CN111378692A - Method for extracting inositol from rice bran and preparing disodium hydrogen phosphate - Google Patents
Method for extracting inositol from rice bran and preparing disodium hydrogen phosphate Download PDFInfo
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- CN111378692A CN111378692A CN201811637541.XA CN201811637541A CN111378692A CN 111378692 A CN111378692 A CN 111378692A CN 201811637541 A CN201811637541 A CN 201811637541A CN 111378692 A CN111378692 A CN 111378692A
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- C01B25/30—Alkali metal phosphates
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Abstract
The invention provides a preparation method of inositol, which specifically comprises a) a step of contacting a component A containing phytic acid with ion exchange resin to obtain ion exchange resin B adsorbing phytic acid; b) contacting the ion exchange resin B adsorbed with phytic acid with phytase; c) a step of obtaining a liquid phase C; and d) a step of separating the inositol obtained from the liquid phase C. The purity of the inositol prepared by the invention can reach more than 98 percent. The invention also provides a preparation method of the disodium hydrogen phosphate, which specifically comprises a) a step of contacting the component A containing phytic acid with ion exchange resin to obtain ion exchange resin B adsorbing phytic acid; b) contacting the ion exchange resin B adsorbed with phytic acid with phytase; e) a step of obtaining a solid phase D; and f) separating and obtaining disodium hydrogen phosphate and hydrates thereof from the solid phase D. The purity of the disodium hydrogen phosphate hydrate prepared by the method can reach more than 98%.
Description
Technical Field
The invention relates to the field of fine chemical engineering, and in particular relates to extraction and preparation of inositol and disodium hydrogen phosphate.
Background
China is a large country for rice production, rice bran is a byproduct of rice processing, the rice bran produced in China in the current year is about l000 ten thousand tons, except that a few rice bran is used for extracting the sweetberry oil, most of the rice bran and the degreased rice bran after oil extraction are wasted due to insufficient development and utilization, or the degreased rice bran is only used as simple livestock feed, contains protein, saccharides and cellulose, contains rich phytate with the content of about 9-14%, and mainly exists in a mixture form of Ca, Mg and K. The metal ion removed from the phytate is phytic acid, which is formed by combining inositol (Myo-inositol) with six phosphoric acids, and the phytic acid usually forms a tight combination with protein in seeds, thereby influencing the utilization of the protein; and it can also chelate with monovalent and bivalent ions to reduce the bioavailability of mineral elements, so phytate is an anti-nutritional factor, but inositol after removing phosphate is a biological promoting factor, and is widely applied in medicine, food and feed industries.
Inositol is a saturated cyclic polyol, namely hexahydroxycyclohexane, has the effects similar to vitamin B1 and vitamin H, and is a low molecular organic substance indispensable for human and animals to maintain normal physiological functions. In recent years, inositol has been widely used in the fields of food industry, pharmaceutical industry, feed industry, and the like. Inositol is often used as a nutrient supplement in the food industry. The daily requirement of human bodies for inositol is 1-2 g, and a plurality of health-care beverages and children's foods are added with a trace amount of inositol. Inositol in the pharmaceutical industry is mainly used for treating diseases such as liver cirrhosis, fatty liver, angiosclerosis, high cholesterol in blood, carbon tetrachloride poisoning and the like. At present, most domestic and foreign inositol manufacturers adopt a pressurized hydrolysis process, the process is operated at high temperature and high pressure, the energy consumption is high, the operation is inconvenient, and impurities such as protein, fat, starch and the like are remained in hydrolysate after being hydrolyzed with calcium phytate, so that the refining difficulty is increased, and the yield of inositol is influenced. There are also reports in the literature that inositol is obtained by hydrolyzing calcium phytate with a biological enzyme, such as the study on extracting inositol by hydrolyzing calcium phytate with a biological enzyme, but the purification process after hydrolysis is complicated.
The food additive disodium hydrogen phosphate is one of the most widely applied food quality improving agents in various countries in the world at present, is widely applied to various fields of processing type foods, and plays an important role in improving the food quality. The action mechanism of the food additive is to realize the functions of improving the appearance and the touch of food in food processing by water retention, moisture retention, filling, plasticization, emulsification, compatibilization and improvement of rheological property.
The invention adopts a novel enzymatic hydrolysis process, the phytate in the rice bran is hydrolyzed by the enzymatic method to obtain the inositol, and the residual phosphate radical in the phytic acid is partially converted into the disodium hydrogen phosphate.
Disclosure of Invention
In a first aspect, the present invention provides a process for the preparation of inositol comprising:
a) contacting an aqueous solution containing phytic acid with an ion exchange resin A to obtain an ion exchange resin B absorbed with phytic acid;
b) contacting ion exchange resin B adsorbed with phytic acid with phytase and water
c) A step of obtaining a liquid phase C; and
d) separating the inositol from the liquid phase C.
In a particular embodiment, prior to step a) comprises:
a1) contacting rice bran with enzyme to obtain rice bran zymolyte; and
a2) treating the rice bran zymolyte, separating and obtaining the aqueous solution containing the phytic acid.
In a specific embodiment, in step a1), contacting rice bran with amylase and/or protease to obtain a rice bran hydrolysate;
in a particular embodiment, the amylase is selected from at least one of α amylase, β amylase, gamma amylase, and isoamylase;
in a specific embodiment, the protease is selected from at least one of an alkaline protease, an acidic protease and a neutral protease;
in a specific embodiment, the amylase and/or protease is/are used in an amount of 0.1-0.5% by mass of rice bran;
in a particular embodiment, the rice bran is defatted rice bran and/or non-defatted rice bran.
