CN111632005A

CN111632005A - Polypeptide freeze-dried powder mask composition and preparation and use methods thereof

Info

Publication number: CN111632005A
Application number: CN202010519039.XA
Authority: CN
Inventors: 周海军
Original assignee: Coris Cosmetics Shanghai Co ltd
Current assignee: Coris Cosmetics Shanghai Co ltd
Priority date: 2020-06-09
Filing date: 2020-06-09
Publication date: 2020-09-08

Abstract

The invention discloses a polypeptide freeze-dried powder mask composition and a preparation and use method thereof, relating to the technical field of masks, and the technical scheme is that the polypeptide freeze-dried powder mask composition consists of polypeptide freeze-dried powder, freeze-dried solvent, mask liquid and mask cloth; the polypeptide freeze-dried powder is prepared from the following raw materials: mannitol, trehalose, glycine, creatine, alpha-arbutin, tranexamic acid, ascorbic acid, mandelic acid, cortex magnoliae officinalis extract, nonapeptide-1, oligopeptide-5, oligopeptide-1, lecithin and the balance of water; the mask liquid is prepared from the following raw materials: polyglycerol-10, plant soothing agent, dendrobium extract, argania spinosa kernel oil, preservative composition, carbomer, triethanolamine, sugarcane extract, hydroxyethyl cellulose, tremella extract, hydrogenated castor oil, essential oil of rosa damascena, and the balance being made up by using rosa damascena hydrolat. The mask disclosed by the invention has the advantages of high active ingredients, water replenishing and relieving, whitening and repairing.

Description

Polypeptide freeze-dried powder mask composition and preparation and use methods thereof

Technical Field

The invention relates to the technical field of masks, in particular to a polypeptide freeze-dried powder mask composition and a preparation and use method thereof.

Background

With the improvement of the quality of life of people and the pursuit of beauty of people, more and more people pay more attention to the maintenance of facial skin, so that the facial mask is used as a product in daily maintenance of the facial skin, the facial mask covers the face for a short time, the outside air and pollution are temporarily isolated, the temperature of the facial skin is increased, pores of the skin are expanded, the secretion and metabolism of sweat glands are promoted, the oxygen content of the facial skin is increased, products of metabolism of epidermal cells and accumulated grease substances are favorably discharged from the facial skin, the moisture in the facial mask permeates into the horny layer of the epidermis, the skin is softened, and the facial skin is naturally bright and elastic. Therefore, facial masks are widely used because of their advantages of rapid recovery from the skin, stable condition of the face, and convenience in use. However, with the change of the external environment and the change of the internal metabolism of the human body, the facial skin as an outermost tissue of the human body is in different states, so that people can select different facial masks according to different facial conditions. When the facial skin is influenced by the innate genetic factors, polluted by the external environment or inadequately nursed, the problems of red swelling, inflammation, dryness, water shortage, sensitivity, fragility, damaged cuticle, black spots and the like of the facial skin are easily caused, so that the aging progress of the facial skin is accelerated, and the health of the facial skin is influenced.

The patent application document with the prior reference application publication number of CN108434088A discloses a collagen polypeptide skin beautifying mask and a preparation method thereof, wherein the mask comprises 20-40 parts of butanediol, 10-15 parts of glycerol, 1-3 parts of betaine, 0.2-1 part of lactic acid bacteria fermentation product, 0.2-1 part of panthenol, 0.2-1.2 parts of sodium hyaluronate, 0.1-1 part of tocopherol, 2-5 parts of collagen, 0.3-2 parts of dihydroxymethylimidazolyl urea, 0.3-0.8 part of allantoin, 0.1-1.2 parts of carbomer, 0.2-1 part of triethanolamine, 0.5-1 part of methylparaben, 1-5 parts of sclerotium rolfsii gum, 0.1-1 part of glycerophosphoinositide choline salt, 1-1.5 parts of oligopeptide-1, 1.5 parts of oligopeptide-2, 1-1.5 parts of oligopeptide-2 parts of oligopeptide, 0.4-1.5 parts of oligopeptide-5, 0.8-2 parts of EDTA disodium, 0.4-1 part of PEG-40 hydrogenated castor oil, 0.1-0.5 part of essence and 50-60 parts of water. The preparation method of the collagen polypeptide skin beautifying mask comprises the following steps: s1, mixing and dissolving the essence and PEG-40 hydrogenated castor oil for later use; s2, uniformly mixing water, butanediol, glycerol, betaine, panthenol, sodium hyaluronate, tocopherol, collagen, bis (hydroxymethyl) imidazolidinyl urea, allantoin, carbomer, triethanolamine, methylparaben, sclerotium rolfsii, glycerophosphoinositide choline salt, oligopeptide-5, oligopeptide-2, oligopeptide-1 and EDTA disodium, heating to 80-85 ℃, and uniformly stirring; s3, cooling the facial mask mixed solution obtained in the step S2 to 63-68 ℃, adding the lactic acid bacteria fermentation product, and uniformly stirring; s4, filtering the mask mixed solution obtained in the step S4 at low temperature to obtain collagen polypeptide skin beautifying mask paste solution; s5, compounding the collagen polypeptide skin beautifying mask cream liquid obtained in the step S4 to a mask carrier to obtain a collagen polypeptide skin beautifying mask, and attaching pearlescent paper outside the collagen polypeptide skin beautifying mask cream liquid.

Therefore, the collagen polypeptide skin beautifying mask disclosed by the invention contains components for moisturizing, locking water, sterilizing, inhibiting bacteria, softening cutin, removing free radicals, promoting formation of collagen and delaying aging, contains abundant collagen and various active peptide components, provides sufficient nutrient components for facial skin, moisturizes dry facial skin, uninterruptedly supplements moisture for the facial skin, promotes moisture absorption, conditions and repairs the facial skin, maintains a moisture barrier of the facial skin, and enables the facial skin to be elastic, compact, transparent and moist, so that the mask disclosed by the invention has good effects of moisturizing and repairing the face, such as pore fading; repairing black spots caused by aging, insolation and insufficient sleep; and can remove facial wrinkles and make skin smooth, tender and bright.

The above prior art solutions have the following drawbacks: the mask is added with oligopeptide-5, oligopeptide-2 and other materials for conditioning and repairing facial skin, but because oligopeptide-5, oligopeptide-2 and the like are thermosensitive materials, when the mask is prepared at the temperature of 80-85 ℃, the biological activity of the oligopeptide-5, oligopeptide-2 and other materials is easily reduced greatly, so that the effects of moisturizing, soothing, whitening and repairing the sensitive facial skin of the mask are reduced.

Disclosure of Invention

Aiming at the defects in the prior art, the first purpose of the invention is to provide a polypeptide freeze-dried powder mask composition which has the advantages of high active ingredients, water replenishing and relieving, whitening and repairing.

The second purpose of the invention is to provide a preparation method of the polypeptide freeze-dried powder mask composition, which has the advantage of maximally retaining bioactive substances and thermosensitive substances in materials so as to improve the efficacy of the mask.

The third purpose of the invention is to provide a use method of the polypeptide freeze-dried powder mask composition, which has the advantages of simple and convenient use process.

