CN111544519B - Preparation method of honey-processed rhizoma polygonati - Google Patents

Abstract

The invention discloses a preparation method of honey-processed rhizoma polygonati, which comprises the following steps: slicing rhizoma polygonati, adding refined honey, moistening for 16-20 h, stewing for 12-16 h, stewing for 10-14 h, and drying to obtain the sealwort. The preparation method of the honey-processed polygonatum sibiricum has simple process, reduces the production cost and simultaneously maintains the good drug effect of the polygonatum sibiricum. The honey-processed rhizoma polygonati decoction pieces obtained by the preparation method have high content of rhizoma polygonati polysaccharide, total saponin and diosgenin which are main active ingredients, and have obvious cough relieving effect and clinical application value as proved by animal experiments.

Description

Preparation method of honey-processed rhizoma polygonati

Technical Field

The invention particularly relates to a preparation method of honey-processed rhizoma polygonati.

Background

Rhizoma Polygonati has effects of invigorating qi, nourishing yin, invigorating spleen, moistening lung, and invigorating kidney. It is commonly indicated for qi deficiency of spleen and stomach, lassitude, stomach yin deficiency, dry mouth, poor appetite, lung deficiency and dry cough. In ancient or modern times, rhizoma Polygonati is often used as a processed product. Honey has the effects of tonifying middle-jiao and moistening dryness, moistening lung and relieving cough, and is sweet and slow, and capable of benefiting spleen and correcting taste, is often used as an important auxiliary material for processing traditional Chinese medicines, and the effect of honey is very well regarded by traditional medicine. The refined honey is used for processing the sealwort, so the sealwort is sweet and tasty to eat, and is commonly used for lung deficiency and dry cough, spleen and stomach weakness and essence deficiency due to kidney deficiency. For example, fructus Lycii pill can be used for treating kidney essence deficiency, dizziness, and soft feet; when encountering the immortal life-prolonging pill, the sealwort honey is steamed and dried for 9 times, and the effects of nourishing yin and supplementing blood, and tonifying liver and kidney yin deficiency can be achieved.

At present, the processing method of polygonatum sibiricum is recorded in chinese pharmacopoeia (2015 edition): rhizoma polygonati: removing impurities, cleaning, moistening, slicing, and drying; ② steaming (or stewing) with wine: mixing rhizoma Polygonati with wine, placing in a suitable container, heating and steaming or taking out when the required degree is reached, and drying. The processing method for recovering rhizoma Polygonati from the Chinese medicine processing universities includes: firstly, cooking yellow essence: cleaning raw medicinal materials, steaming for 4-6 h (above atmospheric time), stewing overnight, taking out, slicing into thick slices or sections, uniformly stirring steaming liquid, repeatedly steaming and drying for 2-3 times, and steaming to obtain black and moist food with sweet taste and no numb taste. Air drying or oven drying; ② wine sealwort: cleaning raw materials, sun drying, mixing with yellow wine, placing in a pot, sealing, heating over water until the wine is absorbed completely. Or placing in a container, steaming to black, and moistening. Taking out, drying to slightly dry, slicing into thick pieces, and drying. Yellow wine 20kg or Chinese liquor 10kg is used for every 100kg of rhizoma Polygonati. According to the traditional preparation method, the rhizoma polygonati can meet the processing requirements of moist black inside and outside and sweet black color only by continuously steaming for more than 12 hours. However, the honey-processed polygonatum rhizome has not been prepared by a standardized preparation process, and patent CN106234734A discloses a preparation method of nutritional honey-processed polygonatum rhizome, and the honey-processed polygonatum rhizome prepared by the method has good taste but undefined curative effect.

Therefore, the honey-processed polygonatum sibiricum decoction pieces with definite curative effects are researched and developed, and have great social significance and economic value.

Disclosure of Invention

In order to solve the problems, the invention provides a preparation method of honey-processed rhizoma polygonati, which comprises the following steps:

slicing rhizoma polygonati, adding refined honey, moistening for 16-20 h, stewing for 12-16 h, stewing for 10-14 h, and drying to obtain the polygonatum.

Further, the mass ratio of the rhizoma polygonati to the refined honey is 15-25: 100.

