lamination module according to an embodiment of the present invention;
fig. 11 is a schematic view of a lamination module according to an embodiment of the present invention.
In the figure: 1. a side vertical plate; 2. a film pressing module; 211. pressing a film top plate; 212. pressing a film side plate; 22. briquetting; 231. a stepping motor; 232. a primary wheel; 233. a synchronous belt; 234. a sub-wheel; 235. a tension wheel; 24. a lead screw; 25. a film pressing guide rail; 261. a positioning sensor; 262. a contact piece; 3. a tray module; 31. a reagent strip tray; 32. a horizontal guide rail; 33. a horizontal slider; 34. a horizontal gear; 35. a horizontal movement motor; 4. a heating module; 4A, an initial position; 4B, lifting the heating position; 41. a heating body; 42. a heat generating sheet; 43. a heat-preserving cover; 44. heating the hole; 45. a temperature probe; 461. a guide groove; 462. a guide wheel; 471. a rotating shaft; 472. an inner link; 481. a drive motor; 482. a short swing arm; 483. a motor connecting rod; 484. a long swing arm; 5. a protein removing device; 51. a sample bin; 52. filtering the membrane; 53. and a filter element barrel.
Detailed Description
The invention is described below with reference to the accompanying drawings.
As shown in fig. 1-2, the heating type sample removing and impurity protein filtering device comprises a tray module 3, a film pressing module 2 arranged above the tray module 3 and a heating module 4 arranged below the tray module 3; the tray module 3 is internally provided with a protein removing device 5 for containing sample liquid to be filtered, and one or more than one protein removing device can be arranged according to the requirement; the heating module 4 is used for providing a heating environment for the protein removing device 5 and providing a proper heating condition according to requirements; the film pressing module 2 moves downwards to press the protein removing device 5, and the filtering process is completed. Tray module 3, press mold module 2 and heating module 4 all set up on the mounting bracket, and the mounting bracket can be on two relative side riser 1.
The protein removing device 5 used in the present invention has the same structure as that disclosed in patent 2016100165094, and as shown in fig. 4, includes a sample bin 51 for carrying a sample liquid to be filtered, a filter cartridge 53 capable of being inserted into the sample bin 51 to store the filtered sample liquid, a hole is provided at the lower end of the filter cartridge 53, a filter membrane 52 is provided at the bottom and the outer wall of the filter cartridge 53 for filtering the sample liquid, the upper end of the filter cartridge 53 is exposed outside the range of the sample bin 51, when the filter cartridge 53 is pressed down, the sample liquid to be filtered in the sample bin 51 is pressed through the filter membrane 52 and enters the filter cartridge 53, and as the filter cartridge 53 is continuously moved down under the pressure, more sample liquid enters the filter cartridge 53 through the filter membrane 52, thereby achieving the purpose of removing the protein. The edge of the sample chamber 51 extends outwards and can be clamped in a hole-shaped structure matched with the shape of the sample chamber 51.
As shown in FIG. 3 and FIG. 9, the tray module 3 comprises a reagent strip tray 31 and reagent strips detachably fixed on the reagent strip tray 31, the reagent strips comprise one or more reagent strips, the reagent strips are provided with a protein removing device 5, the reagent strip tray 31 is provided with slots, the end parts of the reagent strips are provided with plug-in structures, the reagent strips can be inserted into the slots through the plug-in structures, so that the reagent strips are fixed on the reagent strip tray 31, each reagent strip is provided with a sample bin 51 structure, and when the reagent strip tray is used, the filter cartridge 53 can be manually inserted into the sample bin 51. In some embodiments of the present invention, 1-12 reagent strips can be arranged in a row in the reagent strip tray 31, and the process of removing the impure protein can process 1-12 sample solutions at the same time.
