CN111467255A

CN111467255A - Preparation method and device of VC collagen anti-wrinkle essence capable of preventing skin aging

Info

Publication number: CN111467255A
Application number: CN202010316182.9A
Authority: CN
Inventors: 刘佳沛; 张勇
Original assignee: Jiangsu Gengmei Technology Co ltd
Current assignee: Jiangsu Gengmei Technology Co ltd
Priority date: 2020-04-21
Filing date: 2020-04-21
Publication date: 2020-07-31
Anticipated expiration: 2040-04-21
Also published as: CN111467255B

Abstract

The embodiment of the invention discloses a preparation device of VC collagen anti-wrinkle essence for preventing skin aging, which comprises a centrifugal seat cylinder and a preparation cylinder sleeved in the centrifugal seat cylinder, wherein the top end of the preparation cylinder is connected with a sealing cover, the inner wall of the preparation cylinder is hermetically connected with a separation frame with a V-shaped cross section, the outer surface of the bottom of the separation frame is provided with a separation cover with a U-shaped cross section, the device can realize the quantitative discharge of filtrate through a quantitative water guide sleeve, the discharge of the filtrate is realized by means of gravity in the discharge process without manual extraction, so that the hands of an operator are released, the operation error probability is reduced, and then the precipitate can not flow away along with supernatant liquid by filtering through a filtering ring cover, and the raw materials can be stirred and smashed during extraction, so that the extraction rate of collagen is higher, and the device structure is compact, during the extraction and filtration operation, the solution can not contact the outside for a long time, so that the pollution probability is reduced.

Description

Preparation method and device of VC collagen anti-wrinkle essence capable of preventing skin aging

Technical Field

The embodiment of the invention relates to the field of collagen preparation, and particularly relates to a preparation method and device of VC collagen anti-wrinkle essence capable of preventing skin aging.

Background

Collagen is a family of proteins, and at least 30 genes encoding collagen chains have been found, and more than 16 kinds of collagen can be formed, and according to their structures, they can be classified into fibrous collagen, basement membrane collagen, microfibril collagen, anchored collagen, hexagonal network collagen, non-fibrous collagen, transmembrane collagen, and the like. Collagen can be classified into interstitial collagen, basement membrane collagen and extracellular and peripheral collagen according to their distribution and functional characteristics in the body. The interstitial collagen molecules account for the vast majority of collagen in the whole body, and comprise collagen molecules I, II and III, wherein the collagen I is mainly distributed in tissues such as skin, tendon and the like, is protein with the highest content of aquatic product processing wastes (skin, bone and scale), accounts for about 80-90% of the total collagen content, and is most widely applied in medicine.

Because collagen is an extracellular matrix component, exists in an insoluble macromolecular structure in vivo, and is combined with proteoglycan, glycoprotein and the like, different devices are needed to be used for material selection, pretreatment, acid-base enzyme-saline extraction, separation and purification of different types of collagen and other operations during the preparation of the collagen.

However, the common preparation devices in the current market generally face that after centrifugal operation is performed on material filtrate, supernatant and sediments exist in a test tube, then a suction device is used for sucking out the supernatant, and the purpose of sucking out the supernatant can be realized only by auxiliary equipment (such as a suction tube) during operation, but the limitation of the equipment on an operator during operation is large, once the operation is failed (such as the suction force is large), a large amount of sediments are easily sucked out again, so that the working time is greatly prolonged; and the purification process needs more steps, such as multiple operations of centrifugal separation and filtration, which makes the solution contact with the external environment for a longer time, i.e. the solution has a higher probability of being polluted.

Disclosure of Invention

Therefore, the embodiment of the invention provides a preparation method and a device of VC collagen anti-wrinkle essence for preventing skin aging, which aim to solve the problems that in the prior art, when centrifugal operation is carried out on material filtrate, supernatant and sediment exist in a test tube, then a suction device is used for sucking out the supernatant, the purpose of sucking out the supernatant can be realized only by auxiliary equipment during operation, but the limitation on operators is large, and once the operation is in error, a large amount of sediment is easily sucked out again; and the steps required in the purification process are more, if the operations of centrifugal separation and filtration are required to be carried out for many times, the solution can contact the external environment for a longer time, and the probability of solution pollution is higher.