In a specific embodiment, the step a2) comprises the steps of acid-treating the rice bran zymolyte, separating to obtain an aqueous solution containing phytic acid;
in a specific embodiment, further comprises the step of separating and removing the liquid phase of the rice bran hydrolysate;
in a specific embodiment, the rice bran hydrolysate liquid phase is removed by centrifugation;
in a specific embodiment, the rice bran hydrolysate liquid phase is removed by centrifugation to obtain a rice bran hydrolysate solid phase;
in a specific embodiment, the acid treatment of the rice bran enzymatic hydrolysate comprises suspending a rice bran enzymatic hydrolysate solid phase in water to obtain a rice bran enzymatic hydrolysate suspension, and adjusting the pH of the rice bran enzymatic hydrolysate suspension;
in a specific embodiment, the ratio of the solid phase of the rice bran hydrolysate to the water in the rice bran hydrolysate suspension is 1:6-1: 10;
in a specific embodiment, the ratio of the solid phase of the rice bran hydrolysate to the water in the rice bran hydrolysate suspension is 1:6-1: 8;
in a specific embodiment, the ratio of the solid phase of the rice bran hydrolysate to the water in the rice bran hydrolysate suspension is 1:7.5-1: 8.5;
in a specific embodiment, the adjusting the pH of the rice bran hydrolysate suspension is 1-3;
in a particular embodiment, the acid treatment of the rice bran enzymatic hydrolysate comprises contacting the rice bran enzymatic hydrolysate solid phase with an acid solution a;
in a specific embodiment, the pH of acid solution a is 1-3;
in a specific embodiment, the pH of acid solution a is 1.5-2.5;
in a specific embodiment, the amount of the acid solution a is 6-10 times of the solid phase mass of the rice bran zymolyte;
in a specific embodiment, the amount of the acid solution a is 6-8 times of the solid phase mass of the rice bran zymolyte;
in a specific embodiment, the amount of the acid solution a is 7.5-8.5 times of the solid phase mass of the rice bran zymolyte;
in a specific embodiment, the acid solution a is an aqueous hydrochloric acid solution, an aqueous sulfuric acid solution, an aqueous phosphoric acid solution and/or an aqueous nitric acid solution;
in a specific embodiment, the acid treatment is carried out at 25-30 ℃;
in a particular embodiment, the acid treatment is carried out under stirring, sonication and/or shaker conditions;
in a specific embodiment, the acid treatment time is from 4 to 12 hours;
in a specific embodiment, the acid treatment time is from 5 to 8 hours;
in a specific embodiment, the method further comprises the step of filtering and collecting the filtrate to obtain the component A containing phytic acid.
In a particular embodiment, in said step a), the ion exchange resin is selected from a macroporous anion exchange resin and/or an anion exchange resin;
in a particular embodiment, the anion exchange resin is selected from a strong base anion exchange resin and/or a weak base anion exchange resin;
in a specific embodiment, the ion exchange resin B having adsorbed phytic acid is washed to neutrality.
In a specific embodiment, the anion exchange resin B having adsorbed phytic acid is washed to neutrality.
In a particular embodiment, in step B), the amount of phytase used is 0.1-1% by mass of the ion exchange resin B on which phytic acid is adsorbed;
in a particular embodiment, in step B), the amount of phytase used is 0.1-1% by mass of the anion exchange resin B on which phytic acid is adsorbed;
in a specific embodiment, the mass ratio of the resin to the phytase-containing aqueous solution is 1:1-1: 5;
in a specific embodiment, the mass ratio of the resin to the phytase-containing aqueous solution is 1:2-1: 3;
in a specific embodiment, the pH value of the enzyme solution is 3-6,
in a specific embodiment, the pH value of the enzyme solution is 4.5-5.5;
in a specific embodiment, the reaction temperature is from 40 ℃ to 60 ℃;
in a specific embodiment, the contacting means is a shaker reaction and/or a packed column reaction;
in a specific embodiment, the shaking table reaction speed is 50-200 rpm;
in a specific embodiment, the contact time is 4 to 24 hours;
in a particular embodiment, the liquid phase C is collected after the contacting.
In a particular embodiment, in said step d), the liquid phase C is concentrated to obtain inositol;
in a particular embodiment, the liquid phase C is concentrated to obtain crystals of inositol;
in a specific embodiment, inositol crystals are obtained by crystallization at 25-30 ℃ after concentration of the liquid phase C;
in a particular embodiment, after concentrating the liquid phase C, ethanol is added to obtain inositol crystals;
in a specific embodiment, absolute ethanol is used;
in a specific embodiment, 3 to 10 times the volume of absolute ethanol is added based on the volume of the concentrated liquid phase C;
in a particular embodiment, the inositol is greater than 98% pure.
In a second aspect, the present invention provides a method for preparing disodium hydrogen phosphate, comprising:
a) contacting an aqueous solution containing phytic acid with an ion exchange resin A to obtain an ion exchange resin B absorbed with phytic acid;
b) contacting the ion exchange resin B adsorbed with the phytic acid with phytase and water;
d) a step of obtaining a solid phase D; and
e) and (3) separating and obtaining the disodium hydrogen phosphate or hydrate thereof from the solid phase D.
In a particular embodiment, prior to step a) comprises:
a1) contacting rice bran with enzyme to obtain rice bran zymolyte; and
a2) treating the rice bran zymolyte, separating and obtaining the aqueous solution containing the phytic acid.
In a specific embodiment, in step a1), contacting rice bran with amylase and/or protease to obtain a rice bran hydrolysate;
in a particular embodiment, the amylase is selected from at least one of α amylase, β amylase, gamma amylase, and isoamylase;
in a specific embodiment, the protease is selected from at least one of an alkaline protease, an acidic protease and a neutral protease;
in a specific embodiment, the amylase and/or protease is/are used in an amount of 0.1-0.5% by mass of rice bran;
in a particular embodiment, the rice bran is defatted rice bran and/or non-defatted rice bran.
