CN111888526A - Skin sheet for mixed transplantation of heterogeneous species - Google Patents

Skin sheet for mixed transplantation of heterogeneous species Download PDF

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CN111888526A
CN111888526A CN202010545661.8A CN202010545661A CN111888526A CN 111888526 A CN111888526 A CN 111888526A CN 202010545661 A CN202010545661 A CN 202010545661A CN 111888526 A CN111888526 A CN 111888526A
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skin
sheet
pigskin
autologous
transplantation
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陈翔
何志友
魏天虹
赵爽
粟娟
魏红江
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/3604—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix characterised by the human or animal origin of the biological material, e.g. hair, fascia, fish scales, silk, shellac, pericardium, pleura, renal tissue, amniotic membrane, parenchymal tissue, fetal tissue, muscle tissue, fat tissue, enamel
    • A61L27/362—Skin, e.g. dermal papillae
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/3683—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix subjected to a specific treatment prior to implantation, e.g. decellularising, demineralising, grinding, cellular disruption/non-collagenous protein removal, anti-calcification, crosslinking, supercritical fluid extraction, enzyme treatment
    • A61L27/3695—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix subjected to a specific treatment prior to implantation, e.g. decellularising, demineralising, grinding, cellular disruption/non-collagenous protein removal, anti-calcification, crosslinking, supercritical fluid extraction, enzyme treatment characterised by the function or physical properties of the final product, where no specific conditions are defined to achieve this
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
    • A61L27/60—Materials for use in artificial skin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2430/00—Materials or treatment for tissue regeneration
    • A61L2430/40—Preparation and treatment of biological tissue for implantation, e.g. decellularisation, cross-linking

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Public Health (AREA)
  • Epidemiology (AREA)
  • Veterinary Medicine (AREA)
  • General Health & Medical Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Dermatology (AREA)
  • Medicinal Chemistry (AREA)
  • Oral & Maxillofacial Surgery (AREA)
  • Transplantation (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Botany (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Zoology (AREA)
  • Materials For Medical Uses (AREA)

Abstract

A skin sheet for xenogeneic mixed transplantation comprises a pigskin sheet and an autologous skin sheet, and is prepared by the following method: (1) preparing pigskin sheets; (2) preparing autologous skin sheets; the invention takes the pig as a donor, the donor has wide sources, and is processed into a special shape, so that the invention is convenient for embedding, mixing, transplanting and repairing the wound surface with the autologous skin of the patient at the later stage, can greatly relieve the current situation of skin source deficiency of the patient with large-area skin defects such as burns, skin avulsions and the like, reduces the supply area damage, saves the life of the patient, has great social value, has very large industrialization and commercialization values, and brings considerable economic value after being put into the clinical market.

