CN111837809A - Black termitomyces albuminosus culture medium and preparation method and application thereof - Google Patents
Black termitomyces albuminosus culture medium and preparation method and application thereof Download PDFInfo
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- CN111837809A CN111837809A CN202010732378.6A CN202010732378A CN111837809A CN 111837809 A CN111837809 A CN 111837809A CN 202010732378 A CN202010732378 A CN 202010732378A CN 111837809 A CN111837809 A CN 111837809A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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Abstract
The invention is suitable for the technical field of black termitomyces albuminosus culture, and provides a black termitomyces albuminosus culture medium, a preparation method and application thereof.
Description
Technical Field
The invention belongs to the technical field of black termitomyces albuminosus culture, and particularly relates to a black termitomyces albuminosus culture medium and a preparation method and application thereof.
Background
The black termitomyces albuminosus is a very special large fungus parasitic in soil around the termite nest, is white in color, tender in meat quality, fragrant and all around, crisp in taste and is famous wild edible fungus. The black termitomyces albuminosus contains essential amino acids, proteins, fats, vitamins, calcium, phosphorus, and retinoic acid.
The black collybia albuminosa artificial culture fruiting difficulty is very large, the fruiting is difficult when the nutritional characteristics in the culture material do not meet the characteristic requirements, the culture medium is prepared by taking sawdust and cottonseed hulls as main materials in the prior art for cultivating the black collybia albuminosa, the forest fungi contradiction can occur in large-scale production, the cost is high, and the sustainable development environment protection is not facilitated
Disclosure of Invention
The invention provides a black termitomyces albuminosus culture medium, a preparation method and application thereof, and aims to solve the problems in the prior art.
The invention is realized in such a way that the black termitomyces albuminosus culture medium is prepared from the following substances in parts by mass: 10 to 15 parts of wood chips, 5 to 9 parts of corncobs, 5 to 7 parts of yeast powder, 12 to 18 parts of sugar, 15 to 20 parts of wheat bran, 2 to 3 parts of monopotassium phosphate and 928 to 951 parts of water.
Preferably, the feed additive is prepared from the following materials in parts by mass: 10 parts of wood chips, 5 parts of corncobs, 5 parts of yeast powder, 12 parts of sugar, 15 parts of wheat bran, 2 parts of monopotassium phosphate and 951 parts of water.
Preferably, the feed additive is prepared from the following materials in parts by mass: 15 parts of wood chips, 9 parts of corncobs, 7 parts of yeast powder, 18 parts of sugar, 20 parts of wheat bran, 3 parts of monopotassium phosphate and 928 parts of water.
Preferably, the sugar is sucrose or glucose.
The invention also provides a preparation method of any one of the black termitomyces albuminosus culture media, which comprises the following steps:
s1, weighing sawdust, corncobs, yeast powder, sugar, wheat bran, monopotassium phosphate and water according to weight, heating the water to the water temperature of 50-60 ℃, adding the corncobs and the sawdust to dilute the water to obtain a diluent, sequentially adding the sugar, cottonseed hulls, the yeast powder, the wheat bran, the monopotassium phosphate, magnesium sulfate and the red date powder, stirring the mixture to be completely uniform to obtain a mixed solution, continuously heating the mixed solution to boiling, and fully stirring the mixed solution to be uniform;
s2, filling the uniformly stirred mixed solution into a container, and sealing;
s3, hermetically fermenting the well-sealed mixed solution at normal temperature for 3-4 months to obtain liquid fermentation nutrient solution;
s4, filtering the liquid fermentation nutrient solution to obtain filtrate, namely the black termitomyces albuminosus culture medium.
