CN111974470A - Device and method for realizing micro-droplet transportation on heterogeneous substrate - Google Patents
Device and method for realizing micro-droplet transportation on heterogeneous substrate Download PDFInfo
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- CN111974470A CN111974470A CN202010515704.8A CN202010515704A CN111974470A CN 111974470 A CN111974470 A CN 111974470A CN 202010515704 A CN202010515704 A CN 202010515704A CN 111974470 A CN111974470 A CN 111974470A
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/50273—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
- B01L2400/0454—Moving fluids with specific forces or mechanical means specific forces radiation pressure, optical tweezers
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Abstract
The invention discloses a device and a method for realizing micro-droplet transportation on a foreign substrate, wherein the device comprises a laser 1, an electronic shutter 2, a diaphragm 3, a laser reflector 4, a focusing objective 5, a lithium niobate chip 6, an observation objective 7, an optical filter 8, a CCD camera 9, a background light source 10, a three-dimensional transparent moving platform 11 and a foreign substrate 12. The method has the characteristics of controllable micro-droplet transportation direction, variable volume of transported liquid droplets, controllable distance between a lithium niobate chip and a heterogeneous substrate, controllable transportation distance and the like, and the whole process can be observed in real time. The technology can be applied to the collection, mixing and transportation of trace reagents, avoids the direct contact between a chip and micro liquid drops to be transported, and has important significance for the development of the fields of biological medicine, drug diagnosis, environmental monitoring, molecular biology and the like.
Description
Technical Field
The invention relates to a technology for realizing the control of micro-droplets on a foreign substrate, in particular to a device and a method for realizing the transport of the micro-droplets by utilizing the action of an electric field generated by irradiating a lithium niobate chip with laser on the micro-droplets on the foreign substrate.
Background
With the rapid development of microfluidic chip technology, micro-droplet manipulation has become a research hotspot in this field. The micro-droplet control technology is mainly applied to analysis and detection of trace samples in the processes of biology, chemistry, pharmacy and the like, and mainly relates to collection, mixing, transportation and the like of trace reagents. It has very important significance for the development of the fields of biological medicine, medical diagnosis, food sanitation, environmental monitoring, molecular biology and the like.
The 2003 literature (Monolithic membrane vessels and diaphragm pumps for reactive large-scale integration) reports a method for transporting micro-liquids using micro-air pump devices, which requires air blowing, is prone to liquid contamination, requires the preparation of complex microchannels and microchambers, has dead zones, and is not controllable in transport direction and distance, and is only suitable for the transport of continuous fluids.
In 2007, Hong X et al (Application of hydrophilic Surface with High additive Force in No. Lost Transport of Superampatic micro-droplets, 129, 1478-9, (2007)) uses a super-hydrophobic Surface to make an electromagnetic switch, and realizes the Transport of micro-droplets between an upper and a lower electrode plates under the combined action of magnetic field acting Force, micro-droplet gravity and the adhesion of PS nanotubes to micro-droplets. The method needs to manufacture a PS nanotube layer, has complex process and higher cost, the distance between two polar plates is fixed to be 2mm, the movement distance of the micro-droplets is limited, and the method can only complete the movement of the micro-droplets between the upper polar plate and the lower polar plate, namely the micro-droplets can not be transported in any direction in a two-dimensional plane.
Disclosure of Invention
The currently reported micro-droplet transportation method has many disadvantages, such as: the device such as a micro-channel, a micro-chamber and the like needs to be prepared, the cost is high, the processing technology is complex, and the dead volume is easy to form; the direction and distance of micro-droplet transportation are not controllable; the transport process cannot be controlled in real time; the micro liquid drop is in direct contact with the core equipment, and the volume of the transported micro liquid drop cannot be changed. Aiming at the problems, the invention provides a simple and easy micro-droplet transportation method, the transportation direction and distance of the micro-droplet are controllable, the micro-droplet is not directly stored on a lithium niobate chip, the pollution to the chip is avoided, the volume of the transported micro-droplet is variable, the micro-droplet is not required to be subjected to any previous treatment, the micro-droplet can be transported in any direction in a two-dimensional plane in an air environment, and the whole process is controllable in real time.
An apparatus for effecting droplet transport on a foreign substrate, comprising: the micro-droplet transport optical path is formed by a laser 1, an electronic shutter 2, a diaphragm 3, a laser reflector 4, a focusing objective 5, a lithium niobate chip 6, a foreign substrate 12 and a transparent fine-tuning three-dimensional translation table 11 in sequence; a background light source 10, a transparent fine-tuning three-dimensional translation table 11, a heterogeneous substrate 12, an observation objective lens 7, an optical filter 8 and a CCD camera 9 form a real-time observation light path in sequence, and micro-droplet transportation and observation are simultaneously carried out through two partially-overlapped light paths.
