CN111837696B

CN111837696B - Method for producing agilawood powder and essential oil by using aquilaria sinensis aerial aeroponic bacon

Info

Publication number: CN111837696B
Application number: CN202010559096.0A
Authority: CN
Inventors: 马新业; 肖乃玉; 蓝碧锋; 钟乐; 张雪琴
Original assignee: Guangzhou University of Traditional Chinese Medicine
Current assignee: Guangzhou University of Traditional Chinese Medicine
Priority date: 2020-06-18
Filing date: 2020-06-18
Publication date: 2021-11-19
Anticipated expiration: 2040-06-18
Also published as: CN111837696A

Abstract

The invention discloses a method for producing agilawood powder and essential oil by utilizing aquilaria sinensis aerial fog bacon, which comprises the following steps: (1) cultivating the aquilaria sinensis seedling type suitable for the aerial fog cultivation environment; (2) selecting qualified aquilaria sinensis seedlings, transferring the aquilaria sinensis seedlings to an aerial fog environment for long-term culture, and continuously harvesting regenerated aerial fog roots in a multi-time stubble cutting mode; (3) soaking or spraying in vitro aerial fog root with lignum Aquilariae Resinatum inducer to generate sesquiterpene and chromone compounds in radix Aristolochice, and making into lignum Aquilariae Resinatum powder and essential oil. The method continuously harvests the regenerated aerial fog root material in a multi-time stubble cutting mode, combines the soaking or spraying of the agilawood inducer to induce the root to generate the characteristic component of the agilawood formation marker with a specific variety, can obviously shorten the agilawood production time, obviously improves the utilization rate of raw materials, and improves the agilawood yield and quality stability.

Description

Method for producing agilawood powder and essential oil by using aquilaria sinensis aerial aeroponic bacon

Technical Field

The invention belongs to the technical field of agilawood, and particularly relates to a method for producing agilawood powder and essential oil by utilizing aquilaria sinensis aerial aeroponic radices.

Background

At present, wild traditional Chinese medicine resources are exhausted due to excessive harvesting and ecological environment deterioration, environmental pollution, production order failure and the like also cause the quality of traditional planted traditional Chinese medicine to be reduced and potential safety hazards to be increased, so that the modern agricultural technology is combined with the multidisciplinary frontier technology, the first-level development of new resources is carried out on the basis of fully considering the specificity of the traditional Chinese medicine and the actual productivity level of traditional Chinese medicine agriculture at the present stage, and the necessity and the urgency are more highlighted at present.

Aquilaria sinensis, a resin-containing wood of various species of Aquilaria and Aquilaria of Thymelaeaceae (the only genuine basic plant of Aquilaria sinensis produced in China is Aquilaria sinensis, also known as native Aquilaria sinensis), is a traditional Chinese medicinal material and a world-famous perfume in China, Japan, India and southeast Asia. At present, wild agilawood resources are gradually exhausted worldwide, and the international trade of the wild agilawood resources is controlled by 'International trade convention of endangered wild animal and plant species CITES'. In order to realize the sustainable utilization of resources, the artificial planting area of aquilaria sinensis is continuously enlarged as a medicinal material in the south-south China where wild resources are endangered, and the artificial incense producing technology is continuously innovated and optimized. However, various existing technologies such as 'bottle making' and 'fire hole drilling' have the problems of thin-layer incense formation, wood decay and the like, the supply cycle of the incense formation resources of the big trees needs at least nearly ten years on the whole, and the production enthusiasm of practitioners is also seriously influenced by the reasons of low resource utilization rate, low investment return and the like. The artificial agilawood producing technology is still a bottleneck restricting the development of the agilawood industry, and the alleviation of the contradiction between supply and demand is still an inherent strong power for developing new agilawood resources.

In view of the biological characteristics of more aquilaria sinensis seeds, simple seedling raising technology, rapid growth and the like, the method can be developed for harvesting root materials in a short period of aquilaria sinensis seedling aerosol cultivation in an artificial environment, and then carrying out stress and incense formation by taking the roots as objects, and is a potential method for producing agilawood and additional products (such as incense powder and essential oil) thereof.

Disclosure of Invention

The invention aims to provide a method for producing agilawood powder and essential oil by utilizing aquilaria sinensis aerial fog bacon.

