CN111567180A - Method for improving saline-alkali resistance of forage grass seeds by utilizing smoke and water excitation - Google Patents
Method for improving saline-alkali resistance of forage grass seeds by utilizing smoke and water excitation Download PDFInfo
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- CN111567180A CN111567180A CN202010528810.XA CN202010528810A CN111567180A CN 111567180 A CN111567180 A CN 111567180A CN 202010528810 A CN202010528810 A CN 202010528810A CN 111567180 A CN111567180 A CN 111567180A
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Abstract
A method for improving the saline-alkali resistance of grass seeds by using smoke and water excitation relates to a method for improving the saline-alkali resistance of grass seeds by using smoke and water excitation. The invention aims to solve the technical problems of low germination rate, low emergence rate and low survival rate of the forage grass seeds under the saline-alkali condition. The method comprises the following steps: firstly, preparing a plant source tobacco water mother solution: and secondly, the tobacco water of the grass seeds is excited. The invention relates to a method for improving saline-alkali resistance of grass seeds by using smoke water excitation, which is a method for improving the germination rate of the grass seeds and the growth of seedlings under saline-alkali conditions by dissolving smoke generated by biomass combustion in water and exciting the grass seeds by using generated plant source smoke water. The invention belongs to the technical field of improving the germination rate of forage grass seeds under the saline-alkali condition.
Description
Technical Field
The invention relates to a method for improving saline-alkali resistance of grass seeds by utilizing smoke and water excitation.
Background
The herbage seed germination rate is low, the emergence rate is low and the survival rate is low under the saline-alkali condition, the smoke generated by biomass combustion is generated along with naked flame, a certain duration exists before and after the naked flame occurs, a large amount of volatile organic matters are contained in the smoke, the substances inevitably influence the growth of a burned area and surrounding plants, and smoke water solution generated by rainfall after the fire, thermal shock effect generated by combustion and the like all have important influence on the composition elements of an ecosystem. As the chemical substances in the tobacco for promoting the germination of the seeds are deposited on the soil surface, the seedlings can be promoted to germinate from the soil seed bank after the fire.
Disclosure of Invention
The invention aims to solve the technical problems of low germination rate, low emergence rate and low survival rate of grass seeds under the saline-alkali condition, and provides a method for improving saline-alkali resistance of the grass seeds by utilizing smoke and water excitation.
The method for improving the saline-alkali resistance of the grass seeds by utilizing the stimulation of the smoke and water is carried out according to the following steps:
firstly, preparing a plant source tobacco water mother solution:
putting dried green plant leaves into a combustion box connected with an air blower, combusting for 30 minutes, introducing a tobacco hose generated after combustion into a container filled with 1 liter of water, and cooling to obtain a plant-derived tobacco water mother liquor;
secondly, the tobacco water excitation of the grass seeds:
diluting the mass concentration of the plant source tobacco mother liquor to 1.5-3.0%, pouring the diluted solution into a centrifugal tube of 50-100 ml, putting the grass seeds into a nylon mesh bag, putting the nylon mesh bag filled with the seeds into the centrifugal tube, completely immersing the nylon mesh bag in the plant source tobacco mother liquor, screwing the cover of the centrifugal tube, putting the centrifugal tube on an oscillator, and oscillating the centrifugal tube at the speed of 100 revolutions per minute for 20-24 hours to finish the method for improving the saline-alkali resistance of the grass seeds by utilizing the excitation of the tobacco water.
And in the step one, the green plant leaves are green and fresh leaves of poplar and are dried at room temperature.
The method for improving the saline-alkali resistance of the grass seeds by using the smoke water excitation has the advantages that the tobacco generated by burning the biomass is dissolved in water, the generated plant source smoke water is used for exciting the grass seeds, and the germination rate and seedling growth of the grass seeds under the saline-alkali condition are improved. Therefore, the method aims to stimulate the grass seeds through the plant source tobacco water, so that the germination rate and the seedling growth of the grass seeds under the saline-alkali condition are improved, and a novel method which is simple, efficient, easy to operate and low in cost is provided for vegetation recovery in saline-alkali soil and field planting of high-quality grass under the saline-alkali condition.