In order to achieve the above object, an embodiment of the present invention provides the following:

a preparation device of VC collagen anti-wrinkle essence capable of preventing skin aging comprises a centrifugal seat barrel and a preparation barrel sleeved in the centrifugal seat barrel, wherein the top end of the preparation barrel is connected with a blocking cover, the inner wall of the preparation barrel is hermetically connected with a separation frame with a V-shaped cross section, the outer surface of the bottom of the separation frame is provided with a separation cover with a U-shaped cross section, the other end of the separation cover is hermetically connected with the inner wall of the bottom of the preparation barrel, the central position of the inner wall of the bottom of the separation frame is provided with a discharging mechanism, and the discharging mechanism is connected with a liquid guiding and fetching mechanism;

the row material mechanism includes that the tip is installed at the row's of separating frame bottom inner wall material section of thick bamboo, the other end of arranging the material section of thick bamboo with the top inner wall connection of closing off the cover, just the lateral wall of arranging the material section of thick bamboo sets up the transversal relief hole of personally submitting the splayed structure the inside wall of arranging the material section of thick bamboo has the ration to lead the water jacket through threaded connection the water jacket is led on the ration inner wall to install the connection pad that the surface was seted up there is the small opening the central point of connection pad puts the department and installs the tensile post that is used for the pulling ration to lead the water jacket, it runs through the closing off the cover to draw post one end, just the other end of.

As a preferable scheme of the present invention, the quantitative water guide sleeve comprises a main sleeve connected with an inner wall of the discharge cylinder, one end of the main sleeve close to the blocking cover is provided with a guide ring having a splayed cross section, and the other end of the main sleeve is provided with a polymerization cover for polymerizing the filtrate and making the filtrate flow down along the stretching column.

As a preferable scheme of the present invention, a filter ring cover is movably connected to an inner wall of the guide ring, the filter ring cover is composed of a support ring connected to the inner wall of the guide ring, a filter ring is installed at one end of the support ring away from the connection disc, and the thickness value of the filter ring is set in a linearly decreasing trend from a central position to the outside.

As a preferable scheme of the invention, a rotary sleeve is sleeved on the outer side of the discharge cylinder, a through hole is formed in the position of the side wall of the rotary sleeve corresponding to the discharge hole, a breaking sheet group is mounted on the side wall of the rotary sleeve, and a gravity rod is mounted at one end of the breaking sheet group, which is far away from the rotary sleeve.

As a preferable scheme of the invention, the breaking sheet group is composed of a plurality of breaking sheets, and the length values of the plurality of breaking sheets are set in a linearly decreasing trend from one end close to the blocking cover to one end far away from the blocking cover.

As a preferable scheme of the present invention, the liquid guiding and fetching mechanism includes a liquid guiding column tube located in the separating cover and hermetically connected to one end of the stretching column, and a liquid throwing device installed at the other end of the stretching column, a fetching device is installed on a side wall of the liquid guiding column tube, a liquid distributing block with a triangular cross section is installed in the liquid guiding column tube, two liquid distributing tubes with L-shaped structures are hermetically installed at one end of the liquid guiding column tube far away from the stretching column, the fetching device includes a leaking cover installed on a side wall of the liquid guiding column tube and having a L-shaped structure, and an isolating ring block is installed at one end of the leaking cover close to the stretching column.

In a preferred embodiment of the present invention, the liquid feeder includes a three-terminal column tube having one end hermetically connected to the tension column, a through guide column hermetically mounted to the other end of the three-terminal column tube, and an insufflation balloon hermetically connected to the three-terminal column tube and configured to insufflate liquid in the through guide column into the tension column.

As a preferred scheme of the invention, the guiding column comprises a liquid storage pipe which is hermetically connected with a three-terminal column pipe and is in a U-shaped structure, an arc-shaped cavity is arranged in the liquid storage pipe, a liquid guide cavity which is communicated with the three-terminal column pipe and has an isosceles trapezoid-shaped cross section is arranged at one end of the arc-shaped cavity, an adjusting cavity is arranged at the other end of the arc-shaped cavity, a glue pushing block is connected in the adjusting cavity, and a threaded column which penetrates through the adjusting cavity to the outer side is arranged on the surface of one side, far away from the arc-shaped cavity, of the glue pushing block.

