CN111855361A - Dip dyeing type automatic dyeing device and using method - Google Patents

Dip dyeing type automatic dyeing device and using method Download PDF

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Publication number
CN111855361A
CN111855361A CN202010705697.8A CN202010705697A CN111855361A CN 111855361 A CN111855361 A CN 111855361A CN 202010705697 A CN202010705697 A CN 202010705697A CN 111855361 A CN111855361 A CN 111855361A
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China
Prior art keywords
reagent
dyeing
slide
dip
axis motor
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CN202010705697.8A
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Chinese (zh)
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印进寨
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Shanghai Weijinshi Biotechnology Co Ltd
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Shanghai Weijinshi Biotechnology Co Ltd
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Priority to CN202010705697.8A priority Critical patent/CN111855361A/en
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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/28Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • G01N1/31Apparatus therefor

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Sampling And Sample Adjustment (AREA)

Abstract

The invention provides a dip-dyeing type automatic dyeing device and a using method thereof, and relates to the technical field of dyeing instrument equipment. The reagent cover driving motor ensures that the reagent cover can be automatically opened and closed without manual intervention, so that the reagent cover is simpler and more convenient to open and close; the weighing sensor at the bottom of the reagent box enables workers to know the amount of the residual reagent in the reagent box, and if the reagent in the reagent box is insufficient, the workers can be reminded to add the reagent in time; the draining fan can make the stained or cleaned slide rack have less residual liquid and be cleaner.

