CN111366674A - Multi-oil-cylinder pressurizing device for OPLC (optical phase separation liquid chromatography) pressurized thin-layer chromatograph - Google Patents
Multi-oil-cylinder pressurizing device for OPLC (optical phase separation liquid chromatography) pressurized thin-layer chromatograph Download PDFInfo
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- CN111366674A CN111366674A CN202010285908.7A CN202010285908A CN111366674A CN 111366674 A CN111366674 A CN 111366674A CN 202010285908 A CN202010285908 A CN 202010285908A CN 111366674 A CN111366674 A CN 111366674A
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- 230000003287 optical effect Effects 0.000 title abstract description 7
- 238000005191 phase separation Methods 0.000 title abstract description 5
- 238000004811 liquid chromatography Methods 0.000 title description 6
- 239000010720 hydraulic oil Substances 0.000 claims abstract description 26
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 14
- 239000003921 oil Substances 0.000 claims abstract description 7
- 239000010409 thin film Substances 0.000 claims description 11
- 229910000831 Steel Inorganic materials 0.000 claims description 10
- 239000010959 steel Substances 0.000 claims description 10
- 238000007789 sealing Methods 0.000 claims description 8
- -1 polytetrafluoroethylene Polymers 0.000 claims description 6
- 229920001343 polytetrafluoroethylene Polymers 0.000 claims description 6
- 239000004810 polytetrafluoroethylene Substances 0.000 claims description 6
- 238000001802 infusion Methods 0.000 claims description 5
- 239000002861 polymer material Substances 0.000 claims description 4
- 238000004519 manufacturing process Methods 0.000 claims description 3
- 239000000463 material Substances 0.000 claims description 3
- 230000000149 penetrating effect Effects 0.000 claims description 3
- 238000000926 separation method Methods 0.000 abstract description 4
- 239000007788 liquid Substances 0.000 abstract description 3
- 230000000694 effects Effects 0.000 abstract description 2
- 239000012530 fluid Substances 0.000 abstract description 2
- 239000010408 film Substances 0.000 description 12
- 238000004809 thin layer chromatography Methods 0.000 description 7
- 239000003463 adsorbent Substances 0.000 description 4
- 238000004458 analytical method Methods 0.000 description 2
- 238000004587 chromatography analysis Methods 0.000 description 2
- 238000013461 design Methods 0.000 description 2
- 238000009792 diffusion process Methods 0.000 description 2
- 239000002985 plastic film Substances 0.000 description 2
- 229920006255 plastic film Polymers 0.000 description 2
- 239000002904 solvent Substances 0.000 description 2
- 230000009471 action Effects 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000013375 chromatographic separation Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 231100000167 toxic agent Toxicity 0.000 description 1
- 239000003440 toxic substance Substances 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/90—Plate chromatography, e.g. thin layer or paper chromatography
- G01N30/94—Development
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- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Treatment Of Liquids With Adsorbents In General (AREA)
Abstract
The invention discloses a multi-cylinder pressurizing device of an OPLC (optical phase separation liquid) pressurizing thin-layer chromatograph, which comprises a working panel, a flat column clamping plate and a flat column, wherein the flat column clamping plate is arranged at the top of the working panel. When the expansion chamber, the flat column plate clamp and the flat column are pressurized, the stress center is two points (or four points) distributed at the center of the oil cylinder, and the deformation is obviously reduced compared with the deformation when one oil cylinder is designed originally, so that the uniformity of the pressure distribution of the expanding agent is facilitated, the tested sample is ensured to be linearly expanded along the specified direction, or a plurality of tested samples are linearly expanded in parallel, and a better component separation effect is obtained; when the instrument works, the oil pump connected with the hydraulic oil cylinder is started, the hydraulic oil is pressed into the hydraulic oil cylinder, and according to the fluid mechanics principle, because the two (or four) hydraulic oil cylinders are communicated, the pressure in the hydraulic oil cylinders is completely the same, so that the thrust generated by the two (or four) hydraulic oil cylinders is also the same.