In some embodiments of the present invention, in order to reduce the size of the whole apparatus and facilitate the use and installation, as shown in fig. 3 and fig. 9, the tray module 3 is configured to be horizontally movable, and when the tray is located at the front end or the rear end, the tray cooperates with the heating module 4 to heat the sample liquid, and when the sample liquid is heated, the tray is separated from the heating module 4 and moved to the other end to perform the filtering process under the action of the squeeze film module 2. Horizontal moving means may be provided at both ends of the tray module 3. The horizontal moving device comprises a horizontal guide rail 32, a horizontal sliding block 33, a horizontal gear 34 and a horizontal moving motor 35, wherein the horizontal sliding block 33 is matched with the horizontal guide rail 32, and the horizontal sliding block 33 can reciprocate back and forth in the range of the horizontal guide rail 32; the horizontal guide rails 32 are symmetrically arranged on the inner sides of the two side vertical plates 1, the two ends of the reagent strip tray 31 are fixedly connected with horizontal sliding blocks 33, and reagent strips can horizontally move forwards and backwards along the horizontal guide rails 32 under the action of the horizontal sliding blocks 33 and the horizontal guide rails 32; the horizontal sliding block 33 or the two horizontal sliding blocks 33 on one side are provided with racks at the bottom, the horizontal gear 34 is arranged on the inner side of the side vertical plate 1 on one side and can be meshed with the racks on the horizontal sliding block 33, and the horizontal gear 34 is connected with a horizontal moving motor 35; when the horizontal moving motor 35 rotates, the horizontal gear 34 is driven to rotate, so as to drive the horizontal slider 33 to move back and forth on the horizontal guide rail 32, and the tray module 3 moves back and forth horizontally.
As shown in fig. 7, the heating module 4 includes a heating body 41 and a heating sheet 42, and can be used for heating the protein removing device 5 loaded on the reagent strip tray 31, one or more heating holes 44 are provided in the heating body 41, the number of the heating holes is consistent with the number of the reagent strip mounting positions, and the positions of the heating holes are matched, and the protein removing device 5 can be inserted into the heating holes 44; the heating sheet 42 is arranged on the side surface of the heating body 41 and used for providing heat energy, the heat-insulating cover 43 is also arranged on the outer side of the heating module 4, and the heat-insulating cover 43 can be arranged on the outer side of the heating sheet and used for isolating a heat source, so that the waste of the heat source is avoided, meanwhile, the heat source diffusion is also avoided to influence the working environment of the whole equipment, and the temperature control is convenient; or in order to save heat energy, only one side is provided with the heating sheet, the heat-insulating cover 43 and the heating sheet are respectively arranged at two sides of the heating body 41, so that the heating body 41 in the middle is guaranteed to be heated in a controlled manner, and the structure can save heat energy, reduce the volume of the device and facilitate the installation of equipment; as shown in fig. 8, a temperature probe 45 is provided in the heating body 41 for feeding back the heating temperature of the heating body 41.
In some embodiments of the present invention, the heating module 4 may be fixedly disposed below the tray module 3, which is not movable, and covers the sample chamber 51 of the deproteinizing device 5; in some embodiments of the present invention, the heating module 4 may be configured to move up and down, when heating is needed, the heating module 4 rises to wrap the sample bin 51, and after heating is completed, the heating module 4 descends to separate from the sample bin 51 of the protein removing device 5, so as to avoid influence on the protein removing process due to overheating, and also avoid influence on the back-and-forth movement behavior of the tray module 3, so that lifting devices are disposed at two ends of the heating module 4.
In some embodiments of the present invention, the lifting device has a structure similar to that of the horizontal moving device of the tray module 3, and includes a vertical guide rail, a vertical slider, a vertical gear and a vertical moving motor, the vertical slider is matched with the vertical guide rail, so that the vertical slider can slide up and down on the vertical guide rail, the vertical guide rail is symmetrically arranged inside the two side vertical plates 1, and the two ends of the heating body 41 are fixedly connected with the vertical slider, so that the heating body 41 can move up and down along the vertical guide rail; a rack is arranged on the vertical sliding block on one side or two sides, a vertical gear is arranged on the inner side of the side vertical plate 1 on one side and can be meshed with the rack on the vertical sliding block, and the vertical gear is connected with a vertical moving motor; when the vertical motor rotates, the vertical gear can be driven to rotate, so that the vertical sliding block is driven to move up and down on the vertical guide rail, and the heating body 41 can move up and down.
