CN111635279A

CN111635279A - Preparation method of rice wine lees water-soluble fertilizer for inhibiting plant diseases

Info

Publication number: CN111635279A
Application number: CN202010540408.3A
Authority: CN
Inventors: 卢卫红; 周凯; 张友源; 朱云峰; 徐翠翠; 金少瑾
Original assignee: Harbin Institute Of Technology Robot (shandong) Intelligent Equipment Research Institute
Current assignee: Harbin Institute Of Technology Robot (shandong) Intelligent Equipment Research Institute
Priority date: 2020-06-15
Filing date: 2020-06-15
Publication date: 2020-09-08

Abstract

The invention provides a preparation method of a rice wine lees water-soluble fertilizer for inhibiting plant diseases. The preparation method comprises the following steps: s10: adding an enzyme preparation into the vinasse for enzymolysis; s20: adding nutrient elements for fermentation and adding bacteriostatic peptide producing bacteria for fermentation; s30: carrying out solid-liquid separation on the generated fermentation liquor to obtain antibacterial liquid; s40: and treating the antibacterial liquid to obtain the water-soluble fertilizer. By applying the technical scheme of the invention, rich carbon sources, nitrogen sources and other nutrient elements of the rice wine lees are utilized to carry out enzymolysis and microbial fermentation treatment to generate polylysine and lipopeptide antibiotics, and after solid-liquid separation, the water-soluble fertilizer with the bacteriostatic and disease-preventing effects is prepared by treatment. The product has broad-spectrum resistance to pathogenic bacteria, and especially has good effect on fungal diseases. Moreover, the method solves the problem of harmless treatment of the vinasse wastewater, reduces the treatment cost for wineries, and improves the added value to the maximum extent.

Description

Preparation method of rice wine lees water-soluble fertilizer for inhibiting plant diseases

Technical Field

The invention relates to the technical field of fertilizers, in particular to a preparation method of a rice wine lees water-soluble fertilizer for inhibiting plant diseases.

Background

At present, the common domestic agents for preventing and treating plant diseases of crops are mainly agricultural antibiotics, bisultap and inorganic copper preparations. The control effect of agricultural antibiotics and bisultap is increasingly unsatisfactory due to the problems of drug resistance, too long use history and the like, and the inorganic copper preparation has the limitations of phytotoxicity, influence on the proliferation of red spiders, poor mixing property, inconvenient use and the like. Therefore, pure biological, safe and residue-free bactericides are increasingly favored, and polylysine and various lipopeptide antibacterial peptides undoubtedly have the potential and have synergistic effects mutually.

Polylysine is a natural biological metabolite with good bactericidal power and heat stability. The polylysine has a wide antibacterial spectrum, and has obvious inhibiting and killing effects on candida acutangula, rhodotorula farinosa, pichia membranaceus and rhodotorula rosea of saccharomyces, heat-resistant bacillus steatovorus, bacillus coagulans and bacillus subtilis in gram-positive bacteria, and arthrobacter aerogenes, escherichia coli and the like in gram-negative bacteria. But the single use of polylysine has no obvious inhibition on the bacillus subtilis and the aspergillus niger, and the composite treatment of polylysine and acetic acid enhances the inhibition on the bacillus subtilis. The action mechanism of polylysine is mainly characterized by the destruction of the cell membrane structure of microorganisms, causing the interruption of the material, energy and information transmission of cells, which ultimately leads to cell death.

Lipopeptides, also known as fatty acyl peptides, are an important class of antimicrobial peptides, and are microbial secondary metabolites with an amphiphilic structure consisting of a fatty chain and a peptide chain. Has broad-spectrum antifungal effect and certain antibacterial effect, has an antibacterial mechanism different from that of common antibiotics, can form pores to allow cell contents to flow out to die or chelate into one divalent cation so as to inhibit the activity of various enzymes, and is not easy to generate drug resistance. In addition, the peptide substance is easy to decompose in nature, and belongs to an environment-friendly preparation.

However, the cost of raw materials for producing the polylysine and the lipopeptide antibacterial peptide is high at present, so that the final price of the polylysine and the lipopeptide antibacterial peptide water-soluble fertilizer is high, and the economy for preventing and treating plant diseases is poor.

