CN111937677A - Ecological cultivation method of Guangxi indigenous wild purple lucid ganoderma - Google Patents
Ecological cultivation method of Guangxi indigenous wild purple lucid ganoderma Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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Abstract
The invention discloses an ecological cultivation method of Guangxi indigenous wild purple lucid ganoderma, relating to the technical field of lucid ganoderma cultivation, and the key points of the technical scheme are as follows: the method comprises the following steps: s1, preparing three grades of culture media; s2, collecting wild Ganoderma species grown in subtropical zone, making into strains, and sequentially inoculating the strains into three grades of culture medium to obtain three grades of strains; s3, inoculating the third-level seeds onto a log to prepare a fungus stick; s4, preparing a ground cultivation compost; s5, selecting a tea-oil tree forest land as a planting land, and horizontally placing the fungus sticks in the planting holes to enable the planted fungus sticks to naturally grow into ganoderma sinense. The ecological cultivation method can simulate wild cultivation to produce Ganoderma lucidum with rich nutrient substances and good quality, and adopts resource dominant checked wood, green sentry wood and mulberry twig in subtropical regions as cultivation raw materials, so that the dominant resources in the subtropical regions are fully utilized, and the cultivation cost of Ganoderma lucidum is reduced; meanwhile, a tea-oil tree woodland is selected for segment-wood bionic planting, and the potential of the woodland is further excavated.
Description
Technical Field
The invention relates to the technical field of ganoderma lucidum cultivation, in particular to an ecological cultivation method of Guangxi indigenous wild purple ganoderma lucidum.
Background
The purple lucid ganoderma is also named as Chinese lucid ganoderma and immortal grass, has mild nature and no toxicity, and is the top grade. Wild ganoderma lucidum has limited national yield and is mainly distributed in regions such as Guangdong, Guangxi, Hainan, Sichuan, Guizhou, Yunnan, Shaanxi and the like. Guangxi belongs to subtropical regions, and the wild ganoderma sinense in Guangxi is abundant in resources and high in quality. The wild Ganoderma of Guangxi contains multiple active ingredients, has effects of regulating immunity, resisting tumor, resisting cancer, improving blood circulation, nourishing brain and promoting urination, is beneficial and harmless to human body, and is suitable for long-term health promotion.
Because the active cost value of the wild ganoderma lucidum in Guangxi province is high, the mass production of the wild ganoderma lucidum cannot meet the market demand, and because the yield of the wild ganoderma lucidum is low, people adopt an artificial common method to cultivate the ganoderma lucidum in the prior art.
At present, the main cultivation mode of ganoderma sinense is cultivation of bag materials in a room (shed). The ganoderma lucidum cultivated by indoor bag material has insufficient accumulation of effective nutrient components due to short growth time (generally 1-2 months), and has low nutrient value; in addition, the purple lucid ganoderma cultivated by indoor bag materials needs to occupy living space or needs to be put up in the field for shading, so that the production cost is high, and the development of purple lucid ganoderma planting is not facilitated.
Disclosure of Invention
The invention aims to provide an ecological cultivation method of Guangxi indigenous wild purple lucid ganoderma, which can cultivate lucid ganoderma with rich nutrient substances and excellent quality by imitating wild cultivation, and takes resource dominant checked wood, green holly wood and mulberry twig in subtropical regions as cultivation raw materials, thereby fully utilizing the dominant resources in the subtropical regions and reducing the cultivation cost of the purple lucid ganoderma; meanwhile, a tea-oil tree woodland is selected for segment-wood bionic planting, and the potential of the woodland is further excavated.
The technical purpose of the invention is realized by the following technical scheme: an ecological cultivation method of Guangxi indigenous wild purple lucid ganoderma specifically comprises the following steps:
s1, selecting and processing culture medium, preparing a first-class culture medium by adopting fresh green roughwood leaves, fresh mako leaves, peptone, sugarcane juice, agar and water, preparing a second-class culture medium by adopting miscellaneous wood chips, bagasse and gypsum powder, and preparing a third-class culture medium by adopting mako wood chips, wheat bran, sugar and gypsum powder;
s2, strain preparation and strain inoculation, wherein the method comprises the steps of collecting wild ganoderma lucidum varieties growing in subtropical regions, separating the collected wild ganoderma lucidum varieties to prepare strains, inoculating the strains into a first-class strain culture medium for culture to obtain first-class strains, inoculating the first-class strains into a second-class strain culture medium for culture to obtain second-class strains, and inoculating the second-class strains into a third-class strain culture medium to obtain third-class strains;
s3, preparing a bacterium rod, namely inoculating the third-level seeds in the step S2 to the sterilized log for culture to prepare the bacterium rod;
s4, preparing ground cultivation compost, mixing the green hillock, the mulberry branch, the wood dust, the bagasse and the gypsum powder in proportion to prepare the ground cultivation compost for planting;
s5, selecting a ganoderma lucidum planting field to plant, taking the camellia oleifera forest land with closed inter-plant as a planting field, punching a plurality of rows of planting holes in the space between two adjacent rows of camellia oleifera trees by using planting punching equipment, wherein the distance between each row is 50-60cm, then laying the ground planting compost prepared in the step S4 in the planting holes, flatly placing the fungus sticks prepared in the step S3 in the planting holes, covering fine soil on the surfaces of the fungus sticks, completing planting operation, and enabling the planted fungus sticks to naturally grow into ganoderma sinense.
