CN111903431A - Jade fungus culture medium and preparation method thereof - Google Patents
Jade fungus culture medium and preparation method thereof Download PDFInfo
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- CN111903431A CN111903431A CN202010734073.9A CN202010734073A CN111903431A CN 111903431 A CN111903431 A CN 111903431A CN 202010734073 A CN202010734073 A CN 202010734073A CN 111903431 A CN111903431 A CN 111903431A
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- culture
- culture dish
- puddler
- micro motor
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/60—Cultivation rooms; Equipment therefor
- A01G18/64—Cultivation containers; Lids therefor
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F27/00—Mixers with rotary stirring devices in fixed receptacles; Kneaders
- B01F27/05—Stirrers
- B01F27/11—Stirrers characterised by the configuration of the stirrers
- B01F27/19—Stirrers with two or more mixing elements mounted in sequence on the same axis
- B01F27/191—Stirrers with two or more mixing elements mounted in sequence on the same axis with similar elements
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F27/00—Mixers with rotary stirring devices in fixed receptacles; Kneaders
- B01F27/60—Mixers with rotary stirring devices in fixed receptacles; Kneaders with stirrers rotating about a horizontal or inclined axis
- B01F27/70—Mixers with rotary stirring devices in fixed receptacles; Kneaders with stirrers rotating about a horizontal or inclined axis with paddles, blades or arms
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Mycology (AREA)
- Environmental Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention is suitable for the technical field of culture media, and provides a Jatropha curcas culture medium and a preparation method thereof, wherein the culture medium comprises a culture dish, a base, culture test paper, a micro motor, a stirring rod and a stirring blade, the base is fixedly connected to the bottom of the culture dish, the culture test paper is detachably connected to the inside of the culture dish, and the top of the micro motor is fixedly connected with the outer side wall of the culture dish; to cultivate the test paper tiling at the surface of nutrient solution, the side-mounting of culture dish has micro motor, fixedly connected with puddler on micro motor's the output, the puddler runs through the culture dish, and a plurality of stirring leaves of fixedly connected with on the puddler, with micro motor and external power source electric connection, it rotates to drive the puddler through micro motor, thereby it stirs the nutrient solution to drive the stirring leaf slow rotation, thereby the inside nutrient composition deposit of nutrient solution has been avoided, influence the growth and development of Auricularia fuscosa, delay the growth cycle's of Auricularia fuscosa problem.
Description
Technical Field
The invention belongs to the technical field of culture media, and particularly relates to a culture medium for a jatropha curcas and a preparation method thereof.
Background
The Auricularia fuscosuccinea is a new edible fungus variety bred by Li Jade academy team of Jilin agricultural university, named Auricularia fuscosuccinea or white Auricularia fuscosuccinea, commonly called bamboo ear in southern areas such as Fujian Zhejiang. The Auricularia fuscosuccinea has good quality in lesser Khingan area, Betula platyphylla area and Yichun area. The Auricularia fuscosuccinea has extremely high requirements on the production place environment, low yield, high price and rich nutrition, and is another precious edible fungus for replacing Auricularia fuscosuccinea.
At present, the process of cultivating the Auricularia aurlcula generally adopts a liquid culture medium to culture, the Auricularia aurlcula plants are convenient to grow and develop, the liquid culture medium is generally kept stand inside a greenhouse after inoculation, the internal nutrient components are easy to deposit after a long time, the growth and development of the Auricularia aurlcula are not facilitated, the growth period of the Auricularia aurlcula is prolonged, and the plant volume after growth is small.
Disclosure of Invention
The invention provides a Auricularia aurlcula culture medium and a preparation method thereof, aiming at solving the problems that the Auricularia aurlcula is cultured by adopting a liquid culture medium in the process of culturing Auricularia aurlcula at present, the growth and development of Auricularia aurlcula plants are facilitated, the liquid culture medium is generally kept still in a greenhouse after being inoculated, the deposition of internal nutritional ingredients is easy after a long time, the growth and development of Auricularia aurlcula are not facilitated, the growth period of Auricularia aurlcula is prolonged, and the volume of the grown.