Disclosure of Invention

The invention mainly aims to provide a preparation method of a water-soluble fertilizer containing rice wine lees for inhibiting plant diseases, and aims to solve the problem that the water-soluble fertilizer for inhibiting plant diseases produced in the prior art is poor in economy.

In order to achieve the above object, according to the present invention, there is provided a method for preparing a water-soluble fertilizer of rice wine lees for inhibiting plant diseases, comprising: s10: adding an enzyme preparation into the vinasse for enzymolysis; s20: adding nutrient elements for fermentation and adding bacteriostatic peptide producing bacteria for fermentation; s30: carrying out solid-liquid separation on the generated fermentation liquor to obtain antibacterial liquid; s40: and treating the antibacterial liquid to obtain the water-soluble fertilizer.

In one embodiment, in S30, the fermentation broth is PH adjusted.

In one embodiment, S10 and S20 are performed simultaneously or in steps in sequence.

In one embodiment, in S20, the bacteriostatic peptide-producing bacterium is one or more of actinomycetes, bacillus, and pseudomonas.

In one embodiment, in S10, the enzyme preparation is one or more of cellulase, hemicellulase, xylanase and pectinase.

In one embodiment, in S20, the nutrient elements for fermentation include one or more of molasses, corn flour, glycerol, ammonium sulfate, dipotassium hydrogen phosphate, potassium dihydrogen phosphate, magnesium sulfate, zinc sulfate, ferric sulfate, urea, and yeast extract powder.

In one embodiment, the treating the bacteriostatic solution at S40 includes: and concentrating the antibacterial liquid.

In one embodiment, the treating the bacteriostatic solution at S40 includes: and (5) adding auxiliary materials into the antibacterial liquid for treatment.

In one embodiment, the auxiliary material comprises one or more of acetic acid, glycine, amino acid, humic acid, macroelements, secondary elements and trace elements.

In one embodiment, the treating the bacteriostatic solution at S40 includes: and canning the antibacterial liquid.

By applying the technical scheme of the invention, rich carbon sources, nitrogen sources and other nutrient elements of the rice wine lees are utilized to carry out enzymolysis and microbial fermentation treatment to generate polylysine and lipopeptide antibiotics, and after solid-liquid separation, the water-soluble fertilizer with the bacteriostatic and disease-preventing effects is prepared by treatment. The product has broad-spectrum resistance to pathogenic bacteria, and especially has good effect on fungal diseases. Moreover, the method solves the problem of harmless treatment of the vinasse wastewater, reduces the treatment cost for wineries, and improves the added value to the maximum extent.

In addition to the objects, features and advantages described above, other objects, features and advantages of the present invention are also provided. The present invention will be described in further detail below with reference to the drawings.

Drawings

The accompanying drawings, which are incorporated in and constitute a part of this specification, are included to provide a further understanding of the invention, and are incorporated in and constitute a part of this specification, illustrate exemplary embodiments of the invention and together with the description serve to explain the invention and not to limit the invention. In the drawings:

fig. 1 is a schematic flow chart showing an example of a method for producing a water-soluble fertilizer of rice wine lees for suppressing plant diseases according to the present invention.

Detailed Description

It should be noted that the embodiments and features of the embodiments may be combined with each other without conflict. The present invention will be described in detail below with reference to the embodiments with reference to the attached drawings.

In order to make the technical solutions of the present invention better understood, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

It should be noted that the terms "first," "second," and the like in the description and claims of the present invention and in the drawings described above are used for distinguishing between similar elements and not necessarily for describing a particular sequential or chronological order. It is to be understood that the terms so used are interchangeable under appropriate circumstances for describing embodiments of the invention herein. Furthermore, the terms "comprises," "comprising," and "having," and any variations thereof, are intended to cover a non-exclusive inclusion, such that a process, method, system, article, or apparatus that comprises a list of steps or elements is not necessarily limited to those steps or elements expressly listed, but may include other steps or elements not expressly listed or inherent to such process, method, article, or apparatus.

It is noted that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of example embodiments according to the present application. As used herein, the singular forms "a", "an" and "the" are intended to include the plural forms as well, and it should be understood that when the terms "comprises" and/or "comprising" are used in this specification, they specify the presence of stated features, steps, operations, devices, components, and/or combinations thereof, unless the context clearly indicates otherwise.