In order to further reduce the overall size of the device, in some embodiments of the present invention, the heating module 4 may be designed to turn within a certain angle range instead of a straight up-down lifting process. As shown in fig. 5, the lifting device includes a guide slot 461, a guide wheel 462, a rotating shaft 471, an inner connecting rod 472 and a lifting motor mechanism, the two side vertical plates 1 are symmetrically provided with the guide slot 461, two ends of the heating body 41 are provided with the guide wheels 462, the guide wheels 462 are embedded in the guide slot 461, so that the heating body 41 can be turned over and moved within the range of the guide slot 461; in order to maintain the stable movement of the heating body 41, two guide wheels 462 may be provided at each side, and the guide groove 461 may be provided in an "L" shape, and the two guide wheels 462 may move in the "L" shaped guide groove 461; to facilitate the installation of the guide wheel 462, the top end of the guide slot 461 can be extended outward to the edge of the side standing plate 1. The pivot 471 is rotatable to be set up between two side riser 1, parallel with heating body 41, the pivot 471 passes through the inner connecting rod 472 with heating body 41 and is connected, the inner connecting rod 472 includes first connecting rod of articulated and second connecting rod, pivot 471 is connected to the first connecting rod other end, the other end of second connecting rod is articulated with heating body 41, pivot 471 one section is connected with elevator motor mechanism, elevator motor mechanism drives the pivot 471 and rotates, change heating body 41 position under the effect of inner connecting rod 472, make heating body 41 realize the upset action under the effect of leading wheel 462 and guide way 461, as shown in fig. 6, reciprocate for tray module 3.
In some embodiments of the present invention, the lift motor may be a motor structure directly; in some embodiments of the present invention, in order to reduce the installation occupation range, the motor may be installed inside the side vertical plate 1, and then connected to the rotating shaft 471 through a link device. The lifting motor mechanism comprises a driving motor 481, a short swing arm 482, a long swing arm 484 and a motor connecting rod 483, wherein the short swing arm 482 and the long swing arm 484 are respectively hinged at two ends of the motor connecting rod 483, the other end of the short swing arm 482 is connected with the driving motor 481, the other end of the long swing arm 484 is connected with a rotating shaft 471, and the driving motor 481 drives the rotating shaft 471 to rotate through the connecting rod device.
Film pressing module 2 includes briquetting 22 and the telescoping device that controls briquetting 22 and reciprocate, and the telescoping device stiff end is fixed on the roof of connecting at two side riser 1 tops, removes the end and connects briquetting 22, and under the drive of telescoping device, briquetting 22 can be to removing albumen device 5 and exert decurrent pressure. In some embodiments of the present invention, the telescopic device is fixed on the film pressing frame, the film pressing frame includes a film pressing top plate 211 and two film pressing side plates 212 arranged at two ends of the film pressing top plate 211, the rear sides of the two film pressing sides are further connected with a film pressing back plate, the fixed end of the telescopic device is fixed on the film pressing top plate 211, and the moving end is connected with the pressing block 22 through a bearing seat; in some embodiments of the present invention, the telescopic device may be a cylinder or a lead screw 24 structure connected with a motor; in other embodiments of the present invention, as shown in fig. 10-11, the telescoping device includes a stepping motor 231, a synchronous wheel set and a lead screw 24, the lower end of the lead screw 24 is connected to the pressing block 22, and the upper end of the lead screw 24 passes through the film pressing top plate 211 to be connected to the synchronous wheel set; the synchronous pulley set comprises a sub-pulley 234, a main pulley 232 and a synchronous belt 233 which is meshed with the sub-pulley 234 and the main pulley 232, the corresponding synchronous belt 233 further penetrates through a tension pulley 235, the screw rod 24 and the stepping motor 231 are respectively connected with the sub-pulley 234 and the main pulley 232 in the synchronous pulley set, the stepping motor 231 can be arranged at the bottom of the film pressing top plate 211 through the arrangement of the synchronous pulley, and installation space is saved.
