CN111939599B - Method and device for preparing platelet-rich plasma - Google Patents
Method and device for preparing platelet-rich plasma Download PDFInfo
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- CN111939599B CN111939599B CN202010673284.6A CN202010673284A CN111939599B CN 111939599 B CN111939599 B CN 111939599B CN 202010673284 A CN202010673284 A CN 202010673284A CN 111939599 B CN111939599 B CN 111939599B
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- 210000004623 platelet-rich plasma Anatomy 0.000 title claims abstract description 90
- 238000000034 method Methods 0.000 title claims abstract description 26
- 210000004369 blood Anatomy 0.000 claims abstract description 133
- 239000008280 blood Substances 0.000 claims abstract description 133
- 239000012528 membrane Substances 0.000 claims abstract description 109
- 210000002381 plasma Anatomy 0.000 claims abstract description 50
- 238000002360 preparation method Methods 0.000 claims abstract description 48
- 239000011148 porous material Substances 0.000 claims abstract description 15
- 238000005374 membrane filtration Methods 0.000 claims abstract description 9
- 230000002572 peristaltic effect Effects 0.000 claims description 38
- 239000007788 liquid Substances 0.000 claims description 33
- 239000003146 anticoagulant agent Substances 0.000 claims description 16
- 229940127219 anticoagulant drug Drugs 0.000 claims description 16
- 238000010241 blood sampling Methods 0.000 claims description 12
- 239000002504 physiological saline solution Substances 0.000 claims description 9
- 239000012530 fluid Substances 0.000 claims description 4
- 238000001914 filtration Methods 0.000 claims description 3
- 238000005119 centrifugation Methods 0.000 abstract description 4
- 210000001772 blood platelet Anatomy 0.000 description 23
- 239000000463 material Substances 0.000 description 10
- 239000012503 blood component Substances 0.000 description 5
- 210000003743 erythrocyte Anatomy 0.000 description 4
- 210000000265 leukocyte Anatomy 0.000 description 4
- 238000000926 separation method Methods 0.000 description 3
- 102000004506 Blood Proteins Human genes 0.000 description 2
- 108010017384 Blood Proteins Proteins 0.000 description 2
- 230000001112 coagulating effect Effects 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000003204 osmotic effect Effects 0.000 description 2
- 229920005597 polymer membrane Polymers 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000001802 infusion Methods 0.000 description 1
- 238000001990 intravenous administration Methods 0.000 description 1
- 238000003466 welding Methods 0.000 description 1
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D17/00—Separation of liquids, not provided for elsewhere, e.g. by thermal diffusion
- B01D17/02—Separation of non-miscible liquids
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Abstract
The invention belongs to the technical field of platelet-rich plasma preparation, and particularly relates to a preparation method of platelet-rich plasma, which adopts a membrane filtration method, and in a closed sterile environment, whole blood is sequentially filtered through a first filter membrane with the pore diameter of 3-5 mu m and a second filter membrane with the pore diameter of less than 2 mu m, and the platelet-rich plasma is remained in a cavity between the first filter membrane and the second filter membrane, so that the platelet-rich plasma is prepared, and compared with the traditional centrifugation method for preparing the platelet-rich plasma, the preparation method is simpler and faster to operate and is easy to realize; in addition, the platelet-rich plasma with different concentrations can be prepared by mixing the plasma output by the plasma output tube with the platelet-rich plasma prepared by the preparation method according to a certain proportion. The invention also provides a platelet-rich plasma preparation device, so that the preparation method of the platelet-rich plasma is realized.
Description
Technical Field
The invention relates to a method and a device for preparing platelet-rich plasma, and belongs to the technical field of platelet-rich plasma preparation.
Background
At present, the Platelet Rich Plasma (PRP) on the market has few types of preparation sets, but basically adopts centrifugal tube type centrifugation to separate platelets in the plasma so as to prepare the platelet rich plasma.
The centrifugal tube type platelet-rich plasma (PRP) preparation kit is easy to realize on the product structure, but is complicated in the actual operation process, the influence of human factors on the prepared platelet-rich plasma is great, the repeatability is poor, and a better preparation effect can be achieved only by a great deal of practice of professionals.
In addition, the preparation of Platelet Rich Plasma (PRP) is usually an open operation process, and is easy to dye bacteria.
Disclosure of Invention
The invention aims to solve the technical problems that: the preparation method of the platelet-rich plasma is characterized in that the preparation method of the platelet-rich plasma is realized, and the operation process is simple.