Vinasse is a byproduct of the wine making industry, China is a large drinking country and produces thousands of tons of vinasse every year, wherein the rice wine vinasse accounts for a large part, and can only be treated as sewage generally due to high water content of the vinasse, but if the vinasse cannot be treated in time, deterioration is caused, environment pollution is caused, and the operation of the wine making industry is influenced. At present, the main utilization mode of dregs in rice wine lees is feed, and then organic fertilizer is used. In the past, manufacturers feed the ruminant with the vinasse waste water, but with the improvement of environmental protection requirements, some informal feeds are difficult to sell, the treatment of the waste water is more difficult, and a better way is required to solve the problem. And the water content of the rice wine lees is higher, wherein the waste liquid is generally used as waste water for treatment, which not only causes environmental pollution, but also has high treatment cost.

Through the component analysis of the rice wine lees, the rice wine lees is found to contain abundant polysaccharides, cellulose, amino acids, organic acids, macroelements and the like which are suitable to be used as the components of the biological production raw materials. Therefore, the invention takes rice wine lees as a main raw material, develops the water-soluble fertilizer with the antibacterial effect, generates the components of polylysine and lipopeptide antibacterial peptide in the fermentation process, can inhibit the growth of pathogenic bacteria in soil or plants, has the characteristics of safety, environmental protection and no residue, and reduces the cost for producing the water-soluble fertilizer of polylysine and lipopeptide antibacterial peptide.

Fig. 1 shows an example of a method for preparing a water-soluble fertilizer of rice wine lees for inhibiting plant diseases according to the present invention, which comprises the steps of:

s10: adding an enzyme preparation into the vinasse for enzymolysis;

s20: adding nutrient elements for fermentation and adding bacteriostatic peptide producing bacteria for fermentation;

s30: carrying out solid-liquid separation on the generated fermentation liquor to obtain antibacterial liquid;

s40: and treating the antibacterial liquid to obtain the water-soluble fertilizer.

The bacteriostatic function refers to the broad-spectrum inhibition effect on pathogenic bacteria such as bacteria and fungi. The bacteriostatic component generated in the step S20 mainly comprises polylysine and lipopeptide with bacteriostatic function, including one or more of surfactin, iturin and camellin. Polylysine is a homotypic monomer polymer containing 25-30 lysine residues; lipopeptides are composed of peptide fragments consisting of 7 or 10 molecules of amino acids and fatty acid chains which are C13-C18 fatty acid acyl chains. The bacteriostatic component can inhibit one or more of angular leaf spot of cucumber plants, marginal blight, leaf blight, tomato bacterial wilt, canker, leaf spot disease, pepper bacterial wilt, soft rot, leaf spot, cabbage soft rot, black rot, eggplant bacterial wilt, citrus canker, peach punch disease, watermelon plant angular leaf spot, fruit tree root caner disease, cruciferae and cucurbitaceae downy mildew, blight, solanaceae leaf mildew or verticillium wilt.

Preferably, solid-liquid separation is carried out in S30, mainly to remove impurities and ensure that the water content of the final product is less than or equal to 5 percent. The solid-liquid separation can select various separation modes such as centrifugation, membrane separation and the like, wherein the centrifugation speed is 3000-.

As a more preferred embodiment, in S30, the pH of the fermentation broth is adjusted. The pH value of the fermentation liquor is adjusted to 4-8, and the product can be more easily used in soil through pH value adjustment.

In the technical solution of the present embodiment, S10 is performed first, and then S20 is performed. As an alternative embodiment, the above-mentioned S10 and S20 may be performed simultaneously, i.e. simultaneous enzymatic fermentation; or enzymolysis and fermentation can be carried out firstly, namely the enzymolysis and fermentation are carried out step by step.

