CN111567519A - Preparation method of biological disinfectant containing silver ion water - Google Patents

Preparation method of biological disinfectant containing silver ion water Download PDF

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CN111567519A
CN111567519A CN202010275795.2A CN202010275795A CN111567519A CN 111567519 A CN111567519 A CN 111567519A CN 202010275795 A CN202010275795 A CN 202010275795A CN 111567519 A CN111567519 A CN 111567519A
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silver ion
biological
ion water
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陈龙
吴鹏
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Ningxia Yizheng Biological Engineering Co ltd
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Ningxia Yizheng Biological Engineering Co ltd
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    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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Abstract

The invention discloses a preparation method of a biological disinfectant containing silver ion water, which comprises the following components in percentage by mass: 10-40 parts of biological enzyme composition, 30-50 parts of traditional Chinese medicine extract chelating liquid, 1-5 parts of nisin, 1-5 parts of calcium propionate and 10-40 parts of silver ion water. The disinfectant is mainly characterized in that a novel sterilization method is developed aiming at the problems that a chemical disinfectant has large residue and is easy to cause accumulation poisoning, and simultaneously silver ion electrolyzed water, traditional Chinese medicine extract, nisin, calcium propionate and other biological enzymes with sterilization effects are added to act on bacteria, fungi, viruses and other microorganisms together, so that the disinfectant has the characteristics of high safety, quick sterilization, wide sterilization range, no generation of pungent odor and other high-efficiency sterilization.

Description

Preparation method of biological disinfectant containing silver ion water
Technical Field
The invention relates to a preparation method of a biological disinfectant containing silver ion water, belonging to the field of biological disinfection.
Technical Field
The disinfectant aims at killing and controlling harmful microbes in the external environment, is an important measure for effectively cutting off the transmission path of infectious diseases, but the disinfectant often generates toxic and side effects, has strong irritation to chemical disinfectants, and is frequently inhaled to cause chronic poisoning and the like.
The domestic disinfection on the market is roughly divided into three types of physical disinfection, chemical disinfection and biological disinfection according to different methods, but from the practical use, the disinfection has different defects respectively. The common physical disinfection mainly comprises heat, ultraviolet rays, ultrasonic waves, microwaves, ionizing radiation and the like, and most of the common physical disinfection requires a special generating device. Chemical disinfection is to kill pathogenic microorganisms by using chemical drugs, and although the chemical disinfectant has a wide bactericidal spectrum, the chemical disinfectant generally has the harm of disinfectant residues after disinfection. Therefore, in recent years, chemical disinfectants have been intensively studied on how to modify them by improved means to achieve environmental friendliness. The biological disinfection method is to directly use some substances generated by organisms or indirectly use extracts of the organisms to kill pathogenic microorganisms. The biological disinfectant mainly comprises a disinfectant which takes biological enzyme, bacteriophage, plant extract, antibacterial peptide and the like as main components, can inhibit or kill pests, but cannot generate continuous destructive effect on the environment, and has the defects that the traditional biological disinfection technology generally directly uses organisms for disinfection, the action process is slow, and some problems exist in practical application, such as: the killing effect on the harmful organisms is difficult to determine, the disinfection efficiency is not high, and the large-scale application is not facilitated.
Therefore, in order to search for a safe, low-toxicity, high-efficiency, fast and broad-spectrum disinfectant, research on the disinfectant in recent years at home and abroad is mainly focused on the aspects. Such as developing new biological methods, researching the compounding technology of disinfectants, utilizing the synergistic effect of physicochemical factors, developing preparation technology and application technology, researching new disinfection mechanisms and the like.
Disclosure of Invention
Based on this, there is a need for a method for preparing a biological disinfectant containing silver ion water.
The preparation method of the biological disinfectant containing silver ion water is characterized in that the formula comprises the following components in percentage by mass: 10-40 parts of biological enzyme composition, 30-50 parts of traditional Chinese medicine extract chelating liquid, 1-5 parts of nisin, 1-5 parts of calcium propionate and 10-40 parts of silver ion water.
The preparation method comprises the following steps:
(1) preparing a biological enzyme composition: mixing lysozyme, carboxypeptidase, collagen hydrolase, catalase and glucose oxidase according to mass fraction, adding silver ion water, filtering, and taking filtrate for later use;
(2) traditional Chinese medicine extraction: mixing folium Artemisiae Argyi, flos Lonicerae, herba Taraxaci, fructus forsythiae, and Glycyrrhrizae radix according to mass fraction, adding 95% ethanol, extracting for 3 times, each for 1 hr, mixing extractive solutions, cooling, filtering, adding decolorizing agent for decolorizing, recovering solvent, concentrating to density of 1.3g/ml, and naming as extractive solution I;
(3) adding 3 times of water into the dregs of a decoction extracted in the step (2), extracting for 3 times at 90 ℃ for 1 hour each time, combining the extracting solutions, cooling, filtering, adding a decoloring agent for decoloring, and naming the extracting solution as an extracting solution II;
(4) mixing the extracting solution I and the extracting solution II according to the mass fraction to obtain a traditional Chinese medicine extract;
(5) adding chitosan with deacetylation degree of 90% in an amount of 2% by mass into the Chinese medicinal extract, chelating with the Chinese medicinal extract for 1 hr at 60 deg.C, centrifuging, and collecting supernatant to obtain Chinese medicinal extract chelating solution; (ii) a
(6) Mixing the biological enzyme composition, the Chinese medicinal extract chelating solution, nisin, calcium propionate and silver ion water according to mass fraction, adjusting the pH value to 6.5-7.5, stirring and filling to obtain the biological disinfectant containing silver ion water.
