CN111504739A - Immunohistochemical slide glass for detection - Google Patents
Immunohistochemical slide glass for detection Download PDFInfo
- Publication number
- CN111504739A CN111504739A CN202010318770.6A CN202010318770A CN111504739A CN 111504739 A CN111504739 A CN 111504739A CN 202010318770 A CN202010318770 A CN 202010318770A CN 111504739 A CN111504739 A CN 111504739A
- Authority
- CN
- China
- Prior art keywords
- positive
- slide
- tissue
- area
- control
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000011521 glass Substances 0.000 title claims abstract description 36
- 238000001514 detection method Methods 0.000 title claims abstract description 18
- 230000002055 immunohistochemical effect Effects 0.000 title description 6
- 239000013641 positive control Substances 0.000 claims abstract description 32
- 238000013115 immunohistochemical detection Methods 0.000 claims abstract description 27
- 239000000758 substrate Substances 0.000 claims abstract description 18
- 238000000034 method Methods 0.000 claims abstract description 17
- 239000000427 antigen Substances 0.000 claims abstract description 11
- 108091007433 antigens Proteins 0.000 claims abstract description 11
- 102000036639 antigens Human genes 0.000 claims abstract description 11
- 241000711573 Coronaviridae Species 0.000 claims abstract description 7
- 208000025721 COVID-19 Diseases 0.000 claims abstract description 4
- 239000000126 substance Substances 0.000 claims description 21
- 238000006731 degradation reaction Methods 0.000 claims description 13
- 239000010410 layer Substances 0.000 claims description 12
- 230000015556 catabolic process Effects 0.000 claims description 9
- 238000000576 coating method Methods 0.000 claims description 9
- 239000011248 coating agent Substances 0.000 claims description 8
- 238000005260 corrosion Methods 0.000 claims description 7
- 230000002265 prevention Effects 0.000 claims description 7
- 239000000853 adhesive Substances 0.000 claims description 5
- 230000001070 adhesive effect Effects 0.000 claims description 5
- 239000012790 adhesive layer Substances 0.000 claims description 5
- 230000007797 corrosion Effects 0.000 claims description 5
- 238000007747 plating Methods 0.000 claims description 3
- 229920000729 poly(L-lysine) polymer Polymers 0.000 claims description 3
- 108010010803 Gelatin Proteins 0.000 claims description 2
- 229920000159 gelatin Polymers 0.000 claims description 2
- 239000008273 gelatin Substances 0.000 claims description 2
- 235000019322 gelatine Nutrition 0.000 claims description 2
- 235000011852 gelatine desserts Nutrition 0.000 claims description 2
- 239000012188 paraffin wax Substances 0.000 claims description 2
- 230000008569 process Effects 0.000 abstract description 6
- 238000003326 Quality management system Methods 0.000 abstract description 2
- 230000000593 degrading effect Effects 0.000 abstract description 2
- 238000004043 dyeing Methods 0.000 abstract description 2
- 230000014759 maintenance of location Effects 0.000 abstract description 2
- 210000001519 tissue Anatomy 0.000 description 75
- 239000007853 buffer solution Substances 0.000 description 13
- 238000002791 soaking Methods 0.000 description 13
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 10
- 239000001993 wax Substances 0.000 description 7
- WZUVPPKBWHMQCE-UHFFFAOYSA-N Haematoxylin Chemical compound C12=CC(O)=C(O)C=C2CC2(O)C1C1=CC=C(O)C(O)=C1OC2 WZUVPPKBWHMQCE-UHFFFAOYSA-N 0.000 description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 6
- 241000700605 Viruses Species 0.000 description 4
- 230000002745 absorbent Effects 0.000 description 4
- 239000002250 absorbent Substances 0.000 description 4
- 239000007788 liquid Substances 0.000 description 4
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 3
- 210000004027 cell Anatomy 0.000 description 3
- 238000000605 extraction Methods 0.000 description 3
- 210000004072 lung Anatomy 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 238000010186 staining Methods 0.000 description 3
- 239000008399 tap water Substances 0.000 description 3
- 235000020679 tap water Nutrition 0.000 description 3
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 2
- CTQNGGLPUBDAKN-UHFFFAOYSA-N O-Xylene Chemical compound CC1=CC=CC=C1C CTQNGGLPUBDAKN-UHFFFAOYSA-N 0.000 description 2
- 229920002334 Spandex Polymers 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 238000009835 boiling Methods 0.000 description 2
- 210000005013 brain tissue Anatomy 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 239000012153 distilled water Substances 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 238000011065 in-situ storage Methods 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000003908 quality control method Methods 0.000 description 2