According to the preparation method of the platelet-rich plasma, a membrane filtration method is adopted, whole blood is sequentially filtered through a first filter membrane with the pore diameter of 3-5 mu m and a second filter membrane with the pore diameter of less than 2 mu m in a closed sterile environment, and the platelet-rich plasma is remained in a cavity between the first filter membrane and the second filter membrane, so that the platelet-rich plasma is prepared.
The platelet diameter in the whole blood of human body is 2-3 mu m, the average diameter of red blood cells is about 9 mu m, and the diameter of white blood cells is 7-20 mu m, so the invention adopts a membrane filtration method to separate and prepare the platelet-rich plasma in the whole blood.
Compared with the traditional centrifugation method for preparing platelet-rich plasma (such as preparing platelet-rich plasma by adopting a centrifugal tube type platelet-rich plasma preparation set), the membrane filtration method provided by the invention has the advantages that the operation for separating and preparing the platelet-rich plasma is simpler and faster, and the implementation is easy.
Preferably, the whole blood is filtered by a first filter membrane and a second filter membrane in sequence to obtain blood plasma, and the blood plasma is mixed with the prepared platelet-rich blood plasma according to a certain proportion to prepare the platelet-rich blood plasma with different concentrations. It should be noted that, the concentration of the obtained platelet-rich plasma can be calculated, for example: platelets in the platelet-rich plasma prepared by the preparation method can be counted through blood component equipment, so that the concentration of the prepared platelet-rich plasma is calculated.
The invention relates to a platelet-rich plasma preparation device, which comprises a shell, a first filter membrane and a second filter membrane, wherein the first filter membrane and the second filter membrane are fixed in the shell, and the two filter membranes divide the inner cavity of the shell into three layers; one end of the shell is provided with a whole blood inlet and a blood outlet, and the other end is provided with a plasma outlet; the aperture of the first filter membrane is 3-5 mu m, and the first filter membrane is close to one side of the whole blood inlet; the pore size of the second filter membrane is smaller than 2 μm, and the second filter membrane is close to the side of the plasma outlet; the chamber between the two filter membranes is a platelet collection chamber; the whole blood inlet is connected with a whole blood input tube, and a peristaltic pump is arranged on the whole blood input tube; the blood outlet is connected with a blood output tube, and a first liquid stopping clamp is arranged on the blood output tube; the plasma outlet is connected with a plasma output pipe, and a second liquid stopping clamp is arranged on the plasma output pipe.
The working principle process of the preparation device of the invention is as follows:
according to the invention, the inner cavity of the shell is divided into a three-layer cavity structure through the first filter membrane and the second filter membrane, blood enters the first layer cavity of the shell from the whole blood inlet, the first filter membrane (the aperture of the membrane material is 3-5 mu m) between the first layer cavity and the second layer cavity can effectively block red blood cells and white blood cells in whole blood, blood components such as blood platelets and the like can effectively pass through the first filter membrane and enter the second layer cavity, and because the aperture of the membrane material of the second filter membrane between the second layer cavity and the third layer cavity is smaller than 2 mu m, blood platelets can be effectively blocked through the second filter membrane, and only plasma proteins, moisture and the like can penetrate the second filter membrane, so that platelet-rich plasma is left in the second layer cavity (namely a platelet collection cavity); after the first liquid stopping clamp is opened, the blood after separation treatment in the first layer of chamber is directly discharged from the blood outlet; after the second liquid stopping clamp is opened, the plasma is output through a plasma output pipe.
The shape of the preparation device is not limited, and the preparation device can be in various types such as flat shape, cylindrical shape and the like. The filter membrane (including the first filter membrane and the second filter membrane) can be a porous polymer membrane material, or can be a membrane material formed by adopting a laser perforation or ray perforation mode for the airtight material, and the form is not limited.
Preferably, the first filter membrane and the second filter membrane are fixed in the shell through the first support frame and the second support frame respectively.
Preferably, the first filter membrane is close to the membrane surface of the second filter membrane or the membrane surface of the second filter membrane close to the first filter membrane is fixedly provided with a supporting piece, and the first filter membrane and the second filter membrane can be separated through the supporting piece, so that the first filter membrane and the second filter membrane are prevented from being attached. Of course, the distance between the first filter membrane and the second filter membrane can be increased, and the first filter membrane and the second filter membrane can be prevented from being attached.
