CN111778126A - Health wine for improving immunity and preparation method thereof - Google Patents

Health wine for improving immunity and preparation method thereof Download PDF

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CN111778126A
CN111778126A CN202010522056.9A CN202010522056A CN111778126A CN 111778126 A CN111778126 A CN 111778126A CN 202010522056 A CN202010522056 A CN 202010522056A CN 111778126 A CN111778126 A CN 111778126A
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raw materials
weight
fermentation
traditional chinese
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CN111778126B (en
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王圣军
李灿杨
蔡京菁
付李明
高满琴
徐平
高慧
彭仕梅
夏恒建
孙利伟
陈普生
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Lishizhen Pharmaceutical Group Co ltd
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Abstract

The invention discloses health care wine for improving immunity, which comprises rice grain, traditional Chinese medicine raw materials and fungi, wherein the weight ratio of the rice grain to the traditional Chinese medicine raw materials to the fungi is 15-130: 12.5 to 77: 0.2 to 10; the rice grain comprises the following raw materials in parts by weight: 10-100 parts of rice and 5-30 parts of coix seeds; the traditional Chinese medicine raw materials comprise the following raw materials in parts by weight: 1-8 parts of wild jujube, 1-8 parts of fructus alpiniae oxyphyllae, 2-10 parts of Chinese yam, 2-10 parts of rhizoma polygonati, 0.5-5 parts of radix puerariae, 1-8 parts of arillus longan, 2-10 parts of poria cocos, 2-10 parts of medlar and 1-8 parts of jujube; the fungus comprises the following raw materials in parts by weight: 0.1-5 parts of rhizopus and 0.1-5 parts of saccharomycetes. The invention adopts the traditional Chinese medicines according to a certain proportion, the traditional Chinese medicines are compatible and coordinated with each other, and the components have the function of enhancing immunity through mutual cooperation. The invention also provides a preparation method of the health wine.

Description

Health wine for improving immunity and preparation method thereof
Technical Field
The invention belongs to the technical field of deep processing of fermented wine, and particularly relates to health-care wine for improving immunity and a preparation method thereof.
Background
Yellow wine is one of the oldest wines in the world, is a unique wine variety in China, has a long history and enjoys the reputation of 'national wine' in the world. In the traditional yellow wine brewing, rice, husked millet, corn and wheat are mainly used as main raw materials, and the brewed wine is prepared by steaming, yeast adding, saccharification, fermentation, squeezing, filtering, wine decocting, storage and blending, generally has the alcohol content of 3-20%, belongs to low-alcohol brewed wine, is low in alcohol content, mellow in taste and unique in flavor, and is always popular with health-care and health-preserving people. The yellow wine has wide production area, various varieties and rich nutrition, and contains protein, carbohydrate, amino acid, vitamin, trace elements, various organic acids, ester substances and active ingredients.
With the development of economy and the improvement of the living standard of people, people pay more and more attention to the functions of food such as safety, nutrition, health care and the like, and the traditional yellow wine cannot meet the health care requirements of human bodies. Therefore, the development of novel health-care yellow wine by improving the formula and the process and adopting an innovative process to optimize fermentation is a hot trend of the current research. In the development of the novel health-care yellow wine, the innovation mainly comprises two aspects: on the one hand, the innovation of main raw materials is that other grain crops are added from the traditional glutinous rice, husked millet and the like, such as: grain crops such as corn, black rice, oat, potato, tartary buckwheat and the like; and health-care components with traditional Chinese medicine components such as kudzu root, rhodiola rosea, medlar, hawthorn, sea cucumber and the like are added; on the other hand, optimization of fermentation strains and optimization of fermentation conditions are essential. In recent years, although there are many health-care fermented wines, due to reasons of ingredients and production processes, raw materials are not fully utilized, and functional components depend on blending, so that the immunity-improving effect of the fermented wines is not ideal or is not a fermented wine in a strict sense.
Therefore, the development of the health care type fermented wine capable of improving the immunity has important practical significance for the development of novel health care yellow wine.
Disclosure of Invention
The invention aims to overcome the technical defects, and the first aspect of the invention aims to provide the health-care fermented wine for improving the immunity, which can enhance the immunity of a human body and can play good roles of strengthening physique, preserving health and protecting health after being drunk frequently; the invention aims to provide a preparation method of health care fermented wine for improving immunity, which has the advantages of short fermentation period, energy conservation and high grain utilization rate.
In order to achieve the technical purpose, the technical scheme provided by the invention provides health care wine for improving immunity, which comprises rice grain, traditional Chinese medicine raw materials and fungi, wherein the weight ratio of the rice grain to the traditional Chinese medicine raw materials to the fungi is 15-130: 12.5 to 77: 0.2-10, wherein:
the rice grain comprises the following raw materials in parts by weight: 10-100 parts of rice and 5-30 parts of coix seeds;
the traditional Chinese medicine raw materials comprise the following raw materials in parts by weight: 1-8 parts of wild jujube, 1-8 parts of fructus alpiniae oxyphyllae, 2-10 parts of Chinese yam, 2-10 parts of rhizoma polygonati, 0.5-5 parts of radix puerariae, 1-8 parts of arillus longan, 2-10 parts of poria cocos, 2-10 parts of medlar and 1-8 parts of jujube;
the fungus comprises the following raw materials in parts by weight: 0.1-5 parts of rhizopus and 0.1-5 parts of saccharomycetes.
The technical scheme of the invention also provides a preparation method of the health care wine, which comprises the following steps:
s1, preparing materials according to parts by weight;
s2, mixing the traditional Chinese medicine raw materials, extracting with ethanol, filtering after extraction to obtain an extracting solution and dregs, adding water into the dregs for extraction, filtering after extraction to obtain a filtrate, and concentrating the filtrate to obtain an extract;
s3, curing the rice grains, uniformly mixing the cured rice grains with rhizopus, the extract and water, and performing primary fermentation to obtain a primary fermentation product; uniformly mixing the pre-fermentation product, yeast and water, and performing main fermentation to obtain a main fermentation product; carrying out after-fermentation on the main fermentation product and the extracting solution to obtain an after-fermentation product; and carrying out subsequent treatment on the post-fermentation product to obtain a finished product of the health wine.