In order to achieve the first object, the invention provides the following technical scheme: a polypeptide lyophilized powder facial mask composition comprises polypeptide lyophilized powder, lyophilized solvent, facial mask solution and membrane cloth;

the weight ratio of the polypeptide freeze-dried powder to the freeze-dried solvent to the mask liquid is 10:1: 5;

the polypeptide freeze-dried powder is prepared from the following raw materials in percentage by weight: 3.561-4.021% of mannitol, 1.56-3.21% of trehalose, 0.238-0.32% of glycine, 0.1-0.25% of creatine, 0.034-0.064% of alpha-arbutin, 0.64-0.9% of tranexamic acid, 0.02-0.06% of ascorbic acid, 0.05-0.15% of mandelic acid, 0.05-0.09% of bark extract of magnolia officinalis, 0.9-1.321% of nonapeptide-1, 0.06-0.1% of oligopeptide-5, 0.36-0.53% of oligopeptide-1, 0.02-0.05% of lecithin and the balance of water to 100%;

the freeze-dried solvent is prepared from the following raw materials in percentage by weight: 8-12% of diglycerol, 0.4-0.6% of preservative composition, 0.4-0.6% of azone and the balance of water to 100%;

the mask liquid is prepared from the following raw materials in percentage by weight: 2-6% of polyglycerol-10, 1.0-2.4% of plant soothing agent, 0.4-0.6% of dendrobium extract, 0.4-0.6% of argania spinosa kernel oil, 0.4-0.6% of preservative composition, 0.1-0.14% of carbomer, 0.1-0.14% of triethanolamine, 0.05-0.15% of sugarcane extract, 0.04-0.06% of hydroxyethyl cellulose, 0.04-0.06% of tremella extract, 0.01-0.014% of hydrogenated castor oil, 0.002-0.004% of Damascus rose essential oil, and the balance of Damascus rose hydrosol is supplemented to 100%.

By adopting the technical scheme, the alpha-arbutin, the nonapeptide-1 and the ascorbic acid in the polypeptide freeze-dried powder can inhibit the activity of tyrosinase in vivo so as to reduce the generation of melanin from the source; the tranexamic acid can prevent the generated melanin from further spreading to peripheral cells, and the tranexamic acid has high stability and safety, so that the mask is not influenced by pregnant women and people with sensitive facial skin; the generated melanin can be further metabolized and shed under the action of mandelic acid in cooperation with the strong antioxidant effects of ascorbic acid and the cortex magnoliae officinalis extract, so that the problem of black spots and color spots of the facial skin is effectively improved, and the whitening effect on the facial skin is comprehensively improved; meanwhile, the ascorbic acid, the magnolia bark extract and other materials also have the anti-inflammatory and anti-allergy effects on the facial skin, so that the sensitive damaged facial skin can be effectively repaired and conditioned. Oligopeptide-5 and oligopeptide-1 increase the thickness of stratum corneum by specifically stimulating the metabolism of epithelial cells and promoting the growth of cells, thereby enhancing the activity of collagen and elastin, effectively repairing and increasing the elasticity of facial skin, delaying the aging process of facial skin and prolonging the young state of facial skin. In addition, nonapeptide-1, oligopeptide-5 and oligopeptide-1 can be quickly and effectively absorbed by the facial skin due to the small molecular weight, so that the amino acids contained in the facial skin can be replaced to participate in the saccharification reaction, the amino acids contained in the facial skin are prevented from being saccharified, and the anti-saccharification effect is further achieved. When the oligopeptide-5, oligopeptide-1 and other materials are stored in a freeze-dried powder state, the biological activity of the oligopeptide-5, oligopeptide-1 and other materials can be improved, and the storage environment of the high oligopeptide-5, oligopeptide-1 and other materials is optimized, so that the addition of preservatives in the polypeptide freeze-dried powder is reduced, and the safety of the facial skin is improved. The freeze-dried solvent can quickly recover the biological activity of each material in the polypeptide freeze-dried powder, and the azone in the freeze-dried powder can be used as a skin penetration enhancer with excellent penetration effect, so that each material in the polypeptide freeze-dried powder can be quickly absorbed by the skin of the face, and the whitening and repairing effects of the polypeptide freeze-dried powder on the skin of the face are further improved.

The Damascus rose hydrosol is used as a saturated distillation stock solution of a plant to replace water as a solvent in the mask solution, so that the Damascus rose hydrosol can quickly replenish water for the facial skin, and various active ingredients and 0.3-0.5% of essential oil water-soluble ingredients in the Damascus rose are reserved, so that the Damascus rose hydrosol has good effects of replenishing water, relieving allergy and resisting bacteria; in addition, the Damascus rose hydrosol also contains tannin, flavonoid and other substances which are not contained in the essential oil, so that the Damascus rose hydrosol has good antioxidation effect. When the dendrobium extract, the tremella extract and the sugarcane extract are combined, the dendrobium extract, the tremella extract and the sugarcane extract have remarkable effects of moisturizing, soothing and diminishing inflammation on facial skin, so that the dendrobium extract, the tremella extract and the sugarcane extract can be used for conditioning facial skin in time, and also have the effects of whitening, fading spots and resisting oxidation after being used for a long time. The whole mask liquid is not added with water, so that the growth and the propagation of most microorganisms in the mask liquid can be inhibited, and meanwhile, the antibacterial effect of the rosa damascena hydrosol is combined, so that the antibacterial effect on the microorganisms in the mask liquid is further improved, and the addition amount of the preservative is reduced. The addition of the essential oil of the rosa damascena improves the effects of water locking, moisture preservation and soothing and repairing of the facial mask liquid, and substances such as terpenes and the like in the essential oil of the rosa damascena can provide aromatic odor for the facial mask liquid so as to relieve the tension of the facial skin. In addition, the essential oil of the rosa damascena can bring smooth skin feeling to the facial mask liquid by matching the oil phase texture of the essential oil of the rosa damascena with the hydrogenated castor oil and the argania spinosa kernel oil. The plant soothing agent added into the facial mask liquid further enhances the soothing and repairing effects of the facial mask liquid and improves the safety of facial skin.

Therefore, the biological activity of each material in the freeze-dried powder is improved by freeze-drying each material in the polypeptide freeze-dried powder, and the storage condition of each material in the freeze-dried powder is optimized, so that the addition of a preservative in the polypeptide freeze-dried powder is reduced, and the safety to the facial skin is improved. After the polypeptide freeze-dried powder is dissolved by the solvent, the biological activity of each material in the polypeptide freeze-dried powder can be quickly recovered, so that each material in the polypeptide freeze-dried powder can be quickly and effectively absorbed by facial skin, and the effects of repairing, resisting oxidation and resisting saccharification of the facial skin by the polypeptide freeze-dried powder are further improved; the Damascus rose hydrosol is used for replacing water as a solvent in the mask liquid, the usage of preservatives in the mask liquid is reduced while the facial skin is rapidly moisturized, so that the safety of the facial skin is further improved, meanwhile, each plant extract added in the mask liquid can effectively condition the facial skin, and after the plant extract is added into the polypeptide freeze-dried powder, each material has a synergistic effect, the facial skin can be effectively and slowly repaired while the moisturizing effect of the mask is exerted, the aging progress of the facial skin is delayed, and the young state of the facial skin is prolonged. Trehalose and mannitol homoenergetic form one deck protection film on the material surface of required freeze-drying, not only can carry out effectual protection but also can provide good freeze-drying skeleton for the material in the freeze-drying process to the freeze-drying process of material, play the effect of molding to the material, add the glycine and improved the anti-sensitivity to facial skin, protect severe sensitive facial skin. In addition, glycine, trehalose and mannitol all have good moisturizing and skin moistening effects, so that the moisturizing and skin moistening effects of the whole polypeptide freeze-dried powder are enhanced.

