CN111693353A - Detection system integrated with dyeing function and used for microscopic examination - Google Patents
Detection system integrated with dyeing function and used for microscopic examination Download PDFInfo
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- CN111693353A CN111693353A CN202010637572.6A CN202010637572A CN111693353A CN 111693353 A CN111693353 A CN 111693353A CN 202010637572 A CN202010637572 A CN 202010637572A CN 111693353 A CN111693353 A CN 111693353A
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- 238000004043 dyeing Methods 0.000 title claims abstract description 30
- 238000001514 detection method Methods 0.000 title claims abstract description 13
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 13
- 239000011521 glass Substances 0.000 claims abstract description 10
- 239000003480 eluent Substances 0.000 claims abstract description 7
- 238000007689 inspection Methods 0.000 claims abstract description 6
- 238000010186 staining Methods 0.000 claims description 29
- 230000003287 optical effect Effects 0.000 claims description 6
- 238000010828 elution Methods 0.000 claims description 5
- 239000012192 staining solution Substances 0.000 claims description 3
- 206010063385 Intellectualisation Diseases 0.000 claims 1
- 230000010354 integration Effects 0.000 abstract description 4
- 230000000694 effects Effects 0.000 abstract description 3
- 238000000034 method Methods 0.000 description 6
- 239000000243 solution Substances 0.000 description 4
- 230000009471 action Effects 0.000 description 3
- 239000007788 liquid Substances 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 235000013601 eggs Nutrition 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 230000005012 migration Effects 0.000 description 1
- 238000013508 migration Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000000877 morphologic effect Effects 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 239000012128 staining reagent Substances 0.000 description 1
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N1/31—Apparatus therefor
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/84—Systems specially adapted for particular applications
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/02—Objectives
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/06—Means for illuminating specimens
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/24—Base structure
- G02B21/248—Base structure objective (or ocular) turrets
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/34—Microscope slides, e.g. mounting specimens on microscope slides
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
- G01N2021/0106—General arrangement of respective parts
- G01N2021/0112—Apparatus in one mechanical, optical or electronic block
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- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Optics & Photonics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Multimedia (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Microscoopes, Condenser (AREA)
Abstract
The invention discloses a detection system integrated with a dyeing function for microscopic examination, which comprises a display rack, an object stage, an ocular, a camera set, an objective group, a dye solution reagent sample inlet pipe, a test sample inlet pipe, an eluent sample inlet pipe, a sample outlet pipe, an undyed counting chamber, a dyed counting chamber and a glass slide, wherein the object stage is arranged on the object stage; install objective group in the frame, install eyepiece and camera group directly over objective group, the objective group installs the objective table under, the top of objective table is provided with unstained counting chamber, dyed counting chamber and slide glass, dye liquor reagent sample inlet pipe, inspection sample inlet pipe, eluant sample inlet pipe, goes out the appearance pipe, dye liquor reagent sample inlet pipe, inspection sample inlet pipe, eluant sample inlet pipe install at the objective table left end, it installs at the objective table right-hand member to go out the appearance pipe. The invention realizes the integration and full automation of dyeing and microscopic examination in clinical examination work, improves the working accuracy and working efficiency, and ensures the dyeing quality and the examination effect.
Description
Technical Field
The invention relates to the technical field of microscope microscopic detection systems, in particular to a microscope microscopic detection system integrated with a dyeing function.
Background
In order to observe and study visible components such as bacteria, eggs, and cells in clinical tests clearly and accurately, the visible components are colored with a dye, and information such as the size and morphological structure of the colored components is observed with a microscope. Factors such as a dyeing method, dyeing time, a dyeing operation technology and the like can directly influence the quality of dyeing, so that the diagnosis accuracy in clinical examination is influenced. Most dyeing works are manual operation at present, and a lot of problems which cannot be avoided are generated in long-term manual dyeing works, for example, the dyeing work flow is not standardized, the dyeing quality is different, the dyeing work efficiency is not high, and the pollution and the injury to the body of an operator are caused by improper use of a dyeing solution. Dyeing instruments on the market at present also face the puzzles of not high accuracy, operation flexibility is low, degree of automation is not high etc..
In the routine clinical examination work of hospitals, the staining and the microscopic examination are separately operated, so that the method is not limited to the problems of the staining work, but also limited by the microscopic examination analysis system such as aging of the system, low shooting precision, low automation degree and the like, and the accuracy and the examination efficiency of the examination cannot be fundamentally improved.
Disclosure of Invention
Aiming at the defects of the prior art, the invention provides a detection system integrated with a microscope examination with a staining function, which comprises a microscope group and a staining group, wherein the microscope group comprises a rack, an objective table, an ocular lens, a camera group and an objective lens group; the staining group comprises a staining solution reagent sample inlet pipe, a test sample inlet pipe, an eluent sample inlet pipe, a sample outlet pipe, an unstained counting chamber, a staining counting chamber and a glass slide; install objective group in the frame, install eyepiece and camera group directly over objective group, the objective group installs the objective table under, the top of objective table is provided with unstained counting chamber, dyed counting chamber and slide glass, still be provided with dye liquor reagent sample inlet pipe, inspection sample inlet pipe, eluant sample inlet pipe, play appearance pipe on the objective table, dye liquor reagent sample inlet pipe, inspection sample inlet pipe, eluant sample inlet pipe install at the objective table left end, it installs at the objective table right-hand member to go out the appearance pipe.