Description

Dip dyeing type automatic dyeing device and using method
Technical Field
The invention relates to the technical field of dyeing instrument equipment, in particular to a dip-dyeing type automatic dyeing device and a using method thereof.
Background
With the development of the technology, an automatic dyeing device is gradually adopted, so that the dyeing is standardized, the inspection result is controllable, and the existing biological and medical sample dyeing equipment mainly comprises three types of spray dyeing, injection dyeing and dip dyeing. The spray dyeing is realized by atomizing the dyeing liquid through an atomizing nozzle and then spraying the atomized dyeing liquid on the glass slide to dye the sample; the injection dyeing is to drip a reagent on the slide by a pump to achieve the purpose of dyeing; the dip dyeing is that a slide carrying a sample is placed in a dye vat filled with a dyeing solution to realize the dyeing of the sample. The spray dyeing is poor in dyeing effect, and meanwhile, as the atomizing nozzle is small in hole, the instrument is inevitably blocked during actual crystallization, so that the fault rate is high, the maintenance is complex, and the instrument is gradually eliminated; the injection dyeing has large reagent dosage, which leads to cost increase; by adopting the dip dyeing mode, the slide is immersed into the reagent, the dyeing effect completely avoids the defects of spray dyeing and injection dyeing.
At present, the following problems exist when the dyeing is carried out by adopting a dip dyeing mode: (1) a reagent cover for sealing the reagent box needs to be opened and closed manually; (2) the amount of the reagents left in the reagent box cannot be known, and when the amount of the reagents in the reagent box is insufficient, workers cannot be reminded of adding the reagents in time, so that the dyeing work is influenced; (3) after staining or after the slide rack is cleaned, a small amount of reagents or clear water may remain. Therefore, the above problems become problems to be solved urgently by those skilled in the art.
Disclosure of Invention
The invention aims to solve the technical problem of overcoming the defects in the prior art and provides a dip-dyeing type automatic dyeing device and a using method thereof.
The invention is realized by the following technical scheme: the utility model provides an automatic dyeing apparatus of dip-dyeing formula, a serial communication port, including the dyeing apparatus body, the dyeing apparatus body includes the casing, is located the moving mechanism of casing, and dyeing mechanism washs the pond, stoving mechanism and draining device, be equipped with bracket and reagent lid driving motor in the casing, the bracket links to each other with moving mechanism, dyeing mechanism includes the kit and inserts the slide frame on the bracket, the bottom fixed mounting of kit has weighing sensor, and top surface fixed mounting has the spout and covers with spout sliding connection's reagent, reagent lid driving motor fixed mounting is in the casing rear end and is connected with the reagent lid, be equipped with water inlet and delivery port in the washing pond, stoving mechanism includes drying tank and heating device, draining device sets up the top at the bracket.
According to the above technical solution, preferably, the moving mechanism includes an X-axis motor for controlling the carriage to move laterally and a Z-axis motor disposed at the top end of the carriage for controlling the slide rack to move up and down.
According to the technical scheme, preferably, the shell is internally and fixedly provided with the mounting plate, and the mounting plate is connected with the bracket in a sliding mode.
According to the technical scheme, preferably, a filter is arranged at the water inlet.
According to the technical scheme, preferably, the outer surface of the shell is provided with the touch screen.
According to the technical scheme, preferably, a liquid level sensor is arranged above the cleaning pool.
According to the above technical scheme, preferably, the drying tank is provided with a heating device, and a heating sheet is arranged in the heating device.
According to the above technical solution, preferably, a soft corrosion-resistant material is attached to the lower bottom surface of the reagent cover, and the soft corrosion-resistant material is a rubber pad.
According to the above technical solution, preferably, the draining device is a fan.
The use method of the dip dyeing type automatic dyeing device is characterized by comprising the following steps:
s1: turning on the device, placing the slide on the slide rack, and then inserting the slide rack into the carrier;
s2: moving: controlling an X-axis motor to drive the slide rack to move to the upper part of the kit by using a touch screen;
s3: dip dyeing: the reagent cover driving motor is controlled to open the reagent cover, the slide frame is controlled by the Z-axis motor to reciprocate up and down in the reagent box to fully dye the slide, and after the dyeing is completed, the reagent cover driving motor is controlled again to close the reagent cover, so that the sealing performance of the reagent box is ensured, the volatilization of the reagent is prevented, and the possibility of pollution is reduced to the maximum extent.