Furthermore, the mass ratio of the rhizoma polygonati to the refined honey is 20-25: 100, preferably 20: 100.

further, the rhizoma polygonati is cleaned, impurity-removed and cut into pieces with the thickness of 3-4 mm.

Further, the moistening is closed moistening, and the closed moistening is performed for 16-18 h, preferably 16 h.

Further, the stewing is carried out in a closed container, and heating is carried out in a water-proof manner for 14-16 h, preferably 16 h.

Further, the stewing is carried out by turning off the fire for 12 hours.

Further, drying to a moisture content of less than 15%.

The invention also provides honey-processed rhizoma polygonati decoction pieces, which are characterized in that: the decoction pieces are prepared according to the method.

The invention finally provides application of honey-processed rhizoma polygonati decoction pieces in preparing a medicine with a cough relieving effect.

The preparation method of the honey-processed polygonatum sibiricum disclosed by the invention is simple in process, reduces the production cost and simultaneously maintains the good drug effect of the polygonatum sibiricum. The honey-processed rhizoma polygonati decoction pieces obtained by the preparation method have high content of rhizoma polygonati polysaccharide, total saponin and diosgenin which are main active ingredients, and have obvious cough relieving effect and clinical application value as proved by animal experiments.

Obviously, many modifications, substitutions, and variations are possible in light of the above teachings of the invention, without departing from the basic technical spirit of the invention, as defined by the following claims.

The present invention will be described in further detail with reference to the following examples. This should not be understood as limiting the scope of the above-described subject matter of the present invention to the following examples. All the technologies realized based on the above contents of the present invention belong to the scope of the present invention.

Detailed Description

Example 1 processing of Polygonatum sibiricum Red

Cleaning rhizoma Polygonati, removing impurities, slicing into 3-4mm, adding 20% refined honey, sealing, moistening for 16 hr until liquid adjuvants are completely absorbed, placing in a sealed stewing container, stewing with flowing steam at 100 deg.C for 16 hr, stewing with closed fire for 12 hr, taking out, and drying until water content is less than 15%.

Example 2 processing Polygonatum sibiricum Red

Cleaning rhizoma Polygonati, removing impurities, slicing into 3-4mm, adding 20% refined honey, sealing, moistening for 18 hr until liquid adjuvants are completely absorbed, placing in a sealed stewing container, stewing with flowing steam at 100 deg.C for 16 hr, stewing with closed fire for 12 hr, taking out, and drying until water content is less than 15%.

Example 3 processing rhizoma Polygonati

Cleaning rhizoma Polygonati, removing impurities, slicing into 3-4mm, adding 20% refined honey, sealing, moistening for 16 hr until liquid adjuvants are completely absorbed, placing in a sealed stewing container, stewing with flowing steam at 100 deg.C for 14 hr, stewing with closed fire for 12 hr, taking out, and drying until water content is less than 15%.

The advantageous effects of the present invention will be described below by way of test examples.

Test example 1 preparation of honey-processed polygonatum sibiricum

1. Material

PS-80 ultrasonic cleaner (Shenzhen Jiekang cleaning electric appliance Co., Ltd.); JP-350A-8 model high-speed multi-function pulverizer (Shanghai Bingdu electric Co., Ltd.); UV-6100 type ultraviolet-visible spectrophotometer (Shanghai Mei spectral instruments, Inc.); the Agilent1200 high performance liquid chromatograph comprises G1322A (quaternary pump), G1329A/B (automatic temperature control autosampler), G1316A (column oven), G1315BD (diode array detector);

Rhizoma Polygonati and Mel are purchased from flos Nelumbinis pool medicinal materials market;

anhydrous glucose (Chinese medicine biological product institute, 110833-.