The invention is realized in such a way that the culture medium for the euonymus japonicus comprises a culture dish, a base, culture test paper, a micro motor, a stirring rod and a stirring blade, wherein the base is fixedly connected to the bottom of the culture dish, the culture test paper is detachably connected to the inside of the culture dish, the top of the micro motor is fixedly connected with the outer side wall of the culture dish, the stirring rod is positioned on the output end of the micro motor and is fixedly connected with the output end of the micro motor, the stirring blade is positioned on the outer surface of the stirring rod and is fixedly connected with the stirring rod, and a proper amount of nutrient solution is contained in the culture dish.
Preferably, the culture dish is a round tubular structure, the bottom of the culture dish is sealed, and a round through hole is formed in the side wall of the culture dish.
Preferably, the base is a rectangular disc-shaped structure, the top of the base is fixedly connected with the bottom of the culture dish, and the diameter of the base is larger than that of the culture dish.
Preferably, the culture test paper is circular, the diameter of the culture test paper is equal to the inner diameter of the culture dish, and the culture test paper is clamped in the inner cavity of the culture dish.
Preferably, the puddler is cylindric structure, the puddler is located inside the culture dish, wherein one end of puddler is passed through circular through-hole runs through the culture dish, and with micro motor's output fixed connection.
Preferably, the number of the stirring blades is multiple, and the stirring blades are arranged around one end of the stirring rod, which is positioned in the culture dish, at equal intervals.
A preparation method of a culture medium for the agaricus bisporus comprises the following steps:
step 1, cooking plant raw materials, juicing and filtering;
step 2, adding a proper amount of saccharides, yeast powder, potassium dihydrogen phosphate and a proper amount of water into the filtered plant raw material juice;
step 3, filling the nutrient solution which is proportioned into a shake flask and shaking up at high speed;
step 4, introducing the uniformly shaken nutrient solution into a culture dish, and sterilizing at high temperature;
step 5, opening a top cover of the culture dish, and flatly spreading the culture test paper on the outer surface of the nutrient solution after sterilizing the culture test paper;
and 6, inoculating the strain onto the culture test paper by using an inoculating shovel under the aseptic condition.
Preferably, the plant raw materials comprise 0.5-1.5 wt% of bran, 1.5-2.5 wt% of corn, 1.5-2.5 wt% of soybean, 0.5-1.5 wt% of oat, 1.5-2.5 wt% of sugar, 1-2 wt% of yeast powder, 0.2 wt% of potassium dihydrogen phosphate and the balance of water.
Preferably, the time for shaking up the nutrient solution in a shake flask is not less than twenty minutes, and the time for sterilizing the nutrient solution at high temperature is not less than fifty minutes.
Compared with the prior art, the invention has the beneficial effects that: according to the invention, by arranging the culture dish, the culture test paper, the micro motor, the stirring rod and the stirring blades, when the culture dish is used, a proper amount of nutrient solution is added into the culture dish, then the culture test paper is tiled on the outer surface of the nutrient solution, the micro motor is installed on the side surface of the culture dish, the stirring rod is fixedly connected to the output end of the micro motor, the stirring rod penetrates through the culture dish, the stirring rod is fixedly connected with a plurality of stirring blades, the micro motor is electrically connected with an external power supply, and the stirring rod is driven to rotate through the rotation of the micro motor, so that the stirring blades are driven to slowly rotate to stir the nutrient solution, thereby avoiding the problems that the nutrient components in the nutrient solution are deposited, the growth and development of the Auricularia fuscosa are influenced, and the growth cycle of the Auricu.
Drawings
FIG. 1 is a schematic view of the overall structure of the present invention;
FIG. 2 is a schematic view of a stirring structure in the present invention;
FIG. 3 is a flow chart of the present invention.