A preparation method of VC collagen anti-wrinkle essence capable of preventing skin aging comprises the following steps:

s100, pretreating fish scales, pouring the pretreated fish scales into a preparation cylinder, adding 0.5 mol/L acetic acid at the temperature of 4 ℃, soaking for 24 hours, putting the preparation cylinder into a centrifugal seat cylinder to perform high-speed centrifugal operation, so that the extraction efficiency of farther protein is higher, filtering the extracted collagen raw liquid into a leakage cover through a discharger, storing filter residues in the leakage cover, and keeping filtrate in a separation cover;

s200, then putting the preparation cylinder into a centrifugal seat cylinder to perform centrifugal operation again, then adding 0.5 mol/L acetic acid and 0.5% pepsin into a separation cover at the temperature of 4 ℃, soaking for 24 hours at the same time, performing extraction operation again, and then performing centrifugal operation on the solution;

s300, standing to enable the solution in the separation cover to be divided into an upper layer and a lower layer, taking the supernatant as crude collagen liquid, and performing electrophoresis on the crude collagen liquid to obtain a finished product.

As a preferred embodiment of the present invention, in step S100, the preparation cylinder is placed in a centrifuge bowl to perform high-speed centrifugation operation, so that the efficiency of distant protein extraction is higher, and the specific operation steps are as follows:

s101, the preparation cylinder is driven to rotate by the centrifugal seat cylinder, and then the crushing sheet group in the preparation cylinder is driven to rotate, so that the fish scales are stirred and crushed, and the soaked fish scales are better and the collagen extraction degree is higher;

s102, rotating the quantitative water guide sleeve to enable the soak solution to flow into the quantitative water guide sleeve through the discharge hole, enabling the soak solution in the quantitative water guide sleeve to flow into the leakage cover to be filtered, and filtering filter residues.

The embodiment of the invention has the following advantages:

the equipment can realize the quantitative discharge of the filtrate through the quantitative water guide sleeve, is simpler in specific operation, can realize the discharge of the filtrate by directly using gravity in the discharge process without manual extraction, thereby releasing the hands of an operator, simultaneously reducing the probability of misoperation, and then filtering through the filtering ring cover, so that precipitates cannot easily flow away along with supernatant, and the raw materials can be stirred and smashed during extraction, so that the extraction efficiency of collagen is higher, meanwhile, the equipment structure is compact, when the operator performs extraction and filtration operation, other equipment is not needed, namely, the solution cannot contact the outside for a long time, so that the pollution probability is reduced;

and this equipment still can realize the ration input of liquid through leading the post, when adding and drawing the auxiliary additive promptly, accessible extrusion gasbag blows in the filtrating of separating the cover with the additive slowly, and its easy operation is convenient, and the control input that can relax and reduced liquid input in-process misoperation's probability (like liquid input excessive probability), and still can carry out filtration operation once more to the filtrating of inflow in the separating the cover through leaking the thing cover, later can also directly take out the solid filter residue for subsequent operation is simpler swift, has fully reduced artifical labour.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below. It should be apparent that the drawings in the following description are merely exemplary, and that other embodiments can be derived from the drawings provided by those of ordinary skill in the art without inventive effort.

The structures, ratios, sizes, and the like shown in the present specification are only used for matching with the contents disclosed in the specification, so as to be understood and read by those skilled in the art, and are not used to limit the conditions that the present invention can be implemented, so that the present invention has no technical significance, and any structural modifications, changes in the ratio relationship, or adjustments of the sizes, without affecting the effects and the achievable by the present invention, should still fall within the range that the technical contents disclosed in the present invention can cover.

FIG. 1 is a schematic overall structure diagram of an embodiment of the present invention;

FIG. 2 is a front partial cross-sectional view of a guide post according to an embodiment of the present invention;

FIG. 3 is a schematic cross-sectional view of a liquid storage tube according to an embodiment of the present invention;

fig. 4 is a schematic front cross-sectional structure view of the main envelope according to the embodiment of the present invention.

