CN111595840B - Preparation method and application of reagent permeation prevention paper-based array - Google Patents
Preparation method and application of reagent permeation prevention paper-based array Download PDFInfo
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Abstract
The invention discloses a preparation method and application of a reagent permeation prevention paper base array. The water-soluble reagent and the organic solvent can be effectively prevented from diffusing in the two arrays by the solidification and crosslinking of the anti-seepage reagent and the solidification reagent, so that the color of the paper base of the adjacent arrays is prevented from being covered in the colorimetric detection process, and the result misjudgment is caused. By loading one or more explosives detection reagents in the paper base of the array, multiple components of a complex explosive sample can be distinguished through one experiment, and the loaded reagents are localized, so that the effects of concentration, signal amplification and colorimetric reaction sensitivity improvement are achieved. The method has the advantages of simplicity, immediate result display, signal amplification, capability of detecting various explosive components at one time, short detection time and the like, and provides an effective technical means for on-site detection and analysis of the explosives.
Description
Technical Field
The invention belongs to the field of explosive detection and analysis, and particularly relates to a preparation method of a reagent permeation prevention paper-based array and application of the reagent permeation prevention paper-based array in explosive detection.
Background
At present, the common technologies or methods for detecting explosives on site mainly include portable raman technology, metal detection technology, ion mobility spectrometry technology, fluorescence spectrometry technology, and the like. The technologies generally have the problems of high cost, time consumption for detection, expensive instruments, need of professional detection technicians and the like, so that the development of a method for detecting explosives quickly, cheaply, accurately and in real time becomes a key point of research. Meanwhile, most of the current commercialized explosive detection equipment can only detect one explosive component at a time, when the equipment faces a complex explosive sample in an explosion field, the component can be determined by repeating a plurality of tests, similar analysis consumes time and labor, and the field application of the explosive detection reagent is greatly limited. The array assembly of various detection reagents is an effective way for realizing high-efficiency analysis of complex objects to be detected.
The matrix weave patterns of spun fibers proposed so far have rarely solved the problem of interpenetration between fibers in the matrix. The Won-Gun Koh group of subjects achieved the problem of preventing penetration between fibers by coating the fibers with a hydrogel and then selectively dissolving the uncoated fibers with a solvent (Advanced Functional Materials,2013,23, 591-597; Angew. chem.2015,127, 11673-11677).
The invention provides a preparation method of a reagent permeation prevention paper-based array and application thereof according to the technical problem of restricting on-site detection of explosives, the array can play a role of localization on a loaded reagent through loading with a detection reagent so as to generate unique concentration, signal amplification and permeation prevention functions, and can effectively prevent diffusion of a water-soluble reagent and an organic solvent in the two arrays, thereby preventing color coverage in the colorimetric detection process of adjacent arrays, causing misjudgment and finally improving the sensitivity of colorimetric reaction. Also, by loading several or more reagents in an array, multiple components of a complex explosive sample can be distinguished in one experiment.
Disclosure of Invention
The invention aims to provide a preparation method and application of a reagent permeation prevention paper-based array. The water-soluble reagent and the organic solvent can be effectively prevented from diffusing in the two arrays by the solidification and crosslinking of the anti-seepage reagent and the solidification reagent, so that the color of the paper base of the adjacent arrays is prevented from being covered in the colorimetric detection process, and the result misjudgment is caused. By loading one or more explosives detection reagents in the paper base of the array, multiple components of a complex explosive sample can be distinguished through one experiment, and the loaded reagents are localized, so that the effects of concentration, signal amplification and colorimetric reaction sensitivity improvement are achieved.