The invention also provides a cultivation method of black termitomyces albuminosus, which comprises the following steps:
a. subpackaging the culture medium for Collybia albuminosa according to any one of claims 1 to 4 into culture bottles;
b. sterilizing the black termitomyces albuminosus culture medium filled into the culture bottle for 1 to 2 hours at the high temperature of 110 to 120 ℃ to obtain the sterilized black termitomyces albuminosus culture medium;
c. b, inoculating a strain cultured by the black termitomyces albuminosus mother strain to the sterilized black termitomyces albuminosus culture medium obtained in the step b in an aseptic environment;
d. placing the inoculated culture bottle in the dark, culturing for 6-7 days at the temperature of 10-30 ℃ and the humidity of 50-60% until the culture medium is full of hyphae;
e. culturing for 40-50 days under the dark light condition that the temperature is 20-25 ℃ and the air humidity is 50-80% until the hyphae grow to be mature.
Preferably, the diameter of the cultivation bottle is 20 cm-25 cm, and the height of the cultivation bottle is 10 cm-15 cm.
Preferably, the height of the black termitomyces albuminosus culture medium filled into the culture bottle is 8-12 cm.
Compared with the prior art, the invention has the beneficial effects that: the black termitomyces albuminosus culture medium is prepared by arranging the culture medium prepared from wood chips, corncobs, yeast powder, sugar, wheat bran, monopotassium phosphate and water, heating, mixing, stirring, fermenting and filtering the culture medium to obtain filtrate as the culture medium, and sterilizing, inoculating and culturing the culture medium to obtain mature hyphae.
Drawings
FIG. 1 is a schematic flow chart of the preparation method of the black termitomyces albuminosus culture medium of the present invention.
FIG. 2 is a schematic flow chart of the culture method of Collybia albuminosa according to the present invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is described in further detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
Example one
The embodiment provides a technical scheme: the black termitomyces albuminosus culture medium is characterized in that: the medicine is prepared from the following substances in parts by mass: 10 parts of wood chips, 5 parts of corncobs, 5 parts of yeast powder, 12 parts of sugar, 15 parts of wheat bran, 2 parts of monopotassium phosphate and 951 parts of water.
Wherein the sugar is sucrose or glucose.
Referring to FIG. 1, a Black Collybia albuminosa culture medium was then prepared according to the following steps:
s1, weighing the wood chips, the corncobs, the yeast powder, the sugar, the wheat bran, the monopotassium phosphate and the water according to the weight, heating the water to the water temperature of 50-60 ℃, adding the corncobs and the wood chips for dilution to obtain a diluent, sequentially adding the sugar, the cottonseed hulls, the yeast powder, the wheat bran, the monopotassium phosphate, the magnesium sulfate and the red date powder, stirring to be completely uniform to obtain a mixed solution, and continuously heating the mixed solution to boiling and then fully stirring to be uniform.
And S2, filling the uniformly stirred mixed liquid into a container, and sealing.
And S3, hermetically fermenting the well-sealed mixed solution at normal temperature for 3-4 months to obtain the liquid fermentation nutrient solution.
S4, filtering the liquid fermentation nutrient solution to obtain filtrate, namely the black termitomyces albuminosus culture medium.
Referring to FIG. 2, the above-mentioned medium was then used to culture Collybia albuminosa according to the following procedure:
a. and subpackaging the black termitomyces albuminosus culture medium into culture bottles. The diameter of the cultivation bottle is 20cm, and the height is 10 cm. The height of the black termitomyces albuminosus culture medium filled into the culture bottle is 8 cm.
b. And maintaining the black termitomyces albuminosus culture medium filled into the culture bottle for sterilization for 1 to 2 hours under the high temperature condition of 110 to 120 ℃ to obtain the sterilized black termitomyces albuminosus culture medium.
c. And (c) inoculating a strain cultured by the black termitomyces albuminosus mother strain to the sterilized black termitomyces albuminosus culture medium obtained in the step (b) in an aseptic environment.
d. Placing the inoculated culture bottle in the dark, at the temperature of 10-30 ℃ and the humidity of 50-60%, and culturing for 6-7 days until the culture medium is full of hyphae.
e. Culturing for 40-50 days under the dark light condition that the temperature is 20-25 ℃ and the air humidity is 50-80% until the hyphae grow to be mature.
The black termitomyces albuminosus planted by the culture medium has uniform shape and size of hyphae, high water content and good taste. The dried black termitomyces albuminosus has crude protein content of 30.8%, total sugar content of 25.6%, crude fat content of 4.3% and crude fiber content of 6.3%.
