CN111458322A - Method and device for detecting fluorescent pollutants of intelligent equipment - Google Patents
Method and device for detecting fluorescent pollutants of intelligent equipment Download PDFInfo
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- CN111458322A CN111458322A CN202010492596.7A CN202010492596A CN111458322A CN 111458322 A CN111458322 A CN 111458322A CN 202010492596 A CN202010492596 A CN 202010492596A CN 111458322 A CN111458322 A CN 111458322A
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- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/6456—Spatial resolved fluorescence measurements; Imaging
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Abstract
The invention belongs to the technical field of dye detection, and relates to a method and a device for detecting fluorescent pollutants of intelligent equipment. The invention firstly proposes to combine fluorescent pollutant detection with intelligent equipment, control a fluorescence excitation light source to irradiate a detected object and excite an organic pollutant to emit fluorescence through intelligent equipment application software, synchronously start an intelligent equipment camera to collect image information of the detected object after being filtered by a light filter, and analyze and process the collected image information to obtain the position and concentration information of the pollutant. The intelligent device is applied to the detection of the organic pollutants, the camera of the intelligent device is used for collecting the fluorescence image of the detected object, no additional camera is needed to be configured, meanwhile, the image information of the detected object is analyzed and processed in real time by utilizing the strong data processing capacity of the intelligent device, the intelligent device has the remarkable advantages of low cost, convenience in carrying, high timeliness and the like, and a user can detect the pollutants in real time anytime and anywhere.
Description
Technical Field
The invention belongs to the technical field of pollutant detection, and can be used for conveniently detecting organic pollutants in food and environment in real time by combining intelligent equipment.
Background
With the continuous improvement of living standard of people, more and more people are becoming aware of the importance of health, and the food safety and environmental sanitation problems are becoming the focus of people's attention in life. In daily life of people, organic pollutants are very common pollutants, such as feces pollutants, organic pesticide residue pollutants, pathogenic bacteria pollutants such as escherichia coli O157: H7, salmonella, listeria and the like, and are various and wide in distribution range, so that environmental sanitation is damaged, food poisoning frequently occurs, virus propagation is difficult to control, and life health of people is seriously influenced. The timely discovery and removal of organic pollutants play an extremely important role in guaranteeing food safety, environmental sanitation and reducing the spread rate of diseases. In addition, organic pollutants on the surface of the food can be removed in time, so that the storage time of the food can be prolonged, the waste in a food supply chain is reduced, and the whole ecological environment is improved. However, slight organic contaminants on the surface of food and in the environment are easily overlooked because they are not readily detectable to the naked eye. Therefore, how to identify organic pollutants quickly, efficiently and conveniently becomes a very much concern in daily life of people.
The method for identifying the organic pollutants by adopting the chromatographic separation detection technology is a traditional organic pollutant detection method, but the method needs to depend on large-scale equipment, is complex to operate and long in detection time, and is not suitable for field detection. In addition, the method needs to use an organic solvent in the detection process, and is easy to cause secondary environmental pollution. In recent years, microscopic imaging techniques and spectroscopic detection techniques have been used to detect organic contaminants. The organic pollutant detection method based on the microscopic imaging technology determines the polluted condition by observing the pollutant loading capacity on the surface of an object to be detected through microscopic imaging. Because the field of view of the microscope is very small, in order to comprehensively detect the pollution condition of the detected object, the detected object needs to be scanned and imaged, so the method has the advantages of low detection speed, complex detection process and expensive and complex equipment. The organic pollutant detection based on the spectrum detection technology comprises a fluorescence spectrum detection technology, a mid-infrared detection technology, a Raman spectrum detection technology and the like. The technologies have the advantages of high detection speed, high precision, no damage to samples and the like, and are hot spots of research in the field at present. However, the detection equipment required by these techniques is complex in structure and expensive, and therefore is only suitable for laboratory sampling detection.
The laboratory test devices described above are time consuming, labor intensive and inefficient for the average consumer, and therefore are completely unsuitable for use by the average consumer. For common consumers, the organic pollutant detection equipment which can be conveniently carried and has the rapid real-time detection capability is urgently expected by the common consumers, the selected products can be rapidly detected and analyzed at any time when foods such as vegetables and fruits are purchased, and the sanitary conditions of the hotel can be rapidly checked when the users live in the hotel.