A method for realizing micro-droplet transportation on a foreign substrate is characterized in that: a heterogeneous substrate is used as a droplet storage device, the heterogeneous substrate and a lithium niobate chip form a space interlayer, laser can move in any direction on a two-dimensional plane, micro droplets can be driven to move synchronously in real time, the transport direction and the transport distance of the micro droplets are further controlled, and the micro droplets are freely transported on the two-dimensional plane for many times.
A method for realizing micro-droplet transportation on a foreign substrate is characterized in that: by adjusting the laser power, the distance between the lithium niobate chip and the foreign substrate can be effectively adjusted, and the transportation of micro-droplets is realized.
A method for realizing micro-droplet transportation on a foreign substrate is characterized in that: the micro-droplets are not in direct contact with the lithium niobate chip, so that the pollution to the chip is avoided.
A method for realizing micro-droplet transportation on a foreign substrate is characterized in that: the lithium niobate chip realizes the transportation of polar liquid and nonpolar liquid under the condition of no contact with micro-droplets on a foreign substrate.
Compared with the prior art, the invention has the advantages that: the heterogeneous substrate is used as a droplet storage device, and a single lithium niobate chip and the heterogeneous substrate form a space interlayer, so that the direct contact between the lithium niobate chip and the micro-droplet to be controlled is avoided, and the device has a simple structure and low cost; the method has the characteristics that the transport direction and the distance of the micro liquid drops are controllable, the distance between the lithium niobate chip and the foreign substrate is controllable, the volume of the micro liquid drops is controllable, the micro liquid drops are not in contact with the lithium niobate chip, and the like, and the whole process can be observed in real time.
Drawings
FIG. 1 is a schematic diagram of the overall structure of the device for transporting micro-droplets on a foreign substrate according to the present invention.
Fig. 2 is a schematic diagram of a specific structure of a space interlayer between a lithium niobate chip and a foreign substrate for realizing a micro-droplet transportation scheme on the foreign substrate according to the present invention.
Fig. 3 is a diagram of a transport process of an embodiment (example 1) of the present invention for implementing a micro-droplet transport scheme on a foreign substrate.
Fig. 4 is a diagram of a transport process of an embodiment (example 2) of the present invention for implementing a micro-droplet transport scheme on a foreign substrate.
Fig. 5 is a diagram of a transport process for one embodiment (example 3) of the present invention for implementing a micro-droplet transport scheme on a foreign substrate.
Detailed Description
The invention will be further illustrated by the following examples and figures
The invention discloses a device and a method for realizing micro-droplet transportation on a foreign substrate, wherein the device comprises: the micro-droplet transport optical path is formed by a laser 1, an electronic shutter 2, a diaphragm 3, a laser reflector 4, a focusing objective 5, a lithium niobate chip 6, a foreign substrate 12 and a transparent fine-tuning three-dimensional translation table 11 in sequence; a background light source 10, a transparent fine-tuning three-dimensional translation stage 11, a heterogeneous substrate 12, an observation objective lens 7, an optical filter 8 and a CCD camera 9 form a real-time observation light path in sequence.
The invention discloses a device and a method for realizing micro-droplet transportation on a foreign substrate, wherein the method comprises the following operation steps: guiding micro-droplets to be transported onto a heterogeneous substrate 12, placing the heterogeneous substrate on a transparent fine-tuning three-dimensional translation table, adjusting the transparent fine-tuning three-dimensional translation table to enable the micro-droplets to be transported to be located near the focus of a focusing objective lens, and capturing a clear object image by using a CCD camera; adjusting the power of a laser and the distance between a lithium niobate chip and a foreign substrate, opening an electronic shutter, reflecting laser light by a reflector to enter a focusing objective lens and focus the laser light on the lithium niobate chip so as to generate a space electric field, adjusting the position of a micro-droplet on the foreign substrate to enable the micro-droplet to be positioned near a laser point, then enabling the laser light to move in a two-dimensional plane according to any direction, and at the moment, enabling the micro-droplet on the foreign substrate to move synchronously along with the laser light to realize the transportation of the micro-droplet.
In order to effectively transport micro droplets, the laser 1 is required to irradiate the lithium niobate chip with laser light to effectively excite carriers, so the wavelength of the laser light is 400-500 nm, and the power of the laser light is 5-100 mW; the background light source 10 may use a halogen lamp; the magnification of the focusing objective lens 5 is 10-30 times; the magnification of the observation objective lens 7 is 3 to 5 times.
Combining the above and considering the cost and transportation effect of the components, the preferred range of each parameter is: the wavelength of the laser is 390-500 nm, and the background light source 7 is a halogen lamp; the magnification of the focusing objective lens is 10-30 times. The magnification of the observation objective lens 7 is 3-5 times, and all optical elements and electronic devices on the light path are fixed on the rigid connecting frame in order to ensure the correct light transmission and measurement accuracy.