Description
Technical Field
The invention relates to the technical field of chromatography, in particular to a multi-oil-cylinder pressurizing device of an OPLC (optical phase separation liquid chromatography) pressurizing thin-layer chromatograph.
Background
Thin-layer chromatography, a rapid and simple chromatography technique, has been widely used in many fields such as herbal analysis, biological extracts, and toxicant analysis. In the conventional thin layer chromatography, the separation of the components is completed by the thin layer adsorbent by means of the developing solvent through capillary action, so that the separation time cannot be long, and the diffusion of the separated components is more and more severe as the movement of the solvent front is gradually slowed down with the increase of the developing distance. In response to this situation, analysts have improved the thin layer chromatography system and developed a critical branch of thin layer chromatography, forced flow thin layer chromatography. FFPC forces the developing agent to move in the adsorbent by external force, and mainly comprises centrifugal thin layer chromatography and pressurized thin layer chromatography.
The OPLC ultra-pressurized thin layer chromatograph designs an ultra-micro expansion chamber, and the expansion chamber covers the surface of a thin layer plate by a piece of glass or plastic film during the expansion process, thereby reducing the influence of gas phase in the thin layer chromatographic separation. OPLC technology was developed based on a micro-expansion chamber by applying a certain pressure on a plastic film covering the thin-layer plate to press the expanding agent into the thin-layer plate. The OPLC directly pumps the developing agent into the thin-layer plate by a pressurizing pump, and adjusts the flow rate of the developing agent by the pressurization of the pump, so that the tested sample is directionally developed.
The existing system design adopts an ultra-thin rectangular cylinder single-piston scheme, and the stress is concentrated on the central point of the working surface of the cylinder body during pressurization, so that the central deformation of the working surface is large, and the deformation of the center of a top plate of a pressure-bearing support is larger than 2mm through test measurement; therefore, the pressure distribution of the developing agent in the flat-plate column is not balanced, the tested sample cannot be developed linearly, or a plurality of samples cannot be separated in parallel, and therefore, the multi-cylinder pressurizing device for the OPLC pressurized thin-layer chromatograph is provided.
Disclosure of Invention
The invention aims to provide a multi-cylinder pressurizing device for an OPLC (optical phase liquid chromatography) pressurized thin-layer chromatograph, which aims to solve the problems in the background art.
In order to achieve the purpose, the invention provides the following technical scheme: a multi-cylinder pressurizing device of an OPLC (optical phase separation and liquid chromatography) pressurizing thin-layer chromatograph comprises a working panel, a flat column clamping plate and a flat column, wherein the flat column clamping plate is placed at the top of the working panel, supporting rods are fixedly connected to the top of the working panel and positioned on two sides of the flat column clamping plate, a pressure-bearing support is fixedly connected to the top of each supporting rod, each flat column clamping plate consists of a thin film layer and a steel plate, the flat column is placed between each thin film layer and the corresponding steel plate, two groups of through holes are arranged on the surface of each thin film layer in a penetrating mode, the bottom of each through hole is in contact with the surface of the corresponding flat column, an expansion groove is formed in the bottom of each thin film layer and in contact with the corresponding flat column, each expansion groove is vertically arranged and in contact with the corresponding through hole, a hydraulic oil cylinder is, the bottom of the piston is in contact with the top of the through hole, the piston is rectangular, return springs are fixedly connected to four corners of the piston, the other end of each return spring is fixedly connected to the bottom of the pressure bearing support, and working spaces with the height of 20-25 mm are arranged on the top surfaces of the hydraulic oil cylinders and the pressure bearing support and used for placing the expansion chambers.
Furthermore, the outer surface of the flat plate column is provided with a sealing strip, and the manufacturing material of the sealing strip is a high polymer material.
Further, the film layer is a polytetrafluoroethylene film layer.
Further, the specification of the flat plate column is set to be 50mm × 200mm, l00mm × 200mm and 200mm × 200mm, and the size of the clamp plate of the flat plate column is matched with that of the flat plate column.
Furthermore, the hydraulic oil cylinders are arranged into two groups or four groups, and the hydraulic oil cylinders can be communicated by using internal pipelines or external pipelines.