Disclosure of Invention
In order to solve the problems, the invention provides a method and a device for detecting fluorescent pollutants of intelligent equipment. Aiming at the problems of poor detection timeliness, extremely low applicability of common consumers and the like caused by high cost and heavy volume of the existing organic pollutant detection equipment, the intelligent organic pollutant fluorescence detection equipment combines intelligent equipment and an organic pollutant fluorescence detection technology, has the remarkable advantages of low cost, convenience in carrying, high timeliness and the like, and can be used for detecting pollutants in real time at any time and any place by a user.
On one hand, the invention provides a method for detecting fluorescent pollutants of intelligent equipment, which is characterized by comprising the following steps: the intelligent device application software sends an instruction to the fluorescence excitation light source module through the communication module; the fluorescence excitation light source module emits excitation light to irradiate the measured object after receiving the instruction, and the image of the measured object is imaged on an image sensor of the intelligent equipment camera by a lens of the intelligent equipment camera after filtering the optical information of the excitation light wave band by the optical filter; meanwhile, the intelligent device application software controls an image sensor of the intelligent device camera to acquire image information of the measured object; after the image sensor of the intelligent equipment camera collects the image information of the measured object, the intelligent equipment application software sends an instruction to the fluorescence excitation light source module through the communication module to control the fluorescence excitation light source module to stop emitting excitation light; and the intelligent device application software analyzes the pollutant information on the surface of the measured object through the acquired image information of the measured object.
On the other hand, the invention provides a fluorescent pollutant detection device for intelligent equipment, which comprises the intelligent equipment and is characterized in that: the device comprises a communication module, a fluorescence excitation light source module and an optical filter; the intelligent device comprises intelligent device application software, a lens of an intelligent device camera and an image sensor of the intelligent device camera, wherein the intelligent device application software is used for controlling the fluorescence excitation light source module to emit light, controlling the image sensor of the intelligent device camera to acquire image information of a measured object and analyzing pollutant information on the surface of the measured object; the communication module is used for transmitting instructions between the intelligent device application software and the fluorescence excitation light source module; the fluorescence excitation light source module comprises a fluorescence excitation light source, a light source shaping lens, a light source driving circuit and a power supply, wherein the fluorescence excitation light source is used for emitting excitation light for realizing fluorescence detection on a detected object, and the light source shaping lens is used for adjusting the irradiation area and the light beam uniformity degree of the fluorescence excitation light so as to prevent the problems of low fluorescence excitation efficiency caused by over-diffusion of the energy of the excitation light and poor detection response consistency caused by uneven irradiation of the laser light source; the optical filter is used for filtering light information of the excitation light wave band, and the excitation light is prevented from entering an image sensor of the intelligent equipment camera to interfere with the identification of useful fluorescence information.
Compared with the prior art, the invention has the following innovation points and remarkable advantages:
1. the intelligent device is applied to the detection of the organic pollutants, the camera carried by the intelligent device is used for collecting the fluorescence image of the detected object, and an additional camera is not required to be configured, so that the device is greatly simplified, the system cost is reduced, and the intelligent device is convenient for a user to carry;
2. the invention utilizes the powerful image shooting function and data processing capacity of the intelligent equipment to detect the organic pollutants, and can accurately and quickly obtain the areas occupied by the pollutants and the concentration information of the pollutants;
3. according to the invention, by utilizing the powerful visual expression capability of the intelligent equipment, the pollutant position information and the concentration information can be clearly displayed on the screen of the intelligent equipment, and an auxiliary indication function is added, so that a user can conveniently and rapidly position the pollutant and take action;
4. according to the invention, application software is developed on the intelligent equipment, the light-emitting time sequence of the fluorescence excitation light source module is accurately controlled, the synchronization between the fluorescence excitation light source module and the shooting of a camera of the intelligent equipment in a pulse working mode is ensured, the power consumption of a system can be greatly reduced, and the endurance time is increased;
5. the invention provides a method for directly supplying power to a fluorescence excitation light source module by using a power supply module of intelligent equipment, thereby further reducing the complexity and cost of the system and improving the portability of the system;
6. the invention also provides a function of using the power supply in the detection device to supply power to other equipment as a mobile power supply, thereby meeting the multidimensional use requirements of users.