2. Research on process for stewing polygonatum sibiricum with honey

2.1 selection of factor levels

According to long-term practice, the effect of the honey processing technology is related to auxiliary materials, moistening time and heating time, so that during factor investigation, the honey refining amount, the honey moistening time, the stewing time and the like are selected as screening factors. The stewing time is not taken as a screening factor, and the stewing time is determined to be 12 hours in consideration of the feasibility of mass production and the sufficient cooling of the medicine. Therefore, the material-liquid ratio (A), the honey moistening time (B) and the honey stewing time (C) are finally selected as investigation factors to carry out the test, the multi-index evaluation is carried out by adopting an analytic hierarchy process, the comprehensive scores of all indexes are used as the investigation indexes, and the polygonatum honey stewing process is further preferably selected, and is shown in table 1.

TABLE 1 test factor level table

2.2 test Schedule

With the selection of the factor levels of table 1, a 10-run schedule was carried out, see table 2, with 200g of feed per run.

TABLE 2 Honey stewed rhizoma Polygonati test arrangement table

2.3 determination of evaluation index

The content of the internal components of the decoction pieces and the comprehensive weighted score for protecting the mouse model with the cough caused by ammonia water are selected as evaluation indexes.

2.3.1 content of intrinsic ingredients

The content limit control of rhizoma polygonati tablets is regulated in the 2015 edition of Chinese pharmacopoeia, and the content of the rhizoma polygonati tablets is not less than 7.0 percent in terms of polysaccharide; in addition, polysaccharide and saponin components are the main sources of the sweet taste of the rhizoma polygonati and also the main material basis of the 'nature and taste' of the rhizoma polygonati; the rhizoma Polygonati polysaccharide has antitumor, blood sugar lowering, immunity regulating, antibacterial and anti-inflammatory effects, and total saponin can enhance immunity of chronic stress depression model rat. The steroid saponin component in the rhizoma Polygonati saponin is important component. The steroid saponin components include diosgenin, sarsasapogenin, etc., and mainly comprise diosgenin. Diosgenin has obvious pharmacological action, has antitumor, cardiovascular system resisting and estrogen-like effects, and is a precursor for synthesizing steroid hormone medicines. Therefore, the polysaccharide, the total saponin and the diosgenin are considered as main drug effect substance bases, and are selected as quality standards for research. The overall weighted score was 30 points, 10 points for each component.

2.3.2 protective action on mouse model with cough caused by ammonia water

Considering that the internal components of the traditional Chinese medicine possibly have content reduction or transformation phenomena in the processing and heating process of the traditional Chinese medicine, and the processing purpose of the product is mainly used for lung dryness cough, more consideration is given to the evaluation of pharmacodynamic indexes in the overall weighted score, and the full score is 70 by taking the protection effect of a mouse model with the cough caused by ammonia water as the evaluation index.

3 methods and results

3.1 measurement of content of intrinsic component

3.1.1 determination of polysaccharides

(1) Preparation of control solutions

Taking 33.77mg of anhydrous glucose reference substance dried to constant weight at 105 ℃, precisely weighing, placing in a 100ml measuring flask, adding water to dissolve, diluting to scale, and shaking up to obtain the final product (0.3377 mg of anhydrous glucose is contained in each 1 ml).

(2) Preparation of the Standard Curve

Precisely measuring reference substance solutions 0.1ml, 0.2ml, 0.3ml, 0.4ml, 0.5ml and 0.6ml, respectively placing in 10ml test tubes with scales, adding water to 2.0ml, shaking, slowly dripping 0.2% anthrone-sulfuric acid solution in ice water bath to scales, mixing, cooling, placing in water bath, keeping the temperature for 10 min, taking out, immediately placing in ice water bath, cooling for 10 min, taking out, and taking corresponding reagents as blank. Measuring absorbance (A) at 582nm by ultraviolet-visible spectrophotometry (general rule 0401), taking A as ordinate and mass concentration (C) as abscissa, obtaining regression equation A of 57.807C +0.1459(R2 of 0.9961), which indicates that the content of glucose is 0.00266-0.0225 mg/mL ^－1Is in good linear relationship with A.