Compared with the prior art, the invention has the beneficial effects that:
1. according to the viewpoint of five-organ imbalance and essence, qi and blood loss caused by fatigue in traditional Chinese medicine, the coix seed, the wild jujube, the sharpleaf galangal fruit, the Chinese yam, the sealwort, the root of kudzu vine, the arillus longan, the poria cocos, the medlar and the jujube are adopted, wherein the coix seed is rice grain, can be used as a main raw material of health care wine, can also be used as a traditional Chinese medicine raw material, is a monarch drug, has the effects of clearing heat and promoting diuresis, strengthening spleen and stomach, strengthening tendons and bones and improving immunity, the four Chinese yam, the sealwort, the poria cocos and the medlar are ministerial drugs, can tonify spleen, lung and kidney, and has the effect of improving immunity, the four Chinese jujube, the sharpleaf galangal fruit, the arillus longan and the jujube are adjuvant drugs, have the effects of nourishing blood and tranquilization, and treating neurasthenia, the root of kudzu vine is a; the traditional Chinese medicine raw materials are relatively balanced and matched according to the principle of monarch, minister, assistant and guide, and are compatible and coordinated with each other, and the components coordinate with each other to regulate and nourish liver and kidney, nourish spleen and lung, improve physique and have the function of enhancing immunity; the traditional Chinese medicine raw materials provided by the invention belong to medicine and food dual-purpose substances, and are safe and reliable; the rice grain, the traditional Chinese medicine raw materials and the fungi are matched according to a certain proportion and are fermented in a front-middle-rear system, so that the active ingredients of the traditional Chinese medicine raw materials are involved in a microbial complex fermentation biochemical process in the whole process, the taste of the health-care wine is better, and the wine body is more coordinated; the health wine provided by the invention is suitable for sub-health people who are easy to fatigue, are susceptible and need to improve immunity;
2. the preparation method of the health wine provided by the invention does not need the traditional rice steaming process in the fermentation process, saves energy, and has stable and controllable process; the process of solid-state pre-fermentation and liquid-state main fermentation is combined, so that the effective ingredients in the traditional Chinese medicinal materials can be converted and utilized to the maximum extent, the fermentation efficiency is greatly improved, the fermentation period is shortened, the burden of environmental protection and decontamination is reduced, and the grain utilization rate is improved; and the health wine has more harmonious wine body and better taste, and is strictly fermented wine.
Drawings
FIG. 1 is a process flow chart for preparing the health wine of the invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is described in further detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
The embodiment of the invention provides health care wine for improving immunity, which comprises rice grain, traditional Chinese medicine raw materials and fungi, wherein the weight ratio of the rice grain to the traditional Chinese medicine raw materials to the fungi is 15-130: 12.5 to 77: 0.2-10, wherein:
the rice grain comprises the following raw materials in parts by weight: 10-100 parts of rice and 5-30 parts of coix seeds;
the traditional Chinese medicine raw materials comprise the following raw materials in parts by weight: 1-8 parts of wild jujube, 1-8 parts of fructus alpiniae oxyphyllae, 2-10 parts of Chinese yam, 2-10 parts of rhizoma polygonati, 0.5-5 parts of radix puerariae, 1-8 parts of arillus longan, 2-10 parts of poria cocos, 2-10 parts of medlar and 1-8 parts of jujube;
the fungus comprises the following raw materials in parts by weight: 0.1-5 parts of rhizopus and 0.1-5 parts of saccharomycetes.
The pharmacological mechanism of the invention is as follows:
coix seed: sweet, light, slightly cold and nontoxic; has effects of clearing heat, promoting diuresis, removing rheumatism, promoting urination, benefiting lung, expelling pus, invigorating spleen and stomach, and strengthening tendons and bones after entering spleen, stomach, lung and kidney channels. The coix seed has mild effect, is slightly cold without hurting the stomach, benefits the spleen without nourishing the greasy taste, and modern pharmacological research finds that a certain proportion of saturated fatty acid in the coix seed plays a main role in resisting cancer, and has the effects of inhibiting skeletal muscle contraction, relieving fever, easing pain, resisting inflammation, resisting tumor, enhancing immunologic function and the like; it also has mild effect in reducing blood sugar.
And (3) wild jujube: is prepared from Rhamnaceae plant, semen Ziziphi Spinosae is dry mature seed of Rhamnaceae plant, and fructus Ziziphi Spinosae is dry mature seed of Rhamnaceae plant. It is acid, mild, nontoxic, sweet in taste, mild in nature; has effects of nourishing liver, calming heart, tranquilizing mind, and arresting sweating, and can be used for treating neurasthenia, insomnia, dreaminess, night sweat, etc.
Fructus alpiniae oxyphyllae: pungent taste and warm nature; it has effects of warming spleen, stopping diarrhea, controlling salivation, warming kidney, reducing urination, and stopping nocturnal emission; can be used for treating spleen and stomach deficiency cold and sexual neurasthenia.
Chinese yam: sweet in taste and neutral in nature; it enters spleen, lung and kidney meridians. Has effects of invigorating qi, nourishing yin, invigorating spleen, lung and kidney, and stopping leukorrhagia. It can be used for treating spleen deficiency, anorexia, asthenia, loose stool, diarrhea, cough and asthma due to lung deficiency, spermatorrhea, leukorrhagia, frequent micturition, internal heat, and diabetes. Modern pharmacological research finds that the Chinese yam has the function of enhancing cellular immunity and humoral immunity.
Rhizoma polygonati: sweet in taste and neutral in nature; it has the functions of invigorating kidney essence, delaying senility, improving dizziness, soreness of the waist and knees, etc. and has certain curative effect. In addition, it can tonify yin of lung and kidney, and spleen, qi and spleen yin, tonify earth and promote the generation of gold, tonify the acquired body to nourish the innate body, and is indicated for chronic cough due to lung and kidney yin deficiency, spleen and stomach qi deficiency, lassitude, hypodynamia, anorexia, and weak pulse. Modern pharmacological research finds that: rhizoma Polygonati has effects in improving organism immunity, promoting synthesis of DNA, RNA and protein, and promoting lymphocyte transformation; has remarkable anti-tubercle bacillus effect; has inhibitory effect on various pathogenic fungi; it also has inhibitory effect on typhoid bacillus and staphylococcus aureus; has effects in increasing coronary blood flow, lowering blood pressure, reducing blood lipid, and relieving atherosclerosis of coronary artery; the preparation has obvious inhibition effect on hyperglycemia caused by epinephrine; also has adrenal cortex inhibiting and antiaging effects.