Drawings
FIG. 1 is a graph showing the growth of the young roots of Saxifraga truncata under the stress of NaCl at a concentration of 0mmol/L in experiment one;
FIG. 2 is a graph showing the growth of the young roots of Saxifraga truncata under the stress of NaCl at a concentration of 25mmol/L in experiment one;
FIG. 3 is a graph showing the growth of the young roots of Saxifraga truncata under the stress of NaCl at a concentration of 50mmol/L in experiment one;
FIG. 4 is a graph showing the growth of the young roots of Saxifraga truncata under NaCl stress at a concentration of 100mmol/L in experiment one.
Detailed Description
The technical solution of the present invention is not limited to the following specific embodiments, but includes any combination of the specific embodiments.
The first embodiment is as follows: the method for improving the saline-alkali resistance of the grass seeds by utilizing the smoke and water excitation is carried out according to the following steps:
firstly, preparing a plant source tobacco water mother solution:
putting dried green plant leaves into a combustion box connected with an air blower, combusting for 30 minutes, introducing a tobacco hose generated after combustion into a container filled with 1 liter of water, and cooling to obtain a plant source tobacco water mother liquor;
secondly, the tobacco water excitation of the grass seeds:
diluting the mass concentration of the plant source tobacco mother liquor to 1.5-3.0%, pouring the diluted solution into a centrifugal tube of 50-100 ml, putting the grass seeds into a nylon mesh bag, putting the nylon mesh bag filled with the seeds into the centrifugal tube, completely immersing the nylon mesh bag in the plant source tobacco mother liquor, screwing the cover of the centrifugal tube, putting the centrifugal tube on an oscillator, and oscillating the centrifugal tube at the speed of 100 revolutions per minute for 20-24 hours to finish the method for improving the saline-alkali resistance of the grass seeds by utilizing the excitation of the tobacco water.
The second embodiment is as follows: the difference between the first embodiment and the second embodiment is that the green plant leaves in the first step are fresh leaves of poplar, and the leaves are dried at room temperature. The rest is the same as the first embodiment.
The third concrete implementation mode: the present embodiment is different from the first or second embodiment in that the plant-derived tobacco mother liquor is diluted to 1.8% by mass in the second step. The others are the same as in the first or second embodiment.
The fourth concrete implementation mode: the present embodiment is different from the first to third embodiments in that the plant-derived tobacco mother liquor is diluted to 2.0% by mass in the second step. The rest is the same as one of the first to third embodiments.
The fifth concrete implementation mode: the present embodiment is different from the first to the fourth embodiments in that the plant-derived tobacco mother liquor is diluted to 2.5% by mass in the second step. The rest is the same as one of the first to fourth embodiments.
The sixth specific implementation mode: the present embodiment is different from the first to fifth embodiments in that the plant-derived tobacco mother liquor is diluted to 3% by mass in the second step. The rest is the same as one of the first to fifth embodiments.
The seventh embodiment: the difference between this embodiment and the first to sixth embodiments is that in the second step, the oscillation is performed at a speed of 100 rpm for 20 hours. The rest is the same as one of the first to sixth embodiments.
The specific implementation mode is eight: the difference between this embodiment and the first to seventh embodiments is that in the second step, the oscillation is performed at a speed of 100 rpm for 21 hours. The rest is the same as one of the first to seventh embodiments.
The specific implementation method nine: the difference between this embodiment and the first to eighth embodiments is that in the second step, the oscillation is performed at a speed of 100 rpm for 22 hours. The rest is the same as the first to eighth embodiments.
The detailed implementation mode is ten: the difference between this embodiment and one of the first to ninth embodiments is that in the second step, the oscillation is performed at a speed of 100 rpm for 23 hours. The rest is the same as one of the first to ninth embodiments.