Preferably, a platelet-rich plasma output tube is arranged on the shell corresponding to the position of the platelet collection cavity; a third liquid stopping clamp is arranged on the platelet-rich plasma output pipe. And opening the third liquid stopping clamp, and outputting the platelet-rich plasma collected in the platelet collection cavity through a platelet-rich plasma output pipe, so as to obtain the required platelet-rich plasma. In actual operation, the needle of the intravenous infusion set can also be directly penetrated into the platelet collection cavity of the shell, so that the platelet-rich plasma is extracted.
Preferably, the plasma output pipe is connected with a first circulation bypass, the other end of the first circulation bypass is connected into the whole blood input pipe, and a first peristaltic pump is arranged on the first circulation bypass; the first circulation bypass is connected to the plasma output tube segment between the plasma outlet and the second fluid stop clamp. When the device is operated, the second liquid stopping clamp is closed, and the first peristaltic pump is started, so that the blood plasma flowing out of the blood plasma outlet can enter the preparation device again through the first circulation bypass and the whole blood inlet, and the preparation rate of the platelet-rich blood plasma is improved through one or more repeated circulation.
The whole blood input tube can be a whole blood conveying pipeline connected with the blood storage container, and the whole blood conveying pipeline is provided with the second peristaltic pump, so that the whole blood in the blood storage container can be directly conveyed into the preparation device through the second peristaltic pump because the whole blood in the blood storage container is added with the anticoagulant. The whole blood input tube can also be a blood sampling tube, a third peristaltic pump is arranged on the blood sampling tube, an anticoagulant tube is connected to the blood sampling end of the blood sampling tube, and a fourth liquid stopping clamp is arranged on the anticoagulant tube; the anticoagulant line is located before the third peristaltic pump. The blood collected from the human body needs to be added with an anticoagulant in time to prevent the collected blood from coagulating, and the blood added with the anticoagulant is conveyed to the preparation device through the third peristaltic pump.
Preferably, the blood output tube is connected with a return pipeline for conveying blood back to the human body; the blood outlet is connected with the first peristaltic pump; in order to ensure the osmotic pressure balance between the blood conveyed back to the human body in the return pipeline and the blood in the human body, the return pipeline is connected with a physiological saline pipeline, and a fifth liquid stopping clamp is arranged on the physiological saline pipeline. When the blood output by the blood output tube is required to be conveyed back to the human body, the first liquid stopping clamp is required to be closed, the fourth peristaltic pump is started, the fifth liquid stopping clamp is opened, physiological saline is added into the blood of the return conveying pipeline, and the blood is conveyed back to the human body through the fourth peristaltic pump.
Preferably, the blood output tube is connected with a second circulation bypass, the other end of the second circulation bypass is connected into the whole blood input tube, and a fifth peristaltic pump is arranged on the second circulation bypass; the second circulation bypass is connected to the blood output tube segment between the blood outlet and the first fluid stop clip. When the operation is carried out, the first liquid stopping clamp is closed, the fifth peristaltic pump is started, blood flowing out of the blood outlet enters the preparation device through the second circulation bypass and the whole blood inlet, and the preparation rate of platelet-rich plasma is improved through one or more repeated circulation.
Compared with the prior art, the invention has the following beneficial effects:
according to the invention, a membrane filtration method is adopted, and different blood components are fixed on a certain layer through two layers of filter membranes with different apertures, so that the separation effect is achieved, and the platelet-rich plasma in whole blood is separated and prepared. In addition, the platelet-rich plasma with different concentrations can be prepared by mixing the plasma output by the plasma output tube with the platelet-rich plasma prepared by the preparation method according to a certain proportion.
Drawings
FIG. 1 is a schematic diagram of a platelet rich plasma preparation apparatus according to an embodiment;
fig. 2 is a schematic structural diagram of a platelet rich plasma preparation apparatus according to the second embodiment.
In the figure: 1. a housing; 2. a whole blood inlet; 3. a blood collection pipeline; 4. a fourth liquid stop clamp; 5. an anticoagulant line; 6. a third peristaltic pump; 7. a second circulation bypass; 8. a fifth peristaltic pump; 9. a blood output tube; 10. a first liquid stop clamp; 11. a physiological saline line; 12. a fifth liquid stop clamp; 13. a return pipeline; 14. a fourth peristaltic pump; 15. a blood outlet; 16. a first filter membrane; 17. a first support frame; 18. a third liquid stop clamp; 19. platelet rich plasma output tube; 20. a second support frame; 21. a second filter membrane; 22. a support; 23. a plasma outlet; 24. a plasma output tube; 25. a second liquid stop clamp; 26. a first circulation bypass; 27. a first peristaltic pump; 28. a platelet collection chamber; 29. a second peristaltic pump; 30. a whole blood transfer line; 31. a blood storage container.
