CN112023095A - Sample counting machine, target plate inactivation device and target plate inactivation method - Google Patents
Sample counting machine, target plate inactivation device and target plate inactivation method Download PDFInfo
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- CN112023095A CN112023095A CN202010969374.XA CN202010969374A CN112023095A CN 112023095 A CN112023095 A CN 112023095A CN 202010969374 A CN202010969374 A CN 202010969374A CN 112023095 A CN112023095 A CN 112023095A
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- space
- atomizer
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- 230000002779 inactivation Effects 0.000 title claims abstract description 94
- 238000000034 method Methods 0.000 title claims abstract description 10
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 41
- 239000007788 liquid Substances 0.000 claims abstract description 15
- 230000000415 inactivating effect Effects 0.000 claims abstract description 3
- 239000003595 mist Substances 0.000 claims description 31
- 238000007789 sealing Methods 0.000 claims description 26
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 6
- 239000006199 nebulizer Substances 0.000 claims description 5
- 239000012530 fluid Substances 0.000 claims 1
- 244000005700 microbiome Species 0.000 abstract description 8
- 230000009849 deactivation Effects 0.000 abstract description 5
- 238000001514 detection method Methods 0.000 abstract description 5
- 238000012864 cross contamination Methods 0.000 abstract description 2
- 230000000813 microbial effect Effects 0.000 abstract 1
- 238000001840 matrix-assisted laser desorption--ionisation time-of-flight mass spectrometry Methods 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 3
- 238000001819 mass spectrum Methods 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 150000002500 ions Chemical class 0.000 description 2
- 238000004659 sterilization and disinfection Methods 0.000 description 2
- 239000013589 supplement Substances 0.000 description 2
- 150000001298 alcohols Chemical class 0.000 description 1
- 238000000889 atomisation Methods 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000012512 characterization method Methods 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000002538 fungal effect Effects 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 238000000816 matrix-assisted laser desorption--ionisation Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 241000894007 species Species 0.000 description 1
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
- A61L2/16—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor using chemical substances
- A61L2/22—Phase substances, e.g. smokes, aerosols or sprayed or atomised substances
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
- A61L2/26—Accessories or devices or components used for biocidal treatment
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Epidemiology (AREA)
- Chemical & Material Sciences (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Animal Behavior & Ethology (AREA)
- Analytical Chemistry (AREA)
- Pathology (AREA)
- Immunology (AREA)
- General Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Apparatus For Disinfection Or Sterilisation (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The invention discloses a sample counting machine, a target plate inactivation device and a target plate inactivation method. Target slot, sealed cowling, atomizer and pump are all installed in the frame, and the sealed cowling forms the inactivation space with the cooperation of target slot, and the atomizer is used for providing the inactivation fog, and the pump is used for providing power drive inactivation fog and discharges through the gas outlet after air inlet moves to the inactivation space to reach the purpose that utilizes alcohol fog to carry out the microbial deactivation to the target surface. According to the invention, the atomizer is arranged to atomize the inactivation liquid, so that the inactivation liquid is driven to inactivate the target plate, the purpose of inactivating microorganisms is achieved, cross contamination is avoided, and the detection accuracy is obviously improved.
Description
Technical Field
The invention relates to sample pretreatment, in particular to a spotting machine, a target plate inactivation device and a target plate inactivation method.
Background
The operating principle of MALDI-TOF-MS is that matrix-assisted laser desorption ionization is utilized to analyze components on the surface of a sample to be detected into ions, and the ions with different mass-to-charge ratios (m/z) are separated through a flight time mass analyzer according to the length of the flight time to form mass spectrum peaks (mass spectrograms) with different intensities. In the field of microorganism identification, a bacterial or fungal mass spectrum database is established by using a standard strain and a strain with clear source typing, and the mass spectrum of an unknown strain is compared with the database by using MassLynx software (or other related software), so that the species of a microorganism to be detected can be determined. Compared with other microorganism detection technologies, the MALDI-TOF-MS technology has obvious high efficiency and accuracy, and the cost of each experiment is very low, so that the MALDI-TOF-MS technology becomes a popular method for microorganism identification.
The sample is usually immobilized on a target plate and then sent to MALDI-TOF-MS for analysis and characterization. However, the current operation of fixing the sample on the target plate is generally performed manually, which is not only inefficient, but also has low spotting precision and even affects the final analysis result of the mass spectrometer, and during the operation, microorganisms in the environment are easily introduced into the target plate, thereby affecting the detection result.
