CN112008091A - 一种高灵敏、低毒、具氧化模拟酶活性的金纳米簇制备方法与应用 - Google Patents

一种高灵敏、低毒、具氧化模拟酶活性的金纳米簇制备方法与应用 Download PDF

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CN112008091A
CN112008091A CN202010815635.2A CN202010815635A CN112008091A CN 112008091 A CN112008091 A CN 112008091A CN 202010815635 A CN202010815635 A CN 202010815635A CN 112008091 A CN112008091 A CN 112008091A
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李少光
姚宏
林新华
张晓颖
黄丽英
李光文
蔡佳松
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Abstract

本发明公开一种高灵敏、低毒、具氧化模拟酶活性的金纳米簇制备方法与应用，属于纳米生物材料技术领域。本发明涉及的金纳米簇的制备方法包括：配置L‑组氨酸溶液、抗坏血酸溶液、氯金酸溶液，再将上述溶液以一定比例和顺序混合，在所筛选条件下孵育，透析后得到组氨酸‑抗坏血酸‑金纳米簇金纳米团簇。本发明所得到的金纳米团簇探针具氧化模拟酶活性，在活细胞显像中显像具高灵敏特性，且荧光强度随探针浓度增加而升高，其对H2O2检测的线性范围和对Fe3+响应的浓度范围宽，其制备简便、低成本，具有多功能、高灵敏和高选择性检测特点和潜在生物活性显像作用，在生命分析领域有较为良好应用前景。

Description

一种高灵敏、低毒、具氧化模拟酶活性的金纳米簇制备方法与 应用
技术领域
本发明涉及纳米生物材料技术领域，设计并制备组氨酸（His）和抗坏血酸（AA）还原与共同保护的多功能金纳米团簇（His-AA-AuNCs）并研究其应用。
背景技术
细胞内过剩H2O2可导致多种炎症类型的发生，包括动脉粥样硬化、慢性阻塞性肺病和肝炎。因此对过氧化氢进行高灵敏度、高选择性的分析与检测是极为必要的。
目前对于过氧化氢检测方法有激光共聚焦显微检测、紫外分光光度计检测、高效液相色谱柱后衍生法、化学发光法等方法。激光共聚焦显微检测方法灵敏度高,时间短,尤其能够动态检测细胞内H2O2浓度的变化，但动态监测需要荧光探针低毒及抗光漂白作用强，因此，该方法对荧光探针有较高要求。紫外分光光度计检测法是一种快速简易的测定方法,所用试剂价格便宜, 通过制作标准曲线, 可以准确定量测定细胞内H2O2的浓度,但其不足之处是灵敏度不高。
本发明设计并制备组氨酸（His）和抗坏血酸（AA）还原与共同保护的多功能金纳米团簇（His-AA-AuNCs），发现基于过氧化氢模拟酶性质用于高灵敏度和选择性检测Fe3+，以及检测阿霉素损伤后细胞内活性氧水平。
发明内容
本发明的目的在于提供一种具有过氧化氢模拟酶活性的金纳米簇制备方法与应用。
本发明所提供的金纳米团簇，形状为类球形，直径为1-5纳米，有明显的晶格条纹，记为His-AA-AuNCs。
本发明的目的是这样实现的，本发明所提出的金纳米团簇的制备方法，具体步骤如下:
(1)在组氨酸溶液中，依次加入氯金酸溶液、抗坏血酸溶液；
(2)将步骤(1)所得的混合溶液恒温孵育；
(3)将步骤(2)所得孵育后的溶液透析纯化，于低温避光保存。
步骤(1)中，混合溶液的流程为：在5 mL，0.2 mol/L组氨酸中，搅拌下依次加入2mL，23.16 mmol/L氯金酸、2 mL，10 mmol/L抗坏血酸。
步骤(2)中，恒温温度为60℃，孵育时间为2小时。
步骤(3)中，透析条件为500 Da透析袋透析24小时，低温保存温度为4℃。
本发明中，金纳米团簇的最佳荧光激发波长为388 nm，发射波长为481 nm，在365nm紫外灯照射下呈蓝绿色荧光。
本发明中，金纳米团簇作为过氧化物模拟酶，对H2O2的浓度检测呈线性正比关系，检测线性范围10-9.97×106 μM。
本发明中，基于Fe3+正催化的金纳米团簇，其Fe3+浓度在0.28-280 nM范围内与A/A0(652 nm处各个Fe3+和空白组对应的吸光度比值)呈线性正比关系，在此范围内，Fe3+可较大程度提高金纳米团簇的响应信号。
本发明上述的制备方法制得的具有过氧化氢模拟酶活性的金纳米团簇，其特征在于，形状为类球形，直径为1-5纳米，有明显的晶格条纹，记为His-AA-AuNCs。
本发明中，上述的金纳米团簇在制备作为生命分析领域的药物中的应用。
本发明中，金纳米团簇作为探针用于检测阿霉素损伤后细胞内活性氧水平，随着阿霉素损伤程度加大，细胞荧光成像亮度增加，且荧光强度随探针浓度增加而升高。
本发明的有益效果：本发明提供的金纳米团簇，具有多功能检测、高灵敏度和高选择性、简化和低成本等优点，拓宽了金纳米簇在生命分析领域的应用。
附图说明
图1为本发明金纳米团簇制备方法流程图。
图2为His-AA-AuNCs的荧光发射光谱图。
图3为His-AA-AuNCs的透射电子显微图，图中，A为较大视野下His-AA-AuNCs的透镜图，B为其中一个金纳米簇的透镜图。
图4为His-AA-AuNCs对H2O2浓度检测的标准曲线图。
图5为体系干扰实验图。
图6为基于Fe3+正催化的金纳米团簇，其Fe3+浓度的标准曲线图。
图7为 His-AA-AuNCs在系列浓度阿霉素损伤的HepG 2(肝癌)细胞中的细胞成像图。图中，a为阴性对照组，b-f分别为加入2.5μM、5μM、10μM、20μM、40μM阿霉素干预组。
具体实施方式
本发明所提出的金纳米团簇的制备方法，具体步骤如下（见图1）:
(1)在组氨酸溶液中，依次加入氯金酸溶液、抗坏血酸溶液；
(2)将步骤(1)所得的混合溶液恒温孵育；
(3)将步骤(2)所得孵育后的溶液透析纯化，于低温避光保存。
实施例1：
His-AA-AuNCs的制备，具体步骤如下：
在5 mL，0.2 mol/L组氨酸中，搅拌下依次加入2 mL、23.16 mmol/L氯金酸，2 mL、10mmol/L抗坏血酸，反应在60 ℃孵育2 h后取出，用500 Da透析袋透析24 h，去除未反应的小分子。将获得的His-AA-AuNCs放置于4 ℃冰箱低温避光保存备用。His-AA-AuNCs形状为类球形，直径为1-5纳米，有明显的晶格条纹，见图3。其荧光发射光谱图，见图2，His-AA-AuNCs的最佳发射波长为481 nm。
实施例2：
His-AA-AuNCs作为过氧化物模拟酶，对H2O2的浓度检测呈线性正比关系，见图4，其对H2O2的浓度检测的线性范围为10-9.97×106 μM。
实施例3：
Fe3+能催化分解过氧化氢，显著增加His-AA-AuNCs的过氧化物模拟酶活性。通过考察其他金属离子及其他干扰物质有无催化酶活性，进一步研究该检测***的选择性。测试了Cu2+、Ca2+、Na+、K+、Co2+、Zn2+、腺苷、组氨酸和多巴胺等物质与Fe3+吸光度值进行比较，见图5，说明基于His-AA-AuNCs的过氧化物模拟酶活性增加可特异性检测Fe3+。
实施例4：
基于Fe3+正催化的金纳米团簇，其Fe3+浓度在0.28-280 nM范围内与A/A0(652 nm处各个Fe3+和空白组对应的吸光度比值)呈线性正比关系，在此范围内，Fe3+可较大程度提高金纳米团簇的响应信号，见图6。
实施例5：
His-AA-AuNCs金纳米团簇在制备作为生命分析领域的药物中的应用，其特征在于，His-AA-AuNCs可作为探针用于检测阿霉素损伤后细胞内活性氧水平，见图7，随着阿霉素损伤程度加大，细胞荧光成像亮度增加，且荧光强度随探针浓度增加而升高。