Kudzu root: sweet and pungent in flavor and cool in nature; it enters spleen and stomach meridians. Expelling pathogenic factors from muscles and skin, bringing down fever, promoting eruption, promoting the production of body fluid, quenching thirst, invigorating yang, and relieving diarrhea. It can be used for treating fever, stiffness and pain of neck and back, measles without adequate eruption, thirst due to fever, diabetes due to yin deficiency, dysentery due to heat-purging, and diarrhea due to spleen deficiency. The kudzuvine root contains abundant flavonoids, and the modern pharmacological research finds that the kudzuvine root total flavonoids can expand coronary vessels and cerebral vessels, increase coronary blood flow and cerebral blood flow, reduce myocardial oxygen consumption and increase oxygen supply. The kudzu root can directly dilate blood vessels to reduce peripheral resistance, has obvious blood pressure reducing effect and can better relieve the 'tightness' symptom of the hypertension patient. Puerarin can improve microcirculation, increase local blood flow, and inhibit platelet aggregation. Pueraria lobata has a wide beta-receptor blocking effect.
Longan pulp: sweet taste and warm nature; it enters heart and spleen meridians. Has effects of invigorating heart and spleen, nourishing blood, and tranquilizing mind. It can be used for treating excessive anxiety, overstrain heart and spleen, palpitation, insomnia, and amnesia. Modern pharmacological research finds that the longan pulp and gecko extracting solution can promote growth and strengthen physique. Can obviously prolong the normal-pressure hypoxia-resistant survival time of the mouse and reduce the death rate at low temperature.
Tuckahoe, poria cocos: sweet and light taste, mild in nature; it enters heart and lung. Has effects of inducing diuresis to alleviate edema, eliminating dampness, invigorating spleen, and calming heart. It can be used for treating edema, phlegm retention, diarrhea due to spleen deficiency, palpitation, and insomnia. Modern pharmacological researches find that the poria cocos-containing compound (codonopsis pilosula, bighead atractylodes rhizome and poria cocos) decoction can remarkably increase the natural rosette formation rate and the phytohemagglutinin-induced lymphocyte transformation rate and can also remarkably increase the serum IgG content after being orally taken, and the poria cocos has the effect of promoting cellular immunity and humoral immunity.
Medlar: mature fruit of Lycium barbarum L.of Solanaceae. Can nourish yin of liver and kidney, and treat hypopsia, cataract, dizziness, soreness of waist and knees, spermatorrhea, deafness, odontoseisis, premature gray hair, insomnia, dreaminess, yin deficiency of liver and kidney, hectic fever, night sweat, and diabetes due to deficiency of essence and blood. In addition, the medlar has the function of promoting immunity and regulating immunity; can increase testosterone level in blood, and has strengthening effect; promoting hematopoietic function; has obvious leukocyte increasing effect on normal healthy people; also has antiaging, mutation resisting, antitumor, blood lipid reducing, liver protecting, fatty liver resisting, blood glucose reducing, and blood pressure lowering effects.
Chinese date: sweet taste and warm nature; it enters spleen, stomach and heart meridians. Has effects of invigorating spleen, replenishing qi, nourishing blood, and tranquilizing. It is indicated for spleen deficiency syndrome, irritability and insomnia. The Chinese date can treat neurasthenia and vegetative nerve disorder, which belong to people with deficiency of qi and blood and malnutrition of heart-mind. The Chinese dates can enhance muscle strength and increase weight; can increase gastrointestinal mucus, correct gastrointestinal damage, and protect liver; has effects in increasing cAMP content in leukocyte and resisting allergy; has sedative and hypnotic effects; it also has effects in inhibiting cancer cell proliferation, resisting mutation, relieving pain, relieving cough, and eliminating phlegm.
According to the viewpoint of five-organ imbalance and essence, qi and blood loss caused by fatigue formation in traditional Chinese medicine, the health-care wine provided by the embodiment of the invention adopts semen coicis, wild jujube, fructus alpiniae oxyphyllae, Chinese yam, rhizoma polygonati, radix puerariae, arillus longan, poria cocos, medlar and jujube, wherein the semen coicis is rice grain, can be used as a main raw material of the health-care wine, can also be used as a traditional Chinese medicine raw material, is a monarch drug, has the effects of clearing heat and promoting diuresis, strengthening spleen and stomach, strengthening bones and muscles and improving immunity, is a ministerial drug, can tonify lung and kidney, and has the effect of improving immunity, and the four drugs of wild jujube, fructus alpiniae oxyphyllae, arillus longan and jujube are adjuvant drugs, have the effects of nourishing blood and soothing nerves, and treating neurasthenia, and the radix puerariae is a messenger drug, has abundant flavonoids, can expand coronary vessels and cerebral vessels; the traditional Chinese medicine raw materials are relatively and balanced in matching according to the principle of monarch, minister, assistant and guide, are compatible and coordinated with each other, and through mutual cooperation of the components, the traditional Chinese medicine composition has the effects of regulating and nourishing liver and kidney, nourishing spleen and lung, improving physique and enhancing immunity. Therefore, the health care wine provided by the embodiment of the invention has the function of enhancing the immunity of human bodies.
In some preferred embodiments of the invention, the weight ratio of the rhizopus to the yeast is 2-10: 1; by optimizing the proportion of the rhizopus to the yeast, the health care wine with better taste is obtained.