The following experiments are adopted to verify the effect of the invention:
experiment one:
the experiment is carried out in 2019 in 10-12 months, takes perennial saxifraga truncata (Chloristrunca) seeds of Saxifraga of Gramineae as research objects, and uses smoke water to excite the method for improving the saline-alkali resistance of the grass seeds according to the following steps:
firstly, preparing a plant source tobacco water mother solution:
putting dried green plant leaves into a combustion box connected with an air blower, combusting for 30 minutes, introducing a tobacco hose generated after combustion into a container filled with 1 liter of water, and cooling to obtain a plant source tobacco water mother liquor;
secondly, the tobacco water excitation of the grass seeds:
diluting the mass concentration of the plant source tobacco mother liquor to 1.5-3.0%, pouring the diluted solution into a centrifugal tube of 50-100 ml, putting the saxifrage truncatum into a nylon mesh bag, putting the nylon mesh bag filled with the saxifrage truncatum into the centrifugal tube, completely immersing the nylon mesh bag in the plant source tobacco mother liquor, screwing down the cover of the centrifugal tube, putting the centrifugal tube on an oscillator, and oscillating the nylon mesh bag for 20-24 hours at the speed of 100 revolutions per minute, thus completing the method for improving the saline-alkali resistance of the grass seeds by utilizing the excitation of the tobacco water.
Thirdly, preparing a culture medium for seed germination:
weighing agar powder, dissolving in a triangular flask, adding water, sterilizing at 121 deg.C for 20 min in an autoclave, cooling to 80 deg.C, taking out, shaking, adding NaCl mother liquor with different amount and NaCl concentration gradient of 0 (control, no NaCl is added), 25mmol/L, 50mmol/L and 100mmol/L NaCl, pouring the culture medium into sterilized culture dish in sterilized clean bench, and cooling and solidifying.
Fourthly, sowing and germinating the pasture:
and (3) sowing the excited seeds of the saxifrage truncatum in the agar culture medium containing different NaCl, sowing 25 full seeds in each culture dish, and repeating each NaCl gradient for 4 times. The culture dish after seeding is sealed by a preservative film and is put into an incubator at 15/25 ℃.
And fifthly, investigating the germination rate of grass seeds and the growth of seedlings. And (4) investigating the germination rate of the seeds every day from the next day after sowing, wherein the germination is finished by taking the germination of the seeds which are not newly increased for three consecutive days as a standard. After the experiment, the root length and the seedling length of the seedling were measured with a vernier caliper.
The result shows that the germination rate of the saxifraga truncata seeds can be obviously improved by the excitation of the smoke and water under the NaCl salt and alkali with the concentration of 0-100 mmol/L.
The specific results are as follows:
under the condition without NaCl stress, the germination rate (82.1%) of the saxifrage truncatum after the tobacco water excitation is improved by 16.2% compared with that of the seeds without the excitation treatment (65.9%);
under the NaCl with the concentration of 25mmol/L, the germination rate (77.8%) of the saxifrage truncatum after the smoke water excitation is improved by 21.4% compared with the germination rate of the seeds without the excitation treatment (56.4%);
under the NaCl with the concentration of 50mmol/L, the germination rate (68.3%) of the saxifrage truncatum after the smoke and water excitation is improved by 33.2% compared with the germination rate of the seeds without the excitation treatment (35.1%);
under the NaCl with the concentration of 100mmol/L, the germination rate (54.7%) of the saxifrage truncatum after the smoke water excitation is improved by 37.8% compared with the germination rate of the seeds without the excitation treatment (16.9%), and the two types of seeds have obvious difference.
The growth of the young roots of the saxifrage truncatum under the stress of NaCl with the concentration of 0-100mmol/L is obviously improved by the excitation of the smoke water (figures 1-4), and compared with the non-excitation treatment under the stress of the same saline alkali, the root length is improved by 23.7-39.2% by the excitation treatment of the smoke water.
The specific results are as follows:
under the condition of no NaCl stress, the root length of the truncated saxifrage after the excitation of the tobacco water is 2.42 +/-0.08 cm, the root length of the truncated saxifrage after the excitation of the tobacco water is 1.78 +/-0.07 cm, and the root length is improved by 36.0 percent;
under the condition of NaCl with the concentration of 25mmol/L, the root length of the truncated saxifrage after the excitation of the smoke water is 2.77 +/-0.12 cm, the root length of the truncated saxifrage after the excitation of the smoke water is 1.99 +/-0.06 cm, and the root length is improved by 39.2 percent;
under the condition of NaCl with the concentration of 50mmol/L, the root length of the truncated saxifrage after the excitation of the smoke water is 2.61 +/-0.12 cm, the root length of the truncated saxifrage after the excitation of the smoke water is 2.11 +/-0.09 cm, and the improvement is 237 percent.
