CN111548971A - Preparation method of economic microalgae high-efficiency culture medium based on laver processing wastewater - Google Patents
Preparation method of economic microalgae high-efficiency culture medium based on laver processing wastewater Download PDFInfo
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Abstract
The invention discloses a preparation method of an economic microalgae high-efficiency culture medium based on laver processing wastewater and the economic microalgae high-efficiency culture medium prepared by the method, and the method mainly comprises the following steps: 1) ultrasonically crushing laver processing wastewater containing laver residues for 10-60 min, and removing impurities by adopting a centrifugation or filtration method to obtain pretreated wastewater; 2) supplementing a proper amount of nutrient substances required by the growth of microalgae in the pretreated wastewater to prepare an economic microalgae culture solution; 3) and (3) carrying out ultraviolet sterilization on the culture solution for 5-60 min to obtain the high-efficiency microalgae culture medium which can be directly used for microalgae culture. The method for preparing the efficient microalgae culture medium by using the laver processing wastewater as a raw material has the advantages of simple and convenient operation, capability of obtaining higher microalgae biomass and promoting the accumulation of high-value active substances of the microalgae, and simultaneously, capability of remarkably reducing the large-scale culture cost of the economic microalgae and effectively relieving the problem of environmental pollution caused by the discharge of the laver processing wastewater.
Description
Technical Field
The invention belongs to the technical field of comprehensive treatment and utilization of wastes in the field of agricultural product processing and microalgae culture, and particularly relates to a preparation method of an economic microalgae high-efficiency culture medium based on laver processing wastewater.
Background
Microalgae is a kind of photoautotrophic unicellular organism, and has the advantages of high photosynthetic efficiency, high growth speed, capability of rapidly accumulating a large amount of biomass, polyunsaturated fatty acids, grease, carbon compounds, proteins, amino acids, pigments and other high-value active substances, and the like. The application of the compound has already been related to the fields of food, health care products, medicines, cosmetics, aquatic feed, animal feed, wastewater treatment and the like. However, large-scale culture of microalgae requires a large amount of water resources and nutrients, so that the production cost is high, the microalgae biomass far cannot meet the current market demand, and the industrial development of microalgae is severely limited.
Laver is an economically cultivated seaweed which is very important for human dietary health, and is cultivated in many countries, especially in china, japan and korea, with a wide planting area. A large amount of wastewater with high organic matter content is generated in the laver processing process, and the direct discharge can cause serious environmental pollution problems, but the culture medium is a potential high-efficiency culture medium for some microalgae.
The microalgae can rapidly absorb and utilize a large amount of organic matters, nitrogen, phosphorus and other nutrient substances in the growth process, and has wide application prospect in the field of resource recycling of various types of wastewater. The culture medium prepared from the laver processing wastewater is used for culturing the economic microalgae, so that the environmental problem caused by wastewater discharge can be effectively relieved, the cost of laver processing wastewater treatment and microalgae large-scale culture can be effectively reduced, and water resources are saved.
Disclosure of Invention
The invention aims to provide a method for simply and efficiently recycling the nutrient substances in the laver processing wastewater to obtain microalgae biomass from the aspect of recycling waste resources, and simultaneously provides an ecological method for relieving environmental pollution caused by direct discharge of the laver processing wastewater.
In order to achieve the purpose, the technical scheme adopted by the invention is as follows:
a preparation method of an economic microalgae high-efficiency culture medium based on laver processing wastewater comprises the following steps:
(1) ultrasonically crushing laver processing wastewater containing laver residues for 10-60 min under the conditions of 100 plus 2000W, and then centrifuging or filtering at normal temperature under the conditions of 3000 plus 6000 rpm/min to remove impurities to obtain pretreated wastewater;
(2) supplementing a proper amount of macronutrients and micronutrients into the pretreated wastewater to prepare an economic microalgae culture solution;
(3) placing the prepared culture solution at intensity of 90-150 μ W/cm2Sterilizing for 5-60 min under an ultraviolet lamp to obtain the high-efficiency microalgae culture medium which can be directly used for culturing the economic microalgae.
Preferably, the method for removing impurities in the laver processing wastewater is centrifugation for 5-10min under the conditions of 3000-6000 rpm/min, or filtration by using a bolting silk.
Preferably, the culture medium prepared by the method can be used for culturing economic microalgae such as chlorella, scenedesmus obliquus, spirulina, chlamydomonas and the like to obtain biomass 0.5-6.0 times higher than that obtained by culturing the economic microalgae by a conventional culture medium, and can remarkably improve the yield of active substances such as soluble proteins, carbohydrates, grease, pigments and the like of algae cells.
Preferably, the economic microalgae are one or more of the microalgae that can be grown in a minimal medium such as BBM, BG11, TAP, Zarrouk, etc.
