CN111929438A - 一种检测非洲猪瘟病毒抗体的量子点微球免疫层析试纸条及其应用 - Google Patents
一种检测非洲猪瘟病毒抗体的量子点微球免疫层析试纸条及其应用 Download PDFInfo
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- CN111929438A CN111929438A CN202010806738.2A CN202010806738A CN111929438A CN 111929438 A CN111929438 A CN 111929438A CN 202010806738 A CN202010806738 A CN 202010806738A CN 111929438 A CN111929438 A CN 111929438A
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Abstract
Description
标准效价 | 标准值(荧光强度值,au) | 测定值(荧光强度值,au) | 测定效价/标准效价 |
1:1000 | 28.47106 | 27.88265 | 0.9718 |
1:2000 | 25.49187 | 24.93687 | 0.9690 |
1:4000 | 22.51568 | 22.27697 | 0.9840 |
1:8000 | 19.53349 | 19.98643 | 1.0380 |
1:16000 | 16.55430 | 16.19564 | 0.9599 |
1:32000 | 13.57511 | 14.13659 | 1.0942 |
1:64000 | 10.59592 | 10.93778 | 1.1147 |
Claims (10)
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Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN112730830A (zh) * | 2021-01-27 | 2021-04-30 | 成都微瑞生物科技有限公司 | 一种非洲猪瘟抗原双孔快速检测卡及检测方法 |
Citations (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103048460A (zh) * | 2012-12-15 | 2013-04-17 | 武汉珈源生物医学工程有限公司 | 一种用量子点荧光免疫层析试纸条检测的方法 |
CN109781980A (zh) * | 2019-01-30 | 2019-05-21 | 河南中泽生物工程有限公司 | 非洲猪瘟病毒快速检测卡及其应用 |
CN110658339A (zh) * | 2019-12-02 | 2020-01-07 | 北京纳百生物科技有限公司 | 非洲猪瘟病毒的检测试纸、试剂盒及其制备方法 |
CN110760006A (zh) * | 2019-10-31 | 2020-02-07 | 河南省生物工程技术研究中心 | 一种非洲猪瘟免疫***靶向基因工程疫苗 |
CN110873792A (zh) * | 2019-11-29 | 2020-03-10 | 江苏省农业科学院 | 非洲猪瘟病毒抗体检测试剂盒 |
CN210347664U (zh) * | 2019-04-29 | 2020-04-17 | 杭州艾替捷英科技有限公司 | 非洲猪瘟病毒量子点检测试纸条 |
CN210347666U (zh) * | 2019-04-30 | 2020-04-17 | 杭州艾替捷英科技有限公司 | 非洲猪瘟抗体胶体金检测卡 |
-
2020
- 2020-08-12 CN CN202010806738.2A patent/CN111929438B/zh active Active
Patent Citations (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103048460A (zh) * | 2012-12-15 | 2013-04-17 | 武汉珈源生物医学工程有限公司 | 一种用量子点荧光免疫层析试纸条检测的方法 |
CN109781980A (zh) * | 2019-01-30 | 2019-05-21 | 河南中泽生物工程有限公司 | 非洲猪瘟病毒快速检测卡及其应用 |
CN210347664U (zh) * | 2019-04-29 | 2020-04-17 | 杭州艾替捷英科技有限公司 | 非洲猪瘟病毒量子点检测试纸条 |
CN210347666U (zh) * | 2019-04-30 | 2020-04-17 | 杭州艾替捷英科技有限公司 | 非洲猪瘟抗体胶体金检测卡 |
CN110760006A (zh) * | 2019-10-31 | 2020-02-07 | 河南省生物工程技术研究中心 | 一种非洲猪瘟免疫***靶向基因工程疫苗 |
CN110873792A (zh) * | 2019-11-29 | 2020-03-10 | 江苏省农业科学院 | 非洲猪瘟病毒抗体检测试剂盒 |
CN110658339A (zh) * | 2019-12-02 | 2020-01-07 | 北京纳百生物科技有限公司 | 非洲猪瘟病毒的检测试纸、试剂盒及其制备方法 |
Non-Patent Citations (2)
Title |
---|
孙燕燕等: "基于金纳米颗粒标记非洲猪瘟病毒p30蛋白免疫层析检测方法的建立", 《中国兽医科学》 * |
江地科等: "检测猪瘟病毒胶体金和量子点试纸条的初步研制", 《江苏农业学报》 * |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN112730830A (zh) * | 2021-01-27 | 2021-04-30 | 成都微瑞生物科技有限公司 | 一种非洲猪瘟抗原双孔快速检测卡及检测方法 |
CN112730830B (zh) * | 2021-01-27 | 2023-12-29 | 杭州大瑞科技有限公司 | 一种非洲猪瘟抗原双孔快速检测卡及检测方法 |
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