CN111568781A - 一种护肤凝胶及其制备方法 - Google Patents
一种护肤凝胶及其制备方法 Download PDFInfo
- Publication number
- CN111568781A CN111568781A CN202010599999.1A CN202010599999A CN111568781A CN 111568781 A CN111568781 A CN 111568781A CN 202010599999 A CN202010599999 A CN 202010599999A CN 111568781 A CN111568781 A CN 111568781A
- Authority
- CN
- China
- Prior art keywords
- parts
- extract
- growth hormone
- human growth
- skin
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000002360 preparation method Methods 0.000 title abstract description 12
- 238000001879 gelation Methods 0.000 title description 2
- 239000000284 extract Substances 0.000 claims abstract description 61
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims abstract description 38
- 102000002265 Human Growth Hormone Human genes 0.000 claims abstract description 32
- 108010000521 Human Growth Hormone Proteins 0.000 claims abstract description 32
- 239000000854 Human Growth Hormone Substances 0.000 claims abstract description 32
- TXFPEBPIARQUIG-UHFFFAOYSA-N 4'-hydroxyacetophenone Chemical compound CC(=O)C1=CC=C(O)C=C1 TXFPEBPIARQUIG-UHFFFAOYSA-N 0.000 claims abstract description 28
- 229920002385 Sodium hyaluronate Polymers 0.000 claims abstract description 17
- 229940010747 sodium hyaluronate Drugs 0.000 claims abstract description 17
- YWIVKILSMZOHHF-QJZPQSOGSA-N sodium;(2s,3s,4s,5r,6r)-6-[(2s,3r,4r,5s,6r)-3-acetamido-2-[(2s,3s,4r,5r,6r)-6-[(2r,3r,4r,5s,6r)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2- Chemical compound [Na+].CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 YWIVKILSMZOHHF-QJZPQSOGSA-N 0.000 claims abstract description 17
- NIXOWILDQLNWCW-UHFFFAOYSA-N Acrylic acid Chemical compound OC(=O)C=C NIXOWILDQLNWCW-UHFFFAOYSA-N 0.000 claims abstract description 16
- 229920002125 Sokalan® Polymers 0.000 claims abstract description 16
- 229960001631 carbomer Drugs 0.000 claims abstract description 16
- 229920001184 polypeptide Polymers 0.000 claims abstract description 16
- 102000004196 processed proteins & peptides Human genes 0.000 claims abstract description 16
- 244000068988 Glycine max Species 0.000 claims abstract description 15
- 235000010469 Glycine max Nutrition 0.000 claims abstract description 15
- FYGDTMLNYKFZSV-URKRLVJHSA-N (2s,3r,4s,5s,6r)-2-[(2r,4r,5r,6s)-4,5-dihydroxy-2-(hydroxymethyl)-6-[(2r,4r,5r,6s)-4,5,6-trihydroxy-2-(hydroxymethyl)oxan-3-yl]oxyoxan-3-yl]oxy-6-(hydroxymethyl)oxane-3,4,5-triol Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC1[C@@H](CO)O[C@@H](OC2[C@H](O[C@H](O)[C@H](O)[C@H]2O)CO)[C@H](O)[C@H]1O FYGDTMLNYKFZSV-URKRLVJHSA-N 0.000 claims abstract description 14
- 229940015975 1,2-hexanediol Drugs 0.000 claims abstract description 14
- 229920002498 Beta-glucan Polymers 0.000 claims abstract description 14
- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 claims abstract description 14
- 229920000663 Hydroxyethyl cellulose Polymers 0.000 claims abstract description 14
- 239000004354 Hydroxyethyl cellulose Substances 0.000 claims abstract description 14
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 claims abstract description 14
- GSEJCLTVZPLZKY-UHFFFAOYSA-N Triethanolamine Chemical compound OCCN(CCO)CCO GSEJCLTVZPLZKY-UHFFFAOYSA-N 0.000 claims abstract description 14
- 239000004220 glutamic acid Substances 0.000 claims abstract description 14
- 235000013922 glutamic acid Nutrition 0.000 claims abstract description 14
- FHKSXSQHXQEMOK-UHFFFAOYSA-N hexane-1,2-diol Chemical compound CCCCC(O)CO FHKSXSQHXQEMOK-UHFFFAOYSA-N 0.000 claims abstract description 14
- 235000019447 hydroxyethyl cellulose Nutrition 0.000 claims abstract description 14
- 229930182817 methionine Natural products 0.000 claims abstract description 14
- 244000042430 Rhodiola rosea Species 0.000 claims abstract description 13
- 235000003713 Rhodiola rosea Nutrition 0.000 claims abstract description 13
- 238000002156 mixing Methods 0.000 claims abstract description 12
- 235000003145 Hippophae rhamnoides Nutrition 0.000 claims abstract description 10
- 235000013399 edible fruits Nutrition 0.000 claims abstract description 10
- 229940084750 sophora flavescens root extract Drugs 0.000 claims abstract description 10
- WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 claims abstract description 6
- 229940071826 hydroxyethyl cellulose Drugs 0.000 claims abstract description 6
- 239000002994 raw material Substances 0.000 claims abstract description 6
- 239000009429 Ginkgo biloba extract Substances 0.000 claims abstract description 5
- 235000020686 ginkgo biloba extract Nutrition 0.000 claims abstract description 5
