CN111635565A

CN111635565A - Fibroin functional layer for improving mask allergy and preparation method thereof

Info

Publication number: CN111635565A
Application number: CN202010494152.7A
Authority: CN
Inventors: 盛卫琴
Original assignee: Hangzhou Dianzi University
Current assignee: Hangzhou Dianzi University
Priority date: 2020-06-03
Filing date: 2020-06-03
Publication date: 2020-09-08

Abstract

The invention discloses a fibroin functional layer for improving the allergy of a mask and a preparation method thereof, and belongs to the technical field of preparation of sanitary product materials. The preparation method comprises the following steps: 1) preparing a fibroin solution by a conventional degumming method; 2) placing the fibroin solution at a certain temperature for temperature treatment; 3) uniformly mixing the fibroin solution subjected to temperature treatment with a cross-linking agent and alcohol; 4) coating the mixed solution obtained in the step (3) on a corresponding mould, and freeze-drying; 5) and processing the dried fibroin material to obtain a corresponding fibroin functional layer. The fibroin functional layer has good biocompatibility, good moisture absorption, air permeability and degradability, and is an ideal allergy-free mask functional layer material.

Description

Fibroin functional layer for improving mask allergy and preparation method thereof

Technical Field

The invention belongs to the field of preparation of sanitary product materials, and particularly relates to a fibroin functional layer for improving mask allergy and a preparation method thereof.

Background

Coronaviruses are a large family of viruses known to cause the common cold and more serious diseases such as Middle East Respiratory Syndrome (MERS) and Severe Acute Respiratory Syndrome (SARS). The novel coronavirus which is outbreak in 2019 is a novel strain of coronavirus which has never been found in human bodies before, and can cause respiratory symptoms, fever, cough, shortness of breath, dyspnea and even death of infected persons. 12/1/2020 was named 2019-nCoV by the world health organization, and 11/2/2020 was named SARS-CoV-2 by the International Committee for Classification of viruses.

For people, when epidemic situation is prevented, it is a basic requirement that protective tools are worn for self protection and trip, and protective articles such as masks and the like are out of stock once. Meanwhile, the epidemic situation improves the hygiene habits of people, and people can keep the habit of wearing the mask in public places for a long time, so that the development of the high-quality protective mask has wide application prospect and has very important significance for the current healthy life of people all over the world and even the future.

Although various different types of protective masks exist in the market, the mainstream products in the market still have the defects of poor air permeability and moisture permeability and the like. In this epidemic situation, a large amount of medical workers are exposed to the shortcomings of the existing protective products due to the fact that protective equipment is worn for a long time: poor air and moisture permeability and no skin affinity. Therefore, in order to better solve the problems, the fibroin which is rich in source and relatively low in price is adopted as a raw material, the fibroin nanofiber is formed by a self-assembly regulation and control technology, and then the anti-allergic fibroin mask functional layer is prepared.

The silk is natural protein fiber, contains 18 amino acids, wherein leucine can accelerate metabolism of cells, serine and threonine can delay skin aging, and tryptophan and tyrosine can absorb ultraviolet rays, so that various excellent functions of silk fibroin are transferred to the fiber for taking in the way of facing to human bodies, the skin of the human bodies is cared, the human bodies have healthy skin, and the concept of green consumption of the human bodies in the new century is met.

Disclosure of Invention

Aiming at the problems in the prior art, the invention aims to provide a fibroin functional layer for improving the allergy of a mask and a preparation method thereof, and the steps are as follows:

1) degumming silk by a conventional degumming method to prepare a fibroin solution;

2) placing the fibroin solution at a certain temperature for temperature treatment;

3) uniformly mixing the fibroin solution subjected to temperature treatment with a cross-linking agent and alcohol;

4) coating the mixed solution obtained in the step (3) on a corresponding mould, and freeze-drying;

5) and (3) carrying out curing treatment on the dried fibroin material to obtain a corresponding fibroin functional layer.

Preferably, the concentration of the silk protein solution obtained in the step 2) is 0.05% -20%.

Preferably, the treatment temperature of the fibroin solution in the step 2) is 40-90 ℃.

Preferably, the cross-linking agent in step 3) is one or a mixture of alginate and starch.

Preferably, the alcohol in step 3) is one or a mixture of more than two of ethylene glycol, glycerol and tert-butyl alcohol.

Preferably, the mass ratio of the fibroin, the alcohol and the cross-linking agent aqueous solution in the step 3) is 1 (0.2-15): (10-30).

Preferably, the freeze-drying time in the step 4) is 40-80 hours.

Preferably, the curing treatment time of the fibroin in the step 5) is 20-100 hours.

The anti-allergic fibroin porous functional layer prepared by the preparation method has the thickness of 0.5-5 mm, the pore diameter of 150-400 mu m and the porosity of more than 85 percent

The invention designs the mask functional layer material which takes fibroin as the raw material and has good moisture absorption, ventilation and bacteriostasis properties, and the mask functional layer material at least has the following advantages:

1) the fibroin porous material has uniform microstructure and high porosity, and more importantly, the design of the material greatly improves the moisture absorption and air permeability of the mask, so that the aim of improving the allergy of the mask is fulfilled.

