CN111607586A - Immobilized desulfurization strain and method for treating gas containing sulfur dioxide - Google Patents
Immobilized desulfurization strain and method for treating gas containing sulfur dioxide Download PDFInfo
- Publication number
- CN111607586A CN111607586A CN202010466081.XA CN202010466081A CN111607586A CN 111607586 A CN111607586 A CN 111607586A CN 202010466081 A CN202010466081 A CN 202010466081A CN 111607586 A CN111607586 A CN 111607586A
- Authority
- CN
- China
- Prior art keywords
- desulfurization
- immobilized
- strain
- solution
- gas
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
- 238000006477 desulfuration reaction Methods 0.000 title claims abstract description 77
- 230000023556 desulfurization Effects 0.000 title claims abstract description 77
- 238000000034 method Methods 0.000 title claims abstract description 21
- RAHZWNYVWXNFOC-UHFFFAOYSA-N Sulphur dioxide Chemical compound O=S=O RAHZWNYVWXNFOC-UHFFFAOYSA-N 0.000 title claims abstract description 19
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 claims abstract description 17
- 229910052717 sulfur Inorganic materials 0.000 claims abstract description 15
- 239000011593 sulfur Substances 0.000 claims abstract description 15
- 238000001914 filtration Methods 0.000 claims abstract description 14
- 238000002360 preparation method Methods 0.000 claims abstract description 8
- 230000001580 bacterial effect Effects 0.000 claims abstract description 6
- 238000011084 recovery Methods 0.000 claims abstract description 6
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 55
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 51
- 239000000377 silicon dioxide Substances 0.000 claims description 31
- 239000000243 solution Substances 0.000 claims description 29
- 238000010521 absorption reaction Methods 0.000 claims description 26
- 239000007788 liquid Substances 0.000 claims description 24
- 229920001661 Chitosan Polymers 0.000 claims description 23
- 235000012239 silicon dioxide Nutrition 0.000 claims description 19
- 239000007787 solid Substances 0.000 claims description 19
- 239000000126 substance Substances 0.000 claims description 17
- 239000007864 aqueous solution Substances 0.000 claims description 16
- 239000002131 composite material Substances 0.000 claims description 14
- 239000002994 raw material Substances 0.000 claims description 13
- 238000001035 drying Methods 0.000 claims description 11
- 239000011343 solid material Substances 0.000 claims description 10
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 claims description 6
- 238000005303 weighing Methods 0.000 claims description 6
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L magnesium chloride Substances [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 claims description 5
- 229910001629 magnesium chloride Inorganic materials 0.000 claims description 5
- FGIUAXJPYTZDNR-UHFFFAOYSA-N potassium nitrate Inorganic materials [K+].[O-][N+]([O-])=O FGIUAXJPYTZDNR-UHFFFAOYSA-N 0.000 claims description 5
- 229910000030 sodium bicarbonate Inorganic materials 0.000 claims description 5
- UIIMBOGNXHQVGW-UHFFFAOYSA-M sodium bicarbonate Substances [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 claims description 5
- 238000005406 washing Methods 0.000 claims description 5
- 238000012258 culturing Methods 0.000 claims description 4
- 230000010355 oscillation Effects 0.000 claims description 3
- 241000894006 Bacteria Species 0.000 claims description 2
- 238000007254 oxidation reaction Methods 0.000 claims description 2
- 238000001291 vacuum drying Methods 0.000 claims description 2
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 claims 1
- 239000002250 absorbent Substances 0.000 claims 1
- 230000002745 absorbent Effects 0.000 claims 1
- 238000006243 chemical reaction Methods 0.000 abstract description 3
- 230000000694 effects Effects 0.000 abstract description 2
- 239000000463 material Substances 0.000 abstract description 2
- 244000005700 microbiome Species 0.000 abstract description 2
- 238000000746 purification Methods 0.000 abstract description 2
- 239000003513 alkali Substances 0.000 abstract 1
- -1 sulfur ions Chemical class 0.000 abstract 1
- 239000007789 gas Substances 0.000 description 21
- 238000011049 filling Methods 0.000 description 7
- 239000002245 particle Substances 0.000 description 7
- 241000605268 Thiobacillus thioparus Species 0.000 description 5
- 239000012153 distilled water Substances 0.000 description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
- 238000012545 processing Methods 0.000 description 4
- 239000012086 standard solution Substances 0.000 description 4
- BPQQTUXANYXVAA-UHFFFAOYSA-N Orthosilicate Chemical compound [O-][Si]([O-])([O-])[O-] BPQQTUXANYXVAA-UHFFFAOYSA-N 0.000 description 3