- 229940068052 ginkgo biloba extract Drugs 0.000 claims abstract description 5
- 241000235342 Saccharomycetes Species 0.000 claims abstract description 4
- 240000000950 Hippophae rhamnoides Species 0.000 claims abstract 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 21
- 239000000203 mixture Substances 0.000 claims description 20
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 15
- 239000002502 liposome Substances 0.000 claims description 13
- 235000011201 Ginkgo Nutrition 0.000 claims description 11
- 235000008100 Ginkgo biloba Nutrition 0.000 claims description 11
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 11
- 238000006243 chemical reaction Methods 0.000 claims description 11
- 238000001816 cooling Methods 0.000 claims description 11
- 238000010438 heat treatment Methods 0.000 claims description 10
- 238000003756 stirring Methods 0.000 claims description 10
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 claims description 7
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 claims description 7
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 claims description 7
- 238000000034 method Methods 0.000 claims description 5
- 239000000546 pharmaceutical excipient Substances 0.000 claims description 4
- 238000005538 encapsulation Methods 0.000 claims description 3
- 238000005516 engineering process Methods 0.000 claims description 3
- 239000000843 powder Substances 0.000 claims description 3
- 244000194101 Ginkgo biloba Species 0.000 claims 2
- 210000004027 cell Anatomy 0.000 abstract description 22
- 102000008186 Collagen Human genes 0.000 abstract description 16
- 108010035532 Collagen Proteins 0.000 abstract description 16
- 229920001436 collagen Polymers 0.000 abstract description 16
- 210000002950 fibroblast Anatomy 0.000 abstract description 7
- 230000001737 promoting effect Effects 0.000 abstract description 4
- 102000012422 Collagen Type I Human genes 0.000 abstract description 3
- 108010022452 Collagen Type I Proteins 0.000 abstract description 3
- 238000010521 absorption reaction Methods 0.000 abstract description 3
- 230000009286 beneficial effect Effects 0.000 abstract description 3
- 230000004071 biological effect Effects 0.000 abstract description 3
- 230000015556 catabolic process Effects 0.000 abstract description 3
- 230000005779 cell damage Effects 0.000 abstract description 3
- 208000037887 cell injury Diseases 0.000 abstract description 3
- 238000006731 degradation reaction Methods 0.000 abstract description 3
- 238000005502 peroxidation Methods 0.000 abstract description 3
- 230000037394 skin elasticity Effects 0.000 abstract description 3
- 230000002708 enhancing effect Effects 0.000 abstract description 2
- 231100000957 no side effect Toxicity 0.000 abstract description 2
- 239000000126 substance Substances 0.000 abstract description 2
- 108010038988 Peptide Hormones Proteins 0.000 abstract 1
- 239000000813 peptide hormone Substances 0.000 abstract 1
- 210000003491 skin Anatomy 0.000 description 48
- 239000000499 gel Substances 0.000 description 21
- 230000000694 effects Effects 0.000 description 20
- 238000002474 experimental method Methods 0.000 description 12
- 241000229143 Hippophae Species 0.000 description 11
- 235000011187 glycerol Nutrition 0.000 description 10
- 241000218628 Ginkgo Species 0.000 description 9
- PMMYEEVYMWASQN-DMTCNVIQSA-N Hydroxyproline Chemical compound O[C@H]1CN[C@H](C(O)=O)C1 PMMYEEVYMWASQN-DMTCNVIQSA-N 0.000 description 9
- 239000000243 solution Substances 0.000 description 9
- 210000000434 stratum corneum Anatomy 0.000 description 9
- 239000000047 product Substances 0.000 description 8
- 230000000052 comparative effect Effects 0.000 description 7
- 238000001514 detection method Methods 0.000 description 7
- 238000012360 testing method Methods 0.000 description 7
- PMMYEEVYMWASQN-UHFFFAOYSA-N dl-hydroxyproline Natural products OC1C[NH2+]C(C([O-])=O)C1 PMMYEEVYMWASQN-UHFFFAOYSA-N 0.000 description 6
- 229960002591 hydroxyproline Drugs 0.000 description 6
- FGMPLJWBKKVCDB-UHFFFAOYSA-N trans-L-hydroxy-proline Natural products ON1CCCC1C(O)=O FGMPLJWBKKVCDB-UHFFFAOYSA-N 0.000 description 6
- 235000003935 Hippophae Nutrition 0.000 description 5
- 238000012258 culturing Methods 0.000 description 5
- 230000006870 function Effects 0.000 description 5
- 230000003020 moisturizing effect Effects 0.000 description 5
- 230000037303 wrinkles Effects 0.000 description 5
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 4