Further: the plant soothing agent is prepared from the following raw materials: stevioside, scutellaria root extract, flavescent sophora root extract and licorice root extract.

By adopting the technical scheme, the radix scutellariae extract, the radix sophorae flavescentis extract and the radix glycyrrhizae inflatae extract have extremely strong anti-inflammatory and anti-allergy effects, can have a good relieving effect on facial skin when being matched with stevioside, and can reduce the release of allergic media, so that the allergic symptoms of the facial skin are effectively relieved, and the resistance and the repairing effect of the facial skin are further improved. The flavonoid substances in the scutellaria baicalensis root extract have good oxidation resistance and capability of repairing damaged facial skin, so that the aging progress of the facial skin can be further delayed.

Further: the dendrobium extract is a dendrobium officinale extract.

By adopting the technical scheme, the polysaccharide and flavonoid substances contained in the dendrobium officinale can improve the moisture retention and oxidation resistance of the whole facial mask liquid. Meanwhile, the stilbene substances in the dendrobium officinale can inhibit the activity of hyaluronidase to improve the moisturizing and anti-inflammatory effects of materials such as the rosa damascena hydrosol, the tremella extract and the sugarcane extract in facial mask liquid, and can also inhibit the release of histamine in facial skin and promote the release of an allergic medium in the sophora flavescens root extract, so that the skin allergy symptom is effectively relieved.

Further: the preservative composition is prepared from the following components in percentage by weight of 1: 10:(10-20): (10-20): (20-40) ethylhexylglycerin: p-hydroxyacetophenone: caprylyl hydroximic acid, glyceryl caprylate and 1, 3-propylene glycol.

By adopting the technical scheme, the glyceryl caprylate has gram-resistant activity, the effect of an anti-acne product can be improved, the glyceryl caprylate can synergically sterilize, after the glyceryl caprylate and the antifungal component are compounded, all related microorganisms are quickly and effectively destroyed, and a short lipophilic carbon chain of the glyceryl caprylate permeates into and is adsorbed on a lipid part of human skin, so that a hydrophilic group of the glyceryl caprylate can better keep moisture in a horny layer, and an oily component can be conveyed and kept on the surface layer of the skin; octanoyl hydroximic acid P-Fe²⁺And Fe³⁺The high-efficiency selective chelation effect is achieved, and the growth of mould is limited in the environment with limited iron ions; iron is a key element for microbial growth, and microbes release chelators to capture Fe from the environment³⁺And converting it to Fe²⁺(ii) a Octanoyl hydroximic acid chelated Fe³⁺The stability constant of the compound is high, so that the compound can prevent mould from obtaining iron element and can effectively inhibit the mould; 1, 3-propanediol is used as an excellent humectant, can destroy microbial cell membranes and then form vacuoles, has broad-spectrum antibacterial activity and can resist escherichia coli, pseudomonas aeruginosa, staphylococcus aureus, candida albicans and aspergillus niger; the p-hydroxyacetophenone has oxidation resistance and irritation resistance, can influence the proliferation of microorganisms by interfering the activity of coenzyme for synthesizing cell membranes, is also a good preservative promoter and can promote the preservative efficacy of various preservatives; the ethylhexyl glycerin can enhance the bacteriostatic effect of alcohols and glycols in cosmetics, thereby enhancing the bacteriostatic and bactericidal effects of the whole preservative composition, and the preservative composition has no stimulation to human bodies, no toxic or side effect and is safe to use.

In order to achieve the second object, the invention provides the following technical scheme: a preparation method of a polypeptide freeze-dried powder mask composition comprises the following steps:

s1: weighing materials: weighing the materials in proportion;

s2: preparing polypeptide freeze-dried powder:

s21: heating water to 80-90 deg.C;

s22: cooling the water obtained in S21 to 40-50 deg.C, adding mannitol, trehalose, glycine, creatine, alpha-arbutin, tranexamic acid, ascorbic acid, mandelic acid, cortex Magnolia officinalis extract, nonapeptide-1, oligopeptide-5, and oligopeptide-1; evenly stirring and mixing lecithin to obtain colorless and transparent polypeptide freeze-dried powder solution;

s23: cooling the polypeptide freeze-dried powder solution obtained in the S22 to 38 ℃, adjusting the pH value to 5.0-7.0, and performing report inspection;

s24: performing vacuum freeze-drying on the qualified polypeptide freeze-dried powder solution obtained in the step S23 to obtain white polypeptide freeze-dried powder;

s3: preparation of freeze-dried solvent:

s31: heating water to 80-90 deg.C;

s32: cooling the water obtained in S31 to 40-50 ℃, adding the preservative composition and azone, stirring and mixing uniformly to obtain a colorless and transparent freeze-dried solvent solution;

s33: cooling the freeze-dried solvent solution obtained in the step S32 to 38 ℃, adjusting the pH value to 4.5-6.0, and performing report inspection;

s4: preparing mask liquid:

s41: premixing hydrogenated castor oil and Damascus rose essential oil to obtain a premix;

s42: heating carbomer, hydroxyethyl cellulose, polyglycerol-10 and Rosa damascena hydrosol to 80-90 deg.C to obtain hot solution of facial mask liquid;

s43: emulsifying the hot solution of the facial mask solution obtained in the step S42 to form a uniform facial mask solution;

s44: cooling the emulsified facial mask solution in the step S43 to 45-55 ℃, adding triethanolamine, and then continuously cooling to 40-50 ℃ to obtain a cooled facial mask solution;

s45: adding the cooled mask liquid solution in the S44 into the dendrobium extract, the plant soothing agent, the tremella extract, the preservative composition, the sugarcane extract, the Aralia mandshurica kernel oil and the premix prepared in the S41, uniformly stirring, adjusting the pH value to 5.4-6.4 to obtain light yellow transparent viscous mask liquid, and then performing inspection reporting;

s5: assembling:

s51: bagging a piece of membrane cloth, adding the facial mask liquid prepared in S45, and packaging to obtain a packaging bag 1;

s52: bagging the polypeptide freeze-dried powder prepared in the step S24, packaging to obtain a packaging bag 2, bagging the freeze-dried solvent prepared in the step S33, and packaging to obtain a packaging bag 3;

s53: assembling the packaging bag 1 in the S51, the packaging bag 2 and the packaging bag 3 in the S52 to form a polypeptide freeze-dried powder mask composition;

s54: and (5) sterilizing and reporting the polypeptide freeze-dried powder mask composition in the step S53, and leaving the factory after the report is qualified.