(3) Assay method

Taking about 0.25g of fine powder of the product dried to constant weight at 60 ℃, precisely weighing, placing the fine powder in a round-bottom flask, adding 150ml of 80% ethanol, placing the round-bottom flask in a water bath for heating and refluxing for 1 hour, filtering while the fine powder is hot, washing the residue with 80% hot ethanol for 3 times, l0ml each time, placing the residue and filter paper in the flask, adding 150ml of water, placing the round-bottom flask in a boiling water bath for heating and refluxing for 1 hour, filtering while the residue and the flask are hot, l0ml each time, combining filtrate and washing liquor, cooling, transferring the mixture into a 250ml measuring flask, adding water to a scale, shaking uniformly, precisely measuring 1ml, placing the test tube in a l0ml stopper drying test tube, measuring absorbance according to a method under the preparation item of a standard curve, starting from 'adding water to 2.0 ml', reading the weight (mg) of anhydrous glucose in a solution of the product from the standard curve, and calculating to obtain the product.

(4) Determination of precision, repeatability, stability and sample recovery

The control solution with the same concentration is taken, repeated measurement is carried out for 6 times, the precision is examined, the absorbance RSD is 0.94%, and the precision of the instrument is good. And (3) preparing a sample solution from 6 parts of the same sample according to the steps listed in the item (2), carrying out sample injection measurement according to the ultraviolet spectrophotometer condition under the item (2), and investigating repeatability, wherein the RSD content of the polysaccharide in the sample is 1.36%, which indicates that the method has good repeatability. And (3) taking the same sample solution, sequentially injecting samples for 0, 4, 8, 12, 16, 20 and 24 hours under the condition of the ultraviolet spectrophotometer under the item (2), and indicating that the absorbance RSD of the polysaccharide is 0.99 percent, which indicates that the sample is relatively stable within 24 hours. Taking 0.125g of honey-stewed rhizoma Polygonati decoction piece powder with known content, precisely weighing, adding a certain amount of reference substance, preparing test sample solution according to the method in item (3), introducing sample for measurement, and inspecting sample recovery rate. The results are shown in Table 3.

TABLE 3 polysaccharide sample recovery results

3.1.2 determination of Total saponins

(1) Preparation of control solutions

Accurately weighing 10.71mg of ginsenoside Rb1 reference substance, placing in a 10mL measuring flask, adding methanol to dissolve, diluting to scale, and shaking.

(2) Preparation of the Standard Curve

Precisely measuring 0.06 mL, 0.12 mL, 0.18 mL, 0.24 mL, 0.30 mL, 0.36 mL and 0.42mL of ginsenoside Rb1 reference substance solution, respectively placing in a test tube with a plug scale, volatilizing the solution, adding 0.2mL of 5% vanillin-glacial acetic acid solution, adding 0.8mL of perchloric acid during ice bath, shaking up, heating in a 60 ℃ water bath for 15min, carrying out ice bath for 2min, adding 5mL of glacial acetic acid, shaking up, and standing for 5 min. Measuring absorbance at 543nm by ultraviolet-spectrophotometry using corresponding reagent as blank(A) Taking A as an ordinate and C as an abscissa, the regression equation A is 20.178C +0.061(r is 0.9994), and the linear range is 0.01-0.07 g.L^－1。

(3) Preparation of test solution

Weighing 1.00g of 9 batches of rhizoma polygonati powder dried to constant weight, adding 15mL of 80% ethanol, carrying out ultrasonic extraction at 60 ℃ for 50min, extracting for 2 times, filtering, combining the filtrates, putting into a 50mL measuring flask, adding 80% ethanol, and fixing the volume to scale to obtain the rhizoma polygonati powder. Precisely sucking 0.04mL of extracting solution, placing in a 10mL test tube with a plug, volatilizing ethanol, adding 5% vanillin-glacial acetic acid solution (fresh) 0.2mL, adding perchloric acid solution 0.8mL in ice bath, shaking uniformly, heating in a water bath at 60 ℃ for 15min, carrying out ice bath for 2min, adding glacial acetic acid 5mL, shaking uniformly, and standing for 5 min.

