CN111849825B - Vibrio cholerae antagonistic strain and application thereof - Google Patents
Vibrio cholerae antagonistic strain and application thereof Download PDFInfo
- Publication number
- CN111849825B CN111849825B CN202010747624.5A CN202010747624A CN111849825B CN 111849825 B CN111849825 B CN 111849825B CN 202010747624 A CN202010747624 A CN 202010747624A CN 111849825 B CN111849825 B CN 111849825B
- Authority
- CN
- China
- Prior art keywords
- strain
- vibrio cholerae
- antagonistic
- aquaculture
- vibrio
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/07—Bacillus
- C12R2001/125—Bacillus subtilis ; Hay bacillus; Grass bacillus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
- A01N63/22—Bacillus
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/66—Microorganisms or materials therefrom
- A61K35/74—Bacteria
- A61K35/741—Probiotics
- A61K35/742—Spore-forming bacteria, e.g. Bacillus coagulans, Bacillus subtilis, clostridium or Lactobacillus sporogenes
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
- A61P1/14—Prodigestives, e.g. acids, enzymes, appetite stimulants, antidyspeptics, tonics, antiflatulents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
- A61P37/02—Immunomodulators
- A61P37/04—Immunostimulants
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Microbiology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Medicinal Chemistry (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Pharmacology & Pharmacy (AREA)
- Veterinary Medicine (AREA)
- Wood Science & Technology (AREA)
- General Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Immunology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Virology (AREA)
- Mycology (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Oncology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Dentistry (AREA)
- Environmental Sciences (AREA)
- Communicable Diseases (AREA)
- Nutrition Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Epidemiology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention belongs to the technical field of microorganisms, and provides a vibrio cholerae antagonistic strain and application thereof. The strain is Bacillus subtilis H6The mutant strain YJY20-01 has been preserved in China general microbiological culture Collection center (CGMCC No. 20013) in 6 months in 2020, and when in use, the bacterial agent prepared by the strain is applied to a culture pond to inhibit vibrio cholerae in water body and adjust the concentration of antagonistic bacteria in the culture water to 105cfu/ml, the inhibition rate of the invention strain on vibrio cholerae reaches 99.99% after being added for 48 hours, and the bacteriostasis and the stability are both superior to those of similar vibrio medicaments in the market.
Description
Technical Field
The invention belongs to the technical field of microorganisms, and provides a vibrio cholerae antagonistic strain and application thereof.
Background
Vibrio cholerae belongs to the class Proteobacteria, Vibrio (Vibrio), and is widely found in aquatic products such as rivers, seawater, fish, shrimp, shellfish, etc. Vibrio cholerae in aquaculture water is strong in vitality and low in nutritional requirement, and is easy to reproduce in aquaculture water, the Vibrio cholerae belongs to conditional pathogenic bacteria, once the water environment is deteriorated, the resistance of aquatic animals is weakened, the Vibrio cholerae can break down and cause damage to the animals, main manifestation symptoms comprise death, white feces, ulcer, slow growth and the like, and once outbreak occurs, a large number of aquatic animals can die and are difficult to control, and serious people can cause abortion. In addition, ingestion of water or aquatic products contaminated with pathogenic Vibrio cholerae can cause fatal dehydrated diarrhea and even cause fulminant epidemics of cholera disease.
At present, chemical treatment combining antibiotics and disinfectants is a main means for preventing and treating vibriosis in aquaculture industry, the excessive and intensive use of antibiotics and other chemical agents in the aquaculture process can increase the drug resistance and the drug resistance of pathogenic bacteria, simultaneously can cause pollution to aquaculture water and reduce the immunity of aquaculture organisms, and in addition, people can hurt human bodies by eating the aquatic products which are excessively polluted by the chemical agents.
In recent years, the research of probiotics in aquaculture is attracting more and more attention, and researches show that the effects of the probiotics in aquaculture mainly include competitive exclusion of pathogenic bacteria, provision of nutrients, promotion of growth of cultured animals, stimulation of immune system reaction, improvement of immunity, improvement of water quality conditions, restoration of ecological environment and the like. However, no effective strain and method for preventing and treating vibrio cholerae in aquaculture exist at present, and the problem needs to be solved urgently.