- 230000035945 sensitivity Effects 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 239000004759 spandex Substances 0.000 description 2
- 239000008096 xylene Substances 0.000 description 2
- WYTZZXDRDKSJID-UHFFFAOYSA-N (3-aminopropyl)triethoxysilane Chemical compound CCO[Si](OCC)(OCC)CCCN WYTZZXDRDKSJID-UHFFFAOYSA-N 0.000 description 1
- 241000251468 Actinopterygii Species 0.000 description 1
- 206010006187 Breast cancer Diseases 0.000 description 1
- 208000026310 Breast neoplasm Diseases 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 241001272567 Hominoidea Species 0.000 description 1
- 101001012157 Homo sapiens Receptor tyrosine-protein kinase erbB-2 Proteins 0.000 description 1
- 206010036790 Productive cough Diseases 0.000 description 1
- 102100030086 Receptor tyrosine-protein kinase erbB-2 Human genes 0.000 description 1
- 230000001464 adherent effect Effects 0.000 description 1
- 238000007664 blowing Methods 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 210000001072 colon Anatomy 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 230000018044 dehydration Effects 0.000 description 1
- 238000006297 dehydration reaction Methods 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 238000007598 dipping method Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000005338 frosted glass Substances 0.000 description 1
- 238000007429 general method Methods 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 238000012151 immunohistochemical method Methods 0.000 description 1
- 238000011532 immunohistochemical staining Methods 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 210000005228 liver tissue Anatomy 0.000 description 1
- 210000001165 lymph node Anatomy 0.000 description 1
- 210000000754 myometrium Anatomy 0.000 description 1
- 210000001672 ovary Anatomy 0.000 description 1
- TWNQGVIAIRXVLR-UHFFFAOYSA-N oxo(oxoalumanyloxy)alumane Chemical compound O=[Al]O[Al]=O TWNQGVIAIRXVLR-UHFFFAOYSA-N 0.000 description 1
- 210000002741 palatine tonsil Anatomy 0.000 description 1
- 230000007170 pathology Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 238000003672 processing method Methods 0.000 description 1
- 210000002307 prostate Anatomy 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000004080 punching Methods 0.000 description 1
- 108020003175 receptors Proteins 0.000 description 1
- 102000005962 receptors Human genes 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 238000012552 review Methods 0.000 description 1
- 239000012487 rinsing solution Substances 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000000377 silicon dioxide Substances 0.000 description 1
- 235000012239 silicon dioxide Nutrition 0.000 description 1
- 229910052814 silicon oxide Inorganic materials 0.000 description 1
- 210000003802 sputum Anatomy 0.000 description 1
- 208000024794 sputum Diseases 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 208000008732 thymoma Diseases 0.000 description 1
- 210000001685 thyroid gland Anatomy 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/2813—Producing thin layers of samples on a substrate, e.g. smearing, spinning-on
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/34—Microscope slides, e.g. mounting specimens on microscope slides
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/2813—Producing thin layers of samples on a substrate, e.g. smearing, spinning-on
- G01N2001/2833—Collecting samples on a sticky, tacky, adhesive surface
Landscapes
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Engineering & Computer Science (AREA)
- Optics & Photonics (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
Description
Claims (10)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202010318770.6A CN111504739A (en) | 2020-04-21 | 2020-04-21 | Immunohistochemical slide glass for detection |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202010318770.6A CN111504739A (en) | 2020-04-21 | 2020-04-21 | Immunohistochemical slide glass for detection |
Publications (1)
Publication Number | Publication Date |
---|---|
CN111504739A true CN111504739A (en) | 2020-08-07 |
Family
ID=71874447
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN202010318770.6A Pending CN111504739A (en) | 2020-04-21 | 2020-04-21 | Immunohistochemical slide glass for detection |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN111504739A (en) |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN113092218A (en) * | 2021-03-19 | 2021-07-09 | 北京龙迈达斯科技开发有限公司 | Teaching chip and preparation method thereof |
CN114002030A (en) * | 2021-10-15 | 2022-02-01 | 北京龙迈达斯科技开发有限公司 | Composite tissue chip and preparation method thereof |