Preferably, the biological enzyme composition comprises the following components in percentage by mass: 3-5 parts of lysozyme, 1-3 parts of carboxypeptidase, 1-3 parts of collagen hydrolase, 1-3 parts of catalase and 1-3 parts of glucose oxidase.
Preferably, the traditional Chinese medicine composition comprises the following components in percentage by mass: 3-5 parts of folium artemisiae argyi, 1-4 parts of honeysuckle, 1-3 parts of dandelion, 1-3 parts of fructus forsythiae and 1-3 parts of liquorice.
Preferably, the silver ion water is water prepared by electrolyzing silver ions, has effects of sterilizing and disinfecting, and has silver ion content of 50-80 ppm.
Preferably, the adding amount of the silver ion water is 3 to 5 times of the mass fraction of the biological enzyme composition.
Preferably, the decolorizing agent is activated carbon, and the addition amount of the decolorizing agent is 2-5 per mill of the mass fraction of the extracting solution.
Preferably, the extracting solution I and the extracting solution II are mixed according to the mass fraction of 7: 3.
Preferably, the mixing and stirring time is 30 min.
Preferably, the biological disinfectant containing silver ion water is used by diluting the stock solution by 100 times.
The lysozyme, the carboxypeptidase, the collagen hydrolase, the catalase and the glucose oxidase are all commercially available commodities, and the folium artemisiae argyi, the honeysuckle, the dandelion, the fructus forsythiae and the liquorice are also commercially available commodities.
Compared with other methods, the method has the following beneficial effects:
1. silver ion is an inorganic antibacterial substance, silver element is electrolyzed by adding water through equipment to form Ag + by the electrolysis, the antibacterial principle is that the silver element is firmly adsorbed on a cell membrane by coulomb attraction and breaks down cell walls to enter cells to solidify cell proteins, and the cells die due to the loss of division and proliferation capacity. In addition, the silver ion Ag + can also damage a microorganism electron transmission system, a respiratory system and a substance transmission system, and when the thallus loses activity, the silver ion Ag + is dissociated from the thallus and is repeatedly sterilized, so that the antibacterial effect is durable.
2. The disinfectant is prepared from egg white as main component, and lysozyme, also called muramidase or N-acetylmuramidase hydrolase, is an alkaline enzyme capable of hydrolyzing mucopolysaccharide in bacteria. The principle is that the insoluble mucopolysaccharide of the cell wall is decomposed into soluble glycopeptide by destroying beta-1, 4 glycosidic bond between N-acetylmuramic acid and N-acetylglucosamine in the cell wall, so that the content of the broken cell wall is escaped to dissolve the bacteria. Lysozyme can also be directly combined with virus protein with negative charge, and forms a complex with DNA, RNA and apoprotein to inactivate the virus. Because the enzyme is widely present in various tissues of human bodies, egg white of birds and poultry, tears, saliva, blood plasma, milk and other liquids of mammals, the safety of the lysozyme to the human body is very high. Can act on bacteria and viruses.
3. The traditional Chinese herbal medicines of folium artemisiae argyi, honeysuckle, dandelion, fructus forsythiae and liquorice with sterilization or bacteriostasis effects are used as compatibility, and the extract of the Chinese herbal medicines is used as the component of the traditional Chinese herbal medicine preparation, so that the content of the sterilization component in the Chinese herbal medicines is mainly improved.
4. The added nisin and calcium propionate are food additives, can effectively kill bacteria and fungi, and have good stability and high safety.
5. The chitosan is added into the traditional Chinese medicine extract, and the traditional Chinese medicine is effectively and successfully wrapped in the chitosan to form microspheres, so that the sustained and controlled release effect can be achieved in sterilization and disinfection.
Detailed Description
The present invention will be described in detail with reference to the following embodiments in order to make the aforementioned objects, features and advantages of the invention more comprehensible. In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention. This invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein, but rather should be construed as broadly as the present invention is capable of modification in various respects, all without departing from the spirit and scope of the present invention.
The preparation method of the biological disinfectant containing silver ion water is characterized in that the formula comprises the following components in percentage by mass: 10-40 parts of biological enzyme composition, 30-50 parts of traditional Chinese medicine extract chelating liquid, 1-5 parts of nisin, 1-5 parts of calcium propionate and 10-40 parts of silver ion water.
The disinfectant adopting the biological enzyme main body is mainly characterized in that a novel sterilization method developed aiming at the problems that a chemical disinfectant has large residue and is easy to cause accumulation and poisoning is added, and meanwhile, a traditional Chinese medicine extract, nisin, calcium propionate and other biological enzymes with sterilization effects are added to act on bacteria, fungi, viruses and other microorganisms together, so that the disinfectant has the characteristics of high safety, quick sterilization, wide sterilization range, no generation of pungent odor and other high-efficiency sterilization.
The preparation method of the biological disinfectant comprises the following steps:
1. preparing a biological enzyme composition: mixing lysozyme, carboxypeptidase, collagen hydrolase, catalase and glucose oxidase according to mass fraction, adding silver ion water, filtering, and taking filtrate for later use;
carboxypeptidase is zinc-containing metalloprotease, and is used in medicine for in vivo toxin degradation, in vitro inflammation diminishing and wound quick repair. In the food industry, the method can be used for preparing high F value oligopeptides, removing ochratoxin in foods and feeds, and removing bitter agents.
The collagen hydrolase is used for hydrolyzing collagen, breaking the collagen and hydrolyzing the collagen by other proteases, does not hydrolyze fibrin, and can be used for treating burn, ulcer, skin ulcer and vertebral cartilage hyperplasia.