In the figure:

1-a centrifugal seat cylinder; 2-preparing a cylinder; 3, sealing a blocking cover; 4-a spacer; 5-a separation cover; 6-a discharge mechanism; 7-a liquid-guiding fetching mechanism;

601-a discharge cylinder; 602-a discharge hole; 603-quantitative water guide sleeve; 604-connecting disc; 605-stretching the column; 606-a primary envelope; 607-guide ring; 608-a polymeric cover; 609-filter ring cover; 610-a support ring; 611-filtering ring; 612-rotating sleeve; 613-through holes; 614-breaking the tablet groups; 615-gravity bar; 616-defragmentation;

701-liquid guiding column tube; 702-liquid feeder; 703-taking the article; 704-liquid separation block; 705-liquid separating tube; 706-leakage cover; 707-threaded post; 708-insulating ring blocks; 709-three-terminal column tube; 710-through guide post; 711-insufflation of the balloon; 712-a liquid storage tube; 713-an arc-shaped chamber; 714-drainage lumen; 715-a conditioning cavity; 716-glue pushing block.

Detailed Description

The present invention is described in terms of particular embodiments, other advantages and features of the invention will become apparent to those skilled in the art from the following disclosure, and it is to be understood that the described embodiments are merely exemplary of the invention and that it is not intended to limit the invention to the particular embodiments disclosed. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

As shown in fig. 1, the present invention provides a device for preparing VC collagen anti-wrinkle essence for preventing skin aging, which uses a quantitative water guide sleeve 603 to achieve quantitative discharge of filtrate, thereby releasing both hands of an operator and reducing the probability of misoperation, and then uses a filtering ring cover 609 to filter, so that the precipitate cannot easily flow away with supernatant, and the raw materials can be stirred and broken during extraction, so that the extraction efficiency of collagen is higher, and the device structure is compact, so that the operator does not need to use other devices during extraction and filtration operation, i.e. the solution does not contact the outside for a long time, thereby reducing the probability of contamination; the device can also realize the quantitative input of liquid through the guide post 710, namely when adding the extraction auxiliary additive, the additive can be slowly blown into the filtrate in the separation cover 5 through the extrusion air bag, the operation is simple and convenient, the input amount can be easily controlled, and the probability of misoperation (such as the probability of excessive liquid input) in the liquid input process is reduced.

As shown in fig. 1, the preparation device comprises a centrifuge holder cylinder 1 and a preparation cylinder 2 sleeved in the centrifuge holder cylinder 1, wherein the top end of the preparation cylinder 2 is connected with a sealing cover 3, the top of the centrifuge holder cylinder 1 can be additionally provided with a device to enable the whole preparation cylinder 2 to be in a sealing state, so that the preparation cylinder 2 is prevented from being damaged during centrifugal operation, meanwhile, the bottom of the centrifuge holder cylinder 1 can be provided with a component for driving the preparation cylinder 2 to rotate at high speed, the specific structure of the preparation device can be set as shown in fig. 1, the preparation cylinder 2 can rotate after a chuck is driven by a motor, the inner wall of the preparation cylinder 2 is hermetically connected with a separation frame 4 with a V-shaped cross section, the outer surface of the bottom of the separation frame 4 is provided with a separation cover 5 with a U-shaped cross section, a certain gap exists between the separation cover 5 and the bottom of the preparation cylinder 2, and the specific structure can be shown in fig. 1, and coolable media or components such as cold water can circulate in the gap so as to enable the whole preparation cylinder 2 to be always in a 4 ℃ area, the other end of the separation cover 5 is hermetically connected with the inner wall of the bottom of the preparation cylinder 2, a discharge mechanism 6 is installed at the central position of the inner wall of the bottom of the separation frame 4, and a liquid guiding and fetching mechanism 7 is connected to the discharge mechanism 6.