- 241000605118 Thiobacillus Species 0.000 description 3
- 239000000969 carrier Substances 0.000 description 3
- 238000005265 energy consumption Methods 0.000 description 3
- 230000007613 environmental effect Effects 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 239000008187 granular material Substances 0.000 description 3
- 230000003301 hydrolyzing effect Effects 0.000 description 3
- BHTJEPVNHUUIPV-UHFFFAOYSA-N pentanedial;hydrate Chemical compound O.O=CCCCC=O BHTJEPVNHUUIPV-UHFFFAOYSA-N 0.000 description 3
- 239000011347 resin Substances 0.000 description 3
- 229920005989 resin Polymers 0.000 description 3
- 238000012216 screening Methods 0.000 description 3
- 150000001875 compounds Chemical class 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000003344 environmental pollutant Substances 0.000 description 2
- 231100000719 pollutant Toxicity 0.000 description 2
- 230000002210 biocatalytic effect Effects 0.000 description 1
- 238000006065 biodegradation reaction Methods 0.000 description 1
- 230000003197 catalytic effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 239000002440 industrial waste Substances 0.000 description 1
- 238000006053 organic reaction Methods 0.000 description 1
- 238000007670 refining Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000010802 sludge Substances 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
- 239000002351 wastewater Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/14—Enzymes or microbial cells immobilised on or in an inorganic carrier
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D53/00—Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols
- B01D53/34—Chemical or biological purification of waste gases
- B01D53/46—Removing components of defined structure
- B01D53/48—Sulfur compounds
- B01D53/50—Sulfur oxides
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D53/00—Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols
- B01D53/34—Chemical or biological purification of waste gases
- B01D53/46—Removing components of defined structure
- B01D53/48—Sulfur compounds
- B01D53/50—Sulfur oxides
- B01D53/501—Sulfur oxides by treating the gases with a solution or a suspension of an alkali or earth-alkali or ammonium compound
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D53/00—Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols
- B01D53/34—Chemical or biological purification of waste gases
- B01D53/74—General processes for purification of waste gases; Apparatus or devices specially adapted therefor
- B01D53/84—Biological processes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/10—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a carbohydrate
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D2251/00—Reactants
- B01D2251/95—Specific microorganisms
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/20—Air quality improvement or preservation, e.g. vehicle emission control or emission reduction by using catalytic converters
Landscapes
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biomedical Technology (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Environmental & Geological Engineering (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- General Chemical & Material Sciences (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Analytical Chemistry (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Inorganic Chemistry (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
Abstract
The invention relates to an immobilized desulfurization strain and a method for treating gas containing sulfur dioxide, which mainly aims at SO2A gas. Wherein the immobilized desulfurization strain is obtained by the preparation of an immobilized carrier and the immobilized culture of desulfurization microorganisms; the process of the treatment method comprises the steps of absorbing sulfur-containing gas by alkali liquor to obtain sulfur-rich solution, and obtaining S by anaerobic reduction2‑Solution, placing the immobilized desulfurization bacterial strain into a biological trickling filter, and using the S2‑The solution is filtered by the drop filtration,fully contact and react with the immobilized strains, and oxidize the sulfur ions in the immobilized strains to generate elemental sulfur for recovery. The method has the advantages of simple preparation of the used materials, clear operation flow, good purification effect, high conversion efficiency of sulfur-polluted gas and continuous treatment capability.
Description
Technical Field
The invention relates to treatment of industrial waste gas, in particular to an immobilized desulfurization strain and a method for treating gas containing sulfur dioxide.
