CN111617021B

CN111617021B - Toothpaste for resisting oral helicobacter pylori

Info

Publication number: CN111617021B
Application number: CN202010648454.5A
Authority: CN
Inventors: 刘滨; 方浩; 刘诗慧; 王俊嘉
Original assignee: Shanghai Mange Technology Co ltd
Current assignee: Shanghai Mange Technology Co ltd
Priority date: 2020-07-07
Filing date: 2020-07-07
Publication date: 2022-07-22
Anticipated expiration: 2040-07-07
Also published as: CN111617021A

Abstract

The application discloses an anti-helicobacter pylori toothpaste in oral cavity, which comprises: 1-10 wt% of an effective component, 25-35 wt% of water, 8-13 wt% of an abrasive, 45-55 wt% of a humectant, 2-3 wt% of a thickening agent, 1-3 wt% of a foaming agent, 0.5-1.5 wt% of essence and 2-4 wt% of a taste modifier; the effective components comprise Olibanum, propolis extract, oleum Caryophylli, grapefruit seed extract and tea tree essential oil. The toothpaste has a helicobacter pylori killing rate of over 99 percent, and has an effective helicobacter pylori inhibiting period of 12 hours.

Description

Toothpaste for resisting oral helicobacter pylori

Technical Field

The application relates to an anti-oral helicobacter pylori toothpaste, belonging to the technical field of foods and cosmetics.

Background

Helicobacter pylori is a bacterium that is helicoidal, micro-anaerobic, and has very strict requirements for growth conditions. Helicobacter pylori disease, which is the only microorganism species known to survive in human stomach, is the disease of gastritis, peptic ulcer, lymphoproliferative gastric lymphoma and the like, which is first isolated from biopsy tissue of gastric mucosa of a patient with chronic active gastritis in 1983.

In 2017, 10 and 27, the international cancer research institution of the world health organization publishes a carcinogen list for preliminary reference, and helicobacter pylori (infection) is in a carcinogen list. Currently, antibiotic therapy is very effective in treating helicobacter pylori, but there is still the possibility of recurrent infection after treatment. Due to the eating habits of the Chinese catering system, various bacteria in the oral cavity can easily pass through tableware or food during the catering process and are mutually transmitted to cause cross infection.

Olibanum is a perfume for preventing halitosis, and can inhibit activity of pathogenic bacteria of periodontal disease and dental caries and fungi. However, the addition amount of the frankincense is usually more than 10wt% to show antibacterial property in human body due to mild action of the frankincense. The frankincense is an oily component, and the foaming performance of the toothpaste can be affected by independently adding too much frankincense into the toothpaste, so that the toothpaste body can be seriously layered and separated out in the storage process, and the sale and use are affected.

Meanwhile, the frankincense has obvious characteristic smell, and the characteristic smell is too strong after the excessive frankincense is added, so that the frankincense is unacceptable to most users and influences the long-term normal use of the product when being used as toothpaste needing to be taken in the mouth.

Disclosure of Invention

The application provides toothpaste for resisting helicobacter pylori in the oral cavity, which is used for solving the problems that the storage performance of the toothpaste is reduced and the smell is unacceptable after too much frankincense is added in the prior art; limited anti-helicobacter pylori effect and short bacteriostatic time after tooth brushing.

The application provides an anti-helicobacter pylori toothpaste for oral cavity, which comprises: 1-10 wt% of effective components, 25-35 wt% of water, 8-13 wt% of an abrasive, 45-55 wt% of a humectant, 2-3 wt% of a thickening agent, 1-3 wt% of a foaming agent, 0.5-1.5 wt% of essence and 2-4 wt% of a taste modifier;

the effective components comprise Olibanum, propolis extract, oleum Caryophylli, grapefruit seed extract and tea tree essential oil.

According to the application, after the frankincense is mixed with the propolis extract, the clove oil, the grapefruit seed extract and the tea tree essential oil, the solubility of the frankincense in the toothpaste can be improved, and meanwhile, the effect of effectively killing helicobacter pylori is guaranteed; meanwhile, the propolis extract, the clove oil, the grapefruit seed extract and the tea tree essential oil can also cooperate with the frankincense to play a long-acting antibacterial effect, so that an adverse growth environment for helicobacter pylori is continuously formed in the oral cavity after tooth brushing, and the antibacterial effect of the toothpaste on the oral cavity is improved.