The first object of the present invention can be achieved by the following technical solutions: a method for producing agilawood powder and essential oil by using aquilaria sinensis aerial aeroponic bacon comprises the following steps:

(1) cultivating the aquilaria sinensis seedling type suitable for the aerial fog cultivation environment;

(2) selecting qualified aquilaria sinensis seedlings, transferring the aquilaria sinensis seedlings to an aerial fog environment for long-term culture, and continuously harvesting regenerated aerial fog roots in a multi-time stubble cutting mode;

(3) soaking or spraying in vitro aerial fog root with lignum Aquilariae Resinatum inducer to generate sesquiterpene and chromone compounds in radix Aristolochice, and making into lignum Aquilariae Resinatum powder and essential oil.

In view of the biological characteristics of more aquilaria sinensis seeds, simple seedling raising technology, rapid growth and the like, the method develops the modern agricultural technical research of aquilaria sinensis seedling aerosol cultivation under the condition of artificial environment, can harvest regenerated aerosol root materials in a short period and continuously, excavates and applies the biological principle, and combines the technologies of high molecular functional materials, sustained and controlled release and the like to develop a novel agilawood inducer to induce the aerosol root to generate species-specific incense forming marker components (such as sesquiterpenes and chromone substances). The method for developing new agilawood resources has the advantages of obviously reducing the time and space cost required by the production of traditional resources, greatly improving the utilization rate of raw materials, obviously improving the safety and quality stability of products, being environment-friendly and the like, and has a good prospect of improving the market supply condition of medicinal materials/spices of the product.

Compared with the prior agilawood producing technology, the method can obviously excavate the biological potential of the aquilaria sinensis with smaller space-time cost, realizes stable yield of mass production, and is a new idea for developing agilawood resources with high added value by jointly applying modern agricultural soilless culture technology and multi-disciplinary frontier technologies such as functional materials, sustained and controlled release and the like. The fundamental biological principle of the invention is that the novel agilawood inducer can generate a certain intensity of adversity stimulation conditions, induce aquilaria sinensis in a proper physiological state in vivo or in vitro living cells to generate defense reaction, activate a specific secondary metabolic pathway of the species, finally generate agilawood formation marking components such as sesquiterpenes and chromones, and then prepare agilawood powder and essential oil by adopting the conventional technology in the field, so that the effective supply of agilawood medicinal material/spice resources can be improved.

In the method for producing agilawood powder and essential oil by using aquilaria sinensis aerial fog bacon:

in the step (1) of the present application, a conventional technology which can be understood by those skilled in the art can be utilized to carry out aquilaria sinensis seed seedling cultivation, seedling cultivation can adopt a seedling bag for seedling cultivation, but specific measures (such as seed germination in a straight round paper cylinder to ensure that a main root is upright, such as chlormequat chloride and/or topping pruning to limit the height of the main stem to grow and increase the crown width and the like) are needed to be added to obtain a specific plant type suitable for transplanting an aerial fog culture environment, namely, characteristics such as root system, plant height, branches, crown width, leaves and the like are required, the principle aims are that the main root is upright, the plant planting is easy, the plant is uniformly distributed in the aerial fog environment, the plant height is suitable for plant planting, operations such as bearing of cultivation equipment, covering and moisture preservation, and the like, and the branch, the number of leaves are multiple, and the crown width can ensure the photosynthetic metabolism center strength of overground part.

In particular, these specific measures include: the main measures different from the conventional aquilaria sinensis seed seedling cultivation program are as follows: (A) the seeds are sleeved in the middle of the seedling bag by a breathable, permeable and cylindrical hollow paper tube so as to ensure that the main roots for germination are upright; (B) measures such as chlormequat chloride and/or topping and pruning are utilized to limit the height of the seedling trunk, increase branches and increase the crown width.

Preferably, the aquilaria sinensis seedlings in the step (1) have developed plant type integral roots and upright main roots, branches and large crown width, deep green leaves and a large number of leaves, and have higher plant height than that of the conventional earth culture seedlings which are not specially treated.

Preferably, the desirable characteristics of aquilaria sinensis seedlings in step (2) include: the number of thin roots is large, the main root is upright and has a length of more than 15cm, the ground diameter is 0.5 +/-0.1 cm, the plant height is 50 +/-10 cm, more than 2 branches are provided, the number of leaves is large, the leaves are active (dark green), and the seedling age is 1.5-2.5 years.