Background
The sulfur-containing substances in the fossil energy can be extracted as SO in the processes of refining, using and the like2The emission in the same form has great influence on the quality of the atmospheric environment, and is considered as a main cause of frequent haze weather. The desulfurization process has been developed for a long time, and at present, two processes, namely wet desulfurization and dry desulfurization, are mainly adopted, and have respective advantages and disadvantages, and the main problems can be roughly summarized into the problems of high energy consumption, difficult treatment of wastewater and sludge, secondary pollution and the like.
With the strictness of environmental regulations and the improvement of environmental awareness of the whole population, the development of novel desulfurization technology with low energy consumption, high efficiency and environmental friendliness is not slow enough. The biocatalytic conversion of sulfur-containing compounds provides a new direction for research and application in this field. Biological desulfurization is a new technology for removing sulfur-containing pollutants and recovering elemental sulfur by using microorganisms at normal temperature and normal pressure, and has the advantages of high catalytic efficiency, low energy consumption and difficult secondary pollution to the environment. Elemental sulfur obtained by biodegradation of sulfur-containing compounds can be used as a raw material for processing and utilization, waste is turned into wealth, and SO is treated2And the sulfur-containing pollutants can be recycled in a green environment.
Disclosure of Invention
The invention aims to overcome the defects in the prior art and provide an immobilized desulfurization strain, and the invention also aims to provide a method for treating sulfur dioxide-containing gas by using the immobilized desulfurization strain, so that the sulfur dioxide-containing gas is effectively purified and treated, and the aims of saving energy, reducing emission and protecting the environment are fulfilled.
The technical scheme of the invention is as follows: an immobilized desulfurization strain is characterized by being prepared by the following method, and the specific steps are as follows:
A. preparation of immobilization support
Weighing dried silicon dioxide, and putting the silicon dioxide into a standard chitosan acetic acid aqueous solution, wherein the ratio of the silicon dioxide to the chitosan acetic acid aqueous solution is 20-50 g/L; after oscillation for 4-5 h, filtering out solids, and drying in vacuum; placing the dried solid material into a glutaraldehyde aqueous solution, oscillating for 2-3 h, filtering out the solid, repeatedly washing, and vacuum drying to obtain a chitosan-silicon dioxide composite material;
B. immobilization of desulfurization strains
And (2) putting the prepared chitosan-silicon dioxide composite material into a desulfurization strain culture solution, wherein the mass-volume ratio of the chitosan-silicon dioxide composite material to the desulfurization strain culture solution is 0.1-0.2 g/mL, oscillating the chitosan-silicon dioxide composite material in a shaker at the temperature of 25-30 ℃ and the rotating speed of 120-180 r/min for 3-5 h, filtering out a solid material, and putting the solid material into an incubator at the temperature of 25-30 ℃ for culturing for 5-8 h to obtain the immobilized desulfurization strain.
Preferably, the drying condition of the silicon dioxide in the step A is 80-90 ℃, and the drying time is 2-3 h.
Preferably, the diameter of the silicon dioxide particles in the step A is 0.3-0.5 cm; the solute components of the standard chitosan acetic acid aqueous solution are as follows: 1-2 g/L of acetic acid and 1-1.5 g/L of chitosan; the solute component of the glutaraldehyde aqueous solution is as follows: 0.1-0.5 g/L glutaraldehyde.
The desulfurization strain in the step B is a subject group of the inventor, and the disclosure number of the desulfurization strain is as follows: CN109207467, application number: 2018113727506 patent, the Thiobacillus thioparus having the accession number CGMCC No.12756 at the depository, and the Latin scientific name Thiobacillus thioparus.
Preferably, the number of viable bacteria of the strain in the culture solution of the desulfurization strain in the step B is 1.0 × 109～1.5×109one/mL.
The invention also provides a method for treating sulfur dioxide-containing gas by using the immobilized desulfurization strain, which comprises the following specific steps:
(1)SO2absorption and reduction of gas: SO (SO)2The raw material gas enters from the bottom of the anaerobic absorption tower and reacts with the circulating alkaline absorption liquid in the tower to generate SO3 2-/SO4 2-The solution and the absorption liquid are subjected to anaerobic reduction treatment in a tower to obtain S2-A solution;
(2)S2-oxidation and S recovery of the solution: s in step (1)2-The solution flows in from the top of the biotrickling filter packed with the immobilized desulfurization bacterial strain, S when passing through the immobilized desulfurization bacterial strain2-Absorbed by the desulfurization strains, biologically metabolized to generate sulfur simple substance, precipitated, settled and filtered to obtain solid sulfur simple substance.