By adopting the technical scheme, the oligopeptide-5, oligopeptide-1 and other materials are stored in a freeze-dried powder state by adopting a freeze-drying technology, so that the bioactive substances and the heat-sensitive substances in the oligopeptide-5, oligopeptide-1 and other materials are reserved to the maximum extent, and the storage conditions of the oligopeptide-5, oligopeptide-1 and other materials are optimized, thereby reducing the addition of preservatives in the polypeptide freeze-dried powder and improving the safety of facial skin.

Further: vacuum freeze-drying conditions in said S24: the freezing temperature is-35 deg.C to-45 deg.C, and the freezing time is 20-30 min; the sublimation temperature is-8-0 deg.C, the vacuum degree is 20-30Pa, and the sublimation time is 8-16 h; the water content of the freeze-dried powder after vacuum freeze-drying is less than or equal to 3 percent.

By adopting the technical scheme, the activity of the effective components in the materials can be retained to the maximum extent through the conditions, and the preservation time of the freeze-dried powder can be prolonged under the condition that no preservative is added.

Further: the viscosity range of the facial mask solution prepared in the S45 is 1000-1500mPa & S.

By adopting the technical scheme, when the viscosity range of the facial mask liquid is 1000-1500mPa & s, the loss of the facial mask liquid from the facial skin can be reduced, and the obstruction of the facial mask liquid in absorption of the facial mask liquid by the facial skin can be reduced.

Further: in order to achieve the third object, the invention provides the following technical solutions: a use method of a polypeptide freeze-dried powder mask composition comprises the following steps: opening the packaging bag 1, the packaging bag 2 and the packaging bag 3, extruding the freeze-dried solvent in the packaging bag 3 into the polypeptide freeze-dried powder in the packaging bag 2, mixing uniformly, extruding into the mask liquid with the membrane cloth in the packaging bag 1, standing for 2-3min, and taking out the membrane cloth for use.

By adopting the technical scheme, the active substances in the polypeptide freeze-dried powder can be quickly dissolved in the freeze-dried solvent and then mixed with the mask liquid, so that the active substances in the mask liquid and the active substances in the polypeptide freeze-dried powder are fully mixed and soaked in the membrane cloth to exert the effect, and the use process is simple and convenient.

In conclusion, the invention has the following beneficial effects:

firstly, because the freeze-drying preservation of all the materials in the polypeptide freeze-dried powder is adopted, the biological activity of all the materials in the polypeptide freeze-dried powder is improved, and the preservation conditions of all the materials in the polypeptide freeze-dried powder are optimized, so that the addition of a preservative in the polypeptide freeze-dried powder is reduced, and the safety of the facial skin is improved. After the polypeptide freeze-dried powder is dissolved by the solvent, the biological activity of each material in the polypeptide freeze-dried powder can be quickly recovered, so that each material in the polypeptide freeze-dried powder can be quickly and effectively absorbed by facial skin, and the effects of repairing, resisting oxidation and resisting saccharification of the facial skin by the polypeptide freeze-dried powder are further improved; the Damascus rose hydrosol is used for replacing water as a solvent in the mask liquid, so that the use of preservatives in the mask liquid is reduced while the facial skin is rapidly moisturized, the safety of the facial skin is further improved, meanwhile, each plant extract added in the mask liquid can effectively condition the facial skin, and after the extract is added into the polypeptide freeze-dried powder, each material has a synergistic effect, the moisturizing effect of the mask is exerted, the effective relieving and repairing of the facial skin can be realized, the aging progress of the facial skin is delayed, and the young state of the facial skin is prolonged;

secondly, the radix scutellariae extract, the radix sophorae flavescentis extract and the radix glycyrrhizae inflatae extract adopted by the invention have extremely strong anti-inflammatory and anti-allergy effects, and can have a good relieving effect on skin when being matched with stevioside, and the radix sophorae flavescentis extract can reduce the release of allergic media, so that the skin allergy symptoms are effectively relieved, and the resistance and the repair effect of the facial skin are further improved. The flavonoid substances rich in the scutellaria baicalensis root extract can have good capacities of resisting oxidation and repairing damaged facial skin, so that the aging progress of the facial skin can be further delayed;

thirdly, the preparation method adopts the freeze-drying technology to preserve the oligopeptide-5, the oligopeptide-1 and other materials in a scheduled manner, so that the bioactive substances and the heat-sensitive substances in the oligopeptide-5, the oligopeptide-1 and other materials are reserved to the maximum extent, and the preservation environment of the oligopeptide-5, the oligopeptide-1 and other materials is optimized, thereby reducing the addition of preservatives in the polypeptide freeze-dried powder and improving the safety of facial skin.

Detailed Description

The present invention will be described in further detail below.

Preparation of plant soothing agent

Preparation example 1 of plant soothing agent:

s1: weighing materials: according to the following steps of 20: 5: 5: 4, weighing the stevioside, the scutellaria root extract, the sophora flavescens root extract and the glycyrrhiza inflata root extract according to the proportion;

s2: mixing: and (4) uniformly mixing the materials in the S1 to obtain the plant soothing agent.

Preparation of preservative composition materials used in the respective preparation examples of the preservative composition: the ETHYLHEXYLGLYCERIN is ETHYLHEXYLGLYCERIN; p-HYDROXYACETOPHENONE is hydroxyyacetophenone; the caprylhydroxamic acid is caprilhydroxacicacid; caprylic acid glyceride GLYCERYLCAPRYLATE; the 1, 3-PROPANEDIOL is PROPANEDIOL.

Preparation example 1 of preservative composition:

s1: weighing materials: respectively weighing ethylhexyl glycerol, p-hydroxyacetophenone, caprylyl hydroxamic acid, glyceryl caprylate and 1, 3-propylene glycol according to the proportion of 3:30:10:10: 40;

s2: mixing: and (4) uniformly mixing the materials in the S1 to obtain the preservative composition.

Preparation example 2 of preservative composition:

s1: weighing materials: respectively weighing ethylhexyl glycerol, p-hydroxyacetophenone, caprylyl hydroxamic acid, glyceryl caprylate and 1, 3-propylene glycol according to the proportion of 3:30:15:15: 30;

Preparation example 3 of preservative composition:

s1: weighing materials: respectively weighing ethylhexyl glycerol, p-hydroxyacetophenone, caprylyl hydroxamic acid, glyceryl caprylate and 1, 3-propylene glycol according to the ratio of 3:30:20:20: 20;