The preparation method of the reagent permeation prevention paper-based array comprises the following steps:
a. uniformly mixing a light curing agent and an anti-seepage agent in a light-shielding bottle according to the volume ratio of 100:1-1:100, magnetically stirring for 1-100min, then adding a photoinitiator with the volume fraction of 0.1-50%, and continuously stirring for 1-60min to obtain a mixed solution of an isolation layer (2), wherein the light curing agent is polymethyl methacrylate, hydroxyethyl methacrylate, ethyl 3-aminocrotonate, vigabatrin, 5-chloro-5-hexenoic acid, 2-methoxyethyl acrylate, polyethylene glycol diacrylate, polydimethylsiloxane or polystyrene; the seepage-proofing agent is 1H, 1H-perfluoropropyl methacrylate, 2,3,3, 3-pentafluoropropyl acrylate, 2,3,3, 3-pentafluoropropyl methacrylate, 2,3,3,4, 4-hexafluoro-1, 5-pentyl dimethacrylate, perfluoroallylbenzene, 1,1,1,3,3, 3-hexafluoroisopropyl methacrylate or 2,2,3, 3-tetrafluoropropyl methacrylate; the photoinitiator is: 2-hydroxy-2-methyl-1-phenyl-1-propanone, 2-benzyl-2-dimethylamino-1- (4-morpholinophenyl) butanone, triphenylsulfonium salt, dimethylformamide, 2-hydroxy-2-methyl-phenylacetone;
b. spreading a paper base (1) on a glass substrate, and fixing the periphery of the paper base material by using a fluorinated plate with the thickness of 100 mu m, wherein the paper base (1) is as follows: polyvinyl alcohol 1788, polyvinyl alcohol 1799, polyethylene oxide, chitosan, polyvinyl pyrrolidone, polyacrylic acid, polypropylene cellulose, polycarbonate, polyimide spun film, filter paper, nitrocellulose paper, rice paper, carbon paper, cellophane or cellulose paper;
c. dripping 1-1000 mu l of the mixed solution of the isolating layer (2) obtained in the step a onto the paper base (1) fixed in the step b, and covering a photomask plate on the paper base (1), wherein the size of an internal unit of the photomask plate is 100-500 mu m, and curing and crosslinking for 1-240s under the irradiation of an ultraviolet lamp with the wavelength of 254-390 nm;
d. and c, washing the cured paper base (1) obtained in the step c for 1-20min by using a developing solution which is deionized water, ethanol, acetone, tetrahydrofuran, ethylene glycol or dimethyl sulfoxide, developing, irradiating for 1-60min by using an ultraviolet lamp with 390nm of 254-.
The application of the reagent permeation prevention paper-based array obtained by the method in detecting explosives.
The explosive is standard and non-standard explosive raw materials, and comprises chlorate, permanganate, ammonium salt, nitrate, sulfur, urea and derivatives thereof, nitrogen-free explosive, dinitrotoluene, trinitrotoluene, p-nitrotoluene, trolene, picric acid, Taian explosive, hexogen or octogen, wherein the nitrogen-free explosive is TATP, DADP or HMTD.
The application of the reagent permeation prevention paper-based array in explosive detection is that 100 mu L of different types of explosive detection reagents are loaded in each area of the paper base of the array respectively, so that corresponding explosive particles or liquid can be directly detected; depending on the explosive detection reagent loaded, a variety of standard and non-standard explosive feedstocks can be detected and identified simultaneously, including chlorates, permanganates, ammonium salts, nitrates, sulfur, urea and its derivatives, nitrogen-free explosives (TATP, DADP, HMTD), dinitrotoluene, trinitrotoluene, paranitrotoluene, trolene, picric acid, Taian explosives, hexogen, or octogen.
According to the preparation method and the application of the reagent permeation prevention paper-based array, the permeation prevention paper-based array can play a localization role in the loaded reagent so as to generate unique concentration, signal amplification and permeation prevention effects. Also, by loading several or more reagents in the array, multiple components of a complex explosive sample can be distinguished in one operation. The method has the advantages of simple operation method, controllable size, sensitive reaction and immediate result display.
The invention has the advantages and beneficial effects that:
compared with the existing commercialized explosive identification technology, the preparation method of the reagent permeation prevention paper-based array prepared by the method can effectively prevent the diffusion of water-soluble reagents and organic solvents in the two arrays and prevent the permeation and coverage of colors in the detection process of adjacent arrays, thereby improving the sensitivity of colorimetric reaction. The method is simple, can display results and amplify signals when used for detecting explosives, can detect various explosive components at one time, greatly shortens the time of on-site detection of the explosives, and provides an effective technical means for on-site detection and analysis of the explosives.
