CN111777662B - Active polypeptide, preparation method and application in anti-aging cosmetics - Google Patents
Active polypeptide, preparation method and application in anti-aging cosmetics Download PDFInfo
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- CN111777662B CN111777662B CN202010727176.2A CN202010727176A CN111777662B CN 111777662 B CN111777662 B CN 111777662B CN 202010727176 A CN202010727176 A CN 202010727176A CN 111777662 B CN111777662 B CN 111777662B
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- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
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Abstract
The invention discloses an active polypeptide, a preparation method and application in anti-aging cosmetics. Important manifestations of skin aging include: the skin's antioxidant capacity decreases, leading to aging; the collagen and elastin are gradually lost, and wrinkles and sagging occur. The invention provides an active polypeptide, which can improve the antioxidant capacity of human skin fibroblasts on one hand, and is probably related to the activity of catalase in the human skin fibroblasts improved by the active polypeptide; on the other hand, the content of type I collagen and elastin in human skin fibroblasts can be increased, so that skin wrinkles and sagging can be overcome. Therefore, the active polypeptide provided by the invention has the effect of resisting skin aging, and can be used as an anti-aging active component for preparing anti-aging cosmetics.
Description
Technical Field
The invention belongs to the field of cosmetics, and relates to an active polypeptide, a preparation method and application in anti-aging cosmetics.
Background
Anti-aging is always the focus of long-term attention in the cosmetic industry, and the aim of delaying skin aging is one of the pursuit targets of most people. Many people are keen in the research of anti-aging experiments and develop many anti-aging cosmetics.
Skin aging is a process of making the human body function or develop rapidly or slowly naturally under the influence of internal and external factors, mainly due to endogenous aging and exogenous aging. Endogenous aging is an irresistible complex natural law and mainly shows that cell metabolism is gradually slowed down, collagen is gradually lost, collagen is damaged, hormone secretion is disordered, fat content begins to be reduced, partial cells are naturally killed and the like, and the conditions that skin loses elasticity and luster, skin is dried to generate wrinkles, even more, melanin pigmentation, senile plaque generation and the like are reflected on the skin. Exogenous aging is mainly affected by environmental pollution, excessive ultraviolet irradiation and other factors. Skin aging is mainly gradually shown from an epidermal layer, an epidermis-dermis connecting layer and a dermis layer, for example, wrinkles on the skin are a main mark of skin aging, and the skin aging problems of wrinkles, looseness and the like are caused by loss of collagen and breakage of elastic fibers due to thinning of the skin thickness.
Currently, polypeptides are among the most popular anti-aging ingredients in anti-aging cosmetics. The study of polypeptides having anti-ageing activity is therefore also a endlessly variable.
Disclosure of Invention
The invention aims to provide an active polypeptide, a preparation method and application in anti-aging cosmetics.
The technical scheme of the invention is as follows:
an active polypeptide prepared by the steps of:
step S1, degreasing: pulverizing dried folium Artemisiae Argyi, and defatting with petroleum ether under reflux;
step S2, extracting: leaching degreased and solvent-volatilized folium Artemisiae Argyi with sodium hydroxide water solution with pH of 8.5-9.5 at room temperature, centrifuging the extractive solution at rotation speed of 4000-5000 r/min for 8-12 min;
step S3, decoloring: adding activated carbon into the centrifuged supernatant, stirring and decoloring, centrifuging and collecting the supernatant;
step S4, alcohol precipitation: adding absolute ethyl alcohol into the supernatant after decolorization and centrifugation to enable the final volume percentage concentration of the ethyl alcohol to be 60%, fully stirring, standing at a low temperature for 10-14 h, centrifuging at a rotating speed of 7500-8500 r/min for 15-25 min, collecting the supernatant, removing the ethyl alcohol through rotary evaporation, and freeze-drying to obtain a folium artemisiae argyi polypeptide crude extract;
step S5, gel column chromatography: dissolving folium Artemisiae Argyi polypeptide crude extract with distilled water, loading onto Sephadex G-25 gel chromatographic column, eluting with PBS buffer solution at flow rate of 1mL/min, monitoring with protein nucleic acid detector at wavelength of 280nm, collecting eluate corresponding to the 2 nd elution peak, and freeze drying.
