CN111534430A

CN111534430A - Ribonucleic acid detection panel and ribonucleic acid detection device

Info

Publication number: CN111534430A
Application number: CN202010350040.4A
Authority: CN
Inventors: 江立达; 邱伯韦; 范翔瑜; 曾士修; 萧建仁; 张丽樱
Original assignee: Keycore Technology Corp
Current assignee: Gangxiu Technology Shanghai Co ltd
Priority date: 2020-04-28
Filing date: 2020-04-28
Publication date: 2020-08-14
Anticipated expiration: 2040-04-28
Also published as: CN111534430B

Abstract

The invention provides a ribonucleic acid detection panel and a ribonucleic acid detection device, wherein the ribonucleic acid detection device comprises a control unit and a ribonucleic acid detection panel, the ribonucleic acid detection panel comprises a substrate, a plurality of induction electrode layers electrically connected with the control unit, at least one primer layer and a plurality of routing layers, the plurality of induction electrode layers are arranged on the first surface of the substrate, the at least one primer layer is arranged on the plurality of induction electrode layers and is insulated from each other, and the plurality of electrode routing layers are electrically connected with the plurality of induction electrodes and the control unit.

Description

Ribonucleic acid detection panel and ribonucleic acid detection device

Technical Field

The present invention relates to a panel and a device for detecting ribonucleic acid, and more particularly, to a panel and a device for detecting ribonucleic acid, which can shorten the detection time and reduce the cost.

Background

Since the disease condition of the user (such as kawasaki disease, colorectal cancer, hand-foot-mouth disease, novel coronavirus (COVID-19) or other RNA-bearing viruses) must be detected by the measurement platform of the hospital for marking (such as a Polymerase Chain Reaction (PCR) analyzer), then blood of the user is collected, miRNA virus is extracted by an RNA extraction instrument, miRNA is added in sequence, miRNA labeling reagent is added, and the miRNA labeling reagent is dropped onto a miRNA wafer for dyeing and scanning, so as to determine whether the user has a diagnosis according to the fluorescence brightness change and comparison data, professional instruments (such as the measurement platform and the RNA extractor) are required in the conventional method for detecting RNA (or miRNA) viruses, so that only specific professional medical staff can operate the professional instruments, thereby causing the problems of high detection difficulty and high cost. In addition, the conventional procedure for detecting virus by fluorescence labeling is complicated and complex, so that the detection time is long due to the fact that the detection time is at least 2 hours or more or two days or more.

Disclosure of Invention

An object of the present invention is to provide a ribonucleic acid detection panel that can achieve a reduction in detection time and cost.

Another object of the present invention is to provide a ribonucleic acid detection panel which is simple in detection operation and convenient in detection.

To achieve the above object, the present invention provides a ribonucleic acid detecting panel, which includes a substrate, a plurality of sensing electrode layers, at least one primer layer and a plurality of electrode routing layers, wherein the substrate has a first surface and a second surface opposite to the first surface, the plurality of sensing electrode layers are disposed on the first surface, the at least one primer layer is disposed on the plurality of sensing electrode layers and insulated from each other, the at least one primer layer is for reacting with a corresponding detecting body having ribonucleic acid, the plurality of electrode routing layers are disposed on the first surface, and the plurality of electrode routing layers are electrically connected to the plurality of sensing electrodes.

The first surface of the substrate is provided with an induction area and a peripheral area surrounding the induction area, the induction electrode layers are arranged on the first surface of the induction area, and the electrode routing layers are arranged on the first surface of the peripheral area.

The plurality of induction electrode layers are provided with a first induction electrode layer and a second induction electrode layer, the first induction electrode layer is arranged on the first surface in the induction area, the second induction electrode layer is arranged on the first induction electrode layer, and a first insulation layer is arranged between the first induction electrode layer and the second induction electrode layer.

The plurality of lead layers are arranged on the second induction electrode layer at intervals, and a second insulating layer is arranged between the plurality of lead layers and the second induction electrode layer.

The second insulating layer is provided with a plurality of grooves corresponding to the plurality of lead layers, the plurality of lead layers are accommodated in the plurality of grooves, and a micro-channel is defined between each lead layer and the inner wall corresponding to each groove.

