CN111644213A - Fluid control device and fluid control method - Google Patents
Fluid control device and fluid control method Download PDFInfo
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- CN111644213A CN111644213A CN202010447542.9A CN202010447542A CN111644213A CN 111644213 A CN111644213 A CN 111644213A CN 202010447542 A CN202010447542 A CN 202010447542A CN 111644213 A CN111644213 A CN 111644213A
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
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Abstract
The invention relates to a fluid control device and a fluid control method, which are used for realizing the functions of quantitative micro-fluid mixing and transferring or one-step fluid mixing and transferring and comprise the following steps: a cartridge having a control chamber/a mixing chamber, a reagent chamber and a recovery chamber formed thereon; a lower chip on which a quantitative pool or a fluid channel is formed; and the elastic membrane is used for bonding the lower chip on the bottom of the cartridge. The control chamber/the blending chamber, the reagent chamber and the recovery chamber form a first one-way valve structure which can only go out but not go in or a second one-way valve structure which can only go in but not go out through the elastic membrane and the quantitative pool or the fluid channel. The fluid control device provided by the invention can be independent of the control and operation of any external instrument, can complete the fluid control, trace fluid quantification and one-step liquid mixing and transferring operation only by simple pressure application actions for a plurality of times, and can realize the totally closed system, thereby effectively avoiding the problems of pollutant leakage, aerosol pollution and the like.
Description
Technical Field
The present invention relates to a fluid manipulation device, and more particularly, to a fluid manipulation device and a fluid control method capable of easily and stably manipulating a trace amount of fluid.
Background
The micro-fluidic chip realizes the precise control of micro-fluid by processing the structure of a micro pump, a micro valve, a micro pipeline, a microelectrode and the like on a micro-scale chip, can transfer biochemical analysis reaction which can only be operated by professional personnel in a laboratory to the chip for automatic realization, can be used for cell screening and capturing, cell culture, protein detection, nucleic acid extraction, nucleic acid amplification, capillary electrophoresis and the like through a specially designed micro-fluidic chip, and has huge application prospect in the fields of medical treatment, environmental protection, scientific research, criminal investigation and the like.
Compare in traditional use pipette, centrifuging tube and carry out experimental operation, microfluidic chip integrates, the automation aspect has the advantage, nevertheless receives the restriction of fluid control mode, and microfluidic chip still faces following problem: (1) the fluid control mode is complex, and the fluid control on the microfluidic chip usually needs to be externally connected with an air pressure source such as an injection pump, an air pump and the like or a centrifuge is used for providing fluid driving force, so that the volume and the cost of the whole system are greatly increased; or surface modification and capillary force are used for realizing fluid self-driving, but the stability is insufficient, so that the application scene of the microfluidic system is limited; (2) the quantification of trace fluid is difficult, many steps in a biochemical analysis experiment need to accurately absorb microliter-level liquid by using a pipette, the liquid is used for reaction system configuration and the like, but the step is difficult to realize on a microfluidic chip, and a complex valve structure is often required to be designed, or the precision of the whole system is sacrificed; (3) the problem of fluid mixing, which involves multiple liquid mixing steps in the biochemical reaction process, causes more difficulties for the design of the microfluidic chip; (4) the problem of system closure, the biochemical reaction process needs to be carried out in a centrifugal tube in a closed manner, for example, nucleic acid amplification is taken as an example, as the nucleic acid needs to be heated and amplified exponentially in the amplification process, aerosol pollution is easily generated, and false positive is possibly caused; therefore, the internal fluid space of the micro-fluidic chip needs to be totally closed, and meanwhile, the interface for controlling the fluid between the chip and the outside is considered.
Disclosure of Invention
In view of the above problems, an object of the present invention is to provide a fluid control device and a fluid control method capable of simply and stably controlling a micro-fluid, which can realize simple and stable fluid control, micro-fluid quantification, and one-step liquid mixing and transfer by means of a micro-fluidic chip, and can realize the totally-enclosed system.
