CN111820873A - Living mouse muscle confocal imaging chamber - Google Patents
Living mouse muscle confocal imaging chamber Download PDFInfo
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- CN111820873A CN111820873A CN202010561001.9A CN202010561001A CN111820873A CN 111820873 A CN111820873 A CN 111820873A CN 202010561001 A CN202010561001 A CN 202010561001A CN 111820873 A CN111820873 A CN 111820873A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/0059—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/70—Means for positioning the patient in relation to the detecting, measuring or recording means
- A61B5/702—Posture restraints
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
- A61D3/00—Appliances for supporting or fettering animals for operative purposes
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Abstract
The invention relates to a living mouse muscle confocal imaging chamber which comprises a bottom plate, an imaging window arranged on the bottom plate, a frame arranged on the periphery of the bottom plate, a plurality of fixing piles oppositely arranged on the frame and elastic fixing belts arranged between the fixing piles, wherein the imaging window is arranged on the bottom plate; the experimental mouse is fixed between the elastic fixing band and the bottom plate. Compared with the prior art, the invention adopts the integrated imaging chamber, can enable the mouse to be completely laid on one side, and fixes the imaging parts of the trunk and the legs of the mouse through the rubber band, thereby achieving the best muscle confocal imaging effect.
Description
Technical Field
The invention belongs to the technical field of living body imaging devices, and relates to a living body mouse muscle confocal imaging chamber.
Background
In-vivo imaging is the highest requirement of scientific imaging, in-vivo muscle imaging is relatively easiest to implement, and the in-vivo muscle condition can be displayed most directly in real time. When the living body muscle of the mouse is imaged, the mouse needs to lie on the imaging chamber, the existing imaging chamber is mostly in a small round shape, the mouse cannot lie on the imaging chamber completely, and no fixing device is arranged, so that the imaging quality can be influenced.
Disclosure of Invention
The invention aims to overcome the defects of the prior art and provide an integrated living mouse muscle confocal imaging chamber, which is characterized in that a rubber band bypassing a bolt is used to stretch across the body or the rear leg of a mouse to enable the mouse to lie on the side and be fixed, so that the problems of difficult mouse living muscle confocal imaging and poor quality are solved.
The purpose of the invention can be realized by the following technical scheme:
a living mouse muscle confocal imaging chamber comprises a bottom plate, an imaging window arranged on the bottom plate, a frame arranged on the periphery of the bottom plate, a plurality of fixing piles oppositely arranged on the frame, and elastic fixing belts arranged between the fixing piles;
the experimental mouse is fixed between the elastic fixing band and the bottom plate.
Further, the bottom plate is a rectangular plate.
Further, the bottom plate is an acrylic plate.
Further, the imaging window includes an imaging aperture, a flange disposed radially inward at a bottom of a sidewall of the imaging aperture, and a cover glass mounted on the flange.
Further, the fixing piles are bolts fixedly arranged on the frame.
Furthermore, a plurality of spud piles are symmetrically arranged on two sides of the frame.
Preferably, the plurality of fixing piles are symmetrically arranged on 2 long sides of the rectangular frame.
Furthermore, 5 fixing piles are arranged on one side of the frame.
Furthermore, the elastic fixing band is a rubber band, two ends of each rubber band are respectively fixed on the fixing piles arranged on the 2 sides, and the experimental mouse is fixed between the rubber band and the bottom plate.
The working principle is as follows: during the experiment, earlier lie on the bottom plate with the mouse side for the experiment to make imaging positions such as mouse truck and shank aim at the formation of image window, later fix the rubber band with the mouse on the spud pile of locating 2 sides through both ends difference, thereby avoid rocking the relatively poor problem of living body muscle confocal imaging quality that leads to because of the mouse.
Compared with the prior art, the invention has the following characteristics:
1) compared with a conventional living cell and tissue imaging device which is matched with a microscope and has a small size, the invention integrally forms an imaging observation structure and a fixing device, can ensure that the head and the tail of a mouse can be laid at any left and right side by side through two imaging windows for placing cover glass, and compresses and fixes the imaging parts of the trunk and the rear legs of the mouse through rubber bands so as to achieve the optimal muscle confocal imaging effect;
2) the invention has simple structure, lower device cost, easy manufacture and wide raw material source, and the acrylic plates, bolts, metal frames and the like are all commercially available and easily purchased products.