Preferably, the step (2) of continuously harvesting the regenerated aerial fog roots in a multi-stubble cutting mode comprises the following steps:

(2.1) cutting all or part of aerial fog roots regenerated on the main roots of the aquilaria sinensis seedlings from the root base parts, and using the obtained in-vitro aerial fog roots for subsequent fragrance formation induction;

(2.2) spraying a rooting inducer on the main roots of the aquilaria sinensis seedlings, then carrying out aerial fog culture by using pure water until adventitious roots can be seen by naked eyes, and then carrying out aerial fog root growth culture by using nutrient solution;

and (2.3) repeating the steps (2.1) to (2.2) to obtain the regenerated aerosol roots for multiple times.

Preferably, the length of the aerosol root regenerated in the steps (2.1) to (2.2) is 15-25 cm.

Preferably, the rooting inducer in the step (2.2) is one or more of 6-benzylamino adenine, indolebutyric acid, naphthylacetic acid and commercial ABT rooting powder; wherein the commercial ABT rooting powder can be selected into specific types according to requirements.

Preferably, in the step (2.2), the transpiration of the overground part needs to be reduced before the aerial fog roots are regenerated by naked eyes and before the aerial fog roots are transferred to a nutrient solution for growth and culture, and measures comprise spraying a transpiration inhibitor on the leaves, spraying water on the leaves regularly or closing the small plant growth environment relatively and the like.

Preferably, the agilawood inducer in the step (3) is attached to the surface of the aerosol root in a soaking or spraying manner, and the agilawood inducer comprises a basement membrane, agilawood induction factors, a carrier, inorganic salts, saccharides and organic acids.

Preferably, the mass parts of the basement membrane, the agilawood induction factor, the carrier, the inorganic salt, the saccharide and the organic acid are respectively 5-50: 0.1-1.5: 0.5-4.2: 0.12-0.4: 0.1-0.3: 0.08 to 0.2.

Preferably, in the step (3), the basement membrane comprises one or more of ethylene-vinyl acetate copolymer, cellulose derivatives, soluble chitosan and shellac, the agilawood inducing factor comprises one or more of ethephon, 6-benzylaminopurine and methyl jasmonate, the carrier comprises one or more of cyclodextrin, ethyl cellulose and sodium carboxymethyl cellulose, the inorganic salt comprises one or two of sodium chloride and potassium chloride, the saccharide comprises one or two of sucrose or glucose, and the organic acid comprises one or two of malic acid, citric acid and salicylic acid.

The agilawood inducer can be prepared by a conventional method, for example, the agilawood inducer can be directly and uniformly mixed for use, but the preparation method provided in the embodiment of the invention is recommended to be adopted.

The preparation method of the preferred agilawood inducer comprises the following steps: selecting a carrier 1 to dissolve in water, adding an agilawood induction factor, heating in a water bath, dispersing in the carrier 2 again to completely dissolve the carrier, sequentially adding an organic acid, a saccharide and an inorganic salt, and adding a base membrane to obtain the agilawood inducer, wherein the carrier 1 is different from the carrier 2.

Preferably, in the step (3), the isolated roots are soaked or sprayed with the agilawood inducer, and the isolated roots stand in a cool and ventilated place to form a film on the surfaces of the isolated roots.

Preferably, after the surface film is formed, the aerosol roots are placed in a clean, breathable and dark environment (preferably in an incubator), and the humidity of the environment is adjusted on the basis of dehydration of the aerosol roots to constant weight within 1-2 weeks, so that the aerosol roots are dried to constant weight.

Preferably, in order to prevent mildew during the process of generating fragrance by forming a film on the surface of the isolated aerosol root and generating fragrance-forming marker compounds, namely sesquiterpenes and chromones, in the aquilaria sinensis root, ozone, ultraviolet rays, hydrogen peroxide or potassium permanganate solution is adopted for disinfection treatment.

Preferably, in the step (3), the formation of the fragrant marked compounds of sesquiterpenes and chromone substances is generated in the aquilaria sinensis roots, and then the complete aquilaria sinensis aerial fog root material is directly ground into powder to prepare the aquilaria sinensis powder, or the complete aquilaria sinensis aerial fog root material is further utilized to extract essential oil by a conventional method, and the essential oil can be obtained by a conventional method in the field.

