Stem cell separation device
Technical Field
The invention relates to the technical field of stem cell separation, in particular to a stem cell separation device.
Background
Stem cells have great significance in the medical field, and along with research of stem cells, the separation technology of stem cells is more and more paid attention to. The stem cell separation device can adopt a cell screen to separate, the efficiency of separation can be improved by pressurizing a sample in the separation process, the separation efficiency is improved by pressurizing an air bag in the prior art, but the air bag is pressurized to introduce external gas, so that the risk of sample pollution caused by introducing bacteria is still present even if a sterilizing filter is arranged for bacterial separation, meanwhile, the cell dispersion can not be promoted by forming reverse liquid flow through gas pressurization, and the separation efficiency can be influenced by the blockage generated by the cell screen.
Disclosure of Invention
Aiming at the prior art, the invention aims to provide the stem cell separation device which has the advantages of improving the cell separation efficiency, facilitating cleaning and disinfection, reducing the blockage of a cell screen and having high separation efficiency.
The technical scheme of the invention is realized as follows:
the utility model provides a stem cell separator, includes jar body, sleeve, cell screen cloth and pushes away the water piston, the sleeve both ends are equipped with the opening and are in through the support is fixed in the jar body, it is in to push away the water piston telescopic lower part motion, telescopic upper portion is equipped with the cell screen cloth, the sleeve with be equipped with first filter screen between the jar body, cell screen cloth below be equipped with the second filter screen in the sleeve, the second filter screen is equipped with first through-hole, first through-hole is equipped with the first one-way baffle of opening downwards, telescopic lateral wall is equipped with the second through-hole, the second through-hole is equipped with the second one-way baffle of outwards opening, be equipped with in pushing away the water piston with the metal piece, the jar is equipped with the magnet outward, the magnet with the metal piece looks mutual attraction, the magnet drive the metal piece is followed jar body up-and-down motion.
Further, the height position of the second through hole is smaller than that of the first through hole by 3-8 cm.
Further, the tank body is provided with a motor, a first deflector rod and a second deflector rod, an output shaft of the motor is provided with the first deflector rod and 2 second deflector rods, the length of the first deflector rod is larger than that of the second deflector rod, the tank body is sleeved with a slip ring, the slip ring is connected with the tank body through a spring, the magnet is fixed on the slip ring, the slip ring is provided with a protruding block, and the first deflector rod and the second deflector rod are used for pushing the protruding block to slide upwards.
Further, the water pushing piston comprises a piston head and a C-shaped ring, the metal block is arranged in the piston head, an annular groove is formed in the side face of the piston head, and the C-shaped ring is arranged in the annular groove.
Further, the C-shaped ring is made of a metal material.
Further, the cell screen is of a conical structure.
Further, the first filter screen is of a conical structure, and the first through holes are formed in the bottom of the cell screen.
Further, the tank body is provided with a cover, and the cover is connected with the tank body through threads.
Further, a clamping groove is formed in the inner wall of the tank body, and the support is clamped in the clamping groove.
The invention has the beneficial effects that: the cell soaking liquid forms an internal circulation in stem cell separation, and no additional injection of new liquid is needed. In the circulation, the liquid passes through the cell screen in an upward flowing direction and a downward flowing direction, the downward flowing promotes the separation of cells, the upward flowing promotes the cells to loosen, the blocking of the cell screen is reduced, and the stem cell separation is facilitated. In the circulation process, the first filter screen and the second filter screen both filter the cell soaking liquid, so that large-particle substances are prevented from returning to the upper part of the cell screen. In the downward reciprocating motion of the water pushing piston, the opening and closing of the first unidirectional baffle and the second unidirectional baffle can be automatically controlled, the operation of loosening cells can be carried out after the stem cells are filtered every two times, liquid flowing upwards each time is filtered, and liquid flowing back to the upper part of the cell screen is filtered. The water pushing piston can promote stem cells to separate when moving downwards, and pushes the cell soaking liquid back to the upper part of the cell screen. When the water pushing piston moves upwards, the cell soaking liquid in the sleeve can be discharged or loosened. The magnet drives the water pushing piston to move up and down outside the tank body, so that the tank body and parts in the tank body can be sterilized at high temperature, and the driving parts are prevented from polluting the environment in the tank body.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings required for the description of the embodiments will be briefly described below, it being obvious that the drawings in the following description are only preferred embodiments of the present invention, and that other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
FIG. 1 is a schematic cross-sectional view of a stem cell separation device according to the present invention;
FIG. 2 is a schematic diagram of the motor, first lever and second lever of the present invention;
in the figure, a tank body 1, a sleeve 2, a 3-cell screen mesh, a water pushing piston 4, a bracket 5, a first filter screen 6, a second filter screen 7, a first through hole 8, a first one-way baffle 9, a second through hole 10, a second one-way baffle 11, a metal block 12, a magnet 13, a motor 14, a first deflector rod 15, a second deflector rod 16, a slip ring 17, a spring 18, a C-shaped ring 19, a piston head 20, a ring groove 21, a cover 22, a clamping groove 23 and a lug 24.