The abrasive agent used in the toothpaste formula can be at least one of calcium carbonate, calcium hydrophosphate and precipitated silica; the humectant can be at least one of glycerol, sorbitol and propylene glycol; the thickener can be at least one of sodium carboxymethylcellulose, microcrystalline cellulose, hydroxyethyl cellulose, carbomer resin, xanthan gum (xanthan gum), modified guar gum, and carrageenan; the foaming agent can be sodium dodecyl sulfate, sodium lauroyl sarcosinate, and cocamidopropyl betaine; flavor and taste modifiers may be those commonly used in the art.

Preferably, the composition is prepared from: 10wt% of effective component, 25wt% of water, 10wt% of abrasive, 45wt% of humectant, 2wt% of thickener, 3wt% of foaming agent, 1.5wt% of essence, 3.5wt% of taste modifier and a proper amount of auxiliary components.

The proper amount of auxiliary materials refers to the types of auxiliary materials required in the existing toothpaste manufacturing process in the field; the addition amount of the auxiliary materials can be added according to the existing requirements in the field. And will not be described in detail herein.

Preferably, the composition is prepared from: 10wt% of effective component, 27wt% of water, 8wt% of abrasive, 45wt% of humectant, 2wt% of thickener, 3wt% of foaming agent, 1.5wt% of essence and 3.5wt% of taste modifier. The toothpaste prepared according to the formula does not generate chromatography and precipitation after being stored for 2 months.

Preferably, the effective components comprise 30-80 wt% of frankincense, 10-30 wt% of propolis extract, 1-10 wt% of clove oil, 5-20 wt% of grapefruit seed extract and 1-10 wt% of tea tree essential oil.

The effective components obtained after mixing according to the formula can effectively cover the adverse effect of characteristic odor on the sense of a user caused by the large addition of the frankincense, wherein the odor emitted by the clove oil and the tea tree essential oil can be mixed with the characteristic fragrance of the frankincense to form the odor which is good for the user to feel, so that the improvement on the odor of the frankincense is realized without reducing the using amount of the frankincense, and the acceptable degree of the user is improved.

Preferably, the effective components comprise 75wt% of frankincense, 10wt% of propolis extract, 5wt% of clove oil, 5wt% of grapefruit seed extract and 5wt% of tea tree essential oil.

After the effective components obtained by mixing according to the proportion are added into other components, the compatibility of a large amount of frankincense with other components can be improved, and the problem of precipitation and delamination is avoided.

Preferably, the effective components comprise 70wt% of frankincense, 10wt% of propolis extract, 10wt% of clove oil, 5wt% of grapefruit seed extract and 5wt% of tea tree essential oil. The effective components obtained after mixing according to the proportion are added into other components, and the obtained toothpaste has better long-acting antibacterial effect.

Preferably, the composition is prepared from: 10 weight percent of effective component, 25 weight percent of water, 10 weight percent of abrasive, 45 weight percent of humectant, 2 weight percent of thickening agent, 3 weight percent of foaming agent, 1.5 weight percent of essence and 3.5 weight percent of taste modifier.

The toothpaste is prepared by the following steps:

1) adding sorbitol and glycerin (humectant) into a vacuum paste making machine, adding microcrystalline cellulose and carrageenan serving as a thickening agent, fully mixing, adding most deionized water, mixing uniformly, and sequentially adding xylitol and sucralose which are dispersed and dissolved by a small part of deionized water serving as a taste modifier, effective components, precipitated silicon dioxide (friction agent) and sodium lauroyl sarcosine serving as a foaming agent under the vacuum stirring state;

2) and (2) stirring the paste obtained in the step 1) at a high speed in a vacuum state, fully and uniformly mixing, finally adding essence, stirring at a high speed in a vacuum state, fully emulsifying and uniformly mixing to obtain the precipitated silica type toothpaste.

