CN111492894A - Method for cultivating ganoderma lucidum spore powder - Google Patents
Method for cultivating ganoderma lucidum spore powder Download PDFInfo
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- CN111492894A CN111492894A CN202010345370.4A CN202010345370A CN111492894A CN 111492894 A CN111492894 A CN 111492894A CN 202010345370 A CN202010345370 A CN 202010345370A CN 111492894 A CN111492894 A CN 111492894A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/60—Cultivation rooms; Equipment therefor
- A01G18/69—Arrangements for managing the environment, e.g. sprinklers
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N3/00—Spore forming or isolating processes
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Abstract
The invention provides a method for cultivating ganoderma lucidum spore powder, which comprises the following steps: s1, selecting ganoderma lucidum strains with good spore quality, strong disease resistance and high yield, S1, selecting a mountain area of a subtropical climate area with the average temperature maintained at 20-30 ℃ as a breeding area, manufacturing a cultivation shed, S3, manufacturing a basswood culture medium in the cultivation shed, S4, transplanting the selected ganoderma lucidum strains to the basswood culture medium, S5, controlling the ganoderma lucidum output quantity of each strain rod when ganoderma lucidum is output, and controlling the growth of one ganoderma lucidum by each strain rod when the diameter of the strain rod of the ganoderma lucidum is 10-15cm and the length of the strain rod is 15-20 cm; when the diameter of the ganoderma lucidum stick is 10-15cm and the length of the ganoderma lucidum stick is 25-30cm, two ganoderma lucidum sticks are controlled to grow on each stick. The method for cultivating the ganoderma lucidum spore powder selects the excellent ganoderma lucidum variety, the proper culture medium, the proper planting place and the elaborate ganoderma lucidum management, and can greatly improve the yield and the quality of the ganoderma lucidum spore powder.
Description
Technical Field
The invention relates to the technical field of edible and medicinal fungus cultivation, in particular to a method for cultivating ganoderma lucidum spore powder.
Background
Ganoderma, also known as Rumex, Ganoderma lucidum, Mesona chinensis Benth, Yao grass, caulis et folium Gaultheriae Yunnanensis, LINZHONGLING, JUNLINGZHI, WANNIANZHU, LINGCAO, CHIZHI, DANZHI, QINGZHEN, etc., is a fruiting body of Ganoderma lucidum belonging to Polyporaceae. The shape of the mushroom cap is umbrella-shaped, kidney-shaped, semicircular or nearly circular. Ganoderma lucidum is recorded in Ben Cao gang mu compiled by Li Shi Zhen of Ming Dynasty medical scientists.
Ganoderma spore is the seeds of Ganoderma lucidum which is the tiny egg-shaped germ cells ejected from Ganoderma lucidum fold during the growth and maturation period. Each ganoderma spore is only 4-6 microns, is a living organism, has a double-wall structure, is surrounded by hard chitin cellulose, and is difficult to be fully absorbed by a human body. After the wall is broken, the medicine is more suitable for direct absorption of intestines and stomach of human body. The Ganoderma spore powder has all genetic materials of Ganoderma and health promotion effect. The medicinal value of the ganoderma lucidum spore is increasingly valued, and researches show that the ganoderma lucidum spore has the effects of enhancing the immunity of organisms, inhibiting tumors, protecting liver injury and protecting radiation.
The ganoderma lucidum spore powder cultivation and collection is after artificial cultivation of ganoderma lucidum is developed, and has a history of more than 30 years to date. In the last 80 th century, ganoderma lucidum is replaced by indoor bottle cultivation, and spore powder is cultivated and collected by a bagging method, along with the development of imitating wild cultivation of basswood ganoderma lucidum, the growth environment of ganoderma lucidum is fundamentally changed, an original set of indoor bottle cultivation method is not suitable, however, the yield of cultivated ganoderma lucidum spore powder is low due to the fact that the existing field cultivation technology for ganoderma lucidum spore powder is immature, and how to cultivate high-quality and high-yield field basswood ganoderma lucidum spore powder becomes an important and urgent problem for spore powder development.
Disclosure of Invention
Aiming at the defects of the prior art, the invention provides a method for cultivating ganoderma lucidum spore powder, which solves the problem of low yield of ganoderma lucidum spore powder cultivated in the field.