In a specific embodiment, further comprises the step of separating and removing the liquid phase of the rice bran hydrolysate;
in a specific embodiment, the rice bran hydrolysate liquid phase is removed by centrifugation;
in a specific embodiment, the rice bran hydrolysate liquid phase is removed by centrifugation to obtain a rice bran hydrolysate solid phase;
in a specific embodiment, the acid treatment of the rice bran enzymatic hydrolysate comprises suspending a rice bran enzymatic hydrolysate solid phase in water to obtain a rice bran enzymatic hydrolysate suspension, and adjusting the pH of the rice bran enzymatic hydrolysate suspension;
in a specific embodiment, the ratio of the solid phase of the rice bran hydrolysate to the water in the rice bran hydrolysate suspension is 1:6-1: 10;
in a specific embodiment, the ratio of the solid phase of the rice bran hydrolysate to the water in the rice bran hydrolysate suspension is 1:6-1: 8;
in a specific embodiment, the ratio of the solid phase of the rice bran hydrolysate to the water in the rice bran hydrolysate suspension is 1:7.5-1: 8.5;
in a specific embodiment, the adjusting the pH of the rice bran hydrolysate suspension is 1-3;
in a particular embodiment, the acid treatment of the rice bran enzymatic hydrolysate comprises contacting the rice bran enzymatic hydrolysate solid phase with an acid solution a;
in a specific embodiment, the pH of acid solution a is 1-3;
in a specific embodiment, the pH of acid solution a is 1.5-2.5;
in a specific embodiment, the amount of the acid solution a is 6-10 times of the solid phase mass of the rice bran zymolyte;
in a specific embodiment, the amount of the acid solution a is 6-8 times of the solid phase mass of the rice bran zymolyte;
in a specific embodiment, the amount of the acid solution a is 7.5-8.5 times of the solid phase mass of the rice bran zymolyte;
in a specific embodiment, the acid solution a is an aqueous hydrochloric acid solution, an aqueous sulfuric acid solution, an aqueous phosphoric acid solution and/or an aqueous nitric acid solution;
in a specific embodiment, the acid treatment is carried out at 25-30 ℃;
in a particular embodiment, the acid treatment is carried out under stirring, sonication and/or shaker conditions;
in a specific embodiment, the acid treatment time is from 4 to 12 hours;
in a specific embodiment, the acid treatment time is from 5 to 8 hours;
in a specific embodiment, the method further comprises the step of filtering and collecting the filtrate to obtain the component A containing phytic acid.
In a particular embodiment, in said step a), the ion exchange resin is selected from a macroporous anion exchange resin and/or an anion exchange resin;
in a particular embodiment, the anion exchange resin is selected from a strong base anion exchange resin and/or a weak base anion exchange resin;
in a specific embodiment, the ion exchange resin B having adsorbed phytic acid is washed to neutrality.
In a specific embodiment, the anion exchange resin B having adsorbed phytic acid is washed to neutrality.
In a particular embodiment, in step B), the amount of phytase used is 0.1-1% by mass of the ion exchange resin B on which phytic acid is adsorbed;
in a particular embodiment, in step B), the amount of phytase used is 0.1-1% by mass of the anion exchange resin B on which phytic acid is adsorbed;
in a specific embodiment, the mass ratio of the resin to the phytase-containing aqueous solution is 1:1-1: 5;
in a specific embodiment, the mass ratio of the resin to the phytase-containing aqueous solution is 1:2-1: 3;
in a specific embodiment, the pH value of the enzyme solution is 3-6,
in a specific embodiment, the pH value of the enzyme solution is 4.5-5.5;
in a specific embodiment, the reaction temperature is from 40 ℃ to 60 ℃;
in a specific embodiment, the contacting means is a shaker reaction and/or a packed column reaction;
in a specific embodiment, the shaking table reaction speed is 50-200 rpm;
in a specific embodiment, the contact time is 4 to 24 hours;
in a specific embodiment, the solid phase D is collected after the contacting.
In a particular embodiment, in said step f), the solid phase D is eluted with a lye to obtain a disodium hydrogen phosphate solution;
in a specific embodiment, the alkali liquor is at least one selected from NaOH, KOH, Na2CO3, K2CO 3;
in a specific embodiment, the mass concentration of the alkali liquor is 1-10%;
in a specific embodiment, the alkali liquor is NaOH solution with the mass concentration of 1-5%;
in a specific embodiment, the disodium phosphate solution is further concentrated;
in a particular embodiment, further crystallization yields disodium hydrogen phosphate hydrate;
in a specific embodiment, 3 to 10 times by volume of anhydrous ethanol is further added to the concentrated disodium hydrogen phosphate solution based on the volume of the concentrated disodium hydrogen phosphate solution, and crystallization is performed to obtain disodium hydrogen phosphate hydrate;
in a specific embodiment, the disodium phosphate hydrate has a degree of hydration of 1 to 12;
in a particular embodiment, the disodium phosphate hydrate is greater than 98% pure.
In a specific embodiment, the method further comprises dehydrating the disodium hydrogen phosphate hydrate.
In a specific embodiment, the dehydration treatment process employs a heat treatment;
in a specific embodiment, the dehydration treatment process is performed under vacuum conditions.
In a third aspect, the present invention also provides the inositol prepared by the process described in the first aspect above.
In a particular embodiment, the inositol is greater than 98% pure.
In a fourth aspect, the present invention also provides disodium hydrogen phosphate or a hydrate thereof prepared by the method described in the second aspect above.
In a particular embodiment, the disodium phosphate or hydrate thereof is more than 98% pure.
In a fifth aspect, the present invention provides a process for preparing inositol from rice bran, comprising:
a1) contacting rice bran with enzyme to obtain rice bran zymolyte;
a2) treating the rice bran zymolyte, separating and obtaining the aqueous solution containing the phytic acid.
a) Contacting an aqueous solution containing phytic acid with an ion exchange resin A to obtain an ion exchange resin B absorbed with phytic acid;
b) contacting the ion exchange resin B adsorbed with the phytic acid with phytase and water to obtain a liquid phase C; and
c) separating the inositol from the liquid phase C.
In a specific embodiment, in step a1), contacting rice bran with amylase and/or protease to obtain a rice bran hydrolysate;
in a particular embodiment, the amylase is selected from at least one of α amylase, β amylase, gamma amylase, and isoamylase;
in a specific embodiment, the protease is selected from at least one of an alkaline protease, an acidic protease and a neutral protease;
in a specific embodiment, the amylase and/or protease is/are used in an amount of 0.1-0.5% by mass of rice bran;
in a particular embodiment, the rice bran is defatted rice bran and/or non-defatted rice bran.