In order to achieve the purpose, the invention adopts the following technical scheme: a fluid manipulation device for performing a micro-fluid quantification function, the fluid manipulation device comprising: the kit comprises a card box, a control chamber, a reagent chamber and a recovery chamber, wherein the card box is provided with the control chamber, the reagent chamber and the recovery chamber, and the bottoms of the control chamber, the reagent chamber and the recovery chamber are provided with openings; a lower chip on which a quantitative pool is formed; the elastic membrane is used for bonding the lower layer chip at the bottom of the card box, and the elastic membrane is provided with through holes with the same number as the number of the openings at the bottom of the card box, wherein the opening of the control chamber is superposed with the through holes on the elastic membrane so as to ensure that the control chamber is communicated with the quantitative pool; the openings of the reagent chamber and the recovery chamber are not coincident with the through hole on the elastic membrane, so that a first one-way valve structure which can only go out and cannot go in is formed between the opening of the reagent chamber and the quantitative pool, and a second one-way valve structure which can only go in and cannot go out is formed between the opening of the recovery chamber and the quantitative pool; and the hydrophobic membrane is arranged at the through hole of the elastic membrane between the control chamber and the quantitative pool.
The fluid control device, preferably, the elastic membrane is a double-sided adhesive tape substrate, and is subjected to local de-bonding treatment.
The fluid control device, preferably, the control chamber is controlled by a push rod piston structure, an external air pump or an injection pump; a liquid reaction reagent is pre-stored in the reagent chamber, and a color developing agent or a test strip for detection is pre-stored in the recovery chamber; the volume of the quantitative pool is 20-100 mu L, and dry powder reaction reagents are pre-stored in the quantitative pool.
A fluid control method based on the fluid control device comprises the following steps:
1) applying negative pressure to the control chamber, opening a first one-way valve structure between the reagent chamber and the quantitative pool under the action of the negative pressure, and allowing the liquid in the reagent chamber to flow into the quantitative pool but not to pass through the hydrophobic membrane to enter the control chamber; meanwhile, a second one-way valve structure between the recovery chamber and the quantitative pool is kept closed under the action of negative pressure;
2) the positive pressure is applied to the control chamber, the first one-way valve structure between the reagent chamber and the quantitative pool is closed under the action of the positive pressure, the second one-way valve structure between the recovery chamber and the quantitative pool is opened under the action of the positive pressure, at the moment, the liquid in the quantitative pool flows into the recovery chamber, and the volume of the liquid flowing into the recovery chamber is equal to that of the quantitative pool.
A fluid control device for realizing a one-step fluid blending transfer function comprises: the reagent box comprises a card box body, a mixing chamber and a recovery chamber, wherein a reagent chamber, a mixing chamber and a recovery chamber are formed on the card box body, openings are formed at the bottoms of the reagent chamber and the recovery chamber, the mixing chamber is a closed chamber, and an inlet and an outlet are formed at the bottom of the mixing chamber; the lower chip is provided with at least two fluid pipelines which are not communicated; elastic membrane, be used for with lower floor's chip bonding is in the bottom of card box, elastic membrane is last seted up with the same through-hole of card box bottom opening figure, just reagent cavity, mixing chamber and recovery cavity with through-hole on the elastic membrane is all coincide, so that the opening of reagent cavity and one of them between the fluid pipeline and the export and another of mixing cavity form between the fluid pipeline can only go out the first check valve structure that can not advance, mixing chamber's import and one of them between the fluid pipeline and retrieve the opening and another of cavity form between the fluid pipeline can only go into the second check valve structure that can not go out.
The fluid control device, preferably the top of card box is provided with the gasbag structure, be used for right the top of card box carries out totally closed, and removes outside the mixing chamber, reagent chamber and recovery chamber all through the air guide structure with the gasbag structure is linked together.
The fluid manipulation device, preferably, the balloon structure includes, but is not limited to, a sealing tube, a sealing membrane, and an elastic balloon; the air guide structure includes, but is not limited to, an air guide hole, an air guide groove and an air guide channel.
The fluid control device, preferably, the elastic membrane is a double-sided adhesive tape substrate, and is subjected to local de-bonding treatment.
Fluid control device, preferably, there is liquid reaction reagent in reagent cavity and the mixing cavity in advance, there is color development agent or test paper strip of detection usefulness in advance in the recovery cavity, there is dry powder form reaction reagent in advance in the fluid pipeline.