The biological function of the catalase is to promote the decomposition of hydrogen peroxide in cells, so that the hydrogen peroxide can not generate hydroxyl radical with great toxicity, thereby protecting the function of an antioxidant substance system, and having important significance for the growth and development and metabolic activities of human bodies.
The glucose oxidase is commonly called as detoxification enzyme, has an antioxidant effect, can remove free radicals generated by the metabolism of the intestinal tract of an organism, and protects the intestinal tract tissues from the oxidative damage of the free radicals, so that the intestinal tract epithelial cells are protected to be complete, the chances of coccidian and pathogenic bacteria invading the intestinal tract epithelial parasitic part are reduced, and the aims of defending the invasion of the coccidian and the pathogenic bacteria are achieved.
Through a large number of researches, the combined and compounded use of the enzymes can well kill or inhibit the activity of viruses and bacteria and prevent the propagation of the viruses and the bacteria, thereby having the effect of biological bacteriostasis.
2. Traditional Chinese medicine extraction: mixing folium Artemisiae Argyi, flos Lonicerae, herba Taraxaci, fructus forsythiae, and Glycyrrhrizae radix according to mass fraction, adding 95% ethanol, extracting for 3 times, each for 1 hr, mixing extractive solutions, cooling, filtering, adding decolorizing agent for decolorizing, recovering solvent, concentrating to density of 1.3g/ml, and naming as extractive solution I;
folium artemisiae argyi: chinese herbal medicine for preventing pestilence has been used for a long time in China, and modern medical research shows that essential oil components (fragrance components) in folium artemisiae argyi have inhibiting or killing effects on various pathogenic bacteria and viruses. The pneumococcus and the influenza bacillus have killing effect, and have inhibiting effect on typhoid fever, paratyphoid bacillus, tubercle bacillus, various skin pathogenic fungi and the like. Especially has the inhibiting effect on influenza virus, adenovirus, rhinovirus, mumps virus and herpes virus, and can obviously reduce the incidence rate of influenza by using the medicine to disinfect air. Meanwhile, the Chinese mugwort leaf has the effects of promoting healing and recovering for various diseases such as pyogenic inflammation, trauma, burn and scald infection, skin pyogenic infection, psoriasis, herpes zoster, upper respiratory infection and the like, and shows that the Chinese mugwort leaf has the effects of preventing diseases and protecting health and recovering.
Honeysuckle flower: the honeysuckle contains chlorogenic acid, luteolin glycoside and other pharmacological active ingredients, and has strong inhibiting effect on hemolytic streptococcus, staphylococcus aureus and other pathogenic bacteria, upper respiratory infection pathogenic virus and the like.
Dandelion: the alcohol extract of dandelion has strong bactericidal effect on staphylococcus aureus drug-resistant strains and hemolytic streptococcus. It also has antibacterial effect on Diplococcus pneumoniae, meningococcus, diphtheria bacillus, Pseudomonas aeruginosa, Shigella dysenteriae, typhoid bacillus, etc., and on Catalpa coccus.
Fructus forsythiae: the alcoholic extract of fructus forsythiae contains phillyrin and phillyrin phenols, and can inhibit typhoid bacillus, paratyphoid bacillus, Escherichia coli, dysentery bacillus, diphtheria bacillus, vibrio cholerae, staphylococcus, streptococcus, etc. Especially has strong inhibiting effect on dysentery bacillus and staphylococcus aureus. The fructus forsythiae extract has antibacterial, antiviral, antiinflammatory, radiation injury resisting, and antipyretic effects.
Licorice root: the licorice extract saponin compound has the activities of resisting inflammation, bacteria, virus, etc.
The purpose of extracting by adopting high-concentration alcohol in the step is to firstly extract fat-soluble volatile components in the Chinese herbal medicines, such as volatile oil in fructus forsythiae and folium artemisiae argyi, which have strong antibacterial activity, but the antibacterial activity mainly comes from the fat-soluble components, so that ethanol is firstly adopted for extracting to obtain the fat-soluble extract.
3. Adding 3 times of water into the dregs of a decoction extracted in the step (2), extracting for 3 times at 90 ℃ for 1 hour each time, combining the extracting solutions, cooling, filtering, adding activated carbon with 2-5 per mill of mass fraction of the extracting solution for decoloring, and naming the activated carbon as an extracting solution II;
the method adopts ethanol extraction and then water extraction of the dregs of a decoction, and the medicinal materials have some polysaccharide, saponin and flavonoid substances with good physiological activities such as antibiosis, antivirus and the like, and the polysaccharides, saponin and flavonoid substances cannot be extracted by a fat-soluble solvent, so the water-soluble solvent extraction is adopted, and the purpose of extracting by the fat-soluble solvent is mainly to protect volatile active ingredients so as to avoid great loss of the volatile ingredients after direct water extraction.
4. Mixing the extracting solution I and the extracting solution II according to the mass fraction of 7:3 to obtain a traditional Chinese medicine extract;
after the fat-soluble and water-soluble components are effectively mixed, the bacteriostasis is more perfect, and the components which are beneficial to resisting bacteria and viruses in the Chinese herbal medicine are completely reserved and utilized.