Description

Skin sheet for mixed transplantation of heterogeneous species
Technical Field
The invention relates to a preparation method of a medical treatment biomaterial, in particular to a skin sheet for xenogeneic mixed transplantation.
Background
The large-area skin defects of patients, such as burns and skin avulsion, the lack of autologous skin sources and the difficulty in wound surface sealing are main causes of death of the patients. The acellular xenogenesis or xenogeneic pericarp, the artificial synthesized dermal matrix and the like which are widely applied clinically can play a role in temporarily protecting the wound surface or providing a bracket, but cannot replace autologous skin transplantation, and have the problem of poor anti-infection capability.
The cell-containing xenotransplantation with biological activity is the research focus at present, because pigs are easy to breed and are very similar to human beings in physiological and metabolic aspects, the possibility of the occurrence of the co-morbid diseases of the human pigs is low, ethical disputes are few, and the matching of donor tissues and organs can be enhanced through artificial gene modification, so the pigs are donors which are more suitable for the skin xenotransplantation. However, the traditional method for preparing and transplanting the pigskin sheet cannot completely avoid the occurrence of immune rejection and is difficult to fundamentally repair the wound surface for a long time, so that no practical application exists in clinic.
CN201510025968.4 discloses a preparation method of dressing skin, which comprises the following steps: dehairing the xenoskin raw material, and scraping fat and cuticle to obtain a xenoskin material with the thickness of 0.3-0.5 mm; soaking the xenogenic leather in NaCl solution, washing with sterile water, soaking in neutral protease solution with the mass fraction of 0.2-0.5% at 37 ℃, and washing with sterile water to obtain xenogenic leather; and inactivating the xenoskin to obtain dressing skin, wherein the inactivation comprises the following steps: heating the xenoskin at 50-60 ℃ for 60-120 minutes, and irradiating the xenoskin with gamma rays. The prepared dressing skin has no biological activity after inactivation treatment, can temporarily protect the wound surface, but is difficult to fundamentally repair the wound surface for a long time, and has poor anti-infection capability.
Disclosure of Invention
The invention aims to solve the technical problem of overcoming the defects of the prior art and provide a pigskin sheet for xenotransplantation, which can overcome the defect of difficult wound repair and even death of a patient with large-area skin defect due to the defect of skin sources of the patient.
The technical scheme for solving the technical problems is that the skin sheet for xenogeneic mixed transplantation comprises a pigskin sheet and an autologous skin sheet, and is prepared by the following method:
(1) preparing pigskin sheets: selecting fresh pigskin suitable for xenotransplantation, performing aseptic operation, cutting a complete large pigskin sheet with the thickness of 0.1-1mm from the fresh pigskin by using a skin-taking knife, cleaning the sheet by using normal saline, and cutting the sheet into square blocks with the side length of a cm in a mechanical cutting or laser cutting mode, wherein a is more than or equal to 0.2 and less than or equal to 2;
(2) preparing an autologous skin sheet: cutting the autologous skin with the thickness of 0.1mm-1mm cut by the dermatome into square blocks with the side length of b cm by a mechanical cutting or laser cutting mode, wherein b is more than or equal to 0.2 and less than or equal to 2.
A skin sheet for xenogeneic mixed transplantation comprises a pigskin sheet and an autologous skin sheet, and is prepared by the following method:
(1) preparing pigskin sheets: selecting fresh pigskin suitable for xenotransplantation, performing aseptic operation, cutting a complete large pigskin sheet with the thickness of 0.1-1mm by using a dermatome, cleaning the large pigskin sheet by using normal saline, and opening small square holes with the side length of d cm in the sheet at a fixed interval of c cm in a mechanical cutting or laser cutting mode, wherein c is more than or equal to 0.2 and less than or equal to 2, and d is more than or equal to 0.2 and less than or equal to 2;
(2) preparing an autologous skin sheet: cutting the autologous skin with the thickness of 0.1mm-1mm cut by the dermatome into square small blocks with the side length of e cm by a mechanical cutting or laser cutting mode, wherein e = d.
Further, the numerical values of a and b in the step (2) can be adjusted, the transplantation ratio of the pigskin sheet to the autologous skin can be adjusted, and the transplantation ratio is as follows: b2/(a2+b2)。
Further, the values of c and d in the steps (2) and (3) can be adjusted, the transplantation ratio of the pigskin sheet to the autologous skin can be adjusted, and the transplantation ratio is as follows: d2/(c+d)2。
Further, the pigskin sheet prepared in the step (1) is rinsed with normal saline and stored in a low-temperature humid environment for clinical use.