A fluid control method based on the fluid control device comprises the following steps:
1) presetting a reagent in a reagent chamber, and presetting a reagent in a mixing chamber;
2) applying positive pressure to the reagent chamber, opening a first one-way valve structure and a second one-way valve structure between the opening of the reagent chamber and the inlet of the blending chamber under the action of the positive pressure, and allowing the reagent to enter the blending chamber through one of the fluid pipelines and to contact with the reagent in the blending chamber; because the air in the blending chamber has compressibility, the first one-way valve between the outlet of the blending chamber and the other fluid pipeline is not opened;
3) continuously applying positive pressure to the reagent chamber, allowing air to enter the mixing chamber, and rapidly mixing the reagent and the reagent under the action of bubble oscillation;
4) when the internal air pressure of the blending cavity reaches a critical value, the first one-way valve structure and the second one-way valve structure between the outlet of the blending cavity and the opening of the recovery cavity are both opened under the positive pressure effect, and the blended reagent enters the recovery cavity through another fluid pipeline.
Due to the adoption of the technical scheme, the invention has the following advantages: 1. the fluid control device provided by the invention does not depend on the control and operation of any external instrument, and can finish the quantitative and uniform mixing of the reagent and the reagent transfer operation only by a plurality of simple pressure applying actions. 2. The invention realizes the fluid limit in the process of pressing action by using the hydrophobic membrane, realizes the accurate quantification of microliter-level liquid by the hydrophobic membrane and the quantification pool on the lower chip, and solves the problem of difficult quantification on the microfluidic chip. 3. When the one-step method for uniformly mixing and transferring the fluid is realized, the liquid and the gas are sequentially introduced into the closed reagent chamber, the uniform mixing of different reagents is realized when the liquid is introduced, the gas compression and the liquid pumping are realized when the gas is introduced, the two steps of uniformly mixing and transferring the reagents can be realized by applying positive pressure in one step, and the operation of controlling the fluid of the microfluidic chip is simplified. 4. The fluid control device provided by the invention realizes the communication and the sealing of the reaction chamber through the air guide hole and the sealing film, and can realize the full sealing of the whole reaction system, thereby effectively avoiding the problems of pollutant leakage, aerosol pollution and the like.
Drawings
FIG. 1 is a schematic structural diagram of a fluid control device according to an embodiment of the present invention;
FIGS. 2(a) and 2(b) are schematic diagrams illustrating the quantification of a micro-fluid according to an embodiment of the present invention;
FIG. 3 is a schematic structural diagram of a fluid control device according to a second embodiment of the present invention;
fig. 4(a) to fig. 4(d) are schematic diagrams illustrating a fluid blending transfer in a one-step method according to a second embodiment of the present invention;
FIG. 5 is a schematic diagram of a fully enclosed fluid management device according to the present invention;
fig. 6 is a schematic diagram of a fully enclosed fluid management device of the present invention.
Detailed Description
The preferred embodiments of the present invention will be described in detail below with reference to the accompanying drawings so that the objects, features and advantages of the invention can be more clearly understood. It should be understood that the embodiments shown in the drawings are not intended to limit the scope of the present invention, but are merely intended to illustrate the spirit of the technical solution of the present invention.
The first embodiment is as follows:
fig. 1 and fig. 2 show a fluid handling device capable of performing a micro fluid metering function according to an embodiment of the present invention, the fluid handling device includes: a cartridge 1 on which a control chamber 6, a reagent chamber 8 and a recovery chamber 10 are formed, and the bottoms of the control chamber 6, the reagent chamber 8 and the recovery chamber 10 are formed with an opening; a lower chip 2 on which a quantitative cell 11 is formed; the elastic membrane 3 is used for bonding the lower layer chip 2 at the bottom of the card box 1, and the elastic membrane 3 is provided with through holes with the same number as the openings at the bottom of the card box 1, wherein the opening of the control chamber 6 is superposed with the through holes on the elastic membrane 3, so that the control chamber 6 is communicated with the quantitative pool 11; the openings of the reagent chamber 8 and the recovery chamber 10 are not overlapped with the through hole on the elastic membrane 3, so that a first one-way valve structure 15 which can only go out and cannot go in is formed between the opening of the reagent chamber 8 and the quantitative pool 11, and a second one-way valve structure 18 which can only go in and cannot go out is formed between the opening of the recovery chamber 10 and the quantitative pool 11; a hydrophobic membrane 4, arranged at the through hole of the elastic membrane 3 between the control chamber 6 and the dosing reservoir 11, the hydrophobic membrane 4 being permeable for air but impermeable for liquid.