Disclosure of Invention
In order to solve the defects of the prior art, the invention provides a vibrio cholerae antagonistic strain, which is obtained by separating, screening and mutating antagonistic strains by taking common vibrio cholerae as an indicator in an aquaculture base, has strong inhibition on the vibrio cholerae and excellent probiotic effect, has excellent inhibition effect on vibrio pathogenic microorganisms caused in aquaculture, especially has more prominent effect on the vibrio cholerae, and has the inhibition rate of the vibrio cholerae in water bodies of up to 99.99 percent by applying the strain or a prepared microbial inoculum thereof to aquaculture pools, and does not influence the environment of the aquaculture water bodies, thereby reducing the risk of infecting the vibrio cholerae by aquatic animals.
The vibrio cholerae antagonistic strain is Bacillus subtilis (Bacillus subtilis) with the number YJY20-01, and is preserved in China general microbiological culture Collection center (CGMCC) 6 months in 2020 with the preservation number of CGMCC No. 20013; the 16SrDNA sequence is shown as Seq ID No. 1, and the sequence is the complete sequence of 16SrDNA of the strain;
the determined 16s rdna sequence has more than 99% homology with the nucleotide sequence of different strains of Bacillus (Bacillus sp.) and 100% homology with the strain in which the Bacillus subtilis is explicitly labeled, by BLAST alignment.
The strain is gram-positive bacteria, has no capsule and is rod-shaped; the bacterial colony on the ordinary nutrient agar plate is in a circular protrusion shape, and the bacterial colony is smooth, not rough, milky and opaque.
The sources of the vibrio cholerae antagonistic strain YJY20-01 are as follows:
the inventor separates a bacillus subtilis H with optimal antagonistic effect on vibrio cholerae, easy culture and stable passage characteristic from a plurality of shrimp ponds in an aquaculture base of Shandong Qiaozhuang6The strain is used as an initial strain to carry out ARTP mutagenesis, 135 single strains are obtained by separation, an optimal positive mutant strain YJY20-01 is obtained after antagonistic primary screening and co-culture secondary screening, and the growth rate of the mutagenic strain is higher than that of the initial strain in the culture process, so that the environmental adaptability is very good.
The strain can be applied to preventing and treating vibrio cholerae in aquaculture, has good preventing and treating effect on vibriosis caused by other vibrios, and can be directly put into aquaculture water body when in application, or can be put into aquaculture water body after being prepared into a microbial inoculum product through expanded culture.
Preferably, the initial density of the Vibrio cholerae antagonistic strain in the aquaculture water is 0.01-1 times, more preferably 0.01-0.1 times that of the Vibrio cholerae antagonistic strain. The density range can not only quickly achieve the effect of inhibiting the vibrio cholerae, but also save the cost. And the use method is simple, the dosage is less, and the operation is easy.
In conclusion, the bacillus subtilis YJY20-01 has obvious antagonism on vibrio cholerae, can obviously inhibit the propagation of the vibrio cholerae in a water body when being used for aquaculture, has an antagonism rate of 99.99% on the vibrio cholerae in the aquaculture water body within 48h, has persistence and stability superior to similar vibrio medicaments in the market, does not pollute the aquaculture water body, can promote the growth of cultured animals to a certain extent, stimulates the reaction of an immune system, improves the immunity, improves the water quality condition, repairs the ecological environment and the like.
Preservation information
Preservation time: 2020, 06 and 03 months
The name of the depository: china general microbiological culture Collection center
The preservation number is: CGMCC No.20013
The address of the depository: xilu No. 1 Hospital No. 3 of Beijing market facing Yang district
And (3) classification and naming: bacillus subtilis
Drawings
FIG. 1 shows the results of the plate antagonism experiment of example 1;
FIG. 2 is a graph showing the change in the amount of Vibrio cholerae bacteria in the ecological culture pond of example 2;
FIG. 3 is a graph showing the change in the amount of Vibrio cholerae bacteria in the outdoor culture pond of example 3.
