CN111642338B - Method for promoting growth of banana seedlings by inoculating arbuscular mycorrhizal fungi - Google Patents
Method for promoting growth of banana seedlings by inoculating arbuscular mycorrhizal fungi Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/05—Fruit crops, e.g. strawberries, tomatoes or cucumbers
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
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Abstract
The invention discloses a method for promoting banana seedling growth by inoculating arbuscular mycorrhizal fungi. According to the invention, the mixed strain of the arbuscular mycorrhizal fungi (the sacculus mycorrhizal fungi and the cassava sacculus fungi are mixed according to the spore ratio of 1: 1) is inoculated to the root system of the banana, so that the mixed strain of the arbuscular mycorrhizal fungi is found to be used as a high-efficiency and safe biological fertilizer, has a remarkable improvement effect on the growth and development of banana seedlings, is used as a renewable and sustainable resource, and has better ecological economic benefit and environmental sustainable effect than other biological fertilizers. After the arbuscular mycorrhizal fungi mixed strain is inoculated for 3 months, the growth of bacteria, fungi, actinomycetes, azotobacter and ammonifying bacteria in the culture medium is promoted, the metabolic activity of the soil microbial community of the banana seedling is obviously optimized, and the growth of the banana tissue culture seedling is obviously promoted by a large amount of bacterial metabolic activity.
Description
Technical Field
The invention relates to the technical field of gardens, in particular to a method for promoting banana seedling growth by inoculating arbuscular mycorrhizal fungi.
Background
Bananas belong to Musa plants of Musaceae, Musa, are globally important food crops and tropical subtropical fruits and are mainly distributed in provinces (regions) of Guangdong, Guangxi, Hainan, Yunnan, Fujian and the like in China. In 2017, the banana planting area in China is 590 ten thousand mu, the harvesting area is 495 ten thousand mu, and the yield is 1250 ten thousand tons, so that the banana planting method is one of important economic crops for increasing the income of farmers in tropical regions. However, the traditional banana cultivation method depends on a large amount of chemical fertilizers, so that the problems of soil structure damage, soil hardening, reduction of organic matter content, low microbial community activity and the like of the banana garden are often caused, and the growth of bananas, particularly seedlings, is obviously influenced.
Arbuscular mycorrhizal fungi (AM fungi) have been of widespread interest as an important plant biocontrol bacterium. Mycorrhiza is an extremely common and important symbiotic phenomenon in nature, and can form symbiotic structures with various plants in farmland soil, accounting for 9% -55% of the total amount of soil microorganisms. The formation of most arbuscular mycorrhiza is beneficial to the nutrient absorption of plants in soil, promotes the growth of plants, improves the disease resistance of plants and the like. Therefore, it is necessary to develop a method for improving the growth of banana seedlings by inoculating arbuscular mycorrhizal fungi.
Disclosure of Invention
The first purpose of the invention is to provide a method for promoting the growth of banana seedlings by inoculating arbuscular mycorrhizal fungi. The method comprises the following steps: and inoculating the arbuscular mycorrhizal fungi mixed strain to the root system of the banana seedlings, wherein the arbuscular mycorrhizal fungi mixed strain comprises Glomus intraradices (Glomus intraradies) and Glomus manihot (Glomus manihot).
Preferably, the arbuscular mycorrhizal fungi mixed strain is prepared by mixing Glomus intraradices (Glomus intraradics) and Glomus manihot (Glomus manihot) according to a spore ratio of 1: 1.
The Glomus intraradices (Glomus intraradics) and Glomus manihot (Glomus manihot) are purchased from a germplasm resource library of the rhizopus chinensis, and are propagated for 16 weeks by virtue of Trifolium repens L and stored for later use at 4 ℃.