CN115220210A (en) * | 2022-07-18 | 2022-10-21 | 上海大格生物科技有限公司 | Immunohistochemical quality control glass slide and quality control chip |
-
2020
- 2020-04-21 CN CN202010318770.6A patent/CN111504739A/en active Pending
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN113092218A (en) * | 2021-03-19 | 2021-07-09 | 北京龙迈达斯科技开发有限公司 | Teaching chip and preparation method thereof |
CN114002030A (en) * | 2021-10-15 | 2022-02-01 | 北京龙迈达斯科技开发有限公司 | Composite tissue chip and preparation method thereof |
CN115220210A (en) * | 2022-07-18 | 2022-10-21 | 上海大格生物科技有限公司 | Immunohistochemical quality control glass slide and quality control chip |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN111504739A (en) | Immunohistochemical slide glass for detection | |
US10031130B2 (en) | Device for high throughput detection of cervical disease | |
Kerstens et al. | AgarCyto: a novel cell-processing method for multiple molecular diagnostic analyses of the uterine cervix | |
CN108120762A (en) | Mass spectrum substrate and preparation method and purposes | |
Chen et al. | Excellent analytical and clinical performance of a dry self‐sampling device for human papillomavirus detection in an urban Chinese referral population | |
EP1015914A2 (en) | Microscope slide | |
CN102393318A (en) | Simple and quick preparation method of thin-layer liquid-based cytology cell smear | |
Sehgal et al. | Processing and reporting of cytology specimens from mediastinal lymph nodes collected using endobronchial ultrasound-guided transbronchial needle aspiration: a state-of-the-art review | |
CN108107216B (en) | Application of combined marker in preparation of pancreatic cancer prognosis kit and determination system and method thereof | |
Pai et al. | p16INK4A immunohistochemical staining may be helpful in distinguishing branchial cleft cysts from cystic squamous cell carcinomas originating in the oropharynx | |
Ercoli et al. | Building up a high-throughput screening platform to assess the heterogeneity of HER2 gene amplification in breast cancers | |
Kang et al. | p16 immunohistochemistry is less useful than MDM2 and CDK4 to distinguish dedifferentiated liposarcomas from other retroperitoneal mimics | |
Barkan | Enough is enough: adequacy of voided urine cytology | |
Tripathy et al. | Immunocytochemistry using liquid-based cytology: a tool in hormone receptor analysis of breast cancer | |
Søland et al. | High‐risk human papilloma virus was not detected in a Norwegian cohort of oral squamous cell carcinoma of the mobile tongue | |
Lv et al. | A novel solution configuration on liquid-based endometrial cytology | |
Brogi et al. | Ductal lavage in patients undergoing mastectomy for mammary carcinoma: a correlative study | |
CN210893865U (en) | Immunohistochemical slide glass for detection | |
Kumarasinghe | Australian system for reporting thyroid cytology | |
Gormley et al. | A simplified molecular method to detect high‐risk HPV using the Aptima HPV assay on head and neck FNA smears | |
Ao et al. | Fiber-Optic Array Scanning Technology (FAST) for detection and molecular characterization of circulating tumor cells | |
Minehart Miron | Preparation, culture, and analysis of amniotic fluid samples | |
Shi et al. | An update on immunohistochemistry in translational cancer research | |
Au et al. | Cytogenetic challenge assays for assessment of DNA repair capacities | |
CN113917160A (en) | Specificity method for detecting breast cancer circulating tumor cells by using HER2 antibody immunofluorescence method |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
TA01 | Transfer of patent application right | ||
TA01 | Transfer of patent application right |
Effective date of registration: 20220708 Address after: 200011 No. 639, manufacturing Bureau Road, Shanghai, Huangpu District Applicant after: SHANGHAI NINTH PEOPLE'S HOSPITAL SHANGHAI JIAOTONG University SCHOOL OF MEDICINE Address before: 200011 No. 639, manufacturing Bureau Road, Shanghai, Huangpu District Applicant before: SHANGHAI NINTH PEOPLE'S HOSPITAL SHANGHAI JIAOTONG University SCHOOL OF MEDICINE Applicant before: Xiamen medical association pathological diagnosis center Co.,Ltd. Applicant before: Shanghai yizhiben Technology Co.,Ltd. |
|
EE01 | Entry into force of recordation of patent licensing contract | ||
EE01 | Entry into force of recordation of patent licensing contract |
Application publication date: 20200807 Assignee: Jiangsu Kangke Investment Co.,Ltd. Assignor: SHANGHAI NINTH PEOPLE'S HOSPITAL SHANGHAI JIAOTONG University SCHOOL OF MEDICINE Contract record no.: X2023310000051 Denomination of invention: A glass slide for immunohistochemical detection License type: Common License Record date: 20230414 |