Preference is given to SO in step (1)2In the raw material gas of (2)2The volume percentage content of the (B) is 0.1 to 3 percent.
Preferably, in the step (1), the alkaline absorption liquid comprises the following components: KNO31～2g/L，NaHCO33～5g/L，MgCl20.1～0.5g/L，NH4Cl0.5～1g/L。
Preference is given to SO in step (1)2The flow of the gas entering the raw material gas is controlled to be 200-1000L/h; the gas-liquid ratio of the alkaline absorption liquid to the alkaline absorption liquid is controlled to be 15-30.
Preferably, the filling volume ratio of the immobilized desulfurization strain in the biological trickling filter is 10-15%; the optimal system operating temperature is 25-30 ℃; the running time is generally about 24 h.
Has the advantages that:
the immobilized biological desulfurization method combines the inorganic absorption unit and the biological organic reaction unit to realize the continuous biological desulfurization treatment. The material used in the invention has the advantages of simple preparation, good stability, clear operation flow of the biological desulfurization treatment method, good purification effect and high conversion efficiency of sulfur-polluted gas.
Detailed Description
The present invention is further explained by the following examples, which are not intended to limit the present invention in any way.
Example 1:
1. preparation of an immobilization carrier:
using macroporous resin as fixed template, burning and hydrolyzing silicon dioxide particles by silicate, and screening SiO with particle size of 0.3cm2As a ready-to-use granule. Preparing a standard chitosan acetic acid aqueous solution: weighing 1g of acetic acid and 1g of chitosan, adding distilled water for dissolving, transferring to a 1000mL volumetric flask, and fixing the volume with distilled waterAnd (5) obtaining the required standard solution when the standard solution reaches the scale mark. 20g of the silica is weighed out and dried at 80 ℃ for 2h, then is put into 800mL of chitosan acetic acid aqueous solution, is shaken at room temperature for 4h, then the solid is filtered out and is dried in vacuum. And (3) putting the dried solid into 0.1g/L glutaraldehyde water solution, oscillating for 2h, filtering out the solid, repeatedly washing and drying in vacuum to obtain the chitosan/silicon dioxide composite material, and repeating the steps to obtain a large number of immobilized carriers.
2. Immobilization of desulfurization strains
10g of the prepared chitosan/silicon dioxide composite material is put into 100mL of culture solution of desulfurization strain (thiobacillus thioparus with the registration number of CGMCC No.12756 in the preservation center and the Latin scientific name of Thiobacillus)9Shaking in a shaker at 25 deg.C and rotation speed of 120r/min for 3 hr, filtering out solid material, and culturing in incubator at 25 deg.C for 5 hr to obtain immobilized desulfurization strain.
3. Biological desulfurization by immobilized desulfurization strain
Filling the prepared immobilized desulfurization strain into a biological trickling filter, wherein the filling volume ratio of the immobilized desulfurization strain is 10%, and then performing a biological desulfurization experiment, wherein the specific operation flow is as follows: 200L/h 0.1% volSO2The raw material gas enters from the bottom of the anaerobic absorption tower, the gas-liquid ratio is controlled to be 15, and the raw material gas reacts with alkaline absorption liquid in the tower to generate SO3 2-/SO4 2-The solution and the alkaline absorption liquid mainly comprise the following components: KNO31g/L，NaHCO33g/L，MgCl20.1g/L，NH4Cl0.5g/L. The absorption liquid is treated by anaerobic reduction in the tower to obtain S2-A solution; s2-The solution flows in from the top of the biological trickling filter tower, and S passes through the immobilized desulfurization strain2-Absorbed by the desulfurization strains, metabolized to generate S elementary substance, precipitated, settled and filtered to obtain solid sulfur elementary substance. The whole device runs stably for 24h, and the temperature is controlled to be 25 ℃.