Preferably, the mixed strain of the arbuscular mycorrhizal fungi is inoculated to the root system of the banana seedling, and the specific steps are as follows: cleaning a culture medium in the banana seedlings by using clear water, soaking the banana seedlings in the mixed strain inoculation liquid of the arbuscular mycorrhizal fungi, and soaking the roots for a period of time; planting the soaked banana seedlings in nutrient soil, and uniformly pouring the rest inoculation liquid into the nutrient soil.
The banana seedlings are 5-leaf-old and consistent-growth-vigor Brazil banana (Musa acetate. AAA group cv. Cavendish) tissue culture seedlings.
The preparation steps of the arbuscular mycorrhizal fungi mixed strain inoculation liquid are as follows: respectively activating intraradicular Glomus (Glomus intraradics) and cassava Glomus (Glomus manihot) in a dark in an incubator at 25 ℃, then respectively preparing a spore suspension with the concentration of 4000 spores/mL by using purified water as a 100% standard concentration inoculation liquid, and mixing the 100% standard concentration inoculation liquid of the intraradicular Glomus and the 100% standard concentration inoculation liquid of the cassava Glomus according to the spore proportion of 1:1 to obtain the mixed strain inoculation liquid of the arbuscular mycorrhizal fungi.
The root soaking treatment is carried out for 30 min.
The nutrient soil is formed by mixing peat soil and coconut coir according to the volume ratio of 1: 2. Mixing peat soil and coconut coir uniformly, and filling into a nutrition cup with the size of 8cm multiplied by 12cm for later use.
The second purpose of the invention is to provide the application of the mixed strain of the arbuscular mycorrhizal fungi in promoting the growth and development of banana seedlings, wherein the mixed strain of the arbuscular mycorrhizal fungi is formed by mixing Glomus intraradices (Glomus intraradics) and Glomus manihot (Glomus manihot) according to the spore ratio of 1: 1.
The third purpose of the invention is to provide a microbial inoculum for promoting the growth and development of banana seedlings, wherein the microbial inoculum is prepared by mixing Glomus intraradices (Glomus intraradis) and Glomus manihot (Glomus manihot) according to a spore ratio of 1: 1.
Compared with the prior art, the invention has the following advantages and effects:
according to the invention, the mixed strain of the arbuscular mycorrhizal fungi (the sacculus mycorrhizal fungi and the cassava sacculus fungi are mixed according to the spore ratio of 1: 1) is inoculated to the root system of the banana seedling, so that the arbuscular mycorrhizal fungi mixed strain is found to be used as a high-efficiency and safe biological fertilizer, has a remarkable improvement effect on the growth and development of the banana seedling, is used as a renewable and sustainable resource, and has better ecological economic benefit and environmental sustainable effect than other biological fertilizers. After the arbuscular mycorrhizal fungi mixed strain is inoculated for 3 months, the growth of bacteria, fungi, actinomycetes, azotobacter and ammonifying bacteria in the culture medium is promoted, the metabolic activity of the soil microbial community of the banana seedling is obviously optimized, and the growth of the banana tissue culture seedling is obviously promoted by a large amount of bacterial metabolic activity. The inoculated arbuscular mycorrhizal fungi shows good application effect in banana cultivation and has wide prospect in cultivation and application of bananas and other plants.
Drawings
FIG. 1 shows the growth of bacteria in banana seedling culture substrates treated with different inoculated arbuscular mycorrhizal fungi.
FIG. 2 shows the growth of different inoculated arbuscular mycorrhizal fungi in banana seedling culture medium.
FIG. 3 shows the growth of actinomycetes in different inoculated arbuscular mycorrhizal fungi treated banana seedling culture substrates.
FIG. 4 shows the growth of nitrogen-fixing bacteria in banana seedling culture medium treated by different inoculated arbuscular mycorrhizal fungi.
FIG. 5 shows the growth of the ammoniated bacteria in the cultivation substrate of banana seedlings treated by different inoculated arbuscular mycorrhizal fungi.
Detailed Description
The following examples are further illustrative of the present invention and are not intended to be limiting thereof.