Detailed Description
The present invention will be described in further detail with reference to the following examples, but it should not be construed that the scope of the present invention is limited to the following examples. All the technologies realized based on the above contents of the present invention belong to the scope of the present invention, and the following embodiments are all completed by adopting the conventional prior art except for the specific description.
The vibrio cholerae referred to in the following examples is all common vibrio cholerae in water in an aquaculture base of johan, eastern george.
EXAMPLE 1 characterization of the Strain YJY20-01
A. Plate antagonism experiment (antagonism prescreening):
the method comprises the following steps: inoculating activated Vibrio cholerae and antagonistic strain into fresh sterile LB liquid culture medium, culturing overnight, centrifuging the antagonistic strain culture medium respectively (4500rpm,5min), collecting thallus, and diluting to 10% with sterile water6cfu/ml；
Coating 100 mu l of vibrio cholerae in a fresh TCBS solid culture medium, punching 2 sterilized holes with the diameter of 10mm equidistantly on a flat plate coated with the vibrio cholerae by using an aseptic puncher, dripping 10 mu l of antagonistic strain diluent into the holes, culturing in an incubator at 35 ℃ overnight, observing whether a bacteriostatic ring appears around the holes or not about 24 hours, and measuring the diameter of the bacteriostatic ring, wherein transparent rings appear around the starting strain and the mutagenic strain as shown in figure 1, and the transparent rings of the mutagenic strain are larger.
The experimental data are shown in table 1:
TABLE 1 results of plate antagonism
Note: 0.05 ≤ P <0.1, 0.01 ≤ P ≤ 0.05, and P ≤ 0.01
B. Co-culture experiment (co-culture rescreening):
taking the activated starting strain H6The mutant strain YJY20-01 and the vibrio cholerae are respectively inoculated into an LB liquid culture medium to be cultured overnight, the culture solution is centrifuged (4500rpm,5min) to collect thalli, and then the thalli are respectively diluted by sterile water until the number of the thalli is 107cfu/ml；
Starting strain H6The mutant strain YJY20-01 is respectively and independently inoculated with vibrio cholerae into the same conical flask filled with 100ml of liquid LB culture medium diluted by 5 times for co-culture, a blank control (only vibrio cholerae is inoculated) is additionally arranged, and the inoculation amount of each strain is 200 mul of thallus dilutionCulturing at 37 deg.C and 150r/min for 24 hr.
The results of the experiment are shown in table 2: the inhibition rate of the mutant strain YJY20-01 on vibrio cholerae is as high as 99.99999 percent (almost 100 percent), which is far higher than that of the original strain H6And through the determination of the number of bacteria in the experimental process, the reproduction rate of YJY20-01 in the same vibrio cholerae environment is far higher than that of the starting strain H6, which shows that the environmental adaptability is extremely strong.
TABLE 2 results of the Co-cultivation experiments
Note: inhibition rate calculation methods (this method is used in all inhibition rate calculation methods in the present application):
x is the amount of vibrio cholerae bacteria (cfu/ml) after the blank control group is cultured;
y is the bacterial count (cfu/ml) of the antagonistic strain and the Vibrio cholerae after co-culture.
C. Passage stability experiment of mutagenic strain YJY20-01
The strain YJY20-01 is subjected to continuous 5-generation passage experiments to verify the genetic stability, and the results of the 5-generation strains after being co-cultured with vibrio cholerae respectively are shown in table 3, and the results show that the strains after being continuously passed for 5 generations have no obvious difference in the antagonistic effect on the vibrio cholerae and can stably inherit the high antagonistic performance to offspring (the experimental method is the same as the B co-culture experimental method).
