CN111903673A - Diatomite and ryanodine receptor inhibitor insecticidal composition, preparation method thereof and application thereof in preventing and controlling tobacco stem borers - Google Patents
Diatomite and ryanodine receptor inhibitor insecticidal composition, preparation method thereof and application thereof in preventing and controlling tobacco stem borers Download PDFInfo
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- CN111903673A CN111903673A CN202010828948.1A CN202010828948A CN111903673A CN 111903673 A CN111903673 A CN 111903673A CN 202010828948 A CN202010828948 A CN 202010828948A CN 111903673 A CN111903673 A CN 111903673A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/08—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing solids as carriers or diluents
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N41/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a sulfur atom bound to a hetero atom
- A01N41/02—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a sulfur atom bound to a hetero atom containing a sulfur-to-oxygen double bond
- A01N41/10—Sulfones; Sulfoxides
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/48—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with two nitrogen atoms as the only ring hetero atoms
- A01N43/56—1,2-Diazoles; Hydrogenated 1,2-diazoles
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Abstract
The invention discloses a diatomite and ryanodine receptor inhibitor insecticidal composition, a preparation method thereof and application of the insecticidal composition in preventing and treating tobacco meal moth, wherein the insecticidal composition is formed by compounding components comprising diatomite and ryanodine receptor inhibitor. When in specific use, the insecticidal composition can be sprayed on the walls in the tobacco warehouse and the junctions of the supporting columns and the ground by a motor-driven powder sprayer to form an application tape with the width of 30-50 cm. The prevention and control results show that: the insecticidal composition for preventing and treating the tobacco meal borers, which is derived from diatomite and ryanodine receptor inhibitors, can effectively kill the tobacco meal borers, quickly reduce the population density of the tobacco meal borers in an initial flue-cured tobacco warehouse and reduce the loss of tobacco leaves.
Description
Technical Field
The invention relates to a diatomite and ryanodine receptor inhibitor insecticidal composition, a preparation method thereof and application thereof in preventing and controlling tobacco powder borers, belonging to the technical field of tobacco storage pest prevention and control.
Background
The tobacco pink borer (Ephestia elutella (Hubner)) belongs to the family Lepidoptera (Lepidotera) and Pimpinella punctifera (Phycididae), and is also called tobacco pink borer or tobacco stem borer. The tobacco powder borer is a parasitic storage pest, mainly harms tobacco leaves, can also eat wheat, soybean, pea, broad bean, peanut, cocoa bean, flour, various dried fruits and the like, and is one of two main pests in the tobacco leaf storage process.
The tobacco powder borer is widely distributed all over the world, and other provinces are reported in China except Tibet. In tobacco production in China, due to the fact that moisture content of initially-cured tobacco is high, climate conditions in south are proper, and sugar content of tobacco is high, the insects are more in south and southwest, such as Yunnan, Guizhou, Sichuan, Fujian, Jiangxi, Guangdong and Hunan, and the like, and the insects are more serious.
Although there are many effective methods for preventing and treating the tobacco powder borer in the tobacco industry in China, the application of some technologies is limited by the factors of warehouse conditions, the moisture content of tobacco leaves, logistics procedures and the like. In recent years, the occurrence of insect pest situation is aggravated due to the change of the supply and demand conditions of tobacco leaves and the prolonging of the storage time of the primary flue-cured tobacco leaves, and the occurrence of insect pest situation of the pink borer further causes trouble to the tobacco leaf sales of tobacco commercial enterprises, so that an effective method for rapidly reducing the insect pest population density of the pink borer in the primary flue-cured tobacco warehouse and reducing the loss probability of the tobacco leaves is needed to be researched.
Before pupating, the tobacco pink borer larva prefers to climb out of the gunny bag to the corner and the wall for pupation so as to find a mate for mating after eclosion. Therefore, the tobacco production practice can utilize the habit of the tobacco powder moth to control at the stage so as to reduce the quantity of imagoes capable of eclosion and greatly reduce the quantity of insect population of offspring.