When the NaCl stress concentration is 100mmol/L, the growth of the young roots is not obviously promoted by the smoke excitation.
Claims (10)
1. The method for improving the saline-alkali resistance of the grass seeds by using the smoke and water excitation is characterized by comprising the following steps of:
firstly, preparing a plant source tobacco water mother solution:
putting dried green plant leaves into a combustion box connected with an air blower, combusting for 30 minutes, introducing a tobacco hose generated after combustion into a container filled with 1 liter of water, and cooling to obtain the plant-derived tobacco water mother liquor.
Secondly, the tobacco water excitation of the grass seeds:
diluting the mass concentration of the plant source tobacco mother liquor to 1.5-3.0%, pouring the diluted solution into a centrifugal tube of 50-100 ml, putting the grass seeds into a nylon mesh bag, putting the nylon mesh bag filled with the seeds into the centrifugal tube, completely immersing the nylon mesh bag in the plant source tobacco mother liquor, screwing the cover of the centrifugal tube, putting the centrifugal tube on an oscillator, and oscillating the centrifugal tube at the speed of 100 revolutions per minute for 20-24 hours to finish the method for improving the saline-alkali resistance of the grass seeds by utilizing the excitation of the tobacco water.
2. The method for improving saline-alkali resistance of grass seeds by using smoke and water excitation as claimed in claim 1, wherein the green plant leaves in the first step are fresh leaves of poplar and are dried at room temperature.
3. The method for improving the saline-alkali resistance of the grass seeds by using the smoke water excitation as claimed in claim 1, wherein the mass concentration of the plant-derived smoke water mother liquor in the second step is diluted to 1.8%.
4. The method for improving the saline-alkali resistance of the grass seeds by using the smoke water excitation as claimed in claim 1, wherein the mass concentration of the plant-derived smoke water mother liquor in the second step is diluted to 2.0%.
5. The method for improving the saline-alkali resistance of the grass seeds by using the smoke water excitation as claimed in claim 1, wherein the mass concentration of the plant-derived smoke water mother liquor in the second step is diluted to 2.5%.
6. The method for improving the saline-alkali resistance of the grass seeds by using the smoke water excitation as claimed in claim 1, wherein the mass concentration of the plant-derived smoke water mother liquor in the second step is diluted to 3%.
7. The method for improving the saline-alkali resistance of the grass seeds by using the smoke and water excitation as claimed in claim 1, wherein the vibration is performed for 20 hours at a speed of 100 revolutions per minute in the step two.
8. The method for improving the saline-alkali resistance of the grass seeds by using the smoke and water excitation as claimed in claim 1, wherein the vibration is carried out for 21 hours at a speed of 100 revolutions per minute in the step two.
9. The method for improving the saline-alkali resistance of the grass seeds by using the smoke and water excitation as claimed in claim 1, wherein the vibration is performed for 22 hours at a speed of 100 revolutions per minute in the step two.
10. The method for improving the saline-alkali resistance of the grass seeds by using the smoke and water excitation as claimed in claim 1, wherein in the second step, the vibration is carried out for 23 hours at a speed of 100 revolutions per minute.
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20140075839A1 (en) * | 2011-05-26 | 2014-03-20 | Nanjing Agricultural University | Method for improving survival rate of directly-planted seedling and transplanting seedling of haloxylon plant in desert or semi-desert environment |
| CN109042102A (en) * | 2018-08-01 | 2018-12-21 | 中储草生态农业科技有限责任公司 | A method of improving herbage seedling salt tolerant growth ability |
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- 2020-06-11 CN CN202010528810.XA patent/CN111567180A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20140075839A1 (en) * | 2011-05-26 | 2014-03-20 | Nanjing Agricultural University | Method for improving survival rate of directly-planted seedling and transplanting seedling of haloxylon plant in desert or semi-desert environment |
| CN109042102A (en) * | 2018-08-01 | 2018-12-21 | 中储草生态农业科技有限责任公司 | A method of improving herbage seedling salt tolerant growth ability |
Non-Patent Citations (1)
| Title |
|---|
| M.A. WAHEED ET AL.: "Effect of plant-derived smoke solutions onphysiological and biochemical attributes of maize (Zea mays L.) under salt stress", 《PAKISTAN JOURNAL OF BOTANY》 * |
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Application publication date: 20200825 |