Detailed Description
Embodiments of the invention are further described below with reference to the accompanying drawings:
according to the preparation method of the platelet-rich plasma, a membrane filtration method is adopted, whole blood is sequentially filtered through a first filter membrane with the pore diameter of 3-5 mu m and a second filter membrane with the pore diameter of less than 2 mu m in a closed sterile environment, and the platelet-rich plasma is remained in a cavity between the first filter membrane and the second filter membrane, so that the platelet-rich plasma is prepared.
The platelet diameter in the whole blood of human body is 2-3 mu m, the average diameter of red blood cells is about 9 mu m, and the diameter of white blood cells is 7-20 mu m, so the invention adopts a membrane filtration method to separate and prepare the platelet-rich plasma in the whole blood.
Compared with the traditional centrifugation method for preparing platelet-rich plasma (such as preparing platelet-rich plasma by adopting a centrifugal tube type platelet-rich plasma preparation set), the membrane filtration method provided by the invention has the advantages that the operation for separating and preparing the platelet-rich plasma is simpler and faster, and the implementation is easy.
And filtering the whole blood sequentially through a first filter membrane and a second filter membrane to obtain plasma, and mixing the plasma with the prepared platelet-rich plasma according to a certain proportion to prepare the platelet-rich plasma with different concentrations. It should be noted that, the concentration of the obtained platelet-rich plasma can be calculated, for example: platelets in the platelet-rich plasma prepared by the preparation method can be counted through blood component equipment, so that the concentration of the prepared platelet-rich plasma is calculated.
As shown in fig. 1, in order to implement the above preparation method, the platelet-rich plasma preparation apparatus according to the first embodiment includes a housing 1, and a first filter membrane 16 and a second filter membrane 21 fixed in the housing 1, where the two filter membranes divide the inner cavity of the housing 1 into three layers; one end of the shell 1 is provided with a whole blood inlet 2 and a blood outlet 15, and the other end is provided with a plasma outlet 23; the pore size of the first filter membrane 16 is 3-5 mu m, and the pore size is close to one side of the whole blood inlet 2; the pore size of the second filter membrane 21 is less than 2 μm, which is on the side close to the plasma outlet 23; the chamber between the two filters is a platelet collection chamber 28; the whole blood inlet 2 is connected with a whole blood input tube, and a peristaltic pump is arranged on the whole blood input tube; the blood outlet 15 is connected with the blood output tube 9, and a first liquid stopping clamp 10 is arranged on the blood output tube 9; the plasma outlet 23 is connected with a plasma output tube 24, and a second liquid stopping clamp 25 is arranged on the plasma output tube 24.
The working principle process of the preparation device in this embodiment is as follows:
the inner cavity of the housing 1 of the embodiment is divided into a three-layer cavity structure by the first filter membrane 16 and the second filter membrane 21, blood enters the first layer cavity of the housing 1 from the whole blood inlet 2, the first filter membrane 16 (the pore diameter of the membrane material is 3-5 μm) between the first layer cavity and the second layer cavity can effectively block red blood cells and white blood cells in whole blood, blood components such as platelets can effectively pass through the first filter membrane 16 and enter the second layer cavity, and because the pore diameter of the membrane material of the second filter membrane 21 between the second layer cavity and the third layer cavity is smaller than 2 μm, platelets can be effectively blocked by the second filter membrane 21, only plasma proteins, moisture and the like can penetrate the second filter membrane 21, so that platelet-rich plasma stays in the second layer cavity (namely the platelet collection cavity 28); after the first liquid stopping clamp 10 is opened, the blood after separation treatment in the first layer chamber is directly discharged from the blood outlet 15; after opening the second stopper 25, plasma is delivered through the plasma delivery tube 24.
Compared with the traditional centrifugal tube type Platelet Rich Plasma (PRP) preparation set, the preparation device for preparing the platelet rich plasma is simpler and faster in operation and easy to realize.
In this embodiment:
the first filter membrane 16 and the second filter membrane 21 are respectively fixed in the shell 1 through the first supporting frame 17 and the second supporting frame 20; the ultrasonic welding support 22 is adopted on the membrane surface of the first filter membrane 16 close to the second filter membrane or the membrane surface of the second filter membrane 21 close to the first filter membrane, and the first filter membrane 16 and the second filter membrane 21 can be separated through the support 22, so that the first filter membrane 16 and the second filter membrane 21 are prevented from being attached.
