CN111718979B - 基因扩增参考品及其应用 - Google Patents
基因扩增参考品及其应用 Download PDFInfo
- Publication number
- CN111718979B CN111718979B CN202010780111.4A CN202010780111A CN111718979B CN 111718979 B CN111718979 B CN 111718979B CN 202010780111 A CN202010780111 A CN 202010780111A CN 111718979 B CN111718979 B CN 111718979B
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- amplification
- dna
- target gene
- gene amplification
- gene
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- 230000004544 DNA amplification Effects 0.000 title claims abstract description 99
- 239000013558 reference substance Substances 0.000 title abstract description 16
- 230000003321 amplification Effects 0.000 claims abstract description 64
- 238000003199 nucleic acid amplification method Methods 0.000 claims abstract description 64
- 238000000034 method Methods 0.000 claims abstract description 50
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 50
- 238000007847 digital PCR Methods 0.000 claims description 12
- 238000012165 high-throughput sequencing Methods 0.000 claims description 6
- 238000012795 verification Methods 0.000 abstract description 14
- 238000002360 preparation method Methods 0.000 description 18
- 238000001514 detection method Methods 0.000 description 17
- 239000003153 chemical reaction reagent Substances 0.000 description 10
- 101150105382 MET gene Proteins 0.000 description 8
- 238000007796 conventional method Methods 0.000 description 7
- 238000005516 engineering process Methods 0.000 description 7
- 238000011156 evaluation Methods 0.000 description 7
- 238000012360 testing method Methods 0.000 description 7
- 238000004458 analytical method Methods 0.000 description 5
- 230000000694 effects Effects 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 238000012946 outsourcing Methods 0.000 description 3
- 102100031181 Glyceraldehyde-3-phosphate dehydrogenase Human genes 0.000 description 2
- 206010058467 Lung neoplasm malignant Diseases 0.000 description 2
- 238000012356 Product development Methods 0.000 description 2
- 238000010790 dilution Methods 0.000 description 2
- 239000012895 dilution Substances 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 238000012224 gene deletion Methods 0.000 description 2
- 108020004445 glyceraldehyde-3-phosphate dehydrogenase Proteins 0.000 description 2
- 201000005202 lung cancer Diseases 0.000 description 2
- 208000020816 lung neoplasm Diseases 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 206010006187 Breast cancer Diseases 0.000 description 1
- 208000026310 Breast neoplasm Diseases 0.000 description 1
- 108700039887 Essential Genes Proteins 0.000 description 1
- 108010014173 Factor X Proteins 0.000 description 1
- 101150054472 HER2 gene Proteins 0.000 description 1
- 239000002146 L01XE16 - Crizotinib Substances 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- KTEIFNKAUNYNJU-GFCCVEGCSA-N crizotinib Chemical compound O([C@H](C)C=1C(=C(F)C=CC=1Cl)Cl)C(C(=NC=1)N)=CC=1C(=C1)C=NN1C1CCNCC1 KTEIFNKAUNYNJU-GFCCVEGCSA-N 0.000 description 1
- 229960005061 crizotinib Drugs 0.000 description 1
- 238000012217 deletion Methods 0.000 description 1
- 230000037430 deletion Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- -1 e.g. Proteins 0.000 description 1
- 108700020302 erbB-2 Genes Proteins 0.000 description 1
- 238000012854 evaluation process Methods 0.000 description 1
- 239000007850 fluorescent dye Substances 0.000 description 1
- 238000007901 in situ hybridization Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 238000011275 oncology therapy Methods 0.000 description 1
- 238000003752 polymerase chain reaction Methods 0.000 description 1
- 238000011002 quantification Methods 0.000 description 1
- 238000012113 quantitative test Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 150000003384 small molecules Chemical class 0.000 description 1
- 229940126585 therapeutic drug Drugs 0.000 description 1
- 229960000575 trastuzumab Drugs 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
-
- G—PHYSICS
- G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
- G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