Fig. 1 is a process flow diagram for preparing a health wine for improving immunity according to an embodiment of the present invention, and as shown in fig. 1, the embodiment of the present invention provides a preparation method for a health wine for improving immunity, which includes the following steps:
(1) preparing materials according to parts by weight;
(2) mixing the Chinese medicinal materials, extracting with ethanol, filtering to obtain extractive solution and residue, extracting the residue with water, filtering to obtain filtrate, and concentrating the filtrate to obtain extract;
(3) after curing the rice grains, uniformly mixing the cured rice grains with rhizopus, the extract and water, and performing primary fermentation to obtain a primary fermentation product; uniformly mixing the pre-fermentation product, yeast and water, and performing main fermentation to obtain a main fermentation product; carrying out after-fermentation on the main fermentation product and the extracting solution to obtain an after-fermentation product; and carrying out subsequent treatment on the post-fermentation product to obtain a health-care wine finished product.
In some preferred embodiments of the present invention, when ethanol is used for extraction in step (2), the weight of ethanol is 5-20 times of the weight of the traditional Chinese medicine raw materials, and the concentration of ethanol is 30-95%.
In some preferred embodiments of the present invention, when the water is extracted in the step (2), the weight of the water is 5 to 20 times of the weight of the traditional Chinese medicine raw materials.
In the step (2), the filtrate is concentrated to 0.3-1 time of the weight of the traditional Chinese medicine raw materials, and the extract is obtained.
In the examples of the present invention, the manner of concentrating the filtrate is not limited, and for example: concentration under reduced pressure, membrane concentration, concentration under normal pressure or combination of these several concentration methods can be adopted, and those skilled in the art can select them according to the actual situation, and the examples of the present invention do not limit them further. The extraction process in the embodiment of the present invention is not limited, for example, the processes of soaking, decocting, refluxing, and percolating may be adopted, or a combination of these several extraction processes may be adopted, and those skilled in the art may select the extraction process according to actual situations, and the embodiment of the present invention is not further limited.
In some preferred embodiments of the present invention, the step (3) further comprises pulverizing and sieving the aged rice grains to obtain rice and coix seed powder with a particle size of 20-100 meshes, so as to facilitate better fermentation of the rice and coix seeds.
It should be noted that, in the embodiment of the present invention, the ripening method of the rice grain is not limited, for example: the rice can be fried, heated by microwave, puffed or the combination of the cooking modes, and only the rice and the coix seeds need to be cooked.
In some preferred embodiments of the present invention, the temperature of the pre-fermentation in step (3) is 25 to 38 ℃ and the fermentation time is 2 to 5 days.
In the embodiment of the present invention, when performing the pre-fermentation in step (3), a person skilled in the art can adjust the amount of water added according to actual conditions, for example, 5 times, 10 times or 20 times of water by weight of the cooked rice grains can be added; but in order to ferment the rhizopus and the rice grains at a proper concentration to obtain a finished product with good taste, the adding amount of the water is 3-6 times of the total weight of the rice and the coix seeds.
In some preferred embodiments of the present invention, the temperature of the main fermentation in step (3) is 26 to 37 ℃ and the fermentation time is 4 to 10 days.
In the embodiment of the present invention, during the main fermentation in step (3), a person skilled in the art may adjust the amount of water added according to actual conditions, for example, the amount of water added may be 100 times, 1000 times or even 10000 times of the weight of the yeast; however, in order to improve the fermentation efficiency and shorten the fermentation time, the addition amount of water is 1000-6000 times of the weight of the yeast.
In some preferred embodiments of the present invention, the temperature of the post-fermentation in step (3) is 8 to 30 ℃ and the fermentation time is 30 to 180 days.
In the embodiment of the present invention, when performing the after-fermentation in step (3), a person skilled in the art may adjust the addition amount of the water and the edible ethanol according to actual conditions, and adjust the alcohol concentration of the health wine to a suitable range, and the addition amount of the water and the edible ethanol in the present invention is not further limited.
In the embodiment of the present invention, the post-processing in step (3) specifically includes the following steps: and placing the post-fermentation product in a closed environment, ageing for 180-360 days at normal temperature, clarifying, precisely filtering, sterilizing and filling to obtain a health-care wine finished product.
In the embodiment of the invention, those skilled in the art can blend according to the style requirement, for example, rose or red date and other substances are added into the post-fermentation product to adjust the taste of the health wine, which is not further limited in the invention. The person skilled in the art can also add a preservative to prolong the storage time of the health wine, for example, a proper amount of a preservative such as sodium metabisulfite as a food additive is added to the post-fermentation product, and the person skilled in the art can add the preservative according to the actual needs, which is not further limited in the present invention.
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to the following embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
The experimental methods in the present invention are conventional methods unless otherwise specified. The experimental materials used in the present invention were all purchased from the market unless otherwise specified.
Example 1:
the embodiment 1 of the invention provides health care wine for improving immunity, which comprises rice grain, traditional Chinese medicine raw materials and fungi, wherein the weight ratio of the rice grain to the traditional Chinese medicine raw materials to the fungi is 65: 54: 4.5, wherein:
the rice grain comprises the following raw materials in parts by weight: 50 parts of rice and 15 parts of coix seeds;
the traditional Chinese medicine raw materials comprise the following raw materials in parts by weight: 5 parts of wild jujube, 5 parts of fructus alpiniae oxyphyllae, 8 parts of Chinese yam, 8 parts of rhizoma polygonati, 2 parts of radix puerariae, 5 parts of arillus longan, 8 parts of poria cocos, 8 parts of medlar and 5 parts of Chinese date;
the fungus comprises the following raw materials in parts by weight: 3.5 parts of rhizopus and 1 part of yeast.