In order to ensure that the pressing block 22 moves vertically, a film pressing guide rail 25 can be arranged on the film pressing frame, the film pressing guide rail 25 is arranged on the film pressing side plates 212 on one side or two sides or on the film pressing back plate, a pressing block 22 guide groove 461 is arranged on the pressing block 22, and the position of the pressing block 22 guide groove 461 is matched with the film pressing guide rail 25; the stable movement of the pressing block 22 is limited by the cooperation between the guide groove 461 of the pressing block 22 and the film pressing guide 25. In order to prompt the position of the pressing block 22, in some embodiments of the present invention, the pressing block 22 further includes a positioning sensor 261 and a contact 262, the positioning sensor 261 is a photoelectric sensor, the positioning sensor 261 is fixedly disposed on the pressing frame, the contact 262 is disposed on the pressing block 22, the contact 262 can block the positioning sensor 261, the pressing block 22 rises to the highest point, the contact 262 blocks the signal of the positioning sensor 261, and when the pressing block 22 does not work, the pressing block is kept at the highest point, so as to avoid affecting the operation of other modules.
The device is also provided with a control circuit which is connected with the motor structures in the modules, and when the device is also provided with a positioning sensor 261 or a temperature probe 45, the device is also connected with the control circuit.
Example (b):
the heating type sample impurity protein removing and filtering device comprises a tray module 3, a film pressing module 2 arranged above the tray module 3, a heating module 4 arranged below the tray module 3 and a control circuit; the tray module 3, the film pressing module 2 and the heating module 4 are all arranged on the two opposite side vertical plates 1.
The tray module 3 comprises a reagent strip tray 31 and one or more reagent strips detachably fixed on the reagent strip tray 31, and the reagent strips are provided with a protein removing device 5; the protein removing device 5 comprises a sample bin 51 for bearing sample liquid to be filtered and a filter element barrel 53 which can be inserted into the sample bin 51 to store the filtered sample liquid, the sample bin 51 is arranged on the reagent strip, the lower end of the filter element barrel 53 is provided with a hole, and the bottom and the outer wall of the filter element barrel 53 are wrapped with filter membranes 52; tray module 3 both ends are equipped with horizontal migration device, horizontal migration device includes horizontal guide 32, horizontal slider 33, horizontal gear 34 and horizontal migration motor 35, horizontal slider 33 cooperatees with horizontal guide 32, horizontal slider 33 bottom is equipped with the rack, the symmetry is equipped with horizontal guide 32 on two side riser 1, reagent strip tray 31 both ends fixedly connected with horizontal slider 33, horizontal gear 34 sets up 1 inboards on the side riser of one side, can with the rack cooperation on the horizontal slider 33, horizontal gear 34 is connected with horizontal migration motor 35.
The two ends of the moving range of the reagent strip tray 31 are respectively a heating position and a film pressing position, the protein removing device 5 is arranged on the reagent strip, the reagent strip is inserted into the reagent strip tray 31 in the experiment preparation process, the sample liquid to be processed is manually added into the sample bin 51, and the filter core barrel 53 is arranged; when reagent strip tray 31 is in the heating position, heating module 4 rises, and the parcel removes albumen device 5 and heats, and the back that finishes heats, reagent strip tray 31 removes the press mold position, and press mold module 2 briquetting 22 descends and realizes filtration process.