Furthermore, the top of the through hole inputs the developing agent into the flat column through a high-pressure infusion pump, and the flowing speed of the developing agent in the through hole in the flat column is controlled by controlling the pressure of the infusion pump.
Compared with the prior art, the invention has the following beneficial effects:
1. when the expansion chamber, the flat column plate clamp and the flat column are pressurized, the stress center is two points (or four points) distributed at the center of the oil cylinder, and the deformation is obviously reduced compared with the deformation when one oil cylinder is designed originally, so that the uniformity of the pressure distribution of the expanding agent is facilitated, the tested sample is ensured to be linearly expanded along the specified direction, or a plurality of tested samples are linearly expanded in parallel, and a better component separation effect is obtained;
2. when the instrument works, the oil pump connected with the hydraulic oil cylinder is started, the hydraulic oil is pressed into the hydraulic oil cylinder, and according to the fluid mechanics principle, because the two (or four) hydraulic oil cylinders are communicated, the pressure in the hydraulic oil cylinders is completely the same, so that the thrust generated by the two (or four) hydraulic oil cylinders is also the same.
Drawings
FIG. 1 is a schematic structural front view of the present invention;
FIG. 2 is a schematic bottom view of a film layer of the present invention;
FIG. 3 is a schematic top view of a plate column according to the present invention;
FIG. 4 is a front sectional structural view of the pressure bearing bracket of the present invention.
In the figure: the device comprises a working panel 1, supporting rods 2, a pressure-bearing support 3, a flat plate column clamping plate 4, a thin film layer 5, a steel plate 6, a flat plate column 7, a hydraulic oil cylinder 8, a piston 9, a reset tension spring 10, a through hole 11, a capacity expansion groove 12 and a sealing strip 13.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Referring to fig. 1-4, the present invention provides a technical solution: a multi-cylinder pressurizing device of an OPLC (optical phase liquid chromatography) pressurizing thin-layer chromatograph comprises a working panel 1, a flat column clamping plate 4 and a flat column 7, wherein the flat column clamping plate 4 is placed at the top of the working panel 1, supporting rods 2 are fixedly connected to the top of the working panel 1 and two sides of the flat column clamping plate 4, a pressure-bearing support 3 is fixedly connected to the top of each supporting rod 2, the flat column clamping plate 4 consists of a film layer 5 and a steel plate 6, the flat column 7 is placed between the film layer 5 and the steel plate 6, two groups of through holes 11 are arranged on the surface of the film layer 5 in a penetrating mode, the bottom of each through hole 11 is in contact with the surface of the flat column 7, an expansion groove 12 is formed in the bottom of the film layer 5 and in contact with the flat column 7, the expansion groove 12 is vertically arranged and in contact with the through hole 11, a hydraulic oil cylinder 8, the bottom of the hydraulic oil cylinder 8 and the bottom of the pressure bearing support 3 are provided with pistons 9, the bottoms of the pistons 9 are in contact with the tops of the through holes 11, the pistons 9 are rectangular, return springs 10 are fixedly connected to four corners of the pistons 9, the other ends of the return springs 10 are fixedly connected to the bottom of the pressure bearing support 3, and working spaces with the height of 20-25 mm are arranged on the top surfaces of the hydraulic oil cylinder 8 and the pressure bearing support 3 and used for placing expansion chambers.
Further, the outer surface of the flat plate column 7 is provided with a sealing strip 13, and the manufacturing material of the sealing strip 13 is a high polymer material.
Further, the film layer 5 is a polytetrafluoroethylene film layer.
Further, the specification of the flat plate column 7 is set to be 50mm × 200mm, l00mm × 200mm and 200mm × 200mm, and the size of the flat plate column clamping plate 4 is matched with that of the flat plate column 7.
Further, the hydraulic oil cylinders 8 are arranged in two or four groups, and the hydraulic oil cylinders 8 can be communicated by using internal pipelines or external pipelines.
Further, the developer is supplied to the inside of the plate column 7 through the top of the through-hole 11 by a high-pressure liquid supply pump, and the flow rate of the developer in the through-hole 11 inside the plate column 7 is controlled by controlling the pressure of the liquid supply pump.