Examples

Materials used in the examples: GLYCINE is GLYCINE; TREHALOSE is TREHALOSE; MANNITOL is manitol; CREATINE is CREATINE; OLIGOPEPTIDE-5 is OLIGOPEPTIDE-5; OLIGOPEPTIDE-1 is OLIGOPEPTIDE-1; tranexamic acid is TRANEXAMICACID; ascorbic acid is ascorbyl; mandelic acid MANDELICACID; cortex Magnolia officinalis extract is MAGNOLIIAOFFICINALISARBARKESTRACT; LECITHIN is LECITHIN; azone is; hydrogenated castor oil is hydrogoenatedcasotoil; damascus rose essential oil was supplied by Paris corporation; CARBOMER is CARBOMER; the hydroxyethyl cellulose is HYDROXYETHYLCELLULOSE; the polyglycerol-10 is POLYGLYCERIN-10; the Damascus rose truffle was provided by Weifang Naao cosmetics Co., Ltd; the TRIETHANOLAMINE is Triethanolamine; the herba Dendrobii extract is Dendrobii nobilextract; the herba Dendrobii extract is Dendrobii candindump extract; the Tremella extract is Tremellafuciformis extract; the sugar cane EXTRACT is Saccharum officinarum (sugar cane) EXTRACT; the Aralia elata seed oil is Arganiasphinoxakerneliol; PHENOXYETHANOL is phenoxythanol; methyl paraben (METHYLPARABEN) is Methylparaben; the ALPHA-ARBUTIN is ALPHA-ARBUTIN; NONAPEPTIDE-1 is NONAPEPTIDE-1; the STEVIOSIDE is stevia; the Scutellariae radix extract is Scutellaria baicalensis root extract; the radix Sophorae Flavescentis extract is SOPHORANGUSTIFOLIAROOXTRACT; the extract of the root of Glycyrrhiza inflata is Glycyrrhiza inflata extract; the rest are all commercially available cosmetic raw materials.

Example 1:

s1: weighing materials: weighing the materials in proportion;

s2: preparing polypeptide freeze-dried powder:

s21: heating water to 85 deg.C;

s22: cooling the water obtained in S21 to 45 ℃, adding 3.809kg of mannitol, 1.904kg of trehalose, 0.238kg of glycine, 0.2kg of creatine, 0.05kg of alpha-arbutin, 0.8kg of tranexamic acid, 0.02kg of ascorbic acid, 0.05kg of mandelic acid, 0.05kg of cortex magnoliae officinalis extract, 1kg of nonapeptide-1, 0.1kg of oligopeptide-5, 0.5kg of oligopeptide-1 and 0.05kg of lecithin, and uniformly stirring and mixing to obtain a colorless and transparent polypeptide freeze-dried powder solution;

s23: naturally cooling the polypeptide freeze-dried powder solution obtained in the step S22 to 38 ℃, adjusting the pH value to 6, and performing report inspection;

s24: performing vacuum freeze-drying on the qualified mixed solution obtained in the step S23, wherein the specific process comprises the steps of firstly freezing for 20min at-45 ℃, and then sublimating for 12h under the conditions that the temperature is-6 ℃ and the vacuum degree is 30Pa until white polypeptide freeze-dried powder with the water content less than or equal to 3% is obtained;

s3: preparation of freeze-dried solvent:

s31: heating water to 85 deg.C;

s32: cooling the water obtained in the step S31 to 45 ℃, adding 0.4kg of preservative composition and 0.4kg of azone, and uniformly stirring and mixing to obtain a colorless and transparent freeze-dried solvent solution;

s33: cooling the freeze-dried solvent solution obtained in the step S32 to 38 ℃, adjusting the pH value to 6, and performing report inspection;

s4: preparing mask liquid:

s41: premixing 0.01kg of hydrogenated castor oil and 0.002kg of essential oil of Damascus rose to obtain a premix;

s42: heating 0.1kg of carbomer, 0.04kg of hydroxyethyl cellulose, 2kg of polyglycerol-10 and 86kg of Damascus rose hydrosol to 85 ℃ to obtain a hot solution of facial mask liquid;

s44: cooling the emulsified facial mask solution in the step S43 to 50 ℃, adding 0.1kg of triethanolamine, and then continuously cooling to 45 ℃ to obtain a cooled facial mask solution;

s45: adding 1kg of plant soothing agent, 0.4kg of dendrobium extract, 0.04kg of tremella extract, 0.4kg of preservative composition, 0.05kg of sugarcane extract, 0.4kg of argania spinosa kernel oil and the pre-mask mixed solution prepared in the step S41 into the cooled mask solution in the step S44, uniformly stirring, adjusting the pH value to 6 to obtain light yellow transparent mask solution with the viscosity of 1000mPa & S, and performing inspection report;

s5: assembling:

s51: bagging a piece of membrane cloth, adding 25g of the facial mask liquid prepared in S45, and packaging to obtain a packaging bag 1;

s52: 50g of the polypeptide freeze-dried powder prepared in the step S24 is bagged and packaged to obtain a packaging bag 2, and 5g of the freeze-dried solvent prepared in the step S33 is bagged and packaged to obtain a packaging bag 3;

The preservative composition used in this example was phenoxyethanol.

Example 2:

s1: weighing materials: weighing the materials in proportion;

s2: preparing polypeptide freeze-dried powder:

s21: heating water to 85 deg.C;

s22: cooling the water obtained in S21 to 45 ℃, adding the water obtained in S21, cooling to 45 ℃, adding 4kg of mannitol, 2kg of trehalose, 0.3kg of glycine, 0.1kg of creatine, 0.034kg of alpha-arbutin, 0.64kg of tranexamic acid, 0.3kg of ascorbic acid, 0.075kg of mandelic acid, 0.06kg of cortex magnoliae officinalis bark extract, 1.2kg of nonapeptide-1, 0.08kg of oligopeptide-5, 0.36kg of oligopeptide-1 and 0.045kg of lecithin, stirring and mixing uniformly to obtain a colorless and transparent polypeptide freeze-dried powder solution;

s23: naturally cooling the polypeptide freeze-dried powder solution obtained in the step S22 to 38 ℃, adjusting the pH value to 6.5, and performing report inspection;

s3: preparation of freeze-dried solvent:

s31: heating water to 85 deg.C;

s32: cooling the water obtained in the step S31 to 45 ℃, adding 0.5kg of preservative composition and 0.5kg of azone, and uniformly stirring and mixing to obtain a colorless and transparent freeze-dried solvent solution;

s33: cooling the freeze-dried solvent solution obtained in the step S32 to 38 ℃, adjusting the pH value to 5.5, and performing report inspection;

s4: preparing mask liquid:

s41: premixing 0.012kg of hydrogenated castor oil and 0.003kg of Damascus rose essential oil to obtain a premix;

s42: heating carbomer 0.12kg, hydroxyethyl cellulose 0.05kg, polyglycerol-10 4kg and Rosa damascena hydrosol 90kg to 85 deg.C to obtain hot solution of facial mask liquid;

s44: cooling the emulsified facial mask solution in the step S43 to 50 ℃, adding 0.12kg of triethanolamine, and then continuously cooling to 45 ℃ to obtain a cooled facial mask solution;

s45: adding 1.7kg of plant soothing agent, 0.5kg of dendrobium extract, 0.05kg of tremella extract, 0.5kg of preservative composition, 0.1kg of sugarcane extract, 0.5kg of argania spinosa kernel oil and the pre-mask mixed solution prepared in the step S41 into the cooled mask solution in the step S44, uniformly stirring, adjusting the pH value to 6 to obtain a light yellow transparent mask solution with the viscosity of 1000mPa & S, and then performing inspection report;

s5: assembling:

The preservative composition used in this example was phenoxyethanol.