The heating module 4 comprises a heating body 41, a heating sheet 42 and a heat-insulating cover 43, wherein a temperature probe 45 is arranged in the heating body 41, the heating sheet 42 and the heat-insulating cover 43 are respectively arranged on two sides of the heating body 41, one or more heating holes 44 are arranged in the heating body 41, and the bottom of the protein removing device 5 can be inserted into the heating holes 44. Two ends of the heating module 4 are provided with lifting devices; the lifting device comprises a guide groove 461, guide wheels 462, a rotating shaft 471, an inner connecting rod 472 and a lifting motor mechanism, the guide grooves 461 are symmetrically arranged on the two side vertical plates 1, the guide wheels 462 are arranged at the two ends of the heating body 41, the guide wheels 462 are embedded in the guide grooves 461, the rotating shaft 471 is rotatably arranged between the two side vertical plates 1, the rotating shaft 471 is connected with the heating body 41 through the inner connecting rod 472, the inner connecting rod 472 comprises a first connecting rod and a second connecting rod which are hinged, the other end of the first connecting rod is connected with the rotating shaft 471, the other end of the second connecting rod is hinged with the heating body 41, and the rotating shaft 471 is; the lifting motor mechanism comprises a driving motor 481, a short swing arm 482, a long swing arm 484 and a motor connecting rod 483, wherein the short swing arm 482 and the long swing arm 484 are respectively hinged at two ends of the motor connecting rod 483, the other end of the short swing arm 482 is connected with the driving motor 481, the other end of the long swing arm 484 is connected with a rotating shaft 471, and the driving motor 481 is arranged on the inner side of the side vertical plate 1.
When the heating device is used, the heating sheet 42 is heated by the control circuit to drive the heating body 41 to be heated, and after the preset temperature is reached, the heating body 41 is lifted to a heating position to heat the protein removing device 5; specifically, the heating body 41 ascends, namely, the motor rotates the rotating shaft 471 through the short swing arm 482, the connecting rod and the long swing arm 484 in sequence, and the rotating shaft 471 drives the heating body 41 through the inner connecting rod 472 to move upwards in the range of the guide groove 461; the heating body 41 is turned over at a certain angle, and the initial position 4A of the heating body 41 is in a half-flat lying state, so that enough space is ensured for operating the reagent strips.
The film pressing module 2 comprises a pressing block 22 and a telescopic device for controlling the pressing block 22 to move up and down, the pressing block 22 can apply downward pressure to the protein removing device 5, and the pressing block 22 can cover all the protein removing devices 5 on the tray module 3; the telescopic device is fixed on the film pressing frame, the film pressing frame comprises a film pressing top plate 211 and two film pressing side plates 212 arranged at two ends of the film pressing top plate 211, film pressing back plates are further arranged at the rear sides of the two film pressing side plates 212, the fixed end of the telescopic device is fixedly arranged on the film pressing top plate 211, and the moving end of the telescopic device is connected with the pressing block 22; the telescopic device comprises a stepping motor 231, a synchronous wheel set and a lead screw 24, the lower end of the lead screw 24 is connected with the pressing block 22, the other end of the lead screw 24 penetrates through the film pressing top plate 211 to be connected with the synchronous wheel set, and the stepping motor 231 is connected with the synchronous wheel set; the synchronous wheel set comprises a sub wheel 234, a main wheel 232 and a synchronous belt 233 meshed with the sub wheel 234 and the main wheel 232, the corresponding synchronous belt 233 further penetrates through a tension wheel 235, the screw rod 24 and the stepping motor 231 are respectively connected with the sub wheel 234 and the main wheel 232 in the synchronous wheel set, and the stepping motor 231 can be arranged at the bottom of the film pressing top plate 211 through the arrangement of the synchronous wheel, so that the installation space is saved; film pressing guide rails 25 are arranged on two sides of the film pressing back plate, a guide groove 461 is arranged on the pressing block 22, and the position of the guide groove 461 of the pressing block 22 is matched with the film pressing guide rails 25; still include location sensor 261 and contact 262, location sensor 261 is fixed to be set up on the press mold backplate, and contact 262 sets up on briquetting 22, and location sensor 261 can be blocked to contact 262.
When the pressing block 22 is used, the stepping motor 231 drives the synchronous belt 233, the synchronous belt 233 drives the lead screw 24, and the nut of the lead screw 24 moves upwards or downwards along with the lead screw 24 to drive the pressing block 22 to move up and down; the pressing block 22 moves up and down under the limiting action of the guide rail, so that the two ends of the pressing block 22 stably and synchronously operate when moving up and down, the deflection is avoided, and stable pressure is applied to the protein removing device 5 at the lower end; the pressing block 22 has two positions, namely an initial position 4A and a pressing position, the initial position is at the initial position 4A, the photoelectric sensor and the contact sheet 262 send position information to the control circuit to control the motor to stop, the pressing operation is gradually pressed downwards by the control circuit according to the program setting, the downward movement is stopped after the maximum value of the pressing position is reached, and the upward and downward movement of the pressing block 22 realizes the filtering function of the sample liquid in the sample bin 51.