In the figure: 11. a culture dish; 12. a base; 13. culturing test paper; 14. a micro motor; 15. a stirring rod; 16. stirring the leaves.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is described in further detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
Referring to fig. 1-2, the present invention provides a technical solution: the utility model provides a jade fungus culture medium, including culture dish 11, a pedestal 12, cultivate test paper 13, micro motor 14, puddler 15 and stirring leaf 16, base 12 fixed connection is in the bottom of culture dish 11, it connects inside 11 to cultivate test paper 13 and can dismantle, micro motor 14's top and culture dish 11 lateral wall fixed connection, puddler 15 is located micro motor 14's output, and with micro motor 14's output fixed connection, stirring leaf 16 is located the surface of puddler 15, and with puddler 15 fixed connection, 11 inside flourishing nutrient solutions that are equipped with of culture dish.
In this embodiment, circular through-hole has been seted up to culture dish 11 side, micro motor 14's top and culture dish 11's lateral wall fixed connection, fixedly connected with puddler 15 on micro motor 14's the output, the one end that micro motor 14 was kept away from to puddler 15 runs through culture dish 11 through circular through-hole, puddler 15 is located a plurality of stirring leaves of fixedly connected with 16 of serving of culture dish 11 inside, in the use, with micro motor 14 and external power source electric connection, rotate through micro motor 14 and drive puddler 15 and rotate, thereby drive and rotate with puddler 15 fixed connection's stirring leaf 16, carry out slow stirring to the nutrient solution of culture dish 11 inside, avoid the inside nutrient composition of nutrient solution to precipitate, influence the growth of beautiful fungus and the problem of development.
Further, the culture dish 11 is a circular tubular structure, the bottom of the culture dish 11 is sealed, and a circular through hole is formed in the side wall of the culture dish 11.
In this embodiment, set up culture dish 11 and be used for holding nutrient solution, set up circular through-hole in culture dish 11 side, make puddler 15 can run through inside getting into culture dish 11, stir the nutrient solution of culture dish inside, avoid the nutrient composition of nutrient solution inside to precipitate.
Further, the base 12 is a rectangular disc-shaped structure, the top of the base 12 is fixedly connected with the bottom of the culture dish 11, and the diameter of the base 12 is larger than that of the culture dish 11.
In this embodiment, a disk-shaped base 12 is attached to the bottom of the culture dish 11 to lower the center of gravity of the entire culture medium and to increase the contact area between the culture base part and the ground, thereby preventing the culture dish 11 from turning sideways.
Further, the culture test paper 13 is circular, and the diameter of the culture test paper 13 is equal to the inner diameter of the culture dish 11 and is clamped in the inner cavity of the culture dish 11.
In this embodiment, the culture test paper 13 is circular, and the diameter of the culture test paper 13 is equal to the inner diameter of the culture dish 11, so that the culture test paper 13 can be just clamped inside the culture dish 11 and float on the outer surface of the nutrient solution.
Further, puddler 15 is cylindric structure, and puddler 15 is located inside culture dish 11, and wherein one end of puddler 15 runs through culture dish 11 through circular through-hole to with micro motor 14's output fixed connection.
In this embodiment, the stirring rod 15 is disposed inside the culture dish 11, and the stirring rod 15 is fixedly connected to the output end of the micro motor 14, so as to drive the stirring rod 15 to rotate through the micro motor 14, thereby stirring the nutrient solution inside the culture dish 11.
Further, the number of the stirring blades 16 is plural, and the stirring blades 16 are arranged around the end of the stirring rod 15 inside the culture dish 11 at equal intervals.
In this embodiment, set up a plurality of stirring leaf 16 on puddler 15 for drive stirring leaf 16 through puddler 15 and rotate, with the inside sedimentary nutrient substance stirring of culture dish 11, avoid the nutrient substance deposit, influence the growth of beautiful auricularia auriculajudae, prolong the growth cycle's of beautiful auricularia auriculajudae problem.