4. Processing the results
Through analysis of purified gas and analysis of S elementary substance products, the biological desulfurization efficiency of the scheme is about 92%, and the recovery rate of the S elementary substance is about 78%.
Example 2:
1. preparation of an immobilization carrier:
using macroporous resin as fixed template, burning and hydrolyzing silicon dioxide particles by silicate, and screening SiO with particle size of 0.4cm2As a ready-to-use granule. Preparing a standard chitosan acetic acid aqueous solution: weighing 1.5g of acetic acid and 1.2g of chitosan, adding distilled water for dissolving, transferring to a 1000mL volumetric flask, and fixing the volume to a scale mark by using distilled water to obtain the required standard solution. 35g of the silica is weighed out and dried at 80 ℃ for 2.5h, then the silica is put into 900mL of chitosan acetic acid aqueous solution, the solid is filtered out after 4.5h of oscillation at room temperature, and the solid is dried in vacuum. And (3) putting the dried solid into 0.3g/L glutaraldehyde water solution, oscillating for 2.5h, filtering out the solid, repeatedly washing and drying in vacuum to obtain the chitosan/silicon dioxide composite material, and repeating the steps to obtain a large number of immobilized carriers.
2. Immobilization of desulfurization strains
15g of the prepared chitosan/silicon dioxide composite material is put into 100mL of culture solution of desulfurization bacterial strain (thiobacillus thioparus with the registration number of CGMCC No.12756 in the preservation center and the Latin scientific name Thiobacillus) and the measured viable count is 1.35 × 109Shaking in a shaking table with the temperature of 28 ℃ and the rotating speed of 150r/min for 4h, filtering out the solid material, and putting the solid material into an incubator to carry out constant temperature culture for 6h at the temperature of 28 ℃ to obtain the immobilized desulfurization strain.
3. Biological desulfurization by immobilized desulfurization strain
Filling the prepared immobilized desulfurization strain into a biological trickling filter, wherein the filling volume ratio of the immobilized desulfurization strain is 12%, and then carrying out a biological desulfurization experiment, wherein the specific operation flow is as follows: 1.5% volSO2The raw material gas enters from the bottom of the absorption tower, the flow rate is 600L/h, the gas-liquid ratio is 25, and the raw material gas reacts with alkaline absorption liquid in the tower to generate SO3 2-/SO4 2-The solution and the alkaline absorption liquid mainly comprise the following components: KNO31.5g/L，NaHCO34g/L，MgCl20.3g/L，NH4Cl0.75g/L. The absorption liquid is treated by anaerobic reduction in the tower to obtain S2-A solution; s2-The solution flows in from the top of the biological trickling filter tower, and S passes through the immobilized desulfurization strain2-Absorbed by the desulfurization strains, metabolized to generate S elementary substance, precipitated, settled and filtered to obtain solid sulfur elementary substance. The whole device runs stably for 24h, and the temperature is controlled to be 27 ℃.
4. Processing the results
Through the analysis of purified gas and the analysis of S elementary substance products, the biological desulfurization efficiency of the scheme is about 86 percent, and the recovery rate of the S elementary substance is about 71 percent.
Example 3:
1. preparation of an immobilization carrier:
using macroporous resin as fixed template, burning and hydrolyzing silicon dioxide particles by silicate, and screening SiO with particle size of 0.5cm2As a ready-to-use granule. Preparing a standard chitosan acetic acid aqueous solution: weighing 2g of acetic acid and 1.5g of chitosan, adding distilled water for dissolving, transferring to a 1000mL volumetric flask, and fixing the volume to the scale mark by using distilled water to obtain the required standard solution. Weighing 50g of the silicon dioxide, drying at 80 ℃ for 3h, then putting the silicon dioxide into 1000mL of chitosan acetic acid aqueous solution, shaking at room temperature for 5h, filtering out solid, and drying in vacuum. And (3) putting the dried solid into 0.5g/L glutaraldehyde water solution, oscillating for 3h, filtering out the solid, repeatedly washing and drying in vacuum to obtain the chitosan/silicon dioxide composite material, and repeating the steps to obtain a large number of immobilized carriers.