The substrate is a glass substrate, a circuit board or a polyethylene terephthalate substrate.

At least one primer layer is formed on the plurality of sensing electrode layers by printing or plating.

The sensing electrode layers and the electrode routing layers are made of tin oxide (ITO), Indium Zinc Oxide (IZO) and Antimony Tin Oxide (ATO), and at least one lead layer is made of a high polymer material.

The induction electrode layers and the electrode routing layers are made of metal materials, and the metal materials are aluminum, gold, copper or silver materials.

The invention further provides a ribonucleic acid detection device, which comprises a control unit and a ribonucleic acid detection panel, wherein the ribonucleic acid detection panel comprises a substrate, a plurality of induction electrode layers, at least one primer layer and a plurality of electrode routing layers, the substrate is provided with a first surface and a second surface opposite to the first surface, the plurality of induction electrode layers are electrically connected with the control unit, the plurality of induction electrode layers are arranged on the first surface, the at least one primer layer is arranged on the plurality of induction electrode layers and is insulated from each other, the at least one primer layer is used for reacting with corresponding detection bodies with ribonucleic acid, the plurality of electrode routing layers are arranged on the first surface, and the plurality of electrode routing layers are electrically connected with the plurality of induction electrode layers and the control unit.

The control unit is electrically connected with the circuit board, the circuit board is provided with a display element, a processing unit, a wireless receiving and transmitting unit and a power supply unit, the processing unit is electrically connected with the control unit, the display element and the wireless receiving and transmitting unit, and the power supply unit is used for supplying power to the display element, the processing unit, the wireless receiving and transmitting unit and the ribonucleic acid detection panel.

The wireless transceiver unit comprises a Bluetooth unit, a Wi-Fi unit and an RF unit.

The display elements are a plurality of light emitting diodes or displays, and the control unit is a central processing unit, a microcontroller or a digital signal processor.

The control unit is arranged on the first surface of the substrate or the circuit board.

The second insulating layer is provided with a plurality of grooves corresponding to the plurality of lead layers, the plurality of lead layers are accommodated in the plurality of grooves of the second insulating layer, and a micro-channel is defined between each lead layer and the inner wall corresponding to each groove.

The substrate is a glass substrate, a circuit board or a polyethylene terephthalate substrate, the plurality of sensing electrode layers and the plurality of electrode routing layers are made of metal materials, the metal materials are tin oxide (ITO), Indium Zinc Oxide (IZO) or antimony oxide tin oxide (ATO), aluminum, gold, copper or silver, and at least one lead layer is made of high polymer materials.

Therefore, through the design of the invention, the detection time can be shortened, the cost can be reduced, and the user can carry out self rapid detection, so that the detection operation is simple and the detection use is very convenient.

Drawings

The accompanying drawings, which are incorporated in and constitute a part of the specification, illustrate embodiments of the invention and together with the description, serve to explain the principles of the invention. In the drawings, like reference numerals are used to indicate like elements. The drawings in the following description are directed to some, but not all embodiments of the invention. For a person skilled in the art, other figures can be derived from these figures without inventive effort.

FIG. 1 is a schematic exploded perspective view of a ribonucleic acid detection panel according to an embodiment of the present invention.

FIG. 2A schematically shows an assembled perspective view of a ribonucleic acid detection panel in an embodiment of the invention.

FIG. 2B is a schematic sectional view and a schematic partial enlarged view of a ribonucleic acid detection panel according to an embodiment of the present invention.

FIG. 2C is a schematic sectional view and a schematic partial enlarged view of an embodiment of a ribonucleic acid detection panel according to an embodiment of the present invention.

FIG. 3 is a schematic exploded perspective view of an apparatus for detecting ribonucleic acid according to another embodiment of the present invention.

FIG. 4A is a schematic perspective view of a ribonucleic acid detecting device according to another embodiment of the present invention.

Fig. 4B schematically illustrates a top view of fig. 4A in another embodiment of the present invention.

FIG. 5 is a perspective view schematically showing a second embodiment of the ribonucleic acid detecting device according to another embodiment of the present invention.

FIG. 6 is a perspective view schematically showing a third sample combination of ribonucleic acid detecting device according to another embodiment of the present invention.