When the heating type sample deproteinization removing and filtering device is used for treating sample liquid, firstly, the sample liquid to be treated is added into a sample bin 51, a filter element is installed, a deproteinization device 5 is installed on a reagent strip tray 31, a tray module 3 is placed at a heating position, a heating module 4 is controlled to be heated up, the heating module is ascended to wrap the deproteinization device 5 to heat the deproteinization device 5, and after heating is finished, a heating body 41 is controlled to descend; and moving the reagent strip tray 31 to a film pressing position, controlling the pressing block 22 to be installed and descend at a fixed speed, realizing a filtering process, and lifting the pressing block 22 after filtering is finished to take out the filter element barrel 53 to obtain sample liquid.
The embodiments of the present invention have been described in detail, but the description is only for the preferred embodiments of the present invention and should not be construed as limiting the scope of the present invention. All equivalent changes and modifications made within the scope of the present invention shall fall within the scope of the present invention.
Claims (8)
1. Heating type sample removes miscellaneous protein filter equipment which characterized in that: the film pressing device comprises a tray module, a film pressing module and a heating module, wherein the film pressing module is arranged above the tray module; the tray module, the film pressing module and the heating module are all arranged on the mounting frame;
the tray module comprises a reagent strip tray and one or more reagent strips detachably fixed on the reagent strip tray, and the reagent strips are provided with a protein removing device;
the film pressing module comprises a pressing block and a telescopic device for controlling the pressing block to move up and down, and the pressing block can apply downward pressure to the protein removing device;
the telescopic device is fixed on the film pressing frame, the film pressing frame comprises a film pressing top plate and two film pressing side plates arranged at two ends of the film pressing top plate, the fixed end of the telescopic device is fixedly arranged on the film pressing top plate, and the moving end of the telescopic device is connected with the pressing block;
the telescopic device comprises a stepping motor, a synchronous wheel set and a lead screw, the lower end of the lead screw is connected with the pressing block, the other end of the lead screw penetrates through the film pressing top plate to be connected with the synchronous wheel set, and the stepping motor is connected with the synchronous wheel set;
a film pressing guide rail is arranged on the film pressing frame and is arranged on the film pressing side plates or on a film pressing back plate between the two film pressing side plates, a pressing block guide groove is arranged on the pressing block, and the position of the pressing block guide groove is matched with that of the film pressing guide rail;
the heating module comprises a heating body and a heating sheet, the heating sheet is arranged on the side face of the heating body, one or more heating holes are formed in the heating body, and the protein removing device can be inserted into the heating holes.
2. The heated sample-removing impure protein filtration device of claim 1, wherein: and horizontal moving devices are arranged at two ends of the tray module.
3. The heated sample-removing impure protein filtration device of claim 2, wherein: horizontal migration device includes horizontal guide, horizontal slider, horizontal gear and horizontal migration motor, horizontal slider with horizontal guide cooperatees, horizontal slider bottom is equipped with the rack, the symmetry is equipped with on the mounting bracket horizontal guide, reagent strip tray both ends fixedly connected with horizontal slider, horizontal gear sets up mounting bracket one side, can with the rack cooperation on the horizontal slider, horizontal gear is connected with the horizontal migration motor.
4. The heated sample-removing hetero-protein filtration device of any one of claims 1-3, wherein: lifting devices are arranged at two ends of the heating module;
the heating module is provided with a heating sheet, and the heating sheet is arranged on the outer side of the heating module;
and a temperature probe is arranged in the heating body.