Preferably, the economic microalgae can accumulate higher biomass in laver processing wastewater without supplementing any nutrient, and supplementing a proper amount of nutrient can further promote the accumulation of the economic microalgae biomass.
Preferably, the macronutrients supplemented to the laver processing wastewater are one or more of sodium bicarbonate, glacial acetic acid, Tris, sodium nitrate, ammonium chloride, dipotassium hydrogen phosphate, and potassium dihydrogen phosphate.
Preferably, the concentration of the sodium bicarbonate is 0-20 g/L, the concentration of the glacial acetic acid is 0-1.2 g/L, Tris is 0-2.5 g/L, the concentration of the sodium nitrate is 0-2.5 g/L, the concentration of the ammonium chloride is 0-0.5 g/L, the concentration of the dipotassium hydrogen phosphate is 0-0.5 g/L, and the concentration of the potassium dihydrogen phosphate is 0-0.2 g/L.
Preferably, the micronutrients supplemented to the laver processing wastewater are one or more of zinc sulfate heptahydrate, copper sulfate pentahydrate and boric acid.
Preferably, the concentration of the zinc sulfate heptahydrate is 0-25 mg/L, the concentration of the copper sulfate pentahydrate is 0-2.0 mg/L, and the concentration of the boric acid is 0-12 mg/L.
The technical scheme can obtain the following beneficial effects:
(1) according to the invention, the culture medium of the economic microalgae is prepared by directly taking the laver processing wastewater as a raw material, so that the biomass and the grease yield of the economic microalgae can be obviously improved, the accumulation of active substances such as soluble protein, carbohydrate and the like of algae cells is promoted, the large-scale culture cost of the economic microalgae is effectively lowered, and the efficient utilization of the laver processing wastewater is realized.
(2) According to the invention, the laver processing wastewater is used for replacing a pure water resource to prepare the culture medium to culture the economic microalgae, and the obtained microalgae biomass can be used for developing products such as animal feed, aquatic feed, biological energy and the like, so that the economic benefit of related enterprises is effectively improved, meanwhile, the water resource is saved, the problem of environmental pollution caused by laver processing wastewater discharge is relieved, and the sustainable development of the related enterprises is promoted.
Drawings
FIG. 1 shows the growth of economic microalgae in different media compositions.
FIG. 2 economy of biomass of microalgae in different component media.
FIG. 3 specific growth rates of economic microalgae in different component media.
Detailed Description
The invention is further described below with reference to the accompanying drawings:
as shown in fig. 1 to 3, a preparation method of an economical microalgae high-efficiency culture medium based on laver processing wastewater comprises the following steps:
(1) ultrasonically crushing laver processing wastewater containing laver residues for 10-60 min under the conditions of 100 plus 2000W, and then centrifuging or filtering at normal temperature under the conditions of 3000 plus 6000 rpm/min to remove impurities to obtain pretreated wastewater;
(2) supplementing proper amounts of macronutrients such as 0-20 g/L sodium bicarbonate, 0-1.2 g/L glacial acetic acid, 0-2.5 g/L Tris (hydroxymethyl) aminomethane (Tris), 0-2.5 g/L sodium nitrate, 0-0.5 g/L ammonium chloride, 0-0.5 g/L dipotassium hydrogen phosphate, 0-0.2 g/L potassium dihydrogen phosphate and the like, and micronutrients such as 0-25 mg/L zinc sulfate heptahydrate, 0-2.0 mg/L copper sulfate pentahydrate, 0-12 mg/L boric acid and the like into the pretreated wastewater to prepare an economic microalgae culture solution;
(3) placing the prepared culture solution at intensity of 90-150 μ W/cm2Sterilizing for 5-60 min under an ultraviolet lamp to obtain the high-efficiency microalgae culture medium which can be directly used for culturing the economic microalgae.
Example 1:
ultrasonically crushing laver processing wastewater containing laver residues for 20 min under the conditions of 600W, ultrasonic wave for 3 s and interval of 5 s, and centrifuging for 5min under the conditions of normal temperature and 4000 rpm/min to remove impurities. Then 0.25 g/L sodium nitrate, 0.04 g/L dipotassium hydrogen phosphate, 0.09 g/L monopotassium phosphate, 0.79 mg/L copper sulfate pentahydrate and 5.71 mg/L boric acid are supplemented to the wastewater with impurities removed to prepare a novel efficient culture medium for microalgae which can grow in a BBM culture medium. It was measured at an intensity of 100. mu.W/cm2The chlorella F-275 is cultured after being sterilized for 20 min under an ultraviolet lamp, the biomass of the microalgae after being cultured for 7 d is 5.91 times higher than that of the microalgae cultured by BBM, the oil yield is 2.15 times higher than that of the microalgae cultured by BBM, and the soluble protein yield is 1.23 times higher than that of the microalgae cultured by BBM.