Preferably, in step S1, the folium Artemisiae Argyi is degreased 1-3 times with petroleum ether of 8-12L volume for 1-2 h each time.
Preferably, in step S2, each kilogram of folium Artemisiae Argyi is leached at room temperature for 1-3 times, each time for 6-10 hours, with a volume of 6-10L of sodium hydroxide aqueous solution.
Preferably, in step S3, the amount of activated carbon is 0.2% w/v, the decolorizing temperature is 75 ℃, and the decolorizing time is 30 min.
Preferably, in step S4, the low temperature means 4 ℃.
Preferably, in step S5, the Sephadex G-25 gel chromatography column has an inner diameter and a bed height of 1: 10.
The active polypeptide is used for improving the antioxidant capacity of skin.
The use of the above active polypeptide for increasing the collagen content in skin.
The use of the above active polypeptide for increasing elastin content in skin.
The application of the active polypeptide in preparing anti-aging cosmetics.
The beneficial technical effects are as follows:
the invention provides a polypeptide which is separated from folium artemisiae argyi. On one hand, the active polypeptide can improve the antioxidant capacity of the human skin fibroblasts, which is probably related to that the active polypeptide can improve the activity of catalase in the human skin fibroblasts; on the other hand, the content of type I collagen and elastin in human skin fibroblasts can be increased, so that skin wrinkles and sagging can be overcome. Therefore, the active polypeptide provided by the invention has the effect of resisting skin aging, and can be used as an anti-aging active ingredient for preparing anti-aging cosmetics.
Drawings
FIG. 1 is a chromatogram for monitoring the elution of a gel column;
FIG. 2 is a comparison of human skin fibroblast proliferation activity in each group;
FIG. 3 is a comparison of catalase activity in human skin fibroblasts of various groups;
FIG. 4 is a comparison of the content of type I collagen and elastin in human skin fibroblasts of each group.
Detailed Description
The following examples are intended to illustrate the essence of the present invention, but should not be construed as limiting the scope of the present invention.
Example 1: preparation of active polypeptide
First, test materials
The raw material is dry folium Artemisiae Argyi of Artemisia of Compositae.
The active carbon is conventional active carbon powder for decolorization, and the chemical reagents are conventional reagents.
Second, method and results
The preparation steps are as follows:
step S1, degreasing: pulverizing dried folium Artemisiae Argyi, defatting with petroleum ether under reflux, defatting with 10L petroleum ether for 2 times per kg folium Artemisiae Argyi, each time for 1.5 hr;
step S2, extracting: extracting defatted folium Artemisiae Argyi with pH 9.0 sodium hydroxide aqueous solution at room temperature, centrifuging the extractive solution at 4500r/min for 10 min; wherein, each kilogram of folium Artemisiae Argyi is leached at room temperature for 2 times, each time for 8h, with 8L volume of sodium hydroxide aqueous solution;
step S3, decoloring: adding activated carbon into the centrifuged supernatant, stirring and decoloring, centrifuging and collecting the supernatant; wherein the dosage of the active carbon is 0.2% w/v, the decoloring temperature is 75 ℃, and the decoloring time is 30 min;
step S4, alcohol precipitation: adding anhydrous ethanol into the supernatant after decolorization and centrifugation to make the final volume percentage concentration of ethanol be 60%, fully stirring, standing at 4 ℃ for 12h, centrifuging at the rotating speed of 8000r/min for 20min, collecting the supernatant, performing rotary evaporation at 50 ℃ to remove ethanol, and freeze-drying to obtain folium Artemisiae Argyi polypeptide crude extract;
step S5, gel column chromatography: dissolving folium Artemisiae Argyi polypeptide crude extract with distilled water (10mg/mL), loading onto Sephadex G-25 gel chromatographic column (loading volume is 1/10 of column bed volume) with inner diameter and column bed height ratio of 1:10, eluting with PBS buffer solution at flow rate of 1mL/min, monitoring with protein nucleic acid detector at wavelength of 280nm, and FIG. 1 is gel column chromatography elution monitoring chromatogram, collecting eluate corresponding to the 2 nd elution peak, and freeze drying.