2. Immobilization of desulfurization strains
20g of the prepared chitosan/silicon dioxide composite material is put into 100mL of culture solution of desulfurization strain (thiobacillus thioparus with the registration number of CGMCC No.12756 in the preservation center and the Latin scientific name Thiobacillus) and the viable count is 1.5 × 109Shaking in a shaking table at 30 deg.C and rotation speed of 180r/min for 5 hr, filtering out solid material, and culturing in an incubator at 30 deg.C for 8 hr to obtain immobilized desulfurization strain.
3. Biological desulfurization by immobilized desulfurization strain
Filling the prepared immobilized desulfurization strain into a biological trickling filter, and fixingThe filling volume ratio of the customized desulfurization strain is 15%, and then a biological desulfurization experiment is carried out, wherein the specific operation flow is as follows: 3% volSO2The raw material gas enters from the bottom of the anaerobic absorption tower, the flow rate is 1000L/min, the gas-liquid ratio is 30, and the raw material gas reacts with alkaline absorption liquid in the tower to generate SO3 2-/SO4 2-The solution and the alkaline absorption liquid mainly comprise the following components: KNO32g/L，NaHCO35g/L，MgCl20.5g/L，NH4Cl1 g/L. The absorption liquid is treated by anaerobic reduction in the tower to obtain S2-A solution; s2-The solution flows in from the top of the biological trickling filter tower, and S passes through the immobilized desulfurization strain2-Absorbed by the desulfurization strains, metabolized to generate S elementary substance, precipitated, settled and filtered to obtain solid sulfur elementary substance. The whole device runs stably for 24h, and the temperature is controlled to be 30 ℃.
4. Processing the results
Through analysis of purified gas and analysis of S elementary substance products, the biological desulfurization efficiency of the scheme is about 83%, and the recovery rate of the S elementary substance is about 82%.
Claims (9)
1. An immobilized desulfurization strain is characterized by being prepared by the following method, comprising the following specific steps:
A. preparation of immobilization support
Weighing dried silicon dioxide, and putting the silicon dioxide into a standard chitosan acetic acid aqueous solution, wherein the ratio of the silicon dioxide to the chitosan acetic acid aqueous solution is 20-50 g/L; after oscillation for 4-5 h, filtering out solids, and drying in vacuum; placing the dried solid material into a glutaraldehyde aqueous solution, oscillating for 2-3 h, filtering out the solid, repeatedly washing, and vacuum drying to obtain a chitosan-silicon dioxide composite material;
B. immobilization of desulfurization strains
And (2) putting the prepared chitosan-silicon dioxide composite material into a desulfurization strain culture solution, wherein the mass-volume ratio of the chitosan-silicon dioxide composite material to the desulfurization strain culture solution is 0.1-0.2 g/mL, oscillating the chitosan-silicon dioxide composite material in a shaker at the temperature of 25-30 ℃ and the rotating speed of 120-180 r/min for 3-5 h, filtering out a solid material, and putting the solid material into an incubator at the temperature of 25-30 ℃ for culturing for 5-8 h to obtain the immobilized desulfurization strain.
2. The immobilized desulfurization strain of claim 1, wherein the drying condition of the silica in step A is 80-90 ℃ and the drying time is 2-3 h.
3. The immobilized desulfurization strain of claim 1, wherein the silica particles in step A have a diameter of 0.3 to 0.5 cm; the solute components of the standard chitosan acetic acid aqueous solution are as follows: 1-2 g/L of acetic acid and 1-1.5 g/L of chitosan; the solute component of the glutaraldehyde aqueous solution is as follows: 0.1-0.5 g/L glutaraldehyde.
4. The immobilized desulfurization strain of claim 1, wherein the number of viable bacteria in the desulfurization strain culture solution in step B is 1.0 × 109～1.5×109one/mL.
5. A method for treating a gas containing sulfur dioxide by using the immobilized desulfurization strain of claim 1, comprising the following steps:
(1)SO2absorption and reduction of gas: SO (SO)2The raw material gas enters from the bottom of the anaerobic absorption tower and reacts with the circulating alkaline absorption liquid in the tower to generate SO3 2-/SO4 2-The solution and the absorption liquid are subjected to anaerobic reduction treatment in a tower to obtain S2-A solution;
(2)S2-oxidation and S recovery of the solution: s in step (1)2-The solution flows in from the top of the biotrickling filter packed with the immobilized desulfurization bacterial strain, S when passing through the immobilized desulfurization bacterial strain2-Absorbed by the desulfurization strains, biologically metabolized to generate sulfur simple substance, precipitated, settled and filtered to obtain solid sulfur simple substance.
