CN111646640A - Artificial aquatic weed and application thereof - Google Patents

Artificial aquatic weed and application thereof Download PDF

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Publication number
CN111646640A
CN111646640A CN202010452659.6A CN202010452659A CN111646640A CN 111646640 A CN111646640 A CN 111646640A CN 202010452659 A CN202010452659 A CN 202010452659A CN 111646640 A CN111646640 A CN 111646640A
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slow
particles
artificial
release
microbial inoculum
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张文龙
史蒙
李轶
陆宇苗
杨雪梅
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Hohai University HHU
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Hohai University HHU
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    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F9/00Multistage treatment of water, waste water or sewage
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F1/00Treatment of water, waste water, or sewage
    • C02F1/001Processes for the treatment of water whereby the filtration technique is of importance
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F1/00Treatment of water, waste water, or sewage
    • C02F1/28Treatment of water, waste water, or sewage by sorption
    • C02F1/281Treatment of water, waste water, or sewage by sorption using inorganic sorbents
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F1/00Treatment of water, waste water, or sewage
    • C02F1/28Treatment of water, waste water, or sewage by sorption
    • C02F1/283Treatment of water, waste water, or sewage by sorption using coal, charred products, or inorganic mixtures containing them
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2101/00Nature of the contaminant
    • C02F2101/10Inorganic compounds
    • C02F2101/105Phosphorus compounds
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2101/00Nature of the contaminant
    • C02F2101/10Inorganic compounds
    • C02F2101/16Nitrogen compounds, e.g. ammonia
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2101/00Nature of the contaminant
    • C02F2101/30Organic compounds
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2305/00Use of specific compounds during water treatment
    • C02F2305/06Nutrients for stimulating the growth of microorganisms
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00Biological treatment of water, waste water, or sewage
    • C02F3/02Aerobic processes
    • C02F3/08Aerobic processes using moving contact bodies
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00Biological treatment of water, waste water, or sewage
    • C02F3/34Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A20/00Water conservation; Efficient water supply; Efficient water use
    • Y02A20/40Protecting water resources
    • Y02A20/402River restoration

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  • Life Sciences & Earth Sciences (AREA)
  • Hydrology & Water Resources (AREA)
  • Engineering & Computer Science (AREA)
  • Environmental & Geological Engineering (AREA)
  • Water Supply & Treatment (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The invention discloses an artificial float grass and application thereof, wherein the artificial float grass comprises a middle rope, a floater, float grass fibers, a functional floating block, a functional substrate and a heavy weight which are connected in series by the middle rope; the functionalized floating block comprises slow-release microbial inoculum particles and a floating block carrier for bearing the slow-release microbial inoculum particles; the slow-release microbial inoculum particles adopt a composite microbial inoculum and are filled with signal molecules; the functionalized substrate comprises slow-release oxygen particles and a substrate carrier for carrying the slow-release oxygen particles. The artificial aquatic weed can continuously and slowly release microbial flora, signal molecules and oxygen, provides a place suitable for growth of microorganisms, and can quickly and effectively remove pollutants in a river water body.

Description

Artificial aquatic weed and application thereof
Technical Field
The invention belongs to the technical field of water ecological restoration, and particularly relates to artificial aquatic weeds and application thereof.
Background
In the process of continuously promoting urbanization construction in China, concrete channelization transformation is carried out on numerous urban rivers in consideration of aspects of river flood, shipping and the like. This results in many urban rivers being cut and bent to straighten, and bank protection and bottom are hardened, further resulting in the reduction of direct material exchange between rivers and soil, making it difficult for animals and plants such as natural aquatic weeds, fishes and the like to grow in rivers, and especially weakening the biodegradation of microorganisms on pollutants in rivers. Finally, the ecological function of the river becomes fragile, the self-purification capacity is reduced, and meanwhile, because a large amount of domestic sewage and industrial wastewater are discharged into the urban river channels, the river channels are always eutrophicated and even black and odorous.