Example 2: anti-aging Activity of active Polypeptides
First, test materials
Active polypeptide was prepared as in example 1 and stored at-20 ℃ until use.
Human skin fibroblasts (HDFs) were purchased from zermer fly, were cryopreserved HDF-a cells, normal human skin fibroblasts from adult skin, and were cryopreserved at the end of primary culture.
Calf serum, DMEM medium, penicillin and streptomycin were purchased from Gibco, USA.
Human Catalase (CAT) enzyme-linked immunoassay kit was purchased from Biyuntian biotechnology Co.
Second, test method
1. Cell culture
HDF cells were cultured in DMEM containing 10% calf serum, 1% penicillin and 1% streptomycin at 37 ℃ with 5% CO2Culturing under the condition of relative saturation humidity, carrying out passage for 2-3 d, and taking cells in logarithmic growth phase for testing.
2. MTT method for measuring cell proliferation activity
Taking HDF cells in logarithmic growth phase, adjusting cell density to 5 × 104one/mL, inoculated in a 96-well plate,200 mu L of the extract is cultured for 24h, and then randomly divided into a normal control group, an oxidative damage group and a polypeptide protection group. The polypeptide protection group is continuously cultured by using complete culture medium containing active polypeptide (5, 10 and 20 mu g/mL) with different final mass concentrations, the normal control group and the oxidative damage group are continuously cultured by using normal complete culture medium, and after 24 hours, the oxidative damage group and the polypeptide protection group are replaced by the medium containing 150 mu M H2O2The complete medium was cultured for 12 hours. Discarding the culture solution, washing with PBS, adding 100 μ L MTT solution with concentration of 0.5mg/L into each well, incubating for 4h, carefully absorbing the liquid in each well, adding 150 μ L DMMSO into each well, oscillating for 10min, measuring the light absorption value A of each well under the 490nm wavelength of an enzyme labeling instrument, and calculating the cell proliferation activity according to the formula. And 5 multiple wells are respectively arranged at each concentration of the normal control group, the oxidative damage group and the polypeptide protection group. Cell proliferation activity is x 100% in the a oxidative damage group or the polypeptide protective group/a normal control group.
3. Determination of catalase activity by kit
Setting control group and polypeptide group, culturing the polypeptide group with complete culture medium containing active polypeptide (10, 20 μ g/mL) with different final mass concentration, culturing the control group with normal complete culture medium, centrifuging after 72h, discarding supernatant, washing with PBS, and diluting to 1 × 106cell/mL cell solution, repeated freeze thawing to prepare cell pulp, centrifuging to obtain cell supernatant, and determining catalase activity according to the specification of a human Catalase (CAT) enzyme-linked immunoassay kit, and performing parallel experiments for 3 times.
4. Western blot method for determining content of type I Collagen (Collagen I) and elastin (elastin)
Setting control group and polypeptide group, culturing the polypeptide group with complete culture medium containing active polypeptide (10, 20 μ g/mL) with different final mass concentration, culturing the control group with normal complete culture medium, centrifuging after 72h, discarding supernatant, washing with PBS, and diluting to 1 × 106Freezing and thawing the cell solution/mL repeatedly to prepare cell plasma, centrifuging to obtain cell supernatant, determining protein concentration by BCA method, performing SDS-PAGE electrophoresis on equal amount of protein, concentrating gel at 80V for 30min, separating gel for 90min, wet transferring the gel to PVDF membrane, sealing 5% skimmed milk at room temperature for 2h, incubating at 4 deg.C for resisting Collagen I, Elastin and GAPDH overnightAnd (3) washing the membrane by PBST for 3 times, wherein each time is about 15min, incubating the corresponding secondary antibody at normal temperature for 1.5h, washing the membrane by PBST for 3 times, and each time is 15min, developing the PVDF membrane by adopting Biyuntian ECL (electron cyclotron resonance) luminous liquid by using a luminometer, and photographing and analyzing.