The beneficial effect that this application can produce includes:

1) the application provides an anti oral cavity helicobacter pylori's toothpaste, regard frankincense, propolis extract, clove oil, grapefruit seed extract, tea tree essential oil as main active ingredient, after adding according to this, can improve the storage duration of toothpaste after adding a large amount of frankincense, ester solubility frankincense can fully melt into other ingredients of toothpaste, form the water emulsion cladding body, thereby avoid the separation and layering problem of frankincense, can also effectively improve the smell of toothpaste after adding 10wt% frankincense simultaneously, make user's sense organ appraisal score reach 10 minutes, be favorable to the user to persist the use for a long time and reach effective antibacterial effect.

2) The toothpaste for resisting oral helicobacter pylori provided by the application has the killing rate of more than 99% on helicobacter pylori, porphyromonas gingivalis and streptococcus mutans, and meanwhile, the effective duration of bacteriostasis on the helicobacter pylori reaches 12 hours.

Drawings

FIG. 1 is a photograph of a helicobacter pylori suspension after 3min contact with a toothpaste sample 1 in example 4 of the present application;

FIG. 2 is a photograph of the bacterial suspension of Porphyromonas gingivalis in example 4 of the present application after 3min contact with toothpaste sample 1;

FIG. 3 is a photograph of a bacterial suspension of Streptococcus mutans in example 4 of the present application after contacting with toothpaste sample 1 for 3 min;

FIG. 4 is a photograph of a helicobacter pylori bacterial suspension in example 4 of the present application after contacting the suspension with a blank for 3 min;

FIG. 5 is a photograph of a bacterial suspension of Porphyromonas gingivalis in example 4 of the present application after 3min contact with a blank;

FIG. 6 is a photograph of a bacterial suspension of Streptococcus mutans in example 4 of the present application after contacting a blank for 3 min;

Detailed Description

The present application will be described in detail with reference to examples, but the present application is not limited to these examples.

The application provides an anti-oral helicobacter pylori toothpaste, which comprises:

examples

Unless otherwise specified, the raw materials, solvents and auxiliaries in the examples of the present application were purchased commercially and were not processed.

EXAMPLES 1-3 toothpaste samples for anti-helicobacter pylori in oral cavity 1-3

The toothpaste is prepared by the following steps:

2) and (2) stirring the paste obtained in the step 1) at a high speed in a vacuum state, fully and uniformly mixing, finally adding essence, stirring at a high speed in a vacuum state, fully emulsifying and uniformly mixing to obtain the precipitated silica type toothpaste sample 1-3.

The raw material formulations used in examples 1 to 3 are shown in Table 1.

TABLE 1

Comparative example 1:

the difference from example 1 is that: effective components of mastic gum 75wt%, clove oil 15 wt%, grapefruit seed extract 10wt%, to obtain toothpaste sample D1.

Comparative example 2:

the difference from example 1 is that: effective components of 80wt% of frankincense, 15 wt% of grapefruit seed extract composition and 5wt% of tea tree essential oil to obtain toothpaste sample D2.

Example 4 bacteriostatic experiments on toothpaste samples 1-3 and D1-2

According to the American dental Association, it is found that a suitable time for a person to brush his teeth is 3-5min, 3min and 5min being selected as the time points for the experiment. Normally, the single use amount of the toothpaste is 3-5g, and 3g of toothpaste is selected as the experimental condition.

The concentration of the experimental group in the bacterial liquid is 5.1 x 10⁵And 3g of toothpaste samples 1-3 are mixed into the CFU/mL bacterial solution for experiment, and the antibacterial rate and the natural mortality rate are respectively measured after 3min and 5min of treatment.

The bacterial liquid is helicobacter pylori, porphyromonas gingivalis and streptococcus mutans bacterial liquid.

In addition, a control group is arranged, and the treatment group without the toothpaste sample 1-3 is used as a first control group; toothpaste D1-D2 obtained by coating comparative examples were used as a second control group; bacterial survival was determined under the same conditions as described above.