TABLE 3YJY20-01 passage stability experiment results
D. Bench scale experiment
Taking a 500ml conical flask, adding 100ml water in an aquaculture pond into the conical flask, inoculating the centrifugal thallus (4500rpm,5min) of antagonistic bacteria according to the inoculation rate of 0.5% (V/V), taking the unopposed antagonistic bacteria as a blank control, performing shaking culture at 37 ℃ for 150r/min, and sampling every day to detect the change of the vibrio cholerae in the system.
Table 4 bench test results
The result is shown in Table 4, the experimental trial tracking is carried out for 3 days, the mutant strain YJY20-01 has a remarkable inhibiting effect on the vibrio cholerae in the system, and the inhibition on the vibrio cholerae in the system is not detected for 3 consecutive days after the addition, and reaches 100%.
EXAMPLE 2 use of the Strain YJY20-01
The volume of the culture water in a certain indoor culture pond is about 80m3The initial value of the cultured water is 1.55 x 106cfu/ml, antagonistic bacteria YJY20-01 and strain H6Respectively adding the fermented centrifugal thalli into a culture pond, and adjusting the concentration of antagonistic bacteria in the culture water to 105cfu/ml, taking a culture pond without the addition of antagonistic bacteria as a blank control, sampling every day to detect the content of vibrio cholerae in the water body, and carrying out an experiment for 5 days.
The results of the laboratory experiments are shown in FIG. 2, blank control and strain H6The number of the vibrio cholerae in the group water body is in an increasing trend along with the increase of experimental days, but the number of the vibrio cholerae in the starting strain group is slowly increased and does not increase by orders of magnitude, the amount of the vibrio cholerae in the system is always obviously lower than that of the other two groups after the fungicide is added into the mutagenic strain YJY20-01 group, and the vibrio cholerae cannot be basically detected in the YJY20-01 fungicide group system after 2 days of addition.
EXAMPLE 3 use of the Strain YJY20-01
The method is applied to an outdoor culture pond of a certain farmer in Shandong arbor village, the area of the outdoor culture pond is about 6 mu, the pond is used for culturing prawns, and the initial value of the vibrio cholerae in the culture pond is 106About cfu/ml, adding the mutant strain YJY20-01 in one water pool, adding the centrifugal thallus after strain fermentation into a culture pool, and adjusting the concentration to be 105cfu/ml, another pool plus a cityAnd (3) sprinkling a vibrio sale inhibiting medicament (the main component of which is bacillus subtilis) according to the medicament specification, and continuously tracking and detecting for 7 d.
The experimental result is shown in figure 3, the number of vibrio cholerae in the mutant strain YJY20-01 group is always in a descending trend, the vibrio cholerae in the system can not be basically detected from the 2 nd day after the microbial inoculum is added, the inhibition effect of the mutant strain on the vibrio cholerae is stable, the number of the vibrio cholerae in the water body of the first 3d medicament adding group is lower, and the bacterial quantity is approximately doubled and increased from the 4 th day, so that the continuity and the stability of the antagonistic action of the strain on the vibrio cholerae are better than those of similar vibrio cholerae medicaments in the market.