5. Data processing
All data were processed using SPSS15.0 software, expressed as mean ± s, and comparisons between groups were by one-way anova, with P < 0.05 indicating significant differences.
Third, test results
1. Effect of active polypeptide on human skin fibroblast oxidative damage resistance
The cell proliferation activity of each group is shown in table 1 and fig. 2, and compared with a normal control group, the proliferation activity of the HDF cells of the oxidation injury group is obviously reduced; compared with the oxidative damage group, the proliferation activity of the HDF cells in the polypeptide protection group is obviously improved. This indicates that the proliferation activity of HDF cells in oxidative damage environment is significantly reduced, whereas the resistance of HDF cells to oxidative damage is significantly improved and the ability to resist oxidative damage is significantly enhanced after predrying with the active polypeptide prepared in example 1.
TABLE 1 cell proliferation Activity of each group
2. Effect of active Polypeptides on Catalase Activity in human skin fibroblasts
Catalase activity in each group of cells as shown in table 2 and fig. 3, the catalase activity in the human skin fibroblasts of the polypeptide group was significantly increased compared to the control group, and was positively correlated to the administration concentration of the active polypeptide. As the skilled person knows, the activity of catalase is directly related to the antioxidant capacity of the cell, and the higher the activity of catalase is, the stronger the antioxidant capacity of the cell is.
TABLE 2 Catalase Activity in groups of cells
3. Effect of active Polypeptides on the content of type I collagen and elastin in human skin fibroblasts
The contents of type I collagen and elastin in each group of cells are shown in fig. 4, and compared with the control group, the contents of type I collagen and elastin in the human skin fibroblasts of the polypeptide group are obviously increased.
Important manifestations of skin aging include: the skin's antioxidant capacity decreases, leading to aging; the collagen and elastin are gradually lost, and wrinkles and sagging occur. The tests show that the active polypeptide provided by the invention can improve the antioxidant capacity of human skin fibroblasts on one hand, which is probably related to that the active polypeptide can improve the activity of catalase in the human skin fibroblasts; on the other hand, the content of type I collagen and elastin in human skin fibroblasts can be increased, so that skin wrinkles and sagging can be overcome. Therefore, the active polypeptide provided by the invention has the effect of resisting skin aging, and can be used as an anti-aging active component for preparing anti-aging cosmetics.
Claims (1)
1. Use of an active ingredient for the preparation of an anti-ageing cosmetic, the anti-ageing being an increase in the collagen and elastin content of the skin, the active ingredient being prepared by:
step S1, degreasing: pulverizing dried folium Artemisiae Argyi, defatting with petroleum ether under reflux, defatting with 10L petroleum ether for 2 times per kg folium Artemisiae Argyi, each time for 1.5 hr;
step S2, extracting: extracting defatted folium Artemisiae Argyi with pH 9.0 sodium hydroxide aqueous solution at room temperature, centrifuging the extractive solution at 4500r/min for 10 min; wherein, each kilogram of folium Artemisiae Argyi is leached at room temperature for 2 times, each time for 8h, with 8L volume of sodium hydroxide aqueous solution;
step S3, decoloring: adding activated carbon into the centrifuged supernatant, stirring and decoloring, centrifuging and collecting the supernatant; wherein the dosage of the active carbon is 0.2% w/v, the decoloring temperature is 75 ℃, and the decoloring time is 30 min;
step S4, alcohol precipitation: adding anhydrous ethanol into the supernatant after decolorization and centrifugation to make the final volume percentage concentration of ethanol be 60%, fully stirring, standing at 4 ℃ for 12h, centrifuging at the rotating speed of 8000r/min for 20min, collecting the supernatant, performing rotary evaporation at 50 ℃ to remove ethanol, and freeze-drying to obtain crude extract of folium Artemisiae Argyi;
step S5, gel column chromatography: dissolving crude extract of folium Artemisiae Argyi with distilled water to obtain 10mg/mL solution, loading onto Sephadex G-25 gel chromatographic column with inner diameter and bed height ratio of 1:10, loading onto 1/10 with bed volume, eluting with PBS buffer solution at flow rate of 1mL/min, monitoring with protein nucleic acid detector at wavelength of 280nm, collecting eluate corresponding to the 2 nd elution peak, and freeze drying.