6. The method according to claim 5, wherein SO is used in step (1)2In the raw material gas of (2)2The volume percentage content of the (B) is 0.1 to 3 percent.
7. The method according to claim 5, wherein the alkaline absorbent liquid in step (1) comprises: KNO31～2g/L，NaHCO33～5g/L，MgCl20.1～0.5g/L，NH4Cl 0.5～1g/L。
8. The method according to claim 5, wherein SO is used in step (1)2The flow of the gas entering the raw material gas is controlled to be 200-1000L/h; the gas-liquid ratio of the alkaline absorption liquid to the alkaline absorption liquid is controlled to be 15-30.
9. The method according to claim 5, characterized in that the packed volume percentage of immobilized desulfurization strains in the biotrickling filter is between 10% and 15%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010466081.XA CN111607586A (en) | 2020-05-28 | 2020-05-28 | Immobilized desulfurization strain and method for treating gas containing sulfur dioxide |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010466081.XA CN111607586A (en) | 2020-05-28 | 2020-05-28 | Immobilized desulfurization strain and method for treating gas containing sulfur dioxide |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN111607586A true CN111607586A (en) | 2020-09-01 |
Family
ID=72194535
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010466081.XA Withdrawn CN111607586A (en) | 2020-05-28 | 2020-05-28 | Immobilized desulfurization strain and method for treating gas containing sulfur dioxide |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN111607586A (en) |
Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1987002703A1 (en) * | 1985-10-22 | 1987-05-07 | National Research Development Corporation | Microcarrier for cell culture |
| JP2007038044A (en) * | 2005-07-29 | 2007-02-15 | Kurita Water Ind Ltd | Bio-desulfurization method and bio-desulfurization apparatus |
| CN101073745A (en) * | 2007-03-29 | 2007-11-21 | 南京大学 | Method for removing sulfur-dioxide in smoke by bamboo-carbon filler biological drip filtering tower |
| US20090258404A1 (en) * | 2006-06-30 | 2009-10-15 | Biogasol Ipr Aps | Production of fermentation products in biofilm reactors using microorganisms immobilised on sterilised granular sludge |
| US20140370578A1 (en) * | 2013-06-18 | 2014-12-18 | China Petroleum & Chemical Corporation | Modified ceramsite packing useful for biomembrane trickling filter and a process for removing so2 from flue gas using the trickling filter |
| CN104894099A (en) * | 2015-06-12 | 2015-09-09 | 福建省农业科学院中心实验室 | Bacteria immobilization particles for water purification and preparation method of bacteria immobilization particles |
| CN106310890A (en) * | 2015-06-17 | 2017-01-11 | 中国石油化工股份有限公司 | Method for removing acidic gases through biological technology |
| CN107217080A (en) * | 2017-07-31 | 2017-09-29 | 寰龙特种糖业有限公司 | A kind of method that utilization immobilised enzymes prepares jerusalem artichoke FOS |
| CN109207467A (en) * | 2018-11-19 | 2019-01-15 | 南京工业大学 | Cell immobilization method of thiobacillus thioparus |
| CN110449133A (en) * | 2019-07-16 | 2019-11-15 | 湖北工程学院 | Chitosan/silicon dioxide composite material |
-
2020
- 2020-05-28 CN CN202010466081.XA patent/CN111607586A/en not_active Withdrawn
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1987002703A1 (en) * | 1985-10-22 | 1987-05-07 | National Research Development Corporation | Microcarrier for cell culture |
| JP2007038044A (en) * | 2005-07-29 | 2007-02-15 | Kurita Water Ind Ltd | Bio-desulfurization method and bio-desulfurization apparatus |
| US20090258404A1 (en) * | 2006-06-30 | 2009-10-15 | Biogasol Ipr Aps | Production of fermentation products in biofilm reactors using microorganisms immobilised on sterilised granular sludge |
| CN101073745A (en) * | 2007-03-29 | 2007-11-21 | 南京大学 | Method for removing sulfur-dioxide in smoke by bamboo-carbon filler biological drip filtering tower |