In S20, the antimicrobial peptide-producing bacteria is one or more of actinomycetes, bacillus, and pseudomonas. Optionally, the actinomycete is one or more of streptococcus albus, northern sporophytes and ergot fungi. Optionally, the bacillus is one or more of bacillus subtilis, bacillus licheniformis, dead bacillus cereus, bacillus amyloliquefaciens and bacillus megaterium. Specifically, in the technical scheme of the invention, the inoculation amount of the antibacterial peptide producing bacteria is 1-15% of the total material weight, wherein the inoculation amount is 1-3 parts of actinomycetes, 1-3 parts of bacillus and 1-3 parts of pseudomonas, the fermentation temperature is 25-40 ℃, the pH is 4.0-7.0, and the fermentation time is 3 d-7 d. Different strains can be fermented simultaneously or step by step, and the content of the finally produced antibacterial peptide should reach 3g/L-30 g/L.

In S10, the enzyme preparation is one or more of cellulase, hemicellulase, xylanase and pectinase. Specifically, in the technical scheme of the invention, 2-3 parts of cellulase, 1-2 parts of hemicellulase, 1-2 parts of xylanase and 1-2 parts of pectinase are adopted. The enzyme dosage is 0.3-3% of the material, the enzymolysis temperature is 30-50 ℃, the PH is 4.0-8.0, and the enzymolysis time is 0-12 h.

In S20, the nutrient elements for fermentation include one or more of molasses, corn flour, glycerin, ammonium sulfate, dipotassium hydrogen phosphate, potassium dihydrogen phosphate, magnesium sulfate, zinc sulfate, ferric sulfate, urea, and yeast extract powder. Specifically, in the technical scheme of the invention, 500 parts of molasses, 500 parts of corn flour, 50 parts of glycerol, 100 parts of ammonium sulfate, 8 parts of dipotassium hydrogen phosphate, 14 parts of monopotassium phosphate, 5 parts of magnesium sulfate, 0.4 part of zinc sulfate, 0.3 part of ferric sulfate, 50 parts of urea and 50 parts of yeast extract powder are adopted.

As an optional implementation manner, in the technical solution of this embodiment, in S40, the processing the bacteriostatic solution includes: carry out concentrated processing to bacteriostat, through concentrated processing, make water-soluble fertilizer more convenient for transport. When the content of the antibacterial peptide is more than 10g/L after liquid phase concentration, the antibacterial effect of the water-soluble fertilizer is better. As another alternative, the bacteriostatic solution may not be concentrated, and the bacteriostatic solution may be directly canned.

As a more preferable embodiment, in S40, the treating the bacteriostatic agent includes: and (5) adding auxiliary materials into the antibacterial liquid for treatment. Optionally, the auxiliary materials comprise one or more of acetic acid, glycine, amino acid, humic acid, macroelements, secondary elements and trace elements. Specifically, in the technical scheme of the invention, 0.5-5 parts of acetic acid, 0.01-10 parts of glycine, 10-30 parts of amino acid, 3-10 parts of humic acid, 20-50 parts of macroelements, 10 parts of secondary elements and 5-50 parts of trace elements are adopted.

The technical scheme of the invention is that the water-soluble fertilizer product contains a large amount of organic acid substances such as humic acid and the like, and can promote absorption of large, medium and trace nutrient elements, growth of crops and yield increase besides the bacteriostatic effect.

The water-soluble fertilizer produced by the technical scheme of the invention not only can reduce the loss of crops caused by diseases and solve the problem of pesticide residue, but also can promote the absorption of nutrient elements and increase the crop yield.

Several specific examples are given below.

Example 1:

taking 1L of rice wine lees, wherein the solid content is 10%, and adding 7% of molasses, 1% of ammonium sulfate, 0.8% of dipotassium hydrogen phosphate, 1.4% of potassium dihydrogen phosphate, 0.5% of magnesium sulfate, 0.04% of zinc sulfate, 0.03% of ferric sulfate and 5% of urea according to the volume percentage of the rice wine lees. Dissolving and stirring well, adjusting pH to 7.0 with alkali solution (ammonia water), sterilizing, adding 1% cellulase and 0.5% amylase, adding Bacillus subtilis seed solution 3%, performing enzymolysis and fermentation at 30 deg.C for 48 hr, adding 7% molasses, and fermenting for 5 days.

Adjusting the pH value to 4-8, and carrying out solid-liquid separation on the fermentation liquor by centrifugation at the rotating speed of 3000 r/min. Through concentration, the concentration of the antibacterial peptide in the liquid phase is controlled at 10g/L, macroelements are supplemented until the content is 20%, the content of humic acid is 3%, and the humic acid water-soluble fertilizer with the antibacterial effect is prepared and meets the NY1106-2010 standard.