The health wine for improving immunity is prepared by the following method:
(1) preparing materials according to parts by weight;
(2) mixing wild jujube, fructus alpiniae oxyphyllae, Chinese yam, rhizoma polygonati, radix puerariae, arillus longan, poria cocos, Chinese wolfberry and Chinese date to obtain traditional Chinese medicine raw materials, performing hot reflux extraction on the traditional Chinese medicine raw materials by using 40% ethanol 8 times of the weight of the traditional Chinese medicine raw materials, wherein the temperature of the hot reflux extraction is 90 ℃, the extraction time is 2 hours, filtering after extraction to obtain an extracting solution and dregs, storing the extracting solution for later use, adding water 8 times of the weight of the traditional Chinese medicine raw materials into the dregs to perform hot reflux extraction, performing the hot reflux extraction at the temperature of 100 ℃, the extraction time is 3 hours, filtering after extraction to obtain a filtrate, and concentrating the filtrate to 0.5 time of the weight of the traditional Chinese medicine raw materials;
(3) curing rice and coix seeds, crushing, sieving with a sieve of 20-40 meshes to obtain rice and coix seed powder, uniformly mixing the rice and coix seed powder with rhizopus, extract and water, wherein the addition amount of the water is 5 times of the total weight of the rice and coix seeds, and pre-fermenting the uniformly mixed raw materials at 37 ℃ for 3 days to obtain a pre-fermentation product; uniformly mixing the pre-fermentation product, yeast and water, wherein the adding amount of the water is 3000 times of the weight of the yeast, and performing main fermentation on the uniformly mixed material at 37 ℃ for 6 days to obtain a main fermentation product; uniformly mixing the main fermentation product and the extracting solution, and then performing after-fermentation at the temperature of 30 ℃, wherein the fermentation time is 60 days, so as to obtain an after-fermentation product; and (3) seasoning and blending the post-fermentation product, placing the post-fermentation product in a closed environment, ageing the post-fermentation product for 180-360 days at normal temperature, and clarifying, precisely filtering, sterilizing and filling the post-fermentation product to obtain a health-care wine finished product.
Example 2:
the embodiment 2 of the invention provides health care wine for improving immunity, which comprises rice grain, traditional Chinese medicine raw materials and fungi, wherein the weight ratio of the rice grain to the traditional Chinese medicine raw materials to the fungi is 15: 12.5: 3, wherein:
the rice grain comprises the following raw materials in parts by weight: 10 parts of rice and 5 parts of coix seeds;
the traditional Chinese medicine raw materials comprise the following raw materials in parts by weight: 1 part of wild jujube, 1 part of sharpleaf galangal fruit, 2 parts of Chinese yam, 2 parts of rhizoma polygonati, 0.5 part of kudzuvine root, 1 part of longan pulp, 2 parts of poria cocos, 2 parts of medlar and 1 part of Chinese date;
the fungus comprises the following raw materials in parts by weight: 2 parts of rhizopus and 1 part of yeast.
The health wine for improving immunity is prepared by the following method:
(1) preparing materials according to parts by weight;
(2) mixing wild jujube, fructus alpiniae oxyphyllae, Chinese yam, rhizoma polygonati, radix puerariae, arillus longan, poria cocos, Chinese wolfberry and Chinese date to obtain traditional Chinese medicine raw materials, performing hot reflux extraction on the traditional Chinese medicine raw materials by using 40% ethanol 6 times of the weight of the traditional Chinese medicine raw materials, wherein the temperature of the hot reflux extraction is 75 ℃, the extraction time is 3 hours, filtering after extraction to obtain an extracting solution and dregs, storing the extracting solution for later use, adding water 6 times of the weight of the traditional Chinese medicine raw materials into the dregs to perform hot reflux extraction, performing the hot reflux extraction at the temperature of 90 ℃, the extraction time is 4 hours, filtering after extraction to obtain a filtrate, and concentrating the filtrate to 0.3 time of the weight of the traditional Chinese medicine raw materials;
(3) curing rice and coix seeds, crushing, sieving with a sieve of 20-40 meshes to obtain rice and coix seed powder, uniformly mixing the rice and coix seed powder with rhizopus, extract and water, wherein the addition amount of the water is 3 times of the total weight of the rice and coix seeds, and pre-fermenting the uniformly mixed raw materials at 25 ℃ for 5 days to obtain a pre-fermentation product; uniformly mixing the pre-fermentation product, yeast and water, wherein the adding amount of the water is 1000 times of the weight of the yeast, and performing main fermentation on the uniformly mixed material at 26 ℃ for 7 days to obtain a main fermentation product; uniformly mixing the main fermentation product and the extracting solution, and then performing after-fermentation at 26 ℃ for 90 days to obtain an after-fermentation product; and (3) seasoning and blending the post-fermentation product, placing the post-fermentation product in a closed environment, ageing the post-fermentation product for 180-360 days at normal temperature, and clarifying, precisely filtering, sterilizing and filling the post-fermentation product to obtain a health-care wine finished product.
Example 3:
the embodiment 3 of the invention provides health care wine for improving immunity, which comprises rice food, traditional Chinese medicine raw materials and fungi, wherein the weight ratio of the rice food to the traditional Chinese medicine raw materials to the fungi is 130: 77: 6, wherein:
the rice grain comprises the following raw materials in parts by weight: 100 parts of rice and 30 parts of coix seeds;
the traditional Chinese medicine raw materials comprise the following raw materials in parts by weight: 8 parts of wild jujube, 8 parts of sharpleaf galangal fruit, 10 parts of Chinese yam, 10 parts of rhizoma polygonati, 5 parts of kudzu root, 8 parts of longan pulp, 10 parts of poria cocos, 10 parts of medlar and 8 parts of Chinese date;
the fungus comprises the following raw materials in parts by weight: 5 parts of rhizopus and 1 part of yeast.
The health wine for improving immunity is prepared by the following method:
(1) preparing materials according to parts by weight;
(2) mixing wild jujube, fructus alpiniae oxyphyllae, Chinese yam, rhizoma polygonati, radix puerariae, arillus longan, poria cocos, Chinese wolfberry and Chinese date to obtain traditional Chinese medicine raw materials, performing hot reflux extraction on the traditional Chinese medicine raw materials by using 40% ethanol with the weight being 10 times of that of the traditional Chinese medicine raw materials, wherein the temperature of the hot reflux extraction is 85 ℃, the extraction time is 1 hour, filtering after extraction to obtain an extracting solution and dregs, storing the extracting solution for later use, adding water with the weight being 10 times of that of the traditional Chinese medicine raw materials into the dregs to perform hot reflux extraction, the temperature of the hot reflux extraction is 100 ℃, the extraction time is 2 hours, filtering after extraction to obtain a filtrate, concentrating the filtrate to 1 time of the weight of the traditional;
(3) curing rice and coix seeds, crushing, sieving with a 20-40 mesh sieve to obtain rice and coix seed powder, uniformly mixing the rice and coix seed powder with rhizopus, extract and water, wherein the addition amount of the water is 6 times of the total weight of the rice and coix seeds, and pre-fermenting the uniformly mixed raw materials at 38 ℃ for 2 days to obtain a pre-fermentation product; uniformly mixing the pre-fermentation product, yeast and water, wherein the adding amount of the water is 6000 times of the weight of the yeast, and performing main fermentation on the uniformly mixed material at 37 ℃ for 5 days to obtain a main fermentation product; uniformly mixing the main fermentation product and the extracting solution, and then performing after-fermentation at the temperature of 20 ℃, wherein the fermentation time is 30 days, so as to obtain an after-fermentation product; and (3) seasoning and blending the post-fermentation product, placing the post-fermentation product in a closed environment, ageing the post-fermentation product for 180-360 days at normal temperature, and clarifying, precisely filtering, sterilizing and filling the post-fermentation product to obtain a health-care wine finished product.