Example 1: effect of inoculation of arbuscular mycorrhizal fungi on Banana plant Biomass
Experiment setup 4 experimental groups: control (CK, no arbuscular mycorrhizal fungi inoculated), treatment 1(T1, 100% strength sacculus rhizogenes inoculated), treatment 2(T2, 100% strength sacculus cassava) and treatment 3(T3, 100% strength sacculus rhizogenes mixed with 100% strength sacculus cassava in a spore ratio of 1:1 and inoculated).
The specific steps of the treatment 3 are as follows: cleaning a culture medium in a banana tissue culture seedling (5-leaf-old Brazilian banana tissue culture seedling with consistent growth vigor) by using clear water, soaking the banana tissue culture seedling in an arbuscular mycorrhizal fungi mixed strain inoculation liquid (preparation: respectively activating sacculus rhizogenes and sacculus cassava in an incubator at 25 ℃ in the dark, then respectively preparing a spore suspension with the concentration of 4000 spores/mL by using purified water as a 100% standard concentration inoculation liquid, mixing the 100% standard concentration inoculation liquid of the sacculus rhizogenes and the 100% standard concentration inoculation liquid of the sacculus cassava according to a spore ratio of 1:1 to obtain the arbuscular mycorrhizal fungi mixed strain inoculation liquid), and soaking the banana tissue culture seedling in the root for 30 min; and (2) planting the soaked banana tissue culture seedlings in a nutrient cup containing nutrient soil, wherein the nutrient soil is formed by mixing peat soil and coconut chaff according to the volume ratio of 1:2, and uniformly pouring the rest inoculation liquid into the nutrient soil (the specific steps of a control group, treatment 1 and treatment 2 refer to treatment 3, wherein the difference is that the banana tissue culture seedlings are respectively soaked in purified water, 100% standard concentration inoculation liquid of glomus intraradicans and 100% standard concentration inoculation liquid of glomus cassavalis). Under the condition of normal cultivation management, roots, stems and leaves (5 banana plants in each group) of experimental banana tissue culture seedlings growing for 3 months are taken, enzyme deactivation is carried out at 105 ℃, drying is carried out at 80 ℃ to constant weight, and then the dry weight of the seedlings is weighed. The result shows that the single inoculation of the sacculus intraradicis or the sacculus cassava has little influence on the biomass accumulation of the bananas, but the mixed inoculation of the sacculus intraradicis or the sacculus cassava obviously increases the biomass of banana plants. The results are shown in Table 1.
TABLE 1 Banana Biomass from different treatments
Example 2: effect of inoculation of arbuscular mycorrhizal fungi on the number of soil microorganisms of banana seedlings
Experimental design as in example 1, 5 pots of 1 banana seedling per pot for a total of 20 pots were repeated for each group. After 3 months of field planting of banana seedlings, selecting 5 points which are uniformly distributed on the circumference of a position 5-6 cm away from the trunk of the banana seedlings, collecting 8g of soil sample which is about 3-4 cm away from the surface of each point, and carrying out field planting on the banana seedlings5 samples of the experimental group were mixed well and used for soil microbiological assay. The bacteria, fungi, actinomycetes, ammonifying bacteria and nitrogen-fixing bacteria in the soil are respectively prepared by using beef extract peptone culture medium (formula: beef extract 3.0g, peptone 10.0g, NaCl 5.0g, agar 20g, water 1000mL, pH 7.4-7.6) and prepared by dissolving the components in water, stirring, adjusting pH, and sterilizing2PO4 1g、MgSO4·7H20.5g of O, 5g of peptone, 10g of glucose, 20g of agar and 1000mL of water, and the preparation steps are as follows: dissolving the above components in water, stirring, and sterilizing to obtain the final product), and culture medium of Gao's No. I (purchased from Kyoto Kyork Biotechnology Co., Ltd., product number: 028810), peptone agar medium (purchased from Kyork Biotech Co., Ltd., cat #: 025043) and modified arbape nitrogen free agar medium (available from shanghai ruich biotechnology limited, cat #: t2178), and counting the number of bacteria by plate counting method.