Aiming at the problems of hardening, difficult natural aquatic weed growth, lack of places suitable for microorganism attachment growth, reduced self-cleaning capacity, eutrophication, black and odorous appearance and the like of the urban river, common restoration technologies comprise aeration, artificial aquatic weeds, ecological floating beds, artificial wetlands and the like. The artificial aquatic weed belongs to an in-situ ecological restoration technology, has the advantages of low investment, small engineering amount, small secondary pollution and the like compared with ex-situ restoration and physical and chemical restoration, and is gradually practically applied to urban rivers in recent years.
The artificial aquatic plant technology is essentially a biomembrane technology, and usually adopts an aquatic plant-imitating material with acid and alkali resistance, stain resistance and good mechanical property to imitate the purification principle of aquatic plants and biological fillers. It is not limited by transparency, illumination and the like, and is helpful for the attachment growth of microorganisms to form a stable biofilm. The common characteristics of the waterweeds currently used most include an Ackman ecological base, a string-shaped artificial waterweed, a clodinella type artificial waterweed, a biological zone type artificial waterweed and the like, and the waterweeds have the advantage of providing proper growth conditions for various microorganisms, so that the microorganisms can be attached to the surface of the waterweeds to adsorb and degrade pollutants.
However, the practical application of the artificial aquatic plants at present has some problems, such as: the biofilm on the aquatic weeds has long forming time, is unstable and is easy to be impacted by water power; the growth of functional microorganisms is limited by dissolved oxygen and nutrition; short repair duration, etc. In addition, many seriously polluted rivers lack a certain amount of functional microbial groups, and even ordinary artificial aquatic plants are placed in the rivers, the rivers cannot have good repairing effects.
Disclosure of Invention
The invention aims to overcome the defects in the prior art, and provides an artificial aquatic plant and application thereof, which can continuously and slowly release microbial flora, signal molecules and oxygen, provide a suitable place for microbial growth, and strengthen the self-cleaning capacity of rivers.
In order to solve the technical problems, the invention adopts the following technical scheme:
on one hand, the invention provides an artificial aquatic plant, which comprises a middle rope, a floater, aquatic plant fibers, a functional floating block, a functional substrate and a heavy weight, wherein the floater, the aquatic plant fibers, the functional floating block, the functional substrate and the heavy weight are connected in series by the middle rope; the functionalized floating block comprises slow-release microbial inoculum particles and a floating block carrier for bearing the slow-release microbial inoculum particles; the slow-release microbial inoculum particles adopt a composite microbial inoculum and are filled with signal molecules; the functionalized substrate comprises slow-release oxygen particles and a substrate carrier for carrying the slow-release oxygen particles. Furthermore, the functionalized floating blocks are multiple, and the interval between every two adjacent functionalized floating blocks is 0.5-1 m.
Further, the compound microbial inoculum comprises bacillus subtilis, nitrobacteria, denitrifying bacteria and photosynthetic bacteria.
Further, the floating block carrier is an open-cell polyurethane foam carrier.
Further, the signal molecule includes acylated homoserine lactone and AI-2.
Further, the sustained-release microbial inoculum particles take sodium alginate and polyvinyl alcohol as composite embedding materials, and the dosage ratio of the sodium alginate to the polyvinyl alcohol is 1-3: 1; with boric acid and CaCl2The mixed solution of (2) is a cross-linking agent.
Further, the substrate carrier is a composite carrier of biochar and polyurethane.
Furthermore, the slow-release oxygen particles take calcium peroxide as an oxygen release agent, polyhydroxy fatty acid ester as an embedding agent, and the mass ratio of the polyhydroxy fatty acid ester to the calcium peroxide is 1-3: 1.
Furthermore, the aquatic weed fiber is made of polypropylene and vinylon and is uniformly fixed on the middle rope in a mode of annular filament group.
On the other hand, the invention also provides application of the artificial aquatic weeds in river ecological restoration.