When the preparation device is used, pretreated fish scales can be directly placed into a preparation cylinder 2 to be positioned on a separation frame 4 (impurities such as meat, muscle and tendon and the like remained on animal bones are removed during pretreatment, the crushed fish scales are extracted by n-butyl alcohol or n-hexane to obtain bone oil, inorganic matters in the bones are removed to improve the yield of collagen, the mineral matters in the bones can be removed by using dilute acid or EDTA solution, the human raw materials are decalcified for 2d by 1.0mo L/L HC L with 5 times of mass, the human raw materials are degreased by using n-ethane and then are subjected to enzymolysis by pepsin, the enzyme adding amount is 150U/g, the pH value is 1.7, the human raw materials are treated for 120min under the condition of 37 ℃, then the human raw materials are extracted for 5h under the condition that the solid-liquid ratio is 1: 6, the extraction rate can reach 18%, the human raw materials are soaked in an EDTA solution (pH7.4) for 5d to effectively remove hydroxyapatite in aggregates, then acetic acid is added for soaking for 24 hours, then the preparation cylinder 2 is placed into a centrifugal seat cylinder 1 for operation, the subsequent extraction mechanism is operated, the extraction of the aggregate is separated by a centrifugal filter, the filter residue is removed, and the subsequent filter residue is removed by a certain amount after the centrifugal discharge mechanism is removed, and the filter residue removing mechanism is removed, and.

In this embodiment, each component of the manufacturing apparatus can be made of a glass material.

As shown in fig. 1 and 4, the discharging mechanism 6 includes a discharging cylinder 601 whose end is mounted on the inner wall of the bottom of the separating frame 4, in order to make the discharging cylinder 601 have enough hardness, the discharging cylinder 601 can be made of metal material, the other end of the discharging cylinder 601 is connected with the inner wall above the blocking cover 3, a plurality of discharging holes 602 with a splayed cross section are opened on the side wall of the discharging cylinder 601, a quantitative water guiding sleeve 603 is connected with the inner wall of the discharging cylinder 601 through a screw thread, a connecting disc 604 with a leakage hole on the surface is mounted on the inner wall of the quantitative water guiding sleeve 603, a stretching column 605 for pulling the quantitative water guiding sleeve 603 is mounted at the center position of the connecting disc 604, one end of the stretching column 605 penetrates through the blocking cover 3, and the other end of the stretching column 605 extends into the separating cover 5.

The discharging mechanism 6 can realize the quantitative discharge of the filtrate, when in specific operation, once a user needs to discharge the supernatant, only the rotary stretching column 605 is used, then the stretching column 605 carries the connecting disc 604 and the quantitative water guiding sleeve 603 to move together in the discharging cylinder 601, because the discharging cylinder 601 is in threaded connection with the quantitative water guiding sleeve 603, the discharging cylinder 601 can circulate up and down in the quantitative water guiding sleeve 603, but no leakage occurs (the distance between the upper part and the lower part of the discharge cylinder 601 is small, namely the movement amount can be accurately controlled), when one end of the discharge cylinder 601 close to the blocking cover 3 contacts the first discharge hole 602 on the side wall of the discharge cylinder 601, the uppermost layer of the supernatant gradually flows into the quantitative water guide sleeve 603 through the discharge hole 602, so that the supernatant flows into the separation hood 5 through the leakage holes of the connecting disc 604, and then, the user can install the same operation so that the supernatant liquid above the spacer 4 can be filtered away.

The quantitative water guide sleeve 603 comprises a main sleeve 606 connected with the inner wall of the discharge cylinder 601, one end of the main sleeve 606 close to the blocking cover 3 is provided with a guide ring 607 with a splayed cross section, and the other end of the main sleeve 606 is provided with a polymerization cover 608 for polymerizing the filtrate and enabling the filtrate to flow down along the stretching column 605.

After the supernatant passes through the discharge hole 602, the supernatant enters the guide ring 607 first, because the cross section of the guide ring 607 is in a splayed structure, the supernatant can easily enter the guide ring 607 without the supernatant being blocked, and because the inner wall of the guide ring 607 is movably connected with a filtering ring cover 609, the filtering ring cover 609 consists of a support ring 610 connected with the inner wall of the guide ring 607, the support ring 610 can be fully clamped in the guide ring 607 to play a role of supporting the filtering ring 611, one end of the support ring 610 far away from the connecting disc 604 is provided with the filtering ring 611, the thickness value of the filtering ring 611 is arranged in a linearly decreasing trend from the center to the outer side, so after the supernatant enters the guide ring 607, the supernatant can be filtered by the filtering ring 611 to filter out larger filter residues in the liquid for the first time, and because the thickness value of the filtering ring 611 is arranged in a linearly decreasing trend from the center to the outer side, the residue on the whole filter ring 611 does not block all the filter holes but is accumulated on the edge of the filter ring 611, and then the supernatant in the guide ring 607 is gathered by the gathering cap 608 and flows down along the stretching rod 605, and then the supernatant flowing down flows into the partition cap 5.