5. Adding chitosan with deacetylation degree of 90% in an amount of 2% by mass into the Chinese medicinal extract, chelating with the Chinese medicinal extract for 1 hr at 60 deg.C, centrifuging, and collecting supernatant to obtain Chinese medicinal extract chelating solution; (ii) a
Chitosan in the traditional Chinese medicine extract has three functions: firstly, the function of clarifying the traditional Chinese medicine extract can be achieved, macromolecular proteins and the like in the traditional Chinese medicine extract are precipitated by adding chitosan, and are removed by filtration, so that the content of effective components of the traditional Chinese medicine extract is increased; secondly, the chitosan has stronger bactericidal action, and after the chitosan is added, the chitosan and the traditional Chinese medicine extract are chelated to obtain the residual chitosan, silver ion water, biological enzyme, calcium propionate and nisin which are used for sterilization together; thirdly, the chitosan and the traditional Chinese medicine can wrap the traditional Chinese medicine extract to form microspheres in a certain temperature and time, and the sustained-release preparation is prepared, so that the disinfection and sterilization time is longer.
6. Mixing the biological enzyme composition, the Chinese medicinal extract chelating solution, nisin, calcium propionate and silver ion water according to mass fraction, adjusting the pH value to 6.5-7.5, stirring and filling to obtain the biological disinfectant containing silver ion water. When the biological disinfectant containing silver ion water is used, the stock solution is diluted by 100 times.
Nisin is a food additive produced by fermentation, is an antimicrobial peptide, has a strong inhibitory effect on spores of bacillus, and is useful for the growth and reproduction of many gram-positive bacteria such as clostridium botulinum, staphylococcus aureus, streptococcus hemolyticus, listeria, and clostridium stearothermophilus. The antibacterial effect of nisin is to cause cell membrane penetration, nutrient loss and membrane potential drop by interfering with the normal function of the cellular v-membrane, leading to the death of pathogenic and putrefying bacterial cells.
The calcium propionate is a food additive, has strong inhibiting effect on mould, gram-negative bacillus or aerobic bacillus, and can also inhibit the production of aflatoxin.
The scheme is that silver ion water, microbial metabolites such as biological enzyme, nisin, Chinese herbal medicine extracts and chemically synthesized antibacterial agent calcium propionate are completely combined through a scientific experimental formula to play a role respectively, and the antibacterial agent is a safe and efficient biological bacteriostatic agent, does not generate irritant gas, is convenient to use, is low in price and is easy to obtain.
Example 1
The preparation method of the biological disinfectant containing silver ion water is characterized in that the formula comprises the following components in percentage by mass: 40 parts of biological enzyme composition, 43 parts of Chinese medicinal extract chelating liquid, 5 parts of nisin, 1 part of calcium propionate and 11 parts of silver ion water.
The preparation method of the biological disinfectant comprises the following steps:
1. preparing a biological enzyme composition: firstly, 5 parts of lysozyme, 1 part of carboxypeptidase, 1 part of collagen hydrolase, 1 part of catalase and 2 parts of glucose oxidase are mixed, silver ion water with the amount of 3 times of the mass of the biological enzyme composition is added, and the filtrate is obtained for later use after filtration;
2. traditional Chinese medicine extraction: mixing 3 parts of folium artemisiae argyi, 4 parts of honeysuckle, 1 part of dandelion, 1 part of fructus forsythiae and 1 part of liquorice, adding 95% ethanol, extracting for 3 times, each time for 1 hour, combining extracting solutions, cooling, filtering, adding activated carbon with 2 thousandth of mass fraction of the extracting solution for decoloring, recovering a solvent, concentrating until the density is 1.3g/ml, and naming the extracting solution as extracting solution I;
3. adding 3 times of water into the dregs of a decoction extracted in the step (2), extracting for 3 times at 90 ℃ for 1 hour each time, combining the extracting solutions, cooling, filtering, adding activated carbon with 5 per mill of the mass fraction of the extracting solution for decoloring, and naming the extracting solution as extracting solution II;
4. mixing the extracting solution I and the extracting solution II according to the mass fraction of 7:3 to obtain a traditional Chinese medicine extract;
5. adding chitosan with deacetylation degree of 90% in an amount of 2% by mass into the Chinese medicinal extract, chelating with the Chinese medicinal extract for 1 hr at 60 deg.C, centrifuging, and collecting supernatant to obtain Chinese medicinal extract chelating solution;
6. mixing and stirring 40 parts of biological enzyme composition, 43 parts of Chinese medicinal extract chelating liquid, 5 parts of nisin, 1 part of calcium propionate and 11 parts of silver ion water according to mass fraction for 30 min. Adjusting the pH value to 6.5, and filling to obtain the biological disinfectant containing silver ion water, wherein the stock solution of the biological disinfectant containing silver ion water is diluted by 100 times for use.
Example 2
A preparation method of a biological disinfectant containing silver ion water comprises the following components in parts by mass: 20 parts of biological enzyme composition, 30 parts of Chinese medicinal extract chelating liquid, 5 parts of nisin, 5 parts of calcium propionate and 40 parts of silver ion water.