Drawings
FIG. 1 is a schematic diagram of a method for detecting fluorescent contaminants in a smart device according to the present invention;
FIG. 2 is a schematic diagram of a fluorescent contaminant detection device of the smart device of the present invention;
FIG. 3 is a schematic diagram of a fluorescent contaminant detection device in which the smart device of the present invention is a smart phone;
FIG. 4 is a schematic diagram of a fluorescent contaminant detection device of the present invention, in which the intelligent device is a tablet computer
FIG. 5 is a schematic diagram of an embodiment of the detection of fluorescent pollutants in a smart device based on USB communication according to the present invention;
FIG. 6 is a schematic diagram of a fluorescent contaminant detection device of an intelligent device according to the present invention for supplying power to other devices;
FIG. 7 shows the result of detecting organic contaminants on the surface of fruits according to the present invention;
1-intelligent equipment, 2-communication module, 3-fluorescence excitation light source module, 4-optical filter, 5-measured object, 6-intelligent mobile phone, 7-measured fruit image when excitation light is emitted, 8-measured fruit image when excitation light is not emitted, 9-fruit surface pollutant area, 10-tablet computer, 101-intelligent equipment application software, 102-intelligent equipment camera, 103-intelligent equipment image sensor, 104-intelligent equipment lens, 201-Bluetooth communication module, 202-USB communication module, 301-fluorescence excitation light source, 302-light source shaping lens, 303-light source driving circuit, 304-power supply, 305-L light source, ED 306-lithium battery, 601-intelligent mobile phone application software, 602-intelligent mobile phone camera, 603-intelligent mobile phone image sensor, 604-intelligent mobile phone lens, 1001-tablet computer application software, 1002-tablet computer camera, 1003-tablet computer image sensor and 1004-tablet computer lens.
Detailed Description
The invention is further illustrated by the following figures and examples.
The invention perfectly combines the fluorescent organic pollutant detection technology with intelligent equipment, can realize the rapid real-time detection of the organic pollutants on the surface of the detected object, has the advantages of convenient carrying and low cost, and is very suitable for common consumers. The practice of the present invention is illustrated in detail by the following examples.
Example 1
The device for detecting the fluorescent pollutants of the smart device constructed in the embodiment is shown in fig. 2 and 3, the smart device 1 used in the embodiment is a smart phone 6, a communication module 2, a fluorescence excitation light source module 3 and a filter 4 are also used in the device, wherein the smart phone 6 comprises smart phone application software 601, an image sensor 603 of a smart phone camera 602 and a lens 604 of the smart phone camera 602, the smart phone application software 601 is used for controlling the luminescence of a fluorescence excitation light source module 3 and the image sensor 603 of the smart phone 6 camera 602 to collect image information of a measured object 5 and analyze the information of the pollutants on the surface of the measured object 5, the lens 604 of the smart phone camera 602 is used for imaging the measured object 5 on the image sensor 603 of the smart phone camera 602, the image sensor 603 of the smart phone camera 602 is used for collecting image information of the measured object 5, the communication module 2 is a bluetooth communication module for transmitting an instruction between the smart phone application software 601 and the fluorescence excitation light source module 3, the communication module 601 and the fluorescence excitation light source module 3 is used for preventing a fluorescence excitation light source module 304 nm energy band from interfering with a fluorescence excitation light source 304 nm, the excitation light source system, the excitation light source 304 nm, the excitation light source 100 nm detection is used for detecting the following specific excitation light source 100 nm, the detection process, the detection is realized by using a fluorescence excitation light source 100 nm detection wavelength detection system, the following steps:
the smart phone application software 601 sends a light emitting instruction to the fluorescence excitation light source module 3 through the bluetooth communication module 201, the fluorescence excitation light source module 3 receives the instruction and then drives L ED light source 305 to emit excitation light with the wavelength of 450 nm and the bandwidth of 10 nm through the light source driving circuit 303, the excitation light is condensed through the light source shaping lens 302 and then irradiates the measured object 5, an image of the measured object 5 is filtered by the optical filter 4 to remove light information with the wavelength less than 465nm and then is imaged on the image sensor 603 of the smart phone camera 602 through the lens 604 of the smart phone camera 602, the smart phone application software 601 sends the light emitting instruction and simultaneously controls the image sensor 603 of the smart phone camera 602 to acquire image information of the measured object 5, after the image sensor 603 of the smart phone camera 602 acquires the image information of the measured object 5, the smart phone application software 601 sends a light emitting stopping instruction to the fluorescence excitation light source module 3 through the bluetooth communication module 201, the fluorescence excitation light source module 3 stops emitting the excitation light after receiving the instruction and then analyzes the acquired image information of the measured object 5 to obtain the pollutant.
According to the embodiment, the smart phone application software 601 records the time for starting to emit pulsed light, the pulse length is set to be 5 μ s and is larger than the minimum exposure time 4 μ s of the image sensor 603 of the smart phone camera 602, the smart phone camera 602 is synchronously started to shoot the image information of the measured object 5 filtered by the optical filter 4 at the same time for emitting the pulsed light, so that L ED light source 305 of the image sensor 603 of the smart phone camera 602 is strictly ensured to be in a light-emitting state when shooting an image, the power consumption of a system is greatly reduced by adopting a mode of emitting the pulsed light, and the endurance time of the power supply 304 is increased.