Description of the reference numerals

Ribonucleic acid detection Panel 1

Substrate 11

First surface 111

Second surface 112

Sensing zone 1111

Peripheral region 1112

The first sensing electrode layer 113

First sensing electrode 1131

Second sensing electrode layer 114

Second sensing electrode 1141

Primer layer 115

Electrode wiring layer 116

First insulating layer 117

Second insulating layer 118

Groove 1181

Micro channel 1182

Ribonucleic acid detection device 2

Control unit 21

Circuit board 22

Display element 23

Processing unit 24

Wireless transceiver unit 25

Power supply unit 26

Detailed Description

In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are some embodiments of the present invention, but not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention. It should be noted that the embodiments and features of the embodiments in the present application may be arbitrarily combined with each other without conflict.

The invention provides a ribonucleic acid detection panel and a ribonucleic acid detection device. FIG. 1 is a schematic exploded perspective view of a ribonucleic acid detection panel according to an embodiment of the present invention; FIG. 2A schematically shows an assembled perspective view of a ribonucleic acid detection panel according to an embodiment of the present invention; FIG. 2B is a schematic sectional view and a schematic partial enlarged view of a ribonucleic acid detection panel according to an embodiment of the present invention; FIG. 2C is a schematic sectional view and a schematic partial enlarged view of an embodiment of a ribonucleic acid detection panel according to an embodiment of the present invention. As shown in fig. 1, 2A and 2B, a Ribonucleic acid (RNA) detection panel 1 includes a substrate 11, a plurality of sensing electrode layers, at least one primer layer 115 and a plurality of electrode routing layers 116, where the substrate 11 is a glass substrate 11, a circuit board 22 (such as a flexible circuit board) or a polyethylene terephthalate (PET) substrate 11, and the substrate 11 in this embodiment is a glass substrate 11, but not limited thereto, the substrate 11 has a first surface 111 and a second surface 112 opposite to the first surface 111, the first surface 111 of the substrate 11 is provided with a sensing region 1111 and a peripheral region 1112 surrounding the sensing region 1111, the plurality of sensing electrode layers are disposed on the first surface 111 of the sensing region 1111, the plurality of electrode routing layers 116 are disposed on the first surface 111 of the peripheral region 1112, and the plurality of electrode routing layers 116 are electrically connected to the plurality of sensing electrode layers.

The plurality of sensing electrode layers are provided with a transparent or opaque first sensing electrode layer 113 and a transparent or opaque second sensing electrode layer 114, the first sensing electrode layer 113 is provided with a plurality of first sensing electrodes 1131 (such as X-axis sensing electrodes) arranged on the first surface 111 in the sensing region 1111, the second sensing electrode layer 114 is provided with a plurality of second sensing electrodes 1141 (such as Y-axis sensing electrodes) arranged on the first sensing electrode layer 113, and the plurality of first and

second sensing electrodes

1131, 1141 are respectively represented as X-axis sensing electrodes and Y-axis sensing electrodes in this embodiment and are arranged in a staggered manner. The sensing electrode layers and the electrode routing layers 116 are made of metal, such as tin oxide (ITO), Indium Zinc Oxide (IZO), Antimony Tin Oxide (ATO), or a combination thereof, but not limited thereto, and in specific implementations, the metal may also be made of aluminum (Al), gold (Au), copper (cu), silver (Ag), or other metal (such as nickel (Ni), chromium (Cr), or an alloy thereof). In the embodiment, the form of the first and

second sensing electrodes

1131 and 1141 is a diamond shape, but not limited thereto, and in an embodiment, the form of the first and

second sensing electrodes

1131 and 1141 may be a strip, a bar, a wide strip, or a rectangle. In another embodiment, the first sensing electrodes 1131 are arranged on the first surface 111 in the sensing region 1111 in a matrix array (e.g., M × N array), the second sensing electrodes 1141 are arranged on the first sensing electrode layer 113 in a matrix array (e.g., M × N array), and the first and

second sensing electrodes

1131, 1141 are insulated from each other.

In an alternative embodiment, the plurality of first and

second sensing electrodes

1131, 1141 of the first and second sensing electrode layers 113, 114 are disposed on the first surface 111 of the substrate 11 in parallel and corresponding to each other, and the primer layer 115 is disposed on the first and second sensing electrode layers 113, 114 and is insulated from each other.

A first insulating layer 117 is disposed between the first and second sensing electrode layers 113, 114, the first insulating layer 117 is made of silicon dioxide (SiO)₂). At least one primer layer 115 is formed on the plurality of sensing electrode layers by, for example, printing or plating, and the primer layer 115 and the plurality of sensing electrode layers are insulated from each other, the primer layer 115 is, in this embodiment, a plurality of primer layers 115 are disposed on the second sensing electrode layer 114 at intervals, the primer layer 115 is made of a polymer material for interacting with a corresponding sample (e.g., saliva of a user) with ribonucleic acid (or micro ribonucleic acid micro RNA), for example, when no RNA virus in the saliva of the user (i.e., the RNA sample) reacts with the primer layer 115 not matching the corresponding specificity and cannot be combined, no electrical change (e.g., no capacitance change or dielectric coefficient change) is formed as if fingers of the user do not touch the touch sensing region 1111, so that a fixed coupling capacitance exists between the first and second sensing electrodes 1131, 1141 of the first and second sensing electrode layers 113, 114, at this time, the electric field (electric lines of force) between the first and second sensing electrodes 1131, 1141 is fixed; when the saliva (i.e., the RNA detection body) of the user contains RNA virus and the primer layer 115 with corresponding specificity reacts with each other to generate a combination with an obvious electrical change (e.g., capacitance change or dielectric coefficient change) formed on the touch-sensitive area 1111 as if the user's finger touches, for example, the primer layer 115 and the second primer layer 115A capacitance is formed between the two sensing electrode layers 114, and at this time, an electric field (electric lines) originally fixedly distributed between the first and second sensing electrodes 1131, 1141 of the first and second sensing electrode layers 113, 114 changes (i.e., the electric field changes) because a portion of the electric lines are connected to the corresponding primer layer 115, thereby changing a capacitance value of the coupling capacitance between the first and second sensing electrodes 1131, 1141 (i.e., the capacitance value changes).

In addition, the primer layer 115 is a primer that reacts with a virus (e.g., kawasaki disease, colorectal cancer, hand-foot-and-mouth disease, new coronavirus (covi-19) or other RNA-bearing virus) with a corresponding specific specificity to generate a binding, for example, a primer with a specific specificity is designed in advance to detect a virus (e.g., new coronavirus (covi-19)), if the saliva (i.e., a test sample) of a user has RNA-bearing new coronavirus (covi-19) and the primer layer 115 with a corresponding new coronavirus to generate a binding, an electrical change is obvious, and the user is indicated to confirm (e.g., positive) that the new coronavirus (covi-19) (i.e., infectious virus) is obtained; if the saliva (i.e., the test sample) of the user does not contain viruses or other viruses (e.g., Kawasaki disease), the primer layer 115 corresponding to the detected novel coronavirus does not react with each other and cannot be combined with each other, and then the electrical change is not generated, it indicates that the user has not diagnosed (e.g., is negative) the novel coronavirus (i.e., has no virus infection). Wherein the primer is a small piece of single-stranded DNA or RNA, which serves as a starting point for DNA replication, and is artificially synthesized by DNA replication (RNA primer) and Polymerase Chain Reaction (PCR) in nature.

A second insulating layer 118 disposed between the plurality of seed layers 115 and the second sensing electrode layer 114, a third insulating layer 119 disposed between the first sensing electrode layer 113 and the substrate 11, and a material of the second and third insulating

layers

118, 119 such as silicon dioxide (SiO)₂) In an alternative embodiment, the third insulating layer 119 may be omitted, such that the first sensing electrode layer 113 is disposed on the first surface 111 of the substrate 11 (e.g., a glass substrate or a PET substrate).

The plurality of primer layers 115 are, for example, long strips, located in the sensing region 1111 and arranged at intervals in the X-axis direction (or the Y-axis direction) on the second insulating layer 118 corresponding to the crossing position of the plurality of first sensing electrodes 1131 and the plurality of second sensing electrodes 1141. In an embodiment, referring to fig. 2C, a plurality of grooves 1181 are disposed on the second insulating layer 118 at positions corresponding to the plurality of primer layers 115, the plurality of primer layers 115 are accommodated in the plurality of grooves 1181 of the second insulating layer 118, a micro channel 1182 is defined between each primer layer 115 and an inner wall corresponding to each groove 1181, and the micro channel 1182 is used for collecting a detection object (such as saliva), so as to increase a contact area between the detection object and the corresponding primer layer 115, thereby effectively improving detection accuracy and shortening detection time.

Therefore, through the design of the ribonucleic acid detection panel 1 of the present invention, the detection time can be shortened, the cost can be reduced, and the user can rapidly and instantly detect the physical condition of the user through the saliva of the user, thereby not only achieving the simple detection operation, but also being very convenient in detection use.

FIG. 3 is a schematic exploded perspective view of an apparatus for detecting ribonucleic acid according to another embodiment of the present invention; FIG. 4A is a schematic perspective view of a ribonucleic acid detecting device according to another embodiment of the present invention; FIG. 4B schematically illustrates a top view of FIG. 4A in another embodiment of the present invention; FIG. 5 is a schematic perspective view of a ribonucleic acid detecting device according to another embodiment of the present invention; FIG. 6 is a perspective view schematically showing a third sample assembly of the ribonucleic acid detecting device according to another embodiment of the present invention, with reference to FIG. 1. The present embodiment mainly applies the RNA detection panel 1 of the first embodiment to the RNA detection device 2, that is, the RNA detection device 2 of the present embodiment includes the control unit 21 and the RNA detection panel 1, and the structure, connection relationship and efficacy of the RNA detection panel 1 are the same as those of the RNA detection panel 1 of the first embodiment, and thus, the description thereof is omitted. The control unit 21 is a Central Processing Unit (CPU), a Microcontroller (MCU) or a Digital Signal Processor (DSP), and the control unit 21 is electrically connected to the plurality of electrode routing layers 116 and the plurality of sensing electrode layers, the control unit 21 determines whether the detecting body and the primer layer 115 have electrical variation according to a signal of the electrical variation transmitted by the plurality of sensing electrode layers (i.e., the plurality of first and second sensing electrodes 1131, 1141), and generates a detection result, where the detection result is a result of the electrical variation (e.g., the electrical variation) between the detecting body and the primer layer 115, or a result of the electrical variation (e.g., the electrical variation) between the detecting body and the primer layer 115.

The control unit 21 in the present embodiment is configured in three modes as follows: the first aspect is as shown in fig. 3, 4A, and 4B, the control unit 21 is disposed on the first surface 111 of the peripheral region 1112 of the substrate 11 (such as the glass substrate 11 or the PET substrate 11), and the control unit 21 is bonded to the first surface 111 of the substrate 11 by a chip-on-glass (COG) bonding method, the control unit 21 is electrically connected to the circuit board 22 (such as the flexible circuit board 22, FPC), and the circuit board 22 is integrated with the substrate 11 by a thermal compression bonding method, the circuit board 22 is provided with a display element 23, a processing unit 24, a wireless transceiver unit 25, and a power supply unit 26, the processing unit 24 is electrically connected to the control unit 21, the display element 23, and the wireless transceiver unit 25, the processing unit 24 is, such as a Central Processing Unit (CPU), a Digital Signal Processor (DSP) or a controller (MCU), for processing and executing signals, for example, the processing unit 24 performs processing according to an electrical change according to a detection result transmitted by the control unit 21, and generates the detection result information, which is displayed by the display device 23 (if it shows that the virus has been diagnosed positively), if the processing unit 24 processes the detection result transmitted by the control unit 21 without electrical change and generates the detection result information, the detection result information is displayed by the display device 23 (if it shows that the virus has been diagnosed negatively), so that the user can know whether the virus has been diagnosed or not from the display device 23.