Detailed Description
For a better understanding of the technical content of the present invention, specific examples are provided below and the present invention is further described with reference to the accompanying drawings.
Referring to fig. 1-2, a stem cell separation device, including a tank body 1, a sleeve 2, a cell screen 3 and a water pushing piston 4, the sleeve 2 both ends are equipped with the opening and fix through support 5 in the tank body 1, the water pushing piston 4 is in the lower part motion of sleeve 2, the upper portion of sleeve 2 is equipped with cell screen 3, sleeve 2 with be equipped with first filter screen 6 between the tank body 1, be equipped with second filter screen 7 in the sleeve 2 of cell screen 3 below, second filter screen 7 is equipped with first through-hole 8, first through-hole 8 is equipped with the first one-way baffle 9 of opening downwards, the lateral wall of sleeve 2 is equipped with second through-hole 10, second through-hole 10 is equipped with the second one-way baffle 11 of opening outwards, be equipped with in the water pushing piston 4 with metal piece 12, the external magnet 13 that is equipped with of tank body 1, magnet 13 with metal piece 12 attracts each other, magnet 13 drive metal piece 12 is followed tank body 1 up-down motion.
The cell sample liquid to be separated is poured into the sleeve 2, a cell screen 3 is arranged at the upper part in the sleeve 2, and the stem cells are separated by the cell screen 3. The upper end and the lower end of the sleeve 2 are provided with openings, the sleeve 2 is arranged in the tank body 1, and cell soaking liquid can flow in from the upper end of the sleeve 2 and flow out from the lower end. The lower part of the sleeve 2 is provided with a water pushing piston 4, and when the water pushing piston 4 moves downwards, the cell soaking liquid is pumped into the sleeve 2. The lateral wall of sleeve 2 is equipped with second through-hole 10, and second through-hole 10 is equipped with the second one-way baffle 11 of outwards opening, and optionally, second one-way baffle 11 has elasticity, and one side and sleeve 2 are connected, cell screen 3 below be equipped with second filter screen 7 in the sleeve 2, second filter screen 7 is equipped with first through-hole 8, first through-hole 8 is equipped with the first one-way baffle 9 of opening downwards, and optionally, first one-way baffle 9 has elasticity, and one side and second filter screen 7 are connected. When the water pushing piston 4 moves downward under the second through hole 10: the second one-way baffle 11 covers the second through hole 10 under the action of water flow, so that the second through hole 10 is sealed, the cell soaking liquid cannot flow into the sleeve 2 from the second through hole 10, meanwhile, the first one-way baffle 9 moves downwards, the first through hole 8 is opened, so that the cell soaking liquid flows from the upper part to the lower part of the sleeve 2, the separation of stem cells by the cell screen 3 is promoted, the cell soaking liquid in the sleeve 2 flows into a gap between the sleeve 2 and the tank 1 from the bottom of the sleeve 2, and overflows upwards through the first filter screen 6 to flow back to the upper part of the sleeve 2, and because the pore diameters of the first filter screen 6 and the second filter screen 7 are smaller than those of the cell screen 3 through the filtering of the first filter screen 6, large-particle substances and cells cannot return to the top of the sleeve 2. When the water pushing piston 4 moves in the lower direction of the second through hole 10, the first one-way baffle 9 moves upward to block the first through hole 8, the first through hole 8 is closed, and the second one-way baffle 11 opens outward, so that the cell soaking liquid flows out of the sleeve 2 from the second through hole 10. When the water pushing piston 4 moves upwards to the second through hole 10 and then continues to move upwards, the second through hole 10 is blocked by the water pushing piston 4 or is positioned below the water pushing piston 4, the first through hole 8 and the second through hole 10 are both closed, the cell soaking liquid is pushed to flow upwards through the second filter screen 7, large particulate matters and cells are blocked by the grids under the filtering effect of the filter screen, the cell soaking liquid flows upwards to impact the cell screen 3, cells above the cell screen 3 are loosened, and the next cell screen 3 is convenient for separating stem cells. When the water pushing piston 4 reaches the top limit position and moves downwards, the first one-way baffle 9 moves downwards to open the first through hole 8 and the cell soaking liquid flows downwards to promote the cell screen 3 to separate cells. Be equipped with in the push away water piston 4 with the metal piece 12, the jar body 1 is equipped with magnet 13 outward, magnet 13 with metal piece 12 inter attraction, magnet 13 drive the metal piece 12 is followed jar body 1 up-and-down motion, magnet 13 locate jar body 1 outside, rethread magnetic field drive push away water piston 4 up-and-down motion, avoid actuating mechanism to contact jar body 1 inside, jar body 1, sleeve 2, push away parts such as water piston 4 all can carry out the high temperature sterilization, ensure that stem cell separation process is not polluted, and the device is clean and disinfection easily. The magnet 13 reciprocates 2 times below the second through hole 10 and then reciprocates 1 time above the second through hole 10.