The smartphone camera 602 in this embodiment respectively captures images of the object 5 to be measured when the fluorescence excitation light source module 3 emits 450 nm violet light and does not emit 450 nm violet light; then, the smartphone application software 601 processes the two photographed images to obtain fluorescence intensity information corresponding to each position point of the object 5 to be measured, and obtains the area occupied by the pollutants and the concentration information of the pollutants according to the fluorescence intensity information of each position point, which are reflected in the embodiment, that is, the position information and the degree information of the rotting of the fruit. As shown in fig. 7, the area 9 of contamination and the degree of contamination on the surface of the fruit is obtained by processing the image 7 of the fruit being measured when the excitation light is emitted and the image 8 of the fruit being measured when the excitation light is not emitted.
Example 2
As shown in fig. 4, unlike embodiment 1, in the intelligent device fluorescent contaminant detection apparatus constructed in this embodiment, the intelligent device 1 is a tablet pc 10, the tablet pc 10 includes a tablet pc application 1001, an image sensor 1003 of a tablet pc camera 1002 and a lens 1004 of the tablet pc camera 1002, the tablet pc application 1001 controls the fluorescence excitation light source module 3 to emit light, the image sensor 1003 of the tablet pc camera 1002 acquires image information of the measured object 5 and analyzes contaminant information on the surface of the measured object 5, the lens 1004 of the tablet pc camera 1002 is used for imaging the measured object 5 on the image sensor 1003 of the tablet pc camera 1002, and the image sensor 1003 of the tablet pc camera 1002 is used for acquiring image information of the measured object 5.
Example 3
Different from the embodiment 1, the optical filter 4 in the intelligent device fluorescent pollutant detection apparatus constructed in this embodiment is a tunable optical filter, and the fluorescence image of the measured object 5 captured by the intelligent device camera 102 is changed by changing the optical wave transmission wavelength of the tunable optical filter. Every time the wavelength is changed, the corresponding intelligent device camera 102 shoots a fluorescence image of the measured object 5, so that a series of fluorescence spectrum images of the measured object 5 with different wavelengths are obtained, and the type of the pollutant is more accurately judged according to the fluorescence spectrum information.
Example 4
Different from embodiment 3, in the intelligent device fluorescent pollutant detection apparatus constructed in this embodiment, a series of measured object fluorescent spectrum images of different wavelengths of the measured object 5 acquired by the smartphone camera 602 are analyzed and processed by using an artificial intelligence algorithm, so that information such as pollutant types, distribution areas, concentrations and the like can be extracted more accurately and quickly.
Example 5
As shown in fig. 5, unlike embodiment 1, the smart phone 6 in the fluorescent pollutant detection device of the smart device constructed in this embodiment communicates with the fluorescence excitation light source module 3 through the USB communication module 202. The smart device application software 101 sends a relevant instruction to the fluorescence excitation light source module 3 through the USB communication module 202, and the transmission stability can be improved by using USB communication in this embodiment.
Example 6
Different from embodiment 5, the fluorescence excitation light source module 3 of the intelligent device fluorescence pollutant detection apparatus constructed in this embodiment does not include the lithium battery 306, in this embodiment, the fluorescence excitation light source module 3 is directly powered by the way that the lithium battery inside the intelligent device is configured with the super capacitor through the USB data line, and the fluorescence excitation light source module 3 is powered by switching on and off of the charge and discharge switch, so that the complexity and cost of the system are reduced, and the whole system is more convenient.
Example 7
As shown in fig. 6, different from embodiment 1, the lithium battery 306 in the fluorescence excitation light source module 3 of the intelligent device fluorescence pollutant detection apparatus constructed in this embodiment supplies power to other electronic devices such as a mobile phone and a tablet computer through the USB communication module 202, so that the versatility of the apparatus is increased, and the apparatus can be used as a mobile power supply while meeting the power supply requirement of the detection system, thereby meeting the multidimensional requirement of the user.
While the invention has been described in connection with specific embodiments thereof, it will be understood that these should not be construed as limiting the scope of the invention, which is defined in the appended claims, any modifications to which this invention pertains being applicable being within the scope of the invention defined in the following claims.