In order to achieve the purpose, the invention is realized by the following technical scheme: a method for cultivating ganoderma lucidum spore powder is characterized in that: the method comprises the following steps:
s1, selecting a ganoderma lucidum strain with good spore quality, strong disease resistance and high yield;
s2, selecting a mountain area of a subtropical climate area with the average temperature maintained at 20-30 ℃ as a breeding ground, and manufacturing a cultivation shed;
s3, preparing a basswood culture medium in the cultivation shed;
s4, transplanting the selected ganoderma lucidum strains to a basswood culture medium;
s5, controlling the ganoderma lucidum output quantity of each fungus stick when ganoderma lucidum generates, and controlling each fungus stick to grow one ganoderma lucidum when the diameter of a stick body of the ganoderma lucidum stick is 10-15cm and the length of the stick body of the ganoderma lucidum stick is 15-20 cm; when the diameter of the ganoderma lucidum stick body is 10-15cm and the length is 25-30cm, controlling two ganoderma lucidum growing on each fungus stick;
s6, adjusting the internal environment of the cultivation shed when the lucid ganoderma goes out, adjusting the relative humidity of air in the cultivation shed to 75% -85%, and adjusting the concentration of carbon dioxide in the cultivation shed to 0.1%;
and S7, sleeving the lucid ganoderma when the lucid ganoderma begins to have white edges, and collecting lucid ganoderma spore powder 10 days after sleeving.
Preferably, the preparation process of the basswood culture medium is as follows:
(1) selecting seeds of a fagaceae tree as a raw material for preparing basswood;
(2) cutting the selected basswood raw material into wood sections with the length of 15-20cm, and ensuring that two end faces of the wood sections are flush;
(3) preparing nutrient solution for soaking basswood;
(4) placing the cut basswood into a nutrient solution pool, soaking for 5-8 days, fishing out, standing for 1 day, bagging and sterilizing;
(5) and (4) fixedly placing the drained basswood culture medium in a cultivation shed at equal intervals, and finishing the preparation of the basswood culture medium.
Preferably, the sapling of the family Fagaceae selected as the raw material for preparing the basswood comprises one of chestnut, Conus, cyclobalanopsis, Coco and Quercus.
Preferably, the nutrient solution comprises water, a glucose solution, a zinc sulfate solution and an additive, wherein the water, the glucose solution and the zinc sulfate solution are mixed according to the proportion of 500 parts of water, 100 parts of the glucose solution, 10 parts of the zinc sulfate solution and 20 parts of the additive.
Preferably, the additive is prepared by mixing medicinal juices of pinellia ternate, caulis spatholobi, eucommia ulmoides, safflower, Chinese yam and the like, wherein the ratio of the pinellia ternate to the caulis spatholobi to the eucommia ulmoides, the safflower and the Chinese yam is 1: 1: 2: 2: 1.
preferably, the preparation method of the nutrient solution comprises the following steps: pouring the prepared water into the inner end of the water pool, then pouring the prepared glucose solution, zinc sulfate solution and additive into the water pool in sequence, and then fully stirring and mixing.
Preferably, when the white edge of the ganoderma lucidum begins to appear and the ganoderma lucidum is sleeved, a layer of plastic film is laid on the bottom surface of the ganoderma lucidum.
Compared with the prior art, the invention has the following beneficial effects:
(1) the culture method provided by the invention selects high-quality ganoderma lucidum strains for cultivation, ensures that the cultured ganoderma lucidum has good genes, fundamentally increases the yield of the ganoderma lucidum, and simultaneously selects subtropical environments suitable for the growth of the ganoderma lucidum for cultivation, and ensures that the ganoderma lucidum has good growth environments.
(2) The culture method provided by the invention selects the fagaceae tree species to prepare the basswood culture medium, and simultaneously prepares the nutrient solution to soak the basswood, so that the ganoderma lucidum has enough nutrition guarantee during growth, and high-quality variation can be generated during the growth process, so that the grown ganoderma lucidum spore powder has the effects of enriching zinc and polysaccharide, and the ganoderma lucidum spore powder also has the effects of the additive, and the efficacy value of the ganoderma lucidum spore powder is greatly improved.