When the device is used, firstly, the flat plate column plate clamp 4 mainly comprises two layers, the upper layer is a polytetrafluoroethylene film layer 5, the lower layer is a steel plate 6, an adsorbent of the flat plate column 7 is upwards arranged between the polytetrafluoroethylene film layer 5 and the steel plate 6, the two sides of the polytetrafluoroethylene film layer 5 are respectively provided with a through hole 11, one side of the two sides of the through hole 11, which is contacted with the flat plate column 7, is carved with a diffusion groove 12, then an expanding agent pumped in by an infusion pump enters the flat plate column 7 through the two through holes 11 and the expansion grooves 12, so that the expanding agent can quickly reach the edge of the flat plate column 7, thereby ensuring that the middle and the edge of the flat plate column 7 almost start to be expanded simultaneously, the edge of the flat plate column 7 is scraped off by a circle of about 2mm wide adsorbent, then a layer of sealing strips 13 is added on the four peripheries of the high polymer material, so as to prevent the expanding agent from overflowing from the edge of the flat plate column 7 during pressure expansion, the steel plate 6 at the lower layer of the flat plate column plate clamp 4 mainly plays a supporting role, and adopts a 220mm × 100mm cuboid ultra-thin hydraulic oil cylinder 8 (a position is respectively provided with a mounting block inserted into and a pressure-relief plate clamp at the position of a position which is respectively provided with a hydraulic cylinder, so that the pressure-bearing block is equal to be connected with two hydraulic cylinders, so that the pressure-reducing pressure of the pressure-reducing pipeline is equal to the pressure-reducing pipeline, so that the pressure-reducing pipeline is effectively connected with four hydraulic cylinders 8, the pressure-reducing pipeline, four hydraulic cylinders.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (6)
1. The utility model provides a many hydro-cylinders pressure device of OPLC pressurization thin-layer chromatograph, includes working panel (1), flat column splint (4) and flat column (7), its characterized in that: the plate column clamping plate is characterized in that a plate column clamping plate (4) is placed at the top of the working panel (1), supporting rods (2) are fixedly connected to the top of the working panel (1) and located on two sides of the plate column clamping plate (4), pressure-bearing supports (3) are fixedly connected to the tops of the supporting rods (2), each plate column clamping plate (4) is composed of a thin film layer (5) and a steel plate (6), a plate column (7) is placed between each thin film layer (5) and each steel plate (6), two groups of through holes (11) are arranged on the surface of each thin film layer (5) in a penetrating mode, the bottoms of the through holes (11) are in contact with the surface of each plate column (7), an expansion groove (12) is formed in the bottom of each thin film layer (5) and in contact with the plate column (7), each expansion groove (12) is vertically arranged and is in contact with each through hole (11), a hydraulic oil cylinder (8) is, the bottom of hydraulic cylinder (8) and the bottom that is located pressure-bearing support (3) are provided with piston (9), the bottom of piston (9) contacts with the top of through hole (11), piston (9) set up to the rectangle, and the equal fixedly connected with reset spring (10) in four edges of piston (9), the other end fixed connection of reset spring (10) is in the bottom of pressure-bearing support (3), hydraulic cylinder (8) and the top surface in pressure-bearing support (3) are provided with the working space that highly is 20 to 25mm for place the expansion room.
2. The multi-cylinder pressurization device for the OPLC pressurized thin layer chromatograph of claim 1, wherein: the outer surface of the flat plate column (7) is provided with a sealing strip (13), and the manufacturing material of the sealing strip (13) is a high polymer material.
3. The multi-cylinder pressurization device for the OPLC pressurized thin layer chromatograph of claim 1, wherein: the thin film layer (5) is a polytetrafluoroethylene thin film layer.
4. The OPLC pressurized thin layer chromatograph multi-oil cylinder pressurizing device as claimed in claim 1, wherein the specifications of the flat plate column (7) are set to be 50mm × 200mm, l00mm × 200mm and 200mm × 200mm, and the size of the flat plate column clamping plate (4) is matched with that of the flat plate column (7).