Further, the size specification of the planting hole in step S5 is 25cm × 25cm × 20 cm.
Further, the mixture ratio of the first-class seed culture medium in the step S1 is as follows: 10-20 g of fresh green roughhaired holly leaves, 10-20 g of fresh gelia japonica leaves, 20-25 g of peptone, 30-40 g of sugarcane juice, 20-25 g of agar and 800ml of water.
Further, the weight ratio of the ingredients in the secondary seed culture medium in the step S1 is as follows: 60% of wood dust, 40% of bagasse and 1.5% of gypsum powder; the sawdust comprises the following components in parts by weight: 50% of green-hillock sawdust and 50% of mulberry branch sawdust.
Further, in the step S1, the weight ratio of the ingredients in the third-class seed culture medium is: 78% of wood chips, 10% of wheat bran, 1% of sugar and 1% of gypsum powder.
Further, the ground cultivation material in the step S4 comprises the following ingredients in parts by weight: 60% of green hillock wood, mulberry twig and bakelite sawdust, 38% of bagasse and 2% of gypsum powder.
Further, after the planting operation is completed in step S5, the camellia oleifera forest land is mowed and nurtured for 1 time in 2-3 months per year, and the planting land is periodically supplemented with the ganoderma lucidum growth nutrient solution.
In conclusion, the invention has the following beneficial effects:
1. the method comprises the following steps of (1) adopting a subtropical indigenous grown wild ganoderma variety, separating the collected wild ganoderma, and then respectively preparing a primary seed culture medium, a secondary seed culture medium and a tertiary seed culture medium to complete three-level strain cultivation processes of strains, so that the problems of single tree seeds and unreasonable cultivation material ingredients when one culture material is directly adopted can be avoided in the strain cultivation process, sufficient nutrient substances can be accumulated, and the cultivation of good-quality ganoderma is facilitated;
2. the method takes the resource dominant checked wood, the green roughcast wood and the mulberry twig in the subtropical regions as raw materials, can fully utilize the dominant resources in the subtropical regions, and reduces the cultivation cost of the ganoderma sinense;
3. inoculating the third-level seeds to the cut-log to prepare fungus sticks, and planting the fungus sticks in the clotted tea-oil tree inter-plot to enable the fungus sticks to naturally grow in the oil-oil tree inter-plot, so that on one hand, the wild-simulated planting of wild ganoderma sinense is facilitated, and the planted ganoderma sinense is rich in nutrient accumulation and high in nutritional value; on the other hand, the fungus sticks are planted in the closed tea-oil tree woodland, the wild-simulated planting adjustment of the ganoderma sinense is suitable, the planting of the ganoderma sinense is not required to be covered and watered, the planting cost is low, the development of ganoderma sinense planting is facilitated, meanwhile, the tea-oil tree woodland can be fully utilized, the potential of the woodland is further excavated, and the income of forest farmers is conveniently increased.
Drawings
Fig. 1 is a flow chart in an embodiment of the present invention.
Detailed Description
The present invention is described in further detail below with reference to fig. 1.
Example (b): an ecological cultivation method of wild Ganoderma in Guangxi comprises the following steps:
s1, selecting and processing the culture medium, preparing a first-class culture medium by adopting fresh green roughwood leaves, fresh mako leaves, peptone, sugarcane juice, agar and water, preparing a second-class culture medium by adopting miscellaneous wood chips, bagasse and gypsum powder, and preparing a third-class culture medium by adopting mako wood chips, wheat bran, sugar and gypsum powder.