Test example 1:
the health-care wine is proved to be helpful for enhancing immunity by adopting the following method:
1. the material and the method are as follows:
(1) test samples: the health wine in the embodiment 1 is qualified and meets the technical requirements after inspection;
(2) test animals: 80 SPF-grade CI/F1 generation healthy female mice with the weight of 18.0-21.8 g. Mice were randomized into two groups of 4 doses each, 10 per dose, by body weight. Wherein the I group of mice are subjected to antibody-producing cell detection and half hemolysis value HC 50 determination; group II mice were tested for NK cell activity.
(3) Dose selection and subject administration regimen: the recommended dosage of the human body of the test sample is 100 g/human body (weight is 70 kg) per day, and the alcohol content of the finished product is 20 degrees. This laboratory used a method of concentration under reduced pressure (temperature 60 ℃ C.), concentrated test samples as test samples (20:1) of this experiment, and the human recommended amount of the test samples after the concentration was 0.07g/kg · bw/d (body weight 70 kg). The equivalent dose of the mice is 0.65g/kg according to the requirements of the pharmacological test method. According to the requirements of 'health food inspection and evaluation technical specifications', low dose groups, medium dose groups and high dose groups are respectively arranged, the low dose groups, the medium dose groups and the high dose groups are respectively administrated in 5 times, 10 times and 20 times, namely 3.25 g/kg-bw/d, 6.5 g/kg-bw/d and 13 g/kg-bw/d, a physiological saline negative control group is additionally arranged, and various immunity enhancing function indexes are measured after continuous intragastric administration for one month.
(4) Instruments and reagents: the device comprises a puncher, a T1000 type electronic balance, a JA2003 type electronic balance, a SG-603 biological safety cabinet, a multifunctional microplate reader, a carbon dioxide incubator and a JJ-100 electronic balance; SRBC, RPMI1640, YAC-1 cells.
2. The test method comprises the following steps:
(1) antibody-producing cell assay-Jerne modified slide method: each dose group of animals was continuously gavaged for one monthThen, each mouse was immunized by intraperitoneal injection of 0.2mL of 2% (v/v) SRBC suspension, 4 days later, the mouse was sacrificed by dislocation of cervical vertebrae, the spleen was taken out, placed in a small plate containing a suitable amount of sterile Hank's solution, the spleen was ground to prepare a cell suspension, filtered through a200 mesh screen, centrifuged (1000r/min) for 10min, washed 2 times with Hank's solution, and finally suspended in 5mLRPMI1640 medium to count the number of cells, and the cell concentration was adjusted to 5 × 10 using RP1640 MI medium6Heating and dissolving a surface layer culture medium, placing the surface layer culture medium in a 45 ℃ water bath for heat preservation, mixing the surface layer culture medium with an equivalent amount of Hank's solution with the concentration of 7.2-7.4 times, subpackaging the mixture into small test tubes, wherein each tube is 0.5mL, adding 10% SRBC (v/v prepared by SA buffer solution) 50 mu L and 20 mu L of spleen cell suspension (5 × 106/mL) into the tubes, quickly mixing the mixture uniformly, pouring the mixture onto a glass slide with a brushed agarose thin layer to form two parallel sheets, horizontally buckling the glass slide on a slide rack after the agar is solidified, placing the glass slide on a 37 ℃ rack, and placing the glass slide on a 5% CO rack at the temperature of 37 DEG C2And (3) incubating in an incubator for 1.5h, diluting the prepared complement by 1:8, adding the diluted complement into a groove of a slide frame, and counting the number of hemolytic plaques after continuing incubating for 1.5 h.
Number of hemolytic plaques ═ number of hemolytic plaques × 10
By number of plaques/106The number of plaques in the test sample group is significantly higher than that in the control group, as shown by spleen cells, and the test result can be judged to be positive.
(2) Serum hemolysin assay-half maximal hemolysin value (HC)50): after the animals of each dose group are continuously gavaged for one month, 2% (v/v) SRBC suspension is prepared, 0.2mL of the SRBC suspension is injected into the abdominal cavity of each mouse for immunization, after 4 days, blood is taken from the eyeground venous plexus of the mouse in a centrifugal tube, the centrifugal tube is placed for 1h, 2000r/min is used for centrifugation for 10min, and serum is separated and collected. After 200-fold dilution of serum, the optical density values of the sample tube and the SRBC at half hemolysis were determined according to the test method. The amount of hemolysin is expressed as half the hemolysin value (HC)50) And (4) showing.
HC50(sample photometric value/optical density value at half SRBC hemolysis) dilution factor
The amount of hemolysin is expressed as half the hemolysin value (HC)50) Indicates HC of the test sample group50HC significantly higher than control group50The test result can be judged to be positive.
(3) NK cell Activity determination-lactate dehydrogenase assay method, wherein after animals of various dose groups are continuously gazed for one month, mice are killed by dislocation of cervical vertebrae, spleens are taken aseptically, the mice are placed in a small plate containing a proper amount of aseptic Hank's solution, the spleens are ground to prepare single cell suspension, the single cell suspension is filtered by a 200-mesh screen, the suspension is washed by the Hank's solution for 2 times, the suspension is centrifuged for 10min (1000r/min) each time, cell pulp is bounced by supernatant, 0.5mL of sterile water is added for 20 seconds, 0.5mL of 2-fold Hank's solution and 8mL of Hank's solution are added after red blood cells are lysed, the suspension is centrifuged for 10min (1000r/min), 10% of calf serum RPMI1640 complete culture solution is used for resuspension, 1% glacial acetic acid is used for dilution, the number of viable cells is counted by staining of taloprene blue (all over 95%), the concentration is adjusted to 2 × 10 by7one/mL.