Compared with the prior art, the invention has the following advantages:

(1) estimated according to the existing artificial production mode of whole body or whole tree fragrance formation, white wood fragrance trees are planted for at least 5 years, the planting distance is 1 meter and half multiplied by 1 meter and half, and fragrance formation is induced for at least half a year to obtain 1 kilogram of fragrance tablets, while the development mode of the new resources for aerial fog cultivation in the application can accommodate 100 small seedlings in the same area even according to the design of single-layer planar cultivation, 1 year is consumed for at most 10 cultivation cycles (or 1 month is consumed for 1 cultivation cycle in 10 times area), and 1 kilogram of fragrance can be obtained, namely, the productivity of the new mode is at least 5 times improved compared with that of the traditional mode;

(2) the development of new resources in an aerosol mode is not limited by regional environments, pesticide residues, heavy metals/toxic microorganisms can be avoided to the greatest extent, the production mode is ecological and environment-friendly, a large amount of time, space and labor cost can be saved, the technological content of industrial production is improved, the resource supply linkage is improved, the development of traditional Chinese medicine agriculture, traditional Chinese medicine industry and large health industry is promoted, and the economic development of agricultural rural areas is driven;

(3) the method can realize the sustained and controlled release of the agilawood inducer, improve the utilization rate of plant materials, realize one-time coating and realize simple and convenient operation;

(4) the method has good reproducibility, is easy to realize standardization and commercialization of the agilawood product, has stable fragrance, and can widen the application of the agilawood in daily chemicals, cigarettes and other fast-elimination products.

Drawings

FIG. 1 is a fresh aerosol of the coating film treated in examples 1-3;

FIG. 2 shows the finished roots of examples 1-3 after treatment with the novel edgeworthia chrysantha-inducing agent, containing the marker edgeworthia components (finished, i.e., the finished roots with edgeworthia chrysantha, which can be broken into incense powders or extracted to obtain essential oils);

FIG. 3 is a GC total ion flow graph of the isolated root chemistry after induction in examples 1-3;

FIG. 4 is a graph showing the MS identification results of 2- (2-phenylethyl) chromone, a key component in the chemical composition of the excised roots after induction in examples 1-3;

FIG. 5 is a graph of MS identification of sesquiterpene substances, the key component of the chemical composition of the roots ex vivo after induction in examples 1-3.

Detailed Description

The accompanying drawings, which are included to provide a further understanding of the invention and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention and together with the description serve to explain the principles of the invention and not to limit the invention.

The raw materials adopted in the invention are recommended but not limited to food contact materials or food grade raw materials allowed to be used in national food regulations, and meet the requirements of no toxicity, no odor and the like.

Example 1

(1) Seedling raising bag for raising seedlings

The aquilaria sinensis seedling cultivation is carried out by utilizing the conventional technology which can be understood by the technical personnel in the field, but specific measures are required to be added to obtain a specific plant type suitable for transplanting aerial fog cultivation, namely, characteristics such as root systems, plant heights, branches, crown widths, leaves and the like are required, the principle aims are that main roots are upright and are easy to be uniformly distributed in an aerial fog environment, the plant heights are suitable for plant fixing, bearing of cultivation equipment and covering and moisture preserving operation, and the photosynthetic metabolism center strength of overground parts can be ensured by the branches, the number of leaves and the large crown widths.

The special measures different from the conventional aquilaria sinensis seed seedling cultivation program are as follows: (A) the method comprises the following steps of (A) inserting a round paper tube between seedling growing bags to limit the early germination of seeds so as to ensure the uprightness of main roots, (B) utilizing chlormequat chloride (CCC) and a topping pruning measure to ensure the characteristics of plant height, crown width, branches and leaf number.

(2) Multiple root-cutting-rooting cycle in aerial fog cultivation mode

The plant type meeting the requirements is selected to enter an aeroponic culture experiment, and the characteristics are as follows: more fine roots, upright main roots with the length of more than 15cm, ground diameter of 0.5 +/-0.1 cm, plant height of 50 +/-10 cm, more than 2 branches, more leaves, dark green and seedling age of 2 years. It will be appreciated that various agronomic measures may be used to ensure the plant type. In addition, if the plant type is changed, the subsequent aerosol culture facilities and the corresponding adjustment of the aerosol culture operation can be caused, but the aim of aerosol root culture can also be achieved.