Example 3:

s1: weighing materials: weighing the materials in proportion;

s2: preparing polypeptide freeze-dried powder:

s21: heating water to 85 deg.C;

s22: cooling the water obtained in S21 to 45 ℃, adding the water obtained in S21 to 45 ℃, adding 3.751kg of mannitol, 1.862kg of trehalose, 0.258kg of glycine, 0.158kg of creatine, 0.064kg of alpha-arbutin, 0.754kg of tranexamic acid, 0.04kg of ascorbic acid, 0.1kg of mandelic acid, 0.07kg of cortex magnoliae officinalis bark extract, 1.321kg of nonapeptide-1, 0.094kg of oligopeptide-5, 0.45kg of oligopeptide-1 and 0.03kg of lecithin, and uniformly stirring and mixing to obtain a colorless and transparent polypeptide freeze-dried powder solution;

s3: preparation of freeze-dried solvent:

s31: heating water to 85 deg.C;

s32: cooling the water obtained in the step S31 to 45 ℃, adding 0.6kg of preservative composition and 0.6kg of azone, and uniformly stirring and mixing to obtain a colorless and transparent freeze-dried solvent solution;

s4: preparing mask liquid:

s41: premixing 0.014kg of hydrogenated castor oil and 0.004kg of essential oil of rosa damascena to obtain a premix;

s42: heating 0.14kg of carbomer, 0.06kg of hydroxyethyl cellulose, 6kg of polyglycerol-10 and 92kg of Damascus rose hydrosol to 85 ℃ to obtain a hot solution of facial mask liquid;

s44: cooling the emulsified facial mask solution in the step S43 to 50 ℃, adding 0.14kg of triethanolamine, and then continuously cooling to 45 ℃ to obtain a cooled facial mask solution;

s45: adding 2.4kg of plant soothing agent, 0.6kg of dendrobium extract, 0.06kg of tremella extract, 0.6kg of preservative composition, 0.15kg of sugarcane extract, 0.6kg of argania spinosa kernel oil and the pre-mask mixed solution prepared in the step S41 into the cooled mask solution in the step S44, uniformly stirring, adjusting the pH value to 6 to obtain a light yellow transparent mask solution with the viscosity of 1000mPa & S, and then performing inspection report;

s5: assembling:

The preservative composition used in this example was phenoxyethanol.

Example 4:

the present embodiment is different from embodiment 1 in that, in the present embodiment, step S22: cooling the water obtained in S21 to 45 ℃, adding the water obtained in S21 to 45 ℃, adding 4.021kg of mannitol, 1.56kg of trehalose, 0.32kg of glycine, 0.18kg of creatine, 0.063kg of alpha-arbutin, 0.65kg of tranexamic acid, 0.05kg of ascorbic acid, 0.125kg of mandelic acid, 0.08kg of cortex magnoliae officinalis bark extract, 1.21kg of nonapeptide-1, 0.06kg of oligopeptide-5, 0.53kg of oligopeptide-1 and 0.035kg of lecithin, and uniformly stirring and mixing to obtain the colorless and transparent polypeptide freeze-dried powder solution.

The preservative composition used in this example was phenoxyethanol.

Example 5:

the present embodiment is different from embodiment 1 in that, in the present embodiment, step S22: cooling the water obtained in S21 to 45 ℃, adding 3.561kg of mannitol, 3.21kg of trehalose, 0.31kg of glycine, 0.25kg of creatine, 0.055kg of alpha-arbutin, 0.9kg of tranexamic acid, 0.06kg of ascorbic acid, 0.15kg of mandelic acid, 0.09kg of cortex magnoliae officinalis bark extract, 0.9kg of nonapeptide-1, 0.2kg of oligopeptide-5, 0.47kg of oligopeptide-1 and 0.025kg of lecithin into the water obtained in S21, cooling the water to 45 ℃, adding the water, stirring and mixing uniformly to obtain a colorless and transparent polypeptide freeze-dried powder solution.

The preservative composition used in this example was phenoxyethanol.

Example 6:

this example differs from example 1 in that the plant soothing extract in step S45 in this example was prepared from rebaudioside, scutellaria baicalensis root extract, sophora flavescens root extract, glycyrrhiza inflata root extract according to a ratio of 20: 5: 5: 4 in proportion.

The preservative composition used in this example was phenoxyethanol.

Example 7:

this example differs from example 1 in that the preservative composition in step S32 in this example was prepared from preservative composition prepared in preparation example 1.

Example 8:

this example differs from example 1 in that the preservative composition in step S32 in this example was prepared from preservative composition preparation 2.

Example 9:

this example differs from example 1 in that the preservative composition in step S32 in this example was prepared from preservative composition preparation 3.

Example 10:

this example differs from example 1 in that the preservative composition in step S45 in this example was prepared from preservative composition prepared in preparation example 1.

Example 11:

this example differs from example 1 in that the preservative composition in step S45 in this example was prepared from preservative composition preparation 2.

Example 12:

this example differs from example 1 in that the preservative composition in step S45 in this example was prepared from preservative composition preparation 3.

Example 13:

the difference between this embodiment and embodiment 1 is that the dendrobium extract in step S45 is a dendrobium officinale extract.

Example 14:

this example differs from example 1 in that the preservative composition in step S32 was prepared from preservative composition prepared in preparative example 1; the preservative composition in step S45 is prepared from preparation example 1 of a preservative composition; in the step S45, the dendrobium extract is a dendrobium officinale extract.

Comparative example 1:

the present comparative example is different from example 1 in that alpha-arbutin and nonapeptide-1 are not added in step S22 of the present comparative example.

Comparative example 2:

the present comparative example is different from example 1 in that ascorbic acid, mandelic acid, and magnolia bark extract are not added in step S22 of the present comparative example.

Comparative example 3:

this comparative example is different from example 1 in that no oligopeptide-5 and no oligopeptide-1 are added in step S22 in this comparative example.

Comparative example 4:

this comparative example differs from example 1 in that step S22: cooling the water obtained in S21 to 45 ℃, adding 0.2kg of creatine, 0.05kg of alpha-arbutin, 0.8kg of tranexamic acid, 0.02kg of ascorbic acid, 0.05kg of mandelic acid, 0.05kg of cortex magnoliae officinalis bark extract, 1kg of nonapeptide-1, 0.1kg of oligopeptide-5, 0.5kg of oligopeptide-1 and 0.05kg of lecithin, and uniformly stirring and mixing to obtain a colorless and transparent polypeptide freeze-dried powder solution; s23: naturally cooling the polypeptide freeze-dried powder solution obtained in the step S22 to 38 ℃, adjusting the pH value to 6, and performing report inspection; freezing the qualified mixed solution obtained in S23 at-45 deg.C for 20min, and sublimating at-6 deg.C under vacuum degree of 30Pa for 12h to obtain white lyophilized powder with water content of less than 3%.

Comparative example 5:

this comparative example differs from example 1 in that no plant soothing agent was added in step S45 of this comparative example.