2) The process has good controllability of raw materials and preparation, good degradability of the fibroin and meets the requirements of green and environmental protection in China.

Drawings

Fig. 1 is an atomic force microscope image of the fibroin fiber solution prepared in example 1.

Fig. 2 is a microscopic structure view of the fibroin porous functional layer prepared in example 2.

Detailed Description

The invention will be further explained with reference to the drawings.

Example 1:

(1) soaking about 25g of silk in 12L of 0.5% Na according to conventional degumming method₂CO₃The solution is stirred and boiled for 30 minutes, then taken out, washed clean by deionized water and dried at 60 ℃. Then 20g of the dried degummed silk was dissolved in 100ml of 9.3mol/L lithium bromide solution and stirred at 60 ℃ for 4 hours. Then dialyzed in deionized water for 2 days with a dialysis bag (molecular weight cut-off of 3500) to obtain a 5% silk protein aqueous solution.

(2) The fibroin solution is treated at 60 ℃, and then diluted to form a fibroin fiber solution with the concentration of 0.5%, and an atomic force microscope image of the fibroin nanofiber is shown in fig. 1.

(3) 0.5 g of sodium alginate is dissolved in 50mL of water and stirred at normal temperature until the solution is uniform. 10 mu L of ethylene glycol is added dropwise, after stirring and reacting for 1 hour, 0.05g of fibroin is added into the solution, and stirring is continued for 1 hour.

(4) The fibroin mixed solution obtained above was poured on a mold to coat. The material obtained after coating was freeze-dried for 50 h. And after drying, transferring the silk fibroin into an oven to continue drying for 50h to obtain the final silk fibroin porous functional layer material. The functional layer material was subjected to a moisture absorption test, and the results are shown in table 1.

TABLE 1 results of moisture absorption test of chitosan porous functional layer material

Sample name	Initial mass (mg)	Mass after 1 min of water absorption (mg)/Water absorption Capacity	30 min mass (mg) Water absorption rate	1 h mass (mg)/inhale Water multiplying power
					Fibroin porous functional layer material	5	186/37.2	268/53.6	308/61.6

Example 2:

(2) The fibroin solution was treated at 60 ℃ and diluted to a fibroin fiber solution with a concentration of 0.5%.

(3) 0.6 g of starch is dissolved in 50mL of water and stirred at normal temperature until the solution is uniform. Then, 300. mu.L of glycerol was added dropwise thereto, and after stirring and reacting for 1 hour, 0.06g of fibroin was added to the solution, and stirring was continued for 1 hour.

(4) The fibroin mixed solution obtained above was poured on a mold to coat. The material obtained after coating was freeze-dried for 50 h. And after drying, transferring the silk fibroin into an oven to continue drying for 50h to obtain the final silk fibroin porous functional layer material. The microstructure of the functional layer is shown in figure 2. (As can be seen from FIG. 2, the material formed a good porous structure with a thickness of 0.5 to 5 mm, a pore diameter of 150 to 400 μm and a porosity of 85% or more).

Claims

1. A preparation method of a fibroin functional layer for improving the allergy of a mask is disclosed, wherein the fibroin functional layer is prepared from fibroin, and is characterized by comprising the following steps:

2. The silk protein functional layer for improving allergy of a mask and the preparation method thereof according to claim 1, wherein the silk protein solution obtained in the step 1) has a concentration of 0.05-20%.

3. The silk protein functional layer for improving the allergy of the mask and the preparation method thereof according to claim 1, wherein the silk protein solution of step 2) is treated at a temperature of 40-90 ℃.

4. The functional fibroin layer for improving allergy of a mask and the preparation method thereof according to claim 1, wherein the cross-linking agent in step 3) is one or a mixture of alginate and starch.

5. The functional fibroin layer for improving mask allergy according to claim 1, wherein the alcohol in step 3) is one or a mixture of two or more of ethylene glycol, glycerol and tert-butyl alcohol.

6. The fibroin functional layer for improving the allergy of the mask and the preparation method thereof according to claim 1, wherein the mass ratio of the fibroin, the alcohol and the cross-linking agent aqueous solution in the step 3) is 1 (0.2-15): (10-30).

7. The silk protein functional layer for improving allergy of a mask and the preparation method thereof according to claim 1, wherein the freeze-drying time in the step 4) is 40-80 hours.

8. The silk protein functional layer for improving the allergy of the mask and the preparation method thereof according to claim 1, wherein the silk protein curing treatment time in the step 5) is 20-100 hours.

9. The chitosan porous functional layer of the dustproof and bacteriostatic mask prepared according to any of the preparation methods of claims 1 to 8, wherein the thickness of the functional layer material is 0.5 to 5 mm, the pore diameter is 150 to 400 μm, and the porosity is 85% or more.