The invention also protects the application of the pigskin sheet for xenotransplantation in preparing products for xenotransplantation.
The invention also provides a product for the pigskin sheet for the xenotransplantation, which comprises the prepared graft skin sheet.
The invention also protects the simple deformation of the cut pigskin slices on the basis of the cutting mode of the invention, and is suitable for the cutting mode of xenogeneic mixed transplantation.
On the basis of a large number of experiments and verifications, the invention adopts the pig as a donor, is used for mixed transplantation and repair of the wound surface through a specific processing and preparation method, can effectively reduce the occurrence of immune rejection, and can provide a large number of stable donor sources for clinic.
Compared with the prior art, the invention has the following advantages: 1) the source of the pig as a skin donor is wide, and at present, more fresh pig skin subjected to gene modification is used as the skin donor, so that the effect is better; 2) the skin sheet is processed into a form suitable for xenogeneic mixed transplantation, the xenogeneic and autologous skin mixed transplantation can reduce the occurrence of immune rejection, and the skin sheet can survive and effectively protect the wound surface after transplantation; 3) by adopting heterogeneous mixed transplantation, patients do not need to take anti-immune rejection medicines additionally, autologous skin can grow and expand along with the lapse of time, heterogeneous skin is completely replaced finally, and the wound surface is not exposed in the process.
The invention takes fresh pigskin as a skin donor to prepare the skin sheet for xenotransplantation consisting of the pig epidermis and a small amount of dermal tissue, can permanently repair the wound surface at one time, greatly reduces the using amount of autologous skin, can effectively relieve the current situation that the wound surface of a patient is difficult to repair and even die due to the shortage of autologous skin of a patient with large-area skin defect at present, greatly improves the cure rate of large-area skin defect, reduces the supply area damage, and has great medical value and economic value.
Drawings
FIG. 1 is a schematic view of a skin sheet for xenogeneic transplantation according to example 1 of the present invention;
FIG. 2 is a schematic view of a skin patch for xenotransplantation according to example 2 of the present invention.
In the figure: 1. pigskin sheet, 11. inlay hole, 2. autologous skin sheet, 3. wound surface.
Detailed Description
The following examples are given to facilitate a better understanding of the invention, but do not limit the invention. The experimental procedures in the following examples are conventional unless otherwise specified.
Example 1
The embodiment comprises the following steps:
(1) preparation of pigskin sheet 1: selecting fresh pigskin suitable for xenotransplantation (the fresh pigskin refers to pigskin with bioactivity), performing aseptic operation, and cutting a complete large sheet of pigskin with the thickness of 0.1-1mm with a skin-taking knife to obtain a sheet containing the components of pig epidermis and a small amount of dermis and having bioactivity; cleaning the complete large pig skin sheet with normal saline, and then cutting the whole large pig skin sheet into square blocks with side length of a cm in a mechanical cutting or laser cutting mode, wherein a is more than or equal to 0.2 and less than or equal to 2; in order to keep the skin piece stretched, a supporting material such as vaseline gauze can be covered on one side of the cuticle of the skin piece in advance; the biological activity means that cell components in the skin sheet exist and can still survive after transplantation;
(2) preparing an autologous skin sheet 2: cutting autologous skin with the thickness of 0.1mm-1mm cut by a skin-taking knife into square blocks with the side length of b cm by a mechanical cutting or laser cutting mode, wherein b is more than or equal to 0.2 and less than or equal to 2; the size of the autologous skin sheet 2 can be adjusted according to the actual situation, and the use ratio of the autologous skin sheet 2 to the pigskin sheet 1 during transplantation is adjusted;
the detailed preparation steps are as follows:
Figure DEST_PATH_IMAGE001
preparing a skin supply area: the relative hidden positions such as head, back, belly, thigh etc. are generally selected to supply the skin district, if supply the skin district limited, for repairing the surface of a wound as early as possible, save the life, then any undamaged skin all can regard as to supply the skin district. Removing hair from skin, sterilizing, and injecting into skinIrradiating 1/200000-concentration epinephrine normal saline solution, and optionally lidocaine for anesthesia;
Figure 116329DEST_PATH_IMAGE002
cutting: the cutting of the skin sheet is carried out by adopting a skin taking knife or a skin drum, autologous skin with the thickness of 0.1mm-1mm can be directly cut, and for parts which are easy to sew, such as the abdomen and the like, the whole layer of the skin can be directly cut and then processed into the thickness of 0.1mm-1mm for later use;