In a specific embodiment, further comprises the step of separating and removing the liquid phase of the rice bran hydrolysate;
in a specific embodiment, the rice bran hydrolysate liquid phase is removed by centrifugation;
in a specific embodiment, the acid treatment of the rice bran enzymatic hydrolysate comprises suspending a rice bran enzymatic hydrolysate solid phase in water to obtain a rice bran enzymatic hydrolysate suspension, and adjusting the pH of the rice bran enzymatic hydrolysate suspension;
in a specific embodiment, the ratio of the solid phase of the rice bran hydrolysate to the water in the rice bran hydrolysate suspension is 1:6-1: 10;
in a specific embodiment, the ratio of the solid phase of the rice bran hydrolysate to the water in the rice bran hydrolysate suspension is 1:6-1: 8;
in a specific embodiment, the ratio of the solid phase of the rice bran hydrolysate to the water in the rice bran hydrolysate suspension is 1:7.5-1: 8.5;
in a specific embodiment, the adjusting the pH of the rice bran hydrolysate suspension is 1-3;
in a particular embodiment, the acid treatment of the rice bran enzymatic hydrolysate comprises contacting the rice bran enzymatic hydrolysate solid phase with an acid solution a;
in a specific embodiment, the pH of acid solution a is 1-3;
in a specific embodiment, the pH of acid solution a is 1.5-2.5;
in a specific embodiment, the amount of the acid solution a is 6-10 times of the mass of the rice bran enzymolysis solid phase substance;
in a specific embodiment, the amount of the acid solution a is 6-8 times of the solid phase mass of the rice bran zymolyte;
in a specific embodiment, the amount of the acid solution a is 7.5-8.5 times of the solid phase mass of the rice bran zymolyte;
in a specific embodiment, the acid solution a is an aqueous hydrochloric acid solution, an aqueous sulfuric acid solution, an aqueous phosphoric acid solution and/or an aqueous nitric acid solution;
in a specific embodiment, the acid treatment is carried out at 25-30 ℃;
in a particular embodiment, the acid treatment is carried out under stirring, sonication and/or shaker conditions;
in a specific embodiment, the acid treatment time is from 4 to 12 hours;
in a specific embodiment, the acid treatment time is from 5 to 8 hours;
in a specific embodiment, the method further comprises the step of filtering and collecting the filtrate to obtain the component A containing phytic acid.
In a particular embodiment, in said step a), the ion exchange resin is selected from a macroporous anion exchange resin and/or an anion exchange resin;
in a particular embodiment, the anion exchange resin is selected from a strong base anion exchange resin and/or a weak base anion exchange resin;
in a specific embodiment, the ion exchange resin B having adsorbed phytic acid is washed to neutrality.
In a specific embodiment, the anion exchange resin B having adsorbed phytic acid is washed to neutrality.
In a particular embodiment, in step B), the amount of phytase used is 0.1-1% by mass of the ion exchange resin B on which phytic acid is adsorbed;
in a particular embodiment, in step B), the amount of phytase used is 0.1-1% by mass of the anion exchange resin B on which phytic acid is adsorbed;
in a specific embodiment, the mass ratio of the resin to the phytase-containing aqueous solution is 1:1-1: 5;
in a specific embodiment, the mass ratio of the resin to the phytase-containing aqueous solution is 1:2-1: 3;
in a specific embodiment, the pH value of the enzyme solution is 3-6,
in a specific embodiment, the pH value of the enzyme solution is 4.5-5.5;
in a specific embodiment, the reaction temperature is from 40 ℃ to 60 ℃;
in a specific embodiment, the contacting means is a shaker reaction and/or a packed column reaction;
in a specific embodiment, the shaking table reaction speed is 50-200 rpm;
in a specific embodiment, the contact time is 4 to 24 hours;
in a particular embodiment, the liquid phase C is collected after the contacting.
In a particular embodiment, in said step d), the liquid phase C is concentrated to obtain inositol;
in a particular embodiment, the liquid phase C is concentrated to obtain crystals of inositol;
in a specific embodiment, inositol crystals are obtained by crystallization at 25-30 ℃ after concentration of the liquid phase C;
in a particular embodiment, after concentrating the liquid phase C, ethanol is added to obtain inositol crystals;
in a specific embodiment, absolute ethanol is used;
in a specific embodiment, 3 to 10 times the volume of absolute ethanol is added based on the volume of the concentrated liquid phase C;
in a particular embodiment, the inositol is greater than 98% pure.
In a sixth aspect, the present invention provides a process for preparing disodium hydrogen phosphate from rice bran, comprising:
a1) contacting rice bran with enzyme to obtain rice bran zymolyte;
a2) processing the rice bran zymolyte, separating and obtaining an aqueous solution containing phytic acid;
a) contacting an aqueous solution containing phytic acid with an ion exchange resin A to obtain an ion exchange resin B absorbed with phytic acid;
b) contacting the ion exchange resin B adsorbed with the phytic acid with phytase and water;
d) a step of obtaining a solid phase D; and
e) and (3) separating and obtaining the disodium hydrogen phosphate or hydrate thereof from the solid phase D.
In a specific embodiment, in step a1), contacting rice bran with amylase and/or protease to obtain a rice bran hydrolysate;
in a particular embodiment, the amylase is selected from at least one of α amylase, β amylase, gamma amylase, and isoamylase;
in a specific embodiment, the protease is selected from at least one of an alkaline protease, an acidic protease and a neutral protease;
in a specific embodiment, the amylase and/or protease is/are used in an amount of 0.1-0.5% by mass of rice bran;
in a particular embodiment, the rice bran is defatted rice bran and/or non-defatted rice bran.