(3) According to the cultivation method provided by the invention, when the lucid ganoderma produces the lucid ganoderma, the lucid ganoderma is properly produced and managed according to the growth characteristics of the lucid ganoderma, and the internal environment of the cultivation shed is adjusted to improve the output quality and quantity of lucid ganoderma spore powder.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The first embodiment is as follows:
a method for cultivating ganoderma lucidum spore powder comprises the following steps:
s1, selecting 'Longzhi No. I' as a lucid ganoderma strain for culture;
s1, selecting a mountain area of a Longquan of Zhejiang as a cultivation land and manufacturing a cultivation shed;
s3, preparing a basswood culture medium in a cultivation shed, wherein the culture medium selects cyclobalanopsis glauca seeds as basswood, and the nutrient solution for the culture medium comprises 10kg of water, 2kg of glucose solution, 200g of zinc sulfate solution and 400g of additive;
s4, transplanting the selected ganoderma lucidum strains to a basswood culture medium;
s5, controlling the ganoderma lucidum output quantity of each fungus stick when ganoderma lucidum generates, and controlling each fungus stick to grow one ganoderma lucidum when the diameter of a stick body of the ganoderma lucidum stick is 10-15cm and the length of the stick body of the ganoderma lucidum stick is 15-20 cm; when the diameter of the ganoderma lucidum stick body is 10-15cm and the length is 25-30cm, controlling two ganoderma lucidum growing on each fungus stick;
s6, adjusting the internal environment of the cultivation shed when the lucid ganoderma goes out, adjusting the relative humidity of air in the cultivation shed to 75%, and adjusting the concentration of carbon dioxide in the cultivation shed to 0.1%;
and S7, sleeving the lucid ganoderma when the lucid ganoderma begins to have white edges, and collecting lucid ganoderma spore powder 10 days after sleeving.
Example two:
s1, selecting Shanghan ganoderma lucidum I as a ganoderma lucidum strain for culture;
s1, selecting a mountain area of a Longquan of Zhejiang as a cultivation land and manufacturing a cultivation shed;
s3, preparing a basswood culture medium in a cultivation shed, wherein white birch seeds are selected as the basswood, and meanwhile, the nutrient solution for the culture medium comprises 10kg of water, 2kg of glucose solution, 200g of zinc sulfate solution and 400g of additive;
s4, transplanting the selected ganoderma lucidum strains to a basswood culture medium;
s5, controlling the ganoderma lucidum output quantity of each fungus stick when ganoderma lucidum generates, and controlling each fungus stick to grow one ganoderma lucidum when the diameter of a stick body of the ganoderma lucidum stick is 10-15cm and the length of the stick body of the ganoderma lucidum stick is 15-20 cm; when the diameter of the ganoderma lucidum stick body is 10-15cm and the length is 25-30cm, controlling two ganoderma lucidum growing on each fungus stick;
s6, adjusting the internal environment of the cultivation shed when the lucid ganoderma goes out, adjusting the relative humidity of air in the cultivation shed to 80%, and adjusting the concentration of carbon dioxide in the cultivation shed to 0.1%;
and S7, sleeving the lucid ganoderma when the lucid ganoderma begins to have white edges, and collecting lucid ganoderma spore powder 10 days after sleeving.
The invention is more effectively illustrated by the following experimental results obtained by a plurality of experiments:
the invention firstly inspects the influence of ganoderma lucidum variety, planting place, tree species and whether ganoderma lucidum production management is carried out on ganoderma lucidum spore powder yield, and the results are shown in the following table 1 by taking 1 ton of culture medium wood as a unit:
table 1:
as can be seen from the comparison of the data in Table 1, the selection of good ganoderma lucidum varieties, suitable planting places and Fagaceae tree varieties can greatly improve the yield of ganoderma lucidum spore powder.
The invention then examines whether the nutrient solution is used to influence the contents of polysaccharide and zinc element in the ganoderma lucidum spore powder, and the results are shown in the following table 2:
table 2:
according to the comparison results obtained in table 2, it can be known that the addition of the special nutrient solution provided by the invention can greatly improve the polysaccharide content and the zinc element content in the produced ganoderma lucidum spore powder, thereby greatly increasing the nutritional value of the ganoderma lucidum spore powder.