Referring to fig. 3, a preparation method of a culture medium for agaricus bisporus comprises the following steps:
step 1, cooking plant raw materials, juicing and filtering;
step 2, adding a proper amount of saccharides, yeast powder, potassium dihydrogen phosphate and a proper amount of water into the filtered plant raw material juice;
step 3, filling the nutrient solution which is proportioned into a shake flask and shaking up at high speed;
step 4, introducing the uniformly shaken nutrient solution into a culture dish, and sterilizing at high temperature;
step 5, opening a top cover of the culture dish, and flatly spreading the culture test paper on the outer surface of the nutrient solution after sterilizing the culture test paper;
and 6, inoculating the strain onto the culture test paper by using an inoculating shovel under the aseptic condition.
Furthermore, the plant raw materials comprise 0.5-1.5 wt% of bran, 1.5-2.5 wt% of corn, 1.5-2.5 wt% of soybean, 0.5-1.5 wt% of oat, 1.5-2.5 wt% of sugar, 1-2 wt% of yeast powder, 0.2 wt% of potassium dihydrogen phosphate and the balance of water.
In the embodiment, the natural plant is mainly used, is natural, green and free of chemical residues, has a plurality of trace elements and comprehensive nutrition, is added with sugar to provide sufficient nutrition for the Auricularia aurlcula, and is added with monopotassium phosphate to provide phosphate fertilizer and potash fertilizer for the Auricularia aurlcula.
Furthermore, the nutrient solution is filled into a shake flask and shaken uniformly for not less than twenty minutes, and the high-temperature sterilization time of the nutrient solution is not less than fifty minutes.
In the embodiment, the nutrient solution is shaken for a long time to uniformly shake nutrient components in the nutrient solution, and the sterilization time is used for completely killing bacteria in the nutrient solution to avoid the bacteria from influencing the growth of plants.
In the embodiment, bran, corn, soybean and oat are cooked, and are put into a juicer for juicing, after juicing is finished, plant raw materials are filtered, 0.5-1.5% of bran, 1.5-2.5% of corn, 1.5-2.5% of soybean, 0.5-1.5% of oat, 1.5-2.5% of sugar, 1-2% of yeast powder, 0.2% of potassium dihydrogen phosphate and the balance of water are added into filtrate according to the weight ratio, nutrient solution preparation is carried out, after the preparation, the nutrient solution is put into a shake flask to be uniformly shaken at a high speed, the shaking time is not less than twenty minutes, then the uniformly shaken nutrient solution is led into a culture dish 11, the culture dish 11 and the nutrient solution inside are disinfected at a high temperature, the disinfection time is not less than fifty minutes, after the disinfection, the culture test paper 13 is spread on the outer surface of the nutrient solution inside the culture dish 11, then the culture test paper is planted on the culture test paper under the aseptic condition, the bacterial grows through absorbing the nutrient composition of 11 inside of culture dishes, circular through-hole has been seted up to 11 sides of culture dishes, micro motor 14's top and culture dishes 11's lateral wall fixed connection, fixedly connected with puddler 15 on micro motor 14's the output, the one end that micro motor 14 was kept away from to puddler 15 runs through culture dishes 11 through circular through-hole, puddler 15 is located one of 11 inside of culture dishes and serves a plurality of stirring leaves of fixedly connected with 16, during the use, with micro motor 14 and external power source electric connection, rotate through micro motor 14 and drive puddler 15 and rotate, thereby drive and puddler 15 fixed connection's stirring leaf 16 rotates, carry out slow stirring to the nutrient solution of 11 inside of culture dishes, avoid the inside nutrient composition of nutrient solution to precipitate, influence the growth of edible tree fungus jade and the problem of development.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents and improvements made within the spirit and principle of the present invention are intended to be included within the scope of the present invention.
Claims (9)
1. The utility model provides a yu mu ear culture medium, includes culture dish (11), base (12), cultivates test paper (13), micro motor (14), puddler (15) and stirring leaf (16), its characterized in that: base (12) fixed connection in the bottom of culture dish (11), culture test paper (13) can dismantle connect in inside culture dish (11), the top of micro motor (14) with the lateral wall fixed connection of culture dish (11), puddler (15) are located on the output of micro motor (14), and with the output fixed connection of micro motor (14), stirring leaf (16) are located the surface of puddler (15), and with puddler (15) fixed connection, culture dish (11) inside flourishing is equipped with the nutrient solution.