Disclosure of Invention
Aiming at the defects of the prior art, the invention provides a sample applicator, a target plate inactivation device and a target plate inactivation method, which aim to solve the problems that the artificial sample application efficiency is low, and microorganisms in the environment are attached to the target plate to influence the detection result.
In order to achieve the purpose, the technical scheme adopted by the application is as follows: the target plate inactivation device comprises a rack, a target groove for mounting a target plate, a sealing cover for covering the target groove, an atomizer and a pump, wherein the target groove, the sealing cover, the atomizer and the pump are all mounted on the rack, the sealing cover and the target groove are matched to form an inactivation space, and the wall of the inactivation space is provided with an air inlet and an air outlet; the atomizer is equipped with the inactivation liquid in to can the atomizing inactivation liquid, the pump is used for providing power drive inactivation fog and discharges through the gas outlet after the air inlet moves to the inactivation space.
Preferably, the sealing cap is movably connected with the frame.
Preferably, a liquid level sensor is installed in the atomizer.
Preferably, the nebulizer is an ultrasonic nebulizer.
Preferably, the inactivation solution is alcohol.
Preferably, the inactivation space formed by the sealing cover and the target groove is provided with a slit.
Preferably, the target plate inactivation device further comprises a tail gas treatment bottle, wherein the tail gas treatment bottle is connected with the gas outlet and is used for collecting the inactivation mist discharged through the inactivation space.
Preferably, the tail gas treatment bottle is filled with water.
Preferably, the pump is connected with the tail gas treatment bottle and is used for providing power to drive the inactivation mist in the inactivation space to be discharged into the tail gas treatment bottle through the air outlet.
Preferably, the pump has two, one of them is connected with the atomizer for providing the deactivation fog that power drive atomizer produced and move to the inactivation space through the air inlet, and another is connected with the tail gas treatment bottle for providing the deactivation fog in the power drive inactivation space and discharge to the tail gas treatment bottle through the gas outlet.
A spotting machine comprising the target plate inactivation device.
Preferably, the sample counting machine further comprises an outer shell and a cabin door which are installed on the rack, the outer shell is fixed on the rack, the cabin door is rotatably connected with the outer shell, and the sealing cover is fixed on the cabin door.
Preferably, a gap is formed in the side wall of the sealing cover, the air inlet and the air outlet are formed in the shell, and the positions of the air inlet and the air outlet correspond to the positions of the gap.
A method of target plate inactivation, the method comprising:
(1) the target plate is placed in the target groove, the cabin door is covered, and the sealing cover covers the target groove under the cabin door, so that an inactivation space is formed.
(2) The atomizer atomizes alcohol by utilizing ultrasonic waves to generate alcohol mist, the pump provides power to drive the alcohol mist to move to the inactivation space through the air inlet, and simultaneously the alcohol mist in the inactivation space is driven to be discharged into the tail gas treatment bottle through the air outlet;
(3) the tail gas treatment bottle collects alcohol mist, and the alcohol mist is prevented from dissipating.
The invention provides a sample applicator, a target plate inactivation device and a target plate inactivation method, which have the following beneficial effects:
(1) according to the sample counting machine provided by the invention, the target plate is inactivated through the alcohol mist, and the atomizer, the pump, the sealing cover and other devices are used for enabling the alcohol mist to form circulating airflow in the closed space and to be uniformly distributed on the target point, so that the purpose of microorganism inactivation is achieved, meanwhile, cross contamination is avoided, and the accuracy of detection is obviously improved.
(2) According to the sample counting machine provided by the invention, the tail gas processor is arranged to collect the inactivated alcohol tail gas, so that a large amount of alcohol is prevented from being dissipated into the air, the use safety of the instrument and the use rate of the alcohol are improved, and the tail gas is treated by water, so that the cost is low and the environmental protection property is good.
(3) According to the sample applicator provided by the invention, the slit is arranged between the sealing cover and the target groove, so that the mechanical friction between the sealing cover and the target groove is avoided, and meanwhile, the slit can supplement airflow; the problem that the inactivation mist is directly discharged from the air outlet after entering the inactivation space through the air inlet due to the fact that the air exhaust speed is greater than the air inlet speed of the atomizer, and therefore the inactivation mist cannot reach the target plate for disinfection and inactivation is solved.