The black termitomyces albuminosus culture medium is prepared by arranging the culture medium prepared from wood chips, corncobs, yeast powder, sugar, wheat bran, monopotassium phosphate and water, heating, mixing, stirring, fermenting and filtering the culture medium to obtain filtrate as the culture medium, and sterilizing, inoculating and culturing the culture medium to obtain mature hyphae.
Example two
The embodiment provides a technical scheme: the black termitomyces albuminosus culture medium is characterized in that: the medicine is prepared from the following substances in parts by mass: 12 parts of wood chips, 7 parts of corncobs, 6 parts of yeast powder, 15 parts of sugar, 17 parts of wheat bran, 2 parts of monopotassium phosphate and 941 parts of water.
Wherein the sugar is sucrose or glucose.
Then preparing the black termitomyces albuminosus culture medium according to the following steps:
s1, weighing the wood chips, the corncobs, the yeast powder, the sugar, the wheat bran, the monopotassium phosphate and the water according to the weight, heating the water to the water temperature of 50-60 ℃, adding the corncobs and the wood chips for dilution to obtain a diluent, sequentially adding the sugar, the cottonseed hulls, the yeast powder, the wheat bran, the monopotassium phosphate, the magnesium sulfate and the red date powder, stirring to be completely uniform to obtain a mixed solution, and continuously heating the mixed solution to boiling and then fully stirring to be uniform.
And S2, filling the uniformly stirred mixed liquid into a container, and sealing.
And S3, hermetically fermenting the well-sealed mixed solution at normal temperature for 3-4 months to obtain the liquid fermentation nutrient solution.
S4, filtering the liquid fermentation nutrient solution to obtain filtrate, namely the black termitomyces albuminosus culture medium.
Then culturing the black termitomyces albuminosus by using the culture medium according to the following steps:
a. and subpackaging the black termitomyces albuminosus culture medium into culture bottles. The diameter of the cultivation bottle is 22cm, and the height is 12 cm. The height of the black termitomyces albuminosus culture medium filled into the culture bottle is 10 cm.
b. And maintaining the black termitomyces albuminosus culture medium filled into the culture bottle for sterilization for 1 to 2 hours under the high temperature condition of 110 to 120 ℃ to obtain the sterilized black termitomyces albuminosus culture medium.
c. And (c) inoculating a strain cultured by the black termitomyces albuminosus mother strain to the sterilized black termitomyces albuminosus culture medium obtained in the step (b) in an aseptic environment.
d. Placing the inoculated culture bottle in the dark, at the temperature of 10-30 ℃ and the humidity of 50-60%, and culturing for 6-7 days until the culture medium is full of hyphae.
e. Culturing for 40-50 days under the dark light condition that the temperature is 20-25 ℃ and the air humidity is 50-80% until the hyphae grow to be mature.
The black termitomyces albuminosus planted by the culture medium has uniform shape and size of hyphae, high water content and good taste. The dried black termitomyces albuminosus has the crude protein content of 33.8 percent, the total sugar content of 26.3 percent, the crude fat content of 4.5 percent and the crude fiber content of 6.1 percent.
EXAMPLE III
The embodiment provides a technical scheme: the black termitomyces albuminosus culture medium is characterized in that: the medicine is prepared from the following substances in parts by mass: 15 parts of wood chips, 9 parts of corncobs, 7 parts of yeast powder, 18 parts of sugar, 20 parts of wheat bran, 3 parts of monopotassium phosphate and 928 parts of water.
Wherein the sugar is sucrose or glucose.
Then preparing the black termitomyces albuminosus culture medium according to the following steps:
s1, weighing the wood chips, the corncobs, the yeast powder, the sugar, the wheat bran, the monopotassium phosphate and the water according to the weight, heating the water to the water temperature of 50-60 ℃, adding the corncobs and the wood chips for dilution to obtain a diluent, sequentially adding the sugar, the cottonseed hulls, the yeast powder, the wheat bran, the monopotassium phosphate, the magnesium sulfate and the red date powder, stirring to be completely uniform to obtain a mixed solution, and continuously heating the mixed solution to boiling and then fully stirring to be uniform.