| US20140370578A1 (en) * | 2013-06-18 | 2014-12-18 | China Petroleum & Chemical Corporation | Modified ceramsite packing useful for biomembrane trickling filter and a process for removing so2 from flue gas using the trickling filter |
| CN104894099A (en) * | 2015-06-12 | 2015-09-09 | 福建省农业科学院中心实验室 | Bacteria immobilization particles for water purification and preparation method of bacteria immobilization particles |
| CN106310890A (en) * | 2015-06-17 | 2017-01-11 | 中国石油化工股份有限公司 | Method for removing acidic gases through biological technology |
| CN107217080A (en) * | 2017-07-31 | 2017-09-29 | 寰龙特种糖业有限公司 | A kind of method that utilization immobilised enzymes prepares jerusalem artichoke FOS |
| CN109207467A (en) * | 2018-11-19 | 2019-01-15 | 南京工业大学 | Cell immobilization method of thiobacillus thioparus |
| CN110449133A (en) * | 2019-07-16 | 2019-11-15 | 湖北工程学院 | Chitosan/silicon dioxide composite material |
Non-Patent Citations (2)
| Title |
|---|
| 廖泽君等: "氧化亚铁硫杆菌固定化技术及其应用", 《金属矿山》 * |
| 王晓玲等: "固定化技术提高微生物对土壤中石油烃降解性能研究进展", 《安徽农业科学》 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN107537293B (en) | Closed cycle microbial desulfurization and elemental sulfur recovery method | |
| CN109095732B (en) | Process for preparing high-purity magnesium hydroxide based on magnesium-method desulfurization wastewater | |
| TW202012034A (en) | Acid gas treatment | |
| CN109621903B (en) | Method for preparing sludge-based carbon zeolite functional material | |
| CN1939840A (en) | Tail gas treatment and reutilization for calcium carbide stove | |
| CN113842883B (en) | Lanthanum-loaded iron carbon nanotube film material for environmental remediation and preparation method and application thereof | |
| CA2414871A1 (en) | Process and apparatus using a spray absorber bioreactor for the biocatalytic treatment of gases | |
| CN114751429B (en) | Treatment process for preparing baking soda from desulfurized fly ash | |
| CN109939549B (en) | Comprehensive treatment method and device for flue gas | |
| CN110643403A (en) | Chemical absorption combined biological removal hydrogen sulfide and sulfur resource technology in methane | |
| CN101480559B (en) | Method for recycling sulfureous in flue gas using film | |
| CN113769564B (en) | Semi-dry desulfurization ash solidified industrial flue gas carbon dioxide and recycling method thereof | |
| CN106746100B (en) | Treatment method of ethylene refining waste alkali liquor | |
| CN111607586A (en) | Immobilized desulfurization strain and method for treating gas containing sulfur dioxide | |
| CN109939560B (en) | Method and device for treating sulfur-containing flue gas | |
| CN114432869B (en) | Method and device for treating coal-fired flue gas | |
| CN103551137A (en) | Preparation method and application of solid catalyst using sludge-based activated carbon as matrix material | |
| CN111097273B (en) | Method and device for treating FCC (fluid catalytic cracking) regenerated flue gas | |
| CN108726541B (en) | Method for preparing sodium bisulfate by resource utilization of coal chemical industry waste gas and waste water | |
| CN102198363A (en) | Film based recovery method of sulfur in flue gas | |
| CN101481101B (en) | Method for recycling nitrogen from flue gas by using membrane | |
| CN114477653B (en) | Method and system for treating wastewater in molecular sieve production process | |
| CN102198362B (en) | Method for recovering nitrogen in flue gas with membrane | |
| CN115090101B (en) | Comprehensive treatment method for desulfurizing, dewatering and removing carbon dioxide of biogas | |
| CN115124062B (en) | Desulfurization gypsum recycling comprehensive utilization system and process |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20200901 |
|
| WW01 | Invention patent application withdrawn after publication |