Claims (10)
1. The method for detecting the fluorescent pollutants of the intelligent equipment is characterized by comprising the following steps: the intelligent device application software sends an instruction to the fluorescence excitation light source module through the communication module; the fluorescence excitation light source module emits excitation light to irradiate a measured object after receiving an instruction, and an image of the measured object is imaged on an image sensor of the intelligent equipment camera by a lens of the intelligent equipment camera after optical information of an excitation light wave band is filtered by an optical filter; meanwhile, the intelligent equipment application software controls an image sensor of the intelligent equipment camera to acquire image information of the measured object; after the image sensor of the intelligent equipment camera collects the image information of the measured object, the intelligent equipment application software sends an instruction to the fluorescence excitation light source module through the communication module to control the fluorescence excitation light source module to stop emitting excitation light; and the intelligent device application software analyzes the pollutant information on the surface of the measured object through the acquired image information of the measured object.
2. The intelligent device fluorescent contaminant detection method of claim 1, wherein: the intelligent device is one of a smart phone and a tablet personal computer.
3. The intelligent device fluorescent contaminant detection method of claim 1, wherein: the light emitted by the fluorescence excitation light source module is pulse light, the starting time and the pulse length of the pulse light are strictly synchronized through intelligent equipment application software, and the fluorescence excitation light source module is ensured to be in a light-emitting state when an image sensor of the intelligent equipment camera shoots an image.
4. The intelligent device fluorescent contaminant detection method of claim 1 or 3, wherein: the intelligent equipment camera respectively shoots images of the measured object when the fluorescence excitation light source module emits excitation light and does not emit excitation light; then, the intelligent device application software processes two images respectively shot when the fluorescence excitation light source module emits excitation light and does not emit excitation light to obtain fluorescence intensity information corresponding to each position point of the measured object, and the area occupied by the pollutants and the concentration information of the pollutants are obtained through the fluorescence intensity information of each position point.
5. The intelligent device fluorescent contaminant detection method of claim 1 or 3, wherein: the optical filter is a tunable optical filter, a series of fluorescence spectrum images of the measured object with different wavelengths are obtained by changing the light wave transmission wavelength of the tunable optical filter and combining the fluorescence excitation light source module and the intelligent equipment camera for shooting, and then the type of the pollutant is more accurately judged.
6. The intelligent device fluorescent contaminant detection method of claim 1 or 5, wherein: and analyzing the acquired image information of the detected object by using an artificial intelligence-based algorithm, and extracting pollutant information.
7. Intelligent device fluorescence pollutant detection device, including intelligent device, its characterized in that: the device comprises a communication module, a fluorescence excitation light source module and an optical filter; the intelligent device comprises intelligent device application software, a lens of an intelligent device camera and an image sensor of the intelligent device camera, wherein the intelligent device application software is used for controlling the fluorescence excitation light source module to emit light, controlling the image sensor of the intelligent device camera to acquire image information of a measured object and analyzing pollutant information on the surface of the measured object, the lens of the intelligent device camera is used for imaging the measured object on the image sensor of the intelligent device camera, and the image sensor of the intelligent device camera is used for acquiring the image information of the measured object; the communication module is used for transmitting instructions between the intelligent device application software and the fluorescence excitation light source module; the fluorescence excitation light source module comprises a fluorescence excitation light source, a light source shaping lens, a light source driving circuit and a power supply, the power supply is charged by using an external power supply, the fluorescence excitation light source is used for emitting excitation light for realizing fluorescence detection on a detected object, and the light source shaping lens is used for adjusting the irradiation area and the light beam uniformity degree of the fluorescence excitation light so as to prevent the problems of low fluorescence excitation efficiency caused by over diffusion of the energy of the excitation light and poor detection response consistency caused by uneven irradiation of a laser light source; the optical filter is used for filtering light information of the excitation light wave band, and prevents the excitation light from entering an image sensor of the intelligent equipment camera to interfere with the identification of useful fluorescence information.
8. The smart device fluorescent contaminant detection assembly of claim 7, wherein: the communication module is a wireless communication module.
9. The smart device fluorescent contaminant detection assembly of claim 7, wherein: and removing the power supply of the fluorescence excitation light source module, and directly supplying power to the fluorescence excitation light source module by the power supply of the intelligent equipment.
10. The smart device fluorescent contaminant detection assembly of claim 7, wherein: and the power supply in the fluorescence excitation light source module is used for supplying power to the outside and providing a charging power supply for other equipment.
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| CN202010492596.7A CN111458322A (en) | 2020-06-03 | 2020-06-03 | Method and device for detecting fluorescent pollutants of intelligent equipment |
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| CN202010492596.7A CN111458322A (en) | 2020-06-03 | 2020-06-03 | Method and device for detecting fluorescent pollutants of intelligent equipment |
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