Compared with the prior art, the invention has the following beneficial effects:
1. the artificial aquatic plant adopts the bionic aquatic plant fiber, combines the functional floating block and the substrate, can strengthen the processes of natural film formation, nitrogen circulation and the like of a water body on the basis of repairing the polluted river channel by utilizing the functional microorganisms, and promotes the microorganisms to form a biological film with pollutant degradation capability on the grass fiber, thereby stabilizing the water ecological environment of the river channel.
2. The functional floating block adopted by the invention can slowly release functional microorganisms through the slow release particles, wherein the released nitrobacteria, denitrifying bacteria and bacillus subtilis have the effects of promoting denitrification and degrading organic matters, and meanwhile, a biomembrane is formed on the waterweed fibers, so that the microbial community structure of a river water body can be improved, signal molecules such as acylated homoserine lactone and the like are released, the microorganisms are promoted to quickly form the biomembrane, and the impact resistance of the biomembrane is enhanced.
3. The invention adopts the functionalized substrate as the biochar and polyurethane composite carrier, can provide a microenvironment suitable for growth of microorganisms, and uses the biochar as a slow release carbon source and an electron transmission medium, can adsorb pollutants such as nitrogen, phosphorus and the like, can slowly release oxygen required by the microorganisms through the slow release particles, is beneficial to the growth and film formation of the microorganisms, simultaneously improves the anoxic environment of bottom mud, and improves the occurrence forms of elements such as phosphorus, iron and the like.
4. The invention synthesizes three functions of physics, chemistry and biology to repair the urban river and enhance the self-cleaning capability of the urban river, particularly comprises filtration, adsorption, micro-aeration, biodegradation and the like, has the advantages of obvious repair effect, quick response, simple structure, low investment and operation cost and the like, can obtain better water body treatment effect, is simple and convenient to set in practical application, runs stably, and can be widely applied to ecological repair of the river.
Drawings
Fig. 1 is a schematic structural view of an artificial aquatic plant according to a first embodiment of the present invention;
FIG. 2 is an SEM image of a three-dimensional spongy biochar provided by the embodiment of the invention;
fig. 3 is a DO variation curve of the sampling section of the experimental black and odorous river provided in the second embodiment of the present invention;
FIG. 4 is a diagram of NH of sampling cross section of experimental black and odorous river according to the second embodiment of the present invention3-N variation curve;
fig. 5 is a TP variation curve of the sampling cross-section of the experimental black and odorous river provided in the second embodiment of the present invention;
fig. 6 is a LefSe evolutionary branch diagram of an experimental black and odorous river sampling section according to the second embodiment of the present invention.
In the figure: 1. a float; 2. aquatic weed fibers; 3. a functionalized floating block; 4. a functionalized substrate; 5. heavy bob; 6. a middle rope.
Detailed Description
Embodiments of the present invention will be described in detail below with reference to the accompanying drawings, which are provided for enabling those of ordinary skill in the art to clearly understand the present invention and to implement the present invention based on the description herein. The drawings and detailed description are not intended to limit the invention, which is defined by the appended claims.
The first embodiment is as follows:
as shown in fig. 1, the artificial float grass provided by the embodiment of the invention comprises a middle rope 6, a floater 1 is arranged at the uppermost end of the middle rope 6, a functional floating block 3, a functional substrate 4 and a heavy weight 5 are sequentially connected in series at the lower end of the floater 1, and the float grass fibers 2 are uniformly distributed on the middle rope 6.
The microorganisms can form a biofilm on the waterweed fibers 2, thereby improving the microbial community structure of the river water body. The float grass fiber 2 can be polypropylene or vinylon, and the material of the float grass fiber 2 can be replaced by other materials, such as nylon, terylene, etc. The aquatic weed fiber 2 can be uniformly fixed on the middle rope 6 in a mode of annular filament group, and the middle rope 6 can be made of mixed polypropylene fiber.