Example 2:

taking 1L of rice wine lees with the solid content of 10%, adding 7% of molasses, 1% of ammonium sulfate, 0.8% of dipotassium hydrogen phosphate, 1.4% of potassium dihydrogen phosphate, 0.5% of magnesium sulfate, 0.04% of zinc sulfate, 0.03% of ferric sulfate and 5% of urea into the rice wine lees according to the volume percentage of the rice wine lees, adjusting the pH value to 7.0, adding 1% of cellulase and 0.5% of amylase after sterilization, adding dead bacillus vallismortis according to 3%, carrying out synchronous enzymolysis and fermentation for 48h at the temperature of 30 ℃, adding 7% of molasses, and carrying out co-fermentation for 5 days.

Example 3:

taking 1L of rice wine lees with the solid content of 10%, adding 25% of molasses, 1% of ammonium sulfate, 0.8% of dipotassium phosphate, 1.4% of potassium dihydrogen phosphate, 0.5% of magnesium sulfate, 0.04% of zinc sulfate, 0.03% of ferric sulfate and 5% of urea into the rice wine lees according to the volume percentage of the rice wine lees, adjusting the pH value to 7.0, adding 1% of cellulase and 0.5% of amylase after sterilization, adding streptomyces albus according to 3%, controlling the temperature to be 30 ℃, synchronously performing enzymolysis and fermentation for 48 hours, keeping the concentration of the molasses at 10% -15% and controlling the pH value at 4.0-5.0 in the subsequent fermentation process, and performing co-fermentation for 7 days.

Example 4:

taking 1L of rice wine lees with the solid content of 10%, adding 7% of molasses, 10% of rice corn flour, 1% of ammonium sulfate, 0.8% of dipotassium hydrogen phosphate, 1.4% of monopotassium phosphate, 0.5% of magnesium sulfate, 0.04% of zinc sulfate, 0.03% of ferric sulfate and 5% of urea into the wine lees, adjusting the pH value to 7.0, adding 1% of cellulase and 0.5% of amylase after sterilization, adding 3% of bacillus subtilis and 3% of dead bacillus vallismortis, carrying out enzymolysis and fermentation for 5 days at the temperature of 30 ℃.

Examples effects of the invention

The water-soluble fertilizers in the embodiments 1, 2 and 3 are selected for partial effect display

The display effect is 1:

the fermentation liquors of examples 1, 2 and 3 were subjected to qualitative analysis of bacteriostatic function using a bacteriostatic test, i.e., a bacteriostatic loop method. Fusarium oxysporum cucumber specialization type (cucumber fusarium wilt) and alternaria alternata (early blight) are selected as pathogenic bacteria. The results of the bacteriostatic test are as follows:

TABLE 1 antibacterial effect

The results show that examples 1, 2 and 3 all have bacteriostatic effects against at least one pathogen, with example 1 and example 3 having a better broad spectrum.

And (3) display effect 2:

selecting a sample: the effect comparison adopts a field comparison method, cucumber seedling culture is carried out in advance, and 500 healthy cucumber seedlings are selected. Wherein 100 plants were selected for growth in normal soil for control 1, and the remaining plants were planted in soil treated with cucumber fusarium oxysporum. And (3) planting 100 seedlings in each group of the control group and each treatment group, and applying the water-soluble fertilizer corresponding to each embodiment in an amount of 10 kg/mu in the rest 3 groups except the control group 1 and the control group 2, wherein the normal water-soluble fertilizer of humic acid (meeting the NY1106-2010 standard and containing no antibacterial peptide) is applied in an amount of 10 kg/mu. And observing the growth condition of the plants after transplanting for one month, recording and analyzing the disease condition, and weighing the total weight. The results are statistically as follows:

TABLE 2 field disease resistance test effect

The disease index = Σ (number of disease-causing plants × disease progression)/(number of total investigated plants × highest disease progression) × 100%

The statistics of chart data shows that the yield of vegetables is increased, the leaves are thickened and plants with diseases and insect pests are reduced when the fertilizer is applied, so that the yield of crops can be increased and the stress resistance of the plants with diseases and insect pests can be improved when the water-soluble vinasse fertilizer is applied.