Performance testing

1. Testing the pH value of the mask:

s1: the mask compositions prepared in examples 1 to 14 and comparative examples 1 to 5, respectively, at S5 were taken in a sterile box;

s2: opening the packaging bag 1, the packaging bag 2 and the packaging bag 3, extruding the freeze-dried solvent in the packaging bag 3 into the polypeptide freeze-dried powder in the packaging bag 2, mixing uniformly, extruding into the mask liquid with membrane cloth in the packaging bag 1, standing for 2-3min to obtain a liquid to be detected;

s3: the pH of each test solution prepared in S2 was detected according to the general rules of physicochemical detection methods in the fourth chapter of physicochemical detection methods of standard QB/T2872-2017 mask and cosmetic safety specifications, i.e., the pH of the mask compositions prepared in examples 1-14 and comparative examples 1-5.

TABLE 1 pH of mask compositions prepared in examples 1-14 and comparative examples 1-5

As can be seen from table 1: the mask compositions prepared in examples 1 to 14 and comparative examples 1 to 5 each had a pH value of 6.0 to 7.0, and thus were close to the normal pH value of the facial skin, and thus were suitable for normal use on the facial skin.

2. Testing bacterial colony in freeze-dried powder:

s1: respectively putting the polypeptide freeze-dried powder prepared in S24 in examples 1-14 and comparative examples 1-5 in a beaker in an aseptic box, adding the freeze-dried solvent prepared in S33, and preparing 100mL of test solution;

s2: the total number of bacterial colonies of each test solution prepared in S1 is detected according to a microorganism detection method in chapter V of Standard QB/T2872-2017 facial mask and cosmetic safety technical Specification, namely the total number of bacterial colonies of the polypeptide freeze-dried powder prepared in examples 1-14 and comparative examples 1-5.

TABLE 2 Total number of colonies of the polypeptide lyophilized powders prepared in examples 1 to 14 and comparative examples 1 to 5

As can be seen from table 2: the total number of colonies of the polypeptide freeze-dried powders prepared in the examples 1 to 14, the comparative examples 1 to 3 and the comparative example 5 is less than or equal to 500CFU/mg, so that the microbial numbers of the polypeptide freeze-dried powders prepared in the examples 1 to 14, the comparative examples 1 to 3 and the comparative example 5 meet the specified value in the technical Specification for cosmetic safety, and the polypeptide freeze-dried powders are also suitable for sensitive muscles and damaged facial skin; however, the total colony number of the polypeptide freeze-dried powder prepared in the comparative example 4 is more than or equal to 500CFU/mg, and the microbial count of the polypeptide freeze-dried powder is higher than the specified value in the technical Specification for cosmetic safety, which indicates that the freeze-drying process is not carried out in the process of preparing the polypeptide freeze-dried powder in the comparative example 4, so that the microbial growth and propagation of the polypeptide freeze-dried powder are caused to pollute the whole polypeptide freeze-dried powder, and the activity of each material in the polypeptide freeze-dried powder.

3. Immediate hydrating test of the mask:

s1: 180 healthy adult female volunteers of the same age were collected, and the facial skin condition was: the moisture content of the facial skin is 10-20%, and the facial skin is not dry; the face skin is red, the red blood filaments are invisible, and the skin is occasionally itchy and slightly painful; the pores of the facial skin are small, and acne pits exist in one third to one half of the area of the face; one third to one half of the area of the face is hidden and visible with fine lines;

s2: dividing 180 volunteers into 18 groups of 10 persons each;

s3: before the test is started, the water content of the facial skin of each volunteer is detected and recorded;

s4: the polypeptide freeze-dried powder prepared in the comparative example 4 does not participate in detection because the microbial count is higher than the specified value in the technical Specification for cosmetic safety; the mask compositions prepared in examples 1 to 14, comparative examples 1 to 3 and comparative example 5 at S5 were provided to 18 groups of volunteers for use, respectively;

s5: respectively opening the packaging bag 1, the packaging bag 2 and the packaging bag 3 by 18 groups of volunteers, extruding the freeze-dried solvent in the packaging bag 3 into the polypeptide freeze-dried powder in the packaging bag 2, uniformly mixing, extruding into the facial mask liquid with the membrane cloth in the packaging bag 1, standing for 2-3min, taking out the membrane cloth and sticking the membrane cloth to the face, taking down the membrane cloth after 15min, and then detecting and recording the water content of the face.

Table 3 facial water content test results

By the data of table 3: the moisture contents of the faces after the face packs were smeared in examples 1 to 14 and comparative examples 1 to 3 and comparative example 5 were all "+", which shows that the facial mask packs prepared in examples 1 to 14 and comparative examples 1 to 3 and comparative example 5 all had a timely and rapid moisturizing effect on the facial skin.

The relative change values of the water contents of the embodiment 13 and the embodiment 14 are more than + 25%, which shows that the water replenishing and moisture keeping performance of the whole facial mask mixed solution is obviously improved by adding the dendrobium officinale extract.

The relative change values of the water contents of the embodiment 4-the embodiment 12 are all about + 20%, which shows that the materials such as alcohols contained in the preservative of the invention can promote the water content and active ingredients in the facial mask mixed solution to be absorbed by the facial skin, thereby improving the moisturizing and moisturizing effects of the whole facial mask mixed solution and the efficacy performance of the facial skin.

4. And testing the moisturizing, relieving, whitening and repairing of the mask:

s1: detecting the water content and the facial condition of the face of each volunteer in the 18 groups and recording;

s2: the mask compositions prepared in examples 1 to 14, comparative examples 1 to 3 and comparative example 5 at S5 were provided to 18 groups of volunteers for use, respectively;

s3: respectively opening the packaging bag 1, the packaging bag 2 and the packaging bag 3 by 18 groups of volunteers, extruding the freeze-dried solvent in the packaging bag 3 into the polypeptide freeze-dried powder in the packaging bag 2, uniformly mixing, extruding into the facial mask liquid with the membrane cloth in the packaging bag 1, standing for 2-3min, taking out the membrane cloth, attaching the membrane cloth to the face, and taking off the membrane cloth after 15 min; the above procedure was repeated for 28 days in 18 groups of volunteers, and after 28 days, the detection of the water content of the face and the detection of the condition of the face were performed again and recorded.

TABLE 4 mask evaluation criteria

Results of evaluation of facial Water content and facial Condition after 528 days in Table

As can be seen from table 5: the scoring results of the examples 1 to 14 are all more than 6 points, which shows that after 28 days, each volunteer uses the prepared facial mask mixed solution of the invention, all the aspects of the facial skin are improved after a complete metabolic cycle of the facial skin, and the moisture content of the facial skin of each example is maintained between 10% and 20%; the face skin is red, the red blood filaments are invisible, and the skin is occasionally itchy and slightly painful; the skin of the face is white, and spots are formed in one third to one half of the area of the face; the pores of the facial skin are small, and acne marks and pits exist in one third to one half of the area of the face; one third to one half of the area of the face is hidden and visible with fine lines; the facial mask mixed solution of the examples 1 to 14 can obviously improve the water content of the facial skin, can improve the redness and redness of the facial skin, has the effects of reducing pores of the facial skin and repairing acne marks and pits on the facial skin, and improves the fine lines of the facial skin to a certain extent.

Each item of the facial skin of the example 13 and the example 14 has a score of more than 6, which shows that the facial mask mixed solution of the example 13 and the example 14 has obvious conditioning and improving effects on the facial skin, and has the effects of moisturizing, relaxing, whitening and repairing the sensitive damaged facial skin after long-term use.