Preferably, the objective lens group includes more than two objective lenses with different optical lengths, and the rotatable switching objective lens is mounted on the frame.
Preferably, the object stage can move horizontally on the frame to adjust the shooting position.
Preferably, the staining group sets different staining parameters or staining modes for the system to complete automatic staining, automatic elution and automatic sample discharge of the sample.
Preferably, the eyepiece is equipped with high pixel camera with the group of taking a picture in the camera group, can compatible different producers' high pixel camera, and with microscope group, dyeing group and can the automatic position regulation the objective table integration, makes the detecting system that the microscope mirror was examined possess the dyeing function to realize automatic and intelligent, promoted the accurate precision of mirror examination by a wide margin.
Compared with the prior art, the invention has the beneficial effects that:
1. the invention can convert the objective lenses with different optical lengths according to the physicochemical properties of a sample to be detected, adjust the light and the shooting angle, is provided with the latest high-pixel camera on the group surface of the camera, can be compatible with the cameras of different manufacturers, is matched with a microscopic examination system on the configuration of the objective table, fully automatically adjusts the shooting position, and greatly improves the detection accuracy.
2. The invention solves the problems of non-standardization of dyeing work and larger result in-out in actual clinical work, realizes the integration and full automation of dyeing and microscopic examination in clinical examination work, improves the working accuracy and working efficiency, and ensures the dyeing quality and the examination effect.
3. The microscope group and the staining group are integrated, microscopic examination can be performed after full-automatic staining, and the problem that the working accuracy is low due to the fact that the staining group and the microscope group cannot be integrated in the current market is solved.
Drawings
FIG. 1 is a schematic structural diagram of the present invention.
In the figure:
1-a staining reagent sampling tube; 2-inspecting the sample inlet tube; 3-eluent sample tube; 4-ocular and camera set; 5-an objective lens group; 6-unstained counting chamber; 7-staining counting chamber; 8-a sample outlet pipe; 9-an object stage; 10-a frame.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
As shown in fig. 1, a detection system integrated with a microscope examination of a staining function comprises a microscope set and a staining set, wherein the microscope set comprises a frame 10, an objective table 9, an ocular lens, a camera set 4 and an objective lens set 5, the objective lens set 5 comprises more than two objective lenses with different optical lengths, and the camera sets in the ocular lens and the camera set 4 are provided with high-pixel cameras and can be compatible with the high-pixel cameras of different manufacturers; the staining group comprises a staining solution reagent sample inlet pipe 1, a test sample inlet pipe 2, an eluent sample inlet pipe 3, a sample outlet pipe 8, an unstained counting chamber 6, a staining counting chamber 7 and a glass slide; install objective group 5 in the frame 10, the rotatable switching of objective on the objective group 5, eyepiece and camera set 4 are installed directly over the objective group 5, objective group 5 installs objective table 9 under, objective table 9 can adjust the shooting position in frame 10 upper horizontal migration, objective table 9's top is provided with unstained counting chamber 6, dyed counting chamber 7 and slide glass, still be provided with dye liquor reagent sample inlet pipe 1 on the objective table 9, test sample inlet pipe 2, eluant sample inlet pipe 3, play appearance pipe 8, dye liquor reagent sample inlet pipe 1, test sample inlet pipe 2, eluant sample inlet pipe 3 install at objective table 9 left end, it installs at objective table 9 right-hand member to go out appearance pipe 8.
Specifically, under the dyeing and elution settings of the system, samples are subjected to dyeing and elution in different reagents and different steps, firstly, a sample inlet pipe 2 is used for conveying a test sample to an undyed counting chamber 6 and a dyed counting chamber 7 respectively, then, a dye solution inlet pipe 1 is used for conveying dye solution for dyeing, then, an eluent inlet pipe 3 is used for conveying eluent for elution, after dyeing, an ocular lens and a camera group 4 are used for photographing, identifying and diagnosing, and after identifying and diagnosing, all liquid is output through a sample outlet pipe 8.
Specifically, the lens of the camera in the eyepiece and camera group 4 and the objective lenses with different optical lengths in the objective lens group 5 are switched and combined, and are focused on the mixed liquid of the glass slide on the objective table 9, the unstained counting chamber 6 and the stained counting chamber 7, and the objective lenses with different optical lengths are switched to shoot the target objects in the glass slide, the unstained counting chamber 6 and the stained counting chamber 6, so that the integration and full automation of staining and microscopic examination in the clinical examination work are realized, the working accuracy and working efficiency are improved, and the staining quality and the examination effect are ensured.
It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.
Claims (5)
1. A detection system integrated with a staining function for microscopic examination is characterized by comprising a microscope set and a staining set, wherein the microscope set comprises a rack, an objective table, an ocular lens, a camera set and an objective lens set; the staining group comprises a staining solution reagent sample inlet pipe, a test sample inlet pipe, an eluent sample inlet pipe, a sample outlet pipe, an unstained counting chamber, a staining counting chamber and a glass slide; install objective group in the frame, install eyepiece and camera group directly over objective group, the objective group installs the objective table under, the top of objective table is provided with unstained counting chamber, dyed counting chamber and slide glass, still be provided with dye liquor reagent sample inlet pipe, inspection sample inlet pipe, eluant sample inlet pipe, play appearance pipe on the objective table, dye liquor reagent sample inlet pipe, inspection sample inlet pipe, eluant sample inlet pipe install at the objective table left end, it installs at the objective table right-hand member to go out the appearance pipe.
2. The system of claim 1, wherein the objective lens group comprises two or more objective lenses with different optical lengths, and the rotatable switching objective lens is mounted on the frame.
3. The microscope microscopic examination detection system integrated with dyeing function as claimed in claim 1, wherein said stage can be moved horizontally on the frame to adjust the photographing position.
4. The microscope microscopic detection system integrated with the staining function as claimed in claim 1, wherein the staining group performs automatic staining, automatic elution and automatic sample output on the sample by setting different staining parameters or staining modes for the system.
5. The microscope microscopic examination detection system integrated with the dyeing function as claimed in claim 1, wherein the eyepiece and the camera set are equipped with high-pixel cameras, and are compatible with high-pixel cameras of different manufacturers, and the microscope set, the dyeing set and the objective table capable of automatically adjusting the position are integrated, so that the microscope microscopic examination detection system has the dyeing function, automation and intellectualization are realized, and the accuracy of microscopic examination is greatly improved.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010637572.6A CN111693353A (en) | 2020-07-05 | 2020-07-05 | Detection system integrated with dyeing function and used for microscopic examination |
| CN202110715265.XA CN113447337A (en) | 2020-07-05 | 2021-06-26 | Detection system integrated with dyeing function and used for microscopic examination |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010637572.6A CN111693353A (en) | 2020-07-05 | 2020-07-05 | Detection system integrated with dyeing function and used for microscopic examination |
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| CN111693353A true CN111693353A (en) | 2020-09-22 |
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| CN202010637572.6A Pending CN111693353A (en) | 2020-07-05 | 2020-07-05 | Detection system integrated with dyeing function and used for microscopic examination |
| CN202110715265.XA Pending CN113447337A (en) | 2020-07-05 | 2021-06-26 | Detection system integrated with dyeing function and used for microscopic examination |
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| CN202110715265.XA Pending CN113447337A (en) | 2020-07-05 | 2021-06-26 | Detection system integrated with dyeing function and used for microscopic examination |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113433126A (en) * | 2021-06-24 | 2021-09-24 | 褚崇胜 | Rice pollen fertility microscopic examination instrument |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN116297444B (en) * | 2023-01-31 | 2023-09-15 | 中国人民解放军总医院第七医学中心 | Device for assisting three-dimensional image equipment in chromosome abnormality detection |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| KR100584678B1 (en) * | 2005-11-02 | 2006-05-29 | 브이엔에프 이노베이션 주식회사 | Optical mesurement apparatus |
| CN1811425A (en) * | 2006-01-28 | 2006-08-02 | 重庆大学 | Silicon microchannel array erythrocyte rheological analyzing method and apparatus |
| CN101762585A (en) * | 2009-05-05 | 2010-06-30 | 孔兵 | Cell morphology microscopic examination device and method based on staining technology |
| CN103695305B (en) * | 2013-12-18 | 2016-06-22 | 麦克奥迪实业集团有限公司 | A kind of gray scale photographic head for micrometron and cell comprehensive analysis device |
| CN106019557A (en) * | 2016-08-17 | 2016-10-12 | 珠海科域生物工程有限公司 | Multi-layer photographing method and photographing system for microscopic examination |
| CN207621255U (en) * | 2017-12-20 | 2018-07-17 | 河南华韵物联科技有限公司 | The shockproof footing of garment devices |
| CN108231135A (en) * | 2018-03-05 | 2018-06-29 | 四川忠辉电子科技有限公司 | A kind of variable rigidity formula instrument and meter vibration abatement |
| CN208860653U (en) * | 2018-09-13 | 2019-05-14 | 郑州安图生物工程股份有限公司 | Sediments microscope inspection slide |
| CN209656981U (en) * | 2019-05-23 | 2019-11-19 | 楚雄医药高等专科学校 | A kind of novel medical test microscope |
| CN110780440B (en) * | 2019-11-12 | 2021-08-24 | 四川沃文特生物技术有限公司 | Photographic microscope and method for rapidly photographing by using same |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113433126A (en) * | 2021-06-24 | 2021-09-24 | 褚崇胜 | Rice pollen fertility microscopic examination instrument |
| CN113433126B (en) * | 2021-06-24 | 2024-03-08 | 褚崇胜 | Rice pollen fertility microscopic examination instrument |
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| CN113447337A (en) | 2021-09-28 |
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