(4) Determination of precision, repeatability, stability and sample recovery

Taking a control solution with the same concentration, repeatedly injecting 10 mu L of the control solution for 6 times, and inspecting the precision, wherein the peak area RSD is 1.27%, which indicates that the precision of the instrument is good. Taking 6 parts of the same sample to prepare a sample solution according to the steps listed in the step (3), carrying out sample injection measurement according to the ultraviolet spectrophotometer condition under the item (2), and investigating repeatability, wherein the content RSD of the total saponin in the sample is measured to be 1.25%, which indicates that the method has good repeatability. And (3) taking the same sample solution, sequentially injecting samples for 0, 4, 8, 12, 16, 20 and 24 hours under the ultraviolet spectrophotometer condition of the item (2), and indicating that the sample is relatively stable within 24 hours as the result that the total saponin peak area RSD is 0.61%. Taking 0.5g of honey-stewed rhizoma Polygonati decoction piece powder with known content, precisely weighing, adding a certain amount of reference substance, preparing test sample solution according to the method in item (3), introducing sample for determination, and inspecting sample recovery rate. The results are shown in Table 4.

TABLE 4 Total saponins sample recovery results

3.1.3 determination of diosgenin content

(1) Preparation of control solutions

Taking appropriate amount of diosgenin reference, precisely weighing, and dissolving with methanol to obtain 0.532mg/mL reference solution.

(2) Preparation of test solution

1g of dried and crushed rhizoma polygonati powder is taken respectively, precisely weighed, placed in a flat-bottomed flask, precisely added with 50mL of absolute ethyl alcohol, shaken up, weighed, heated and refluxed for 4 hours, cooled, weighed again, added with ethanol to complement the weight loss, shaken up and filtered. Precisely taking 20mL of subsequent filtrate, evaporating to dryness, adding 80mL of 3mol/L hydrochloric acid solution into residues for dissolving, refluxing and hydrolyzing for 4h by boiling water, cooling, transferring to a separating funnel, adding 40mL (20mL, 10mL and 10mL) of petroleum ether (60-90 ℃) for 3 times, combining the petroleum ether (60-90 ℃) solutions, recovering the petroleum ether (60-90 ℃) under reduced pressure till the petroleum ether is dry, adding methanol into the residues for dissolving, transferring to a 2mL volumetric flask, adding methanol for diluting to a scale, and shaking up. Filtering with 0.45 μm microporous membrane before sample injection to obtain sample solution.

(3) Chromatographic conditions

Column, DL Column, Column Cig (250mmx4.6mm,5 μm); mobile phase acetonitrile, water (90: 10); the detection wavelength is 203 nm; the flow rate is 1 mL/min; the column temperature is 30 ℃; the sample injection amount is 20 mu L.

(4) Drawing of standard curve

Precisely measuring a proper amount of the reference solution and diluting the reference solution into 6 solutions with different mass concentrations according to a multiple relation. And (4) respectively injecting 10 mu L of sample under the chromatographic conditions of the item (3) for analysis, and drawing a curve by taking the concentration as an abscissa and taking a peak area as an ordinate. The standard curve is that y is 5771.2x-16.22, R ²Is 1, and the linear range is 0.36-743.2 mu g/mL.

(5) Assay method

Taking 10 μ L of standard sample and 20 μ L of sample, and injecting into high performance liquid for detection.

(6) Determination of precision, repeatability, stability and sample recovery

Taking a reference substance solution with the same concentration, repeatedly injecting sample for 6 times, wherein each time is 10 mu L, and inspecting the precision, wherein the peak area RSD is 0.23 percent, which indicates that the precision of the instrument is good. And (3) preparing a test solution from 6 parts of the same test sample according to the steps listed in the step (2), carrying out sample injection measurement according to the chromatographic conditions in the step (3), and investigating repeatability, wherein the content RSD of the diosgenin in the sample is 1.38%, which indicates that the method has good repeatability. And (3) sampling the same sample solution for 0, 4, 8, 12, 16, 20 and 24 hours in sequence according to the chromatographic conditions, wherein the area RSD of the diosgenin is 0.92%, which indicates that the sample is relatively stable within 24 hours. Taking 0.3g of honey-stewed rhizoma polygonati decoction piece powder with known content, precisely weighing, adding a certain amount of reference substances, preparing a sample solution according to the method in the item (2), carrying out sample injection measurement, and inspecting the sample injection recovery rate. The results are shown in Table 5.