Figure DEST_PATH_IMAGE003
treating the skin supply area after skin taking: for the skin supply area directly cut into 0.1mm-1mm skin pieces, dressing change is carried out periodically after the wound surface is bound for hemostasis, and after the wound surface is naturally healed, the skin supply area of the whole layer of skin is cut and cut, and then the skin supply area is bound after being sewed;
Figure 33470DEST_PATH_IMAGE004
cutting the leather sheet: after obtaining the self-body leather piece, directly adhering the leather piece or the surface of the skin with a support material such as vaseline gauze, and cutting the leather piece into small squares with the size of b cm for use by adopting a mechanical cutting or laser cutting mode.
(3) Wound surface 3 debridement: thoroughly debriding the operation aspect to be subjected to mixed transplantation, and ensuring no necrotic infected tissue, no active hemorrhage, no obvious bone exposure and no large vessel nerve exposure; the granulation of the wound surface is fresh, and the higher survival rate after transplantation can be ensured.
(4) Preparation and transplantation of skin sheets for mixed xenotransplantation (pigskin sheet 1 and autologous skin sheet 2): the pigskin sheet 1 and the autologous skin sheet 2 prepared in the steps (2) and (3) are mutually crossed, embedded and transplanted on the wound surface 3, the dermis surface faces the wound surface, the epidermis layer faces outwards, and the transplantation of the xenogeneic species is realized through cutting, splicing and other modes; the pigskin sheet 1 and the autologous pigskin sheet 2 are matched with the size and shape of the wound surface, cover all the wound surface range, and are properly fixed by adopting a suture, as shown in figure 1;
(5) wrapping: after the mixed transplantation of the pigskin sheet 1 and the autologous pigskin sheet 2 is completed, the pigskin sheet 1 and the autologous pigskin sheet 2 are properly wrapped and maintained with certain pressure, so that the transplanted pigskin sheet 1 and the autologous pigskin sheet 2 are tightly attached to the wound surface and cannot move or float. The bandaging mode can adopt common sterile gauze pressurization or packing bandaging, and can also adopt some special products to pressurize and bandaging.
Rinsing the pigskin sheet 1 prepared in the step (1) with normal saline, and storing the pigskin sheet in a low-temperature humid environment for clinical use.
The numerical values of a and b in the steps (1) and (2) are in the range of 0.2-2cm, the use ratio of the xenogeneic pericarp to the autologous pericarp, namely the transplantation ratio, can be adjusted according to the situation by adjusting the actual sizes of a and b, and the transplantation ratio is as follows: b2/(a2+b2). Under the condition of special scarcity of the autologous skin, the transplantation ratio (the use ratio of the autologous skin) can be reduced to 0.01, and the utilization efficiency of the autologous skin is greatly improved.
Example 2
The embodiment comprises the following steps:
(1) preparation of pigskin sheet 1: selecting fresh pigskin suitable for xenotransplantation (the fresh pigskin refers to pigskin with bioactivity), performing aseptic operation, and cutting a complete large sheet of pigskin with the thickness of about 0.1-1mm by using a skin-taking knife, wherein the obtained sheet contains the components of pig epidermis and a small amount of dermis and has bioactivity; after the integral large pig skin piece is cleaned by normal saline, square small holes (embedding holes 11) with the side length of d cm are formed in the large pig skin piece in a mechanical cutting or laser cutting mode according to a fixed distance of c cm, wherein c is more than or equal to 0.2 and less than or equal to 2, d is more than or equal to 0.2 and less than or equal to 2, and in order to keep the skin piece unfolded, one side of the cuticle of the skin piece can be covered with support materials such as vaseline gauze in advance; the biological activity means that cell components in the skin sheet exist and can still survive after transplantation;
(2) preparing an autologous skin sheet 2: cutting the autologous skin with the thickness of 0.1mm-1mm cut by the dermatome into small blocks with the size of e cm square in a mechanical cutting or laser cutting mode, wherein e = d. The size of the autologous pigskin sheet 2 is matched with the size of the embedding hole 11 formed in the pigskin sheet 1, and the use ratio of the autologous pigskin sheet 2 to the pigskin sheet 1 during transplantation can be adjusted by changing the size of the embedding hole 11 of the pigskin sheet 1;
the detailed preparation steps are as follows:
Figure 626256DEST_PATH_IMAGE001
preparing a skin supply area: the relative hidden positions such as head, back, belly, thigh etc. are generally selected to supply the skin district, if supply the skin district limited, for repairing the surface of a wound as early as possible, save the life, then any undamaged skin all can regard as to supply the skin district. Before taking skin, removing hair from skin, sterilizing, subcutaneously injecting 1/200000-concentration epinephrine normal saline solution, and optionally adding lidocaine for anesthesia;