5. The heated sample-removing hetero-protein filtration device of claim 4, wherein: the lifting device comprises a vertical guide rail, a vertical sliding block, a vertical gear and a vertical moving motor, wherein the vertical sliding block is matched with the vertical guide rail, a rack is arranged on the vertical sliding block, the vertical guide rail is symmetrically arranged on the mounting rack, two ends of the heating body are fixedly connected with the vertical sliding block, the vertical gear is arranged on one side of the mounting rack and can be matched with the rack on the vertical sliding block, and the vertical gear is connected with the vertical moving motor.
6. The heated sample-removing hetero-protein filtration device of claim 4, wherein: the lifting device comprises guide grooves, guide wheels, a rotating shaft, an inner connecting rod and a lifting motor mechanism, the guide grooves are symmetrically arranged on the mounting frame, the guide wheels are arranged at two ends of the heating body, the guide wheels are embedded in the guide grooves, the rotating shaft is rotatably arranged on the mounting frame, the rotating shaft is connected with the heating body through the inner connecting rod, and the rotating shaft is connected with the lifting motor mechanism;
the inner connecting rod comprises a first connecting rod and a second connecting rod which are hinged, the other end of the first connecting rod is connected with the rotating shaft, and the other end of the second connecting rod is hinged with the heating body.
7. The heated sample-removing hetero-protein filtration device of claim 6, wherein: the lifting motor mechanism comprises a driving motor, a short swing arm, a long swing arm and a motor connecting rod, the short swing arm and the long swing arm are hinged to two ends of the motor connecting rod respectively, the other end of the short swing arm is connected with the driving motor, and the other end of the long swing arm is connected with the rotating shaft.
8. The heated sample-removing impure protein filtration device of claim 1, wherein: still include position sensor and contact, position sensor is fixed to be set up on the die pressing frame, the contact sets up on the briquetting, the contact can block position sensor.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202011213031.7A CN112023701B (en) | 2020-11-04 | 2020-11-04 | Heating type sample impurity-removing protein filtering device |
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| CN202011213031.7A CN112023701B (en) | 2020-11-04 | 2020-11-04 | Heating type sample impurity-removing protein filtering device |
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| CN112023701B true CN112023701B (en) | 2021-02-12 |
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| CN113567224B (en) * | 2021-01-29 | 2022-09-06 | 广东润鹏生物技术有限公司 | Heating device and to-be-heated member |
| CN112743316B (en) * | 2021-02-04 | 2022-05-24 | 苏州长光华医生物医学工程有限公司 | Device for conveniently loading reagent card |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2004020983A2 (en) * | 2002-06-06 | 2004-03-11 | Millipore Corporation | Ultrafiltration device for drug binding studies |
| CN104164511A (en) * | 2014-08-25 | 2014-11-26 | 天津喜诺生物医药有限公司 | Aspergillus quantitative detection fluorescence PCR (polymerase chain reaction) kit |
| CN105521712A (en) * | 2016-01-12 | 2016-04-27 | 天津喜诺生物医药有限公司 | Heatable filter used for removing impure proteins |
| CN106867883A (en) * | 2017-03-03 | 2017-06-20 | 苏州工业园区铂纳医学科技有限公司 | A kind of automatic instrument for extracting nucleic acid |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN208852538U (en) * | 2018-09-07 | 2019-05-14 | 卡替（上海）生物技术股份有限公司 | A kind of detection of cord blood stem cell and screening are with isolating and purifying device |
-
2020
- 2020-11-04 CN CN202011213031.7A patent/CN112023701B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2004020983A2 (en) * | 2002-06-06 | 2004-03-11 | Millipore Corporation | Ultrafiltration device for drug binding studies |
| CN104164511A (en) * | 2014-08-25 | 2014-11-26 | 天津喜诺生物医药有限公司 | Aspergillus quantitative detection fluorescence PCR (polymerase chain reaction) kit |
| CN105521712A (en) * | 2016-01-12 | 2016-04-27 | 天津喜诺生物医药有限公司 | Heatable filter used for removing impure proteins |
| CN106867883A (en) * | 2017-03-03 | 2017-06-20 | 苏州工业园区铂纳医学科技有限公司 | A kind of automatic instrument for extracting nucleic acid |
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