In the above embodiment, preferably, the control chamber 6 may be controlled by a push rod piston structure, an external air pump or an injection pump, or other air pressure sources; a liquid reaction reagent is pre-stored in the reagent chamber 8, and a color developing agent or a test strip for detection is pre-stored in the recovery chamber 10; the volume of the quantitative pool 11 is 20-100 μ L, and dry powder-shaped reaction reagents can be pre-stored in the quantitative pool.
In the above embodiment, preferably, the elastic film 3 can be made of a double-sided adhesive base material, and can perform both the check valve function and the bonding of the cartridge 1 and the lower chip 2 through a partial de-sticking treatment.
Based on the fluid control device provided by the above embodiment, the invention further provides a fluid control method based on the fluid control device, which includes the following steps:
1) applying negative pressure to the control chamber 6, opening a first one-way valve structure 15 between the reagent chamber 8 and the quantitative pool 11 under the action of the negative pressure, and enabling the liquid in the reagent chamber 8 to flow into the quantitative pool 11 but not to pass through the hydrophobic membrane 4 to enter the control chamber 6; while the second one-way valve structure 18 between the recovery chamber 10 and the dosing reservoir 11 remains closed under negative pressure (as shown in figure 2 (a)).
2) When positive pressure is applied to the control chamber 6, the first one-way valve structure 15 between the reagent chamber 8 and the quantitative cell 11 is closed by the positive pressure, and the second one-way valve structure 18 between the recovery chamber 10 and the quantitative cell 11 is opened by the positive pressure, the liquid in the quantitative cell 11 flows into the recovery chamber 10, and the volume of the liquid flowing into the recovery chamber 10 is equal to the volume of the quantitative cell 11 (as shown in fig. 2 (b)).
Example two:
fig. 3 and 4 show a fluid handling device capable of implementing a one-step fluid blending transfer function according to a second embodiment of the present invention, the fluid handling device includes: the card box 1 is provided with a reagent chamber 8, a blending chamber 9 and a recovery chamber 10, the bottoms of the reagent chamber 8 and the recovery chamber 10 are both provided with an opening, the blending chamber 9 is a closed chamber, and the bottom of the blending chamber is provided with an inlet and an outlet; a lower chip 2 on which at least two fluid channels 19 that are not communicated are formed; the elastic membrane 3 is used for bonding the lower chip 2 at the bottom of the card box 1, through holes with the same number as the openings at the bottom of the card box 1 are formed in the elastic membrane 3, the reagent chamber 8, the blending chamber 9, the recovery chamber 10 and the through holes in the elastic membrane 3 are not overlapped, so that a first one-way valve structure 15 which can only go in and out is formed between the opening of the reagent chamber 8 and one of the fluid pipelines 19 and between the outlet of the blending chamber 9 and the other fluid pipeline 19, and a second one-way valve structure 18 which can only go in and out is formed between the inlet of the blending chamber 9 and one of the fluid pipelines 19 and between the opening of the recovery chamber 10 and the other fluid pipeline 19.
In the above embodiment, preferably, as shown in fig. 5 and 6, a balloon structure 20 is provided on the top of the cartridge 1 for totally closing the top of the cartridge 1, and the reagent chamber 8 and the recovery chamber 10 are communicated with the balloon structure 20 through the air guide structure 14 except the blending chamber 9.
In the above embodiments, the bladder structure 20 preferably includes, but is not limited to, a sealing tube, a sealing film, an elastic bladder, and the like.
In the above embodiments, the air guide structure 14 preferably includes, but is not limited to, an air guide hole, an air guide groove, an air guide duct, and the like.
In the above embodiment, preferably, the reagent chamber 8 and the mixing chamber 9 are pre-stored with liquid reaction reagents, and the recovery chamber 10 is pre-stored with a color developing agent or a test strip for detection; the fluid conduit 19 may have pre-stored therein a reactive reagent in the form of dry powder.
In the above embodiment, preferably, the elastic film 3 can be made of a double-sided adhesive base material, and can perform both the check valve function and the bonding of the cartridge 1 and the lower chip 2 through a partial de-sticking treatment.
Based on the fluid control device provided by the above embodiment, the present invention further provides a fluid control method of the fluid control device, including the following steps:
1) the reagent a is preliminarily stored in the reagent chamber 8, and the reagent B is preliminarily stored in the kneading chamber 9 (see fig. 4 (a)).