S4: and moving the dyed slide rack to a position below the draining fan by the X-axis motor, and starting the draining fan to accelerate the removal of the residual reagent on the slide.
S5: the washing water in the washing tank firstly passes through the water inlet and then enters the washing tank through the filter, and water is discharged after the water overflows the water outlet through continuous water inlet, so that the purpose of water circulation is achieved on the one hand, and the washing effect of the washing tank is also guaranteed on the other hand.
S6: cleaning: the X-axis motor controls the slide frame to be above the cleaning pool, and the Z-axis motor controls the slide frame to enter the cleaning pool for cleaning.
S7: drying: the X-axis motor and the Z-axis motor control the slide frame to enter the drying groove, and residual liquid on the slide is removed under the combined action of the draining fan and the heating assembly.
According to the above technical solution, preferably, the number of slide glass placements in S1 is 12, and the touch screen in S2 is used for setting the dip dyeing time, cleaning time, and drying time of each reagent, and the sequence of reagent dip dyeing, slide glass cleaning, and slide glass drying.
The invention has the beneficial effects that: the reagent cover driving motor ensures that the reagent cover can be automatically opened and closed without manual intervention, so that the reagent cover is simpler and more convenient to open and close; the weighing sensor at the bottom of the reagent box enables workers to know the amount of the residual reagent in the reagent box, and if the reagent in the reagent box is insufficient, the workers can be reminded to add the reagent in time; the draining fan can make the stained or cleaned slide rack have less residual liquid and be cleaner.
Drawings
Fig. 1 shows a schematic structural diagram of an embodiment of the present invention.
Fig. 2 shows a schematic view of a backside structure of an embodiment of the present invention.
FIG. 3 is a schematic diagram showing the structure of a cleaning tank according to an embodiment of the present invention.
In the figure: 1. a housing; 2. a cleaning tank; 3. mounting a plate; 4. a bracket; 5. a kit; 6. a slide rack; 7. a weighing sensor; 8. a chute; 9. a reagent cover; 10. a water inlet; 11. a water outlet; 12. a drying tank; 13. a draining fan; 14. an X-axis motor; 15. a Z-axis motor; 16. a reagent cover drive motor; 17. a filter; 18. a touch screen; 19. a liquid level sensor; 20. a heating assembly; 21. and (7) a rubber pad.
Detailed Description
In order to make the technical solutions of the present invention better understood by those skilled in the art, the present invention will be further described in detail with reference to the accompanying drawings and preferred embodiments.
As shown in the figure, the invention provides an automatic dip dyeing device which is characterized by comprising a dyeing device body, the dyeing device body comprises a shell 1, a moving mechanism, a dyeing mechanism, a cleaning pool 2 and a drying mechanism which are positioned in the shell 1, a bracket 4 and a reagent cover driving motor 16 are arranged in the shell 1, the bracket 4 is connected with a moving mechanism, the dyeing mechanism comprises a reagent box 5 and a slide rack 6 inserted on the bracket 4, the bottom end of the reagent box 5 is fixedly provided with a weighing sensor 7, the top surface is fixedly provided with a chute 8 and a reagent cover 9 connected with the chute 8 in a sliding way, the reagent cover driving motor 16 is fixedly arranged at the rear end of the shell 1 and is connected with the reagent cover 9, a water inlet 10 and a water outlet 11 are arranged in the cleaning pool 2, the drying mechanism comprises a drying groove 12 positioned between the reagent kit 5 and the cleaning pool 2 and a draining fan 13 fixedly arranged at the top end of the bracket 4.
When dyeing is carried out, the reagent cover driving motor drives the reagent cover 9 to be opened, the moving mechanism drives the slide frame 6 on the bracket 4 to enter the reagent box 5 for dyeing, after dyeing is finished, the moving mechanism moves the slide frame 6 into the cleaning pool 2, the reagent cover driving motor 16 closes the reagent cover 9, the opening and closing work of the reagent cover 9 can be finished without manual intervention, labor is saved, the automation degree of the device is higher, in addition, compared with manual operation, the opening and closing of the reagent cover 9 is controlled by using the reagent cover driving motor 16, the sealing effect of the reagent cover 9 is ensured, the volatilization of a reagent is reduced to the maximum degree, and the possibility of reagent pollution is reduced to the maximum degree; cleaning water in the cleaning pool 2 firstly passes through the water inlet and then enters the cleaning pool 2 through the filter 17, and water is continuously fed, so that the water flows out after the water overflows the water outlet, on one hand, the purpose of water circulation is achieved, on the other hand, the cleaning effect of the cleaning pool 2 is also ensured, after the cleaning is finished, the slide frame 6 is moved to the drying groove 12 by the moving mechanism, under the combined action of the drying groove 12 and the draining fan 13, the quick drying of the slide is realized, in addition, the draining fan can blow the slide after the slide comes out of the reagent, so that the residual liquid is less and cleaner; the weighing sensor 7 arranged below the reagent kit 5 can acquire the volume of the reagent by acquiring the whole weight of the reagent kit 5, and can remind a worker to add the reagent in time when the reagent amount is insufficient.