The preparation method of the biological disinfectant comprises the following steps:
1. preparing a biological enzyme composition: mixing 3 parts of lysozyme, 3 parts of carboxypeptidase, 1 part of collagen hydrolase, 1 part of catalase and 2 parts of glucose oxidase, adding silver ion water which is 5 times of the amount of the biological enzyme composition, filtering, and taking filtrate for later use;
2. traditional Chinese medicine extraction: mixing 5 parts of folium artemisiae argyi, 1 part of honeysuckle, 1 part of dandelion, 1 part of fructus forsythiae and 2 parts of liquorice, adding 95% ethanol, extracting for 3 times, each time for 1 hour, combining extracting solutions, cooling, filtering, adding activated carbon with 2 thousandth of mass fraction of the extracting solution for decoloring, recovering a solvent, concentrating until the density is 1.3g/ml, and naming the extracting solution as extracting solution I;
3. adding 3 times of water into the dregs of a decoction extracted in the step (2), extracting for 3 times at 90 ℃ for 1 hour each time, combining the extracting solutions, cooling, filtering, adding activated carbon with 5 per mill of the mass fraction of the extracting solution for decoloring, and naming the extracting solution as extracting solution II;
4. mixing the extracting solution I and the extracting solution II according to the mass fraction of 7:3 to obtain a traditional Chinese medicine extract;
5. adding chitosan with deacetylation degree of 90% in an amount of 2% by mass into the Chinese medicinal extract, chelating with the Chinese medicinal extract for 1 hr at 60 deg.C, centrifuging, and collecting supernatant to obtain Chinese medicinal extract chelating solution;
6. mixing and stirring 20 parts of biological enzyme composition, 30 parts of Chinese medicinal extract chelating liquid, 5 parts of nisin, 5 parts of calcium propionate and 40 parts of silver ion water according to mass fraction for 30 min. Adjusting the pH value to 7.0, and filling to obtain the biological disinfectant containing silver ion water, wherein the stock solution of the biological disinfectant containing silver ion water is diluted by 100 times for use.
Example 3
A preparation method of a biological disinfectant containing silver ion water comprises the following components in parts by mass: 20 parts of biological enzyme composition, 50 parts of Chinese medicinal extract chelating liquid, 1 part of nisin, 1 part of calcium propionate and 28 parts of silver ion water.
The preparation method of the biological disinfectant comprises the following steps:
1. preparing a biological enzyme composition: mixing 4 parts of lysozyme, 2 parts of carboxypeptidase, 2 parts of collagen hydrolase, 1 part of catalase and 1 part of glucose oxidase, adding silver ion water in an amount which is 4 times that of a biological enzyme composition, filtering, and taking filtrate for later use;
2. traditional Chinese medicine extraction: mixing 4 parts of folium artemisiae argyi, 2 parts of honeysuckle, 1 part of dandelion, 2 parts of fructus forsythiae and 1 part of liquorice, adding 95% ethanol, extracting for 3 times, each time for 1 hour, combining the extracting solutions, cooling, filtering, adding activated carbon with the mass fraction of 3 per thousand of the filtrate for decoloring, recovering the solvent, concentrating until the density is 1.3g/ml, and naming the extracting solution as extracting solution I;
3. adding 3 times of water into the dregs of a decoction extracted in the step (2), extracting for 3 times at 90 ℃ for 1 hour each time, combining the extracting solutions, cooling, filtering, adding activated carbon with the mass fraction of 2 per mill of the filtrate for decoloring, and naming the mixture as extracting solution II;
4. mixing the extracting solution I and the extracting solution II according to the mass fraction of 7:3 to obtain a traditional Chinese medicine extract;
5. adding chitosan with deacetylation degree of 90% in an amount of 2% by mass into the Chinese medicinal extract, chelating with the Chinese medicinal extract for 1 hr at 60 deg.C, centrifuging, and collecting supernatant to obtain Chinese medicinal extract chelating solution;
6. mixing and stirring 20 parts of biological enzyme composition, 50 parts of Chinese medicinal extract chelating liquid, 1 part of nisin, 1 part of calcium propionate and 28 parts of silver ion water according to mass fraction for 30 min. Adjusting the pH value to 7.5, and filling to obtain the biological disinfectant containing silver ion water, wherein the stock solution of the biological disinfectant containing silver ion water is diluted by 100 times for use.
Example 4
A preparation method of a biological disinfectant containing silver ion water comprises the following components in parts by mass: 30 parts of biological enzyme composition, 30 parts of Chinese medicinal extract chelating liquid, 5 parts of nisin, 5 parts of calcium propionate and 30 parts of silver ion water.
The preparation method of the biological disinfectant comprises the following steps:
1. preparing a biological enzyme composition: mixing 3 parts of lysozyme, 3 parts of carboxypeptidase, 1 part of collagen hydrolase, 1 part of catalase and 23 parts of glucose oxidase, adding silver ion water which is 5 times of the amount of the biological enzyme composition, filtering, and taking filtrate for later use;
2. traditional Chinese medicine extraction: mixing 5 parts of folium artemisiae argyi, 1 part of honeysuckle, 1 part of dandelion, 2 parts of fructus forsythiae and 1 part of liquorice, adding 95% ethanol, extracting for 3 times, each time for 1 hour, combining the extracting solutions, cooling, filtering, adding 2 per mill of activated carbon of the mass fraction of the filtrate for decoloring, recovering the solvent, concentrating until the density is 1.3g/ml, and naming as extracting solution I;
3. adding 3 times of water into the dregs of a decoction extracted in the step (2), extracting for 3 times at 90 ℃ for 1 hour each time, combining the extracting solutions, cooling, filtering, adding 2 per mill of activated carbon of the mass fraction of the filtrate for decoloring, and naming the mixture as extracting solution II;
4. mixing the extracting solution I and the extracting solution II according to the mass fraction of 7:3 to obtain a traditional Chinese medicine extract;
5. adding chitosan with deacetylation degree of 90% in an amount of 2% by mass into the Chinese medicinal extract, chelating with the Chinese medicinal extract for 1 hr at 60 deg.C, centrifuging, and collecting supernatant to obtain Chinese medicinal extract chelating solution;
6. mixing and stirring 30 parts of biological enzyme composition, 30 parts of Chinese medicinal extract chelating liquid, 5 parts of nisin, 5 parts of calcium propionate and 30 parts of silver ion water according to mass fraction for 30 min. Adjusting the pH value to 6.5, and filling to obtain the biological disinfectant containing silver ion water, wherein the stock solution of the biological disinfectant containing silver ion water is diluted by 100 times for use.