A platelet-rich plasma output pipe 19 is arranged on the shell 1 corresponding to the position of the platelet collection cavity 28; a third fluid stop clamp 18 is mounted on the platelet rich plasma outlet tube 19. The third stopper 18 is opened and the platelet-rich plasma collected in the platelet collection chamber 28 is output through the platelet-rich plasma output tube 19, thereby obtaining the desired platelet-rich plasma.
The plasma output tube 24 is connected with a first circulation bypass 26, the other end of the first circulation bypass 26 is connected into the whole blood input tube, and a first peristaltic pump 27 is arranged on the first circulation bypass 26; the first circulation bypass 26 is connected to the plasma output tube section between the plasma outlet 23 and the second stopper clamp 25. In operation, the second liquid stop clamp 25 is closed, and the first peristaltic pump 27 is started, so that the blood plasma flowing out of the blood plasma outlet 23 can enter the preparation device again through the first circulation bypass 26 and the whole blood inlet 2, and the preparation rate of the platelet-rich blood plasma is improved through one or more repeated circulation.
The whole blood input tube may be a whole blood transfer line 30 connected to a blood storage container 31, and the whole blood transfer line 30 is provided with a second peristaltic pump 29, so that the whole blood in the blood storage container 31 can be directly transferred to the preparation apparatus by the second peristaltic pump 29 because the whole blood in the blood storage container 31 is added with an anticoagulant. The whole blood input tube can also be a blood sampling tube 3, a third peristaltic pump 6 is arranged on the blood sampling tube 3, an anticoagulant tube 5 is connected to the blood sampling end of the blood sampling tube 3, and a fourth liquid stopping clamp 4 is arranged on the anticoagulant tube 5; the anticoagulant line 5 is located before the third peristaltic pump 6. The blood collected from the human body needs to be added with an anticoagulant in time to prevent the collected blood from coagulating, and the blood after the anticoagulant addition is delivered to the preparation apparatus through the third peristaltic pump 6.
The blood output pipe 9 is connected with a return pipeline 13 for conveying blood back to the human body; the return pipeline 13 is connected to the blood output pipe section between the blood outlet 15 and the first liquid stopping clamp 10, and a fourth peristaltic pump 14 is arranged on the return pipeline 13; in order to ensure the osmotic balance between the blood returned to the human body and the blood in the human body in the return pipeline 13, the physiological saline pipeline 11 is connected to the return pipeline 13, and the fifth liquid stopping clamp 12 is arranged on the physiological saline pipeline 11. When the blood output by the blood output tube 9 is required to be conveyed back to the human body, the first liquid stopping clamp 10 is closed, the fourth peristaltic pump 14 is started, the fifth liquid stopping clamp 12 is opened, physiological saline is added into the blood of the return pipeline 13, and the blood is conveyed back to the human body through the fourth peristaltic pump 14.
The blood output tube 9 is connected with a second circulation bypass 7, the other end of the second circulation bypass 7 is connected into the whole blood input tube, and a fifth peristaltic pump 8 is arranged on the second circulation bypass 7; the second circulation bypass 7 is connected to the blood outlet tube section between the blood outlet 15 and the first stopper clamp 10. In operation, the first liquid stopping clamp 10 is closed, the fifth peristaltic pump 8 is started, and then the blood flowing out of the blood outlet 15 enters the preparation device through the second circulation bypass 7 and the whole blood inlet 2, and the preparation rate of platelet-rich plasma is improved through one or more repeated circulation.
The shape of the preparation device described in this embodiment is not limited, and may be various types such as flat, cylindrical, etc. The filter membrane (including the first filter membrane 16 and the second filter membrane 21) may be a porous polymer membrane material, or may be a membrane material formed by laser perforation or ray perforation of an airtight material, and the form is not limited.
As shown in fig. 2, the other platelet-rich plasma preparation apparatus for realizing the above preparation method of the second embodiment has the same structure as that of the first embodiment, except that the whole blood inlet 2 and the blood outlet 15 are located below the upper and lower plasma outlets 23 in the present embodiment, which corresponds to the upside-down overall structure of the first embodiment. However, the working principle of the present embodiment is exactly the same as that of the first embodiment, and will not be described here.