Example 2:
ultrasonically crushing laver processing wastewater containing laver residues for 20 min under the conditions of 600W, ultrasonic wave for 3 s and interval of 5 s, and centrifuging for 8min under the conditions of normal temperature and 4000 rpm/min to remove impurities. Then 0.30 g/L sodium nitrate and 0.15 g/L hydrogen phosphate are supplemented to the wastewater with impurities removedDipotassium, 0.08 mg/L copper sulfate pentahydrate and 2.86 mg/L boric acid to prepare a novel efficient culture medium for microalgae which can grow in a BG11 culture medium. It was measured at an intensity of 90. mu.W/cm2After the Scenedesmus obliquus is cultured after being sterilized for 20 min under an ultraviolet lamp, the biomass of the microalgae after being cultured for 7 d is 1.81 times higher than that cultured by BG11, the oil yield is 1.52 times higher than that cultured by BG11, and the soluble protein yield is 3.18 times higher than that cultured by BG 11.
Example 3:
ultrasonically crushing laver processing wastewater containing laver residues for 20 min under the conditions of 600W, ultrasonic wave for 3 s and interval of 5 s, and centrifuging at normal temperature and 4000 rpm/min for 10min to remove impurities. Then 1.21g/L Tris, 0.50 g/L glacial acetic acid, 0.19 g/L ammonium chloride, 0.11 g/L dipotassium hydrogen phosphate, 0.05 g/L potassium dihydrogen phosphate, 11 mg/L zinc sulfate heptahydrate, 0.79 mg/L copper sulfate pentahydrate and 5.71 mg/L boric acid are supplemented to the wastewater with impurities removed to prepare a novel efficient culture medium of the microalgae capable of growing in the TAP culture medium. It was measured at an intensity of 100. mu.W/cm2The chlamydomonas reinhardtii is cultured after the sterilization for 20 min under an ultraviolet lamp, the biomass of the microalgae after 5 days of culture is 2.37 times higher than that of TAP culture, and the grease yield is 1.57 times higher than that of TAP culture.
Example 4:
ultrasonically crushing thallus Porphyrae processing wastewater containing thallus Porphyrae residue at 600W for 3 s at 5 s interval for 20 min, and filtering with silk screen at normal temperature to remove impurities. Then 9.0 g/L sodium bicarbonate, 1.5 g/L sodium nitrate, 0.25 g/L dipotassium hydrogen phosphate, 0.08 mg/L copper sulfate pentahydrate and 2.86 mg/L boric acid are supplemented to the wastewater with impurities removed to prepare a novel efficient culture medium for microalgae which can grow in the Zarrouk culture medium. It was measured at an intensity of 90. mu.W/cm2The spirulina platensis is cultured after the sterilization for 20 min under an ultraviolet lamp, the biomass of the microalgae after 7 d culture is 1.80 times higher than that of the microalgae cultured by Zarrouk, the grease yield is 1.26 times higher than that of the microalgae cultured by Zarrouk, and the carbohydrate yield is 0.83 times higher than that of the microalgae cultured by Zarrouk.
In FIG. 1, a comparison graph of conventional culture medium culture and the culture mediums 1 and 2 prepared by the method of the present invention for culturing economic microalgae is shown, and through the growth of the economic microalgae chlorella F-275 in different culture mediums, it can be found that the microalgae cultured by the conventional culture medium is basically unchanged along with the increase of time, and after the method of example 1 is adopted, the microalgae cultured by the two culture mediums 1 and 2 grow better and better along with the increase of time.
Similarly, in example 1, the economic microalgae chlorella F-275 and FIG. 2, the biomass accumulation of the economic microalgae chlorella F-275 in the culture media with different components is shown by a graph, the biomass of the microalgae cultured by the conventional culture medium is very slowly increased along with the time, while the biomass of the culture media 1 and 2 prepared by the method of the invention is gradually increased from 200 mg/L to 500 mg/L along with the time, and the biomass of the economic microalgae can be obviously improved after the technology of the invention is adopted.
FIG. 3 is an evaluation of the specific growth rate of economic microalgae of culture media 1 and 2, and also the specific growth rate of economic microalgae chlorella F-275 cultured by the conventional culture media is basically unchanged and maintained at about 0.1 along with the prolonging of the culture time, while the specific growth rate of 1.2-1.6 can be reached at the beginning by the microalgae cultured by the culture media 1 and 2 prepared by the method of the invention, and the specific growth rate is gradually reduced along with the increasing of the culture time due to the growth characteristics of the microalgae, but the final specific growth rate can also reach about 0.4, which is obviously higher than that of the conventional culture media.