Comparative example 1
A preparation method of a biological disinfectant containing silver ion water comprises the following components in parts by mass: 40 parts of biological enzyme composition, 5 parts of nisin, 1 part of calcium propionate and 54 parts of silver ion water.
The preparation method of the biological disinfectant containing silver ion water comprises the following steps:
1. preparing a biological enzyme composition: mixing 5 parts of lysozyme, 1 part of carboxypeptidase, 1 part of collagen hydrolase, 1 part of catalase and 2 parts of glucose oxidase, adding silver ion water in an amount which is 3 times that of a biological enzyme composition, and filtering to obtain filtrate for later use;
2. mixing and stirring 40 parts of biological enzyme composition, 5 parts of nisin, 1 part of calcium propionate and 54 parts of silver ion water according to mass fraction for 30 min. Adjusting the pH value to 6.5, and filling to obtain the biological disinfectant containing silver ion water, wherein the stock solution of the biological disinfectant containing silver ion water is diluted by 100 times for use.
Comparative example 2
The formula of the biological disinfectant comprises the following components in parts by mass: 40 parts of biological enzyme composition, 43 parts of traditional Chinese medicine composition, 1 part of chitosan, 5 parts of nisin, 1 part of calcium propionate and 10 parts of distilled water.
The preparation method of the biological disinfectant comprises the following steps:
1. preparing a biological enzyme composition: mixing 5 parts of lysozyme, 1 part of carboxypeptidase, 1 part of collagen hydrolase, 1 part of catalase and 2 parts of glucose oxidase, adding 3 times of normal-temperature distilled water of a biological enzyme composition, and filtering to obtain filtrate for later use;
2. extracting the traditional Chinese medicine composition: mixing 3 parts of folium artemisiae argyi, 4 parts of honeysuckle, 1 part of dandelion, 1 part of fructus forsythiae and 1 part of liquorice, adding 95% ethanol, extracting for 3 times, each time for 1 hour, combining extracting solutions, cooling, filtering, adding activated carbon with the mass fraction of 2 thousandth of the extracting solution for decoloring, recovering a solvent, and concentrating to the density of 1.3g/ml to obtain a traditional Chinese medicine composition;
3. mixing and stirring 40 parts of biological enzyme composition, 43 parts of traditional Chinese medicine composition extract, 1 part of chitosan, 5 parts of nisin, 1 part of calcium propionate and 10 parts of distilled water according to mass fraction for 30 min. Adjusting pH to 6.5, and bottling to obtain biological disinfectant, wherein the biological disinfectant is used by diluting the stock solution by 100 times.
Comparative example 3
The formula of the biological disinfectant comprises the following components in parts by mass: 40 parts of biological enzyme composition, 43 parts of traditional Chinese medicine composition extract, 1 part of chitosan, 5 parts of nisin, 1 part of calcium propionate and 10 parts of distilled water.
The preparation method of the biological disinfectant comprises the following steps:
1. preparing a biological enzyme composition: mixing 5 parts of lysozyme, 1 part of carboxypeptidase, 1 part of collagen hydrolase, 1 part of catalase and 2 parts of glucose oxidase, adding 3 times of normal-temperature distilled water of a biological enzyme composition, and filtering to obtain filtrate for later use;
2. extracting the traditional Chinese medicine composition: mixing 3 parts of folium artemisiae argyi, 4 parts of honeysuckle, 1 part of dandelion, 1 part of fructus forsythiae and 1 part of liquorice, adding water, extracting for 3 times, each time for 1 hour, combining the extracting solutions, cooling, filtering, adding activated carbon with the mass fraction of 2 thousandth of the extracting solution for decoloring, recovering the solvent, and concentrating to the density of 1.2g/ml to obtain the traditional Chinese medicine composition extract;
5. mixing and stirring 40 parts of biological enzyme composition, 43 parts of traditional Chinese medicine composition extract, 1 part of chitosan, 5 parts of nisin, 1 part of calcium propionate and 10 parts of distilled water according to mass fraction for 30 min. Adjusting pH to 6.5, and bottling to obtain biological disinfectant, wherein the biological disinfectant is used by diluting the stock solution by 100 times.
Comparative example 4
The formula of the biological disinfectant comprises the following components in parts by mass: 40 parts of biological enzyme composition, 43 parts of traditional Chinese medicine composition extract and 17 parts of distilled water.
The preparation method of the biological disinfectant comprises the following steps:
1. preparing a biological enzyme composition: mixing 5 parts of lysozyme, 1 part of carboxypeptidase, 1 part of collagen hydrolase, 1 part of catalase and 2 parts of glucose oxidase, adding 3 times of normal-temperature distilled water of a biological enzyme composition, and filtering to obtain filtrate for later use;
2. extracting the traditional Chinese medicine composition: mixing 3 parts of folium artemisiae argyi, 4 parts of honeysuckle, 1 part of dandelion, 1 part of fructus forsythiae and 1 part of liquorice, adding 95% ethanol, extracting for 3 times, each time for 1 hour, combining extracting solutions, cooling, filtering, adding activated carbon with 2 thousandth of mass fraction of the extracting solution for decoloring, recovering a solvent, concentrating until the density is 1.3g/ml, and naming the extracting solution as extracting solution I;
3. adding 3 times of water into the dregs of a decoction extracted in the step (2), extracting for 3 times at 90 ℃ for 1 hour each time, combining the extracting solutions, cooling, filtering, adding activated carbon with 5 per mill of the mass fraction of the extracting solution for decoloring, recovering the solvent, and concentrating to the density of 1.2g/ml, which is named as extracting solution II;
4. mixing the extracting solution I and the extracting solution II according to the mass fraction of 7:3 to obtain a traditional Chinese medicine composition extract;
5. mixing and stirring 40 parts of biological enzyme composition, 43 parts of traditional Chinese medicine composition and 17 parts of distilled water according to mass fraction for 30 min. Adjusting pH to 6.5, and bottling to obtain biological disinfectant, wherein the biological disinfectant is used by diluting the stock solution by 100 times.