Drawings
FIG. 1 is a schematic structural diagram of a sample machine of the present invention;
FIG. 2 is a schematic view of the hidden enclosure and door of FIG. 1;
fig. 3 is a schematic view of the structure of the sealed space of the present invention.
Reference numerals in the drawings of the specification include:
a frame 1; a housing 11; a hatch door 12; a target groove 2; a seal cover 3; a notch 31; an atomizer 4; a first diaphragm pump 5; a second diaphragm pump 6; a tail gas treatment bottle 7; an air inlet 8; and an air outlet 9.
Detailed Description
The present invention will be further described with reference to the following drawings and examples, which are illustrative only and not intended to be limiting, and the scope of the present invention is not limited thereby.
In the present invention, the meaning of "a plurality" means two or more unless otherwise specifically defined. The terms "upper", "lower", "left", "right", "inner", "outer", "front", "rear", "upper", "lower", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are merely for convenience in describing and simplifying the description, but do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore cannot be construed as indicating or implying relative importance.
Furthermore, unless expressly stated or limited otherwise, the terms "disposed," mounted, "" connected, "and the like are to be construed broadly, as if the terms" fixedly "or" detachably connected, "or" integrally formed; the connection can be mechanical connection or electrical connection; the connection may be direct or indirect via an intermediate medium, and may be communication between the two elements or an interaction relationship between the two elements. The specific meanings of the above terms in the present invention can be understood by those skilled in the art according to specific situations.
Example 1
As shown in fig. 1, 2 and 3, the present invention provides a target plate inactivation device for a sample counting machine, which comprises a frame 1, a housing 11, a hatch 12, a target slot 2 for mounting a target plate, a sealing cover 3 for covering the target slot 2, a gap 31, an atomizer 4, a first diaphragm pump 5, a second diaphragm pump 6, an exhaust gas treatment bottle 7, an air inlet 8 and an air outlet 9. Shell 11, hatch door 12, target groove 2, sealed cowling 3, atomizer 4, tail gas treatment bottle 7 and a diaphragm pump 5 and No. two diaphragm pumps 6 all install in frame 1, and sealed cowling 3 forms the inactivation space with the cooperation of target groove 2, and air inlet 8 and gas outlet 9 set up on the wall in inactivation space.
The target groove 2 is slightly larger than the target plate and is used for placing the target plate, and a magnet is also arranged in the groove and is used for fixing the target plate placed in the groove. Cabin door 12 rotates with shell 11 to be connected, 3 fixed connection of sealed cowling are on the cabin door and set up in target groove 2 top, the spotting machine when inactivating the target plate, cabin door 12 rotates and drives sealed cowling 3 and moves to covering target groove 2 in order to form the inactivation space, sealed cowling 3 and target groove 2 contactless, leave the slit, mechanical friction between sealed cowling 3 and target groove 2 has been avoided, can be by slit supplementary air current simultaneously, avoid because the air exhaust rate is greater than atomizer air intake rate, thereby it directly can not reach the problem of target plate department disinfection inactivation to lead to inactivation fog to get into behind the inactivation space by the gas outlet discharge. The sealing cover 3 is provided with a notch 31, and the notch 31 is matched with the air inlet 8 and the air outlet 9 when the sealing cover 3 covers the target slot 2 to form a sealed space (the positions of the air inlet 8 and the air outlet 9 are opposite to the notch 31).
The inactivation liquid is equipped with in the atomizer 4, and the inactivation liquid of this embodiment is the alcohol, and atomizer 4 sets up in the side of target slot 2, is connected with air inlet 8, and atomizer 4 adopts ultrasonic atomization alcohol, is provided with liquid level sensor in it and is used for surveying liquid level height, and liquid level sensor transmits liquid level height information to control system to realize automatic liquid feeding function.
Tail gas treatment bottle 7 is connected with gas outlet 9, is equipped with water in the bottle, utilizes the alcohol to collect alcohol tail gas in the very easy characteristic of dissolving in water of alcohol, avoids a large amount of alcohol to lose in the air, adopts the lower water of price to collect alcohol tail gas and has not only reduced instrument use cost, more does benefit to the environmental protection simultaneously.