Claims (9)
1. A preparation method of platelet-rich plasma is characterized by comprising the following steps: the method comprises the steps of sequentially filtering whole blood through a first filter membrane with the aperture of 3-5 mu m and a second filter membrane with the aperture of less than 2 mu m in a closed sterile environment by adopting a membrane filtration method, and keeping platelet-rich plasma in a cavity between the first filter membrane and the second filter membrane to obtain platelet-rich plasma;
the preparation device of the platelet-rich plasma comprises a shell (1), a first filter membrane (16) and a second filter membrane (21) which are fixed in the shell (1), wherein the two filter membranes divide the inner cavity of the shell (1) into three layers; one end of the shell (1) is provided with a whole blood inlet (2) and a blood outlet (15), and the other end is provided with a plasma outlet (23); the pore diameter of the first filter membrane (16) is 3-5 mu m, and the pore diameter is close to one side of the whole blood inlet (2); the pore size of the second filter membrane (21) is smaller than 2 mu m, and the second filter membrane is close to one side of the plasma outlet (23); the chamber between the two filter membranes is a platelet collection cavity (28); the whole blood inlet (2) is connected with a whole blood input tube, and a peristaltic pump is arranged on the whole blood input tube; the blood outlet (15) is connected with the blood output tube (9), and a first liquid stopping clamp (10) is arranged on the blood output tube (9); the plasma outlet (23) is connected with a plasma output pipe (24), and a second liquid stopping clamp (25) is arranged on the plasma output pipe (24).
2. The method for preparing platelet rich plasma according to claim 1, wherein: and filtering the whole blood sequentially through a first filter membrane and a second filter membrane to obtain plasma, and mixing the plasma with the prepared platelet-rich plasma according to a certain proportion to prepare the platelet-rich plasma with different concentrations.
3. The method for preparing platelet rich plasma according to claim 1, wherein: the first filter membrane (16) and the second filter membrane (21) are respectively fixed in the shell (1) through the first support frame (17) and the second support frame (20).
4. A method of preparing platelet rich plasma according to claim 1 or 3, wherein: a support (22) is fixed on the membrane surface of the first filter membrane (16) close to the second filter membrane or the membrane surface of the second filter membrane (21) close to the first filter membrane.
5. The method for preparing platelet rich plasma according to claim 1, wherein: a platelet-rich plasma output pipe (19) is arranged on the shell (1) corresponding to the position of the platelet collection cavity (28); a third liquid stopping clamp (18) is arranged on the platelet-rich plasma output pipe (19).
6. The method for preparing platelet rich plasma according to claim 1, wherein: the plasma output tube (24) is connected with a first circulation bypass (26), the other end of the first circulation bypass (26) is connected into the whole blood input tube, and a first peristaltic pump (27) is arranged on the first circulation bypass (26); the first circulation bypass (26) is connected to the plasma outlet line segment between the plasma outlet (23) and the second stopper clamp (25).
7. The method for preparing platelet rich plasma according to claim 1, 3, 5 or 6, wherein: the whole blood input pipe is a whole blood conveying pipeline (30) connected with a blood storage container (31), and a second peristaltic pump (29) is arranged on the whole blood conveying pipeline (30);
or the whole blood input tube is a blood sampling pipeline (3), and a third peristaltic pump (6) is arranged on the blood sampling pipeline (3); an anticoagulant pipeline (5) is connected to the near blood sampling end of the blood sampling pipeline (3), and a fourth liquid stopping clamp (4) is arranged on the anticoagulant pipeline (5); an anticoagulant line (5) is located before the third peristaltic pump (6).
8. The method for preparing platelet rich plasma according to claim 1, 3, 5 or 6, wherein: a blood output pipe (9) is connected with a return pipeline (13) for conveying blood back to the human body, and a fourth peristaltic pump (14) is arranged on the return pipeline (13); the blood delivery pipeline (13) is connected to a blood delivery pipeline section between the blood outlet (15) and the first liquid stopping clamp (10); the physiological saline pipeline (11) is connected to the return pipeline (13), and a fifth liquid stopping clamp (12) is arranged on the physiological saline pipeline (11).
9. The method for preparing platelet rich plasma according to claim 1, 3, 5 or 6, wherein: the blood output tube (9) is connected with a second circulation bypass (7), the other end of the second circulation bypass (7) is connected into the whole blood input tube, and a fifth peristaltic pump (8) is arranged on the second circulation bypass (7); the second circulation bypass (7) is connected to the blood outlet line segment between the blood outlet (15) and the first fluid stop clamp (10).
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