In a specific embodiment, further comprises the step of separating and removing the liquid phase of the rice bran hydrolysate;
in a specific embodiment, the rice bran hydrolysate liquid phase is removed by centrifugation;
in a specific embodiment, the rice bran hydrolysate liquid phase is removed by centrifugation to obtain a rice bran hydrolysate solid phase;
in a specific embodiment, the acid treatment of the rice bran enzymatic hydrolysate comprises suspending a rice bran enzymatic hydrolysate solid phase in water to obtain a rice bran enzymatic hydrolysate suspension, and adjusting the pH of the rice bran enzymatic hydrolysate suspension;
in a specific embodiment, the ratio of the solid phase of the rice bran hydrolysate to the water in the rice bran hydrolysate suspension is 1:6-1: 10;
in a specific embodiment, the ratio of the solid phase of the rice bran hydrolysate to the water in the rice bran hydrolysate suspension is 1:6-1: 8;
in a specific embodiment, the ratio of the solid phase of the rice bran hydrolysate to the water in the rice bran hydrolysate suspension is 1:7.5-1: 8.5;
in a specific embodiment, the adjusting the pH of the rice bran hydrolysate suspension is 1-3;
in a particular embodiment, the acid treatment of the rice bran enzymatic hydrolysate comprises contacting the rice bran enzymatic hydrolysate solid phase with an acid solution a;
in a specific embodiment, the pH of acid solution a is 1-3;
in a specific embodiment, the pH of acid solution a is 1.5-2.5;
in a specific embodiment, the amount of the acid solution a is 6-10 times of the solid phase mass of the rice bran zymolyte;
in a specific embodiment, the amount of the acid solution a is 6-8 times of the solid phase mass of the rice bran zymolyte;
in a specific embodiment, the amount of the acid solution a is 7.5-8.5 times of the solid phase mass of the rice bran zymolyte;
in a specific embodiment, the acid solution a is an aqueous hydrochloric acid solution, an aqueous sulfuric acid solution, an aqueous phosphoric acid solution and/or an aqueous nitric acid solution;
in a specific embodiment, the acid treatment is carried out at 25-30 ℃;
in a particular embodiment, the acid treatment is carried out under stirring, sonication and/or shaker conditions;
in a specific embodiment, the acid treatment time is from 4 to 12 hours;
in a specific embodiment, the acid treatment time is from 5 to 8 hours;
in a specific embodiment, the method further comprises the step of filtering and collecting the filtrate to obtain the component A containing phytic acid.
In a particular embodiment, in said step a), the ion exchange resin is selected from a macroporous anion exchange resin and/or an anion exchange resin;
in a particular embodiment, the anion exchange resin is selected from a strong base anion exchange resin and/or a weak base anion exchange resin;
in a specific embodiment, the ion exchange resin B having adsorbed phytic acid is washed to neutrality.
In a specific embodiment, the anion exchange resin B having adsorbed phytic acid is washed to neutrality.
In a particular embodiment, in step B), the amount of phytase used is 0.1-1% by mass of the ion exchange resin B on which phytic acid is adsorbed;
in a particular embodiment, in step B), the amount of phytase used is 0.1-1% by mass of the anion exchange resin B on which phytic acid is adsorbed;
in a specific embodiment, the mass ratio of the resin to the phytase-containing aqueous solution is 1:1-1: 5;
in a specific embodiment, the mass ratio of the resin to the phytase-containing aqueous solution is 1:2-1: 3;
in a specific embodiment, the pH value of the enzyme solution is 3-6,
in a specific embodiment, the pH value of the enzyme solution is 4.5-5.5;
in a specific embodiment, the reaction temperature is from 40 ℃ to 60 ℃;
in a specific embodiment, the contacting means is a shaker reaction and/or a packed column reaction;
in a specific embodiment, the shaking table reaction speed is 50-200 rpm;
in a specific embodiment, the contact time is 4 to 24 hours;
in a specific embodiment, the solid phase D is collected after the contacting.
In a particular embodiment, in said step f), the solid phase D is eluted with a lye to obtain a disodium hydrogen phosphate solution;
in a specific embodiment, the alkali liquor is at least one selected from NaOH, KOH, Na2CO3, K2CO 3;
in a specific embodiment, the mass concentration of the alkali liquor is 1-10%;
in a specific embodiment, the alkali liquor is NaOH solution with the mass concentration of 1-5%;
in a specific embodiment, the disodium phosphate solution is further concentrated;
in a particular embodiment, further crystallization yields disodium hydrogen phosphate hydrate;
in a specific embodiment, 3 to 10 times by volume of anhydrous ethanol is further added to the concentrated disodium hydrogen phosphate solution based on the volume of the concentrated disodium hydrogen phosphate solution, and crystallization is performed to obtain disodium hydrogen phosphate hydrate;
in a specific embodiment, the disodium phosphate hydrate has a degree of hydration of 1 to 12;
in a particular embodiment, the disodium phosphate hydrate is greater than 98% pure.
In a specific embodiment, the method further comprises dehydrating the disodium hydrogen phosphate hydrate.
In a specific embodiment, the dehydration treatment process employs a heat treatment;
in a specific embodiment, the dehydration treatment process is performed under vacuum conditions.
The method extracts inositol from the filter residue generated in the process of preparing the water-soluble nutrient powder from the rice bran and prepares the disodium hydrogen phosphate, so that the rice bran is fully utilized, the process for extracting the inositol is simple, the disodium hydrogen phosphate is prepared by utilizing phosphate ions generated in enzymatic hydrolysis, the product is easy to separate and purify, and the purity of the obtained inositol and the disodium hydrogen phosphate is higher than 98 percent.
Detailed Description
The embodiments and technical effects of the present invention will be specifically described below with reference to examples, but the present invention is not limited to the following examples.
Raw materials:
defatted testa oryzae and fresh testa oryzae (non-defatted testa oryzae) source (Yihaijiali group)
Amylase (AMYLEX LT, Denisc China Co., Ltd.)
Protease (Alcalase 2.4L, Novoxin Biotechnology Co., Ltd.)
Phytase (Phytase 5000L, Disman China Co., Ltd.)
Macroporous anion exchange resin (Seplite LX-300C, Xian lan Xiao science and technology New materials Co., Ltd.)
Cation exchange resin (NKA-9, Nankai resin Co., Ltd.)
Adsorbent resin (LSA-40, Xian lan Xiao science and technology New materials GmbH)
NaOH (national drug group chemical agent Co., Ltd.)
Absolute ethyl alcohol (national medicine group chemical reagent Co., Ltd.)
1 determination of inositol content:
directly dissolving a sample, filtering, separating by an ion chromatography Carb1 sugar column, detecting by a pulse ampere detector, quantifying by an external standard method, wherein the mobile phase is (5mmol NaOH +2mmol AcNa)/L, the detector mode PAD is adopted, the flow rate is 1mL/min, the column temperature is not controlled, the sample feeding amount is 20 mu L, and the chromatographic column is Carb-1150 mm × 4 mm.
2 determination of the content of disodium hydrogen phosphate
Accurately adding an excessive hydrochloric acid standard titration solution into a sample, titrating the excessive hydrochloric acid standard titration solution by using a sodium hydroxide standard titration solution, indicating a jump point by using an acidimeter, and calculating the content of the disodium hydrogen phosphate according to the consumption of the sodium hydroxide standard titration solution.