And S2, filling the uniformly stirred mixed liquid into a container, and sealing.
And S3, hermetically fermenting the well-sealed mixed solution at normal temperature for 3-4 months to obtain the liquid fermentation nutrient solution.
S4, filtering the liquid fermentation nutrient solution to obtain filtrate, namely the black termitomyces albuminosus culture medium.
Then culturing the black termitomyces albuminosus by using the culture medium according to the following steps:
a. and subpackaging the black termitomyces albuminosus culture medium into culture bottles. The diameter of the cultivation bottle is 25cm, and the height is 15 cm. The height of the black termitomyces albuminosus culture medium filled into the culture bottle is 13 cm.
b. And maintaining the black termitomyces albuminosus culture medium filled into the culture bottle for sterilization for 1 to 2 hours under the high temperature condition of 110 to 120 ℃ to obtain the sterilized black termitomyces albuminosus culture medium.
c. And (c) inoculating a strain cultured by the black termitomyces albuminosus mother strain to the sterilized black termitomyces albuminosus culture medium obtained in the step (b) in an aseptic environment.
d. Placing the inoculated culture bottle in the dark, at the temperature of 10-30 ℃ and the humidity of 50-60%, and culturing for 6-7 days until the culture medium is full of hyphae.
e. Culturing for 40-50 days under the dark light condition that the temperature is 20-25 ℃ and the air humidity is 50-80% until the hyphae grow to be mature.
The black termitomyces albuminosus planted by the culture medium has uniform shape and size of hyphae, high water content and good taste. The dried black termitomyces albuminosus has a crude protein content of 32.3%, total sugar content of 24.5%, crude fat content of 4.2% and crude fiber content of 5.9%.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents and improvements made within the spirit and principle of the present invention are intended to be included within the scope of the present invention.
Claims (8)
1. The black termitomyces albuminosus culture medium is characterized in that: the medicine is prepared from the following substances in parts by mass: 10 to 15 parts of wood chips, 5 to 9 parts of corncobs, 5 to 7 parts of yeast powder, 12 to 18 parts of sugar, 15 to 20 parts of wheat bran, 2 to 3 parts of monopotassium phosphate and 928 to 951 parts of water.
2. The culture medium for Collybia albuminosa according to claim 1, wherein: the medicine is prepared from the following substances in parts by mass: 10 parts of wood chips, 5 parts of corncobs, 5 parts of yeast powder, 12 parts of sugar, 15 parts of wheat bran, 2 parts of monopotassium phosphate and 951 parts of water.
3. The culture medium for Collybia albuminosa according to claim 1, wherein: the medicine is prepared from the following substances in parts by mass: 15 parts of wood chips, 9 parts of corncobs, 7 parts of yeast powder, 18 parts of sugar, 20 parts of wheat bran, 3 parts of monopotassium phosphate and 928 parts of water.
4. The culture medium for Collybia albuminosa according to claim 1, wherein: the sugar is sucrose or glucose.
5. The method for producing the culture medium for Collybia albuminosa according to any one of claims 1 to 4, wherein: the method comprises the following steps:
s1, weighing sawdust, corncobs, yeast powder, sugar, wheat bran, monopotassium phosphate and water according to weight, heating the water to the water temperature of 50-60 ℃, adding the corncobs and the sawdust to dilute the water to obtain a diluent, sequentially adding the sugar, cottonseed hulls, the yeast powder, the wheat bran, the monopotassium phosphate, magnesium sulfate and the red date powder, stirring the mixture to be completely uniform to obtain a mixed solution, continuously heating the mixed solution to boiling, and fully stirring the mixed solution to be uniform;
s2, filling the uniformly stirred mixed solution into a container, and sealing;
s3, hermetically fermenting the well-sealed mixed solution at normal temperature for 3-4 months to obtain liquid fermentation nutrient solution;
s4, filtering the liquid fermentation nutrient solution to obtain filtrate, namely the black termitomyces albuminosus culture medium.