Transferring the soil-cultured seedlings to aerial fog culture based on the earlier patent technology (an aerial fog culture method ZL 201510751445.8 of the soil-cultured agilawood), wherein part of operations can be optimized, such as spraying foliar fertilizer (common commercially available foliar fertilizer) and irrigating roots 3 days before the aerial fog culture, so that the seedlings can be fully absorbed to improve the stress resistance, the content of nutrient substances and the rooting potential; the overground part can be covered by a simple transparent plastic film, and a transpiration inhibitor is sprayed to ensure that the transpiration of the stems and leaves is inhibited before rooting. Harvesting when the aerial fog roots of each crop averagely grow to 20cm, cutting off roots from the base during harvesting, then spraying ABT rooting powder No. 3 solution (Beijing Aibidi Biotechnology Co., Ltd.) with recommended use concentration on the surface of the main roots by using a spray can, slightly drying in the air, carrying out aerial fog culture by using clear water until new roots grow out, and then transferring to conventional aerial fog growth culture.

Root cutting-rooting multiple times of cycles of aquilaria sinensis under the long-term aerosol culture condition, namely multiple times of stubble cutting and multiple times of rooting again. The method specifically comprises the following steps: cutting the regenerated aerosol root on the main root from the root base; spraying growth regulator solution with lower concentration on the main roots for inducing and re-rooting, wherein the growth regulator solution is commercial ABT rooting powder; after the rooting inducer liquid film on the surface of the main root is slightly dried, carrying out aerial fog culture by using clear water until adventitious roots can be seen by naked eyes to form, and then carrying out aerial fog root growth culture by using a normal-concentration conventional nutrient solution; before the aerial fog roots can be seen to regenerate by naked eyes, special attention is paid to reducing the transpiration of the overground part (such as spraying a transpiration inhibitor, regularly spraying water, covering and moisturizing).

In this example, the aeroponic facility utilized a simple transfer box retrofit, a domestic water purification pump, fixed spray intervals, a general nutrient solution formulation for the japanese pilot plant, 10 seedlings supplied with 1 liter of water or solution, liquid changes once every 7 days (during which no pH and EC changes were detected), and indoor light, temperature, and humidity environmental conditions in the laboratory. It can be understood that, based on the same principle, the aerosol facility, construction and management means can be changed according to the production scale and the input cost, thereby realizing more scientific and efficient practical effects: such as using an integral modern greenhouse, three-dimensional planting, developing a specific computer expert system, real-time detection and intelligent automatic control, artificial supplementary lighting, carbon dioxide gas fertilizer dressing, various measures for eliminating microorganism breeding, such as ultraviolet irradiation and the like.

(3) Induction of perfume substance in aquilaria sinensis isolated root

Immediately attaching agilawood inducer (shown in figure 1) to the surface of fresh aerial fog roots after each stubble cutting in a soaking or spraying mode, and timely stirring to prevent agglomeration during spreading and airing; slightly dried, and placed in LRH-100-GSI artificial climate incubator (Guangdong Tai Hongjun scientific instruments, Inc.) with humidity of 75%, darkness and normal temperature, wherein ultraviolet rays are irradiated for 10 minutes every 2 days to prevent microbial outbreak.

The dried aerosol after two weeks, as shown in fig. 2, can be subjected to detection of the characteristic components of lignum Aquilariae Resinatum and subsequent preparation of powder and extraction of essential oil.

The preparation method of the agilawood inducer (high-molecular functional membrane liquid) comprises the following steps: dispersing 2.1g of carrier beta-cyclodextrin (beta-CD) in 50mL of deionized water at 60 ℃ to obtain a beta-cyclodextrin carrier solution, adding 6mL of 40% agilawood induction factor ethephon solution into the beta-cyclodextrin carrier solution, performing water bath at 60 ℃ for 30min, adding 1g of carrier sodium carboxymethyl cellulose (CMC) into 1mL of absolute ethyl alcohol for dispersion, adding 80mL of deionized water while stirring to completely dissolve the carrier sodium carboxymethyl cellulose, then sequentially adding 0.5g of organic acid salicylic acid, 0.8g of glucose and 1.0g of inorganic salt sodium chloride, fixing the volume to 100mL, and performing volume division on the beta-cyclodextrin/ethephon solution and the sodium carboxymethyl cellulose solution at 500r/min according to the ratio of the volume of 1: 2 and mixing. Adding equal volume of the basement membrane ethylene-vinyl acetate copolymer emulsion into the solution at the temperature of 60 ℃ for 30min by water bath, fully stirring the mixture after 30min by water bath at the temperature of 60 ℃, standing the mixture at the temperature of 60 ℃ for 30min, removing bubbles on the surface of the mixture, and fully crosslinking and blending various substances in the solution.