Claims (7)

1.一种具有过氧化氢模拟酶活性的金纳米簇制备方法，其特征在于，步骤如下：
在组氨酸溶液中，依次加入氯金酸溶液、抗坏血酸溶液，获得混合溶液；
将步骤(1)所得的混合溶液恒温孵育；
将步骤(2)所得孵育后的溶液透析纯化，于低温避光保存。
2.根据权利要求1所述的制备方法，其特征在于，步骤(1)中所述的混合溶液的流程为：在5 mL，浓度为0.2 mol/L组氨酸中，搅拌下依次加入2 mL，23.16 mmol/L氯金酸、2 mL，10mmol/L抗坏血酸。
3.根据权利要求1所述的制备方法，其特征在于，步骤(2)中，恒温温度为60℃，孵育时间为2小时。
4.根据权利要求1所述的制备方法，其特征在于，步骤(3)中，透析条件为500 Da透析袋透析24小时，低温保存温度为4℃。
5.权利要求1-4任一所述的制备方法制得的具有过氧化氢模拟酶活性的金纳米团簇，其特征在于，形状为类球形，直径为1-5纳米，有明显的晶格条纹，记为His-AA-AuNCs。
6.权利要求5所述的金纳米团簇在制备作为生命分析领域的药物中的应用。
7.根据权利要求6所述的应用，其特征在于，金纳米团簇作为探针用于检测阿霉素损伤后细胞内活性氧水平，随着阿霉素损伤程度加大，细胞荧光成像亮度增加，且荧光强度随探针浓度增加而升高。
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CHENGZHI ZHENG等: "Study on the interaction between histidine-capped Au nanoclusters and bovine serum albumin with spectroscopic techniques", 《SPECTROCHIMICA ACTA PART A: MOLECULAR AND BIOMOLECULAR SPECTROSCOPY》 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113502158A (zh) * 2021-07-14 2021-10-15 桂林电子科技大学 金纳米簇的制备方法及其在胆红素和锌离子联级检测中的应用
CN113502158B (zh) * 2021-07-14 2023-03-14 桂林电子科技大学 金纳米簇的制备方法及其在胆红素和锌离子联级检测中的应用

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