Drawings
FIG. 1 is a schematic diagram of a confocal muscle imaging chamber of a living mouse according to the present invention;
the notation in the figure is:
1-frame, 2-bottom plate, 3-imaging window, 4-fixing pile.
Detailed Description
The invention is described in detail below with reference to the figures and specific embodiments. The present embodiment is implemented on the premise of the technical solution of the present invention, and a detailed implementation manner and a specific operation process are given, but the scope of the present invention is not limited to the following embodiments.
Example (b):
as shown in fig. 1, the living mouse muscle confocal imaging chamber includes a bottom plate 2, an imaging window 3 disposed on the bottom plate 2, a frame 1 disposed around the bottom plate 2, 10 fixing posts 4 symmetrically disposed on the frame 1, and an elastic fixing band disposed between the fixing posts 4.
The bottom plate 2 is a rectangular acrylic plate, the imaging window 3 comprises an imaging hole with the diameter of 26mm, a flange (the width of the flange is 1.75mm, the height of the flange is 1mm) which is arranged at the bottom of the side wall of the imaging hole inwards in the radial direction, and a cover glass with the diameter of 25mm which is erected on the flange; the spud pile 4 is the bolt of fixing on locating frame 1, and elastic fixation area is the rubber band.
The working principle is as follows: during the experiment, earlier lie on placing on bottom plate 2 with the mouse side for the experiment to make imaging positions such as mouse truck and shank aim at imaging window 3, fix the mouse through both ends respectively on fixed pile 4 of locating 2 sides, thereby avoid rocking the relatively poor problem of living body muscle confocal imaging quality that leads to because of the mouse.
The embodiments described above are described to facilitate an understanding and use of the invention by those skilled in the art. It will be readily apparent to those skilled in the art that various modifications to these embodiments may be made, and the generic principles described herein may be applied to other embodiments without the use of the inventive faculty. Therefore, the present invention is not limited to the above embodiments, and those skilled in the art should make improvements and modifications within the scope of the present invention based on the disclosure of the present invention.
Claims (8)
1. A confocal imaging cell for muscles of a living mouse is characterized by comprising a bottom plate (2), an imaging window (3) arranged on the bottom plate (2), a frame (1) arranged on the periphery of the bottom plate (2), a plurality of fixing piles (4) oppositely arranged on the frame (1), and elastic fixing bands arranged between the fixing piles (4);
the experimental mouse is fixed between the elastic fixing band and the bottom plate (2).
2. A living mouse confocal muscle imaging chamber as claimed in claim 1, characterized in that said base plate (2) is a rectangular plate.
3. A living mouse confocal muscle imaging chamber as claimed in claim 1, characterized in that said base plate (2) is an acrylic plate.
4. A living mouse confocal muscle imaging chamber as claimed in claim 1, wherein the imaging window (3) comprises an imaging hole, a flange provided radially inward at the bottom of the side wall of the imaging hole, and a cover glass mounted on the flange.
5. The small living mouse muscle confocal imaging chamber as claimed in claim 1, wherein the fixing posts (4) are bolts fixed on the frame (1).
6. The small living mouse muscle confocal imaging chamber as claimed in claim 1, wherein the plurality of fixing posts (4) are symmetrically arranged on two sides of the frame (1).
7. The small living mouse muscle confocal imaging chamber as claimed in claim 6, wherein 5 fixing piles (4) are arranged on one side of the frame (1).