- XUMBMVFBXHLACL-UHFFFAOYSA-N Melanin Chemical compound O=C1C(=O)C(C2=CNC3=C(C(C(=O)C4=C32)=O)C)=C2C4=CNC2=C1C XUMBMVFBXHLACL-UHFFFAOYSA-N 0.000 description 4
- 230000015572 biosynthetic process Effects 0.000 description 4
- 229910002092 carbon dioxide Inorganic materials 0.000 description 4
- 230000008859 change Effects 0.000 description 4
- 239000002537 cosmetic Substances 0.000 description 4
- 230000012010 growth Effects 0.000 description 4
- 235000018102 proteins Nutrition 0.000 description 4
- 102000004169 proteins and genes Human genes 0.000 description 4
- 108090000623 proteins and genes Proteins 0.000 description 4
- 239000003381 stabilizer Substances 0.000 description 4
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 3
- 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 description 3
- GAMYVSCDDLXAQW-AOIWZFSPSA-N Thermopsosid Natural products O(C)c1c(O)ccc(C=2Oc3c(c(O)cc(O[C@H]4[C@H](O)[C@@H](O)[C@H](O)[C@H](CO)O4)c3)C(=O)C=2)c1 GAMYVSCDDLXAQW-AOIWZFSPSA-N 0.000 description 3
- 230000003712 anti-aging effect Effects 0.000 description 3
- 239000003153 chemical reaction reagent Substances 0.000 description 3
- 230000007423 decrease Effects 0.000 description 3
- 230000003247 decreasing effect Effects 0.000 description 3
- 239000000835 fiber Substances 0.000 description 3
- 229930003944 flavone Natural products 0.000 description 3
- 150000002212 flavone derivatives Chemical class 0.000 description 3
- 235000011949 flavones Nutrition 0.000 description 3
- 239000002609 medium Substances 0.000 description 3
- 150000003254 radicals Chemical class 0.000 description 3
- 230000028327 secretion Effects 0.000 description 3
- 210000000582 semen Anatomy 0.000 description 3
- 210000004927 skin cell Anatomy 0.000 description 3
- 210000001626 skin fibroblast Anatomy 0.000 description 3
- 239000006228 supernatant Substances 0.000 description 3
- VHBFFQKBGNRLFZ-UHFFFAOYSA-N vitamin p Natural products O1C2=CC=CC=C2C(=O)C=C1C1=CC=CC=C1 VHBFFQKBGNRLFZ-UHFFFAOYSA-N 0.000 description 3
- GVJHHUAWPYXKBD-UHFFFAOYSA-N (±)-α-Tocopherol Chemical compound OC1=C(C)C(C)=C2OC(CCCC(C)CCCC(C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-UHFFFAOYSA-N 0.000 description 2
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 2
- 241000246044 Sophora flavescens Species 0.000 description 2
- 102000003425 Tyrosinase Human genes 0.000 description 2
- 108060008724 Tyrosinase Proteins 0.000 description 2
- 230000009471 action Effects 0.000 description 2
- 239000013543 active substance Substances 0.000 description 2
- 230000032683 aging Effects 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- 230000004888 barrier function Effects 0.000 description 2
- 239000001569 carbon dioxide Substances 0.000 description 2
- 230000010261 cell growth Effects 0.000 description 2
- 239000006285 cell suspension Substances 0.000 description 2
- 239000012228 culture supernatant Substances 0.000 description 2
- 210000004207 dermis Anatomy 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 230000007062 hydrolysis Effects 0.000 description 2
- 238000006460 hydrolysis reaction Methods 0.000 description 2
- 150000002632 lipids Chemical class 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 239000012466 permeate Substances 0.000 description 2
- 239000008055 phosphate buffer solution Substances 0.000 description 2
- 239000000049 pigment Substances 0.000 description 2
- 229920000642 polymer Polymers 0.000 description 2
- 230000035755 proliferation Effects 0.000 description 2
- 238000003786 synthesis reaction Methods 0.000 description 2
- 210000001519 tissue Anatomy 0.000 description 2
- 231100000331 toxic Toxicity 0.000 description 2
- 230000002588 toxic effect Effects 0.000 description 2
- 230000002087 whitening effect Effects 0.000 description 2
- KIUKXJAPPMFGSW-DNGZLQJQSA-N (2S,3S,4S,5R,6R)-6-[(2S,3R,4R,5S,6R)-3-Acetamido-2-[(2S,3S,4R,5R,6R)-6-[(2R,3R,4R,5S,6R)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylic acid Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 KIUKXJAPPMFGSW-DNGZLQJQSA-N 0.000 description 1
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 102000029816 Collagenase Human genes 0.000 description 1
- 108060005980 Collagenase Proteins 0.000 description 1
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 description 1
- 206010013786 Dry skin Diseases 0.000 description 1
- 208000002197 Ehlers-Danlos syndrome Diseases 0.000 description 1
- 102000016942 Elastin Human genes 0.000 description 1
- 108010014258 Elastin Proteins 0.000 description 1
- 102000010834 Extracellular Matrix Proteins Human genes 0.000 description 1