Disclosure of Invention
Aiming at the problems in the prior art, the invention provides a diatomite and ryanodine receptor inhibitor insecticidal composition, a preparation method thereof and application thereof in preventing and controlling tobacco stem borer. The insecticidal composition has the advantages of low cost, easy operation, high efficiency, greenness and long lasting period, and can effectively kill the tobacco powder borer.
The invention relates to a diatomite and ryanodine receptor inhibitor insecticidal composition, which is formed by compounding components comprising diatomite and ryanodine receptor inhibitor.
The particle fineness of the diatomite is 120-1200 meshes, the adhesion of the ryanodine receptor inhibitor to the pores of the diatomite is facilitated, and meanwhile, the amorphous structure of the diatomite particles can pierce the internode membrane of the tobacco borer in motion, so that the body fluid loss of the diatomite is caused to death.
The ryanodine receptor inhibitor is selected from one or more of cyantraniliprole, flubendiamide and chlorantraniliprole.
The preparation method of the insecticidal composition of diatomite and ryanodine receptor inhibitors comprises the following steps:
step 1: evenly mixing diatomite and the ryanodine receptor inhibitor according to the weight ratio of 100-500:10 to obtain the master powder of the insecticidal composition; on one hand, the special porous structure of the diatomite is utilized to facilitate the full attachment of the ryanodine receptor inhibitor; on the other hand, the lasting period of the ryanodine receptor inhibitor is greatly prolonged.
Step 2: and (3) uniformly mixing the mother powder of the insecticidal composition obtained in the step (1) with diatomite according to the weight ratio of 1-2:10 to obtain the insecticidal composition of the diatomite and the ryanodine receptor inhibitor. The step of diluting the insecticidal composition is beneficial to the uniform dispersion of the ryanodine receptor inhibitor.
The invention relates to an application of a diatomite and ryanodine receptor inhibitor insecticidal composition, which is used for preventing and treating tobacco powder borer. The insecticidal composition can be sprayed on the walls in the tobacco warehouse and the junctions of the supporting columns and the ground by a motorized powder sprayer to form an application belt with the width of 30-50 cm.
Further, the insecticidal composition is sprayed on the wall in the tobacco warehouse and the ground at the junction of each support pillar and the ground to form an application belt with the width of 30-50 cm.
The larva of the tobacco powder borer climbs out of the gap of the tobacco wrapper and has the habit of pupation and eclosion on the wall of the warehouse and the gap. The invention utilizes the habit of spreading the composition of the diatomite and the ryanodine receptor on the wall edge and each supporting column edge of the warehouse, and when the larva climbs out, the larva is killed by adhering the composition of the diatomite and the ryanodine receptor. The insecticidal composition can effectively control the insect population density of the pink borer and reduce the loss of tobacco leaves.
Compared with the prior art, the invention has the beneficial effects that:
the raw material of the invention is diatomite formed by siliceous sedimentary rock of biological origin, which has the advantages of special porous structure, strong adsorption property, light volume weight, uniform fineness, neutral pH value, no toxicity, good mixing uniformity and the like, and has wide sources and low cost. According to the invention, the diatomite and the ryanodine receptor inhibitor are uniformly mixed, so that the ryanodine receptor inhibitor is attached to the pores of the diatomite, and the obtained insecticidal composition has the characteristics of high efficiency, greenness and long lasting period. Meanwhile, by utilizing the habit that the larvae of the tobacco meal moth climb out from gaps of tobacco wrapper and pupate and emerge on the wall of the warehouse and at the gaps, the diatomite and ryanodine receptor composition is spread on the edge of the wall of the warehouse and the edge of each supporting column, and when the larvae climb out, the larvae are adhered with the diatomite and ryanodine receptor composition to kill the larvae. The physical insecticidal function that the particles of the diatomite block air holes and pierce the body wall of the insect is exerted, so that the porous structure of the diatomite is fully utilized to adsorb the chemical agent and prolong the effective period of the chemical agent.