TABLE 5 diosgenin Loading recovery results

The content control indexes of the internal components of the rhizoma polygonati mainly comprising polysaccharide, total saponin and diosgenin are reflected, the overall weighted score is 30 points, and each component is 10 points. The sample with the highest content in 10 test samples is full score, and the other samples are relative scores. The results are shown in Table 6.

TABLE 6 evaluation of inherent component content of experimental sample of stewed rhizoma Polygonati with honey

Note: (1) the polysaccharide content score was determined as 10 points with the highest content of test 10 samples;

(2) the total saponin content score is 10 points according to the highest content of a test 8 sample;

(3) the diosgenin score was determined to be 10 points with the highest content of test 6 samples.

3.2 protection against Ammonia-induced cough in mouse model

(1) Preparation of aqueous extracts

The polygonatum is 9-15 g, the maximum is 15g, and the weight of an adult is calculated according to 70kg, wherein the administration amount of the polygonatum is regulated to be 9-15 g for 1 day of the adult in 2015 edition. The equivalent dose of the mouse is 9.1 times of that of an adult, the high dose group is 18.2 times of that of the adult, and the rhizoma polygonati administration in the experiment is uniformly given according to the high dose. That is, 0.215g crude drug/kg × 18.2 ═ 3.9g crude drug/kg, and the volume of drug administered to mice was calculated as 0.4ml/20g, and a drug solution of 0.2g crude drug/ml was required. Taking 9 batches of raw rhizoma polygonati and honey-stewed rhizoma polygonati, adding water to extract for 3 times, each time extracting for 100ml, filtering, combining 3 times of filtrates, and concentrating under reduced pressure to 100 ml.

(2) Molding method

After the animals are normally raised for 3 days, the animals are subjected to 1-time prophylactic gavage administration for 1 day with 0.2mL each time for 7 days continuously, 1 hour after the last administration (7 th day), the mice are placed in a 500mL beaker, cotton balls with the weight of about 200mg are placed in the beaker, 200 mu L of ammonia water (with the concentration of 25-28%) is precisely transferred onto the cotton balls, the mice are reversely buckled in the beaker, the time of the first cough of the mice within 2min is observed, the incubation period of the cough (the contraction of abdominal muscles of the mice, the mouth of the mice is enlarged, and sometimes the cough sound is audible) of the mice is recorded, the cotton balls are immediately taken out after 2min, the observation is continued, and the cough frequency of the mice within 2min is recorded.

(3) Cough prescreening

Taking 120 healthy SPF-grade KM mice, putting the mice into a 500mL beaker, putting a cotton ball with the weight of about 250mg into the beaker, precisely transferring 200 mu L (the concentration is 25-28%) of ammonia water onto the cotton ball, simultaneously, reversely buckling the mice in the beaker, and observing the time for the mice to cough for the first time within 2min and the cough sensitivity.

(4) 106 primarily screened animals are weighed in groups and randomly divided into 13 groups according to body weight, and 8-9 animals are used in each group.

Model comparison group

② stewing rhizoma polygonati No. 1 sample group with honey;

③ stewing rhizoma polygonati with honey in a sample group No. 2;

fourthly, stewing the polygonatum sibiricum sample group No. 3 with honey;

stewing the sample group No. 4 of the sealwort with honey;

sixthly, stewing the sample group No. 5 with honey;

seventhly, stewing the rhizoma polygonati 6 sample group with honey;

eighthly, stewing the sealwort with honey in a No. 7 sample group;

ninthly, stewing the rhizoma polygonati 8 sample group with honey;

sample group No. 9 stewed with Oncorzonum sonchifolia;

stewing rhizoma Polygonati No. 10 with honey;

raw water of rhizoma polygonati;

the preparation method is selected from the dextromethorphan hydrobromide dispersible tablets (yang medicine) group.

(5) Dosing regimens and assays

After the animals are conventionally bred for 3 days, purified water is given to the first group, corresponding rhizoma polygonati liquid medicine (0.2g crude drug/ml) is given to the second to (12) groups, dextromethorphan hydrobromide dispersible tablets (0.6mg/ml) are given to the first group, the administration volume of each group is 0.4ml/20g body weight, and the animals are continuously perfused for 7 days.