The second diaphragm pump 6 is connected with the tail gas treatment bottle 7 and used for providing power to drive the alcohol mist to be discharged into the tail gas treatment bottle 7 through the sealing cover gap 31 and the gas outlet 9. As the mist in the inactivation space is pumped out, negative pressure is generated in the inactivation space, and the mist generated by the atomizer 4 is sucked into the inactivation space, so that the supplement of the mist in the inactivation space is formed.
The first diaphragm pump 5 is connected with the atomizer 4 and used for providing power to drive the alcohol mist generated by the atomizer 4 to move into the inactivation space through the air inlet 8 and the sealing cover gap 31. A diaphragm pump 5 can improve the speed that the fog got into the deactivation space, can improve the deactivation effect.
The target plate inactivation apparatus shown in fig. 1, 2 and 3 operates as follows:
(1) after a power supply is switched on, a target plate is placed in a target groove 2, the sample applicator can start the target plate inactivation function according to a working instruction, a cabin door 12 is closed, and the cabin door 12 drives a sealing cover 3 to move to cover the target groove 2 so as to form an inactivation space;
(2) the atomizer 4 atomizes alcohol by ultrasonic waves to generate alcohol mist, the first diaphragm pump 5 provides power to drive the alcohol mist to move into the inactivation space through the air inlet 8 and the sealing cover gap 31, and the diaphragm pump 6 provides power to drive the alcohol mist in the inactivation space to move into the tail gas treatment bottle 7 through the sealing cover gap 31 and the air outlet 9;
thus, a complete work flow is completed.
It will be appreciated that the off-gas treatment bottle 7 collects incoming alcohol mist. Through using devices such as a diaphragm pump 5 and a diaphragm pump 6 to cooperate with an atomizer 4, a tail gas treatment bottle 7, a sealing cover and the like, alcohol mist airflow in a small space is controlled, so that alcohol is uniformly distributed on a target spot to inactivate the target plate. The tail gas treatment bottle 7 is arranged to treat the alcohol tail gas, so that when the alcohol mist soaks each target point, a large amount of alcohol is prevented from being dissipated into the air, and the safety and the use rate of the alcohol are improved.
Example 2
The difference between this embodiment and embodiment 1 lies in, only contains a diaphragm pump, and the diaphragm pump links to each other with tail gas treatment bottle 7 for provide power, drive alcohol fog through sealed breach 31 and gas outlet 9 and move to tail gas treatment bottle 7 in, because the diaphragm pump will inactivate the air or the alcohol fog in the space and discharge, thereby controlled the air current direction in the space that inactivates, atomizer 4 is the continuous fog that produces simultaneously, fog can get into the space that inactivates, and discharge by gas outlet 9, also can carry out abundant inactivation to the target plate. This embodiment can reduce a diaphragm pump, practices thrift the cost.
The above examples are provided only for illustrating the present invention and are not intended to limit the present invention. Changes, modifications, etc. to the above-described embodiments are intended to fall within the scope of the claims of the present invention, as long as they are in accordance with the technical spirit of the present invention.
Claims (14)
1. A target plate inactivation device is characterized by comprising a rack, a target groove for mounting a target plate, a sealing cover for covering the target groove, an atomizer and a pump, wherein the target groove, the sealing cover, the atomizer and the pump are all mounted on the rack, the sealing cover and the target groove are matched to form an inactivation space, and the wall of the inactivation space is provided with an air inlet and an air outlet; the atomizer is equipped with the inactivation liquid in to can the atomizing inactivation liquid, the pump is used for providing power drive inactivation fog and discharges through the gas outlet after the air inlet moves to the inactivation space.
2. The target plate inactivation device of claim 1, wherein the sealed enclosure is movably coupled to the frame.
3. The target plate inactivation device of claim 1, wherein a liquid level sensor is installed in the nebulizer.
4. The target plate inactivation device of claim 1, wherein the nebulizer is an ultrasonic nebulizer.
5. The target plate inactivation device of claim 1, wherein the inactivation fluid is alcohol.
6. The target plate inactivation device of claim 1, wherein the inactivation space formed by the sealing cap in cooperation with the target slot is slit.
7. The target plate inactivation device of claim 1, further comprising a tail gas treatment bottle connected to the gas outlet for collecting the inactivation mist exhausted through the inactivation space.