The working principle of the scheme of the invention is as follows: the laser irradiates the lithium niobate chip to generate a space electric field on the surface of the lithium niobate chip, the distance between the lithium niobate chip and the foreign substrate is adjusted, when a certain distance is reached, the space electric field generated on the surface of the lithium niobate chip acts on micro liquid drops on the foreign substrate, then the laser is controlled by a computer to move along any direction, the liquid drops synchronously move along with the laser, the laser is turned off, the micro liquid drops stop moving, and thus the micro liquid drops on the foreign substrate can be transported, and the moving direction, the transporting distance and the distance between the lithium niobate chip and the foreign substrate are controllable.
Specific examples of the present invention for implementing a micro-droplet transportation scheme on a foreign substrate are given below, and the specific examples are only for illustrating the present invention in detail and do not limit the scope of the claims of the present application.
Example 1
A405 nm laser is used, the laser power is 8.25mW, a halogen lamp is selected as a background light source, the magnification of a focusing objective lens is 25 times, a nonpolar micro-droplet with the volume of 0.1 muL to be transported is guided to a foreign substrate, the distance between a lithium niobate chip and the foreign substrate is 3mm, a transparent fine-tuning three-dimensional translation platform is moved to enable the micro-droplet to be located near a laser spot, an electronic shutter is opened, the laser moves in any direction in a two-dimensional plane, the micro-droplet moves along with the laser, the electronic shutter is closed, the movement of the micro-droplet is also stopped, and the transportation of the micro-droplet is realized.
Example 2
A405 nm laser is used, the laser power is 64.25mW, a halogen lamp is selected as a background light source, the magnification of a focusing objective lens is 25 times, a nonpolar micro-droplet with the volume of 0.3 mu L to be transported is guided into a foreign substrate, the distance between a lithium niobate chip and the foreign substrate is 5.63mm, a transparent fine-tuning three-dimensional translation platform is moved to enable the micro-droplet to be located near a laser spot, an electronic shutter is opened, the laser moves in any direction in a two-dimensional plane, the micro-droplet moves along with the laser, the electronic shutter is closed, the movement of the micro-droplet is also stopped, and the transportation of the micro-droplet is realized.
Example 3
A405 nm laser is used, the laser power is 110.25mW, a halogen lamp is selected as a background light source, the magnification of a focusing objective lens is 25 times, polar micro liquid drops with the volume of 0.1 mu L to be transported are led into a foreign substrate, the distance between a lithium niobate chip and the foreign substrate is 7.51mm, a transparent fine adjustment three-dimensional translation platform is moved to enable the micro liquid drops to be located near laser spots, an electronic shutter is opened, laser moves in any direction in a two-dimensional plane, the micro liquid drops move along with the laser, the electronic shutter is closed, the movement of the micro liquid drops is also stopped, and the transportation of the micro liquid drops is achieved.
The above embodiments are further described in detail, it should be understood that the above embodiments are not intended to limit the present invention, and all equivalent modifications, equivalent substitutions, improvements, etc. within the spirit and principle of the present invention should be considered within the scope of the present invention.
Claims (5)
1. An apparatus for effecting droplet transport on a foreign substrate, comprising: the micro-droplet transport optical path is formed by a laser 1, an electronic shutter 2, a diaphragm 3, a laser reflector 4, a focusing objective 5, a lithium niobate chip 6, a foreign substrate 12 and a transparent fine-tuning three-dimensional translation table 11 in sequence; a background light source 10, a transparent fine-tuning three-dimensional translation stage 8, a heterogeneous substrate 12, an observation objective lens 7, an optical filter 8 and a CCD camera 9 form a real-time observation light path in sequence; and the simultaneous operation of micro-droplet transportation and observation is realized through two partially overlapped light paths.
2. A method for realizing micro-droplet transportation on a foreign substrate is characterized in that: a heterogeneous substrate is used as a droplet storage device, the heterogeneous substrate and a lithium niobate chip form a space interlayer, laser can move in any direction on a two-dimensional plane, micro droplets can be driven to move synchronously in real time, the transport direction and the transport distance of the micro droplets are further controlled, and the micro droplets are freely transported on the two-dimensional plane for many times.
3. The method of claim 2, wherein the method comprises: by adjusting the laser power, the distance between the lithium niobate chip and the foreign substrate can be effectively adjusted, and the transportation of micro-droplets is realized.
4. The method of claim 2, wherein the method comprises: the micro-droplets are not in direct contact with the lithium niobate chip, so that the pollution to the chip is avoided.
5. The method of claim 2, wherein the method comprises: the lithium niobate chip realizes the transportation of polar liquid and nonpolar liquid under the condition of no contact with micro-droplets on a foreign substrate.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115140933A (en) * | 2022-06-27 | 2022-10-04 | 河北工业大学 | Device and method for preparing optical fiber in real time |
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