2. The culture medium of Pimenta dioica according to claim 1, wherein: the culture dish (11) is a circular tubular structure, the bottom of the culture dish (11) is sealed, and a circular through hole is formed in the side wall of the culture dish (11).
3. The culture medium of Pimenta dioica according to claim 1, wherein: the base (12) is of a rectangular disc-shaped structure, the top of the base (12) is fixedly connected with the bottom of the culture dish (11), and the diameter of the base (12) is larger than that of the culture dish (11).
4. The culture medium of Pimenta dioica according to claim 1, wherein: the culture test paper (13) is circular, the diameter of the culture test paper (13) is equal to the inner diameter of the culture dish (11), and the culture test paper is clamped in the inner cavity of the culture dish (11).
5. The culture medium of Pimenta dioica according to claim 2, wherein: puddler (15) are cylindric structure, puddler (15) are located inside culture dish (11), wherein one end of puddler (15) is passed through circular through-hole runs through culture dish (11), and with micro motor's (14) output fixed connection.
6. The culture medium of Pimenta dioica according to claim 1, wherein: the quantity of stirring leaf (16) is a plurality of, and is a plurality of stirring leaf (16) encircle in puddler (15) are located the inside one end equidistance of culture dish (11) is arranged.
7. A preparation method of a culture medium for the agaricus bisporus is characterized by comprising the following steps: the method comprises the following steps:
step 1, cooking plant raw materials, juicing and filtering;
step 2, adding sugar, yeast powder, potassium dihydrogen phosphate and water into the filtered plant raw material juice;
step 3, filling the nutrient solution which is proportioned into a shake flask and shaking up at high speed;
step 4, introducing the uniformly shaken nutrient solution into a culture dish, and sterilizing at high temperature;
step 5, opening a top cover of the culture dish, and flatly spreading the culture test paper on the outer surface of the nutrient solution after sterilizing the culture test paper;
and 6, inoculating the strain onto the culture test paper by using an inoculating shovel under the aseptic condition.
8. The method for preparing the culture medium of Pilus jaborandi according to claim 7, wherein: the plant raw materials comprise, by weight, 0.5-1.5% of bran, 1.5-2.5% of corn, 1.5-2.5% of soybean, 0.5-1.5% of oat, 1.5-2.5% of sugar, 1-2% of yeast powder, 0.2% of monopotassium phosphate and the balance of water.
9. The method for preparing the culture medium of Pilus jaborandi according to claim 7, wherein the time for shaking up the nutrient solution in the shake flask is not less than twenty minutes, and the time for sterilizing the nutrient solution at high temperature is not less than fifty minutes.
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| CN202010734073.9A CN111903431A (en) | 2020-07-27 | 2020-07-27 | Jade fungus culture medium and preparation method thereof |
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| CN205035375U (en) * | 2015-08-27 | 2016-02-17 | 东北林业大学 | A accuse temperature magnetic stirring device for domestic fungus liquid is cultivateed |
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| CN106544267A (en) * | 2016-12-06 | 2017-03-29 | 安徽睿知信信息科技有限公司 | A kind of microbial cultivation device |
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| CN110495324A (en) * | 2019-08-22 | 2019-11-26 | 安徽吉玲农业科技有限公司 | A kind of rice shoot culture apparatus |
| CN209816137U (en) * | 2019-03-12 | 2019-12-20 | 合肥职业技术学院 | Small fungus culture device |
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| US5049505A (en) * | 1988-09-27 | 1991-09-17 | Kabushiki Kaisha Komatsu Seisakusho | Breeding apparatus |
| CN102786334A (en) * | 2012-07-27 | 2012-11-21 | 龙源海洋生物股份有限公司 | Culture medium for culturing edible fungus production mother seeds |
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Application publication date: 20201110 |