Sequence listing
<110> Jingbo chemical research institute of yellow river delta Ltd
<120> Vibrio cholerae antagonistic strain and application thereof
<160> 1
<170> SIPOSequenceListing 1.0
<210> 1
<211> 1418
<212> DNA
<213> Bacillus subtilis
<400> 1
acatgcagtc gagcggacag atgggagctt gctccctgat gttagcggcg gacgggtgag 60
taacacgtgg gtaacctgcc tgtaagactg ggataactcc gggaaaccgg ggctaatacc 120
ggatggttgt ttgaaccgca tggttcaaac ataaaaggtg gcttcggcta ccacttacag 180
atggacccgc ggcgcattag ctagttggtg aggtaacggc tcaccaaggc aacgatgcgt 240
agccgacctg agagggtgat cggccacact gggactgaga cacggcccag actcctacgg 300
gaggcagcag tagggaatct tccgcaatgg acgaaagtct gacggagcaa cgccgcgtga 360
gtgatgaagg ttttcggatc gtaaagctct gttgttaggg aagaacaagt accgttcgaa 420
tagggcggta ccttgacggt acctaaccag aaagccacgg ctaactacgt gccagcagcc 480
gcggtaatac gtaggtggca agcgttgtcc ggaattattg ggcgtaaagg gctcgcaggc 540
ggtttcttaa gtctgatgtg aaagcccccg gctcaaccgg ggagggtcat tggaaactgg 600
ggaacttgag tgcagaagag gagagtggaa ttccacgtgt agcggtgaaa tgcgtagaga 660
tgtggaggaa caccagtggc gaaggcgact ctctggtctg taactgacgc tgaggagcga 720
aagcgtgggg agcgaacagg attagatacc ctggtagtcc acgccgtaaa cgatgagtgc 780
taagtgttag ggggtttccg ccccttagtg ctgcagctaa cgcattaagc actccgcctg 840
gggagtacgg tcgcaagact gaaactcaaa ggaattgacg ggggcccgca caagcggtgg 900
agcatgtggt ttaattcgaa gcaacgcgaa gaaccttacc aggtcttgac atcctctgac 960
aatcctagag ataggacgtc cccttcgggg gcagagtgac aggtggtgca tggttgtcgt 1020
cagctcgtgt cgtgagatgt tgggttaagt cccgcaacga gcgcaaccct tgatcttagt 1080
tgccagcatt cagttgggca ctctaaggtg actgccggtg acaaaccgga ggaaggtggg 1140
gatgacgtca aatcatcatg ccccttatga cctgggctac acacgtgcta caatggacag 1200
aacaaagggc agcgaaaccg cgaggttaag ccaatcccac aaatctgttc tcagttcgga 1260
tcgcagtctg caactcgact gcgtgaagct ggaatcgcta gtaatcgcgg atcagcatgc 1320
cgcggtgaat acgttcccgg gccttgtaca caccgcccgt cacaccacga gagtttgtaa 1380
cacccgaagt cggtgaggta acctttagga gccagccg 1418
Claims (5)
1. A cholera vibrio antagonistic strain is characterized by being bacillus subtilis (Bacillus subtilis)Bacillus subtills) YJY20-01, the strain is preserved in China general microbiological culture Collection center in 6 months in 2020, and the preservation number is CGMCC No. 20013.
2. Use of the Vibrio cholerae antagonist strain of claim 1 for antagonizing Vibrio cholerae in aquaculture.
3. The method for applying the vibrio cholerae antagonistic strain in aquaculture as claimed in claim 1, wherein the strain is directly thrown into aquaculture water, or the strain is subjected to expanded culture to prepare a microbial inoculum product and then thrown into aquaculture water.
4. The method of using the antagonistic strain of Vibrio cholerae in aquaculture of claim 3, wherein the initial density of the antagonistic strain of Vibrio cholerae in the aquaculture water is 0.01-1 times the density of Vibrio cholerae.