- G16B20/00—ICT specially adapted for functional genomics or proteomics, e.g. genotype-phenotype associations
- G16B20/10—Ploidy or copy number detection
-
- G—PHYSICS
- G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
- G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
- G16B30/00—ICT specially adapted for sequence analysis involving nucleotides or amino acids
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biophysics (AREA)
- Wood Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- Genetics & Genomics (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Immunology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Computational Biology (AREA)
- Evolutionary Biology (AREA)
- Medical Informatics (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Theoretical Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Description
样本 | DNA类型 | 初始扩增倍数 |
MET扩增阳性样本 | gDNA | 12.5 |
MET野生型样本 | gDNA | 1.1 |
MET扩增阳性样本 | cfDNA | 13.1 |
MET野生型样本 | cfDNA | 1.2 |
Claims (4)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202010724313 | 2020-07-24 | ||
CN2020107243137 | 2020-07-24 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN111718979A CN111718979A (zh) | 2020-09-29 |
CN111718979B true CN111718979B (zh) | 2021-03-23 |
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Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
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CN202010780111.4A Active CN111718979B (zh) | 2020-07-24 | 2020-08-05 | 基因扩增参考品及其应用 |
Country Status (1)
Country | Link |
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CN (1) | CN111718979B (zh) |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101821619A (zh) * | 2007-09-07 | 2010-09-01 | 弗卢丁公司 | 拷贝数变化确定、方法和*** |
CN106282165A (zh) * | 2016-08-24 | 2017-01-04 | 成都罗宁生物科技有限公司 | 一种植物内生菌16S rRNA基因扩增方法及应用 |
CN110878333A (zh) * | 2019-05-15 | 2020-03-13 | 嘉兴雅康博医学检验所有限公司 | 一种制备基因突变参考品的方法 |
CN110894524A (zh) * | 2019-05-13 | 2020-03-20 | 嘉兴雅康博医学检验所有限公司 | 一种快速制备基因突变参考品的方法 |
-
2020
- 2020-08-05 CN CN202010780111.4A patent/CN111718979B/zh active Active
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101821619A (zh) * | 2007-09-07 | 2010-09-01 | 弗卢丁公司 | 拷贝数变化确定、方法和*** |
CN106282165A (zh) * | 2016-08-24 | 2017-01-04 | 成都罗宁生物科技有限公司 | 一种植物内生菌16S rRNA基因扩增方法及应用 |
CN110894524A (zh) * | 2019-05-13 | 2020-03-20 | 嘉兴雅康博医学检验所有限公司 | 一种快速制备基因突变参考品的方法 |
CN110878333A (zh) * | 2019-05-15 | 2020-03-13 | 嘉兴雅康博医学检验所有限公司 | 一种制备基因突变参考品的方法 |
Non-Patent Citations (1)
Title |
---|
Certified DNA Reference Materials to Compare HER2 Gene Amplification Measurements Using Next-Generation Sequencing Methods;Lih, Chih-Jian; Si, Han; Das, Biswajit; 等.;《JOURNAL OF MOLECULAR DIAGNOSTICS》;20160930;第18卷(第5期);全文 * |
Also Published As
Publication number | Publication date |
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CN111718979A (zh) | 2020-09-29 |
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GR01 | Patent grant | ||
GR01 | Patent grant | ||
PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: Gene amplification reference material and its application Effective date of registration: 20210531 Granted publication date: 20210323 Pledgee: Agricultural Bank of China Limited by Share Ltd. South Lake branch Pledgor: JIAXING ACCB DIAGNOSTICS Ltd. Registration number: Y2021330000490 |
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PE01 | Entry into force of the registration of the contract for pledge of patent right | ||
PC01 | Cancellation of the registration of the contract for pledge of patent right |
Granted publication date: 20210323 Pledgee: Agricultural Bank of China Limited by Share Ltd. South Lake branch Pledgor: JIAXING ACCB DIAGNOSTICS Ltd. Registration number: Y2021330000490 |
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PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: Gene amplification reference materials and their applications Granted publication date: 20210323 Pledgee: Agricultural Bank of China Limited by Share Ltd. South Lake branch Pledgor: JIAXING ACCB DIAGNOSTICS Ltd. Registration number: Y2024330001048 |