3 preparation of insoluble components of rice bran:
3.1 weighing 1000g of non-defatted rice bran, dispersing the non-defatted rice bran in 6000g of water, adding 0.3% of amylase, reacting for 1h at 50 ℃, then adjusting the pH value to 8-9, adding 0.5% of protease, continuing to react for 2h, filtering to obtain a rice bran insoluble component I, and placing the rice bran insoluble component I in a refrigerator for later use.
3.2 weighing 1000g defatted rice bran, dispersing in 6000g water, stirring at 50 deg.C for 1h, filtering to obtain rice bran insoluble component II, and standing in refrigerator for use.
3.3 weighing 1000g of non-defatted rice bran, dispersing in 6000g of water, adding 0.3% amylase, reacting at 50 deg.C for 1h, filtering to obtain rice bran insoluble component III, and standing in refrigerator for use.
3.4 weighing 1000g of defatted rice bran, dispersing in 6000g of water, adjusting the pH value to 8-9, adding 0.5% protease, reacting for 2h, filtering to obtain an insoluble component IV of the rice bran, and placing in a refrigerator for later use.
4 examples
The preparation method of the phytic acid aqueous solution comprises the following steps:
example 1
Weighing 50g rice bran insoluble component I, adding 300g water, adjusting pH to 2, stirring at room temperature for 4h, filtering, and collecting filtrate.
Example 2
Weighing 50g rice bran insoluble component I, adding 400g water, adjusting pH to 1, stirring at room temperature for 6h, filtering, and collecting filtrate.
Example 3
Weighing 50g rice bran insoluble fraction I, adding 500g water, adjusting pH to 3, stirring at room temperature for 12h, filtering, and collecting filtrate.
Example 4
Weighing 50g rice bran insoluble component I, adding 300g water, adjusting pH to 2, stirring at room temperature for 4h, filtering, and collecting filtrate.
Example 5
Weighing 50g rice bran insoluble component I, adding 300g water, adjusting pH to 2, stirring at room temperature for 4h, filtering, and collecting filtrate.
Example 6
Weighing 50g rice bran insoluble component II, adding 300g water, adjusting pH to 3, stirring at room temperature for 4h, filtering, and collecting filtrate.
Example 7
Weighing 50g rice bran insoluble component III, adding 300g water, adjusting pH to 3, stirring at room temperature for 4 hr, filtering, and collecting filtrate.
Example 8
Weighing 50g rice bran insoluble component IV, adding 300g water, adjusting pH to 3, stirring at room temperature for 4 hr, filtering, and collecting filtrate.
The preparation method of the inositol comprises the following steps:
example 1a
The phytic acid-containing aqueous solution obtained in example 1 was subjected to ion exchange through a column packed with 30g of macroporous anion exchange resin, then the resin was washed with water to neutrality, charged into a 100mL Erlenmeyer flask, added with 30mL of water to adjust the pH to 5, and then added with 0.5% phytase, the enzyme activity was 5000U/g, and reacted for 6 hours in a shaker (rotation speed 150rpm) at 50 ℃. After the reaction, the filtrate was collected by filtration, boiled for 10 minutes, concentrated and crystallized at room temperature to precipitate inositol 1.2g with a purity of 99%.
Example 2a
The phytic acid-containing aqueous solution obtained in example 2 was subjected to ion exchange through a column packed with 30g of macroporous anion exchange resin, then the resin was washed with water to neutrality, charged into a 100mL Erlenmeyer flask, added with 60mL of water to adjust the pH to 4.5, and then added with 0.1% phytase, the enzyme activity was 5000U/g, and reacted for 4 hours in a shaker (rotation speed 100rpm) at 55 ℃. After the reaction is finished, filtering and collecting filtrate, boiling the filtrate for 10 minutes, concentrating, crystallizing at room temperature to separate out 0.9g of inositol with the purity of 99%.
Example 3a
The phytic acid-containing aqueous solution obtained in example 3 was subjected to ion exchange through a column packed with 30g of macroporous anion exchange resin, then the resin was washed with water to neutrality, charged into a 250mL Erlenmeyer flask, added with 150mL of water to adjust the pH to 3.5, and then added with 0.5% phytase, the enzyme activity was 5000U/g, and reacted for 8 hours in a shaker (rotation speed 150rpm) at 45 ℃. After the reaction, the filtrate was collected by filtration, boiled for 10 minutes, concentrated and crystallized at room temperature to precipitate 1.1g of inositol with a purity of 98.5%.
Example 4a
The phytic acid-containing aqueous solution obtained in example 4 was subjected to ion exchange through a column packed with 30g of macroporous anion exchange resin, then the resin was washed with water to neutrality, charged into a 250mL Erlenmeyer flask, added with 90mL of water to adjust the pH to 5.5, and then added with 0.3% phytase, the enzyme activity was 5000U/g, and reacted in a shaker (rotation speed 200rpm) at 60 ℃ for 16 hours. After the reaction, the filtrate was collected by filtration, boiled for 10 minutes, concentrated and crystallized at room temperature to precipitate inositol 0.8g with a purity of 98.5%.
Example 5a
The phytic acid-containing aqueous solution obtained in example 5 was subjected to ion exchange through a column packed with 30g of macroporous anion exchange resin, then the resin was washed with water to neutrality, charged into a 100mL Erlenmeyer flask, added with 60mL of water to adjust the pH to 4.5, and then added with 0.2% phytase, the enzyme activity was 5000U/g, and reacted in a shaker (rotation speed 50rpm) at 50 ℃ for 24 hours. After the reaction, the filtrate was collected by filtration, boiled for 10 minutes, concentrated and crystallized at room temperature to precipitate inositol 1.0g with a purity of 99%.
Example 6a
The phytic acid-containing aqueous solution obtained in example 6 was subjected to ion exchange through a column packed with 30g of macroporous anion exchange resin, then the resin was washed with water to neutrality, charged into a 100mL Erlenmeyer flask, added with 60mL of water to adjust the pH to 4.5, and then added with 0.2% phytase, the enzyme activity was 5000U/g, and reacted in a shaker (rotation speed 50rpm) at 50 ℃ for 24 hours. After the reaction is finished, filtering and collecting filtrate, boiling the filtrate for 10 minutes, concentrating, crystallizing at room temperature to separate out 0.4g of inositol with the purity of 90%.