TABLE 3 cucumber blast disease grade division

The relative arrangement of the components and steps, the numerical expressions and numerical values set forth in these embodiments do not limit the scope of the present invention unless specifically stated otherwise. Meanwhile, it should be understood that the sizes of the respective portions shown in the drawings are not drawn in an actual proportional relationship for the convenience of description. Techniques, methods, and apparatus known to those of ordinary skill in the relevant art may not be discussed in detail but are intended to be part of the specification where appropriate. In all examples shown and discussed herein, any particular value should be construed as merely illustrative, and not limiting. Thus, other examples of the exemplary embodiments may have different values. It should be noted that: like reference numbers and letters indicate like items in the following figures, and thus, once an item is defined in one figure, it need not be in a subsequent figure

Which will be discussed further.

Spatially relative terms, such as "above … …," "above … …," "above … …," "above," and the like, may be used herein for ease of description to describe one device or feature's spatial relationship to another device or feature as illustrated in the figures. It will be understood that the spatially relative terms are intended to encompass different orientations of the device in use or operation in addition to the orientation depicted in the figures. For example, if a device in the figures is turned over, devices described as "above" or "on" other devices or configurations would then be oriented "below" or "under" the other devices or configurations. Thus, the exemplary term "above … …" can include both an orientation of "above … …" and "below … …". The device may be otherwise variously oriented (rotated 90 degrees or at other orientations) and the spatially relative descriptors used herein interpreted accordingly.

In the description of the present invention, it is to be understood that the orientation or positional relationship indicated by the orientation words such as "front, rear, upper, lower, left, right", "lateral, vertical, horizontal" and "top, bottom", etc. are usually based on the orientation or positional relationship shown in the drawings, and are only for convenience of description and simplicity of description, and in the case of not making a reverse description, these orientation words do not indicate and imply that the device or element being referred to must have a specific orientation or be constructed and operated in a specific orientation, and therefore, should not be considered as limiting the scope of the present invention; the terms "inner and outer" refer to the inner and outer relative to the profile of the respective component itself.

The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A preparation method of a rice wine lees water-soluble fertilizer for inhibiting plant diseases is characterized by comprising the following steps:

s10: adding an enzyme preparation into the vinasse for enzymolysis;

2. The method for producing a water-soluble fertilizer containing rice wine lees for controlling plant diseases according to claim 1, wherein the pH of the fermentation liquid is adjusted in S30.

3. The method for preparing the water-soluble fertilizer containing rice wine lees for suppressing plant diseases according to claim 1, wherein the step S10 and the step S20 are performed simultaneously or sequentially.

4. The method for preparing the water-soluble fertilizer containing rice wine lees for inhibiting plant diseases according to claim 1, wherein in S20, the bacteriostatic peptide-producing bacteria are one or more of actinomycetes, bacillus and pseudomonas.

5. The method for preparing the water-soluble fertilizer containing rice wine lees for inhibiting plant diseases according to claim 1, wherein in S10, the enzyme preparation is one or more of cellulase, hemicellulase, xylanase and pectinase.

6. The method for preparing the water soluble fertilizer containing rice wine lees for controlling plant diseases according to claim 1, wherein in S20, the nutrient elements for fermentation include one or more of molasses, corn flour, glycerin, ammonium sulfate, dipotassium hydrogen phosphate, potassium dihydrogen phosphate, magnesium sulfate, zinc sulfate, iron sulfate, urea, and yeast extract powder.

7. The method according to claim 1, wherein the step of processing the bacteriostatic agent at S40 comprises: and concentrating the antibacterial liquid.

8. The method according to claim 1, wherein the step of processing the bacteriostatic agent at S40 comprises: and adding auxiliary materials into the antibacterial liquid for treatment.

9. The preparation method of the water-soluble fertilizer containing rice wine lees for inhibiting plant diseases according to claim 8, wherein the auxiliary materials comprise one or more of acetic acid, glycine, amino acids, humic acid, major elements, secondary elements and trace elements.

10. The method according to claim 1, wherein the step of processing the bacteriostatic agent at S40 comprises: and canning the antibacterial liquid.