After the aerial fog root is dried, the incense forming effect is checked by a simple incense method, the peculiar aroma is presented, an aquilaria sinensis aerial fog root sample is detected according to the agilawood standard of 2015 edition pharmacopoeia of the people's republic of China, the extract content meets the standard, and the incense forming marker substances 2- (2-phenethyl) chromone and sesquiterpene substances can be detected by a gas chromatography-mass spectrometry combined method, as shown in figures 3-5.

Example 2

The difference from example 1 is: the plant type is 1.5 years old, and the method of the agilawood inducer (high-molecular functional membrane liquid) is different as follows.

Dispersing 1.9g of carrier beta-cyclodextrin (beta-CD) in 50mL of deionized water at 60 ℃ to obtain a beta-cyclodextrin carrier solution, adding 1mL of agilawood induction factor methyl jasmonate into the beta-cyclodextrin carrier solution, stirring at room temperature and high speed in a homogenizer for 1 hour, adding 1.2g of base membrane sodium methyl cellulose (CMC) into 1mL of absolute ethyl alcohol for dispersion, adding 80mL of deionized water while stirring to completely dissolve the base membrane sodium methyl cellulose, then sequentially adding 0.3g of organic acid naphthylacetic acid, 0.5g of glucose and 1.2g of inorganic salt potassium chloride, fixing the volume to 100mL, and under 500r/min, mixing the beta-cyclodextrin/methyl jasmonate solution and the sodium methyl cellulose solution by the volume ratio of 1: 2 and mixing. Adding equal volume of the basement membrane ethylene-vinyl acetate copolymer emulsion into the solution at the temperature of 60 ℃ for 30min by water bath, fully stirring the mixture after 30min by water bath at the temperature of 60 ℃, standing the mixture at the temperature of 60 ℃ for 30min, removing bubbles on the surface of the mixture, and fully crosslinking and blending various substances in the solution.

Example 3

The difference from example 1 is: the plant type is 2.5 years old, and the method of the agilawood inducer (high-molecular functional membrane liquid) is different as follows.

Dispersing 2.1g of carrier beta-cyclodextrin (beta-CD) in 50mL of deionized water at 60 ℃ to obtain a beta-cyclodextrin carrier solution, adding 7mL of 40% agilawood induction factor ethylene solution into the beta-cyclodextrin carrier solution, and carrying out water bath at 60 ℃ for 30 min; adding 0.3g of organic acid indolebutyric acid, 1.0g of sucrose and 1.2g of inorganic salt sodium chloride into 20mL of deionized water while stirring; then, at 500r/min, mixing with beta-cyclodextrin/ethephon solution, adding 180mL of 10% basement membrane soluble chitosan solution, bathing for 30 minutes at 60 ℃ and fully stirring, then standing for 30 minutes at 60 ℃, removing bubbles on the surface of the solution, and fully crosslinking and blending various substances in the solution.

Example 4

Unlike example 1, the induction agent for lignum Aquilariae Resinatum was prepared according to the conventional method in the art.

Example 5

Unlike example 1, the induction factor for eaglewood is 6-benzylamino adenine.

It should be understood that the above description is of specific embodiments incorporating specific experimental conditions for further illustrating the invention and is not to be construed as limiting the practice of the invention to those descriptions. It will be apparent to those skilled in the art that various modifications, additions and substitutions (e.g., other edgeworthia species; e.g., modification of the nutrient solution for aerosol root culture and environmental parameters, etc.) can be made without departing from the spirit and scope of the invention (especially, the manner of harvesting aerosol root by stubble, the form and administration of the edgeworthia inducer) and are considered to be within the scope of the invention.

Based on the same principle, different adversity stimulation modes and parameters can be tried in the induction stage to obtain different incense forming effects, and various parameters can be optimized in the extraction and separation identification processes to obtain a better verification result.

The present invention is illustrated by the following examples, which are not intended to limit the scope of the invention. Other insubstantial modifications and adaptations of the present invention can be made without departing from the scope of the present invention.