Drawings
FIG. 1 is an optical photograph of an array of paper substrates of the present invention, wherein the paper substrate (1), a barrier layer (2);
FIG. 2 is a paper base array structure characterized by a scanning electron microscope, wherein a paper base (1) and an isolation layer (2) are adopted;
FIG. 3 is an optical photograph of a paper-based array of the present invention depicting the permeation prevention effect on the reagent before and after the permeation prevention reagent is added;
FIG. 4 is an optical photograph of a paper-based array according to the present invention, which is characterized in that the optical photograph can be used for the detection of explosives, and has the functions of displaying the result and amplifying the signal;
FIG. 5 is an optical photograph of a paper-based array of the present invention, which is characterized in that a plurality of explosive components can be detected at one time in the detection of explosives, thereby reducing the number of operations and shortening the time for analyzing the explosives in-situ detection.
Detailed Description
The present invention will be further illustrated by the following specific examples, but the present invention is not limited to these examples.
Example 1
a. Uniformly mixing a light curing agent polymethyl methacrylate and an anti-seepage agent 1H, 1H-perfluoropropyl methacrylate according to a volume ratio of 100:1 in a shading bottle, magnetically stirring for 100min, then adding a photoinitiator 2-hydroxy-2-methyl-1-phenyl-1-acetone with a volume fraction of 0.1%, and continuously stirring for 1min to obtain a mixed solution of an isolation layer 2;
b. flatly laying the paper base 1 on a glass substrate to be polyvinyl alcohol 1788, and fixing the paper base 1 to be around the polyvinyl alcohol 1788 by using a fluorinated plate with the thickness of 100 mu m;
c. dripping 1-1000 mul of the mixed solution of the isolation layer 2 obtained in the step a onto the fixed paper base 1 of the polyvinyl alcohol 1788 in the step b, covering a photomask plate with an inner unit size of 100 mu m above the paper base 1 of the polyvinyl alcohol 1788, and curing and crosslinking for 1s under the irradiation of a 254nm ultraviolet lamp;
d. and c, washing the cured paper base 1 obtained in the step c with polyvinyl alcohol 1788 and deionized water for 20min to wash away the separation layer mixed solution which is not cured, developing, irradiating for 60min with a 254nm ultraviolet lamp, and performing secondary curing and crosslinking to obtain the reagent permeation preventing paper base array.
Example 2
a. Uniformly mixing a light curing agent hydroxyethyl methacrylate and an anti-seepage agent 2,2,3,3, 3-pentafluoropropyl acrylate in a volume ratio of 1:100 in a light-shielding bottle, magnetically stirring for 1min, then adding a photoinitiator 2-benzyl-2-dimethylamino-1- (4-morpholinophenyl) butanone with the volume fraction of 50%, and continuously stirring for 60min to obtain a mixed solution of an isolation layer 2;
b. flatly laying paper base 1 polyvinyl alcohol 1799 on a glass substrate, and fixing the periphery of the paper base 1 polyvinyl alcohol 1799 by a fluorinated plate with the thickness of 100 mu m;
c. dripping 1000 mu l of the mixed solution of the isolation layer 2 obtained in the step a onto the paper base 1 polyvinyl alcohol 1799 fixed in the step b, covering a light mask plate with the internal unit size of 500 mu m above the paper base 1 polyvinyl alcohol 1799, and curing and crosslinking for 240s under the irradiation of a 390nm ultraviolet lamp;
d. and c, washing the cured paper base 1 polyvinyl alcohol 1799 obtained in the step c for 1min by using a developing solution which is ethanol, developing to wash away the mixed solution of the separation layers which are not cured, irradiating for 1min by using a 390nm ultraviolet lamp, and performing secondary curing and crosslinking to obtain the reagent permeation prevention paper base array.
Example 3
a. Uniformly mixing a light curing agent 3-amino ethyl crotonate and an anti-seepage agent 2,2,3,3, 3-pentafluoro propyl methacrylate in a shading bottle according to a volume ratio of 100:1, magnetically stirring for 50min, adding a photoinitiator triphenylsulfonium salt with a volume fraction of 25%, and continuously stirring for 30min to obtain a mixed solution of an isolation layer 2;
b. flatly paving paper base 1 polyoxyethylene on a glass substrate, and fixing the periphery of the paper base 1 polyoxyethylene material by using a fluorinated plate with the thickness of 100 mu m;
c. dripping 500 mu l of the mixed solution of the isolation layer 2 obtained in the step a onto the fixed paper base 1 polyoxyethylene in the step b, adding a cover photomask plate with the internal unit size of 250 mu m above the paper base 1 polyoxyethylene, and curing and crosslinking for 200s under the irradiation of a 365nm ultraviolet lamp;
d. and c, washing the cured paper base 1 polyethylene oxide obtained in the step c for 10min by using acetone as a developing solution, developing to wash away the separation layer mixed solution which is not cured, irradiating for 30min by using a 365nm ultraviolet lamp, and performing secondary curing and crosslinking to obtain the reagent permeation prevention paper base array.