S2, strain preparation and strain inoculation, wherein the method comprises the steps of collecting wild ganoderma species growing in the native areas of Guangxi in subtropical regions, separating the collected wild ganoderma species to prepare strains, inoculating the strains into a first-class strain culture medium to culture to obtain first-class strains, inoculating the first-class strains into a second-class strain culture medium to culture to obtain second-class strains, and inoculating the second-class strains into a third-class strain culture medium to obtain third-class strains.
S3, preparing a bacterium rod, namely inoculating the third-level seeds in the step S2 to the sterilized log for culture, and preparing the bacterium rod.
S4, preparing ground cultivation compost, and mixing the green hillock, the mulberry branch, the wood dust, the bagasse and the gypsum powder in proportion to prepare the ground cultivation compost for planting.
S5, selecting a ganoderma lucidum planting field to plant, taking the camellia oleifera forest land with closed inter-plant as a planting field, punching a plurality of rows of planting holes in the space between two adjacent rows of camellia oleifera trees by using planting punching equipment, wherein the distance between each row is 50-60cm, then laying the ground planting compost prepared in the step S4 in the planting holes, flatly placing the fungus sticks prepared in the step S3 in the planting holes, covering fine soil on the surfaces of the fungus sticks, completing planting operation, and enabling the planted fungus sticks to naturally grow into ganoderma sinense.
The size specification of the planting hole in the step S5 is 25cm × 25cm × 20 cm.
The mixture ratio of the first-class seed culture medium in the step S1 is as follows: 10-20 g of fresh green roughhaired holly leaves, 10-20 g of fresh gelia japonica leaves, 20-25 g of peptone, 30-40 g of sugarcane juice, 20-25 g of agar and 800ml of water. During the process of preparing the first-class seed culture medium, the components are weighed according to the proportion, stirred and mixed evenly, and then the first-class seed culture medium is obtained after sterilization treatment.
The weight ratio of the ingredients in the secondary strain culture medium in the step S1 is as follows: 60% of wood dust, 40% of bagasse and 1.5% of gypsum powder. The sawdust comprises the following components in parts by weight: 50% of green-hillock sawdust and 50% of mulberry branch sawdust. In the process of preparing the second-class culture medium, the components are weighed according to the proportion, stirred and mixed evenly, and then the second-class culture medium is obtained after sterilization treatment
The weight ratio of the ingredients in the third-class culture medium in the step S1 is as follows: 78% of wood chips, 10% of wheat bran, 1% of sugar and 1% of gypsum powder. In the process of preparing the three-level seed culture medium, the components are weighed according to the proportion, stirred and mixed uniformly, and then the three-level seed culture medium is obtained after sterilization treatment
The ground cultivation compost in the step S4 comprises the following ingredients in percentage by weight: 60% of green hillock wood, mulberry twig and bakelite sawdust, 38% of bagasse and 2% of gypsum powder. The green hillock, the mulberry branch, the bakelite wood dust and the bagasse are used as the ground culture material, and the waste bagasse is recycled, so that the resources are fully utilized.
After the planting operation is completed in the step S5, the camellia oleifera forest land is mowed and nurtured for 1 time in 2-3 months per year, and the ganoderma lucidum growth nutrient solution is periodically supplemented to the planting land, so that the ganoderma sinense can grow conveniently.
The working principle is as follows: the ecological cultivation method of the wild purple lucid ganoderma adopts wild lucid ganoderma varieties growing in the native place of Guangxi in subtropical regions, and finishes the cultivation process of strains in three levels by respectively preparing a first-level seed culture medium, a second-level seed culture medium and a third-level seed culture medium after separating the collected wild lucid ganoderma, so that the problems of single tree seeds and unreasonable cultivation material ingredients when a cultivation material is directly adopted by the strains can be avoided in the cultivation process, sufficient nutrient substances can be accumulated, and the lucid ganoderma with excellent quality can be conveniently cultivated. The advantageous resources of Guangxi in the subtropical regions, such as the bakelite, the green roughhaired holly and the mulberry twig, are used as raw material substitutes, so that the advantageous resources of Guangxi in the subtropical regions can be fully utilized, and the cultivation cost of the ganoderma sinense is reduced. And inoculating the third-level seeds to the cut-log to prepare a fungus stick, and planting the fungus stick in the intervarietal between the closed tea-oil trees to ensure that the fungus stick naturally grows in the intervarietal between the tea-oil trees, thereby facilitating the wild-simulated planting of the wild ganoderma sinense on the one hand, and ensuring that the planted ganoderma sinense has rich nutrient accumulation and high nutritional value. On the other hand, the fungus sticks are planted in the closed tea-oil tree woodland, the wild-simulated planting adjustment of the ganoderma sinense is suitable, the planting of the ganoderma sinense is not required to be covered and watered, the planting cost is low, the development of ganoderma sinense planting is facilitated, meanwhile, the tea-oil tree woodland can be fully utilized, the potential of the woodland is further excavated, and the income of forest farmers is conveniently increased.