Subculturing target cells (YAC-1 cells) 24h before experiment, washing with Hank's solution 3 times before application, and adjusting cell concentration to 4 × 10 with RPMI1640 complete culture solution5one/mL. Adding YAC-1 cells and splenocytes 100 μ L (effective target ratio 50:1) into U-shaped 96-well culture plate, adding YAC-1 cells and culture solution 100 μ L into YAC-1 cell natural release hole, adding YAC-1 cells and culture solution 100 μ L into YAC-1 cell maximum release hole, adding YAC-1 cells and 2.5% Triton 100 μ L, respectively, providing three parallel holes, and culturing at 37 deg.C and 5% CO2Culturing for 4h in an incubator, centrifuging the 96-well culture plate for 5min at 1500r/min, sucking 100 mu L of supernatant per well, placing the supernatant in a flat-bottomed 96-well culture plate, adding 100 mu L of LDH matrix solution, reacting for 10min, adding 30 mu L of 1mol/L HCl per well, and measuring the optical density value at 490nm of a microplate reader.
NK cell activity (%) - (reaction well OD-natural release well OD)/(maximum release well OD-natural release well OD) × 100%
The NK cell activity of the tested sample group is obviously higher than that of the control group, so that the test result can be judged to be positive.
(4) And (3) test data statistics: carrying out the homogeneity test of variance on each test original data by SPSS10.0 software, carrying out statistical treatment on data meeting the variance requirement by using a pairwise comparison method of mean values between a plurality of test groups and a control group in a single-factor variance analysis method; and carrying out proper variable conversion on data with non-normal distribution or uneven variance, and carrying out statistical processing on data obtained by conversion after meeting the requirements of normal distribution or uniform variance.
(5) In the test process, the animals have normal drinking water and normal ingestion and no abnormal appearance.
3. And (3) test results:
(1) effect of health wine on mouse body weight: the results are shown in Table 1.
As can be seen from Table 1, the difference between the initial weight groups of the low, medium and high dose mice and the negative control group is not statistically significant (P > 0.05), indicating that the initial weight of the mice is more balanced among the groups.
After one month of the health-care wine with different doses for gastric perfusion, the final weight and the growth value of each dose group are subjected to the homogeneous variance test to meet the homogeneous variance requirement, the statistical treatment is carried out by a pairwise comparison method of the mean number between a plurality of test groups and a control group in the single-factor variance analysis method, and the difference has no statistical significance (P & gt 0.05).
TABLE 1 Effect of health wine on mouse body weight
Figure BDA0002532497430000121
(2) The health wine has effects on thymus and spleen organs of mice: the results are shown in Table 2.
After the mice are fed with hair health care wine with different dosages through gastric lavage for one month, weighing the thymus and the spleen of the mice, calculating the visceral volume ratio, carrying out the homogeneity test of variance on the visceral volume ratio to meet the requirement of homogeneity of variance, and carrying out statistical treatment by using a pairwise comparison method of mean between a plurality of test groups and a control group in a single-factor variance analysis method. As can be seen from the results in Table 2, the differences between the test group and the negative control group have no statistical significance (P > 0.05).
TABLE 2 influence of health wine on thymus and spleen organs of mice
Figure BDA0002532497430000122
(3) Effect of health wine on mouse antibody-producing cells (number of hemolytic plaques): the results are shown in Table 3.
After a month of administration of different doses of health care wine to a mouse by gavage, a mouse antibody generation cell experiment is carried out by a Jerne improved glass slide method, the number of hemolytic plaques is calculated, the homogeneity of variance is tested to meet the requirement of homogeneity of variance, and statistical treatment is carried out by a pairwise comparison method of mean numbers between a plurality of test groups and a control group in a single-factor variance analysis method. As can be seen from the results in Table 3, the number of the hemolytic plaques in the medium and high dose groups was higher than that in the negative control group, and the difference was statistically significant (P < 0.05).
TABLE 3 Effect of health wine on the number of hemolytic plaques in mice
Figure BDA0002532497430000131
(4) The health wine has half haemolysis value (HC) to mouse serum50) The influence of (a): the results are shown in Table 4.
After one month of administration of various doses of fermented liquor to mice by gavage, the half haemolysis value (HC) of serum of the mice was measured by the half haemolysis value method50) And carrying out the homogeneity test of the variance to meet the requirement of the homogeneity of the variance, and carrying out statistical treatment by using a pairwise comparison method of mean values between a plurality of test groups and a control group in a single-factor variance analysis method. As can be seen from the results in Table 4, the medium and high dose groups showed half maximal Hemolysis (HC) value in the mouse serum50) The height of the group is 0 mL/kg-bw/d, and the difference has statistical significance (P is less than 0.05).
TABLE 4 health wine vs. mouse HC50Influence of (2)
Figure BDA0002532497430000132
(5) Effect of health wine on mouse NK cell activity: the results are shown in Table 5.
After the mice are fed with the health care wine with different dosages through gastric lavage for one month, the activity of NK cells of the mice is measured by a lactate dehydrogenase measuring method, the activity of the NK cells is converted by sin-1P 1/2(P is the activity of the NK cells and is expressed by decimal), then the homogeneity test of variance is carried out, the requirement of the homogeneity of variance is met, and the statistical treatment is carried out by a pairwise comparison method of mean numbers between a plurality of test groups and a control group in a single-factor variance analysis method. As can be seen from the results in Table 5, the NK cell activity in the high dose test group was higher than that in the negative control group, and the difference was statistically significant (P < 0.05).
TABLE 5 Effect of health wine on NK cell Activity in mice
Figure BDA0002532497430000141
4. Conclusion
According to the technical specification for health food inspection and evaluation, the health wine provided in example 1 has positive results in any two aspects of cellular immune function (ConA-induced splenic lymphocyte transformation of mice, DNFB-induced DTH of mice), humoral immune function (antibody-producing cell detection, serum hemolysin measurement), mononuclear-macrophage function (mouse carbon clearance, phagocytosis of chicken erythrocytes by mouse peritoneal macrophages) and NK cell activity, and the health wine sample provided in example 1 can be judged to have the function of enhancing immunity.