Claims

1. A method for producing agilawood powder and essential oil by utilizing aquilaria sinensis aerial aeroponic radices is characterized by comprising the following steps:

(3) soaking or spraying in vitro aerial fog root with lignum Aquilariae Resinatum inducer to generate sesquiterpene and chromone compounds with lignum Aquilariae Resinatum marker in radix Aristolochice, and making into lignum Aquilariae Resinatum powder and essential oil;

the agilawood inducer in the step (3) is attached to the surface of the aerosol root in a soaking or spraying mode, the agilawood inducer comprises a basement membrane, agilawood induction factors, a carrier, inorganic salt, sugar and organic acid, the basement membrane comprises one or more of ethylene-vinyl acetate copolymer, cellulose derivatives, soluble chitosan and shellac, the agilawood induction factors comprise one or more of ethephon, 6-benzylaminopurine and methyl jasmonate, the carrier comprises one or more of cyclodextrin, ethyl cellulose and sodium carboxymethyl cellulose, the inorganic salt comprises one or two of sodium chloride and potassium chloride, the sugar comprises one or two of sucrose or glucose, and the organic acid comprises one or two of malic acid, citric acid and salicylic acid;

in the step (3), the isolated aerosol roots are soaked or sprayed by the agilawood inducer, and the isolated aerosol roots are kept standing in a cool and ventilated place to form a film on the surfaces of the isolated roots.

2. The method for producing agilawood powder and essential oil by using aquilaria sinensis aerial bacon according to claim 1, wherein the method comprises the following steps: the aquilaria sinensis seedling in the step (1) has a developed whole root system and a vertical main root, branches and a large crown, deep green leaves and a large number of seedlings, and the height of the seedling is lower than that of a conventional soil culture seedling; wherein, when the aquilaria sinensis seedlings are cultivated, special treatment is carried out, and the special treatment comprises that seeds are put in a paper tube to germinate and ensure that main roots are upright; and utilizing chlormequat chloride and/or topping and pruning to promote multiple branches to sprout from the main trunk without causing the branches to grow to be high in the seedling culture process, so that a large canopy width is formed, and the plant height, the canopy width, the branches and the leaf number are ensured.

3. The method for producing agilawood powder and essential oil by using aquilaria sinensis aerial bacon according to claim 1, wherein the method comprises the following steps: the aquilaria sinensis seedling meeting the requirements in the step (2) is characterized by comprising the following steps: the number of thin roots is large, the main root is upright and has a length of more than 15cm, the ground diameter is 0.5 +/-0.1 cm, the plant height is 50 +/-10 cm, more than 2 branches are provided, the number of leaves is large, the vigor is high, and the seedling age is 1.5-2.5 years.

4. The method for producing agilawood powder and essential oil by using aquilaria sinensis aerial bacon according to claim 1, wherein the method comprises the following steps: the process of continuously harvesting the regenerated aerial fog roots in the step (2) by adopting a multi-time stubble cutting mode comprises the following steps:

and (2.3) repeating the steps (2.1) - (2.2), and harvesting the regenerated aerosol roots for multiple times.

5. The method for producing agilawood powder and essential oil by using aquilaria sinensis aerial bacon according to claim 4, wherein the method comprises the following steps: the length of the aerosol roots regenerated in the steps (2.1) - (2.2) is 15-25 cm, and the rooting inducer in the step (2.2) is one or more of 6-benzylamino adenine, indolebutyric acid, naphthylacetic acid and commodity ABT rooting powder.

6. The method for producing agilawood powder and essential oil by using aquilaria sinensis aerial bacon according to claim 4, wherein the method comprises the following steps: in the step (2.2), the transpiration of the overground part needs to be reduced before the aerial fog roots visible to the naked eyes are regenerated and before the aerial fog roots are transferred to a nutrient solution for growth and culture, and measures comprise spraying a transpiration inhibitor on the leaves, spraying water on the leaves at regular intervals or relatively sealing the small plant growth environment.

7. The method for producing agilawood powder and essential oil by using aquilaria sinensis aerial bacon according to claim 1, wherein the method comprises the following steps: after the surface is filmed, the aerosol root is placed in a clean, breathable and dark environment, and the humidity of the environment is adjusted on the basis of the principle that the aerosol root is dehydrated to constant weight within 1-2 weeks.

8. The method for producing agilawood powder and essential oil by using aquilaria sinensis aerial fog bacon according to claim 1 or 7, wherein the method comprises the following steps: in the process of forming a film on the surface of the isolated aerosol root and generating the fragrant marked compounds of sesquiterpene and chromone substances in the aquilaria sinensis root, ozone, ultraviolet rays, hydrogen peroxide or potassium permanganate solution is adopted for disinfection treatment in order to prevent mildew.