8. The target plate inactivation device of claim 7, wherein the off-gas treatment vial comprises water.
9. The target plate inactivation device of claim 7, wherein the pump is coupled to the off-gas treatment bottle for providing a motive force to drive the inactivation mist in the inactivation space to exit the off-gas treatment bottle via the gas outlet.
10. The target plate inactivation apparatus of claim 7, wherein the pump comprises two pumps, one of the two pumps is connected to the atomizer for providing power to drive the inactivation mist generated by the atomizer to move to the inactivation space through the air inlet, and the other pump is connected to the tail gas treatment bottle for providing power to drive the inactivation mist in the inactivation space to be discharged to the tail gas treatment bottle through the air outlet.
11. A printer, characterized in that the printer comprises a target plate inactivation device according to any of claims 1-10.
12. The spotting machine of claim 11 further comprising a housing mounted to the frame and a hatch pivotally connected to the housing, the seal housing being secured to the hatch.
13. The printer of claim 12, wherein the side wall of the sealing cap has a notch, and the air inlet and the air outlet are disposed on the housing, and the positions of the air inlet and the air outlet correspond to the position of the notch.
14. A method of inactivating a target plate, comprising:
(1) the target plate is placed in the target groove, the cabin door is covered, and the sealing cover covers the target groove under the cabin door, so that an inactivation space is formed.
(2) The atomizer atomizes alcohol by utilizing ultrasonic waves to generate alcohol mist, the pump provides power to drive the alcohol mist to move to the inactivation space through the air inlet, and simultaneously the alcohol mist in the inactivation space is driven to be discharged into the tail gas treatment bottle through the air outlet;
(3) the tail gas treatment bottle collects alcohol mist, and the alcohol mist is prevented from dissipating.
Priority Applications (1)
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CN202010969374.XA CN112023095B (en) | 2020-09-15 | 2020-09-15 | Sample application machine, target plate inactivating device and target plate inactivating method |
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CN202010969374.XA CN112023095B (en) | 2020-09-15 | 2020-09-15 | Sample application machine, target plate inactivating device and target plate inactivating method |
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CN112023095B CN112023095B (en) | 2024-06-28 |
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Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB189923690A (en) * | 1899-11-28 | 1900-01-20 | Richard Wayne Rice | Spraying Apparatus. |
GB1128245A (en) * | 1965-11-18 | 1968-09-25 | Karl Gustaf Engelbrekt Rosdahl | Sterilising method and apparatus therefor |
US6279836B1 (en) * | 1998-03-02 | 2001-08-28 | Ecolab Inc. | Portable unit and wall unit dispensers and method of dispensing with timer |
CN204411347U (en) * | 2015-01-09 | 2015-06-24 | 四川卫康科技有限公司 | Ionization type atomization bactericidal unit |
CN209237150U (en) * | 2018-08-29 | 2019-08-13 | 浙江医尔仁医疗科技有限公司 | Chinese and Western medicine targets cold spraying machine |
CN213048424U (en) * | 2020-09-15 | 2021-04-27 | 重庆拓谱生物工程有限公司 | Spotting machine and target plate inactivation device |
-
2020
- 2020-09-15 CN CN202010969374.XA patent/CN112023095B/en active Active
Patent Citations (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB189923690A (en) * | 1899-11-28 | 1900-01-20 | Richard Wayne Rice | Spraying Apparatus. |
GB1128245A (en) * | 1965-11-18 | 1968-09-25 | Karl Gustaf Engelbrekt Rosdahl | Sterilising method and apparatus therefor |
US3481689A (en) * | 1965-11-18 | 1969-12-02 | Karl Gustaf Engelbrekt Rosdahl | Method for disinfecting medical instruments and apparatus |
US6279836B1 (en) * | 1998-03-02 | 2001-08-28 | Ecolab Inc. | Portable unit and wall unit dispensers and method of dispensing with timer |
CN204411347U (en) * | 2015-01-09 | 2015-06-24 | 四川卫康科技有限公司 | Ionization type atomization bactericidal unit |
CN209237150U (en) * | 2018-08-29 | 2019-08-13 | 浙江医尔仁医疗科技有限公司 | Chinese and Western medicine targets cold spraying machine |
CN213048424U (en) * | 2020-09-15 | 2021-04-27 | 重庆拓谱生物工程有限公司 | Spotting machine and target plate inactivation device |
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