6. A cultivation method of black termitomyces albuminosus is characterized by comprising the following steps: comprises the following steps:
a. subpackaging the culture medium for Collybia albuminosa according to any one of claims 1 to 4 into culture bottles;
b. sterilizing the black termitomyces albuminosus culture medium filled into the culture bottle for 1 to 2 hours at the high temperature of 110 to 120 ℃ to obtain the sterilized black termitomyces albuminosus culture medium;
c. b, inoculating a strain cultured by the black termitomyces albuminosus mother strain to the sterilized black termitomyces albuminosus culture medium obtained in the step b in an aseptic environment;
d. placing the inoculated culture bottle in the dark, culturing for 6-7 days at the temperature of 10-30 ℃ and the humidity of 50-60% until the culture medium is full of hyphae;
e. culturing for 40-50 days under the dark light condition that the temperature is 20-25 ℃ and the air humidity is 50-80% until the hyphae grow to be mature.
7. The method for cultivating Collybia albuminosa according to claim 6, wherein: the diameter of the cultivation bottle is 20 cm-25 cm, and the height of the cultivation bottle is 10 cm-15 cm.
8. The method for cultivating Collybia albuminosa according to claim 7, wherein: the height of the black termitomyces albuminosus culture medium filled into the cultivation bottle is 8-12 cm.
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Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105684727A (en) * | 2016-01-25 | 2016-06-22 | 赵建荣 | Culture medium and cultivation method for cultivating black termitomyces albuminosus with enzyme bacterium residues as main raw material |
| CN105695338A (en) * | 2016-01-25 | 2016-06-22 | 四川保兴现代农业科技股份有限公司 | Liquid spawn propagation method and field bionic cultivation method of termitomyces albuminosus |
| CN106852252A (en) * | 2016-08-12 | 2017-06-16 | 金珠满江农业有限公司 | A kind of black collybia albuminosa artificial bionic domestication and culture method |
| CN107896824A (en) * | 2017-11-29 | 2018-04-13 | 四川山水美地农业投资有限公司 | A kind of cultural method of Termitomyces albuminosus with black skin |
| CN108293589A (en) * | 2017-08-29 | 2018-07-20 | 田林县群英农业有限公司 | A kind of cultural method of collybia albuminosa |
| CN108522136A (en) * | 2018-02-02 | 2018-09-14 | 金珠满江农业有限公司 | Black collybia albuminosa culture medium and the preparation method and application thereof |
| CN109076882A (en) * | 2018-06-29 | 2018-12-25 | 江西天和食用菌开发有限公司 | A kind of mycelial cultural method of selenium-enriched edible mushroom and its application |
-
2020
- 2020-07-27 CN CN202010732378.6A patent/CN111837809A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105684727A (en) * | 2016-01-25 | 2016-06-22 | 赵建荣 | Culture medium and cultivation method for cultivating black termitomyces albuminosus with enzyme bacterium residues as main raw material |
| CN105695338A (en) * | 2016-01-25 | 2016-06-22 | 四川保兴现代农业科技股份有限公司 | Liquid spawn propagation method and field bionic cultivation method of termitomyces albuminosus |
| CN106852252A (en) * | 2016-08-12 | 2017-06-16 | 金珠满江农业有限公司 | A kind of black collybia albuminosa artificial bionic domestication and culture method |
| CN108293589A (en) * | 2017-08-29 | 2018-07-20 | 田林县群英农业有限公司 | A kind of cultural method of collybia albuminosa |
| CN107896824A (en) * | 2017-11-29 | 2018-04-13 | 四川山水美地农业投资有限公司 | A kind of cultural method of Termitomyces albuminosus with black skin |
| CN108522136A (en) * | 2018-02-02 | 2018-09-14 | 金珠满江农业有限公司 | Black collybia albuminosa culture medium and the preparation method and application thereof |
| CN109076882A (en) * | 2018-06-29 | 2018-12-25 | 江西天和食用菌开发有限公司 | A kind of mycelial cultural method of selenium-enriched edible mushroom and its application |
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