As shown in fig. 1, a rotating sleeve 612 is sleeved outside the discharge cylinder 601, a through hole 613 is formed in a position of a side wall of the rotating sleeve 612 corresponding to the discharge hole 602, a breaking sheet set 614 is installed on the side wall of the rotating sleeve 612, and a gravity rod 615 is installed at one end, far away from the rotating sleeve 612, of the breaking sheet set 614.

Meanwhile, the discharging mechanism 6 can stir and crush the raw materials during extraction, so that the extraction efficiency of the collagen is higher (the extraction operation is performed between the discharging operations), during the specific operation, a user can put the preparation cylinder 2 into the centrifuge bowl cylinder 1 for a short period of centrifugal operation because the fish scales are soaked above the separating frame 4, once the preparation cylinder 2 is driven, the rotating sleeve 612 can be driven to rotate together, and because the crushing sheet group 614 is arranged on the side wall of the rotating sleeve 612, the crushing sheet group 614 and the gravity rod 615 arranged on the crushing sheet group 614 can be driven by the rotating sleeve 612, so that the crushing sheet group 614 can stir and crush the fish scales above the separating frame 4 with larger rotating force, and the extraction efficiency of the collagen is higher.

The breaking sheet group 614 is composed of a plurality of breaking sheets 616, the length value of the breaking sheets 616 is set in a linear decreasing trend from one end close to the blocking cover 3 to one end far away from the blocking cover 3, and the breaking sheet group 614 is set in such a way so as to enable fish scales to be broken more thoroughly.

As shown in fig. 1, 2 and 3, the liquid guiding fetching mechanism 7 includes a liquid guiding column tube 701 located in the separation hood 5 and hermetically connected to one end of the stretching column 605 and a liquid feeder 702 installed at the other end of the stretching column 605, a material taking device 703 is installed on the side wall of the liquid guiding column tube 701, a liquid distribution block 704 with a triangular cross section is installed in the liquid guiding column tube 701, two liquid distribution tubes 705 with L-shaped structures are hermetically installed at one end of the liquid guiding column tube 701 far from the stretching column 605, the material taking device 703 includes a leakage hood 706 installed on the side wall of the liquid guiding column tube 701 and having a L-shaped structure, and an isolation ring block 708 is installed at one end of the leakage hood 706 close to the stretching column 605.

The liquid guiding and fetching mechanism 7 can realize the quantitative input of liquid, namely when adding the extraction auxiliary additive, the additive can be slowly blown into the filtrate in the separation hood 5 by pressing the air bag, and the filter residue can be quickly taken away by the object taking device 703, so that the subsequent operation is simpler and quicker, when in specific operation, once the extracted auxiliary additive needs to be input, the additive is only injected into the liquid adding device 702, then once the additive passes through the stretching column 605 and the liquid guiding column pipe 701 in sequence, the additive is directly divided into two parts by the liquid dividing block 704, then the two parts of additive enter the liquid dividing pipe 705, the specific structure of the liquid dividing pipe 705 is shown in figures 1 and 2, one liquid dividing pipe 705 is larger, when the additive is added, the additive can be added more uniformly in the separation hood 5, when the liquid flows down along the stretching column 605 to enter the separation hood 5, the liquid directly enters the object leaking hood 706, and then passes through the object leaking hole on the object leaking hood 706, filter residues in the liquid are located in the object leakage cover 706, then the filter liquid in the object leakage cover 706 is driven once the separation cover 5 is driven to rotate, and after the filter liquid is driven, the filter liquid can be isolated and blocked through the isolation ring block 708, so that the filter residues in the object leakage cover 706 cannot be easily thrown out.