Meanwhile, blank samples are respectively set for each bacterial suspension, and the blank samples are added with deionized water.

The results obtained are shown in tables 2 to 4 below:

TABLE 2 toothpaste samples 1-3, D1-2 helicobacter pylori bacteriostasis experiment results

TABLE 3 toothpaste samples 1-3, D1-2 bacteriostatic test results of Porphyromonas gingivalis

TABLE 4 antibacterial test results of Streptococcus mutans of toothpaste samples 1-3 and D1-2

As can be seen from tables 2 to 4, the toothpaste prepared by adopting the formula provided by the application can effectively inhibit the above 3 kinds of bacteria in the oral cavity of the human body, and can achieve better bacteria killing in the process of tooth brushing.

As can be seen by respectively comparing fig. 1-6, the toothpaste sample provided by the application can effectively inhibit the growth of helicobacter pylori, porphyromonas gingivalis and streptococcus mutans.

Example 5 bacteriostatic duration test of toothpaste samples 1-3 and D1-2

10mL of the prepared helicobacter pylori suspension in example 4 is taken, mixed with 250mL of the prepared helicobacter pylori medium respectively, shaken evenly, poured into a sterilized culture dish, and cultured in a covering manner until the surface of the medium forms bacterial colonies, wherein the area of the bacterial colonies is more than 50% of the surface of the medium. Preparing toothpaste samples 1-3 and D1-2 into a solution with the concentration of 50 wt%.

After the surface of each culture medium is punched, adding 120 microliters of toothpaste solution into each hole, continuously culturing and recording the bacteriostasis failure time, and observing the diameter of a primary bacteriostasis ring after culturing for 1 hour; the bacteriostasis failure time is as follows: during the observation, the diameter of the formed inhibition zone is reduced by more than 5mm compared with the diameter of the inhibition zone in the last hour. The results are shown in Table 5.

TABLE 5 experiment results of bacteriostatic duration of toothpaste samples 1-3 and D1-2

Strain	Sample 1	Sample 2	Sample 3	Sample D1	Sample D2
						Helicobacter pylori	12h	12h	12h	10h	10h

As can be seen from the table, the toothpaste formula provided by the application can effectively prolong the bacteriostasis duration of helicobacter pylori. The toothpaste obtained in the comparative examples had a lower duration of inhibition against helicobacter pylori than the toothpaste provided herein.

Example 6 storage time of toothpaste samples 1-3 and D1-2

Filling the toothpaste samples 1-3 and D1-2 into paste bodies, storing at normal temperature and pressure for 2 months, and opening and observing the paste state of a bottle mouth; and extruding the paste, standing for 2 hours, and observing whether layering or solvent precipitation occurs. The results obtained are shown in the following table:

TABLE 6 toothpaste samples 1-3 and D1-2 after 2 months of storage status

As can be seen from the above table, the toothpaste provided by the application can effectively inhibit the precipitation of the water-soluble solvent in the toothpaste during the storage process after the frankincense is added in a large amount; according to the formula, the addition amount of water in the toothpaste is 25-35 wt%, and by adding the propolis extract, the clove oil, the grapefruit seed extract and the tea tree essential oil, an oil-in-water stable result can be formed in the stirring process, so that the problem of solvent precipitation in the toothpaste is avoided, and the state stability of the toothpaste is improved.

The result of D1 shows that when propolis extract and tea tree essential oil are not added, the effective fusion of frankincense and other components of toothpaste can not be promoted, and the problems of liquid seepage and layering can not be avoided in the storage process;

from the result of D2, it was found that the problems of delamination and liquid separation occurred even when the propolis component and clove oil were not added.

The results obtained from D1-2 show that the propolis extract can effectively dissolve frankincense while avoiding separation and delamination of the frankincense during storage after being mixed with ester components such as clove oil and tea tree essential oil, which may be related to the fact that the propolis extract contains fat-soluble components and water-soluble components, and the propolis can partially emulsify the frankincense after being added.