- 108010037362 Extracellular Matrix Proteins Proteins 0.000 description 1
- 102000003886 Glycoproteins Human genes 0.000 description 1
- 108090000288 Glycoproteins Proteins 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- ZOKXTWBITQBERF-UHFFFAOYSA-N Molybdenum Chemical compound [Mo] ZOKXTWBITQBERF-UHFFFAOYSA-N 0.000 description 1
- 208000012641 Pigmentation disease Diseases 0.000 description 1
- 108010009736 Protein Hydrolysates Proteins 0.000 description 1
- 208000019155 Radiation injury Diseases 0.000 description 1
- 206010072170 Skin wound Diseases 0.000 description 1
- 101710172711 Structural protein Proteins 0.000 description 1
- 102000004142 Trypsin Human genes 0.000 description 1
- 108090000631 Trypsin Proteins 0.000 description 1
- 230000006750 UV protection Effects 0.000 description 1
- 229930003268 Vitamin C Natural products 0.000 description 1
- 229930003427 Vitamin E Natural products 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 238000004115 adherent culture Methods 0.000 description 1
- 235000001014 amino acid Nutrition 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 230000003698 anagen phase Effects 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 230000003064 anti-oxidating effect Effects 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 235000006708 antioxidants Nutrition 0.000 description 1
- 239000002216 antistatic agent Substances 0.000 description 1
- 230000017531 blood circulation Effects 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 1
- 230000003197 catalytic effect Effects 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 230000024245 cell differentiation Effects 0.000 description 1
- 230000032823 cell division Effects 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 229910052729 chemical element Inorganic materials 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 230000037319 collagen production Effects 0.000 description 1
- 229960002424 collagenase Drugs 0.000 description 1
- 210000002808 connective tissue Anatomy 0.000 description 1
- 230000002596 correlated effect Effects 0.000 description 1
- 230000000875 corresponding effect Effects 0.000 description 1
- 210000000805 cytoplasm Anatomy 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000008021 deposition Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 230000001079 digestive effect Effects 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 230000037336 dry skin Effects 0.000 description 1
- 230000009982 effect on human Effects 0.000 description 1
- 229920002549 elastin Polymers 0.000 description 1
- 210000002615 epidermis Anatomy 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 230000006355 external stress Effects 0.000 description 1
- 210000002744 extracellular matrix Anatomy 0.000 description 1
- 239000012894 fetal calf serum Substances 0.000 description 1
- 210000000245 forearm Anatomy 0.000 description 1
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 1
- WIGCFUFOHFEKBI-UHFFFAOYSA-N gamma-tocopherol Natural products CC(C)CCCC(C)CCCC(C)CCCC1CCC2C(C)C(O)C(C)C(C)C2O1 WIGCFUFOHFEKBI-UHFFFAOYSA-N 0.000 description 1
- 239000004519 grease Substances 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 229920002674 hyaluronan Polymers 0.000 description 1
- 229960003160 hyaluronic acid Drugs 0.000 description 1
- 230000003301 hydrolyzing effect Effects 0.000 description 1
- 210000002865 immune cell Anatomy 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 230000007794 irritation Effects 0.000 description 1
- 210000002510 keratinocyte Anatomy 0.000 description 1
- 230000031700 light absorption Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- WPBNNNQJVZRUHP-UHFFFAOYSA-L manganese(2+);methyl n-[[2-(methoxycarbonylcarbamothioylamino)phenyl]carbamothioyl]carbamate;n-[2-(sulfidocarbothioylamino)ethyl]carbamodithioate Chemical compound [Mn+2].[S-]C(=S)NCCNC([S-])=S.COC(=O)NC(=S)NC1=CC=CC=C1NC(=S)NC(=O)OC WPBNNNQJVZRUHP-UHFFFAOYSA-L 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000011733 molybdenum Substances 0.000 description 1
- 229910052750 molybdenum Inorganic materials 0.000 description 1
- 210000002569 neuron Anatomy 0.000 description 1
- 230000003472 neutralizing effect Effects 0.000 description 1
- 238000011580 nude mouse model Methods 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 230000036542 oxidative stress Effects 0.000 description 1
- 239000003002 pH adjusting agent Substances 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 239000003755 preservative agent Substances 0.000 description 1