The test result shows that: the insecticidal composition containing the diatomite and the ryanodine receptor inhibitor is used for preventing and controlling the tobacco powder borer, and can effectively kill the tobacco powder borer and control the population density.
Drawings
Fig. 1 and 2 are graphs showing the distribution effect of the insecticidal composition on the edge of the wall around the tobacco warehouse.
Fig. 3 and 4 are graphs showing the killing effect of the insecticidal composition on tobacco stem borers.
FIG. 5 is a diagram showing the situation that the old larva of the pink borer climbs on the wall.
FIG. 6 is a sex attractant for attracting male imagoes of tobacco smoke dust borers, which is mainly used for monitoring insect situations.
FIG. 7 is comparative data of pesticidal effects in example 1.
Detailed Description
The present invention will be further described with reference to the following specific embodiments. It will be understood by those skilled in the art that these examples are for illustrative purposes only and are not intended to limit the scope of the present invention in any way.
The experimental procedures in the following examples are conventional unless otherwise specified. The raw materials and reagent materials used in the following examples are all commercially available products unless otherwise specified.
Example 1:
the influence of different mass ratios of the diatomite and the cyantraniliprole on the control effect is as follows: the time for storing the primary flue-cured tobacco leaves in the warehouse of the redrying plant is 3-6 months, the generation of the tobacco powder borers can be 2-3 generations, and the control effect and the sustainable control period of the primary insect source of the tobacco leaves in the warehouse have great influence on the insect situation and the appearance quality of the tobacco leaves during storage, so that the research and test are carried out on the control effect and the duration of the mixed powder of the diatomite and the cyantraniliprole on the tobacco powder borers in the primary flue-cured tobacco leaves warehouse.
The test is carried out in a certain redrying factory warehouse in the southeast tobacco district of China. The warehouse is a single-storey house warehouse, and the area of each single warehouse is 40m2And 4 storehouses are selected in the test.
3 kinds of mixed powder of the diatomite and the cyantraniliprole with the mass ratio are respectively prepared by the two dilution methods. Diatomite: cyantraniliprole is: m1, 100-500: 1; m2, 100-500: 10; m3, 100-500: 25. m1, M2 and M3 were then applied to the corners of the walls of the 3 storehouses, respectively, with a width of 15 cm. The other was used as a control. Selecting the wall and the corner opposite to the storehouse door as check points in each storehouse, selecting 1M in length on the original pesticide application powder belt for investigation, checking the death rate of the tobacco powder borers on the pesticide application powder belt, and simultaneously checking tobacco powder borer larvae distributed on the corresponding wall from 0-2M in height so as to determine the number of the surviving powder borers crossing the pesticide application belt; and (4) checking the number of the tobacco pink borers aged young insects on the wall surface of the corresponding position only by comparing with the storeroom, and checking once per week. Meanwhile, a tobacco powder moth sex trapper is arranged in each storehouse, and the quantity change of the tobacco powder moth imagoes in the storehouse is monitored. The total number of examinations was 12 times, which is approximately 3 months.
The data of the adult tobacco pink borers trapped from the 4 storehouses can clearly show that the curve trend of the adult tobacco pink borers trapped from the storehouses treated by the M1 is basically equivalent to that of the adult tobacco pink borers trapped from the storehouses treated by the M1, but the data value of the M1 is smaller than CK, which indicates that the M1 treatment has a certain inhibition effect on the tobacco pink borers and reduces the total population of the insects. The plots for M2 and M3 are also substantially identical, showing significant control at the first peak, followed by a substantially smooth line without the second peak of the population of the rotifer, compared to CK, and the treatments M2 and M3 showed longer control of the tobacco borer in the storehouse. Moreover, there was no difference between the two treatments. In practical application, the mass ratio of the diatomite to the cyantraniliprole can be 100-500:10, the formula is economical.