Preferably, the height position of the second through hole 10 is smaller than the height position of the first through hole 8 by 3-8 cm. The water pushing piston 4 has a certain moving space above the second through hole 10, so that the water pushing is convenient to loosen cells.
Optionally, the motor 14, the first deflector rod 15 and the second deflector rod 16 are arranged outside the tank body 1, one first deflector rod 15 and 2 second deflector rods 16 are arranged on an output shaft of the motor 14, the length of the first deflector rod 15 is greater than that of the second deflector rod 16, the tank body 1 is sleeved with the sliding ring 17, the sliding ring 17 is connected with the tank body 1 through a spring 18, the magnet 13 is fixed on the sliding ring 17, the sliding ring 17 is provided with a protruding block 24, and the first deflector rod 15 and the second deflector rod 16 are used for pushing the protruding block 24 to slide upwards. The first deflector rod 15 and the second deflector rod 16 are perpendicular to the output shaft of the motor 14, and when the motor 14 rotates, the first deflector rod 15 and the second deflector rod 16 rotate, so that the lug 24 is pushed to move upwards, and the slip ring 17 is driven to move upwards. Since the length of the first shift lever 15 is longer than the length of the second shift lever 16, the distance that the first shift lever 15 pushes the slip ring 17 upward is longer than the distance that the second shift lever 16 pushes the slip ring 17 upward, thereby ensuring that the magnet 13 reciprocates 2 times below the second through hole 10 and then reciprocates 1 time above the second through hole 10. The tank body 1 is sleeved with the slip ring 17, the slip ring 17 can move up and down, the magnet 13 moves up and down along with the slip ring 17, the magnet 13 is prevented from deviating, and the components such as the tank body 1 can be sterilized after the magnet 13 is taken down. Preferably, the motor 14 outside the tank 1 is fixed on the base, and the tank 1 is clamped with the base. After the projection 17 loses the pushing force, the slip ring 17 moves downward to return under the pulling force of the spring 18.
Optionally, the water pushing piston 4 includes a piston head 20 and a plurality of C-shaped rings 19, the metal block 12 is disposed in the piston head 20, a ring groove 21 is disposed on a side surface of the piston head 20, and the C-shaped rings 19 are disposed in the ring groove 21. The C-shaped ring 19 is made of elastic materials, and annular openings are staggered, so that the sealing performance of the water pushing piston 4 is improved, and the water pushing capability is provided.
Preferably, the C-ring 19 is made of a metal material. Is made of a magnetizable metal material, is convenient to be close to the sleeve 2 under the action of the magnet 13, and improves the sealing effect.
Specifically, the cell screen 3 has a conical structure. The surface area of the cell screen 3 is increased, and the cell separation effect is improved.
Specifically, the first filter screen 6 has a conical structure, and the first through hole 8 is formed in the bottom of the cell screen 3. The area of the filter screen is increased, and the filtering effect is improved.
Specifically, the can body 1 is provided with a cover 22, and the cover 22 and the can body 1 are connected through threads. The tank 1 is covered by a cover 22 to avoid overflow of the cell soaking liquid during the separation process.
Optionally, a clamping groove 23 is formed in the inner wall of the tank body 1, and the bracket 5 is clamped in the clamping groove 23. The bracket 5 is clamped in the pipe body, and can be detached during cleaning and disinfection, so that the operation is convenient.
The foregoing description of the preferred embodiments of the invention is not intended to be limiting, but rather is intended to cover all modifications, equivalents, alternatives, and improvements that fall within the spirit and scope of the invention.