5. The method of using the antagonistic strain of Vibrio cholerae in aquaculture of claim 4, wherein the initial density of the antagonistic strain of Vibrio cholerae in the aquaculture water is 0.01-0.1 times the density of Vibrio cholerae.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010747624.5A CN111849825B (en) | 2020-07-29 | 2020-07-29 | Vibrio cholerae antagonistic strain and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010747624.5A CN111849825B (en) | 2020-07-29 | 2020-07-29 | Vibrio cholerae antagonistic strain and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN111849825A CN111849825A (en) | 2020-10-30 |
| CN111849825B true CN111849825B (en) | 2022-03-04 |
Family
ID=72946056
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010747624.5A Active CN111849825B (en) | 2020-07-29 | 2020-07-29 | Vibrio cholerae antagonistic strain and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN111849825B (en) |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0928831B1 (en) * | 1997-12-10 | 2006-05-03 | Council of Scientific and Industrial Research | Cholera organisms, vaccines and preparation thereof |
| EP2147091A4 (en) * | 2007-04-24 | 2010-12-08 | Kemin Ind Inc | Broad-spectrum antibacterial and antifungal activity of lactobacillus johnsonii d115 |
| CN102994417B (en) * | 2012-10-17 | 2014-06-11 | 苏州市水生动物疫病预防控制中心 | Bacillus pumilus and application thereof in prevention and control of comma bacillus in aquatic product |
| WO2014169046A1 (en) * | 2013-04-09 | 2014-10-16 | Novozymes A/S | Compositions and methods for improving the health of aquatic animals |
| CN106318880B (en) * | 2015-06-15 | 2019-09-20 | 中国科学院微生物研究所 | One bacillus amyloliquefaciens, bacteriostatic agent prepared therefrom and purposes |
| CN108865953B (en) * | 2018-07-25 | 2021-07-13 | 中国海洋大学 | Bacillus for broad-spectrum inhibition of aquatic pathogenic vibrios and composite bacterium preparation thereof |
| CN110029074B (en) * | 2019-03-15 | 2020-12-29 | 河南科技大学 | Bacillus subtilis and application thereof in prevention and treatment of fish and shrimp culture diseases |
-
2020
- 2020-07-29 CN CN202010747624.5A patent/CN111849825B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN111849825A (en) | 2020-10-30 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Hjelm et al. | Seasonal incidence of autochthonous antagonistic Roseobacter spp. and Vibrionaceae strains in a turbot larva (Scophthalmus maximus) rearing system | |
| CN108251335B (en) | Enterococcus faecalis HKF7 with lactic acid activity and screening culture method and application thereof | |
| CN110029074B (en) | Bacillus subtilis and application thereof in prevention and treatment of fish and shrimp culture diseases | |
| CN109055268B (en) | Composite microecological preparation and application thereof in bee breeding process | |
| CN104164392B (en) | One bacillus licheniformis and the application in aquaculture thereof | |
| CN113040390B (en) | Probiotic salt-tolerant lactobacillus johnsonii and application thereof in preventing and treating pathogenic bacteria in livestock and poultry aquiculture | |
| CN111394272A (en) | Brevibacillus laterosporus and application thereof | |
| CN104164388B (en) | One strain bacillus megaterium and the application in aquaculture thereof | |
| CN115505546B (en) | Salt-tolerant bacillus for inhibiting aeromonas hydrophila and application thereof | |
| CN113652373A (en) | Bacillus and application thereof | |
| CN114437964B (en) | Bacillus belicus strain and application thereof | |
| CN113151035B (en) | Bacillus amyloliquefaciens, screening method, identification method and application | |
| Prihanto et al. | Autochthonous acid-producing bacteria from catfish (Clarias sp.) with antibacterial activity against selected fish pathogens: a preliminary study | |
| CN111849825B (en) | Vibrio cholerae antagonistic strain and application thereof | |
| CN114774310B (en) | Pseudomonas strain and application thereof | |
| Ebnetorab et al. | Isolation, biochemical and molecular detection of Bacillus subtilis and Bacillus licheniformis from the digestive system of rainbow trout (Oncorhynchus mykiss) and its inhibitory effect on Aeromonas hydrophila | |
| CN115820482A (en) | Bacillus amyloliquefaciens YN-BA2 and microbial inoculum and application thereof | |
| CN109897800A (en) | The strong enterococcus A8-1 of one plant of selenium-rich and its application | |
| CN114657082B (en) | Lactococcus lactis and application thereof | |
| CN113604387B (en) | Salt-tolerant and high-temperature-resistant lactobacillus reuteri and application thereof in prevention and treatment of pathogenic bacteria in livestock and poultry aquaculture | |
| CN113528375B (en) | Min swine bacillus subtilis capable of inhibiting growth of various pathogenic bacteria microorganisms | |
| CN115216434A (en) | Lactobacillus salivarius strain and application thereof | |
| CN113308397A (en) | Probiotics for inhibiting clostridium perfringens and screening method and application thereof | |
| CN114621884A (en) | Bacillus subtilis and application thereof in water purification | |
| CN111647523B (en) | Probiotic composition for stichopus japonicus culture and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |