CN111661833A - Nano carbon dots (particles) extracted from plant ash and application thereof - Google Patents
Nano carbon dots (particles) extracted from plant ash and application thereof Download PDFInfo
- Publication number
- CN111661833A CN111661833A CN201910167161.2A CN201910167161A CN111661833A CN 111661833 A CN111661833 A CN 111661833A CN 201910167161 A CN201910167161 A CN 201910167161A CN 111661833 A CN111661833 A CN 111661833A
- Authority
- CN
- China
- Prior art keywords
- plant ash
- carbon dots
- particles
- application
- dots
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- VNWKTOKETHGBQD-UHFFFAOYSA-N methane Chemical compound C VNWKTOKETHGBQD-UHFFFAOYSA-N 0.000 title claims abstract description 18
- 239000002245 particle Substances 0.000 title claims abstract description 12
- 229910021392 nanocarbon Inorganic materials 0.000 title abstract description 5
- 229910052799 carbon Inorganic materials 0.000 claims abstract description 6
- 238000000502 dialysis Methods 0.000 claims abstract description 5
- 238000000034 method Methods 0.000 claims abstract description 3
- 230000005540 biological transmission Effects 0.000 claims abstract 2
- 201000010099 disease Diseases 0.000 claims abstract 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims abstract 2
- 230000008635 plant growth Effects 0.000 claims description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 4
- 238000000108 ultra-filtration Methods 0.000 claims description 2
- 241000607479 Yersinia pestis Species 0.000 claims 1
- 239000000706 filtrate Substances 0.000 claims 1
- 230000000717 retained effect Effects 0.000 claims 1
- 230000007226 seed germination Effects 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 3
- 230000012010 growth Effects 0.000 abstract description 3
- 230000001737 promoting effect Effects 0.000 abstract description 3
- 241000238631 Hexapoda Species 0.000 abstract 1
- 238000002347 injection Methods 0.000 abstract 1
- 239000007924 injection Substances 0.000 abstract 1
- 230000002262 irrigation Effects 0.000 abstract 1
- 238000003973 irrigation Methods 0.000 abstract 1
- 239000002904 solvent Substances 0.000 abstract 1
- 241000196324 Embryophyta Species 0.000 description 17
- 239000002956 ash Substances 0.000 description 17
- 229920000936 Agarose Polymers 0.000 description 5
- 239000000243 solution Substances 0.000 description 5
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 4
- 239000011852 carbon nanoparticle Substances 0.000 description 4
- 244000046052 Phaseolus vulgaris Species 0.000 description 3
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 3
- 240000004922 Vigna radiata Species 0.000 description 3
- 235000010721 Vigna radiata var radiata Nutrition 0.000 description 3
- 235000011469 Vigna radiata var sublobata Nutrition 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- 239000010902 straw Substances 0.000 description 3
- 239000010803 wood ash Substances 0.000 description 3
- 230000004071 biological effect Effects 0.000 description 2
- 230000005284 excitation Effects 0.000 description 2
- 238000002073 fluorescence micrograph Methods 0.000 description 2
- 238000010587 phase diagram Methods 0.000 description 2
- OSBLTNPMIGYQGY-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;2-[2-[bis(carboxymethyl)amino]ethyl-(carboxymethyl)amino]acetic acid;boric acid Chemical compound OB(O)O.OCC(N)(CO)CO.OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O OSBLTNPMIGYQGY-UHFFFAOYSA-N 0.000 description 1
- 235000002918 Fraxinus excelsior Nutrition 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 241000209504 Poaceae Species 0.000 description 1
- 239000008051 TBE buffer Substances 0.000 description 1
- 241000209140 Triticum Species 0.000 description 1
- 235000021307 Triticum Nutrition 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 235000005822 corn Nutrition 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 239000000385 dialysis solution Substances 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 230000035784 germination Effects 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- XAEFZNCEHLXOMS-UHFFFAOYSA-M potassium benzoate Chemical compound [K+].[O-]C(=O)C1=CC=CC=C1 XAEFZNCEHLXOMS-UHFFFAOYSA-M 0.000 description 1
- 239000003755 preservative agent Substances 0.000 description 1
- 230000002335 preservative effect Effects 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C01—INORGANIC CHEMISTRY
- C01B—NON-METALLIC ELEMENTS; COMPOUNDS THEREOF; METALLOIDS OR COMPOUNDS THEREOF NOT COVERED BY SUBCLASS C01C
- C01B32/00—Carbon; Compounds thereof
- C01B32/15—Nano-sized carbon materials
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y30/00—Nanotechnology for materials or surface science, e.g. nanocomposites
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y40/00—Manufacture or treatment of nanostructures
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D9/00—Other inorganic fertilisers
Landscapes
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Nanotechnology (AREA)
- Organic Chemistry (AREA)
- Inorganic Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Materials Engineering (AREA)
- Crystallography & Structural Chemistry (AREA)
- Condensed Matter Physics & Semiconductors (AREA)
- Physics & Mathematics (AREA)
- Composite Materials (AREA)
- Manufacturing & Machinery (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Fertilizers (AREA)
Abstract
The invention relates to a method for extracting, separating and purifying nano carbon dots (particles) from plant ash, which comprises the following steps: extracting plant ash with solvent, dialyzing with dialysis bag with molecular weight greater than 1000Da, or ultrafiltering to obtain carbon dots of plant ash, which exhibit blue fluorescence under ultraviolet lamp 365nm and have particle diameter less than 100nm measured by transmission electron microscope, and mainly comprise C, H, O and N; the invention also relates to application of the carbon dots in the agricultural field, and the carbon dots have the effects of promoting growth, sprouting, resisting diseases and insect hazards and the like after being applied to plants through injection, irrigation and other ways.
Description
Technical Field
The invention belongs to the field of agriculture, and particularly relates to preparation of plant ash carbon dots and application of the plant ash carbon dots in the field of agriculture.
Background
Plant ash has a long history of use as an agricultural fertilizer, and the substance acting on it is generally considered to be a potassium salt. The preparation method of the plant ash comprises the following steps:
ashes produced by burning herbaceous plants such as wheat straws, corn straws, rice straws, grasses and the like.
Disclosure of Invention
The invention extracts and separates nano carbon dots (particles) from plant ash for the first time, and discovers that the carbon dots of the plant ash have the biological effect of promoting the growth of plants for the first time.
The technical scheme is as follows: the invention provides a method for extracting and separating nano carbon dots (particles) from plant ash. The invention also provides the biological effect that the plant ash carbon dots have the effect of promoting the growth of plants.
The preparation method specifically comprises the following steps: extracting carbon dots (particles) from plant ash by decoction, and further separating and purifying by dialysis, ultrafiltration, chromatography, etc. to obtain high purity carbon dots.
Drawings
FIG. 1 is a graph of topographical features of carbon dots of plant ash
FIG. 1A is a phase diagram of a low resolution electron microscope morphology of grass-wood ash carbon nanoparticles
FIG. 1B is a high resolution electron microscope morphology phase diagram of grass-wood ash carbon nanoparticles
FIG. 1C is a fluorescence image of grass-wood ash carbon nanoparticle sets under ultraviolet excitation
FIG. 1D is a fluorescence image of control group under ultraviolet excitation
FIG. 2 is a graph showing the effect of plant ash carbon nanoparticles on bean sprout length
Detailed Description
Example 1:
decocting with pure water for 3 times, mixing the water solutions, concentrating, and dialyzing with 1KD dialysis bag to obtain high-purity plant ash solution.
Example 2 action of the carbon points of the plant Ash to promote the growth of Bean sprouts
Weighing 0.3g of agarose, placing the agarose in a triangular flask, adding 30mL of TBE buffer solution, placing the triangular flask in a microwave oven to heat the agarose until the agarose is dissolved, and adding different groups of components after the agarose is cooled to below 60 ℃.
Divided into two groups according to design: a the plant ash dialysis solution group, adding 5mL of the solution after the plant ash is boiled in water and dialyzed (removing small molecular compounds, only nano-scale carbon particles are left)
b control group: an equal volume of aqueous solution was added. Pouring the prepared gel into a triangular flask, standing at room temperature and cooling. After the gel is solidified, selecting fresh mung bean seeds, uniformly and fully placing the mung bean seeds on the gel in groups at random, sealing a triangular bottle with a preservative film and pricking air holes, placing the triangular bottle in a constant-temperature constant-humidity box, culturing at 25 ℃, and setting 12-hour light/12-hour dark to simulate day and night change.
Through measurement and statistics, the germination rates of mung beans between two groups are not different, while the carbon particle solution group is obviously improved compared with the control group in the growth length of bean sprouts, and the carbon particles are shown to be beneficial to the growth of plants.
Claims (2)
1. A method for extracting nanometer carbon dots (particles) from plant ash is provided.
Decocting plant ash with water, dialyzing with dialysis bag with molecular weight of 1-10 KDa, and collecting the fraction retained in the dialysis bag, or ultrafiltering with ultrafiltration tube of 10KD to obtain filtrate. The carbon quantum dots are characterized in that: blue fluorescence under an ultraviolet lamp of 365nm, and a particle size of 2-8nm measured by a transmission electron microscope, mainly comprising C, N and O element.
2. The application of the plant ash carbon dots in the agricultural field.
Including but not limited to, plant growth promotion, seed germination, and disease and pest resistance.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910167161.2A CN111661833A (en) | 2019-03-06 | 2019-03-06 | Nano carbon dots (particles) extracted from plant ash and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910167161.2A CN111661833A (en) | 2019-03-06 | 2019-03-06 | Nano carbon dots (particles) extracted from plant ash and application thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN111661833A true CN111661833A (en) | 2020-09-15 |
Family
ID=72381825
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910167161.2A Pending CN111661833A (en) | 2019-03-06 | 2019-03-06 | Nano carbon dots (particles) extracted from plant ash and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN111661833A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113115605A (en) * | 2021-05-20 | 2021-07-16 | 江南大学 | Method for promoting spinach seed germination by using carbon dots |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106590640A (en) * | 2016-10-27 | 2017-04-26 | 华南农业大学 | Application of carbon dots in light conversion of agricultural production as agricultural light conversion material |
-
2019
- 2019-03-06 CN CN201910167161.2A patent/CN111661833A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106590640A (en) * | 2016-10-27 | 2017-04-26 | 华南农业大学 | Application of carbon dots in light conversion of agricultural production as agricultural light conversion material |
Non-Patent Citations (1)
| Title |
|---|
| MEILING ZHANG ET AL.: ""Novel carbon dots derived from Schizonepetae Herba Carbonisata and investigation of their haemostatic efficacy"", 《ARTIFICIAL CELLS,NANOMEDICINE, AND BIOTECHNOLOGY》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113115605A (en) * | 2021-05-20 | 2021-07-16 | 江南大学 | Method for promoting spinach seed germination by using carbon dots |
| CN113115605B (en) * | 2021-05-20 | 2022-05-24 | 江南大学 | Method for promoting spinach seed germination by using carbon dots |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN112979353A (en) | Preparation method and application of low-cost environment-friendly carbon quantum dot nano fertilizer | |
| CN104719162B (en) | Method for screening dandelion salt-tolerance mutants | |
| CN106718934B (en) | A kind of Rhizoma Atractylodis Macrocephalae regenerating system directly breaking up adventitious bud using plumular axis and radicle | |
| CN104381321A (en) | Plant source biological composition and preparation method thereof as well as application of plant source biological composition to prevention and treatment of green onion maggots | |
| CN107409662A (en) | A kind of alternating method for reducing the tobacco bacterial wilt incidence of disease | |
| CN111661833A (en) | Nano carbon dots (particles) extracted from plant ash and application thereof | |
| CN101538304A (en) | Fungi mixture and method for preparing same | |
| Janarthanam et al. | Plant regeneration from leaf derived callus of Stevia rebaudiana Bertoni | |
| CN104041490A (en) | Granular holotrichia parallela slow-releasing sex attractant and preparation method thereof | |
| CN107125268A (en) | A kind of indoor potted landscape soil cultivation additive and preparation method | |
| CN110373196A (en) | A kind of tobacco-growing soil nanometer cadmium passivator and the preparation method and application thereof | |
| KR102226151B1 (en) | Method for producing coumestrol using leguminous plants | |
| Khalid et al. | Banana bunchy top disease in Pakistan | |
| CN114854402A (en) | Preparation method of fluorescent nano material capable of promoting growth of cherokee rose group culture seedling | |
| Cho et al. | A Study on soil suitability criteria for Liriopis platyphylla | |
| CN107494229B (en) | Method for regulating rooting of watermifoil by using allelopathic effect of yellow flag | |
| CN1259822C (en) | Snow lotus flavone active ingredient production method by cultivating acaleph snow lotus trichome | |
| Ojeniyi | Investigation of ploughing requirement for the establishment of cowpea (Vignea unguiculata) | |
| CN101289651A (en) | Sinkiang alkanna tinctoria cell lines and process for producing sinkiang alkanna tinctoria alkannin by cell culture | |
| CN107011081B (en) | Slow-release pesticide-fertilizer composition | |
| EP0479459B1 (en) | Production of quercetin glucuronide and cultured cells containing the same | |
| RU2611520C1 (en) | Method of producing silver nanoparticles | |
| KR102107457B1 (en) | Composition for repelling aphid comprising Ginseng extracts | |
| CN103493844A (en) | Method for preparing neem cell culture insecticide | |
| CN108912235B (en) | Preparation method of agrimony polysaccharide and plant salt-resistant regulator |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20200915 |
|
| RJ01 | Rejection of invention patent application after publication |