The scores for each item of facial skin of examples 4 to 12 were slightly lower than those of examples 13 and 14, but the evaluation results for each item of facial skin of examples 4 to 12 were higher than those of examples 1 to 3 and those of comparative examples 1 to 3 and 5, indicating that the facial mask mixtures of examples 4 to 12 were able to improve the facial skin to some extent. The scores of the items of the facial skin of the examples 7 to 9 are similar to those of the facial skin of the examples 10 to 12, wherein the evaluation results of the moisture content and the tolerance of the facial skin are relatively good, which shows that the preservative of the invention can be used for replacing the conventional chemical preservative, so that the moisture and materials in the facial mask mixed solution can be promoted to rapidly permeate into the facial skin, the moisture content of the facial skin and the effective absorption of the materials can be improved, the damage to the sensitive facial skin can be reduced to a certain extent, and the safety of the facial skin can be improved.

Compared with the scores of the items of the facial skin of the example 1, the scores of the items of the facial skin of the comparative examples 1-2 are obviously lower than the scores of the items of the facial skin of the example 1, which shows that the materials of alpha-arbutin, nonapeptide-1 and the like are cooperated with tranexamic acid to effectively inhibit the generation and diffusion of melanin, and the oxidation resistance of the materials of ascorbic acid, magnolia bark extract and the like is matched to accelerate the metabolism and the shedding of the generated melanin, so that the whitening effect on the facial skin is comprehensively improved. In addition, materials such as alpha-arbutin, nonapeptide-1 and the like also affect other items of the facial skin. The facial skin tolerance score in the comparative example 2 is low, so that the facial skin tolerance can be obviously influenced by the materials such as the ascorbic acid and the cortex magnoliae officinalis extract, and the ascorbic acid and the cortex magnoliae officinalis extract have the effects of resisting inflammation and allergy on the facial skin.

Compared with the scores of the items of the facial skin of example 1, the smoothness and elasticity of the facial skin of comparative example 3 are both significantly lower than those of example 1, which indicates that oligopeptide-5 and oligopeptide-1 can specifically stimulate the metabolism of epithelial cells and promote the growth of cells to increase the thickness of stratum corneum, so that the activities of collagen and elastin are enhanced, the facial skin can be effectively repaired and the elasticity of the facial skin is increased, the aging progress of the facial skin is delayed, and the youthful state of the facial skin is prolonged. In addition, because of the small molecular weight, oligopeptide-5 and oligopeptide-1 can be quickly and effectively absorbed by the facial skin, so that the amino acids contained in the facial skin can be replaced to participate in the saccharification reaction, the amino acids contained in the facial skin are prevented from being saccharified, and the anti-saccharification effect is further achieved; meanwhile, the molecular weight is small to a certain degree, so that the biological activity of the polypeptide is changed, and the polypeptide is easy to absorb and utilize by a human body. When the ascorbic acid, the oligopeptide-5, the oligopeptide-1 and other materials are stored in a freeze-dried powder state, the biological activity of the materials can be further improved, and the storage conditions of the materials can be optimized, so that the addition of a preservative in the polypeptide freeze-dried powder is reduced, and the safety of the skin of the face is improved. When the skin whitening and repairing liquid is used, the biological activity of each material in the polypeptide freeze-dried powder can be quickly recovered by adding the freeze-dried solvent, and each material in the polypeptide freeze-dried powder is promoted to be quickly absorbed by the skin of the face, so that the skin whitening and repairing effects of the polypeptide freeze-dried powder on the skin are further improved.

Compared with the scores of the items of the facial skin of example 1, the scores of the tolerance of the facial skin and the skin color of the facial skin in comparative example 5 are obviously lower than those of the skin tolerance of the facial skin and the skin color of the facial skin in example 1, so that the addition of the stevioside, the scutellaria root extract and the like can effectively relieve skin allergy symptoms and improve the resistance of the facial skin, and the facial skin can be effectively repaired.

The present embodiment is only for explaining the present invention, and it is not limited to the present invention, and those skilled in the art can make modifications of the present embodiment without inventive contribution as needed after reading the present specification, but all of them are protected by patent law within the scope of the claims of the present invention.

Claims

1. A polypeptide freeze-dried powder mask composition is characterized by consisting of polypeptide freeze-dried powder, a freeze-dried solvent, a mask solution and a mask cloth;

the polypeptide freeze-dried powder is prepared from the following raw materials in percentage by weight: 3.561-4.021% of mannitol, 1.56-3.21% of trehalose, 0.238-0.32% of glycine, 0.1-0.25% of creatine, 0.034-0.064% of alpha-arbutin, 0.64-0.9% of tranexamic acid, 0.02-0.06% of ascorbic acid, 0.05-0.15% of mandelic acid, 0.05-0.09% of cortex magnoliae officinalis bark extract, 0.9-1.321% of nonapeptide-1, 0.06-0.1% of oligopeptide-5, 0.36-0.53% of oligopeptide-1, 0.02-0.05% of lecithin and the balance of water to 100%;

2. The polypeptide lyophilized powder mask composition of claim 1, wherein the plant soothing agent is prepared from the following raw materials: stevioside, scutellaria root extract, flavescent sophora root extract and licorice root extract.

3. The polypeptide lyophilized powder mask composition of claim 1, wherein the dendrobium extract is a dendrobium officinale extract.

4. The polypeptide lyophilized powder mask composition of claim 1, wherein the preservative composition is prepared from the following components in percentage by weight of 1: 10:(10-20): (10-20): (20-40) ethylhexylglycerin: p-hydroxyacetophenone: caprylyl hydroximic acid, glyceryl caprylate and 1, 3-propylene glycol.

5. The preparation method of the polypeptide freeze-dried powder mask composition is characterized by comprising the following steps:

s1: weighing materials: weighing the materials in proportion;

s2: preparing polypeptide freeze-dried powder:

s21: heating water to 80-90 deg.C;

s3: preparation of freeze-dried solvent:

s31: heating water to 80-90 deg.C;

s4: preparing mask liquid:

s5: assembling:

6. The method for preparing a polypeptide lyophilized powder mask composition according to claim 5, wherein the vacuum freeze-drying conditions in S24 are as follows: the freezing temperature is-35 deg.C to-45 deg.C, and the freezing time is 20-30 min; the sublimation temperature is-8-0 deg.C, the vacuum degree is 20-30Pa, and the sublimation time is 8-16 h; the water content of the freeze-dried powder after vacuum freeze-drying is less than or equal to 3 percent.

7. The method for preparing polypeptide lyophilized powder mask composition as claimed in claim 5, wherein the viscosity of the mask solution prepared in S45 is in the range of 1000-1500 mPa-S.

8. The use method of the polypeptide lyophilized powder mask composition according to claim 1, which comprises the following steps: opening the packaging bag 1, the packaging bag 2 and the packaging bag 3, extruding the freeze-dried solvent in the packaging bag 3 into the polypeptide freeze-dried powder in the packaging bag 2, mixing uniformly, extruding into the mask liquid with the membrane cloth in the packaging bag 1, standing for 2-3min, and taking out the membrane cloth for use.