As shown in fig. 1, 2 and 3, the liquid feeder 702 comprises a three-terminal pillar 709 with one end hermetically connected to a tension pole 605, a guiding pole 710 hermetically installed at the other end of the three-terminal pillar 709, an insufflation balloon 711 which is hermetically connected to the three-port column tube 709 and used for insufflation of the liquid in the guide column 710 into the tension column 605 is provided above the guide column 710, the through guide column 710 includes a liquid storage tube 712 hermetically connected to the three-terminal column tube 709 and having a U-shaped structure, an arc-shaped cavity 713 is arranged in the liquid storage pipe 712, one end of the arc-shaped cavity 713 is provided with a liquid guide cavity 714 which is communicated with the three-end column pipe 709 and has an isosceles trapezoid structure in cross section, the other end of the arc-shaped cavity 713 is provided with an adjusting cavity 715, and a rubber pushing block 716 is connected in the adjusting cavity 715, and a threaded column 707 penetrating the adjusting cavity 715 to the outer side is installed on one side surface of the rubber pushing block 716 away from the arc-shaped cavity 713.

After the additive is put into the liquid feeder 702, the additive is directly injected into the guiding column 710 (as shown in fig. 2 and 3, the additive introduced into the guiding column 710 enters the arc-shaped cavity 713, and in order to enable the amount of the liquid in the guiding column 710 to be freely and accurately controlled, scales can be arranged on the side wall of the guiding column 710), then the liquid injected into the guiding column 710 pushes the rubber pushing block 716 and the threaded column 707 to move together until the amount of the liquid reaches the set measurement, then the air bag 711 can be squeezed and blown in once the liquid is required to be put into, at this time, the air bag 711 can blow air to reduce the pressure in the stretching column 605, then the liquid in the guiding column 710 is sent into the stretching column 605 (because the cross section of the guiding chamber 714 is in an isosceles trapezoid structure, the liquid in the guiding chamber 714 can easily enter the stretching column 605), and then the liquid in the stretching column 605 flows into the guiding column pipe 701, thereby being divided into two by the liquid dividing block 704.

In step S100, the preparation cylinder is placed in a centrifuge bowl for high-speed centrifugation operation, so that the efficiency of distant protein extraction is higher, and the specific operation steps are as follows:

Although the invention has been described in detail above with reference to a general description and specific examples, it will be apparent to one skilled in the art that modifications or improvements may be made thereto based on the invention. Accordingly, such modifications and improvements are intended to be within the scope of the invention as claimed.

Claims

1. The preparation device is characterized by comprising a centrifugal seat cylinder (1) and a preparation cylinder (2) sleeved in the centrifugal seat cylinder (1), wherein the top end of the preparation cylinder (2) is connected with a blocking cover (3), the inner wall of the preparation cylinder (2) is hermetically connected with a separation frame (4) with a V-shaped cross section, the outer surface of the bottom of the separation frame (4) is provided with a separation cover (5) with a U-shaped cross section, the other end of the separation cover (5) is hermetically connected with the inner wall of the bottom of the preparation cylinder (2), the central position of the inner wall of the bottom of the separation frame (4) is provided with a discharge mechanism (6), and the discharge mechanism (6) is connected with a liquid guide fetching mechanism (7);

arrange material mechanism (6) and include that the tip is installed in row material section of thick bamboo (601) of separating frame (4) bottom inner wall, arrange the other end of material section of thick bamboo (601) with the top inner wall connection of closing off lid (3), just arrange the lateral wall of material section of thick bamboo (601) and offer a plurality of transversal relief hole (602) of personally submitting the splayed structure arrange the inside wall of material section of thick bamboo (601) and have quantitative water guide sleeve (603) through threaded connection the inner wall of quantitative water guide sleeve (603) is installed connection pad (604) that the surface was seted up the small opening the central point department of putting of connection pad (604) installs tensile post (605) that is used for pulling quantitative water guide sleeve (603), draw post (605) one end to run through closing off lid (3), just draw the other end of stretching post (605) to extend to in separating cover (5).