The present embodiment is only for explaining the present invention, and it is not limited to the present invention, and those skilled in the art can make modifications of the present embodiment without inventive contribution as needed after reading the present specification, but all of them are protected by patent law within the scope of the claims of the present invention.
Claims (7)
1. An ecological cultivation method of Guangxi indigenous wild purple lucid ganoderma is characterized in that: the method specifically comprises the following steps:
s1, selecting and processing culture medium, preparing a first-class culture medium by adopting fresh green roughwood leaves, fresh mako leaves, peptone, sugarcane juice, agar and water, preparing a second-class culture medium by adopting miscellaneous wood chips, bagasse and gypsum powder, and preparing a third-class culture medium by adopting mako wood chips, wheat bran, sugar and gypsum powder;
s2, strain preparation and strain inoculation, wherein the method comprises the steps of collecting wild ganoderma lucidum varieties growing in subtropical regions, separating the collected wild ganoderma lucidum varieties to prepare strains, inoculating the strains into a first-class strain culture medium for culture to obtain first-class strains, inoculating the first-class strains into a second-class strain culture medium for culture to obtain second-class strains, and inoculating the second-class strains into a third-class strain culture medium to obtain third-class strains;
s3, preparing a bacterium rod, namely inoculating the third-level seeds in the step S2 to the sterilized log for culture to prepare the bacterium rod;
s4, preparing ground cultivation compost, mixing the green hillock, the mulberry branch, the wood dust, the bagasse and the gypsum powder in proportion to prepare the ground cultivation compost for planting;
s5, selecting a ganoderma lucidum planting field to plant, taking the camellia oleifera forest land with closed inter-plant as a planting field, punching a plurality of rows of planting holes in the space between two adjacent rows of camellia oleifera trees by using planting punching equipment, wherein the distance between each row is 50-60cm, then laying the ground planting compost prepared in the step S4 in the planting holes, flatly placing the fungus sticks prepared in the step S3 in the planting holes, covering fine soil on the surfaces of the fungus sticks, completing planting operation, and enabling the planted fungus sticks to naturally grow into ganoderma sinense.
2. The ecological cultivation method of Guangxi indigenous wild purple lucid ganoderma as claimed in claim 1, which is characterized in that: the size specification of the planting hole in the step S5 is 25cm × 25cm × 20 cm.
3. The ecological cultivation method of Guangxi indigenous wild purple lucid ganoderma as claimed in claim 1, which is characterized in that: the mixture ratio of the first-class seed culture medium in the step S1 is as follows: 10-20 g of fresh green roughhaired holly leaves, 10-20 g of fresh gelia japonica leaves, 20-25 g of peptone, 30-40 g of sugarcane juice, 20-25 g of agar and 800ml of water.
4. The ecological cultivation method of Guangxi indigenous wild purple lucid ganoderma as claimed in claim 1, which is characterized in that: the weight ratio of the ingredients in the secondary strain culture medium in the step S1 is as follows: 60% of wood dust, 40% of bagasse and 1.5% of gypsum powder; the sawdust comprises the following components in parts by weight: 50% of green-hillock sawdust and 50% of mulberry branch sawdust.
5. The ecological cultivation method of Guangxi indigenous wild purple lucid ganoderma as claimed in claim 1, which is characterized in that: the weight ratio of the ingredients in the third-class culture medium in the step S1 is as follows: 78% of wood chips, 10% of wheat bran, 1% of sugar and 1% of gypsum powder.
6. The ecological cultivation method of Guangxi indigenous wild purple lucid ganoderma as claimed in claim 1, which is characterized in that: the ground cultivation compost in the step S4 comprises the following ingredients in percentage by weight: 60% of green hillock wood, mulberry twig and bakelite sawdust, 38% of bagasse and 2% of gypsum powder.
7. The ecological cultivation method of Guangxi indigenous wild purple lucid ganoderma as claimed in claim 1, which is characterized in that: after the planting operation is completed in the step S5, the camellia oleifera forest land is mowed and nurtured for 1 time in 2-3 months per year, and the planting land is periodically supplemented with the lucid ganoderma growth nutrient solution.
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