Example 4
a. Uniformly mixing a light curing agent vigabatrin and an anti-seepage agent 2,2,3,3,4, 4-hexafluoro-1, 5-dimethyl amyl acrylate in a volume ratio of 100:1 in a shading bottle, magnetically stirring for 100min, then adding a photoinitiator dimethylformamide with the volume fraction of 0.1%, and continuously stirring for 1min to obtain a mixed solution of an isolation layer 2;
b. flatly paving paper base 1 chitosan on a glass substrate, and fixing the periphery of the paper base 1 chitosan material by using a fluorinated plate with the thickness of 100 mu m;
c. dripping 1 mu l of the mixed solution of the isolation layer 2 obtained in the step a onto the paper base 1 chitosan fixed in the step b, covering a light mask plate with the inner unit size of 100 mu m above the paper base 1 chitosan, and curing and crosslinking for 1s under the irradiation of a 254nm ultraviolet lamp;
d. and c, washing the cured paper base 1 chitosan obtained in the step c for 20min by using a developing solution which is tetrahydrofuran, developing to wash away the separation layer mixed solution which is not cured, irradiating for 60min by using a 254nm ultraviolet lamp, and performing secondary curing and crosslinking to obtain the reagent permeation prevention paper base array.
Example 5
a. Uniformly mixing a light curing agent 5-chloro-5-hexenoic acid and an anti-seepage agent perfluoroallyl benzene according to a volume ratio of 100:1 in a shading bottle, magnetically stirring for 100min, then adding a photoinitiator 2-hydroxy-2-methyl-phenyl acetone with a volume fraction of 0.1%, and continuously stirring for 1min to obtain a mixed solution of an isolation layer 2;
b. flatly laying paper base 1 polyvinylpyrrolidone on a glass substrate, and fixing the periphery of the paper base 1 polyvinylpyrrolidone material by using a fluorinated plate with the thickness of 100 mu m;
c. dripping 1 mu l of the mixed solution of the isolating layer 2 obtained in the step a onto the paper base 1 polyvinylpyrrolidone fixed in the step b, covering a light mask plate with the internal unit size of 100 mu m above the paper base 1 polyvinylpyrrolidone, and curing and crosslinking for 1s under the irradiation of a 254nm ultraviolet lamp;
d. and c, washing the cured paper base 1 obtained in the step c with ethylene glycol serving as a developing solution for 20min, developing to remove the non-cured isolation layer mixed solution, irradiating with a 254nm ultraviolet lamp for 60min, and performing secondary curing and crosslinking to obtain the reagent permeation preventing paper base array.
Example 6
a. Uniformly mixing a light curing agent 2-acrylic acid-2-methoxyethyl ester and an anti-seepage agent 1,1,1,3,3, 3-hexafluoroisopropyl methacrylate in a light-shielding bottle according to the volume ratio of 100:1, magnetically stirring for 100min, then adding a photoinitiator 2-hydroxy-2-methyl-1-phenyl-1-acetone with the volume fraction of 0.1%, and continuously stirring for 1min to obtain a mixed solution of an isolation layer 2;
b. flatly paving paper base 1 polypropylene cellulose on a glass substrate, and fixing the periphery of the paper base 1 polypropylene cellulose material by using a fluorinated plate with the thickness of 100 mu m;
c. dripping 1 mu l of the mixed solution of the isolating layer 2 obtained in the step a onto the fixed paper base 1 polypropylene cellulose in the step b, covering a light mask plate with the internal unit size of 100 mu m above the paper base 1 polypropylene cellulose, and curing and crosslinking for 60s under the irradiation of a 254nm ultraviolet lamp;
d. and c, washing the cured paper base 1 obtained in the step c for 20min by using a developing solution which is dimethyl sulfoxide, developing to wash away the separation layer mixed solution which is not cured, irradiating for 60min by using a 254nm ultraviolet lamp, and performing secondary curing and crosslinking to obtain the reagent permeation prevention paper base array.
Example 7
a. Uniformly mixing a light curing agent polyethylene glycol diacrylate and an anti-seepage agent 2,2,3, 3-tetrafluoropropyl methacrylate in a volume ratio of 100:1 in a shading bottle, magnetically stirring for 100min, then adding a photoinitiator 2-benzyl-2-dimethylamino-1- (4-morpholinylphenyl) butanone with the volume fraction of 0.1%, and continuously stirring for 1min to obtain a mixed solution of an isolation layer 2;
b. flatly laying paper base 1 polycarbonate on a glass substrate, and fixing the periphery of the paper base 1 polycarbonate material by using a fluorinated plate with the thickness of 100 mu m;
c. dripping 1 mu l of the mixed solution of the isolating layer 2 obtained in the step a onto the paper base 1 polycarbonate fixed in the step b, covering a light mask plate with the internal unit size of 100 mu m above the paper base 1 polycarbonate, and curing and crosslinking for 1s under the irradiation of an ultraviolet lamp with the wavelength of 254 nm;
d. and c, washing the cured paper base 1 polycarbonate obtained in the step c for 20min by using a developing solution which is dimethyl sulfoxide, developing to wash away the separation layer mixed solution which is not cured, irradiating for 60min by using a 254nm ultraviolet lamp, and performing secondary curing and crosslinking to obtain the reagent permeation prevention paper base array.
Example 8
a. Uniformly mixing a light curing agent polydimethylsiloxane and an anti-seepage agent 1H, 1H-perfluoropropyl methacrylate in a shading bottle according to the volume ratio of 1:100, magnetically stirring for 1min, then adding a photoinitiator triphenylsulfur salt with the volume fraction of 50%, and continuously stirring for 60min to obtain a mixed solution of an isolation layer 2;
b. flatly paving a paper base 1 polyimide spinning membrane on a glass substrate, and fixing the periphery of the paper base 1 polyimide spinning membrane material by using a fluorinated plate with the thickness of 100 mu m;
c. dripping 1000 mu l of the mixed solution of the isolating layer 2 obtained in the step a onto the fixed polyimide spinning film of the paper base 1 in the step b, covering a light mask plate with the internal unit size of 500 mu m above the polyimide spinning film of the paper base 1, and curing and crosslinking for 240s under the irradiation of a 390nm ultraviolet lamp;
d. and c, washing the cured paper base 1 polyimide spinning film obtained in the step c for 1min by using a developing solution as deionized water, developing to wash away the separation layer mixed solution which is not cured, irradiating for 1min by using a 390nm ultraviolet lamp, and performing secondary curing and crosslinking to obtain the reagent permeation prevention paper base array.
Example 9
a. Uniformly mixing a light curing agent polystyrene and an anti-seepage agent 2,2,3,3, 3-pentafluoropropyl acrylate in a shading bottle according to a volume ratio of 1:100, magnetically stirring for 1min, then adding a photoinitiator dimethylformamide with a volume fraction of 50%, and continuously stirring for 60min to obtain a mixed solution of an isolation layer 2;
b. flatly paving paper base 1 filter paper on a glass substrate, and fixing the periphery of the paper base 1 filter paper material by using a fluorinated plate with the thickness of 100 mu m;
c. dripping 1000 mu l of the mixed solution of the isolating layer 2 obtained in the step a onto the paper base 1 fixed in the step b, covering a light mask plate with the internal unit size of 500 mu m above the paper base 1, and curing and crosslinking for 240s under the irradiation of a 390nm ultraviolet lamp;
d. and c, washing the cured paper base 1 filter paper obtained in the step c for 5min by using a developing solution as ethanol, developing to wash away the non-cured isolation layer mixed solution, irradiating for 10min by using a 390nm ultraviolet lamp, and performing secondary curing and crosslinking to obtain the reagent permeation prevention paper base array.
Example 10
a. Uniformly mixing a light curing agent polymethyl methacrylate and an anti-seepage agent 2,2,3,3, 3-pentafluoro propyl methacrylate in a shading bottle according to the volume ratio of 1:100, magnetically stirring for 10min, then adding a photoinitiator 2-hydroxy-2-methyl-phenyl acetone with the volume fraction of 50%, and continuously stirring for 10min to obtain a mixed solution of an isolation layer 2;
b. spreading the paper base 1 nitrocellulose paper on a glass substrate, and fixing the periphery of the paper base 1 nitrocellulose paper material by using a fluorinated plate with the thickness of 100 mu m;
c. dripping 100 mu l of the mixed solution of the isolation layer 2 obtained in the step a onto the paper base 1 nitrocellulose paper fixed in the step b, covering a photomask plate with the internal unit size of 200 mu m above the paper base 1 nitrocellulose paper, and curing and crosslinking for 100s under the irradiation of a 254nm ultraviolet lamp;
d. and c, washing the cured paper base 1 nitrocellulose paper obtained in the step c for 10min by using acetone as a developing solution, developing to wash away the non-cured isolation layer mixed solution, irradiating for 10min by using a 254nm ultraviolet lamp, and performing secondary curing and crosslinking to obtain the reagent permeation preventing paper base array.
Example 11
a. Uniformly mixing a light curing agent hydroxyethyl methacrylate and an anti-seepage agent 2,2,3,3,4, 4-hexafluoro-1, 5-dimethyl amyl acrylate in a volume ratio of 1:100 in a shading bottle, magnetically stirring for 15min, then adding a photoinitiator 2-hydroxy-2-methyl-1-phenyl-1-acetone with the volume fraction of 10%, and continuously stirring for 20min to obtain a mixed solution of an isolation layer 2;
b. flatly paving paper base 1 rice paper on a glass substrate, and fixing the periphery of the paper base 1 rice paper material by using a fluorinated plate with the thickness of 100 mu m;
c. dripping 500 mu l of the mixed solution of the isolating layer 2 obtained in the step a onto the paper base 1 fixed in the step b, covering a light mask plate with the internal unit size of 200 mu m above the paper base 1, and curing and crosslinking for 150s under the irradiation of a 390nm ultraviolet lamp;
d. and c, washing the cured paper base 1 rice paper obtained in the step c for 15min by using tetrahydrofuran as a developing solution, developing to wash away the separation layer mixed solution which is not cured, irradiating for 30min by using a 390nm ultraviolet lamp, and performing secondary curing and crosslinking to obtain the reagent permeation prevention paper base array.
Example 12
a. Uniformly mixing a light curing agent ethyl 3-aminocrotonate and an anti-seepage agent perfluoroallyl benzene according to a volume ratio of 1-1:100 in a shading bottle, magnetically stirring for 40min, then adding a photoinitiator 2-benzyl-2-dimethylamino-1- (4-morpholinyl phenyl) butanone with the volume fraction of 20%, and continuously stirring for 25min to obtain a mixed solution of an isolation layer 2;
b. spreading paper base 1 carbon paper on a glass substrate, and fixing the periphery of the paper base 1 carbon paper material by a fluorinated plate with the thickness of 100 mu m;
c. dripping 200 mu l of the mixed solution of the isolating layer 2 obtained in the step a onto the paper base 1 copy paper which is fixed in the step b, covering a light mask plate with the internal unit size of 300 mu m on the paper base 1 copy paper, and curing and crosslinking for 150s under the irradiation of a 254nm ultraviolet lamp;
d. and c, washing the cured paper base 1 carbon paper obtained in the step c for 12min by using tetrahydrofuran as a developing solution, developing to wash away the non-cured isolation layer mixed solution, irradiating for 40min by using a 254nm ultraviolet lamp, and performing secondary curing and crosslinking to obtain the reagent permeation preventing paper base array.
Example 13
a. Uniformly mixing a light curing agent vigabate acid and an anti-seepage agent 1,1,1,3,3, 3-hexafluoroisopropyl methacrylate in a light-shielding bottle according to the volume ratio of 1:100, magnetically stirring for 80min, then adding a photoinitiator triphenylsulfur salt with the volume fraction of 40%, and continuously stirring for 40min to obtain a mixed solution of an isolation layer 2;
b. flatly paving paper base 1 glass paper on a glass substrate, and fixing the periphery of the paper base 1 glass paper material by using a fluorinated plate with the thickness of 100 mu m;
c. b, dripping 600 mu l of the mixed solution of the isolating layer 2 obtained in the step a onto the paper base 1 glass paper fixed in the step b, covering a light mask plate with the internal unit size of 400 mu m above the paper base 1 glass paper, and curing and crosslinking for 50s under the irradiation of a 390nm ultraviolet lamp;
d. and c, washing the cured paper base 1 glass paper obtained in the step c for 18min by using a developing solution which is ethylene glycol, developing to wash away the separation layer mixed solution which is not cured, irradiating for 50min by using a 390nm ultraviolet lamp, and performing secondary curing and crosslinking to obtain the reagent permeation prevention paper base array.
Example 14
a. Uniformly mixing a light curing agent 5-chloro-5-hexenoic acid and an anti-seepage agent 2,2,3, 3-tetrafluoropropyl methacrylate in a shading bottle according to the volume ratio of 1:100, magnetically stirring for 70min, then adding a photoinitiator dimethylformamide with the volume fraction of 35%, and continuously stirring for 50min to obtain a mixed solution of an isolation layer 2;
b. flatly laying paper base 1 cellulose paper on a glass substrate, and fixing the periphery of the paper base 1 cellulose paper material by using a fluorinated plate with the thickness of 100 mu m;
c. dripping 80 mu l of the mixed solution of the isolating layer 2 obtained in the step a onto the paper base 1 cellulose paper which is fixed in the step b, covering a light mask plate with the internal unit size of 250 mu m above the paper base 1 cellulose paper, and curing and crosslinking for 230s under the irradiation of a 254nm ultraviolet lamp;
d. and c, washing the cured paper base 1 cellulose paper obtained in the step c for 8min by using dimethyl sulfoxide as a developing solution, developing to wash away the separation layer mixed solution which is not cured, irradiating for 55min by using a 254nm ultraviolet lamp, and performing secondary curing and crosslinking to obtain the reagent permeation prevention paper base array.
Example 15
a. Uniformly mixing 2-acrylic acid-2-methoxyethyl acrylate serving as a light curing agent and 1H, 1H-perfluoropropyl methacrylate serving as an anti-seepage agent in a shading bottle according to a volume ratio of 50:1, magnetically stirring for 55min, then adding 25% by volume of 2-hydroxy-2-methyl-phenyl acetone serving as a photoinitiator, and continuously stirring for 55min to obtain a mixed solution of an isolation layer 2;
b. flatly laying paper base 1 polyvinyl alcohol 1799 on a glass substrate, and fixing the periphery of the paper base polyvinyl alcohol 1799 material by a fluorinated plate with the thickness of 100 mu m;
c. dripping 1-1000 mul of the mixed solution of the isolating layer 2 obtained in the step a onto the fixed paper base 1 polyvinyl alcohol 1799 in the step b, covering a light mask plate with the internal unit size of 500 mu m above the paper base 1 polyvinyl alcohol 1799, and curing and crosslinking for 180s under the irradiation of a 390nm ultraviolet lamp;
d. and c, washing the cured paper base 1 polyvinyl alcohol 1799 obtained in the step c for 20min by using a developing solution dimethyl sulfoxide, developing, irradiating for 60min by using a 390nm ultraviolet lamp, and performing secondary curing and crosslinking to obtain the reagent permeation prevention paper base array.
Example 16
a. Uniformly mixing a light curing agent polyethylene glycol diacrylate and an anti-seepage agent 2,2,3,3, 3-pentafluoropropyl acrylate in a volume ratio of 1:100 in a shading bottle, magnetically stirring for 20min, then adding a photoinitiator 2-hydroxy-2-methyl-1-phenyl-1-acetone with a volume fraction of 25%, and continuously stirring for 30min to obtain a mixed solution of an isolation layer 2;
b. flatly paving paper base 1 chitosan on a glass substrate, and fixing the periphery of the paper base 1 chitosan material by using a fluorinated plate with the thickness of 100 mu m;
c. dripping 500 mu l of the mixed solution of the isolating layer 2 obtained in the step a onto the paper base 1 chitosan fixed in the step b, covering a light mask plate with the internal unit size of 250 mu m above the paper base 1 chitosan, and curing and crosslinking for 200s under the irradiation of a 390nm ultraviolet lamp;
d. and c, washing the cured paper base 1 chitosan obtained in the step c for 10min by using a developing solution as deionized water, developing to wash away the separation layer mixed solution which is not cured, irradiating for 30min by using a 390nm ultraviolet lamp, and performing secondary curing and crosslinking to obtain the reagent permeation prevention paper base array.
Example 17
The reagent permeation prevention paper-based arrays prepared in examples 1-16 were applied to the detection of explosives:
respectively loading 100ml of chlorate, permanganate, ammonium salt, nitrate, sulfur, urea and derivatives thereof, and several or more detection reagents of nitrogen-free explosives including TATP, DADP or HMTD, dinitrotoluene, trinitrotoluene, p-nitrotoluene, troxer, picric acid, Taian explosive, hexogen or octogen explosives in a paper-based area of the reagent-permeation-proof paper-based array to obtain an anti-permeation paper-based detection array substrate loaded with the explosive detection reagent, and directly using the anti-permeation paper-based detection array loaded with the explosive detection reagent to detect corresponding types of explosive particles or liquid.
Claims (1)
1. The application of a reagent permeation prevention paper-based array in detecting explosives is characterized in that the preparation of the reagent permeation prevention paper-based array is carried out according to the following steps:
a. uniformly mixing a light curing agent and an anti-seepage agent in a light-shielding bottle according to the volume ratio of 100:1-1:100, magnetically stirring for 1-100min, then adding a photoinitiator with the volume fraction of 0.1-50%, and continuously stirring for 1-60min to obtain a mixed solution of an isolation layer (2), wherein the light curing agent is polymethyl methacrylate, hydroxyethyl methacrylate, ethyl 3-aminocrotonate, vigabatrin, 5-chloro-5-hexenoic acid, 2-acrylic acid-2-methoxyethyl ester, polyethylene glycol diacrylate, polydimethylsiloxane or polystyrene; the seepage-proofing agent is 1H, 1H-perfluoropropyl methacrylate, 2,3,3, 3-pentafluoropropyl acrylate, 2,3,3, 3-pentafluoropropyl methacrylate, 2,3,3,4, 4-hexafluoro-1, 5-pentyl dimethacrylate, perfluoroallylbenzene, 1,1,1,3,3, 3-hexafluoroisopropyl methacrylate or 2,2,3, 3-tetrafluoropropyl methacrylate; the photoinitiator is: 2-hydroxy-2-methyl-1-phenyl-1-propanone, 2-benzyl-2-dimethylamino-1- (4-morpholinophenyl) butanone, triphenylsulfonium salt, dimethylformamide, 2-hydroxy-2-methyl-phenylacetone;
b. spreading a paper base (1) on a glass substrate, and fixing the periphery of the paper base material by using a fluorinated plate with the thickness of 100 mu m, wherein the paper base (1) is as follows: polyvinyl alcohol 1788, polyvinyl alcohol 1799, polyethylene oxide, chitosan, polyvinyl pyrrolidone, polyacrylic acid, polypropylene cellulose, polycarbonate, polyimide spun film, filter paper, nitrocellulose paper, rice paper, carbon paper, cellophane or cellulose paper;
c. dripping 1-1000 mu l of the mixed solution of the isolating layer (2) obtained in the step a onto the paper base (1) fixed in the step b, and covering a photomask plate on the paper base (1), wherein the size of an internal unit of the photomask plate is 100-500 mu m, and curing and crosslinking for 1-240s under the irradiation of an ultraviolet lamp with the wavelength of 254-390 nm;
d. washing the cured paper base (1) obtained in the step c for 1-20min by using a developing solution which is deionized water, ethanol, acetone, tetrahydrofuran, ethylene glycol or dimethyl sulfoxide, developing, and irradiating for 1-60min by using an ultraviolet lamp with the wavelength of 254-;
detection of explosives:
e. the obtained reagent permeation prevention paper base array is loaded with several or more detection reagents of 100ml of chlorate, permanganate, ammonium salt, nitrate, sulfur, urea and derivatives thereof, TATP, DADP, HMTD, dinitrotoluene, trinitrotoluene, p-nitrotoluene, troxer, picric acid, Taian explosive, hexogen or octogen explosives in the area of a paper base (1) respectively to obtain an anti-permeation paper base detection array substrate loaded with the explosive detection reagent, and then the anti-permeation paper base detection array loaded with the explosive detection reagent is directly used for detecting corresponding types of explosive particles or liquid.
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| CN104677896A (en) * | 2015-01-31 | 2015-06-03 | 太原理工大学 | Preparation and application of paper-based microfluidic chip for colorimetric analysis |
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