Example 7 sensory evaluation of toothpaste samples 1 to 3 and D1 to 2

50 test persons were selected from 10 persons per group, toothpaste samples 1-3 and D1-2 were used for 3g each, after brushing teeth for 5min, sensory evaluation of toothpaste was scored, and taste and odor of toothpaste were responded to according to the criteria in Table 7, and the results are shown in Table 8.

TABLE 7 sensory evaluation criteria Table

Standard of reference	Score of
		Has no characteristic milk fragrance smell	5
Shows part of characteristic odor of frankincense	3
		The characteristic smell of frankincense is strong	1
No milk fragrance characteristic taste	5
		Display part of characteristic flavor of mastic	3
The Olibanum has strong characteristic taste	1

TABLE 8 sensory evaluation results for toothpaste samples 1-3 and D1-2

As can be seen from table 8, the toothpaste prepared by the method provided by the present application does not show mastic characteristics in taste and odor, which is beneficial to improving user acceptance, and still can effectively avoid the influence of mastic on unpleasant odor and taste under the condition of adding more mastic.

From the results obtained in D1-2, it was found that in the absence of propolis extract, it was difficult to effectively neutralize the characteristic odor and taste of mastic by adding only clove oil or tea tree essential oil. The characteristic odor and taste of the frankincense need to be simultaneously and synergistically exerted by the propolis extract, the clove oil and the tea tree essential oil.

Reference throughout this specification to "one embodiment," "another embodiment," "an embodiment," "a preferred embodiment," or the like, means that a particular feature, structure, or characteristic described in connection with the embodiment is included in at least one embodiment described generally in this application. The appearances of the same phrase in various places in the specification are not necessarily all referring to the same embodiment. Further, when a particular feature, structure, or characteristic is described in connection with any embodiment, it is submitted that it is within the scope of the disclosure to effect such feature, structure, or characteristic in connection with other embodiments.

Although the present application has been described herein with reference to a number of illustrative embodiments thereof, it should be understood that numerous other modifications and embodiments can be devised by those skilled in the art that will fall within the spirit and scope of the principles of this disclosure. More specifically, various variations and modifications are possible in the component parts and/or arrangements of the subject combination arrangement within the scope of the disclosure and claims of this application. In addition to variations and modifications in the component parts and/or arrangements, other uses will also be apparent to those skilled in the art.

Claims

1. An anti-helicobacter pylori toothpaste for oral cavity, which is characterized by comprising: 1-10 wt% of effective components, 25-35 wt% of water, 8-13 wt% of an abrasive, 45-55 wt% of a humectant, 2-3 wt% of a thickening agent, 1-3 wt% of a foaming agent, 0.5-1.5 wt% of essence and 2-4 wt% of a taste modifier;

the effective components comprise frankincense, propolis extract, clove oil, grapefruit seed extract and tea tree essential oil;

the effective components comprise 30-80 wt% of frankincense, 10-30 wt% of propolis extract, 1-10 wt% of clove oil, 5-20 wt% of grapefruit seed extract and 1-10 wt% of tea tree essential oil.

2. The oral helicobacter pylori resistant toothpaste according to claim 1, which is prepared from: 10wt% of effective component, 27wt% of water, 8wt% of abrasive, 45wt% of humectant, 2wt% of thickener, 3wt% of foaming agent, 1.5wt% of essence and 3.5wt% of taste modifier.

3. The toothpaste for resisting oral helicobacter pylori according to claim 1, wherein the effective components consist of 75wt% of frankincense, 10wt% of propolis extract, 5wt% of clove oil, 5wt% of grapefruit seed extract and 5wt% of tea tree essential oil.

4. The toothpaste for resisting oral helicobacter pylori according to claim 1, wherein the effective components consist of 70wt% of frankincense, 10wt% of propolis extract, 10wt% of clove oil, 5wt% of grapefruit seed extract and 5wt% of tea tree essential oil.

5. The oral helicobacter pylori resistant toothpaste according to claim 1, which is prepared from: 10 weight percent of effective component, 25 weight percent of water, 10 weight percent of abrasive, 45 weight percent of humectant, 2 weight percent of thickener, 3 weight percent of foaming agent, 1.5 weight percent of essence and 3.5 weight percent of taste modifier.