Experimental results show that after 3 months of banana seedling culture, statistics on the number of microorganisms shows that inoculation of the arbuscular mycorrhizal fungi mixed strain promotes growth of bacteria, fungi, actinomycetes, nitrogen-fixing bacteria and ammonifying bacteria in soil, and the results are shown in figures 1, 2, 3, 4 and 5 respectively. Therefore, the mixed strain of the arbuscular mycorrhizal fungi is added into the soil, and the growth of the microorganisms is obviously promoted.
Example 3: effect of inoculation of arbuscular mycorrhizal fungi on growth of banana plants
As can be seen from example 2, the inoculation of the arbuscular mycorrhizal fungi mixed strain can increase the number of microorganisms in the soil, and when the banana seedlings grow for three months, the indexes of the plant height, stem circumference (upper, middle and lower), green leaf number, maximum leaf length, maximum leaf width and the like of 5 banana seedlings in each experimental group are measured and averaged, and the results are shown in table 2; the C, N, K, P element content of each of the plant leaves of the experimental groups was measured, and the results are shown in Table 3.
TABLE 2 growth of banana seedlings by different inoculation treatments
TABLE 3 comparison of the leaf element C, N, K, P content of different inoculated plants
The experimental results show that: after the banana seedlings are cultured for three months by the banana tissue culture seedling culture medium inoculated with the arbuscular mycorrhizal fungi mixed strain, the growth of the banana tissue culture seedlings is remarkably promoted, the plant biomass of the banana seedlings is remarkably increased, and the C, N, P, especially the content of K element in plant leaves is improved.
The above is only a preferred embodiment of the present invention, and it should be noted that the above preferred embodiment should not be considered as limiting the present invention, and the protection scope of the present invention should be subject to the scope defined by the claims. It will be apparent to those skilled in the art that various modifications and adaptations can be made without departing from the spirit and scope of the invention, and these modifications and adaptations should be considered within the scope of the invention.
Claims (1)
1. A method for promoting banana seedling growth by inoculating arbuscular mycorrhizal fungi is characterized by comprising the following steps: inoculating an arbuscular mycorrhizal fungi mixed strain to a banana seedling root system, wherein the arbuscular mycorrhizal fungi mixed strain is prepared by mixing Glomus intraradices (Glomus intraradis) and Glomus cassavalis (Glomus manihot) according to a spore ratio of 1: 1;
the banana seedlings are 5 Brazil bananas (Musa acetate. AAA group cv. Cavendish) with consistent growth vigor;
the method is characterized in that the mixed strain of the arbuscular mycorrhizal fungi is inoculated to the root system of the banana seedling, and the specific steps are as follows: cleaning a culture medium in the banana seedlings by using clear water, soaking the banana seedlings in the mixed strain inoculation liquid of the arbuscular mycorrhizal fungi, and soaking the roots for a period of time; planting the soaked banana seedlings in nutrient soil, and uniformly pouring the rest inoculation liquid into the nutrient soil;
the preparation steps of the arbuscular mycorrhizal fungi mixed strain inoculation liquid are as follows: respectively activating intraradicular Glomus (Glomus intraradics) and cassava Glomus (Glomus manihot) in a dark in an incubator at 25 ℃, then respectively preparing a spore suspension with the concentration of 4000 spores/mL by using purified water as a 100% standard concentration inoculation liquid, and mixing the 100% standard concentration inoculation liquid of the intraradicular Glomus and the 100% standard concentration inoculation liquid of the cassava Glomus according to the spore proportion of 1:1 to obtain the mixed strain inoculation liquid of the arbuscular mycorrhizal fungi.
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| AP2010005362A0 (en) * | 2008-01-15 | 2010-08-31 | Univ Catholique Louvain | Method and system for in vitro mass production of arbuscular mycorrhizal fungi |
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