2. The device for preparing the VC collagen wrinkle-resistant essence for preventing skin aging according to claim 1, wherein the quantitative water guide sleeve (603) comprises a main sleeve (606) connected with the inner wall of the discharge cylinder (601), one end of the main sleeve (606) close to the blocking cover (3) is provided with a guide ring (607) with a splayed cross section, and the other end of the main sleeve (606) is provided with a polymerization cover (608) for polymerizing the filtrate and enabling the filtrate to flow down along the stretching column (605).

3. The preparation device of the VC collagen anti-wrinkle essence for preventing skin aging according to claim 2, wherein a filter ring cover (609) is movably connected to the inner wall of the guide ring (607), the filter ring cover (609) is composed of a support ring (610) connected to the inner wall of the guide ring (607), a filter ring (611) is installed at one end of the support ring (610) far away from the connecting disc (604), and the thickness value of the filter ring (611) is set in a linearly decreasing trend from the center to the outer side.

4. The device for preparing the VC collagen anti-wrinkle essence for preventing skin aging according to claim 1, wherein a rotating sleeve (612) is sleeved on the outer side of the discharge cylinder (601), a through hole (613) is formed in the position of the side wall of the rotating sleeve (612) corresponding to the discharge hole (602), a breaking sheet set (614) is installed on the side wall of the rotating sleeve (612), and a gravity rod (615) is installed at one end, far away from the rotating sleeve (612), of the breaking sheet set (614).

5. The device for preparing the VC collagen anti-wrinkle essence for preventing skin aging according to claim 4, wherein the breaking sheet set (614) is composed of a plurality of breaking sheets (616), and the length values of the plurality of breaking sheets (616) are set in a linearly decreasing trend from the end close to the blocking cover (3) to the end far away from the blocking cover (3).

6. The device for preparing the VC collagen anti-wrinkle essence for preventing skin aging according to claim 1, wherein the liquid guiding and fetching mechanism (7) comprises a liquid guiding column tube (701) which is positioned in the separation cover (5) and is hermetically connected with one end of the stretching column (605), and a liquid throwing device (702) which is arranged at the other end of the stretching column (605), the side wall of the liquid guiding column tube (701) is provided with a material taking device (703), a liquid distributing block (704) with a triangular cross section is arranged in the liquid guiding column tube (701), one end of the liquid guiding column tube (701) far away from the stretching column (605) is hermetically provided with two liquid distributing tubes (705) with L type structures, the material taking device (703) comprises a leakage cover (706) which is arranged at the side wall of the liquid guiding column tube (701) and has a L type structure, and one end of the leakage cover (706) close to the stretching column (605) is provided with an isolation ring block (708).

7. The device for preparing the VC collagen anti-wrinkle essence for preventing skin aging according to claim 6, wherein the liquid feeder (702) comprises a three-terminal column tube (709) with one end hermetically connected with the stretching column (605), the other end of the three-terminal column tube (709) is hermetically provided with a through guide column (710), and an insufflation air bag (711) which is hermetically connected with the three-terminal column tube (709) and is used for blowing the liquid in the through guide column (710) into the stretching column (605) is arranged above the through guide column (710).

8. The device for preparing the VC collagen anti-wrinkle essence capable of preventing skin aging according to claim 7, wherein the guide post (710) comprises a liquid storage tube (712) which is hermetically connected with a three-terminal column tube (709) and has a U-shaped structure, an arc-shaped cavity (713) is arranged in the liquid storage tube (712), a liquid guide cavity (714) which is communicated with the three-terminal column tube (709) and has an isosceles trapezoid-shaped cross section is arranged at one end of the arc-shaped cavity (713), an adjusting cavity (715) is arranged at the other end of the arc-shaped cavity (713), a rubber push block (716) is connected in the adjusting cavity (715), and a threaded post (707) which penetrates through the adjusting cavity (715) to the outer side is arranged on the surface of one side of the rubber push block (716) far away from the arc-shaped cavity (713).

9. A preparation method of VC collagen anti-wrinkle essence capable of preventing skin aging is characterized by comprising the following steps:

10. The method for preparing VC collagen anti-wrinkle essence capable of preventing skin aging as claimed in claim 9, wherein in step S100, the preparation cylinder is placed in a centrifuge bowl to perform high-speed centrifugation operation, so that the efficiency of distant protein extraction is higher, and the specific operation steps are as follows: