CN111529572A - Scutellaria baicalensis extract self-microemulsion with biological antibacterial activity - Google Patents
Scutellaria baicalensis extract self-microemulsion with biological antibacterial activity Download PDFInfo
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- CN111529572A CN111529572A CN202010561960.0A CN202010561960A CN111529572A CN 111529572 A CN111529572 A CN 111529572A CN 202010561960 A CN202010561960 A CN 202010561960A CN 111529572 A CN111529572 A CN 111529572A
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/53—Lamiaceae or Labiatae (Mint family), e.g. thyme, rosemary or lavender
- A61K36/539—Scutellaria (skullcap)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/06—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite
- A61K47/08—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite containing oxygen, e.g. ethers, acetals, ketones, quinones, aldehydes, peroxides
- A61K47/10—Alcohols; Phenols; Salts thereof, e.g. glycerol; Polyethylene glycols [PEG]; Poloxamers; PEG/POE alkyl ethers
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
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- A61K47/06—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite
- A61K47/26—Carbohydrates, e.g. sugar alcohols, amino sugars, nucleic acids, mono-, di- or oligo-saccharides; Derivatives thereof, e.g. polysorbates, sorbitan fatty acid esters or glycyrrhizin
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- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/44—Oils, fats or waxes according to two or more groups of A61K47/02-A61K47/42; Natural or modified natural oils, fats or waxes, e.g. castor oil, polyethoxylated castor oil, montan wax, lignite, shellac, rosin, beeswax or lanolin
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/10—Dispersions; Emulsions
- A61K9/107—Emulsions ; Emulsion preconcentrates; Micelles
- A61K9/1075—Microemulsions or submicron emulsions; Preconcentrates or solids thereof; Micelles, e.g. made of phospholipids or block copolymers
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- A61K2236/30—Extraction of the material
- A61K2236/37—Extraction at elevated pressure or temperature, e.g. pressurized solvent extraction [PSE], supercritical carbon dioxide extraction or subcritical water extraction
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Abstract
The invention discloses a scutellaria baicalensis extract self-microemulsion with biological antibacterial activity, which is prepared by using 2-6% of scutellaria baicalensis CO2The supercritical extract is active substance, and is mixed with 10-40% of nonionic surfactant, 20-50% of short-chain fatty alcohol and 20-55% of modified plantThe oil is mixed to form the self-microemulsion which is easy to form a thermodynamically stable microemulsion system with water. The self-microemulsion can be used as a livestock and poultry sterilizing bacteriostatic agent and a livestock and poultry growth promoter after being diluted by water, and is used for promoting the growth of livestock and poultry and improving the immunity.
Description
Technical Field
The invention belongs to the technical field of veterinary drugs, and relates to a self-microemulsion with biological antibacterial property based on active substance components of scutellaria baicalensis extracts.
Background
The long-term use of antibiotics has led to the emergence of widely drug-resistant germs. The development of novel antibacterial agents which have high safety, high antibacterial activity and difficult drug resistance is a problem to be solved urgently in the current livestock and poultry breeding process.
The Chinese herbal medicine has been applied to livestock and poultry breeding because of small toxic and side effects, no residue, difficult generation of drug resistance and medicinal and nutritional effects.
Baikal skullcap root (Scutellaria baicalensisGeorgi), derived from dried root of Scutellaria baicalensis Georgi of Labiatae, has antibacterial, hepatoprotective, antiviral, antioxidant, free radical scavenging, antiallergic, and neuron protecting effects. The scutellaria is a traditional Chinese medicine material which is proved to have good antibacterial effect, and the extracts of the scutellaria, wogonoside, baicalein and wogonin have the functions of inhibiting bacteria, resisting bacteria and enhancing the immunity of livestock and poultry. A large number of researches show that baicalein and wogonin are substances which really play a role in drug action in scutellaria baicalensis, and baicalin and wogonoside also need to be hydrolyzed into aglycon baicalein and wogonin by intestinal flora to play roles.
However, baicalein and wogonin are fat-soluble compounds with strong hydrophobicity, and common medicinal solvents such as polyol and water cannot effectively dissolve baicalein and wogonin, so that a good bacteriostatic effect cannot be achieved, and the baicalein and wogonin cannot be added into drinking water of livestock and poultry to promote the healthy growth of the livestock and poultry. However, the direct use of baicalein and wogonin has the problems of easy oxidation and inactivation, poor solubility in water, ineffective dispersion and the like, thereby affecting the drug effect.
Disclosure of Invention
The invention aims to overcome the defects in the prior art and provide the scutellaria baicalensis extract self-microemulsion which is easy to form a thermodynamically stable microemulsion system with water and has biological antibacterial property.
The scutellaria baicalensis extract self-microemulsion is prepared by using scutellaria baicalensis CO2The supercritical extract is active substance, and is mixed with nonionic surfactant, short-chain fatty alcohol and modified vegetable oil to form a self-microemulsion with biological antibacterial activity.
Specifically, the self-microemulsion is prepared from a nonionic surfactant, modified vegetable oil, short-chain fatty alcohol and scutellaria baicalensis CO2The supercritical extract is prepared by mixing the following components in percentage by mass: 10-40% of nonionic surfactant, 20-55% of modified vegetable oil, 20-50% of short-chain fatty alcohol, and 20-50% of scutellaria baicalensis CO22-6% of supercritical extract.
The invention uses the nonionic surfactant, short-chain fatty alcohol, modified vegetable oil and scutellaria baicalensis CO2The reasonable combination of the supercritical extracts forms the self-microemulsion with biological antibacterial property. Diluting the self-microemulsion with water, and adding radix Scutellariae CO2The supercritical extract will be present in the dilution in a dispersed state of less than 100 nm. When the microemulsion is diluted by water, the microemulsion can form a stable microemulsion system with water, and the diluted solution has clear and transparent appearance, no layering and no precipitation.
Wherein, the scutellaria CO2The supercritical extract is prepared from conventional CO2The supercritical extraction method is used for extracting Scutellariae radix to obtain substance containing baicalein and wogonin as main ingredients.
Specifically, the nonionic surfactant in the self-microemulsion is a mixture consisting of span-80 and tween-60 and/or tween-20.
More specifically, the modified vegetable oil is polyoxyethylene castor oil, and isopropyl laurate and/or isopropyl myristate are/is added into the polyoxyethylene castor oil.
Further, the short-chain fatty alcohol is one or more of isopropanol, glycerol and glycol.
The self-microemulsion with biological bacteriostasis can be prepared by the following method: mixing nonionic surfactant, modified vegetable oil, short chain fatty alcohol and Scutellariae radix CO2Adding the supercritical extract into a stirring kettle according to the mass percentage, stirring and mixing until the supercritical extract is transparentHomogeneous state, prepared from microemulsion.
The self-microemulsion with biological bacteriostasis prepared by the invention is diluted by water and can be used after being uniformly stirred. The particle size of dispersed phase droplets of the self-microemulsion after being diluted by water is less than 100 nm.
The self-microemulsion can be used as a disinfectant and bacteriostatic agent for livestock and poultry. When the antibacterial agent is used for bacteriostasis, water is added according to the mass ratio and is diluted by 50-100 times for use.
The self-microemulsion with biological bacteriostasis can also be used as a livestock and poultry growth promoter to promote the growth of livestock and poultry and improve the immunity. When the compound is used as a growth promoter for livestock and poultry, 100-500 times of water is added according to the mass ratio for dilution.
The invention adopts the self-microemulsion technology to prepare the scutellaria baicalensis extract self-microemulsion so that the scutellaria baicalensis CO is in a state of being CO2The supercritical extract can be dispersed in water at a state of less than 100nm after being diluted with water to form a thermodynamically stable system with uniform and transparent appearance, no delamination and no precipitation, thereby solving the problem of CO in Scutellariae radix2The supercritical extract is difficult to use directly, and the drug effect is obviously improved.
Bacteriostasis evaluation tests carried out on escherichia coli show that the minimum bacteriostasis concentration of the self-microemulsion is only 1/53 of the concentration of the traditional scutellaria baicalensis water extract (main components baicalin and wogonoside). The bacteriostatic evaluation test of staphylococcus aureus also shows that the minimum bacteriostatic concentration of the self-microemulsion is only 1/16 of the concentration of the traditional scutellaria baicalensis water extract.
Growth experiments and immune organ index experimental evaluation of 42-day-old broilers show that compared with a blank group, the self-microemulsion can improve the average body weight of 42-day-old broilers by over 10 percent, improves the thymus index, the spleen index and the bursal index by over 5 percent, 30 percent and 11 percent respectively, and obviously improves the whole body immune level of animals.
Drawings
FIG. 1 is a graph of the effect of Scutellaria baicalensis Georgi extract self-microemulsions on E.coli growth curves.
FIG. 2 is the effect of aqueous extracts of Scutellaria baicalensis on the growth curve of E.coli.
Figure 3 is the effect of scutellaria baicalensis extract self-microemulsions on the growth curve of staphylococcus aureus.
FIG. 4 is the effect of aqueous extracts of Scutellaria on the growth curve of Staphylococcus aureus.
Detailed Description
The following examples further describe embodiments of the present invention. The following examples are only for illustrating the technical solutions of the present invention more clearly, and do not limit the scope of the present invention. Various changes, modifications, substitutions and alterations to these embodiments will be apparent to those skilled in the art without departing from the principles and spirit of this invention.
Example 1.
Adding 10g span-80 and 4g tween-20 into 50g polyoxyethylene castor oil, stirring, mixing, adding 30g isopropanol, stirring, mixing, adding 6g Scutellariae radix CO2And (3) stirring and mixing the supercritical extract until the appearance is clear and transparent, and preparing the self-microemulsion with biological antibacterial activity.
Example 2.
Adding 10g isopropyl laurate and 15g isopropyl myristate into 30g polyoxyethylene castor oil, stirring, mixing, adding 6g span-80, 2g tween-20 and 2g tween-60, stirring, mixing, adding 20g isopropanol and 10g glycerol, stirring, mixing, adding 5g Scutellariae radix CO2And (3) stirring and mixing the supercritical extract until the appearance is clear and transparent, and preparing the self-microemulsion with biological antibacterial activity.
Example 3.
Adding 14g isopropyl laurate into 14g polyoxyethylene castor oil, stirring and mixing uniformly, adding 6g span-80 and 14g tween-60, stirring and mixing uniformly, adding 20g isopropanol, 20g glycerol and 10g ethylene glycol, stirring and mixing uniformly, adding 2g scutellaria CO2And (3) stirring and mixing the supercritical extract until the appearance is clear and transparent, and preparing the self-microemulsion with biological antibacterial activity.
Example 4.
Adding 22 g of polyoxyethylene castor oilg isopropyl myristate, stirring, mixing, adding span-80 15g, Tween-20 15g, Tween-60 10g, stirring, mixing, adding glycerol 20g, stirring, mixing, adding Scutellariae radix CO 3g2And (3) stirring and mixing the supercritical extract until the appearance is clear and transparent, and preparing the self-microemulsion with biological antibacterial activity.
Example 5.
Adding 10g isopropyl myristate into 10g polyoxyethylene castor oil, stirring, mixing, adding 14g span-80, 12g tween-20, 12g tween-60, stirring, mixing, adding 20g glycerol and 20g ethylene glycol, stirring, mixing, adding 3g Scutellariae radix CO2And (3) stirring and mixing the supercritical extract until the appearance is clear and transparent, and preparing the self-microemulsion with biological antibacterial activity.
Example 6.
Adding 10g isopropyl myristate into 10g polyoxyethylene castor oil, stirring, mixing, adding 14g span-80, 12g tween-20 and 12g tween-60, stirring, mixing, adding 40g ethylene glycol, stirring, mixing, adding 3g Scutellariae radix CO2And (3) stirring and mixing the supercritical extract until the appearance is clear and transparent, and preparing the self-microemulsion with biological antibacterial activity.
Example 7.
Adding 26g isopropyl laurate into 20g polyoxyethylene castor oil, stirring and mixing uniformly, adding 10g span-80 and 12g tween-20, stirring and mixing uniformly, adding 20g isopropanol and 10g ethylene glycol, stirring and mixing uniformly, adding 2g scutellaria CO2And (3) stirring and mixing the supercritical extract until the appearance is clear and transparent, and preparing the self-microemulsion with biological antibacterial activity.
Example 1 is applied.
The scutellaria baicalensis extract self-microemulsion prepared in the above examples 1 to 7 was diluted by 50 times and 500 times with water, respectively, and the average particle size of the diluted solution was measured by a dynamic light scattering method using a Nano ZS dynamic light scattering instrument of Malvern, and the measurement results are specifically shown in table 1.
As can be seen from Table 1, the particle size of the microemulsion of each example is below 100nm after being diluted with water, and is within the range of particle size of the microemulsion (10-100 nm), which indicates that the microemulsion is still uniformly dispersed after being diluted, and the appearance of the microemulsion is similar to that of a transparent or slightly transparent liquid.
Example 2 is applied.
The diameter of the inhibition zone of the scutellaria baicalensis extract self-microemulsion prepared in examples 1-7 on escherichia coli and staphylococcus aureus is measured by adopting a filter paper sheet method, and the antibacterial activity of the scutellaria baicalensis extract self-microemulsion is examined.
Preparing a solid culture medium: weighing 1.0g of peptone, 0.5g of beef extract, 0.5g of sodium chloride and about 2.0g of agar powder, adding water, heating for dissolving, fixing the volume to 100mL, adjusting the pH value to = 7.2-7.4 by using a 10% NaOH solution, filtering, subpackaging into triangular bottles, placing into a high-pressure steam kettle at 121 ℃ and 120Pa, and sterilizing for 20min for later use.
Preparing a liquid culture medium: except that agar powder is not added, the preparation method is the same as that of the solid culture medium.
Activating strains: inoculating the strain onto a slant culture medium on an aseptic operation table, placing the slant culture medium in an incubator, and performing slant culture at 37 ℃ for 18-24 hours.
Preparing a bacterial suspension: selecting typical bacterial colony after strain activation culture, inoculating into sterilized liquid culture medium, culturing in 37 deg.C incubator for 24 hr, and diluting with sterile normal saline to 10%6CFU/mL is ready for use.
And under an aseptic environment, pouring the sterilized solid culture medium into a culture dish sterilized by dry heat, wherein the thickness of the culture medium is not more than 2/3 of the culture dish, after the culture medium is solidified, respectively sucking 200 mu L of escherichia coli and staphylococcus aureus suspension onto the culture medium by using a liquid transfer gun, marking, and uniformly smearing by using a coating rod.
Respectively putting the sterilized filter paper sheets into the self-microemulsion of the scutellaria baicalensis extract prepared in the embodiment 1-7, soaking for 20min, taking out the filter paper sheets permeated with the self-microemulsion, and tightly pasting the filter paper sheets on a solid culture medium. And blank self-microemulsion was used as a control.
And (3) placing the culture medium in an incubator at 37 ℃ for culturing for 24h, observing the bacteriostatic effect, determining the diameter of a bacteriostatic zone, and repeating each sample for 3 times. The results are shown in Table 2.
As can be seen from Table 2, the Scutellaria baicalensis extract self-microemulsion prepared in examples 1-7 has a good bacteriostatic effect on Escherichia coli and Staphylococcus aureus. Scutellaria baicalensis CO2The higher the concentration of the supercritical extract is, the larger the diameter of the inhibition zone is, and the stronger the inhibition capacity is.
Example 3 is applied.
Preparing a scutellaria aqueous extract according to a conventional method: taking radix scutellariae medicinal material powder, taking water as a solvent, carrying out reflux extraction for 3 hours at a material-liquid ratio of 1:10, filtering, concentrating the filtrate under reduced pressure, and drying to obtain a brown radix scutellariae aqueous extract.
The above-mentioned aqueous extract of scutellaria and the self-microemulsion of scutellaria extract of example 1 were used for the determination of the minimum inhibitory concentration performance, respectively.
Escherichia coli and staphylococcus aureus are used as test bacteria, and bacterial suspension is prepared.
Preparing liquid culture medium corresponding to test strain, respectively placing 100mL into 3 250mL conical flasks labeled as No. 1, No. 2 and No. 3, autoclaving at 121 deg.C for 20min, cooling, and inoculating 1mL of appropriate concentration (10%5CFU/mL).
Adding appropriate amount of Scutellariae radix extract self-microemulsion or Scutellariae radix water extract solution into No. 1 and No. 2 conical flask respectively to make final concentration reach 1 × MIC and 1/2 × MIC, and taking No. 3 normal strain as control.
The Erlenmeyer flask was placed at 37 ℃ and shake-cultured at 150r/min for 24h, during which time samples were taken at 2h intervals under sterile conditions, and the absorbance OD values were determined and recorded at a wavelength of 600nm, and repeated 3 times. And (3) drawing a growth curve of the test bacteria by taking the time as an abscissa and the OD average value as an ordinate, wherein the specific growth curve result is shown in the figures 1-4.
As can be seen from figures 1-4, the scutellaria baicalensis extract self-microemulsion has a strong inhibition effect on escherichia coli and staphylococcus aureus, the MIC value on escherichia coli can reach 0.375mg/mL, and the MIC value on staphylococcus aureus can reach 0.187 mg/mL. And the MIC values of the scutellaria aqueous extract to escherichia coli and staphylococcus aureus are only 20mg/mL and 3mg/mL respectively.
Therefore, the inhibition effect and the bacteriostatic effect of the scutellaria baicalensis extract self-microemulsion on escherichia coli and staphylococcus aureus are obviously better than those of the scutellaria baicalensis aqueous extract. The minimum inhibitory concentration to Escherichia coli is 1/53 of traditional Scutellariae radix water extract (main components baicalin and wogonoside); the minimum inhibitory concentration to staphylococcus aureus is only 1/16 of the concentration of the traditional scutellaria baicalensis water extract.
Example 4 is applied.
The white feather broiler is taken as an experimental object, and the growth experiment and the whole body immunity evaluation result after the broiler feeding water is added with a small amount of the scutellaria baicalensis extract self-microemulsion prepared in the examples 1-4 are examined.
Selecting 350 feathers of healthy white feather broilers of 1 day old and close weight, randomly dividing the feathers into a blank group, 4 scutellaria baicalensis extract self-microemulsion experimental groups (experiment 1 group, experiment 2 group, experiment 3 group and experiment 4 group), 7 scutellaria baicalensis extract self-microemulsion control groups (control 1 group) and scutellaria baicalensis aqueous extract control groups (control 2 group), and repeating 50 in each group.
The blank group was fed normally a basal diet without antibiotics and given tap water for drinking.
The 4 scutellaria baicalensis extract self-microemulsion experimental groups are respectively diluted by respective self-microemulsions by 200 times on the basis of normal daily ration without antibiotics, and are fed with drinking water.
Radix Scutellariae extract self-microemulsion control group containing radix Scutellariae CO2The supercritical extract was added in an amount of 0.03%, and the scutellaria baicalensis extract self-microemulsion of example 1 was mixed with a basal diet without antibiotics to feed, and tap water was given.
The control group of the scutellaria aqueous extract is fed with water with the content of the scutellaria aqueous extract of 0.03 percent on the basis of normal daily ration without antibiotics.
The breeding period is 42 days. After the feeding is finished, compared with a blank group, the influence of different feeding modes of the scutellaria baicalensis extract self-microemulsion and the influence of the scutellaria baicalensis aqueous extract on the growth and immune organ index of the white feather broiler is evaluated, and specific comparison results are shown in table 3.
Weight gain rate = (average body weight of experimental group or control group-average body weight of blank group)/(average body weight of blank group) × 100%.
Thymus, spleen and bursa of fabricius index increase rate = (mean index of experimental group or control group-mean index of blank group)/(mean index of blank group) × 100%.
The results in table 3 show that when the self-microemulsion of the present invention is added to drinking water for feeding, the average body weight of 42-day-old broiler chickens can be increased by more than 10% and the maximum increase is close to 20% compared with the blank group; the thymus, spleen and bursal index of 42-day-old broiler chickens are respectively improved by more than 5%, 30% and 11%, and the whole body immunity level of animals is obviously improved.
The same self-microemulsion is mixed in the basic ration for direct feeding, although the weight and the immune organ index of the broiler chicken can be improved, the effect is obviously worse compared with the drinking water adding mode with the same concentration, which indicates that the bioavailability of the drug of the broiler chicken is not as good as that of the broiler chicken by the feeding mode of mixing the self-microemulsion in the basic ration.
Meanwhile, the scutellaria baicalensis aqueous extract is added into drinking water for feeding, the weight and immune organ index of the broiler chicken are basically the same as those of a blank group, so that the bioavailability of the scutellaria baicalensis aqueous extract is lower, and the difference is obvious compared with that of the self-microemulsion with the same concentration.
The average daily gain of the livestock and poultry is an important index reflecting the effectiveness of the additive. The experimental results show that the average daily gain of the broiler chicken can be remarkably increased and the economic benefit of chicken raising is greatly increased by adding a small amount of the scutellaria baicalensis extract self-microemulsion into the broiler chicken feeding water.
The development condition and the function of the animal immune organ directly determine the whole body immune level of the poultry, namely the quality of the immune organ can be used for evaluating the immune state of the chicken. The scutellaria baicalensis extract self-microemulsion can improve thymus, spleen and bursal disease index of broiler chicken and greatly improve the whole body immunity level of animals. The scutellaria baicalensis extract self-microemulsion is dispersed in a nanometer level, is favorable for the absorption of the medicine in the body, and verifies that the bioavailability of the baicalein extracted from the scutellaria baicalensis extract self-microemulsion is much higher than that of the baicalin.
Claims (9)
1. A biological antibacterial self-microemulsion of Scutellariae radix extract is prepared from nonionic surfactant, modified vegetable oil, short chain fatty alcohol and Scutellariae radix CO2The supercritical extract is prepared by mixing the following components in percentage by mass: 10-40% of nonionic surfactant, 20-55% of modified vegetable oil, 20-50% of short-chain fatty alcohol, and 20-50% of scutellaria baicalensis CO22-6% of supercritical extract.
2. The self-microemulsion of scutellaria baicalensis extract according to claim 1, wherein the non-ionic surfactant is a mixture of span-80 and tween-60 and/or tween-20.
3. The self-microemulsion of scutellaria baicalensis extract according to claim 1, wherein the modified vegetable oil is a polyoxyethylene castor oil to which isopropyl laurate and/or isopropyl myristate are added.
4. The self-microemulsion of scutellaria baicalensis extract according to claim 1, wherein the short-chain fatty alcohol is one or more of isopropanol, glycerol and ethylene glycol.
5. The method for preparing the scutellaria baicalensis extract self-microemulsion according to claim 1, which comprises mixing the non-ionic surfactant, the modified vegetable oil, the short-chain fatty alcohol and the scutellaria baicalensis CO2And putting the supercritical extract into a stirring kettle according to the mass percentage, stirring and mixing to a transparent and uniform state, and preparing the self-microemulsion.
6. The use of the scutellaria baicalensis extract self-microemulsion as a bacteriostatic agent for livestock and poultry.
7. The use of claim 6, wherein the scutellaria baicalensis extract self-microemulsion is diluted with 50-100 times of water according to the mass ratio.
8. The use of the scutellaria baicalensis extract self-microemulsion according to claim 1 as a livestock and poultry growth promoter.
9. The use of claim 8, wherein the scutellaria baicalensis extract self-microemulsion is diluted with 100-500 times of water according to the mass ratio.
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Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101088524A (en) * | 2006-06-12 | 2007-12-19 | 大百汇生物科技（深圳）有限公司 | Phosphatide composition of active skull cap components and its prepn process and prepn |
| CN103110618A (en) * | 2013-02-07 | 2013-05-22 | 吴一心 | Medical application of wogonin |
| CN103315960A (en) * | 2012-03-19 | 2013-09-25 | 胡容峰 | Solid self-microemulsion based on spherical crystallization technique and preparation method thereof |
| CN103830176A (en) * | 2012-11-26 | 2014-06-04 | 青岛百草汇中草药研究所 | Baicalin emulsion and preparation method thereof |
| CN107213146A (en) * | 2017-06-02 | 2017-09-29 | 广州中医药大学 | Application of the wogonin in treatment colon cancer drug is prepared |
| CN108175744A (en) * | 2018-03-15 | 2018-06-19 | 武汉轻工大学 | A kind of scutelloside microemulsion solution and scutelloside micro emulsion gels and its preparation method and application |
| CN109553598A (en) * | 2017-09-26 | 2019-04-02 | 任肖雄 | The method of baicalein is obtained in a kind of scutellariae,radix |
-
2020
- 2020-06-18 CN CN202010561960.0A patent/CN111529572B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101088524A (en) * | 2006-06-12 | 2007-12-19 | 大百汇生物科技（深圳）有限公司 | Phosphatide composition of active skull cap components and its prepn process and prepn |
| CN103315960A (en) * | 2012-03-19 | 2013-09-25 | 胡容峰 | Solid self-microemulsion based on spherical crystallization technique and preparation method thereof |
| CN103830176A (en) * | 2012-11-26 | 2014-06-04 | 青岛百草汇中草药研究所 | Baicalin emulsion and preparation method thereof |
| CN103110618A (en) * | 2013-02-07 | 2013-05-22 | 吴一心 | Medical application of wogonin |
| CN107213146A (en) * | 2017-06-02 | 2017-09-29 | 广州中医药大学 | Application of the wogonin in treatment colon cancer drug is prepared |
| CN109553598A (en) * | 2017-09-26 | 2019-04-02 | 任肖雄 | The method of baicalein is obtained in a kind of scutellariae,radix |
| CN108175744A (en) * | 2018-03-15 | 2018-06-19 | 武汉轻工大学 | A kind of scutelloside microemulsion solution and scutelloside micro emulsion gels and its preparation method and application |
Non-Patent Citations (3)
| Title |
|---|
| 刘艳平: "自微乳释药***在提高中药难溶性药物生物利用度中的应用", 《中国医药导报》 * |
| 李景松 等: "黄芩药材中活性成分黄芩苷的研究概况", 《世界中医药》 * |
| 柯学 等: "黄芩素自微乳的制备及大鼠体内生物利用度研究", 《中国新药杂志》 * |
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