The display device 23 is shown as a display for displaying the detection result information in the present embodiment, but not limited thereto, in other embodiments, the display device 23 can be a plurality of Light Emitting Diodes (LEDs), that is, the detection result is represented by a plurality of signals, for example, a bright red LED light represents positive diagnosis, and a bright blue LED light represents negative diagnosis. The power supply unit 26 is a battery in this embodiment, and the power supply unit 26 is used to provide power to the display element 23, the processing unit 24, the wireless transceiver 25 and the ribonucleic acid detection panel 1. In one embodiment, the power supply unit 26 may be a rechargeable battery, and the circuit board 22 is provided with a connection port (e.g., a Micro USB connection port) for charging the power supply unit 26.

The wireless transceiver unit 25 is a bluetooth unit (e.g., bluetooth transceiver) in this embodiment, and the bluetooth unit is used to wirelessly connect with an electronic device (e.g., smart phone, smart watch, computer, pen or tablet computer; not shown), so that the processing unit 24 wirelessly transmits the detection result information to the electronic device through the bluetooth unit for displaying. In particular implementations, the wireless transceiver unit 25 is a Wi-Fi unit or an RF (radio frequency) unit.

When a user wants to detect kawasaki disease (or Mucocutaneous lymph gland Syndrome) virus, the user can drip his own saliva (i.e. a detected body) onto the sensing region 1111, so that the miRNA with miRNA kawasaki disease virus in the saliva is selected from miR-30e-3P and the corresponding primer layer 115 for detecting kawasaki disease virus, and after the miRNA reacts with the primer layer to generate combination, the miRNA is obviously changed in electrical property (such as capacitance change), so that the control unit 21 can determine that the detected body and the primer layer 115 are changed in electrical property according to the signals of capacitance change transmitted by the first and

second sensing electrodes

1131, 1141, and generates the detection result to be transmitted to the processing unit 24, so that the processing unit 24 processes the electrical change according to the detection result, and transmits the detection result information to the display device 23, so that the user can know the detection result information displayed by the display device 23 (see Table 1). If the miRNA in the saliva of the user is miR-223-3P (without miRNA kawasaki disease virus) and the primer layer 115 corresponding to detecting kawasaki disease virus is unable to react with each other, the processing unit 24 processes the detection result transmitted by the control unit 21 as the non-electrical change, and transmits the detection result information, so that the user can obtain the detection result information (as shown in table 1) displayed by the display element 23. Wherein, as shown in table 1, the graph of the detection results of the detection bodies and the corresponding primer layers is shown, the detection result value (e.g. 32.5) corresponding to the detection body miR-30e-3P in the graph is greater than the detection result value (e.g. 6.2) corresponding to the detection body miR-223-3P, the preset reference value of the detection result value in this embodiment is, for example, 15, that is, the detection result value is equal to or greater than 15, which indicates that the diagnosis is confirmed, and the larger the detection result value is, which indicates that the number of days with virus in the body of the user is longer, and the more serious the symptoms are, and if the detection result value is less than 15.

Table 1 table of detection results of detectors and corresponding primer layers according to another embodiment of the present invention

Therefore, the ribonucleic acid detection device 2 of the present invention is designed to detect diseases by electrical measurement, so that the detection time can be effectively shortened, such as 15 minutes, to detect diseases (such as disease virus) and reduce the cost, and any user can perform real-time detection at home, thereby achieving simple detection operation and convenient detection.

Finally, it should be noted that: in this document, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, an element defined by the phrase "comprising an …" does not exclude the presence of other identical elements in a process, method, article, or apparatus that comprises the element.

The above examples are only for illustrating the technical solutions of the present invention, and are not limited thereto. Although the present invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some technical features may be equivalently replaced; and such modifications or substitutions do not depart from the spirit and scope of the corresponding technical solutions of the embodiments of the present invention.

Claims

1. A panel for detecting ribonucleic acid, comprising:

a substrate having a first surface and a second surface opposite the first surface;

a plurality of sensing electrode layers disposed on the first surface;

at least one primer layer, which is arranged on the plurality of sensing electrode layers and insulated from each other, and is used for reacting with a corresponding detection body with ribonucleic acid; and

and the electrode routing layers are arranged on the first surface and are electrically connected with the induction electrode layers.

2. The panel of claim 1, wherein the first surface of the substrate has a sensing area and a peripheral area surrounding the sensing area, the plurality of sensing electrode layers are disposed on the first surface of the sensing area, and the plurality of electrode traces are disposed on the first surface of the peripheral area.

3. The panel of claim 2, wherein the plurality of sensing electrode layers comprises a first sensing electrode layer and a second sensing electrode layer, the first sensing electrode layer is disposed on the first surface in the sensing region, the second sensing electrode layer is disposed on the first sensing electrode layer, and a first insulating layer is disposed between the first and second sensing electrode layers.

4. The panel of claim 3, wherein the plurality of primer layers are disposed on the second sensing electrode layer at intervals, and a second insulating layer is disposed between the plurality of primer layers and the second sensing electrode layer.

5. The panel of claim 4, wherein a plurality of grooves are formed in the second insulating layer at positions corresponding to the plurality of primer layers, the plurality of primer layers are accommodated in the plurality of grooves, and a micro-channel is defined between each primer layer and an inner wall of each groove.

6. The panel of claim 1, wherein the substrate is a glass substrate, a circuit board, or a polyethylene terephthalate substrate.

7. The panel of claim 1, wherein the at least one primer layer is formed on the plurality of sensing electrode layers by printing or plating.

8. The panel of claim 1, wherein the sensing electrode layers and the electrode routing layers are made of tin oxide (ITO), Indium Zinc Oxide (IZO), or Antimony Tin Oxide (ATO), and the at least one primer layer is made of a polymer material.

9. The panel of claim 1, wherein the sensing electrode layers and the electrode traces are made of metal, and the metal is aluminum, gold, copper or silver.

10. A ribonucleic acid detection device, comprising:

a control unit; and

a panel for ribonucleic acid detection, comprising:

a plurality of sensing electrode layers electrically connected to the control unit, the plurality of sensing electrode layers being disposed on the first surface;

and the electrode routing layers are arranged on the first surface and are electrically connected with the sensing electrode layers and the control unit.

11. The apparatus according to claim 10, wherein the control unit is electrically connected to a circuit board, the circuit board is provided with a display device, a processing unit, a wireless transceiver unit and a power supply unit, the processing unit is electrically connected to the control unit, the display device and the wireless transceiver unit, and the power supply unit is used to provide power to the display device, the processing unit, the wireless transceiver unit and the ribonucleic acid detection panel.

12. The apparatus according to claim 11, wherein the wireless transceiver is a Bluetooth unit, a Wi-Fi unit, and an RF unit.

13. The apparatus according to claim 11, wherein the display device is a plurality of light emitting diodes or a display, and the control unit is a central processing unit, a microcontroller or a digital signal processor.

14. The apparatus according to claim 11, wherein the control unit is disposed on the first surface of the substrate or on the circuit board.

15. The apparatus according to claim 10, wherein the first surface of the substrate has a sensing area and a peripheral area surrounding the sensing area, the plurality of sensing electrode layers are disposed on the first surface of the sensing area, and the plurality of electrode routing layers are disposed on the first surface of the peripheral area.

16. The apparatus according to claim 15, wherein the plurality of sensing electrode layers comprises a first sensing electrode layer and a second sensing electrode layer, the first sensing electrode layer is disposed on the first surface in the sensing region, the second sensing electrode layer is disposed on the first sensing electrode layer, and a first insulating layer is disposed between the first and second sensing electrode layers.

17. The apparatus according to claim 16, wherein the primer layers are disposed on the second sensing electrode layer at intervals, and a second insulating layer is disposed between the primer layers and the second sensing electrode layer.

18. The apparatus according to claim 17, wherein the second insulating layer has a plurality of grooves corresponding to the plurality of primer layers, the primer layers are received in the grooves of the second insulating layer, and a micro-channel is defined between each primer layer and an inner wall of each groove.

19. The apparatus of claim 10, wherein the substrate is a glass substrate, a circuit board or a polyethylene terephthalate substrate, the sensing electrode layers and the electrode routing layers are made of a metal material, the metal material Is Tin Oxide (ITO), Indium Zinc Oxide (IZO) or Antimony Tin Oxide (ATO), aluminum, gold, copper or silver, and the at least one primer layer is made of a polymer material.

20. The apparatus according to claim 10, wherein the at least one primer layer is formed on the plurality of sensing electrode layers by printing or plating.