- 230000002335 preservative effect Effects 0.000 description 1
- 230000000750 progressive effect Effects 0.000 description 1
- 230000002633 protecting effect Effects 0.000 description 1
- 230000004224 protection Effects 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 230000008439 repair process Effects 0.000 description 1
- 230000037384 skin absorption Effects 0.000 description 1
- 231100000274 skin absorption Toxicity 0.000 description 1
- 230000009759 skin aging Effects 0.000 description 1
- 230000005808 skin problem Effects 0.000 description 1
- 230000036548 skin texture Effects 0.000 description 1
- 238000011895 specific detection Methods 0.000 description 1
- 238000002798 spectrophotometry method Methods 0.000 description 1
- 230000004936 stimulating effect Effects 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 238000007920 subcutaneous administration Methods 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 230000008719 thickening Effects 0.000 description 1
- 239000002562 thickening agent Substances 0.000 description 1
- 239000003053 toxin Substances 0.000 description 1
- 231100000765 toxin Toxicity 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 239000012588 trypsin Substances 0.000 description 1
- 230000007306 turnover Effects 0.000 description 1
- 230000002792 vascular Effects 0.000 description 1
- 235000019154 vitamin C Nutrition 0.000 description 1
- 239000011718 vitamin C Substances 0.000 description 1
- 235000019165 vitamin E Nutrition 0.000 description 1
- 229940046009 vitamin E Drugs 0.000 description 1
- 239000011709 vitamin E Substances 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
- 230000029663 wound healing Effects 0.000 description 1
- 230000037314 wound repair Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/02—Cosmetics or similar toiletry preparations characterised by special physical form
- A61K8/04—Dispersions; Emulsions
- A61K8/042—Gels
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/02—Cosmetics or similar toiletry preparations characterised by special physical form
- A61K8/14—Liposomes; Vesicles
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/34—Alcohols
- A61K8/345—Alcohols containing more than one hydroxy group
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/35—Ketones, e.g. benzophenone
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/40—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing nitrogen
- A61K8/41—Amines
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/40—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing nitrogen
- A61K8/44—Aminocarboxylic acids or derivatives thereof, e.g. aminocarboxylic acids containing sulfur; Salts; Esters or N-acylated derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
- A61K8/645—Proteins of vegetable origin; Derivatives or degradation products thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
- A61K8/731—Cellulose; Quaternized cellulose derivatives
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
- A61K8/735—Mucopolysaccharides, e.g. hyaluronic acid; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/81—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds obtained by reactions involving only carbon-to-carbon unsaturated bonds
- A61K8/8141—Compositions of homopolymers or copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and at least one being terminated by only one carboxyl radical, or of salts, anhydrides, esters, amides, imides or nitriles thereof; Compositions of derivatives of such polymers
- A61K8/8147—Homopolymers or copolymers of acids; Metal or ammonium salts thereof, e.g. crotonic acid, (meth)acrylic acid; Compositions of derivatives of such polymers
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9771—Ginkgophyta, e.g. Ginkgoaceae [Ginkgo family]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/84—Products or compounds obtained by lyophilisation, freeze-drying
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- General Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Birds (AREA)
- Epidemiology (AREA)
- Botany (AREA)
- Dermatology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Engineering & Computer Science (AREA)
- Emergency Medicine (AREA)
- Mycology (AREA)
- Gerontology & Geriatric Medicine (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Chemical & Material Sciences (AREA)
- Dispersion Chemistry (AREA)
- Medicines Containing Plant Substances (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Cosmetics (AREA)
Abstract