8. The small living mouse confocal muscle imaging chamber as claimed in claim 1, wherein the elastic fixing band is a rubber band.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN2020102461332 | 2020-03-31 | ||
CN202010246133 | 2020-03-31 |
Publications (1)
Publication Number | Publication Date |
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CN111820873A true CN111820873A (en) | 2020-10-27 |
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CN202010561001.9A Pending CN111820873A (en) | 2020-03-31 | 2020-06-18 | Living mouse muscle confocal imaging chamber |
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Citations (14)
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CN201692094U (en) * | 2010-06-23 | 2011-01-05 | 德赛诊断***(上海)有限公司 | Living mouse experimental device |
CN202036227U (en) * | 2011-01-25 | 2011-11-16 | 同济大学 | Adjustable operating table used for measuring muscle strength of gastrocnemius and soleus of rat |
CN102599921A (en) * | 2011-01-25 | 2012-07-25 | 同济大学 | System for determining functions of lower limb nerves and muscles of rat |
CN203677088U (en) * | 2014-01-26 | 2014-07-02 | 四川大学华西医院 | Compatible fixing device of small animal formation of image |
CN203693823U (en) * | 2014-01-07 | 2014-07-09 | 上海中医药大学附属岳阳中西医结合医院 | Large and small mice fixing device |
CN204147139U (en) * | 2014-08-29 | 2015-02-11 | 上海中医药大学附属龙华医院 | A kind of rat fixing device |
CN207721918U (en) * | 2017-05-23 | 2018-08-14 | 苏州大学 | Mouse electro photoluminescence Simple fixing device |
CN108498204A (en) * | 2018-05-09 | 2018-09-07 | 首都医科大学宣武医院 | Animal spinal cord in-vivo imaging fixed observation device and use method thereof |
CN108606856A (en) * | 2018-05-28 | 2018-10-02 | 中国医学科学院生物医学工程研究所 | For the movable cerebral magnetic stimulation coil fixing device of mouse small range and method |
CN208552107U (en) * | 2017-09-13 | 2019-03-01 | 温州医科大学附属第一医院 | The automatic acupoint selection device of mouse model |
CN208838229U (en) * | 2018-05-09 | 2019-05-10 | 首都医科大学宣武医院 | Fixed viewing device of animal spinal cord live body formation of image |
CN209004307U (en) * | 2017-12-27 | 2019-06-21 | 南开大学 | A kind of fixation device of the living small animal window for being imaged under inverted fluorescence microscope |
CN110353720A (en) * | 2019-08-29 | 2019-10-22 | 中南大学湘雅医院 | Synchrotron radiation X-ray Micro-CT scanning and fixed device suitable for toy four limbs living imaging |
CN209751280U (en) * | 2018-10-31 | 2019-12-10 | 阜阳师范学院 | Device for fixing mouse in intragastric and abdominal cavity injection |
-
2020
- 2020-06-18 CN CN202010561001.9A patent/CN111820873A/en active Pending
Patent Citations (14)
Publication number | Priority date | Publication date | Assignee | Title |
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CN201692094U (en) * | 2010-06-23 | 2011-01-05 | 德赛诊断***(上海)有限公司 | Living mouse experimental device |
CN202036227U (en) * | 2011-01-25 | 2011-11-16 | 同济大学 | Adjustable operating table used for measuring muscle strength of gastrocnemius and soleus of rat |
CN102599921A (en) * | 2011-01-25 | 2012-07-25 | 同济大学 | System for determining functions of lower limb nerves and muscles of rat |
CN203693823U (en) * | 2014-01-07 | 2014-07-09 | 上海中医药大学附属岳阳中西医结合医院 | Large and small mice fixing device |
CN203677088U (en) * | 2014-01-26 | 2014-07-02 | 四川大学华西医院 | Compatible fixing device of small animal formation of image |
CN204147139U (en) * | 2014-08-29 | 2015-02-11 | 上海中医药大学附属龙华医院 | A kind of rat fixing device |
CN207721918U (en) * | 2017-05-23 | 2018-08-14 | 苏州大学 | Mouse electro photoluminescence Simple fixing device |
CN208552107U (en) * | 2017-09-13 | 2019-03-01 | 温州医科大学附属第一医院 | The automatic acupoint selection device of mouse model |
CN209004307U (en) * | 2017-12-27 | 2019-06-21 | 南开大学 | A kind of fixation device of the living small animal window for being imaged under inverted fluorescence microscope |
CN108498204A (en) * | 2018-05-09 | 2018-09-07 | 首都医科大学宣武医院 | Animal spinal cord in-vivo imaging fixed observation device and use method thereof |
CN208838229U (en) * | 2018-05-09 | 2019-05-10 | 首都医科大学宣武医院 | Fixed viewing device of animal spinal cord live body formation of image |
CN108606856A (en) * | 2018-05-28 | 2018-10-02 | 中国医学科学院生物医学工程研究所 | For the movable cerebral magnetic stimulation coil fixing device of mouse small range and method |
CN209751280U (en) * | 2018-10-31 | 2019-12-10 | 阜阳师范学院 | Device for fixing mouse in intragastric and abdominal cavity injection |
CN110353720A (en) * | 2019-08-29 | 2019-10-22 | 中南大学湘雅医院 | Synchrotron radiation X-ray Micro-CT scanning and fixed device suitable for toy four limbs living imaging |
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