Figure 21466DEST_PATH_IMAGE002
cutting: the cutting of the skin sheet is carried out by adopting a skin taking knife or a skin drum, autologous skin with the thickness of 0.1mm-1mm can be directly cut, and for parts which are easy to sew, such as the abdomen and the like, the whole layer of the skin can be directly cut and then processed into the thickness of 0.1mm-1mm for later use;
Figure 835838DEST_PATH_IMAGE003
treating the skin supply area after skin taking: for the skin supply area directly cut into 0.1mm-1mm skin pieces, dressing change is carried out periodically after the wound surface is bound for hemostasis, and after the wound surface is naturally healed, the skin supply area of the whole layer of skin is cut and cut, and then the skin supply area is bound after being sewed;
cutting the leather sheet: after obtaining the self-body leather piece, directly adhering the leather piece or the surface of the skin with a support material such as vaseline gauze, and cutting the leather piece into small squares with the size of d cm for use by adopting a mechanical cutting or laser cutting mode.
(3) Wound surface 3 debridement: thoroughly debriding the operation aspect to be subjected to mixed transplantation, and ensuring no necrotic infected tissue, no active hemorrhage, no obvious bone exposure and no large vessel nerve exposure; the granulation of the wound surface is fresh, and the higher survival rate after transplantation can be ensured.
(4) Preparation and transplantation of skin sheets for mixed xenotransplantation (pigskin sheet 1 and autologous skin sheet 2): the tape processed in the step (1) is processedThe pigskin sheet 1 with the self-skin embedded holes 11 (small square holes with the side length of d cm) has a dermis surface close to the wound surface and an epidermis surface facing outwards, is pasted on the wound surface, enables the size and the shape of the transplanted xenogeneic skin sheet to be equivalent to the wound surface 3 through cutting, splicing and other modes, covers all the wound surface 3 ranges, and is properly fixed by adopting stitches;
Figure 720617DEST_PATH_IMAGE002
inlaying the autologous skin sheet 2 prepared in the step (3) into an autologous skin inlaying hole 11 (namely a small square hole with the side length of d cm) on the pigskin sheet 1, wherein the dermis surface faces the wound surface, the epidermis layer faces outwards, and the dermis layer is inlaid and transplanted on the wound surface to realize the full coverage of the wound surface, as shown in the attached drawing 2;
(6) wrapping: after the mixed transplantation of the pigskin sheet 1 and the autologous pigskin sheet 2 is completed, the pigskin sheet 1 and the autologous pigskin sheet 2 are properly wrapped and maintained with certain pressure, so that the transplanted pigskin sheet 1 and the autologous pigskin sheet 2 are tightly attached to the wound surface and cannot move or float. The bandaging mode can adopt common sterile gauze pressurization or packing bandaging, and can also adopt some special products to pressurize and bandaging.
Rinsing the pigskin sheet 1 prepared in the step (1) with normal saline, and storing the pigskin sheet in a low-temperature humid environment for clinical use.
The numerical values of c and d in the steps (1) and (2) are 0.2-2cm, the use ratio of the xenogeneic pericarp to the autologous pericarp, namely the transplantation ratio, can be adjusted according to the conditions by adjusting the actual sizes of c and d, and the transplantation ratio is as follows: d2/(c+d)2. Under the condition of special scarcity of the autologous skin, the transplantation ratio (the use ratio of the autologous skin) can be reduced to 0.01, and the utilization efficiency of the autologous skin is greatly improved.
The implanted wound surface 3 is required to reach the skin grafting condition which is as follows: the wound is thoroughly debrided, no necrotic infected tissue, no active hemorrhage, no obvious bone exposure and large vessel nerve exposure, and the granulation of the wound surface is fresh. And the transplanted mixed skin sheets are properly bound and fixed.
The invention takes fresh pigskin as a skin donor to prepare the skin sheet for xenotransplantation consisting of the pig epidermis and a small amount of dermal tissue, can permanently repair the wound surface at one time, greatly reduces the using amount of autologous skin, can effectively relieve the current situation that the wound surface of a patient is difficult to repair and even die due to the shortage of autologous skin of a patient with large-area skin defect at present, greatly improves the cure rate of large-area skin defect, reduces the supply area damage, and has great medical value and economic value.
Various modifications and variations of the present invention may be made by those skilled in the art, and they are still within the scope of the present patent invention provided they are within the scope of the claims and their equivalents.
What is not described in detail in the specification is prior art that is well known to those skilled in the art.