According to the above embodiment, preferably, the moving mechanism includes the X-axis motor 14 for controlling the lateral movement of the carriage and the Z-axis motor 15 provided at the top end of the carriage for controlling the up-and-down movement of the slide rack. Thereby drive slide frame 6 through the horizontal migration of control X axle motor 14 control bracket to move to 5 tops of kit, utilize the circulation about the slide frame 6 of Z axle motor 15 control to move, dye, can make the contact of slide and reagent more abundant, improve the dyeing effect.
According to the above embodiment, preferably, the housing 1 is internally and fixedly provided with the mounting plate 3, and the mounting plate 3 is slidably connected with the bracket 4. The mounting plate 3 may facilitate mounting of the moving mechanism with the dyeing mechanism.
According to the above embodiment, it is preferable that the filter 17 is provided at the water inlet 10 to improve the degree of cleanliness of water, thereby improving the cleaning effect of the slide.
According to the above embodiment, preferably, the outer surface of the housing 1 is provided with the touch screen 18, so that the staff can conveniently set the dip-dyeing time and the dyeing process of each reagent.
According to the above embodiment, preferably, a liquid level sensor 19 is arranged above the cleaning tank 2, when abnormal conditions such as the blocking of the water outlet of the cleaning tank occur, the liquid level sensor 19 senses that the liquid level exceeds the limit position, and then the system closes the water inlet valve to stop water inlet in time.
According to the above embodiment, it is preferable that the drying tub 12 is provided with the heating unit 20, and the lower bottom surface of the reagent cover 9 is attached with the soft corrosion-resistant material.
According to the above embodiment, preferably, the heating sheet is arranged in the heating assembly, the soft corrosion-resistant material is the rubber pad 21, the heating sheet in the heating assembly 20 improves the drying effect of the drying groove 12, and the rubber pad 21 ensures the sealing effect of the reagent cover 9.
The use method of the dip dyeing type automatic dyeing device is characterized by comprising the following steps:
s1: turning on the apparatus, placing the slide on the slide rack 6, and then inserting the slide rack 6 into the carrier 4;
s2: moving: the touch screen 18 is used for controlling the X-axis motor 14 to drive the slide rack 6 to move to the upper part of the reagent box 5;
s3: dip dyeing: the reagent cover driving motor 16 is controlled to open the reagent cover, the slide frame 6 is controlled to reciprocate up and down in the reagent box 5 through the Z-axis motor 15 to fully dye the slide, and after the dyeing is finished, the reagent cover driving motor 16 is controlled again to close the reagent cover, so that the sealing performance of the reagent box 5 is ensured, the volatilization of the reagent is prevented, and the possibility of pollution is reduced to the maximum extent.
S4: the X-axis motor 14 moves the stained slide rack 6 to a position below the draining fan 13, and the draining fan 13 is started to accelerate the removal of the residual reagent on the slide.
S5: wash the washing water in the pond 2 and pass through water inlet 10 earlier, get into in wasing pond 2 through filter 17 again, through continuous intaking, after water overflows delivery port 11, the log raft is discharged, has reached hydrologic cycle's purpose on the one hand, and on the other hand has also guaranteed the cleaning performance who washs pond 2.
S6: cleaning: the X-axis motor 14 controls the slide frame 6 to be above the cleaning pool 2, and the Z-axis motor 15 controls the slide frame 6 to enter the cleaning pool 2 for cleaning.
S7: drying: the X-axis motor 14 and the Z-axis motor 15 control the slide frame 6 to enter the drying groove 12, and residual liquid on the slide is removed under the combined action of the draining fan 13 and the heating assembly.
According to the above embodiment, preferably, the number of slide glass placements in S1 is 12, and the touch screen 18 in S2 is used to set the dip-dyeing time, the washing time, and the drying time of each reagent, and the order of the reagent dip-dyeing, the slide glass washing, and the slide glass drying.
The invention has the beneficial effects that: the reagent cover driving motor 16 ensures that the reagent cover 9 can be automatically opened and closed without manual intervention, so that the reagent cover is simpler and more convenient to open and close; the weighing sensor 7 at the bottom of the reagent box 5 enables a worker to know the amount of the residual reagent in the reagent box 5, and if the reagent in the reagent box 5 is insufficient, the worker is reminded to add the reagent in time; the drain fan 13 may leave less liquid and be cleaner on the slide racks 6 after staining or washing.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.