Animals were fasted for 16 hours from day 6 and then were subjected to ammonia induced cough test 1 hour after the last (day 7) administration.

(6) Statistical method

For data

Showing that SPSS20.0 software statistics is used for carrying out variance analysis and multiple comparison statistical analysis of completely random design data, and adopting Dunnet test to meet the normality and the homogeneity of variance, otherwise adopting Tamhane's T2 or post-data transformation test.

(7) Test results

The influence of honey-stewed polygonatum sibiricum test samples on the cough incubation period and the cough frequency of a mouse model with the cough caused by ammonia water is shown in table 7.

TABLE 7 influence of honey-stewed rhizoma Polygonati test sample on mouse model with acute cough caused by ammonia water

As can be seen from Table 7, the cough latency and the cough frequency of the sample group of 1-10 processed with honey stewed sealwort are reduced compared with the model control group. Compared with a model control group, the No. 2-10 sample group has remarkably reduced cough latency and cough frequency, and P is less than 0.05.

Compared with a model control group, the dextromethorphan hydrobromide dispersible tablet group has remarkably reduced cough latency and cough frequency, and P is less than 0.01.

The index of the cough relieving effect of the sealwort is reflected, and the overall weighted score is 70 points. Of the 10 test samples, the sample with the best reduction rate of cough suppressing effect was full score, and the other samples were relative scores. The results are shown in Table 8.

TABLE 8 score of cough-relieving action of test sample of stewed sealwort with honey

Note: (1) the highest score of the yang medicine group sample with the longest latency period is determined as 30 points.

(2) The highest score was assigned to 40 for the test 5 sample with the least number of coughs.

3.3 test Total score

The contents of the internal components and the cough relieving effect are scored and integrated to obtain the total score of the test, and the result is shown in a table 9.

TABLE 9 test results of rhizoma Polygonati stewed with honey

And (4) analyzing results: the test results in table 9 show that the factor material-liquid ratio (a) and the honey stewing time (C) have significant influence on the overall rating of the polygonatum sibiricum, the honey moistening time (B) has no significant influence on the overall rating of the polygonatum sibiricum, and the factor D (blank column) is taken as an error. The result shows that when the ratio of the polygonatum sibiricum to the refined honey is 20-25: 100, the honey moistening time is 16-18 h, the honey stewing time is 14-16 h, the prepared honey-stewed polygonatum rhizome has higher effective components and better cough relieving effect, the total score is higher than 94%, and when the technological composition A of the honey-stewed polygonatum rhizome is₂B₁C₃The method comprises the following steps:

namely, the clean rhizoma polygonati is taken, sliced, added with 20 percent of refined honey, sealed and moistened for 16 hours until liquid auxiliary materials are basically absorbed completely, placed in a stewing container, stewed for 16 hours by using circulating steam at 100 ℃, stewed for 12 hours by turning off fire, taken out and dried, and the obtained honey-processed rhizoma polygonati has the best overall effect and the score is up to more than 98 percent.

In conclusion, the invention has simple process, reduces the production cost and simultaneously keeps the good drug effect of the rhizoma polygonati. The honey-processed rhizoma polygonati decoction pieces obtained by the preparation method have high content of rhizoma polygonati polysaccharide, total saponin and diosgenin which are main active ingredients, and have obvious cough relieving effect and clinical application value as proved by animal experiments.

Claims (4)

1. A preparation method of honey-processed rhizoma polygonati is characterized by comprising the following steps: the method comprises the following steps:

slicing rhizoma Polygonati into 3-4mm slices, adding refined honey, sealing, moistening for 16 hr, placing in a sealed container, heating over water for 16 hr, and stewing for 12 hr, and drying until water content is less than 15%;

the mass ratio of the rhizoma polygonati to the refined honey is 20: 100.

2. The method of claim 1, wherein: the sealwort is cleaned to remove impurities.

3. The honey-processed rhizoma polygonati decoction pieces are characterized by comprising the following components in parts by weight: the decoction pieces prepared by the method of any one of claims 1 to 2.

4. The use of the honey-processed rhizoma polygonati decoction pieces of claim 3 in preparing a medicine with a cough relieving effect.