The results are comprehensively analyzed, the laver processing wastewater is directly used as a water resource to replace a novel high-efficiency microalgae culture medium prepared by pure water, the biomass and the grease yield of economic microalgae can be obviously improved, the accumulation of active substances such as soluble protein and carbohydrate of algae cells is promoted, the method can effectively save the water resource, the problem of environmental pollution caused by the discharge of the laver processing wastewater is relieved, the culture cost of the microalgae can be reduced, and the method has a good promotion effect on the industrial development of the microalgae.
The above description is only an example of the preferred embodiment of the present invention, and is not intended to limit the technical scope of the present invention in any way, and all equivalent modifications made according to the technical solutions of the present invention should fall within the protection scope of the present invention.
Claims (9)
1. A preparation method of an economic microalgae high-efficiency culture medium based on laver processing wastewater is characterized by comprising the following steps: the method comprises the following steps:
(1) ultrasonically crushing laver processing wastewater containing laver residues for 10-60 min under the conditions of 100 plus 2000W, and then centrifuging or filtering to remove impurities under the conditions of normal temperature and 3000 plus 6000 rpm/min to obtain pretreated wastewater;
(2) supplementing a proper amount of macronutrients and micronutrients into the pretreated wastewater to prepare an economic microalgae culture solution;
(3) placing the prepared culture solution at intensity of 90-150 μ W/cm2Sterilizing for 5-60 min under an ultraviolet lamp to obtain the high-efficiency microalgae culture medium which can be directly used for culturing the economic microalgae.
2. The method for preparing the economic microalgae efficient culture medium based on laver processing wastewater as claimed in claim 1, wherein the economic microalgae efficient culture medium comprises the following components: the method for removing impurities in the laver processing wastewater is to centrifuge for 5-10min under the conditions of 3000-6000 rpm/min or filter by using bolting silk.
3. The method for preparing the economic microalgae efficient culture medium based on laver processing wastewater as claimed in claim 1, wherein the economic microalgae efficient culture medium comprises the following components: the culture medium prepared by the method can be used for culturing economic microalgae such as chlorella, scenedesmus obliquus, spirulina, chlamydomonas and the like to obtain biomass 0.5-6.0 times higher than that obtained by culturing the economic microalgae by the conventional culture medium, and can obviously improve the yield of active substances such as soluble proteins, carbohydrates, grease, pigments and the like of algae cells.
4. The method for preparing the economic microalgae efficient culture medium based on laver processing wastewater as claimed in claim 1, wherein the economic microalgae efficient culture medium comprises the following components: the economic microalgae is one or more of microalgae which can grow in a minimal medium such as BBM, BG11, TAP, Zarrouk and the like.
5. The method for preparing the economic microalgae efficient culture medium based on laver processing wastewater as claimed in claim 1, wherein the economic microalgae efficient culture medium comprises the following components: the economic microalgae can accumulate higher biomass in the laver processing wastewater without supplementing any nutrient substances, and the supplement of a proper amount of nutrient substances can further promote the accumulation of the economic microalgae biomass.
6. The method for preparing the economic microalgae efficient culture medium based on laver processing wastewater as claimed in claim 1, wherein the economic microalgae efficient culture medium comprises the following components: the abundant nutrition supplemented to the laver processing wastewater is one or more of sodium bicarbonate, glacial acetic acid, Tris, sodium nitrate, ammonium chloride, dipotassium hydrogen phosphate and potassium dihydrogen phosphate.
7. The method for preparing the economic microalgae efficient culture medium based on the laver processing wastewater as claimed in claim 1 or 6, wherein the economic microalgae efficient culture medium comprises the following components: the concentration of the sodium bicarbonate is 0-20 g/L, the concentration of the glacial acetic acid is 0-1.2 g/L, Tris is 0-2.5 g/L, the concentration of the sodium nitrate is 0-2.5 g/L, the concentration of the ammonium chloride is 0-0.5 g/L, the concentration of the dipotassium phosphate is 0-0.5 g/L, and the concentration of the potassium dihydrogen phosphate is 0-0.2 g/L.
8. The method for preparing the economic microalgae efficient culture medium based on laver processing wastewater as claimed in claim 1, wherein the economic microalgae efficient culture medium comprises the following components: the micronutrient supplemented to the laver processing wastewater is one or more of zinc sulfate heptahydrate, copper sulfate pentahydrate and boric acid.
9. The method for preparing the economic microalgae efficient culture medium based on the laver processing wastewater as claimed in claim 1 or 8, wherein the economic microalgae efficient culture medium comprises: the concentration of the zinc sulfate heptahydrate is 0-25 mg/L, the concentration of the copper sulfate pentahydrate is 0-2.0 mg/L, and the concentration of the boric acid is 0-12 mg/L.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN113528421A (en) * | 2021-08-31 | 2021-10-22 | 四川大学 | Zinc ion-containing microalgae culture medium and microalgae culture method |
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