5. The multi-cylinder pressurization device for the OPLC pressurized thin layer chromatograph of claim 1, wherein: the hydraulic oil cylinders (8) are arranged into two groups or four groups, and the hydraulic oil cylinders (8) can be communicated by using internal pipelines or external pipelines.
6. The multi-cylinder pressurization device for the OPLC pressurized thin layer chromatograph of claim 1, wherein: the top of the through hole (11) inputs the developing agent into the flat column (7) through a high-pressure infusion pump, and the flowing speed of the developing agent in the through hole (11) in the flat column (7) is controlled by controlling the pressure of the infusion pump.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010285908.7A CN111366674A (en) | 2020-04-13 | 2020-04-13 | Multi-oil-cylinder pressurizing device for OPLC (optical phase separation liquid chromatography) pressurized thin-layer chromatograph |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010285908.7A CN111366674A (en) | 2020-04-13 | 2020-04-13 | Multi-oil-cylinder pressurizing device for OPLC (optical phase separation liquid chromatography) pressurized thin-layer chromatograph |
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| Publication Number | Publication Date |
|---|---|
| CN111366674A true CN111366674A (en) | 2020-07-03 |
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| CN202010285908.7A Pending CN111366674A (en) | 2020-04-13 | 2020-04-13 | Multi-oil-cylinder pressurizing device for OPLC (optical phase separation liquid chromatography) pressurized thin-layer chromatograph |
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Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4469601A (en) * | 1981-03-17 | 1984-09-04 | Varex Corporation | System and apparatus for multi-dimensional real-time chromatography |
| US4591524A (en) * | 1979-06-12 | 1986-05-27 | Erno Tyihak | Chromatographic sheet of layer for pressurized layer chromatographic apparatus |
| US4671870A (en) * | 1985-04-04 | 1987-06-09 | Tompa Ildiko F | Apparatus for overpressured thin-layer chromatographic technique |
| CN1678905A (en) * | 2002-08-02 | 2005-10-05 | 比奥尼西斯股份有限公司 | Device for separating sample components by liquid chromatography under pressure |
| CN202097791U (en) * | 2011-06-24 | 2012-01-04 | 江苏森茂竹木业有限公司 | Cold press |
| CN102680639A (en) * | 2011-03-15 | 2012-09-19 | 上海高佳仪器科技有限公司 | Pressurized thin-layer chromatography development chamber |
| CN204604484U (en) * | 2015-03-04 | 2015-09-02 | 林松彬 | A kind of Novel cold press |
| CN110470784A (en) * | 2019-06-13 | 2019-11-19 | 广州科曼生物科技有限公司 | A kind of thin-layer chromatography self-presenting devices and Automatic-expanding method |
-
2020
- 2020-04-13 CN CN202010285908.7A patent/CN111366674A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4591524A (en) * | 1979-06-12 | 1986-05-27 | Erno Tyihak | Chromatographic sheet of layer for pressurized layer chromatographic apparatus |
| US4469601A (en) * | 1981-03-17 | 1984-09-04 | Varex Corporation | System and apparatus for multi-dimensional real-time chromatography |
| US4671870A (en) * | 1985-04-04 | 1987-06-09 | Tompa Ildiko F | Apparatus for overpressured thin-layer chromatographic technique |
| CN1678905A (en) * | 2002-08-02 | 2005-10-05 | 比奥尼西斯股份有限公司 | Device for separating sample components by liquid chromatography under pressure |
| CN102680639A (en) * | 2011-03-15 | 2012-09-19 | 上海高佳仪器科技有限公司 | Pressurized thin-layer chromatography development chamber |
| CN202097791U (en) * | 2011-06-24 | 2012-01-04 | 江苏森茂竹木业有限公司 | Cold press |
| CN204604484U (en) * | 2015-03-04 | 2015-09-02 | 林松彬 | A kind of Novel cold press |
| CN110470784A (en) * | 2019-06-13 | 2019-11-19 | 广州科曼生物科技有限公司 | A kind of thin-layer chromatography self-presenting devices and Automatic-expanding method |
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Application publication date: 20200703 |