Comparative example 5
The formula of the biological disinfectant comprises the following components in parts by mass: 43 parts of traditional Chinese medicine composition extract, 1 part of chitosan, 5 parts of nisin, 1 part of calcium propionate and 50 parts of distilled water.
A preparation method of the biological disinfectant comprises the following steps:
1. extracting the traditional Chinese medicine composition: mixing 3 parts of folium artemisiae argyi, 4 parts of honeysuckle, 1 part of dandelion, 1 part of fructus forsythiae and 1 part of liquorice, adding 95% ethanol, extracting for 3 times, each time for 1 hour, combining extracting solutions, cooling, filtering, adding activated carbon with 2 thousandth of mass fraction of the extracting solution for decoloring, recovering a solvent, concentrating until the density is 1.3g/ml, and naming the extracting solution as extracting solution I;
2. adding 3 times of water into the dregs of a decoction extracted in the step (2), extracting for 3 times at 90 ℃ for 1 hour each time, combining the extracting solutions, cooling, filtering, adding activated carbon with 5 per mill of the mass fraction of the extracting solution for decoloring, recovering the solvent, and concentrating to the density of 1.2g/ml, which is named as extracting solution II;
3. mixing the extracting solution I and the extracting solution II according to the mass fraction of 7:3 to obtain a traditional Chinese medicine composition extract;
4. mixing 43 parts of the traditional Chinese medicine composition extract, 1 part of chitosan, 5 parts of nisin, 1 part of calcium propionate and 50 parts of distilled water according to mass fraction, and stirring for 30 min. Adjusting pH to 6.5, and bottling to obtain biological disinfectant, wherein the disinfectant is used by diluting stock solution by 100 times.
Sterilization test
Inoculating Escherichia coli, Staphylococcus aureus, Candida albicans, Bacillus subtilis, Aspergillus niger, yeast, rhinovirus, and adenovirus in culture medium, and culturing for 20 hr. Washing thallus Porphyrae in the culture medium with sterile physiological saline, adding 0.5mL of experimental bacterial suspension into sterile test tube with 30g/L bovine serum albumin, mixing, keeping temperature to 20 + -1 deg.C in water bath, adding 10mL of disinfectant (0.1mL of the inventive disinfectant is diluted to 10mL with sterile water) into the test tube, mixing, and timing. Sucking 0.5mL of mixed liquid of the bacterial suspension and the disinfectant prepared in the embodiment 1 of the invention, mixing the mixed liquid with 4.5mL of neutralizing agent TSB for 5-10min, sucking 1mL of mixed liquid, measuring the number of viable bacteria according to a viable bacteria culture counting method, inoculating 3 plates in each tube, counting the number of viable bacteria, calculating the killing rate, and unifying the sterilization time to 5 min. The results are shown in Table 1:
TABLE 1 Sterilization test
As can be seen from Table 1: the sterilization formula has the advantages that the sterilization formula is well optimized, and the water-soluble and fat-soluble components of the biological enzyme and the Chinese herbal medicine extract, the chitosan, the nisin and the calcium propionate are scientifically matched, so that the product has a good effect on various harmful bacteria.
Product stability test
As the product is a biological disinfectant containing silver ion water and the biological enzyme is an active substance, the product and the comparative example are mainly considered to be placed for 60 days at 0-40 ℃, the stability of the product is considered from the aspects of appearance and enzyme activity measurement, and the enzyme activity measurement of the product is determined according to the method of food additive lysozyme of GB1886.257-2016 food safety national standard.
2 parts of the products of the examples 1 to 4 and the products of the comparative examples 1 to 4 are respectively taken and placed at 0 ℃, 20 ℃ and 40 ℃ for 60 days, the appearance change of the products is observed, the products are taken out after being placed for 60 days to determine the activity of lysozyme, and the activity of the lysozyme added in the biological disinfectant containing silver ion water is not lower than 1000 u/ml.
TABLE 2 product stability test
0 deg.C (60 days) 10 deg.C (60 days) 20 deg.C (60 days) 30 ℃ (60 days) 40 deg.C (60 days)
Example 1 Not delaminating Not delaminating Not delaminating Not delaminating Not delaminating
Example 2 Not delaminating Not delaminating Not delaminating Not delaminating Not delaminating
Example 3 Not delaminating Not delaminating Not delaminating Not delaminating Not delaminating
Example 4 Not delaminating Not delaminating Not delaminating Not delaminating Not delaminating
Comparative example 1 Not delaminating Not delaminating Not delaminating Has been layered Has been layered
Comparative example 2 Not delaminating Has been layered Has been layered Has been layered Has been layered
Comparative example 3 Not delaminating Not delaminating Not delaminating Has been layered Has been layered
Comparative example 4 Not delaminating Not delaminating Not delaminating Has been layered Has been layered
Comparative example 5 Not delaminating Not delaminating Has been layered Has been layered Has been layered
From table 2, it can be seen that: the examples 1-4 are respectively placed at 0-40 ℃ for 60 days, the appearance is not changed, which shows that the stability is good, the comparative examples 1-5 respectively have the phenomenon of layering at 10-40 ℃, which shows that the quality is unstable and the quality guarantee period is short, and the non-layering of the examples is mainly because the chitosan and the fat-soluble traditional Chinese medicine extract are added, the emulsification effect is realized in the formula, and the quality guarantee period is greatly prolonged.