Wherein, it is required to be noted that: the two test results in the cellular immune function test items are positive, or the two dose groups in any test have positive results, so that the cellular immune function test result can be judged to be positive; the two test results in the humoral immunity function determination result are both positive, or the two dosage groups in any test result are positive, so that the positive humoral immunity function determination result can be judged; the result of two tests in the functional test items of the mononuclear-macrophage is positive, or the result of two dosage groups in any test is positive, so that the functional result of the mononuclear-macrophage can be judged to be positive; more than one dose group of the NK cell activity determination test has positive results, and the positive result of the NK cell activity can be judged.
The health wine provided by the invention is gavaged for one month in three doses of 3.25 g/kg-bw/d, 6.5 g/kg-bw/d and 13 g/kg-bw/d, and is used for measuring the humoral immune function and the NK cell activity of the mice. The results show that the health care wine has no influence on the weight of a mouse, and the thymus and spleen organs have no influence; the half hemolysis value and the hemolysis plaque number of the medium and high dose test groups are higher than those of the negative control group; the NK cell activity of the high-dose test group is higher than that of the negative control group, and the differences have statistical significance (P < 0.05). According to the result of the immunity enhancing function test of 'health food inspection and evaluation technical specification', the test sample health wine has the function of enhancing immunity.
The above-described embodiments of the present invention should not be construed as limiting the scope of the present invention. Any other corresponding changes and modifications made according to the technical idea of the present invention should be included in the protection scope of the claims of the present invention.

Claims (10)

1. The health wine for improving immunity is characterized by comprising rice grain, traditional Chinese medicine raw materials and fungi, wherein the weight ratio of the rice grain to the traditional Chinese medicine raw materials to the fungi is 15-130: 12.5 to 77: 0.2-10, wherein:
the rice grain comprises the following raw materials in parts by weight: 10-100 parts of rice and 5-30 parts of coix seeds;
the traditional Chinese medicine raw materials comprise the following raw materials in parts by weight: 1-8 parts of wild jujube, 1-8 parts of fructus alpiniae oxyphyllae, 2-10 parts of Chinese yam, 2-10 parts of rhizoma polygonati, 0.5-5 parts of radix puerariae, 1-8 parts of arillus longan, 2-10 parts of poria cocos, 2-10 parts of medlar and 1-8 parts of jujube;
the fungus comprises the following raw materials in parts by weight: 0.1-5 parts of rhizopus and 0.1-5 parts of saccharomycetes.
2. The health wine according to claim 1, wherein the weight ratio of the rhizopus to the yeast is 2-10: 1.
3. A method for preparing the health wine according to claim 1 or 2, comprising the steps of:
s1, preparing materials according to parts by weight;
s2, mixing the traditional Chinese medicine raw materials, extracting with ethanol, filtering after extraction to obtain an extracting solution and dregs, adding water into the dregs for extraction, filtering after extraction to obtain a filtrate, and concentrating the filtrate to obtain an extract;
s3, curing the rice grains, uniformly mixing the cured rice grains with rhizopus, the extract and water, and performing primary fermentation to obtain a primary fermentation product; uniformly mixing the pre-fermentation product, yeast and water, and performing main fermentation to obtain a main fermentation product; carrying out after-fermentation on the main fermentation product and the extracting solution to obtain an after-fermentation product; and carrying out subsequent treatment on the post-fermentation product to obtain a finished product of the health wine.
4. The preparation method of the health care wine according to claim 3, wherein when ethanol is used for extraction, the weight of the ethanol is 5-20 times of that of the traditional Chinese medicine raw materials, and the concentration of the ethanol is 30-95%.
5. The preparation method of the health care wine according to claim 3, wherein when water is added for extraction, the weight of the water is 5-20 times of that of the traditional Chinese medicine raw materials.
6. The preparation method of the health care wine according to claim 3, wherein the step S3 further comprises crushing and sieving the aged rice grains to obtain the rice and coix seed powder with the particle size of 20-100 meshes.
7. The method for preparing the health care wine according to claim 3, wherein the temperature of the pre-fermentation is 25-38 ℃, and the fermentation time is 2-5 days.
8. The preparation method of the health care wine according to claim 3, wherein the temperature of the main fermentation is 26-37 ℃, and the fermentation time is 4-10 days.
9. The preparation method of the health care wine according to claim 3, wherein the temperature of the after-fermentation is 8-30 ℃, and the fermentation time is 30-180 days.
10. The preparation method of the health care wine according to claim 3, wherein the subsequent treatment specifically comprises the following steps: and placing the post-fermentation product in a closed environment, ageing for 180-360 days at normal temperature, and then clarifying, precisely filtering, sterilizing and filling to obtain a health-care wine finished product.
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Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103859369A (en) * 2012-12-10 2014-06-18 天津红日药业股份有限公司 Health-preserving health food formula beneficial for beautifying and enlarging breasts and preparation method thereof
CN107841411A (en) * 2017-11-21 2018-03-27 桂林国农生态农业有限公司 A kind of processing method of health-care rice wine
CN108102844A (en) * 2018-01-22 2018-06-01 李时珍医药集团有限公司 A kind of health liquor and preparation method thereof
CN111184838A (en) * 2020-02-18 2020-05-22 南方医科大学 Traditional Chinese medicine composition containing coix seeds and application thereof

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103859369A (en) * 2012-12-10 2014-06-18 天津红日药业股份有限公司 Health-preserving health food formula beneficial for beautifying and enlarging breasts and preparation method thereof
CN107841411A (en) * 2017-11-21 2018-03-27 桂林国农生态农业有限公司 A kind of processing method of health-care rice wine
CN108102844A (en) * 2018-01-22 2018-06-01 李时珍医药集团有限公司 A kind of health liquor and preparation method thereof
CN111184838A (en) * 2020-02-18 2020-05-22 南方医科大学 Traditional Chinese medicine composition containing coix seeds and application thereof

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