Claims (10)

1. The utility model provides an automatic dyeing apparatus of dip-dyeing formula, a serial communication port, including the dyeing apparatus body, the dyeing apparatus body includes the casing, is located the moving mechanism of casing, and dyeing mechanism washs the pond, stoving mechanism and draining device, be equipped with bracket and reagent lid driving motor in the casing, the bracket links to each other with moving mechanism, dyeing mechanism includes the kit and inserts the slide frame on the bracket, the bottom fixed mounting of kit has weighing sensor, and top surface fixed mounting has the spout and covers with spout sliding connection's reagent, reagent lid driving motor fixed mounting is in the casing rear end and is connected with the reagent lid, be equipped with water inlet and delivery port in the washing pond, stoving mechanism includes drying tank and heating device, draining device sets up the top at the bracket.
2. The dip dyeing type automatic dyeing device according to claim 1, wherein the moving mechanism comprises an X-axis motor for controlling the lateral movement of the carriage and a Z-axis motor disposed at the top end of the carriage for controlling the up-and-down movement of the slide rack.
3. The automatic dip dyeing device according to claim 1, wherein a mounting plate is fixedly arranged in the housing, and a bracket is slidably connected to the mounting plate.
4. The dip dyeing type automatic dyeing apparatus according to claim 1, wherein a filter is provided at said water inlet.
5. The dip dyeing type automatic dyeing apparatus according to claim 1, wherein a touch screen is provided on an outer surface of said housing.
6. The dip dyeing type automatic dyeing apparatus according to claim 1, wherein a liquid level sensor is provided above said washing tank.
7. The dip dyeing type automatic dyeing device according to claim 1, characterized in that a heating device is arranged on the drying tank, a heating plate is arranged in the heating device, a soft corrosion-resistant material is attached to the lower bottom surface of the reagent cover, and the soft corrosion-resistant material is a rubber pad.
8. The draining device of claim 1 being a fan.
9. The use method of the dip dyeing type automatic dyeing device is characterized by comprising the following steps:
s1: turning on the device, placing the slide on the slide rack, and then inserting the slide rack into the carrier;
S2: moving: controlling an X-axis motor to drive the slide rack to move to the upper part of the kit by using a touch screen;
s3: dip dyeing: the reagent cover driving motor is controlled to open the reagent cover, the slide frame is controlled by the Z-axis motor to reciprocate up and down in the reagent box to fully dye the slide, and after the dyeing is completed, the reagent cover driving motor is controlled again to close the reagent cover, so that the sealing performance of the reagent box is ensured, the volatilization of the reagent is prevented, and the possibility of pollution is reduced to the maximum extent.
S4: and moving the dyed slide rack to a position below the draining fan by the X-axis motor, and starting the draining fan to accelerate the removal of the residual reagent on the slide.
S5: the washing water in the washing tank firstly passes through the water inlet and then enters the washing tank through the filter, and water is discharged after the water overflows the water outlet through continuous water inlet, so that the purpose of water circulation is achieved on the one hand, and the washing effect of the washing tank is also guaranteed on the other hand.
S6: cleaning: the X-axis motor controls the slide frame to be above the cleaning pool, and the Z-axis motor controls the slide frame to enter the cleaning pool for cleaning.
S7: drying: the X-axis motor and the Z-axis motor control the slide frame to enter the drying groove, and residual liquid on the slide is removed under the combined action of the draining fan and the heating assembly.
10. The use method of the dip dyeing type automatic dyeing apparatus according to claim 9, characterized in that the number of slide placing in S1 is 12, and the touch screen in S2 is used to set the dip dyeing time, cleaning time and drying time of each reagent and the sequence of the reagent dip dyeing, slide cleaning and slide drying.
CN202010705697.8A 2020-07-21 2020-07-21 Dip dyeing type automatic dyeing device and using method Pending CN111855361A (en)

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Application Number Priority Date Filing Date Title
CN202010705697.8A CN111855361A (en) 2020-07-21 2020-07-21 Dip dyeing type automatic dyeing device and using method

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Application Number Priority Date Filing Date Title
CN202010705697.8A CN111855361A (en) 2020-07-21 2020-07-21 Dip dyeing type automatic dyeing device and using method

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Publication Number Publication Date
CN111855361A true CN111855361A (en) 2020-10-30

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CN202010705697.8A Pending CN111855361A (en) 2020-07-21 2020-07-21 Dip dyeing type automatic dyeing device and using method

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113702142A (en) * 2021-09-10 2021-11-26 杭州米太科技有限公司 Cell staining instrument

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113702142A (en) * 2021-09-10 2021-11-26 杭州米太科技有限公司 Cell staining instrument

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