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| CN113527422B (en) * | 2021-07-10 | 2024-02-09 | 浙江省农业科学院 | Polypeptide FLCQWP with antioxidant function and application thereof |
| CN114381488B (en) * | 2022-03-02 | 2024-08-09 | 上海瑞漾生物技术有限公司 | Active peptide, application of active peptide in resisting skin aging and commercialized application in cosmetics |
| CN114805485A (en) * | 2022-05-26 | 2022-07-29 | 青岛麦迪赛斯医疗技术有限公司 | Chlorella bioactive peptide and application thereof in preparation of anti-aging essence |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101791326A (en) * | 2009-09-09 | 2010-08-04 | 安徽海鑫中药饮片有限公司 | Method for extracting and purifying flavonoids of artemisia argyi |
| CN104861015A (en) * | 2015-01-16 | 2015-08-26 | 李玉山 | Synergistic clean extraction method of effective components in licorice root |
| CN105567774A (en) * | 2016-01-22 | 2016-05-11 | 杏辉天力（杭州）药业有限公司 | Walnut oligopeptide powder and preparation method and application thereof |
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| CN105878094A (en) * | 2014-12-05 | 2016-08-24 | 江南大学 | Preparation method of cosmetic containing tea seed antioxidant peptide |
| CN105079064A (en) * | 2015-07-30 | 2015-11-25 | 无限极（中国）有限公司 | Traditional Chinese medicine extract and preparation method and application thereof |
| CN107468585A (en) * | 2017-09-13 | 2017-12-15 | 泾县麦蓝网络技术服务有限公司 | Improve the obscure dark yellow composition of face and its preparation and the application in cosmetics |
| CN108660175B (en) * | 2018-07-08 | 2019-04-26 | 杭州嗣爵生物科技有限公司 | A kind of plant source polypeptide and the application in cosmetics |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101791326A (en) * | 2009-09-09 | 2010-08-04 | 安徽海鑫中药饮片有限公司 | Method for extracting and purifying flavonoids of artemisia argyi |
| CN104861015A (en) * | 2015-01-16 | 2015-08-26 | 李玉山 | Synergistic clean extraction method of effective components in licorice root |
| CN105567774A (en) * | 2016-01-22 | 2016-05-11 | 杏辉天力（杭州）药业有限公司 | Walnut oligopeptide powder and preparation method and application thereof |
Non-Patent Citations (3)
| Title |
|---|
| 艾叶研究进展概述;王惠君等;《江苏农业科学》;20151231;第43卷(第8期);15-19 * |
| 艾草抗氧化活性物质的提取分离;况伟等;《中国食品添加剂》;20151231(第6期);109-113 * |
| 艾蒿水提物对脂多糖刺激的肉仔鸡免疫和抗氧化功能的影响及其机理研究;张鹏飞;《中国博士学位论文全文数据库 农业科技辑》;中国学术期刊（光盘版）电子杂志社;20191115(第11期);D050-6 * |
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