The invention also investigates the influence of different ganoderma lucidum output management on the yield of ganoderma lucidum cheongzi powder, and the results are shown in the following table 3:
table 3:
as can be seen from the data in Table 3, when the glossy ganoderma starts to produce glossy ganoderma, the quantity of the produced glossy ganoderma is adjusted according to the growth condition of the glossy ganoderma on each fungus stick, which is beneficial to the yield of glossy ganoderma spore powder, when the length of the glossy ganoderma is shorter, the nutrition taken by a single glossy ganoderma is less, and the quantity of the produced glossy ganoderma on the fungus stick needs to be reduced; on the contrary, when the length of the ganoderma lucidum is longer, the nutrition taken by a single ganoderma lucidum is more, and the number of the ganoderma lucidum on the fungus stick can be properly increased so as to increase the yield of ganoderma lucidum spore powder.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (7)
1. A method for cultivating ganoderma lucidum spore powder is characterized in that: the method comprises the following steps:
s1, selecting a ganoderma lucidum strain with good spore quality, strong disease resistance and high yield;
s2, selecting a mountain area of a subtropical climate area with the average temperature maintained at 20-30 ℃ as a breeding ground, and manufacturing a cultivation shed;
s3, preparing a basswood culture medium in the cultivation shed;
s4, transplanting the selected ganoderma lucidum strains to a basswood culture medium;
s5, controlling the ganoderma lucidum output quantity of each fungus stick when ganoderma lucidum generates, and controlling each fungus stick to grow one ganoderma lucidum when the diameter of a stick body of the ganoderma lucidum stick is 10-15cm and the length of the stick body of the ganoderma lucidum stick is 15-20 cm; when the diameter of the ganoderma lucidum stick body is 10-15cm and the length is 25-30cm, controlling two ganoderma lucidum growing on each fungus stick;
s6, adjusting the internal environment of the cultivation shed when the lucid ganoderma goes out, adjusting the relative humidity of air in the cultivation shed to 75% -85%, and adjusting the concentration of carbon dioxide in the cultivation shed to 0.1%;
and S7, sleeving the lucid ganoderma when the lucid ganoderma begins to have white edges, and collecting lucid ganoderma spore powder 10 days after sleeving.
2. The method for cultivating ganoderma lucidum spore powder according to claim 1, wherein the method comprises the following steps: the preparation process of the basswood culture medium is as follows:
(1) selecting seeds of a fagaceae tree as a raw material for preparing basswood;
(2) cutting the selected basswood raw material into wood sections with the length of 15-20cm, and ensuring that two end faces of the wood sections are flush;
(3) preparing nutrient solution for soaking basswood;
(4) placing the cut basswood into a nutrient solution pool, soaking for 5-8 days, then taking out and placing for 1 day, and then carrying out bagging sterilization treatment;
(5) and (4) fixedly placing the drained basswood culture medium in a cultivation shed at equal intervals, and finishing the preparation of the basswood culture medium.
3. The method for cultivating ganoderma lucidum spore powder according to claim 2, wherein the method comprises the following steps: the seed of Fagaceae tree is selected from one of Castanea, Conium, cyclobalanopsis, Coca and Quercus.
4. The method for cultivating ganoderma lucidum spore powder according to claim 2, wherein the method comprises the following steps: the nutrient solution comprises water, a glucose solution, a zinc sulfate solution and an additive, wherein the water, the glucose solution and the zinc sulfate solution are mixed according to the proportion of 500 parts of water, 100 parts of the glucose solution, 10 parts of the zinc sulfate solution and 20 parts of the additive.
5. The method for cultivating ganoderma lucidum spore powder according to claim 2, wherein the method comprises the following steps: the additive is prepared by mixing the medicinal juices of pinellia ternate, caulis spatholobi, eucommia ulmoides, safflower and Chinese yam, wherein the composition ratio of the pinellia ternate, the caulis spatholobi, the eucommia ulmoides, the safflower and the Chinese yam is 1: 1: 2: 2: 1.
6. the method for cultivating ganoderma lucidum spore powder according to claim 1, wherein the method comprises the following steps: the preparation method of the nutrient solution comprises the following steps: pouring the prepared water into a water pool, then pouring the prepared glucose solution, zinc sulfate solution and additive into the water pool in sequence, and then fully stirring and mixing.
7. The method for cultivating ganoderma lucidum spore powder according to claim 1, wherein the method comprises the following steps: when the white edge of the lucid ganoderma begins to appear and the lucid ganoderma is sleeved, a layer of plastic film is laid on the bottom surface of the lucid ganoderma.
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2020
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| CN110122172A (en) * | 2019-06-05 | 2019-08-16 | 宁德市益智源农业开发有限公司 | A kind of needle mushroom mushroom bran compost and its cultural method for hayashishita ganoderma lucidum |
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