本发明公开了一种护肤凝胶及其制备方法。属于日化技术领域。该凝胶原料包括透明质酸钠、甘油、燕麦β‑葡聚糖、卡波姆、羟乙基纤维素、1，2‑己二醇、对羟基苯乙酮、三乙醇胺、沙棘果提取物、苦参根提取物、红景天提取物、银杏提取物、野大豆芽提取物、谷氨酸、蛋氨酸、酵母菌多肽类和人生长激素HGH。该凝胶通过按照一定顺序将各原料在一定温度下混合均质制备而成。与现有技术相比，本发明取得的有益效果：无副作用；利于肌肤吸收；减轻细胞过氧化的程度并抑制I型胶原降解，修复细胞损伤；促进成纤维细胞和胶原蛋白的生成，有效修复细胞，增强皮肤弹性；保湿功能好；有效保持了人生长激素HGH的生物活性和生物利用度，增强了稳定性。
Description
技术领域
本发明涉及日化技术领域，更具体的说是涉及一种护肤凝胶及其制备方法。
背景技术
随着年龄的增长，人体角质层的更新率变得越来越慢，同时角质细胞的脂质合成能力下降，或表皮中的细胞***、生长和分化效率变低。由于皮肤缺少保湿因子和脂质，角质层屏障功能日趋下降。当角质层水分含量低于10％时，皮肤就会显得干燥、失去弹性、起皱，进而加速皮肤老化。另外，随着年龄增长，人体皮肤自身各种调节新陈代谢的激素的分泌减少，免疫细胞的功能及活性下降，这将导致构成活体的免疫蛋白和结构蛋白的合成减少。构成皮肤的胶原蛋白、弹性蛋白、透明质酸及糖蛋白的含量和排布改变或减少，且由于自由基和活性氧而发生氧化应激。皮肤变薄、皱纹增多、皮肤弹性下降、肤色变得暗沉，各种皮肤问题频繁出现。
目前，关于护肤凝胶的产品在市面上流通种类较多，市场上的凝胶主要分为两类，单一功效型和全效修复型。而市场上的凝胶为了更好地具有美容、防紫外线等功能，往往添加一些化学元素，带有一些毒副作用；另外，还有生物利用率低的缺陷，大部分活性物还没来得及到达作用部位，就被吸收甚至只停留在肌肤表面无法渗透。
综上，如何提供一种无毒副作用、生物利用度高、保湿修复效果好的护肤凝胶是本领域技术人员亟需解决的问题。
发明内容
有鉴于此，本发明提供了一种护肤凝胶及其制备方法。
为了实现上述目的，本发明采用如下技术方案：
一种护肤凝胶，包括如下质量份数的原料：透明质酸钠1～10份、甘油5～20份、燕麦β-葡聚糖5～10份、卡波姆0.5～1份、羟乙基纤维素0.1～0.5份、1，2-己二醇0.1～0.3份、对羟基苯乙酮0.1～0.8份、三乙醇胺0.1～0.5份、沙棘果提取物3～8份、苦参根提取物5～10份、红景天提取物2～6份、银杏提取物1～3份、野大豆芽提取物1～5份、谷氨酸0.5～2份、蛋氨酸0.5～2份、酵母菌多肽类8～23份和人生长激素HGH 25～35份
透明质酸钠是构成细胞间和细胞外基质的主要成分，维持细胞的正常结构和功能。透明质酸钠能结合其重量1000倍的水，其保水性能与其浓度及分子量呈正相关。能起到独特的保护皮肤作用，可保持皮肤滋润光滑、细腻柔嫩、富有弹性，具有防皱、抗皱、修复皮肤生理功能的作用。
甘油可以吸收空气中的水分来滋润皮肤，并在肌肤表面形成一层锁水膜，减缓皮肤内水分的流失，让皮肤长时间的保持水润的状态，改善干燥的皮肤。
燕麦β-葡聚糖具有抗衰老功效，能够抚平细小皱纹，提高皮肤弹性，改善皮肤纹理度，其独特的直链分子结构赋予其良好的透皮吸收性能，促进成纤维细胞合成胶原蛋白，促进伤口愈合，修复受损肌肤。
卡波姆是一种增稠剂，能增强护肤品的粘度。并且可以保护肌肤，减少刺激性，抗紫外线，杀菌消炎，缓解肌肤伤口。
羟乙基纤维素的溶解性和粘度性能充分的发挥作用，保持平衡，使在冷热交替的季节也能保持化妆品的稳定性，并且其具有保湿性能。
1，2-己二醇具有极高的吸湿性、保水性和抗菌性。
对羟基苯乙酮是一种高温辅助活性稳定剂，作为防腐剂存在于化妆品之中，对人体皮肤无害。
三乙醇胺是含有卡波姆等酸性高分子凝胶的中和剂，通过与卡波姆等羧基中和，形成稳定的高分子结构，达到增稠和保湿的效果。
沙棘果提取物含有大量的维生素C、维生素E以及黄酮，能够有效防止自由基的衰老。
苦参根提取物能够平衡油脂分泌，疏通并收敛毛孔，清除皮肤内毒素杂质，促进受损血管神经细胞的生长和修复，恢复皮下毛细血管细胞活力，使肌肤重现紧致细滑。
红景天提取物(1)对真皮中成纤维细胞产生了刺激作用，促进了成纤维细胞的***及其合成和分泌胶原蛋白同时也分泌胶原酶，使原有的胶原分解，但总的分泌量大于分解量，胶原蛋白在细胞外组成胶原纤维，而胶原纤维含量的增加说明红景天对皮肤有一定的抗老化作用；(2)通过抑制酪氨酸酶的活性，降低其催化速度，从而能够减少皮肤黑色素的生成，改善皮肤色素沉着状态，达到关白的功效；(3)对皮肤紫外线辐射损伤具有保护作用。
银杏提取物中所含有的黄酮成分可以阻碍色素在真皮层的形成与沉着，达到关白肌肤与防治色素斑块的作用，除了黄酮外，其含有的锰、钼等微量元素，也能清除氧自由基及抑制黑色素生长；银杏提取物也能保护真皮层细胞，改善血液循环，防止细胞被氧化产生皱纹。
野大豆芽提取物能够促进纤维芽细胞的生长，具有活肤、抗衰老的作用；同时能保持皮肤的水分，有保湿功效。
谷氨酸一般用作化妆品的pH调节剂和营养助剂。
蛋氨酸在化妆品中作抗静电剂、抗氧化剂和皮肤调理剂，该成分为水溶性成分，能促进皮肤新陈代谢，刺激细胞新生。
酵母菌多肽类可以保护肌肤不受外界应激的损伤，使肌肤衍生出自然健康的白。
人生长激素HGH可以是皮肤富有弹性。
进一步的，一种护肤凝胶，包括如下质量份数的原料：透明质酸钠5份、甘油13份、燕麦β-葡聚糖7份、卡波姆0.8份、羟乙基纤维素0.3份、1，2-己二醇0.2份、对羟基苯乙酮0.5份、三乙醇胺0.3份、沙棘果提取物5份、苦参根提取物7份、红景天提取物4份、银杏提取物2份、野大豆芽提取物3份、谷氨酸1份、蛋氨酸1份、酵母菌多肽类13份和人生长激素HGH 30份。
一种护肤凝胶的制备方法，人生长激素HGH经大分子包裹步骤，得到预处理人生长激素HGH，具体操作为：
(1)将人生长激素HGH经纳米脂质体包裹包封技术制成脂质体；
(2)将所述脂质体用海藻糖作为赋形剂制成冻干粉，即得到预处理人生长激素HGH。
采用纳米脂质体包裹透皮吸收技术，并且以海藻糖为赋形剂制备为冻干粉，解决了大分子蛋白难以透过皮肤天然屏障充分吸收的问题。具有修复护理皮肤，延缓衰老和抗衰老的功能。采用海藻糖为赋形剂，避免了生物多肽蛋白活性物质在普通条件下长期使用多肽蛋白物质失去活性，有效保持了人生长激素HGH强大的生物活性和生物利用度，增强了稳定性。
优选的，包括如下步骤：
(1)将水、透明质酸钠、甘油、燕麦β-葡聚糖加入反应釜中，开启搅拌，加热至75～80℃，得混合物A；
(2)将水、卡波姆、羟乙基纤维素、1，2-己二醇、对羟基苯乙酮、三乙醇胺加入反应釜中，开启搅拌，加热至75～80℃，得混合物B；
(3)将混合物A和混合物B混合，10000～12000rpm/min均质5min，降温至40～45℃，加入沙棘果提取物、苦参根提取物、红景天提取物、银杏提取物、野大豆芽提取物、谷氨酸、蛋氨酸、酵母菌多肽类和预处理人生长激素HGH，3000～5000rpm/min均质1min；
(4)继续降温至25～30℃即可。
经由上述的技术方案可知，与现有技术相比，本发明取得的有益效果为：(1)安全无副作用；(2)质地清透，利于肌肤吸收；(3)减轻细胞过氧化的程度并抑制I型胶原的降解，有修复皮肤细胞损伤的功效；(4)促进成纤维细胞和胶原蛋白的生成，有效修复细胞，增强皮肤弹性，改善干纹、粗糙；(5)保湿功能好，使皮肤角质层含水量在1小时后具有很大的提高，并且在使用后4小时能得到较好的维持；(6)有效保持了人生长激素HGH强大的生物活性和生物利用度，增强了稳定性。
具体实施方式
下面将对本发明实施例中的技术方案进行清楚、完整地描述，显然，所描述的实施例仅仅是本发明一部分实施例，而不是全部的实施例。基于本发明中的实施例，本领域普通技术人员在没有做出创造性劳动前提下所获得的所有其他实施例，都属于本发明保护的范围。
沙棘果提取物购自宁陕国圣生物科技有限公司；
苦参根提取物购自河南康之旺生物科技有限公司；
红景天提取物购自陕西帕尼尔生物科技有限公司；
银杏提取物购自西安绿天生物技术有限公司；
野大豆芽提取物购自宁陕国圣生物科技有限公司；
酵母菌多肽类购自广州诗茗化工有限公司。
本发明未提及材料采购自市售渠道；未提及的实验方法为常规实验方法，在此不再一一赘述。
实施例1
产品配方：透明质酸钠1kg、甘油5kg、燕麦β-葡聚糖5kg、卡波姆0.5kg、羟乙基纤维素0.1kg、1，2-己二醇0.1kg、对羟基苯乙酮0.1kg、三乙醇胺0.1kg、沙棘果提取物3kg、苦参根提取物5kg、红景天提取物2kg、银杏提取物1kg、野大豆芽提取物1kg、谷氨酸0.5kg、蛋氨酸0.5kg、酵母菌多肽类8kg和人生长激素HGH 25kg。
制备方法：
(1)将水、透明质酸钠、甘油、燕麦β-葡聚糖加入反应釜中，开启搅拌，加热至75℃，得混合物A；
(2)将水、卡波姆、羟乙基纤维素、1，2-己二醇、对羟基苯乙酮、三乙醇胺加入反应釜中，开启搅拌，加热至75℃，得混合物B；
(3)将混合物A和混合物B混合，10000rpm/min均质5min，降温至40℃，加入沙棘果提取物、苦参根提取物、红景天提取物、银杏提取物、野大豆芽提取物、谷氨酸、蛋氨酸、酵母菌多肽类和预处理人生长激素HGH(将1份人生长激素HGH用纳米脂质体制备法进行加工制成脂质体，然后添加500份海藻糖、适量稳定剂通过冻干技术制备而成，即得预处理人生长激素HGH)，3000rpm/min均质1min；
(4)继续降温至25℃即可。
实施例2
产品配方：透明质酸钠10kg、甘油20kg、燕麦β-葡聚糖10kg、卡波姆1kg、羟乙基纤维素0.5kg、1，2-己二醇0.3kg、对羟基苯乙酮0.8kg、三乙醇胺0.5kg、沙棘果提取物8kg、苦参根提取物10kg、红景天提取物6kg、银杏提取物3kg、野大豆芽提取物5kg、谷氨酸2kg、蛋氨酸2kg、酵母菌多肽类23kg和人生长激素HGH 35kg。
制备方法：
(1)将水、透明质酸钠、甘油、燕麦β-葡聚糖加入反应釜中，开启搅拌，加热至80℃，得混合物A；
(2)将水、卡波姆、羟乙基纤维素、1，2-己二醇、对羟基苯乙酮、三乙醇胺加入反应釜中，开启搅拌，加热至80℃，得混合物B；
(3)将混合物A和混合物B混合，12000rpm/min均质5min，降温至45℃，加入沙棘果提取物、苦参根提取物、红景天提取物、银杏提取物、野大豆芽提取物、谷氨酸、蛋氨酸、酵母菌多肽类和预处理人生长激素HGH(将1份人生长激素HGH用纳米脂质体制备法进行加工制成脂质体，然后添加500份海藻糖、适量稳定剂通过冻干技术制备而成，即得预处理人生长激素HGH)，5000rpm/min均质1min；
(4)继续降温至30℃即可。
实施例3