TABLE 1 influence of different mass ratios of diatomaceous earth to cyantraniliprole on tobacco meal moth
Example 2:
evenly mixing diatomite with the particle fineness of 120-1200 meshes and cyantraniliprole according to the weight ratio of 100-500:10 to prepare the mother powder of the insecticidal composition; mixing the mother powder with the particle finenessDiatomaceous earth of 120-1200 mesh in a ratio of 1-2:10 to obtain the insecticidal composition of the diatomite and the cyantraniliprole for preventing and controlling the tobacco meal moth; the diluted insecticidal composition is applied in a dosage of 150 g per bag, with a treatment area of 10X 0.5m per bag2And the pesticide is sprayed on the edge of the wall of the tobacco warehouse to perform a prevention and control test of the tobacco powder borer, and the pesticide is uniformly distributed on the ground as much as possible. The administration area was checked at 1m every 5 days after administration2The number of dead insects and the number of live insects of the larva of the tobacco meal moth which climbs out left and right. The mortality rate was calculated as dead number/(dead number + live number) × 100% using the following formula. After the examination of each experimental area (point) is finished, the worm is moved out for the second observation. The test started at 9/22/2016 and until 11/2016, the experimental area still had significant effects and was repeated twice. The specific results are shown in Table 2 below.
The experiment shows that the insecticidal composition using the algae soil and the ryanodine receptor inhibitor can effectively prevent and control the tobacco meal moth, the death rate of insect population in a treatment area is more than 75% within 2 months after treatment investigation, and the death rate of larvae of the two-point meal moth is still more than 90% until No. 11 month 23, and the insecticidal composition has the advantages of low cost, easy operation, high efficiency, greenness and long lasting period.
TABLE 2 investigation of the efficacy of insecticidal compositions of diatomaceous earth and cyantraniliprole against tobacco meal moth
Note: "ns" is the reason that the insects in the processing area are also removed by mistake when the reservoir area is sanitary, so that investigation cannot be carried out.
The above description of the specific embodiments of the present invention is not intended to limit the present invention, and those skilled in the art may make various changes and modifications according to the present invention without departing from the spirit of the present invention, which is defined by the scope of the appended claims.
Claims (9)
1. The insecticidal composition of diatomite and ryanodine receptor inhibitors is characterized in that:
the insecticidal composition is prepared by compounding ingredients including diatomite and a ryanodine receptor inhibitor.
2. The insecticidal composition of diatomaceous earth and a ryanodine receptor inhibitor of claim 1, wherein:
the particle fineness of the diatomite is 120-1200 meshes.
3. The insecticidal composition of diatomaceous earth and a ryanodine receptor inhibitor of claim 1, wherein:
the ryanodine receptor inhibitor is selected from one or more of cyantraniliprole, flubendiamide and chlorantraniliprole.
4. A process for the preparation of a pesticidal composition of diatomaceous earth and a ryanodine receptor inhibitor as claimed in claim 1, 2 or 3, comprising the steps of:
step 1: evenly mixing diatomite and a ryanodine receptor inhibitor to obtain mother powder of the insecticidal composition;
step 2: and (3) uniformly mixing the mother powder of the insecticidal composition obtained in the step (1) with diatomite again to obtain the insecticidal composition of the diatomite and the ryanodine receptor inhibitor.
5. The method of claim 4, wherein:
in the step 1, the mass ratio of the diatomite to the ryanodine receptor inhibitor is 100-500: 10.
6. The method of claim 4, wherein:
in the step 2, the mass ratio of the mother powder of the insecticidal composition to the diatomite is 1-2: 10.
7. The use of the insecticidal composition of diatomaceous earth and a ryanodine receptor inhibitor of claims 1, 2 or 3, wherein: the insecticidal composition is used for preventing and treating tobacco powder borers.
8. Use according to claim 7, characterized in that:
and (3) spraying the insecticidal composition on the wall in the tobacco warehouse and the junction between each support column and the ground by using a motorized powder sprayer to form an application tape with the width of 30-50 cm.
9. Use according to claim 8, characterized in that:
the insecticidal composition is sprayed on the wall in the tobacco warehouse and the ground at the junction of each support column and the ground to form an application band with the width of 30-50 cm.
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Application publication date: 20201110 |