2) Applying positive pressure to the reagent chamber 8, opening both the first one-way valve structure 15 and the second one-way valve structure 18 between the opening of the reagent chamber 8 and the inlet of the blending chamber 9 under the action of the positive pressure, and allowing the reagent A to enter the blending chamber 9 through one of the fluid pipelines 19 to contact with the reagent B in the blending chamber 9; due to the compressibility of the air within the blending chamber 9, the first one-way valve 15 between the outlet of the blending chamber 9 and the further fluid conduit 19 is not opened (as shown in fig. 4 (b)).
3) The positive pressure is continuously applied to the reagent chamber 8, air enters the blending chamber 9, and the reagent A and the reagent B are rapidly blended under the action of the bubble oscillation (as shown in fig. 4 (c)).
4) When the internal air pressure of the blending chamber 9 reaches a critical value, the first one-way valve structure 15 and the second one-way valve structure 18 between the outlet of the blending chamber 9 and the opening of the recovery chamber 10 are both opened under the positive pressure, and the blended reagent enters the recovery chamber 10 through another fluid pipeline 19 (as shown in fig. 4 (d)).
It is noted that in the above embodiments, the fluid handling device is shaped like a tape cartridge, but the fluid handling device may be modified for specific applications without departing from the inventive concept, such as the following structural modifications: the shape of the card box 1 can be adjusted according to the processing mode and the functional requirements, and can be combined into a cuboid, a cylinder, a circular truncated cone and the like; the arrangement sequence of the chambers on the card box 1 can be correspondingly adjusted according to the functional requirements; the arrangement of the quantitative pool 11 or the fluid channel 19 on the lower chip 2 can be adjusted correspondingly according to the arrangement sequence of the chambers on the card box 1; the number of the control chambers 6 or the blending chambers 9 on the cartridge 1 can be adjusted according to requirements, and the number of the quantitative cells 11 or the fluid channels 19 on the lower chip 2 can be adjusted according to requirements.
Finally, it should be noted that: the above examples are only intended to illustrate the technical solution of the present invention, but not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some technical features may be equivalently replaced; and such modifications or substitutions do not depart from the spirit and scope of the corresponding technical solutions of the embodiments of the present invention.
Claims (10)
1. A fluid manipulation device for performing a micro-fluid quantification function, the fluid manipulation device comprising:
the kit comprises a card box (1), a control chamber (6), a reagent chamber (8) and a recovery chamber (10) are formed on the card box, and openings are formed at the bottoms of the control chamber (6), the reagent chamber (8) and the recovery chamber (10);
a lower chip (2) on which a quantitative cell (11) is formed;
the elastic membrane (3) is used for bonding the lower chip (2) at the bottom of the card box (1), and through holes with the same number as the openings at the bottom of the card box (1) are formed in the elastic membrane (3), wherein the openings of the control chamber (6) are overlapped with the through holes in the elastic membrane (3) so that the control chamber (6) is communicated with the quantitative pool (11); the openings of the reagent chamber (8) and the recovery chamber (10) are not coincident with the through hole on the elastic membrane (3), so that a first one-way valve structure (15) which can only be accessed and can not be accessed is formed between the opening of the reagent chamber (8) and the quantitative pool (11), and a second one-way valve structure (18) which can only be accessed and can not be accessed is formed between the opening of the recovery chamber (10) and the quantitative pool (11);
a hydrophobic membrane (4) arranged at the through hole of the elastic membrane (3) between the control chamber (6) and the quantification pool (11).
2. The fluid handling device according to claim 1, wherein the flexible membrane (3) is a double-sided adhesive substrate, and is partially detackified.
3. The fluid handling device according to claim 1, wherein the control chamber (6) is controlled by a push rod piston structure, an external air pump or an injection pump; a liquid reaction reagent is pre-stored in the reagent chamber (8), and a color developing agent or a test strip for detection is pre-stored in the recovery chamber (10); the volume of the quantitative pool (11) is 20-100 mu L, and dry powder reaction reagents are pre-stored in the quantitative pool.