Example 7a
The phytic acid-containing aqueous solution obtained in example 7 was subjected to ion exchange through a column packed with 30g of macroporous anion exchange resin, then the resin was washed with water to neutrality, charged into a 100mL Erlenmeyer flask, added with 60mL of water to adjust the pH to 4.5, and then added with 0.2% phytase, the enzyme activity was 5000U/g, and reacted in a shaker (rotation speed 50rpm) at 50 ℃ for 24 hours. After the reaction, the filtrate was collected by filtration, boiled for 10 minutes, concentrated and crystallized at room temperature to precipitate inositol 0.6g with a purity of 95%.
Example 8a
The phytic acid-containing aqueous solution obtained in example 8 was subjected to ion exchange through a column packed with 30g of macroporous anion exchange resin, then the resin was washed with water to neutrality, charged into a 100mL Erlenmeyer flask, added with 60mL of water to adjust the pH to 4.5, and then added with 0.2% phytase, the enzyme activity was 5000U/g, and reacted in a shaker (rotation speed 50rpm) at 50 ℃ for 24 hours. After the reaction, the filtrate was collected by filtration, boiled for 10 minutes, concentrated and crystallized at room temperature to precipitate inositol 0.6g with a purity of 92%.
Example 9a
The phytic acid-containing aqueous solution obtained in example 1 was subjected to ion exchange through a column filled with 30g of cation exchange resin, then the resin was washed with water to neutrality, charged into a 100mL Erlenmeyer flask, added with 60mL of water to adjust the pH to 4.5, and then added with 0.2% phytase having an enzyme activity of 5000U/g, and reacted in a shaker (rotation speed 50rpm) at 50 ℃ for 24 hours. After the reaction is finished, filtering and collecting filtrate, boiling the filtrate for 10 minutes, and concentrating the filtrate to ensure that inositol is not separated out at room temperature.
Example 10a
The phytic acid-containing aqueous solution obtained in example 1 was subjected to ion exchange through a column filled with 30g of an adsorbent resin, then the resin was washed with water to neutrality, charged into a 100mL Erlenmeyer flask, added with 60mL of water, adjusted to pH 4.5, and then added with 0.2% phytase having an enzyme activity of 5000U/g, and reacted in a shaker (rotation speed 50rpm) at 50 ℃ for 24 hours. After the reaction is finished, filtering and collecting filtrate, boiling the filtrate for 10 minutes, and concentrating the filtrate to ensure that inositol is not separated out at room temperature.
Example 11a
The phytic acid-containing aqueous solution obtained in example 2 was subjected to ion exchange through a column packed with 30g of macroporous anion exchange resin, then the resin was washed with water to neutrality, charged into a 100mL Erlenmeyer flask, added with 60mL of water to adjust the pH to 4.5, and then added with 0.1% phytase, the enzyme activity was 5000U/g, and reacted for 4 hours in a shaker (rotation speed 100rpm) at 55 ℃. After the reaction is finished, filtering and collecting filtrate, boiling the filtrate for 10 minutes, concentrating, and adding 10mL of absolute ethyl alcohol to separate out 0.9g of inositol with the purity of 99%.
The preparation method of the disodium hydrogen phosphate comprises the following steps:
example 1b
The resin from example 1a was washed with 1% NaOH solution, the filtrate was concentrated and 5.6g of disodium hydrogenphosphate hydrate crystallized at room temperature to give 98.5% purity.
Example 2b
The resin from example 2a was washed with 3% NaOH solution, the filtrate was concentrated and 5.1g of disodium hydrogenphosphate hydrate crystallized at room temperature to give 99% purity.
Example 3b
The resin from example 3a was washed with 2% NaOH solution, the filtrate was concentrated and 5.2g of disodium hydrogenphosphate hydrate crystallized at room temperature to give 99% purity.
Example 4b
The resin from example 4a was washed with 5% NaOH solution, the filtrate was concentrated and 4.9g of disodium hydrogenphosphate hydrate crystallized at room temperature to give 98.5% purity.
Example 5b
The resin from example 5a was washed with 2% NaOH solution, the filtrate was concentrated and 5.2g of disodium hydrogenphosphate hydrate crystallized at room temperature to give 98.5% purity.
Example 6b
The resin from example 5a was washed with 2% NaOH solution, the filtrate was concentrated and 15mL of absolute ethanol was added to precipitate 5.2g of disodium hydrogen phosphate hydrate with a purity of 98.5%.
Claims (10)
1. A process for producing inositol, comprising:
a) contacting an aqueous solution containing phytic acid with an ion exchange resin A to obtain an ion exchange resin B absorbed with phytic acid;
b) contacting the ion exchange resin B adsorbed with the phytic acid with phytase and water;
c) a step of obtaining a liquid phase C; and
d) separating the inositol from the liquid phase C.
2. A method for preparing disodium hydrogen phosphate, which is characterized by comprising the following steps:
a) contacting an aqueous solution containing phytic acid with an ion exchange resin A to obtain an ion exchange resin B absorbed with phytic acid;
b) contacting the ion exchange resin B adsorbed with the phytic acid with phytase and water;
e) a step of obtaining a solid phase D; and
f) and (3) separating and obtaining the disodium hydrogen phosphate or hydrate thereof from the solid phase D.
3. The method of claim 1 or 2, comprising, prior to step a):
a1) contacting rice bran with enzyme to obtain rice bran zymolyte; and
a2) treating the rice bran zymolyte, separating and obtaining the aqueous solution containing the phytic acid.
4. A process according to claim 3, wherein in step a1), the rice bran is contacted with amylase and/or protease to obtain a rice bran hydrolysate;
preferably, the amylase is selected from at least one of α amylase, β amylase, gamma amylase and isoamylase, preferably, the protease is selected from at least one of alkaline protease, acid protease and neutral protease;
preferably, the dosage of the amylase and/or the protease is 0.1-0.5% of the mass of rice bran; or
Preferably, the rice bran is defatted rice bran and/or non-defatted rice bran.