产品配方：透明质酸钠5kg、甘油13kg、燕麦β-葡聚糖7kg、卡波姆0.8kg、羟乙基纤维素0.3kg、1，2-己二醇0.2kg、对羟基苯乙酮0.5kg、三乙醇胺0.3kg、沙棘果提取物5kg、苦参根提取物7kg、红景天提取物4kg、银杏提取物2kg、野大豆芽提取物3kg、谷氨酸1kg、蛋氨酸1kg、酵母菌多肽类13kg和人生长激素HGH 30kg。
制备方法：
(1)将水、透明质酸钠、甘油、燕麦β-葡聚糖加入反应釜中，开启搅拌，加热至80℃，得混合物A；
(2)将水、卡波姆、羟乙基纤维素、1，2-己二醇、对羟基苯乙酮、三乙醇胺加入反应釜中，开启搅拌，加热至75℃，得混合物B；
(3)将混合物A和混合物B混合，10000rpm/min均质5min，降温至45℃，加入沙棘果提取物、苦参根提取物、红景天提取物、银杏提取物、野大豆芽提取物、谷氨酸、蛋氨酸、酵母菌多肽类和预处理人生长激素HGH(将1份人生长激素HGH用纳米脂质体制备法进行加工制成脂质体，然后添加500份海藻糖、适量稳定剂通过冻干技术制备而成，即得预处理人生长激素HGH)，5000rpm/min均质1min；
(4)继续降温至25℃即可。
对比例1
不添加人生长激素HGH，其余操作同实施例3。
实验1对皮肤HaCaT细胞的抗氧化作用实验
(1)检测对象：实施例1、实施例2、实施例3、对比例1制备的凝胶；
(2)检测指标：SOD、T-AOC、Hyp、CAT；
(3)检测方法：
①细胞处理：取对数生长期的HaCaT细胞，经0.25％胰蛋白酶消化，用DMEM完全培养基制成密度为1×105个/ml细胞的细胞悬液，加入96孔培养板，每孔100μl，贴壁培养；
②作用24h后取细胞培养上清液，12000r/min离心5min，取上清，按试剂盒说明书方法检测，实验重复3次并设置空白对照。
(4)实验结果：实验结果如表1所示。
表1抗氧化指标结果
| SOD | T-AOC | Hyp | CAT | |
| 空白对照 | 29.42 | 8.21 | 3.18 | 0.65 |
| 实施例1 | 147.35 | 32.81 | 11.47 | 6.56 |
| 实施例2 | 145.39 | 33.45 | 10.58 | 6.79 |
| 实施例3 | 146.96 | 33.07 | 11.96 | 6.02 |
| 对比例1 | 98.57 | 17.24 | 7.35 | 4.06 |
实验结果表明，本发明凝胶作用组细胞CAT、SOD、Hyp和T-AOC均比空白对照组高。说明本发明制备的凝胶可以减轻细胞过氧化的程度并抑制I型胶原的降解，有修复皮肤细胞损伤的功效。
实验2人皮肤成纤维细胞增殖活性实验
(1)检测对象：实施例1、实施例2、实施例3、对比例1制备的凝胶；
(2)检测方法：
人皮肤成纤维细胞加入DMEM培养基(含5％胎牛血清)，置于含5％CO2，湿度为95％，恒温37℃的二氧化碳培养箱中培养，培养48h后更换培养液，待细胞长满培养品，取出弃去培养液，加入消化液约3mL，以覆满瓶底为限，约2-3分钟后，当发现细胞质回缩，细胞间隙增大后，加入DMEM培养基终止反应，并制备成单细胞悬液。5000个/mL浓度接种于96孔板，每孔加入100μL。置于含5％CO2，湿度为95％，恒温37℃的二氧化碳培养箱中培养，待细胞附着后，更换100μL培养液，同时分别添加样品100μL，对照组加入相同体积PBS缓中液，每组设置4个平行孔继续置于培养箱培养，检测48h的OD值。
具体检测步骤：
每孔加入MTT溶液10μL，震荡显色，继续培养4h，将96孔板的培养上清弃除，再加入DMSO 100μL，摇床上震荡10min，酶标仪570nm测定OD值。
(3)检测结果：检测结果如表2所示。
表2对人皮肤成纤维细胞增殖活性的影响
| 对照组 | 实施例1 | 实施例2 | 实施例3 | 对比例1 | |
| OD(48h) | 0.403 | 0.647 | 0.639 | 0.629 | 0.521 |
由表2结果可看出，本发明制备的凝胶能够促进成纤维细胞的生成，成纤维细胞是疏松***的主要细胞成分，属于终末期分化细胞，这种细胞可制造胶原蛋白等蛋白质。根据数据，可以得知，从0h到48h，成纤维细胞的生长呈递增状态，本发明凝胶可以通过促进细胞的生长来达到修复肌肤的功效。
实验3促进胶原蛋白形成的实验
(1)检测对象：实施例1、实施例2、实施例3、对比例1制备的凝胶；
(2)检测原理：羟脯氨酸是胶原蛋白的一种特有氨基酸，常以羟脯氨酸的含量换算胶原蛋白含量。采用分光光度法测定羟脯氨酸(HYP)含量，按胶原蛋白中14％的HYP换算小鼠皮肤中的胶原蛋白含量；
(3)检测方法：
在裸鼠右侧耳背涂抹样品，每日早晚各一次涂抹，用药1g。左侧不用药作为对照组。在清洁、通风、干燥、室温22±2℃条件下，相同饲料喂养。用药30天后各取5只，处死，取用其药物作用部位及对侧相应部位皮肤。
用天平称组织湿重45mg放入洁净试管中，加入羟脯氨酸测定试剂盒中水解液1mL，混匀。加盖沸水浴水解20min，每隔10min混匀一次，目的让水解更加充分。取出试管，冷却后往各管加入试剂盒的指示剂1滴，摇匀。再加入试剂盒中甲液1mL调pH，摇匀。然后采用加样器吸取调pH的乙液，逐滴加入各试管中，直至看到各管中的指示剂变成黄绿色。加入蒸馏水至10mL后混匀。取3mL的水解液加入20-30mg活性炭后混匀，3500转/min，离心10min，取上清1mL。
取干净试管加入5μg/mL标准应用液1mL作为标准管。往各管中加入试剂1后混匀，静置10min，再加入试剂2，混匀静置5min，加入试剂3，混匀60℃水浴15min，冷却后3500转/min，离心10min，取上清于550nm，1cm光径，测吸光值。
(4)检测结果：检测结果如表3所示。
表3对小鼠皮肤中胶原蛋白生成的影响
| 对照组 | 实施例1 | 实施例2 | 实施例3 | 对比例1 | |
| 胶原蛋白含量(30天) | 11.6 | 25.3 | 25.9 | 24.8 | 16.5 |
实验4保湿功效评价
(1)测试样品：实施例1制备的凝胶；
(2)测试指标：皮肤角质层含水量变化率；
(3)测试方法：
该实验包括40位志愿者，年龄在21-45周岁。在试验期间按规定使用实施例1制备的凝胶。实验前，统一清洗双手前臂内侧，做好测量标记。先测量各区域的初始值(T0)，再将测试样品按规定量单次涂布。在1h和4h后使用皮肤水分测试仪进行产品区域和对照区域的测量，记录为Th。计算公式如下：
皮肤角质层含水量变化率＝[(Th-T0)-(Th空白-T0空白)]/T0；
(4)测试结果：使用1h后皮肤角质层含水量变化率为70.2％，使用4h后皮肤角质层含水量变化率为40.6，说明本发明实施例1制备的凝胶使皮肤角质层含水量在1小时后具有很大的提高，并且在使用后4小时能得到较好的维持。
本说明书中各个实施例采用递进的方式描述，每个实施例重点说明的都是与其他实施例的不同之处，各个实施例之间相同相似部分互相参见即可。
对所公开的实施例的上述说明，使本领域专业技术人员能够实现或使用本发明。对这些实施例的多种修改对本领域的专业技术人员来说将是显而易见的，本文中所定义的一般原理可以在不脱离本发明的精神或范围的情况下，在其它实施例中实现。因此，本发明将不会被限制于本文所示的这些实施例，而是要符合与本文所公开的原理和新颖特点相一致的最宽的范围。
Claims (4)
1.一种护肤凝胶，其特征在于，包括如下质量份数的原料：透明质酸钠1～10份、甘油5～20份、燕麦β-葡聚糖5～10份、卡波姆0.5～1份、羟乙基纤维素0.1～0.5份、1，2-己二醇0.1～0.3份、对羟基苯乙酮0.1～0.8份、三乙醇胺0.1～0.5份、沙棘果提取物3～8份、苦参根提取物5～10份、红景天提取物2～6份、银杏提取物1～3份、野大豆芽提取物1～5份、谷氨酸0.5～2份、蛋氨酸0.5～2份、酵母菌多肽类8～23份和人生长激素HGH 25～35份。
2.如权利要求1所述的一种护肤凝胶，其特征在于，包括如下质量份数的原料：透明质酸钠5份、甘油13份、燕麦β-葡聚糖7份、卡波姆0.8份、羟乙基纤维素0.3份、1，2-己二醇0.2份、对羟基苯乙酮0.5份、三乙醇胺0.3份、沙棘果提取物5份、苦参根提取物7份、红景天提取物4份、银杏提取物2份、野大豆芽提取物3份、谷氨酸1份、蛋氨酸1份、酵母菌多肽类13份和人生长激素HGH 30份。
3.如权利要求1或2所述的一种护肤凝胶的制备方法，其特征在于，人生长激素HGH经大分子包裹步骤，得到预处理人生长激素HGH，具体操作为：
(1)将人生长激素HGH经纳米脂质体包裹包封技术制成脂质体；
(2)将所述脂质体用海藻糖作为赋形剂制成冻干粉，即得到预处理人生长激素HGH。
4.如权利要求3所述的一种护肤凝胶的制备方法，其特征在于，包括如下步骤：
(1)将水、透明质酸钠、甘油、燕麦β-葡聚糖加入反应釜中，开启搅拌，加热至75～80℃，得混合物A；
(2)将水、卡波姆、羟乙基纤维素、1，2-己二醇、对羟基苯乙酮、三乙醇胺加入反应釜中，开启搅拌，加热至75～80℃，得混合物B；
(3)将混合物A和混合物B混合，10000～12000rpm/min均质5min，降温至40～45℃，加入沙棘果提取物、苦参根提取物、红景天提取物、银杏提取物、野大豆芽提取物、谷氨酸、蛋氨酸、酵母菌多肽类和预处理人生长激素HGH，3000～5000rpm/min均质1min；
(4)继续降温至25～30℃即可。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010599999.1A CN111568781A (zh) | 2020-06-23 | 2020-06-23 | 一种护肤凝胶及其制备方法 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010599999.1A CN111568781A (zh) | 2020-06-23 | 2020-06-23 | 一种护肤凝胶及其制备方法 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN111568781A true CN111568781A (zh) | 2020-08-25 |
Family
ID=72116462
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010599999.1A Pending CN111568781A (zh) | 2020-06-23 | 2020-06-23 | 一种护肤凝胶及其制备方法 |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN111568781A (zh) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113797147A (zh) * | 2021-10-27 | 2021-12-17 | 上海圣岳生物科技有限公司 | 一种促进人脑垂体分泌hgh的凝胶及其制备方法 |