4. A fluid control method based on the fluid control device according to any one of claims 1 to 3, comprising the steps of:
1) applying negative pressure to the control chamber (6), opening a first one-way valve structure (15) between the reagent chamber (8) and the quantitative pool (11) under the action of the negative pressure, and enabling liquid in the reagent chamber (8) to flow into the quantitative pool (11) but not to pass through the hydrophobic membrane (4) to enter the control chamber (6); meanwhile, a second one-way valve structure (18) between the recovery chamber (10) and the quantitative pool (11) is kept closed under the action of negative pressure;
2) the positive pressure is applied to the control chamber (6), the first one-way valve structure (15) between the reagent chamber (8) and the quantitative pool (11) is closed under the action of the positive pressure, the second one-way valve structure (18) between the recovery chamber (10) and the quantitative pool (11) is opened under the action of the positive pressure, at the moment, the liquid in the quantitative pool (11) flows into the recovery chamber (10), and the volume of the liquid flowing into the recovery chamber (10) is equal to the volume of the quantitative pool (11).
5. A fluid control device is used for realizing the function of uniformly mixing and transferring fluid in a one-step method, and is characterized by comprising:
the reagent box comprises a card box (1), a reagent chamber (8), a blending chamber (9) and a recovery chamber (10), wherein the reagent chamber (8) and the recovery chamber (10) are respectively provided with an opening at the bottom, the blending chamber (9) is a closed chamber, and an inlet and an outlet are formed at the bottom of the blending chamber;
a lower chip (2) on which at least two fluid channels (19) that are not connected are formed;
an elastic membrane (3) for bonding the lower chip (2) to the bottom of the cartridge (1), the elastic membrane (3) is provided with through holes with the same number as the openings at the bottom of the card box (1), the reagent chamber (8), the blending chamber (9) and the recovery chamber (10) are not overlapped with the through holes on the elastic membrane (3), so that a first one-way valve structure (15) which can only be out and not be in is formed between the opening of the reagent chamber (8) and one of the fluid pipelines (19) and between the outlet of the blending chamber (9) and the other fluid pipeline (19), and second one-way valve structures (18) which can only enter and cannot exit are formed between the inlet of the blending cavity (9) and one of the fluid pipelines (19) and between the opening of the recovery cavity (10) and the other fluid pipeline (19).
6. The fluid control device according to claim 5, characterized in that an air bag structure (20) is arranged on the top of the cartridge (1) for totally closing the top of the cartridge (1), and the reagent chamber (8) and the recovery chamber (10) are communicated with the air bag structure (20) through an air guide structure (14) except the blending chamber (9).
7. The fluid manipulation device of claim 6, wherein the balloon structure (20) comprises, but is not limited to, a sealing tube, a sealing membrane, and an elastic balloon; the air guide structure (14) includes, but is not limited to, an air guide hole, an air guide groove and an air guide duct.
8. The fluid handling device according to claim 5, wherein said elastic membrane (3) is a double-sided adhesive substrate, and is partially detackified.
9. The fluid control device according to claim 5, wherein liquid reaction reagents are pre-stored in the reagent chamber (8) and the mixing chamber (9), color-developing agents or test strips for detection are pre-stored in the recovery chamber (10), and dry powder reaction reagents are pre-stored in the fluid pipeline (19).
10. A fluid control method based on the fluid control device according to any one of claims 5 to 9, comprising the steps of:
1) presetting a reagent (A) in a reagent chamber (8) and presetting a reagent (B) in a blending chamber (9);
2) applying positive pressure to the reagent chamber (8), opening a first one-way valve structure (15) and a second one-way valve structure (18) between the opening of the reagent chamber (8) and the inlet of the blending chamber (9) under the action of the positive pressure, and enabling the reagent (A) to enter the blending chamber (9) through one of the fluid pipelines (19) to be in contact with the reagent (B) in the blending chamber (9); the first one-way valve (15) between the outlet of the homogenizing chamber (9) and the other fluid conduit (19) is not opened due to the compressibility of the air inside the homogenizing chamber (9);
3) continuously applying positive pressure to the reagent chamber (8), allowing air to enter the blending chamber (9), and rapidly blending the reagent (A) and the reagent (B) under the action of bubble oscillation;
4) when the internal air pressure of the blending cavity (9) reaches a critical value, the first one-way valve structure (15) and the second one-way valve structure (18) between the outlet of the blending cavity (9) and the opening of the recovery cavity (10) are opened under the action of positive pressure, and the blended reagent enters the recovery cavity (10) through another fluid pipeline (19).
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