5. The method as claimed in claim 3, wherein the step a2) comprises the steps of acid-treating the rice bran zymolyte, separating to obtain an aqueous solution containing phytic acid;
preferably, the method further comprises the step of separating and removing the liquid phase of the rice bran enzymatic hydrolysate to obtain a solid phase of the rice bran enzymatic hydrolysate, and preferably removing the liquid phase of the rice bran enzymatic hydrolysate by centrifugation;
preferably, the acid-treating the rice bran enzymatic hydrolysate comprises suspending the rice bran enzymatic hydrolysate obtained in the separation step a1) in a solid phase in water to obtain a rice bran enzymatic hydrolysate suspension, and adjusting the pH of the rice bran enzymatic hydrolysate suspension;
preferably, in the rice bran zymolyte suspension, the ratio of the solid phase of the rice bran zymolyte to water is 1:6-1: 10;
preferably, the pH value of the rice bran zymolyte suspension is adjusted to be 1-3; preferably, the acid treatment of the rice bran zymolyte comprises contacting the rice bran zymolyte solid phase with an acid solution a;
preferably, the pH of the acid solution a is 1-3;
preferably, the amount of the acid solution a is 6-10 times of the solid phase mass of the rice bran zymolyte;
preferably, the acid solution a is a hydrochloric acid aqueous solution, a sulfuric acid aqueous solution, a phosphoric acid aqueous solution and/or a nitric acid aqueous solution; preferably, the acid treatment is carried out at 25-30 ℃;
preferably, the acid treatment is carried out under stirring, sonication and/or shaker conditions;
preferably, the acid treatment time is 4 to 12 hours; or
Preferably, the method further comprises a step of filtering operation, and collecting the filtrate to obtain the component A containing the phytic acid.
6. The process according to claim 1 or 2, wherein in step a) the ion exchange resin is selected from a macroporous anion exchange resin and/or an anion exchange resin,
strong base anion exchange resins and/or weak base anion exchange resins are preferred; or
Preferably, the ion exchange resin B absorbed with phytic acid is washed to be neutral.
7. The method according to claim 1 or 2, wherein in step B), the amount of phytase used is 0.1-1% by mass of the ion exchange resin B on which phytic acid is adsorbed;
preferably, the mass ratio of the resin to the phytase-containing aqueous solution is 1:1-1:5, or 1:2-1: 3;
preferably, the pH value of the enzyme solution is 3-6, or 4.5-5.5; preferably, the reaction temperature is 40-60 ℃;
preferably, the contact mode is a shaking table reaction and/or a packed column reaction;
preferably, the reaction speed of the shaking table is 50-200 rpm; or
Preferably, the contact time is 4-24 h.
8. The process of claim 1, wherein in step d), the liquid phase C is concentrated to obtain inositol;
preferably, the liquid phase C is concentrated to obtain inositol crystals;
preferably, the inositol crystal is obtained by crystallization at 25-30 ℃ after the liquid phase C is concentrated;
preferably, after concentrating the liquid phase C, adding ethanol to obtain inositol crystals, preferably using anhydrous ethanol;
preferably, 3-10 times of volume of absolute ethyl alcohol is added according to the volume of the concentrated liquid phase C; or
Preferably, the inositol is greater than 98% pure.
9. The method of claim 2, wherein in step f), the solid phase D is eluted with a lye to obtain a disodium hydrogen phosphate solution;
preferably, the alkali liquor is at least one selected from NaOH, KOH, Na2CO3 and K2CO 3;
preferably, the mass concentration of the alkali liquor is 1-10%;
preferably, the alkali liquor is NaOH solution with the mass concentration of 1-5%;
preferably, the disodium hydrogen phosphate solution is further concentrated; preferably, further crystallization yields disodium hydrogen phosphate hydrate;
preferably, 3-10 times of volume of absolute ethanol is added into the concentrated disodium hydrogen phosphate solution according to the volume of the concentrated disodium hydrogen phosphate solution, and disodium hydrogen phosphate hydrate is obtained through crystallization;
preferably, the hydration degree of the disodium hydrogen phosphate hydrate is 1-12; or
Preferably, the disodium hydrogen phosphate hydrate has a purity of greater than 98%.
10. Inositol produced by the process of any one of claims 1, 3-8 or disodium hydrogen phosphate hydrate produced by the process of any one of claims 2-9.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE102020200670A1 (en) | 2020-01-21 | 2021-07-22 | Rheinisch-Westfälische Technische Hochschule (Rwth) Aachen | Process for providing phosphate from a phytate-containing biomass, phytate- and phosphate-reduced biomass and uses thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH1075795A (en) * | 1996-09-04 | 1998-03-24 | Oji Koonsutaac Kk | Production of inositol |
| CN108101744A (en) * | 2017-12-14 | 2018-06-01 | 姜均 | Inositol extraction method |
-
2018
- 2018-12-29 CN CN201811637541.XA patent/CN111378692A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH1075795A (en) * | 1996-09-04 | 1998-03-24 | Oji Koonsutaac Kk | Production of inositol |
| CN108101744A (en) * | 2017-12-14 | 2018-06-01 | 姜均 | Inositol extraction method |
Non-Patent Citations (4)
| Title |
|---|
| 戴传波: "由玉米浸渍水制取肌醇的方法研究", 《中国优秀博硕士学位论文全文数据库(博士)工程科技Ⅰ辑》, no. 2005, pages 21 - 28 * |
| 李兴: "脱脂米糠中含植酸颗粒的提取及其成分分析和结构鉴定", 《中国优秀硕士学位论文全文数据库 工程科技Ⅰ辑》, no. 2014, pages 20 * |
| 田庆国: "米糠中提取肌醇的研究", 《食品工业》, no. 03, pages 17 - 18 * |
| 田玲玲: "米糠中肌醇的超声协同酶法制取及其超声结晶", 《中国优秀硕士学位论文全文数据库 工程科技Ⅰ辑》, no. 2016, pages 13 - 14 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE102020200670A1 (en) | 2020-01-21 | 2021-07-22 | Rheinisch-Westfälische Technische Hochschule (Rwth) Aachen | Process for providing phosphate from a phytate-containing biomass, phytate- and phosphate-reduced biomass and uses thereof |
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