| CN115381761A (zh) * | 2022-08-12 | 2022-11-25 | 深圳阿尔法生物科技有限公司 | 一种海洋胶原蛋白修复霜 |
Citations (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1456147A (zh) * | 2003-06-04 | 2003-11-19 | 上海吉英生物科技有限公司 | 一种含有重组人生长素护理皮肤的化妆品 |
| US20070081963A1 (en) * | 2005-10-12 | 2007-04-12 | Regeron, Inc. | Composition for improving skin conditions comprising human growth hormone as an active ingredient |
| CN101190167A (zh) * | 2006-11-23 | 2008-06-04 | 陕西晶美生物科技有限公司 | 复合型生长因子护肤品原料及使用该原料的修复护肤品 |
| CN101249043A (zh) * | 2008-04-03 | 2008-08-27 | 冯来坤 | 人生长激素和人表皮生长因子组合物在美容中的应用 |
| CN101395179A (zh) * | 2006-03-06 | 2009-03-25 | 凯尔杰有限公司 | 具有***-i活性的肽及其用途 |
| CN101711729A (zh) * | 2009-12-24 | 2010-05-26 | 江西宇骏生物工程有限公司 | 含有脂质体重组人生长激素护理皮肤的化妆品及其制备方法 |
| CN102525867A (zh) * | 2012-01-16 | 2012-07-04 | 上海得高实业有限公司 | 鸡胚精华液冻干粉及其制备方法和应用 |
| KR20120139371A (ko) * | 2011-06-17 | 2012-12-27 | (주)케어젠 | 인간 성장호르몬 유래 펩타이드 및 이의 용도 |
| JP2014208711A (ja) * | 2014-08-11 | 2014-11-06 | リゼロン、インク． | ヒト成長ホルモンを有効成分として含む発毛の促進用の組成物 |
| CN105012933A (zh) * | 2015-08-18 | 2015-11-04 | 吉林大学 | 含有林蛙皮多肽的微创修复凝胶 |
| CN107496315A (zh) * | 2016-06-13 | 2017-12-22 | 欧诗漫生物股份有限公司 | 一种护肤凝胶及其制备方法 |
| CN108478475A (zh) * | 2018-05-05 | 2018-09-04 | 东莞美肤莱化妆品有限公司 | 一种青春定格原液 |
| CN110496067A (zh) * | 2019-09-29 | 2019-11-26 | 海口惠元生物科技有限公司 | 一种祛皱组合物及其制备方法和应用 |
-
2020
- 2020-06-23 CN CN202010599999.1A patent/CN111568781A/zh active Pending
Patent Citations (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1456147A (zh) * | 2003-06-04 | 2003-11-19 | 上海吉英生物科技有限公司 | 一种含有重组人生长素护理皮肤的化妆品 |
| US20070081963A1 (en) * | 2005-10-12 | 2007-04-12 | Regeron, Inc. | Composition for improving skin conditions comprising human growth hormone as an active ingredient |
| CN101395179A (zh) * | 2006-03-06 | 2009-03-25 | 凯尔杰有限公司 | 具有***-i活性的肽及其用途 |
| CN101190167A (zh) * | 2006-11-23 | 2008-06-04 | 陕西晶美生物科技有限公司 | 复合型生长因子护肤品原料及使用该原料的修复护肤品 |
| CN101249043A (zh) * | 2008-04-03 | 2008-08-27 | 冯来坤 | 人生长激素和人表皮生长因子组合物在美容中的应用 |
| CN101711729A (zh) * | 2009-12-24 | 2010-05-26 | 江西宇骏生物工程有限公司 | 含有脂质体重组人生长激素护理皮肤的化妆品及其制备方法 |
| KR20120139371A (ko) * | 2011-06-17 | 2012-12-27 | (주)케어젠 | 인간 성장호르몬 유래 펩타이드 및 이의 용도 |
| CN102525867A (zh) * | 2012-01-16 | 2012-07-04 | 上海得高实业有限公司 | 鸡胚精华液冻干粉及其制备方法和应用 |
| JP2014208711A (ja) * | 2014-08-11 | 2014-11-06 | リゼロン、インク． | ヒト成長ホルモンを有効成分として含む発毛の促進用の組成物 |
| CN105012933A (zh) * | 2015-08-18 | 2015-11-04 | 吉林大学 | 含有林蛙皮多肽的微创修复凝胶 |
| CN107496315A (zh) * | 2016-06-13 | 2017-12-22 | 欧诗漫生物股份有限公司 | 一种护肤凝胶及其制备方法 |
| CN108478475A (zh) * | 2018-05-05 | 2018-09-04 | 东莞美肤莱化妆品有限公司 | 一种青春定格原液 |
| CN110496067A (zh) * | 2019-09-29 | 2019-11-26 | 海口惠元生物科技有限公司 | 一种祛皱组合物及其制备方法和应用 |
Non-Patent Citations (4)
| Title |
|---|
| 刘宗利等: "海藻糖在日化领域的应用研究进展" * |
| 小美吖: "疤痕修复篇--HGH化妆品修复精华素在疤痕及肌肤修复的功效", 《豆瓣》 * |
| 王建新: "《化妆品植物原料大全》", 30 June 2012, 中国纺织出版社 * |
| 王昌涛等: "《化妆品植物添加剂的开发与应用》", 30 April 2013, 中国轻工业出版社 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113797147A (zh) * | 2021-10-27 | 2021-12-17 | 上海圣岳生物科技有限公司 | 一种促进人脑垂体分泌hgh的凝胶及其制备方法 |
| CN115381761A (zh) * | 2022-08-12 | 2022-11-25 | 深圳阿尔法生物科技有限公司 | 一种海洋胶原蛋白修复霜 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104173251B (zh) | 一种牦牛骨胶原肽美白面膜及其制备方法 | |
| CN111388346A (zh) | 一种植物蛋白与多肽配方的冻干粉活面膜及其制备方法 | |
| CN113576975B (zh) | 一种易吸收的抗衰老组合物及其制备方法和应用 | |
| KR101697152B1 (ko) | 장어로부터 추출한 뮤신을 이용한 항노화 기능을 가지는 화장품 제재의 제조방법 | |
| KR101098400B1 (ko) | 홍삼 먹인 달팽이 점액의 제조방법 및 이를 함유하는 화장료 조성물 | |
| CN111671704B (zh) | 一种化妆品组合物、化妆品及其制备方法 | |
| CN111568781A (zh) | 一种护肤凝胶及其制备方法 | |
| CN104887542B (zh) | 一种化妆品组合物 | |
| CN113171318A (zh) | 一种具有保湿美白舒缓修复的冻干粉制剂及制备方法 | |
| CN108852969B (zh) | 一种抗皱抗衰老美白面膜及其制备方法 | |
| CN110585111A (zh) | 一种抗衰老化妆品添加物及其制备方法 | |
| CN111632003A (zh) | 一种具有美白功效的化妆品组合物 | |
| CN117297996A (zh) | 一种水蛭素冻干面膜及其制备方法 | |
| CN112972359B (zh) | 一种微脂囊包裹的化妆品用组合物及其在芙蓉霜中的应用 | |
| CN113116757B (zh) | 胶原酶抑制剂，含该胶原酶抑制剂的补水面膜和制备方法 | |
| KR101219293B1 (ko) | 최적 농도의 카테킨과 데아닌을 함유하는 피부 외용제조성물 | |
| CN114159341A (zh) | 一种冻干眼贴及其制备方法 | |
| KR20120119520A (ko) | 대나무수액의 균발효 추출물을 함유하는 피부외용제 조성물 | |
| CN108113916B (zh) | 一种天然修复肽面膜液及其制备方法 | |
| CN111514064A (zh) | 一种西林瓶冻干面膜及其制备方法 | |
| CN110200863A (zh) | 一种医用修复型面膜及其制备方法 | |
| CN115444772B (zh) | 一种保湿美白组合物及其在射频美容和医美护肤中的应用 | |
| CN117017833B (zh) | 具有多效性修护抗衰功效的复合物及制备方法 | |
| CN108815018A (zh) | 一种海狸鼠尾筋提取胶原蛋白肽的面膜液及其制备方法 | |
| CN115624510B (zh) | 一种美白精华液及其制备方法 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20200825 |
|
| RJ01 | Rejection of invention patent application after publication |