In particular, the functionalized float 3Comprises slow-release microbial inoculum particles and a floating block carrier, wherein the floating block carrier is used for carrying the slow-release microbial inoculum. In the embodiment of the invention, the sustained-release microbial inoculum particles take sodium alginate and polyvinyl alcohol as composite embedding materials, the dosage ratio of the sodium alginate to the polyvinyl alcohol is 1-3:1, and boric acid and CaCl are used2The mixed solution is a cross-linking agent, the cross-linking time is 24 hours, and the cross-linking agent is formed by an injector after being mechanically stirred and uniformly mixed. The slow-release microbial inoculum particles adopt a compound microbial inoculum and are filled with signal molecules. Specifically, the composite microbial agent comprises bacillus subtilis, nitrobacteria, denitrifying bacteria and photosynthetic bacteria, the mass ratio can be 1-3:1-4:1-2:1-2, and the filled signal molecules comprise acylated homoserine lactone and AI-2.
A preparation method of the sustained-release microbial inoculum particles comprises the following steps:
s1, respectively weighing 7g of sodium alginate and 3g of polyvinyl alcohol, and adding 240mL of distilled water, wherein the total mass concentration of the sodium alginate and the polyvinyl alcohol is 4%. Heating in water bath to dissolve completely, stirring, and performing 20min for 1.15kg/cm2Sterilizing under high pressure, and cooling;
s2, respectively culturing the bacillus subtilis, the nitrobacteria, the denitrifying bacteria and the photosynthetic bacteria to a logarithmic phase, and mixing the bacteria liquids in proportion to obtain a mixed bacteria liquid. According to the mass ratio, the bacillus subtilis: nitrifying bacteria: denitrifying bacteria: the photosynthetic bacteria is 3:4:2: 1;
wherein, the step of culturing each strain comprises the following steps:
(1) bacillus subtilis
a1. Preparing a seed solution: inoculating the purified single bacillus subtilis colony into a liquid culture medium, and culturing at 37 ℃ at 200r/min for 18h to obtain a seed solution; wherein the culture medium is a mixed solution prepared according to the following proportion: 10g/L of tryptone, 5g/L of yeast extract powder, 10g/L of sodium chloride, 7.0-7.5 of pH value and 20min of sterilization at 121 ℃;
b1. fermentation production: inoculating the seed solution prepared in the step a1 into a fermentation medium according to the volume ratio of 5%, wherein the composition of the fermentation medium is the same as that of the liquid medium of the seed solution in the step a1, the culture conditions are the same as those in the step a1, and the fermentation is stopped when the spore yield reaches more than 90%, so as to obtain a bacillus subtilis liquid;
(2) nitrifying bacteria
a2. Preparing a seed solution: inoculating the purified nitrifying bacteria single colony into a liquid culture medium, and culturing at 30 ℃ at 200r/min for 72h to obtain seed liquid; wherein the culture medium is a mixed solution prepared according to the following proportion: 1g/L of ammonium sulfate, 1g/L of dipotassium hydrogen phosphate, 0.3g/L of sodium chloride, 0.3g/L of magnesium sulfate, 0.03g/L of ferrous sulfate, 7.5-8.0 of pH value and 20min of sterilization at 121 ℃;
b2. fermentation production: inoculating the seed solution prepared in the step a2 into a fermentation medium according to the volume ratio of 5%, wherein the fermentation medium is the same as the liquid medium of the seed solution in the step a2, the culture conditions are the same as the culture conditions in the step a2, and fermenting for 72 hours to obtain nitrobacteria liquid;
(3) denitrifying bacteria
a3. Preparing a seed solution: inoculating the purified single colony of the denitrifying bacteria into a liquid culture medium, and culturing at 30 ℃ and 100r/min for 24 hours to obtain seed liquid; wherein the liquid culture medium is a mixed solution prepared according to the following proportion: 2g/L of sodium acetate, 1g/L of sodium nitrate, 0.5g/L of dipotassium phosphate, 0.2g/L of magnesium sulfate, 2mL/L of trace elements and 6.8-7.2 of pH value, and sterilizing at 121 ℃ for 20 min; the trace elements are: 2.06g/L of EDTA, 1.54g/L of ferrous sulfate, 0.2g/L of manganese chloride, 0.1g/L of zinc sulfate, 0.02g/L of copper sulfate, 0.1g/L of sodium manganate, 2mg/L of cobalt chloride and the pH value of 6.8-7.2;
b3. fermentation production: inoculating the seed liquid prepared in the step a3 into a fermentation medium according to the volume ratio of 5%, wherein the composition of the fermentation medium is the same as that of the liquid medium of the seed liquid in the step a3, the culture conditions are the same as those in the step a3, and fermenting for 24 hours to obtain a denitrifying bacteria liquid;
(4) photosynthetic bacteria
a4. Preparing a seed solution: inoculating the purified single photosynthetic bacteria colony into a liquid culture medium, and culturing at 30 ℃, 200r/min and illumination intensity of 30001 x for 72 hours to obtain a seed solution; wherein the liquid culture medium is a mixed solution prepared according to the following proportion: 1g/L of ammonium chloride, 3.5g/L of sodium acetate, 0.1g/L of magnesium chloride, 0.1g/L of calcium chloride, 0.6g/L of monopotassium phosphate, 0.4g/L of dipotassium phosphate, 0.1g/L of yeast extract, 7.5-8.0 of pH value and 20min of sterilization at 121 ℃;
b4. fermentation production: inoculating the seed solution prepared in the step a4 into a fermentation medium according to the volume ratio of 5%, wherein the fermentation medium is the same as the liquid medium in the seed solution in the step a4, the culture conditions are the same as the culture conditions in the step a4, and fermenting for 72 hours to obtain a photosynthetic bacteria liquid;
s3, carrying out centrifugal concentration (2300r/min, 15min) on the mixed bacteria liquid with a certain volume, discarding supernatant, and uniformly mixing the concentrated liquid with pre-sterilized polyvinyl alcohol and sodium alginate solution (shaking table oscillation for 30min) to form mixed liquid of polyvinyl alcohol-sodium alginate and compound bacteria;
s4, dissolving the intraspecific signal molecules in distilled water, adding the interspecific signal molecules, and uniformly mixing; wherein: the intraspecific signal molecule adopts acylated homoserine lactone, specifically 2.5mgC4-HSL, 1mgC6-HSL, 2.5mgC12-HSL, 5mg 3OC8-HSL and 2.5mg 3OC 12-HSL; an interspecific signal molecule, namely AI-2, wherein the AI-2 quorum sensing signal molecule is generated by Vibrio harveyi BB170, and 10mL of a centrifugal supernatant liquid of the Vibrio harveyi BB170 can be taken in the embodiment of the invention;
s5, mixing the solution in the S4 with the mixed solution obtained in the S3, injecting the mixture into a disposable syringe, and slowly dripping the mixture into a 4% calcium chloride solution (20 cm away from the liquid level) while shaking. And (3) crosslinking the formed gel particles at 4 ℃ for 24 hours to obtain immobilized particles with the particle size of 2-4 mm.
And S6, after the gelling process is finished, washing the immobilized beads for 3 times by using normal saline, transferring and storing to obtain the slow-release microbial inoculum particles.
In the embodiment of the invention, the floating block carrier can be open-cell polyurethane foam. The prepared slow-release bacterium particles are uniformly dispersed and fixed on the upper surface and the lower surface of the disc-shaped soft polyurethane foam in an extruding mode, so that the functionalized floating block 3 is obtained, the functionalized floating block 3 can slowly and continuously release various functional microorganisms in a water body and promote signal molecules to quickly and stably form a biological membrane, and meanwhile, a microenvironment can be provided for various reactions.
The functional floating quarto can set up to a plurality ofly, specifically can confirm according to the required length of river pollution condition and artifical pasture and water, and the distance between the adjacent functional floating block 3 can set up to 0.5 ~ 1m, and for improving purification efficiency, the distance between the adjacent functional floating block 3 sets up 0.5 m.
The functionalized substrate 4 includes slow release oxygen particles and a substrate carrier for carrying the slow release oxygen particles. The substrate carrier can be a composite carrier of biological carbon and polyurethane; the slow-release oxygen particles take calcium peroxide as an oxygen release agent, polyhydroxy fatty acid ester as an embedding agent, and the mass ratio of the polyhydroxy fatty acid ester to the calcium peroxide is 1-3: 1. The preparation method of the oxygen-release-retarding particles comprises the following steps:
under anhydrous condition, polyhydroxy fatty acid ester is used as embedding medium, and methylene dichloride is used as dissolving medium. 10g of polyhydroxyalkanoate was added to 500mL of methylene chloride, and the mixture was dissolved in a horizontal magnetic stirrer at 150 r/min. After the calcium peroxide is completely dissolved in dichloromethane, 10g of calcium peroxide is added, and the mixture is continuously stirred uniformly to enable the calcium peroxide to be in a completely uniform suspension state. And extruding the mixture into balls after the dichloromethane gradually volatilizes and placing the balls in a fume hood overnight to completely volatilize the dichloromethane, and finally obtaining spherical slow-release oxygen particles with the average particle size of 8 mm.
The preparation steps of the biochar-supported functionalized substrate 4 in the embodiment are as follows:
s1, cutting loofah sponge into pieces with the volume of about 1cm3Small pieces of (2); in the atmosphere of nitrogen (1L/min), the temperature is raised to 500 ℃ at the heating rate of 10 ℃/min, and the carbonization time is 2h, thus obtaining the three-dimensional spongy biochar.
As shown in FIG. 2, the prepared biochar has a three-dimensional porous structure and micron-scale folds, and the specific surface area is large, so that the adsorption of pollutants is facilitated.
S2, blending polyether polyol and biochar for 90s, starting at a stirrer rotating speed of 200r/min, and increasing the rotating speed to 800r/min after 30 s; then adding corresponding MDI according to the mixing ratio of 1:1.8 of the polyol to the diphenylmethane diisocyanate (MDI), stirring for 30s, and the rotating speed of a stirrer is 800 r/min; quickly introducing the stirred material liquid into a mold, and curing in an oven at 65 +/-2 ℃ for 15 min; taking out and cooling.
S3, uniformly dispersing and fixing the prepared slow-release oxygen particles in the biochar-loaded polyurethane foam in an extruding mode to serve as a functional substrate 4. The slow-release oxygen-enriched water-saving fertilizer can slowly and continuously release oxygen in water, improve the anoxic environment of bottom mud and provide necessary oxygen for aerobic microorganisms attached to aquatic weed fibers.
The oxygen releasing agent, embedding agent and biochar of the present invention are not limited to the specific examples described above. For example, the oxygen-releasing particles may alternatively be replaced by magnesium oxide, the embedding agent may alternatively be replaced by polyvinyl alcohol, and the biochar may alternatively be replaced by activated carbon.
Example two
The embodiment of the invention applies the artificial aquatic weeds in the embodiment to ecological restoration of the river channel, and the artificial aquatic weeds are directly put into eutrophicated and black and odorous urban river channels to purify water quality. It should be understood that the length and the throwing density of the artificial aquatic plants can be determined according to the pollution degree of the river channel. As shown in fig. 3 to fig. 6, the artificial aquatic weed described in the first embodiment is applied to experimental data obtained by ecological restoration of a river channel for 15 days. The artificial aquatic weeds put in a river course of a certain 300 square meters and 2 meters deep city in Nanjing are used as an experimental group, and the artificial aquatic weeds put in a laboratory are used as a control group. Wherein, fig. 3, fig. 4, fig. 5 are the time-varying curve diagrams of the dissolved oxygen, ammonia nitrogen, and total phosphorus in example two, respectively. As can be seen from the figure, in the experimental period of 15day, the dissolved oxygen content measured from the sampling section of the black and odorous river channel added with the artificial aquatic weed is obviously and continuously increased compared with that of a blank control group, the concentrations of ammonia nitrogen and total phosphorus are continuously reduced, the water quality of the sampling point of the river channel is improved from the fifth category to the fourth category (according to the quality standard GB3838-2002 of the surface water environment), the transparency of river water is obviously increased, and the odor is also removed.
FIG. 6 is a cladogram of LefSe analysis of artificial aquatic weeds in river ecological restoration. Wherein, the innermost circle represents the difference of the phylum level, the circles sequentially outwards represent class, order, family and genus, the diameter of the small circle is in direct proportion to the relative abundance of the represented class group, the yellow node represents the species without obvious difference in the two groups, and the red node and the green node respectively represent the species playing important roles in the experimental group and the control group after the experiment 15 d. At the phylum level, Firmicutes in the control group and Proteobacteria (Proteobacteria) and actinomycetes (Actinobacteria) in the experimental group play an important role. On the genus level, Bacillus (Bacillus) in a control group and cuprianidus (Cupriavidus) in an experimental group play an important role. The experimental group and the control group have obvious difference in the composition of the bacterial community, and the artificial aquatic weeds can promote the formation of a stable microbial community in a substrate environment, so that the composition of the bacterial community in the experimental group is consistent with that of a normal river, and the self-cleaning capacity of the river is improved.
The above description is only a preferred embodiment of the present invention, and the present invention is not limited to the content of the embodiment. It will be apparent to those skilled in the art that various changes and modifications can be made within the technical scope of the present invention, and any changes and modifications made are within the protective scope of the present invention.

Claims (10)

1. An artificial float grass is characterized by comprising a middle rope, a float, float grass fibers, a functional float block, a functional substrate and a heavy weight, wherein the float, the float grass fibers, the functional float block, the functional substrate and the heavy weight are connected in series by the middle rope; the functionalized floating block comprises slow-release microbial inoculum particles and a floating block carrier for bearing the slow-release microbial inoculum particles; the slow-release microbial inoculum particles adopt a composite microbial inoculum and are filled with signal molecules; the functionalized substrate comprises slow-release oxygen particles and a substrate carrier for carrying the slow-release oxygen particles.
2. The artificial aquatic weed according to claim 1, wherein the functionalized floating blocks are provided in plurality, and the interval between adjacent functionalized floating blocks is 0.5-1 m.
3. The artificial waterweed as recited in claim 1, wherein the complex microbial inoculum comprises bacillus subtilis, nitrobacteria, denitrifying bacteria and photosynthetic bacteria.
4. The artificial aquatic weed of claim 1 wherein said floe carrier is an open-cell polyurethane foam carrier.
5. The artificial waterweed as claimed in claim 1, wherein the signal molecule comprises acylated homoserine lactone and AI-2.
6. The artificial aquatic weed according to claim 1, wherein the slow-release microbial inoculum particles take sodium alginate and polyvinyl alcohol as composite embedding materials, and the dosage ratio of the sodium alginate to the polyvinyl alcohol is 1-3: 1; with boric acid and CaCl2The mixed solution of (2) is a cross-linking agent.
7. The artificial aquatic weed according to claim 1, wherein the substrate carrier is a composite carrier of biochar and polyurethane.
8. The artificial waterweed as claimed in claim 1, wherein the oxygen-releasing particles take calcium peroxide as an oxygen-releasing agent, polyhydroxyalkanoate as an embedding agent, and the mass ratio of polyhydroxyalkanoate to calcium peroxide is 1-3: 1.
9. The artificial waterweed as claimed in claim 1, wherein the waterweed fibers are made of polypropylene and vinylon and are uniformly fixed on the middle rope in the form of annular filament group.
10. The use of the artificial aquatic weed of any one of claims 1-9 in river ecological restoration.
CN202010452659.6A 2020-05-26 2020-05-26 Artificial aquatic weed and application thereof Pending CN111646640A (en)

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Application publication date: 20200911