TABLE 3 measurement of lysozyme activity
Figure BDA0002444745190000121
Figure BDA0002444745190000131
As can be seen from Table 3, the lysozyme activity changes greatly after being placed at 0-40 ℃ for 60 days, the lysozyme activity hardly changes after being placed at 40 ℃ for 60 days in the embodiment, but the lysozyme is almost lost almost in the comparative examples 1-4 after being placed at 40 ℃ for 60 days, which shows that the product has a very good enzyme activity protection effect, and the product has good stability and can be stably stored for 60 days.
The above-mentioned embodiments only express several embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the present invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention. Therefore, the protection scope of the present patent shall be subject to the appended claims.

Claims (10)

1. The preparation method of the biological disinfectant containing silver ion water is characterized in that the formula comprises the following components in percentage by mass: 10-40 parts of biological enzyme composition, 30-50 parts of traditional Chinese medicine extract chelating liquid, 1-5 parts of nisin, 1-5 parts of calcium propionate and 10-40 parts of silver ion water.
2. The method for preparing a biological disinfectant containing silver ion water according to claim 1, wherein said biological enzyme composition comprises, in mass fraction: 3-5 parts of lysozyme, 1-3 parts of carboxypeptidase, 1-3 parts of collagen hydrolase, 1-3 parts of catalase and 1-3 parts of glucose oxidase.
3. The method for preparing biological disinfectant containing silver ion water according to claim 1, wherein the traditional Chinese medicine comprises the following components by mass percent: 3-5 parts of folium artemisiae argyi, 1-4 parts of honeysuckle, 1-3 parts of dandelion, 1-3 parts of fructus forsythiae and 1-3 parts of liquorice.
4. The method for preparing a biological disinfectant containing silver ion water according to claim 1, wherein the silver ion water is water prepared by electrolyzing silver ions, has a sterilizing effect, and has a silver ion content of 50-80 ppm.
5. The method of preparing a biological disinfectant from biological silver-ion-containing water according to claim 1, wherein:
(1) preparing a biological enzyme composition: mixing lysozyme, carboxypeptidase, collagen hydrolase, catalase and glucose oxidase according to mass percent, adding silver ion water, filtering, and taking filtrate for later use;
(2) traditional Chinese medicine extraction: mixing folium artemisiae argyi, honeysuckle, dandelion, fructus forsythiae and liquorice according to mass percentage, adding 95% ethanol, extracting for 3 times, extracting for 1 hour each time, combining the extracting solutions, cooling, filtering, adding a decolorizing agent for decolorizing, recovering the solvent, concentrating until the density is 1.3g/ml, and naming the extracting solution as extracting solution I;
(3) adding 3 times of water into the dregs of a decoction extracted in the step (2), extracting for 3 times at 90 ℃ for 1 hour each time, combining the extracting solutions, cooling, filtering, adding a decoloring agent for decoloring, and naming the extracting solution as an extracting solution II;
(4) mixing the extracting solution I and the extracting solution II according to the mass fraction to obtain a traditional Chinese medicine extract;
(5) adding chitosan with deacetylation degree of 90% in an amount of 2% by mass into the Chinese medicinal extract, chelating with the Chinese medicinal extract for 1 hr at 60 deg.C, centrifuging, and collecting supernatant to obtain Chinese medicinal extract chelating solution;
(6) mixing the biological enzyme composition, the Chinese medicinal extract chelating solution, nisin, calcium propionate and silver ion water according to mass fraction, adjusting the pH value to 6.5-7.5, stirring and filling to obtain the biological disinfectant containing silver ion water.
6. The method for preparing a biological disinfectant containing silver ion water according to claim 5, wherein the silver ion water in step (1) is added in an amount of 3-5 times the mass fraction of the biological enzyme composition.
7. The method for preparing biological disinfectant containing silver ion water according to claim 5, wherein the decolorizing agent in steps (2) and (3) is activated carbon, and the adding amount of the decolorizing agent is 2-5 per mill of the mass fraction of the extracting solution.
8. The method for preparing a biological disinfectant containing silver ion water according to claim 5, wherein in the step (4), the extract I and the extract II are mixed according to the mass fraction of 7: 3.
9. The method for preparing a biological disinfectant containing silver ion water according to claim 5, wherein the mixing and stirring time in step (5) is 30 min.
10. The method for preparing a biological disinfectant containing silver ion water according to claim 1, wherein the stock solution is diluted 100 times for use.
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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112210450A (en) * 2020-09-30 2021-01-12 广州洁宜日化用品有限公司 Antibacterial laundry detergent and preparation method and application thereof
CN115176820A (en) * 2022-07-08 2022-10-14 沈阳药科大学 Polygonum cuspidatum silver ion disinfectant and preparation method and application thereof

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112210450A (en) * 2020-09-30 2021-01-12 广州洁宜日化用品有限公司 Antibacterial laundry detergent and preparation method and application thereof
CN115176820A (en) * 2022-07-08 2022-10-14 沈阳药科大学 Polygonum cuspidatum silver ion disinfectant and preparation method and application thereof
CN115176820B (en) * 2022-07-08 2023-10-24 沈阳药科大学 Polygonum cuspidatum silver ion disinfectant, and preparation method and application thereof

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