Claims (5)

1. The utility model provides a skin piece is used in xenogenesis mixed transplantation, includes pigskin skin piece and autologous skin piece, its characterized in that: the preparation method comprises the following steps:
(1) preparing pigskin sheets: selecting fresh pigskin suitable for xenotransplantation, performing aseptic operation, cutting a complete large pigskin sheet with the thickness of 0.1-1mm from the fresh pigskin by using a skin-taking knife, cleaning the sheet by using normal saline, and cutting the sheet into square blocks with the side length of a cm in a mechanical cutting or laser cutting mode, wherein a is more than or equal to 0.2 and less than or equal to 2;
(2) preparing an autologous skin sheet: cutting the autologous skin with the thickness of 0.1mm-1mm cut by the dermatome into square blocks with the side length of b cm by a mechanical cutting or laser cutting mode, wherein b is more than or equal to 0.2 and less than or equal to 2.
2. The utility model provides a skin piece is used in xenogenesis mixed transplantation, includes pigskin skin piece and autologous skin piece, its characterized in that: the preparation method comprises the following steps:
(1) preparing pigskin sheets: selecting fresh pigskin suitable for xenotransplantation, performing aseptic operation, cutting a complete large pigskin sheet with the thickness of 0.1-1mm by using a dermatome, cleaning the large pigskin sheet by using normal saline, and opening small square holes with the side length of d cm in the sheet at a fixed interval of c cm in a mechanical cutting or laser cutting mode, wherein c is more than or equal to 0.2 and less than or equal to 2, and d is more than or equal to 0.2 and less than or equal to 2;
(2) preparing an autologous skin sheet: cutting the autologous skin with the thickness of 0.1mm-1mm cut by the dermatome into square small blocks with the side length of e cm by a mechanical cutting or laser cutting mode, wherein e = d.
3. The skin sheet for xenotransplantation according to claim 1, which comprises: adjusting the values of a and b in the step (2), and adjusting the transplantation ratio of the pigskin sheet to the autologous skin, wherein the transplantation ratio is as follows: b2/(a2+b2)。
4. The skin sheet for xenotransplantation according to claim 2, wherein: adjusting the values of c and d in the steps (2) and (3), and adjusting the transplantation ratio of the pigskin sheet to the autologous skin, wherein the transplantation ratio is as follows: d2/(c+d)2。
5. The pigskin sheet for xenotransplantation according to claim 1 or 2, wherein: rinsing the pigskin sheet prepared in the step (1) with normal saline, and storing the pigskin sheet in a low-temperature humid environment for clinical use.
CN202010545661.8A 2020-06-16 2020-06-16 Skin sheet for mixed transplantation of heterogeneous species Pending CN111888526A (en)

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Publication number Priority date Publication date Assignee Title
CN101874903A (en) * 2009-11-20 2010-11-03 胡庆柳 Method for preparing collagen artificial skin
WO2012174239A2 (en) * 2011-06-16 2012-12-20 Akron General Autologous in situ tissue engineering
CN105617461A (en) * 2016-01-27 2016-06-01 深圳爱生再生医学科技有限公司 Skin patch for treating skin burn and preparation method of skin patch
CN110876635A (en) * 2019-12-20 2020-03-13 武汉维斯第医用科技股份有限公司 Skin grafting tool and method for manufacturing composite skin sheet

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101874903A (en) * 2009-11-20 2010-11-03 胡庆柳 Method for preparing collagen artificial skin
WO2012174239A2 (en) * 2011-06-16 2012-12-20 Akron General Autologous in situ tissue engineering
CN105617461A (en) * 2016-01-27 2016-06-01 深圳爱生再生医学科技有限公司 Skin patch for treating skin burn and preparation method of skin patch
CN110876635A (en) * 2019-12-20 2020-03-13 武汉维斯第医用科技股份有限公司 Skin grafting tool and method for manufacturing composite skin sheet

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